CN106282067B - Multifunctional agricultural complex micro organism fungicide and probiotics and application - Google Patents
Multifunctional agricultural complex micro organism fungicide and probiotics and application Download PDFInfo
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Abstract
The invention discloses multifunctional agricultural complex micro organism fungicide and probiotics and applications.Agricultural complex micro organism fungicide provided by the present invention, its active constituent are made of A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria.Agricultural probiotics provided by the present invention, its active constituent are the cultures obtained with the agricultural complex micro organism fungicide of fermentation medium culture preceding claim.Above-mentioned agricultural complex micro organism fungicide and above-mentioned agricultural probiotics can be used for preventing and/or treating plant anthracnose, can be used for promoting crop yield.
Description
Technical field
The present invention relates to multifunctional agricultural complex micro organism fungicide and probiotics in agricultural and microorganism field with answer
With.
Background technique
China is vast in territory, and weather conditions are complicated, and the fruit tree of plantation is many kinds of, the biggish fruit tree of cultivated area have peach,
Apple etc..Peach and apple disease type are also more.Peach anthracnose is the important fruit disease of peach.The master of peach anthracnose
Endangering is fruit, can also infect the young tip and blade.Young fruit, which catches an illness to develop, to be stopped, and fruit face crineous, it is residual that atrophy hardens into mummy
It stays on branch.After Fruit, attacked fruit face is just in filbert water stain shape scab, rear to expand the brown that reddens, and scab recess has bright
Aobvious concentric wheel stripe shape wrinkle, generates Chinese red clayey granule point when humidity is big, the last soft corruption of the disease fruit mummy that falls off or is formed remains
In on branch.Young sprout is caught an illness, and in oblong brown recess scab, sick tip lateral bending is withered when serious.Blade catch an illness generate it is light
Brown circle or irregular shape taupe scab generate Chinese red to black grain point thereon.Withered fall off of scab wears gift afterwards, young sprout
Top vane atrophy is sagging, vertical to be rolled into tubulose.
Requirement with the public to " green food ", " pollution-free food " is higher and higher, market to it is pollution-free, can drop naturally
The ecological agricultural chemical demand of solution also gradually increases.From the point of view of the development of pesticide science, upper " back to nature " is used in pesticide manufacturing
And " Harmless " of pesticide, it is the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has
Wide development and application prospect.
Summary of the invention
The technical problem to be solved by the present invention is to how prevent and/or treat plant anthracnose and/or crop is promoted to increase
It produces.
In order to solve the above technical problems, the present invention provides agricultural complex micro organism fungicides.
Agricultural complex micro organism fungicide provided by the present invention, its active constituent is by A Yebo plurispore bacillus, lichens
Bacillus, Lyceum bacillus and east acetic acid bacteria composition.
In above-mentioned agricultural complex micro organism fungicide, the A Yebo plurispore bacillus is A Yebo plurispore bacillus ACCC
19947, the bacillus licheniformis is bacillus licheniformis ACCC 19946, and the Lyceum bacillus is Lyceum bacillus
ACCC 19948, the east acetic acid bacteria are east acetic acid bacteria ACCC 19949.
In above-mentioned agricultural complex micro organism fungicide, A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and
The Colony Forming Unit ratio of east acetic acid bacteria can be (2.5-4): 1:(1-2): (3-4.5).
In above-mentioned agricultural complex micro organism fungicide, A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and
The Colony Forming Unit ratio of east acetic acid bacteria can be 3.25:1:1.5:3.75.
Above-mentioned agricultural complex micro organism fungicide can be used for preventing and/or treating plant anthracnose, can be used for that crop is promoted to increase
It produces.
The A Yebo plurispore bar in above-mentioned agricultural complex micro organism fungicide or the agricultural complex micro organism fungicide
Following any applications of bacterium, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria also belong to protection model of the invention
It encloses:
1) application in preparation plant anthracnose cause of disease bacteria inhibitor；
2) preventing and/or treating the application in plant anthracnose；
3) promoting the application in crop yield；
4) application in following agricultural probiotics is being prepared.
In order to solve the above technical problems, the present invention also provides agricultural probiotics.
Agricultural probiotics provided by the present invention, its active constituent are agricultural multiple described in fermentation medium culture
Close the culture (product in culture vessel) that microbial bacterial agent obtains.
In above-mentioned agricultural probiotics, A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east
The Colony Forming Unit ratio of acetic acid bacteria is (10-30): 1:(1-5): (30-50) or 18:1:1.6:40.
In above-mentioned agricultural probiotics, the fermentation medium can be the molasses aqueous solution of various concentration, such as 6% (matter
Measure percentage composition) molasses aqueous solution.
Molasses are the byproducts of sugar industry.The general brix of molasses is 80-90Bx, contains 50% or more sugar.
In above-mentioned agricultural probiotics, what the complex micro organism fungicide agricultural described in fermentation medium culture used
Cultivation temperature can be 20-30 DEG C.
Above-mentioned agricultural probiotics can be used for preventing and/or treating plant anthracnose, can be used for promoting crop yield.
Following any applications of above-mentioned agricultural probiotics also belong to protection scope of the present invention:
1) application in preparation plant anthracnose cause of disease bacteria inhibitor；
2) preventing and/or treating the application in plant anthracnose；
3) promoting the application in crop yield.
Above, the plant can be rosaceous plant, and the crop can be Theaceae crop, solanaceous crops, legume
Or crop in cruciferae.
The rosaceous plant can be peach, and the Theaceae crop can be tea, and the solanaceous crops can be capsicum, the pulse family
Crop can be cowpea, and the crop in cruciferae can be wild cabbage.
Above-mentioned agricultural complex micro organism fungicide and above-mentioned agricultural probiotics may also include carrier, and preferably pesticide field is normal
It and is being biologically inert carrier.The carrier can be solid carrier or liquid-carrier.The solid carrier is mine
Object material, vegetable material or high-molecular compound；The mineral material is clay, talcum, kaolin, montmorillonite, white carbon, boiling
At least one of stone, silica and diatomite；The vegetable material is at least one of corn flour, bean powder and starch；It is described
High-molecular compound is polyvinyl alcohol and/or polyglycols.The liquid-carrier is organic solvent, vegetable oil, mineral oil or water；Institute
Stating organic solvent is decane and/or dodecane.In the microbial inoculum, the active constituent can living cells, living thin to be cultured
The form of the fermentation liquid of born of the same parents, the filtrate of cell culture or cell and the mixture of filtrate exists.The living cells can be mitogenetic
Spore, chlamydospore, mycelia or the mycelial form containing conidium and mycelia, preferably conidium or chlamydospore
Form.The dosage form of the composition can be a variety of dosage forms, such as liquor, emulsion, suspending agent, pulvis, granule, wettable powder
Or water dispersible granules.
As needed, surface-active can also be added in above-mentioned agricultural complex micro organism fungicide and above-mentioned agricultural probiotics
Agent (such as polysorbas20, Tween 80), adhesive, stabilizer (such as antioxidant), pH adjusting agent.
It is demonstrated experimentally that the agricultural probiotics pair that the agricultural complex micro organism fungicide described in fermentation medium culture obtains
The preventive effect of peach anthracnose reaches 92.39%, reaches 83.65% to the therapeutic effect of peach anthracnose, the increasing to tealeaves
Yield reaches 12%, distinguishes 20% and 15% to the rate of growth of capsicum, cowpea, cabbage head diameter is made to increase 4-6cm.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
A Yebo plurispore bacillus ACCC 19947 in following embodiments is A Yebo plurispore bacillus (Bacillus
Aryabhattai) R002 was concealed in China Committee for Culture Collection of Microorganisms agricultural microorganism on August 1st, 2016
Center (abbreviation ACCC, address: No.12 ,zhongguancun south street,Haidian District, Beijing, Chinese Academy of Agricultural Sciences's agricultural resource and agricultural
Research on zoning institute, postcode 100081), the public can be micro- from China Committee for Culture Collection of Microorganisms's agricultural from the collection day
Bio-Centers obtain the bacterial strain.
Bacillus licheniformis ACCC 19946 in following embodiments is bacillus licheniformis (Bacillus
Licheniformis) R001 has been concealed in the micro- life of China Committee for Culture Collection of Microorganisms's agricultural on August 1st, 2016
Object center (abbreviation ACCC, address: No.12 ,zhongguancun south street,Haidian District, Beijing, Chinese Academy of Agricultural Sciences's agricultural resource and agriculture
Industry Research on zoning institute, postcode 100081), the public can be from China Committee for Culture Collection of Microorganisms's agricultural from the collection day
Microorganism center obtains the bacterial strain.
Lyceum bacillus ACCC 19948 in following embodiments is Lyceum bacillus (Bacillus
Siamensis) R003 was concealed in China Committee for Culture Collection of Microorganisms agricultural microorganism on August 1st, 2016
The heart (abbreviation ACCC, address: No.12 ,zhongguancun south street,Haidian District, Beijing, Chinese Academy of Agricultural Sciences's agricultural resource and farming region
Draw research institute, postcode 100081), the public can be from the micro- life of China Committee for Culture Collection of Microorganisms's agricultural from the collection day
Object center obtains the bacterial strain.
East acetic acid bacteria ACCC 19949 in following embodiments is east acetic acid bacteria (Acetobacter
Orientalis) R004 was concealed in China Committee for Culture Collection of Microorganisms agricultural microorganism on August 1st, 2016
Center (abbreviation ACCC, address: No.12 ,zhongguancun south street,Haidian District, Beijing, Chinese Academy of Agricultural Sciences's agricultural resource and agricultural
Research on zoning institute, postcode 100081), the public can be micro- from China Committee for Culture Collection of Microorganisms's agricultural from the collection day
Bio-Centers obtain the bacterial strain.
A Yebo plurispore bacillus ACCC 19947, bacillus licheniformis ACCC 19946 and Lyceum bacillus ACCC
19948 use the culture of nutrient culture medium.Nutrient culture medium is as follows:
Nutrient solid medium: peptone 5g, NaCl 5g, meat extract 3g, agar 15g are settled to distilled water
1000ml, pH7.0.It sterilizes 20 minutes under the conditions of 121 DEG C.
Nutrient fluid nutrient medium: peptone 5g, NaCl 5g, meat extract 3g are settled to 1000ml with distilled water,
pH7.0.It sterilizes 20 minutes under the conditions of 121 DEG C.
East acetic acid bacteria ACCC 19949 uses the culture of acetic acid bacterium culture medium, and acetic acid bacterium culture medium is as follows:
Acetic acid bacteria solid medium: glucose 100g, yeast extract 10g, CaCO320g, agar 15g, with distilled water constant volume
To 1000ml, pH6.8.It sterilizes 20 minutes under the conditions of 121 DEG C.
Acetic acid bacteria fluid nutrient medium are as follows: glucose 100g, yeast extract 10g, CaCO320g is settled to distilled water
1000ml, pH6.8.It sterilizes 20 minutes under the conditions of 121 DEG C.
The preparation of embodiment 1, multifunctional agricultural complex micro organism fungicide
1, suspend mode actication of culture
By A Yebo plurispore bacillus ACCC 19947, the bacillus licheniformis ACCC 19946, Lyceum bud of dormant state
Spore bacillus ACCC 19948 and east acetic acid bacteria ACCC 19949 is respectively connected to test tube slant activation；A Yebo plurispore bacillus
ACCC 19947, bacillus licheniformis ACCC 19946 and Lyceum bacillus ACCC 19948 use nutrient solid culture
Base culture, 28-30 DEG C of cultivation temperature, culture 24-48 hours；East acetic acid bacteria ACCC 19949 uses acetic acid bacteria solid medium
Culture, 28-30 DEG C of cultivation temperature, continuous culture 24-48 hours.
2, the preparation of multifunctional agricultural complex micro organism fungicide
This step provides two kinds of multifunctional agricultural complex micro organism fungicides, respectively multifunctional agricultural composite microbial bacteria
Agent A and multifunctional agricultural complex micro organism fungicide B.The active constituent of multifunctional agricultural complex micro organism fungicide A is by A Yeboduo
Bacillus ACCC 19947, bacillus licheniformis ACCC 19946, Lyceum bacillus ACCC 19948 and east acetic acid bacteria
ACCC 19949 is formed.In multifunctional agricultural complex micro organism fungicide A, A Yebo plurispore bacillus ACCC 19947, lichens
The Colony Forming Unit of bacillus ACCC 19946, Lyceum bacillus ACCC 19948 and east acetic acid bacteria ACCC 19949
(cfu) than being 13:4:6:15.The active constituent of multifunctional agricultural complex micro organism fungicide B is by A Yebo plurispore bacillus ACCC
19947, bacillus licheniformis ACCC 19946 and Lyceum bacillus ACCC 19948 is formed.The multifunctional agricultural composite microbial
In object microbial inoculum B, A Yebo plurispore bacillus ACCC 19947, bacillus licheniformis ACCC 19946 and Lyceum bacillus ACCC
19948 Colony Forming Unit (cfu) is than being 13:4:6.
It is specific the preparation method is as follows:
By the access of A Yebo plurispore bacillus ACCC 19947 after step 1 activation equipped with the nutrient liquid after sterilizing
The shaking flask (500ml triangular flask is equipped with 100mL culture medium, and revolving speed is 180rpm) of culture medium, bacillus licheniformis ACCC 19946
(500ml triangular flask is equipped with 100mL culture medium to shaking flask of the access equipped with the nutrient fluid nutrient medium after sterilizing, and revolving speed is
180rpm), shaking flask (500ml of the access of Lyceum bacillus ACCC 19948 equipped with the nutrient fluid nutrient medium after sterilizing
Triangular flask be equipped with 100mL culture medium, revolving speed is 180rpm), east acetic acid bacteria ACCC 19949 access equipped with sterilizing after acetic acid
The shaking flask (500ml triangular flask is equipped with 100mL culture medium, and revolving speed is 180rpm) of bacteria liquid culture medium, is then placed in constant temperature oscillation
Device is cultivated, and 28-30 DEG C of temperature, culture 15-48 hours respectively obtain the liquid of A Yebo plurispore bacillus ACCC 19947
Strain, the liquid spawn of bacillus licheniformis ACCC 19946, the liquid spawn of Lyceum bacillus ACCC 19948, east vinegar
The liquid spawn of sour bacterium ACCC 19949.A Yebo polygerm in the liquid spawn of the A Yebo plurispore bacillus ACCC 19947
19947 content of spore bacillus ACCC is 13 × 108Cfu/ml, the ground in the liquid spawn of the bacillus licheniformis ACCC 19946
19946 content of clothing bacillus ACCC is 4 × 108Cfu/ml, the Lyceum bacillus ACCC 19948 liquid spawn in
19948 content of Lyceum bacillus ACCC is 6 × 108Cfu/ml, east acetic acid bacteria ACCC 19949 liquid spawn in east
Square 19949 content of acetic acid bacteria ACCC is 15 × 108cfu/ml。
By the liquid spawn of above-mentioned A Yebo plurispore bacillus ACCC 19947, the liquid of bacillus licheniformis ACCC 19946
The liquid spawn of body strain, the liquid spawn of Lyceum bacillus ACCC 19948 and east acetic acid bacteria ACCC 19949 according to etc.
Volume mixture obtains multifunctional agricultural complex micro organism fungicide A.In multifunctional agricultural complex micro organism fungicide A, A Yeboduo
Bacillus ACCC 19947, bacillus licheniformis ACCC 19946, Lyceum bacillus ACCC 19948 and east acetic acid bacteria
The Colony Forming Unit (cfu) of ACCC 19949 is than being 13:4:6:15.
By the liquid spawn of above-mentioned A Yebo plurispore bacillus ACCC 19947, the liquid of bacillus licheniformis ACCC 19946
The liquid spawn of body strain and Lyceum bacillus ACCC 19948 are mixed to get multifunctional agricultural composite microbial according to isometric
Object microbial inoculum B.In multifunctional agricultural complex micro organism fungicide B, A Yebo plurispore bacillus ACCC 19947, bacillus licheniformis
The Colony Forming Unit (cfu) of ACCC 19946 and Lyceum bacillus ACCC 19948 is than being 13:4:6.
The preparation of embodiment 2, multifunctional agricultural probiotics
Present embodiments provide two kinds of multifunctional agricultural probiotics, be respectively multifunctional agricultural probiotics A and
Multifunctional agricultural probiotics B.
1, the preparation of multifunctional agricultural probiotics A
The molasses aqueous solution that 50 liters of concentration are 6% (mass percentage) is added in 100 liters of seeding tanks, it, will after sterilizing
The multifunctional agricultural complex micro organism fungicide A of embodiment 1 is accessed in 100 liters of seeding tanks, inoculum concentration 0.8%-1%, ventilation
Amount is 0.5 (V/Vmin) (being passed through the volume of air of fermentor and the ratio of fermentation cylinder for fermentation liquid product per minute), revolving speed
To cultivate 24 hours under the conditions of 200rpm, 30 DEG C, seed liquor is obtained.
The molasses aqueous solution that 500 liters of concentration are 6% (mass percentage) is added in 1000 liters of fermentors, after sterilizing,
Seed liquor is accessed in 1000 liters of fermentors and be fermented, inoculum concentration is that 10% (seed liquor is culture volume in fermentor
10%), ventilation ratio ventilatory capacity is that 0.5 (V/Vmin) (is passed through the volume of air and fermentation cylinder for fermentation liquid of fermentor per minute
The ratio of volume), revolving speed 200rpm, control pH7.0-7.2,30 DEG C cultivate 24 hours, be added volume be liquid in fermentor
4% molasses of volume carry out first time feed supplement, and uncomfortable pH after first time feed supplement is complete, temperature, revolving speed, ventilatory capacity are constant after supervention
The secondary feed supplement of 5% molasses progress that volume is liquid volume in fermentor, secondary benefit is added after reaching within ferment 18 hours stationary phase
Expect to adjust pH to 4.5-5.0, other parameters are constant to be continued to ferment 18 hours and reach stationary phase, by the bacterium solution of the stationary phase and sterile
Water is mixed to get multifunctional agricultural probiotics A, and wherein bacterium solution and the volume ratio of sterile water are 1:4.The multifunctional agricultural is micro-
In ecological agent A, 19947 content of A Yebo plurispore bacillus ACCC is 9 × 108Cfu/mL, Lyceum bacillus ACCC
19948 contents are 8.0 × 10719946 content of cfu/mL, bacillus licheniformis ACCC is 5.0 × 107Cfu/mL, east acetic acid
19949 content of bacterium ACCC is 2 × 109cfu/mL.In multifunctional agricultural probiotics A, A Yebo plurispore bacillus ACCC
19947, the bacterium of bacillus licheniformis ACCC 19946, Lyceum bacillus ACCC 19948 and east acetic acid bacteria ACCC 19949
It falls to form unit (cfu) than being 18:1:1.6:40.
2, the preparation of multifunctional agricultural probiotics B
The multifunctional agricultural complex micro organism fungicide A of embodiment 1 in step 1 is replaced with to the multi-functional agriculture of embodiment 1
It is other constant with complex micro organism fungicide B, obtain multifunctional agricultural probiotics B.In multifunctional agricultural probiotics B,
19947 content of A Yebo plurispore bacillus ACCC is 9 × 10819948 content of cfu/mL, Lyceum bacillus ACCC is 8.0
×10719946 content of cfu/mL, bacillus licheniformis ACCC is 5.0 × 107cfu/mL.Multifunctional agricultural probiotics B
In, A Yebo plurispore bacillus ACCC 19947, bacillus licheniformis ACCC 19946 and Lyceum bacillus ACCC 19948
Colony Forming Unit ratio be 18:1:1.6.
Embodiment 3, multifunctional agricultural probiotics are to the prevention and treatment of peach anthracnose
Using the multifunctional agricultural of 200 times of dilutions and embodiment 2 of the multifunctional agricultural probiotics A of embodiment 2
200 times of dilutions of probiotics B carry out the prevention and treatment of peach anthracnose.The multifunctional agricultural Tiny ecosystem of embodiment 2
200 times of dilutions of preparation A are that the multifunctional agricultural probiotics A of embodiment 2 is diluted 200 times of obtained liquid with sterile water
Body.200 times of dilutions of the multifunctional agricultural probiotics B of embodiment 2 are with sterile water by the multifunctional agricultural of embodiment 2
Probiotics B dilutes 200 times of obtained liquid.
1, the prevention of peach anthracnose
Every year all can by a peach garden of the Shandong Province that anthracnose is invaded in various degree, select the age of tree for 6 years 300
The similar peach of growth conditions (kind is honey peach), is randomly divided into three groups, experimental group A, experimental group B and control group, and every group
100.The multifunctional agricultural that every peach of experimental group A sprays an embodiment 2 to tree body (at the beginning of 3 months) before spring rudiment is micro-
200 times of dilutions of ecological agent A spray the 200 of the multifunctional agricultural probiotics A an of embodiment 2 to tree body before spending
Times dilution, sprays 200 times of dilutions of the multifunctional agricultural probiotics A an of embodiment 2, in first fruit to tree body after spending
Phase sprays 200 times of dilutions of the multifunctional agricultural probiotics A an of embodiment 2, sprays primary reality in fruit expanding period
Apply 200 times of dilutions of the multifunctional agricultural probiotics A of example 2.Experimental group B remove by experimental group A embodiment 2 it is multi-functional
200 times of dilutions of agricultural probiotics A replace with 200 times of the multifunctional agricultural probiotics B of the embodiment 2 of equivalent
Outside dilution, other operations are all the same.Control group is removed the multifunctional agricultural probiotics A of embodiment 2 in experimental group A
200 times of dilutions replace with outside the sterile water of equivalent, and other operations are all the same.The investigation morbidity feelings after spraying 7 days for the last time
Condition records diseased plant number and state of an illness rank, calculates disease index and preventive effect.Preventive effect %=(control group disease index-experimental group the state of an illness
Index)/control group disease index × 100, disease index=[∑ (diseased plant numbers at different levels × typical value at different levels)/total strain number × 5] ×
100。
Peach anthracnose grade scale is as follows:
0 grade: disease-free；
1 grade: having a small amount of scab, lesion area accounts for fruit and 1/16 or less leaf area；
2 grades: more scab, lesion area account for 1/16 to 1/8 (not including 1/16) of fruit and leaf area；
3 grades: lesion area accounts for 1/8 to 1/4 (not including 1/8) of fruit and leaf area；
4 grades: lesion area accounts for 1/4 to 1/2 (not including 1/4) of fruit and leaf area；
5 grades: lesion area accounts for the 1/2 to 3/4 of fruit and leaf area.
The preventive effect of different disposal group peach anthracnose is as shown in table 1, shows the multifunctional agricultural Tiny ecosystem of embodiment 2
Preparation A reaches 92.39% to the preventive effect of peach anthracnose, and the multifunctional agricultural probiotics B of embodiment 2 is to peach charcoal
The preventive effect of subcutaneous ulcer disease reaches 62.24%.
The preventive effect of table 1, peach anthracnose
| Processing | Disease index | Preventive effect |
| Control group | 24.31 | |
| Experimental group A | 1.85 | 92.39% |
| Experimental group B | 9.18 | 62.24% |
2, the treatment of peach anthracnose
Every year all can by a peach garden of the Shandong Province that anthracnose is invaded in various degree, select the age of tree for 4 years 90
The first fruiting period peach (kind is nectarine) that growth conditions are similar, the sick grade of anthracnose is 1 grade, is randomly divided into three groups, experimental group
A, experimental group B and control group, every group 30.The multifunctional agricultural that experimental group A sprayed an embodiment 2 to tree body every 7 days is micro-
200 times of dilutions of ecological agent A, spray 5 times altogether.Experimental group B is removed the micro- life of the multifunctional agricultural of embodiment 2 in experimental group A
200 times of dilutions of state preparation A replace with 200 times of dilutions of the multifunctional agricultural probiotics B of the embodiment 2 of equivalent
Outside, other operations are all the same.Control group is removed 200 times of dilutions of the multifunctional agricultural probiotics A of embodiment 2 in experimental group
Liquid replaces with outside the sterile water of equivalent, and other operations are all the same.Incidence is investigated after spraying 7 days for the last time, calculates disease
Feelings index and curative effect.The calculation method of disease index is the same as step 1.(state of an illness refers to curative effect %=after disease index-treatment before treating
Number)/treat preceding disease index × 100.
The grade scale of peach anthracnose is the same as step 1.
The therapeutic effect of different disposal group peach anthracnose is as shown in table 2, shows the multifunctional agricultural Tiny ecosystem of embodiment 2
Preparation A reaches 83.65% to the therapeutic effect of peach anthracnose, and the multifunctional agricultural probiotics B of embodiment 2 is to peach charcoal
Subcutaneous ulcer disease does not have therapeutic effect.
The therapeutic effect of table 2, peach anthracnose
| Processing | Disease index before treating | Disease index after treatment | Curative effect |
| Control group | 20 | 40.58 | |
| Experimental group A | 20 | 3.27 | 83.65% |
| Experimental group B | 20 | 26.75 |
It is demonstrated experimentally that the multifunctional agricultural probiotics A of embodiment 2 can not only efficiently pre- preventing peach anthracnose, also
Peach anthracnose can be effectively treated.
Embodiment 4, multifunctional agricultural probiotics promote tea tree volume increase
Using the multifunctional agricultural of 300 times of dilutions and embodiment 2 of the multifunctional agricultural probiotics A of embodiment 2
300 times of dilutions of probiotics B carry out following experiments.300 times of the multifunctional agricultural probiotics A of embodiment 2 are dilute
Releasing liquid is that the multifunctional agricultural probiotics A of embodiment 2 is diluted 300 times of obtained liquid with sterile water.Embodiment 2 it is more
300 times of dilutions of function agricultural probiotics B are with sterile water that the multifunctional agricultural probiotics B of embodiment 2 is dilute
Release 300 times of obtained liquid.
In the tea place of District, Xinyang Area, Henan Province, these three special early Xinyang Maojian Tea product of above-mentioned Xinyang 10, Fuding great Bai and Pingyang are selected
Kind tea tree, it is 90 of 5 years tea trees that each kind, which select the similar age of tree of growth conditions, is randomly divided into three groups, tests
Group A, experimental group B and control group, every group 30.Experimental group A sprays the multi-functional of an embodiment 2 to tree body when sprouting sprouts
300 times of dilutions of agricultural probiotics A spray more function an of embodiment 2 for first 10 days in each harvesting to tree body later
300 times of dilutions of the agricultural probiotics A of energy.Experimental group B is removed the multifunctional agricultural Tiny ecosystem of embodiment 2 in experimental group A
300 times of dilutions of preparation A replace with outside 300 times of dilutions of multifunctional agricultural probiotics B of the embodiment 2 of equivalent,
Other operations are all the same.Control group is removed 300 times of dilutions of the multifunctional agricultural probiotics A of embodiment 2 in experimental group
It replaces with outside the sterile water of equivalent, other operations are all the same.After tealeaves harvests, each kind experimental group and control group are counted
Tea yield, count rate of growth.Rate of growth=(experimental group yield-control group yield)/control group yield.As a result such as 3 institute of table
Show, shows that the multifunctional agricultural probiotics A of embodiment 2 has good volume increase to the tea yield of the tea tree of different cultivars
Effect, average rate of growth are 12%.The multifunctional agricultural probiotics B of embodiment 2 produces the tealeaves of the tea tree of different cultivars
Amount has effect of increasing production, and average rate of growth is 5%.The multifunctional agricultural probiotics A of embodiment 2 to improve tea leaf quality,
Improving millet paste concentration and flavour has good effect.After use, spring tea sprouts early, and bud head is more, it is good to hold Tender, and picking batch is more,
Raising spring tea Famous High-quality Tea ratio and economic benefit are increased extremely advantageous.
The tealeaves rate of growth of table 3, each processing group
Embodiment 5, multifunctional agricultural probiotics promote vegetable increase production
Following experiments are carried out using 300 times of dilutions of the multifunctional agricultural probiotics A of embodiment 2.Embodiment 2
300 times of dilutions of multifunctional agricultural probiotics A are the multifunctional agricultural probiotics A with sterile water by embodiment 2
Dilute 300 times of obtained liquid.
In Hebei the excellent extraordinary industry of favour to capsicum, cowpea and wild cabbage using embodiment 2 multifunctional agricultural probiotics A into
Row test, achieves apparent effect of increasing production.
Capsicum test uses RANDOMIZED BLOCK DESIGN, is randomly provided 2 treatment regions, each treatment region sets three repetitions.At 2
Managing area is respectively control treatment area and multifunctional agricultural probiotics A treatment region (experiment process area).The area of each cell
It is 3 mu.Multifunctional agricultural probiotics A treatment region sprays the more of an embodiment 2 after Hot Pepper Seedling transplant survival, to plant
300 times of dilutions of function agricultural probiotics A, it is micro- in the multifunctional agricultural that setting phase sprays an embodiment 2 to plant
300 times of dilutions of ecological agent A spray the multifunctional agricultural probiotics A an of embodiment 2 in the best fruiting period to plant
300 times of dilutions.Remove the multifunctional agricultural of embodiment 2 in multifunctional agricultural probiotics A treatment region is micro- in control treatment area
300 times of dilutions of ecological agent A replace with outside the sterile water of equivalent, and other operations are all the same.After capsicum harvests, statistics
The yield of hot pepper in experiment process area and control treatment area counts rate of growth.Rate of growth=(experiment process area yield-control treatment
Area's yield)/control treatment area yield.
The result shows that compared with capsicum control treatment area, the rate of growth of multifunctional agricultural probiotics A treatment region capsicum
It is 20%.
Cowpea (kind is asparagus bean) test uses RANDOMIZED BLOCK DESIGN, is randomly provided 2 treatment regions, each treatment region is set
Three repetitions.2 treatment regions are respectively control treatment area and multifunctional agricultural probiotics A treatment region (experiment process area).
The area of each cell is 3 mu.Multifunctional agricultural probiotics A treatment region sprays primary implementation to plant in the seedling stage of cowpea
300 times of dilutions of the multifunctional agricultural probiotics A of example 2 spray plant after cowpea enters a pod phase and implement twice
300 times of dilutions of the multifunctional agricultural probiotics A of example 2, this spray twice between be spaced 10 days.Control treatment area removes will
300 times of dilutions replacement of the multifunctional agricultural probiotics A of embodiment 2 in multifunctional agricultural probiotics A treatment region
Outside for the sterile water of equivalent, other operations are all the same.After cowpea harvests, the cowpea of statistical experiment treatment region and control treatment area
Beans yield counts rate of growth.Rate of growth=(experiment process area yield-control treatment area yield)/control treatment area yield.
The result shows that compared with control treatment area, multifunctional agricultural probiotics A treatment region cowpea rate of growth is
15%.Multifunctional agricultural probiotics A can delay cowpea aging, increase the yield of the fresh cowpea of secondary recovery.
Wild cabbage (in kind sweet No. 11) test uses RANDOMIZED BLOCK DESIGN, is randomly provided 2 treatment regions, each treatment region is set
Three repetitions.2 treatment regions are respectively control treatment area and multifunctional agricultural probiotics A treatment region (experiment process area).
The area of each cell is 1 mu.Multifunctional agricultural probiotics A treatment region sprays plant after cabbage seedling transplant survival
300 times of dilutions of the multifunctional agricultural probiotics A of embodiment 2 spray an embodiment 2 to plant in the rosette state
Multifunctional agricultural probiotics A 300 times of dilutions, the multi-functional agriculture an of embodiment 2 is sprayed to plant in the heading stage
With 300 times of dilutions of probiotics A.It removes embodiment 2 in multifunctional agricultural probiotics A treatment region in control treatment area
300 times of dilutions of multifunctional agricultural probiotics A replace with outside the sterile water of equivalent, other operations are all the same.As a result
Show that the cabbage head diameter of multifunctional agricultural probiotics A treatment region is 4- bigger than the cabbage head diameter for compareing treatment region
6cm.Illustrate that multifunctional agricultural probiotics A has the function of that multiple rush leaf, Ye great, leaf is thick, packet is big to leaf vegetables.
It is demonstrated experimentally that the multifunctional agricultural probiotics A of embodiment 2 synthesizes enzyme system substance in crop, activity increases
Adding has adjustment effect, has growth-promoting degeneration-resistant crop, and strong sprout is immune, spend more, it is more it is real, more, improve yield, improve quality it
Effect.
Claims (10)
1. agricultural complex micro organism fungicide, its active constituent is by A Yebo plurispore bacillus, bacillus licheniformis, Lyceum gemma
Bacillus and east acetic acid bacteria composition；The A Yebo plurispore bacillus is A Yebo plurispore bacillus ACCC 19947, describedly
Clothing bacillus is bacillus licheniformis ACCC 19946, and the Lyceum bacillus is Lyceum bacillus ACCC 19948,
The east acetic acid bacteria is east acetic acid bacteria ACCC 19949.
2. agricultural complex micro organism fungicide according to claim 1, it is characterised in that: the agricultural complex micro organism fungicide
In, the A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria Colony Forming Unit ratio
For (2.5-4): 1:(1-2): (3-4.5).
3. agricultural complex micro organism fungicide according to claim 1 or 2, it is characterised in that: the agricultural complex microorganism
In microbial inoculum, the A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria bacterium colony form list
Position is than being 3.25:1:1.5:3.75.
4. following any applications of agricultural complex micro organism fungicide described in claim 3:
1) application in preparation plant anthracnose cause of disease bacteria inhibitor；
2) preventing and/or treating the application in plant anthracnose；
3) promoting the application in crop yield；
4) application in agricultural probiotics is being prepared.
5. application according to claim 4, it is characterised in that: the plant is rosaceous plant, and the crop is Theaceae
Crop, solanaceous crops, legume or crop in cruciferae.
6. agricultural probiotics, its active constituent is with any described agricultural in fermentation medium culture claim 1-3
The culture that complex micro organism fungicide obtains.
7. agricultural probiotics according to claim 6, it is characterised in that: described in the agricultural probiotics
A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria Colony Forming Unit ratio be (10-
30): 1:(1-5): (30-50).
8. agricultural probiotics according to claim 7, it is characterised in that: described in the agricultural probiotics
A Yebo plurispore bacillus, bacillus licheniformis, Lyceum bacillus and east acetic acid bacteria Colony Forming Unit ratio be 18:1:
1.6:40.
9. following any applications of agricultural probiotics according to any one of claims 8:
1) application in preparation plant anthracnose cause of disease bacteria inhibitor；
2) preventing and/or treating the application in plant anthracnose；
3) promoting the application in crop yield.
10. application according to claim 9, it is characterised in that: the plant is rosaceous plant, and the crop is Theaceae
Crop, solanaceous crops, legume or crop in cruciferae.
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| CN108849986B (en) * | 2018-07-09 | 2020-09-15 | 江苏师范大学 | Application of bacillus simmer in preventing and treating white rot |
| CN109619283B (en) * | 2019-01-04 | 2022-10-04 | 大连民族大学 | Method for improving fermentation efficiency of soybean meal |
| CN112063552B (en) * | 2020-09-04 | 2022-08-23 | 山东初农生物科技有限公司 | Microbial agent for preventing and treating asparagus wilt, preparation method and application thereof |
| CN112209778A (en) * | 2020-09-26 | 2021-01-12 | 上海康恒环境修复有限公司 | Novel slag soil conditioner and preparation method thereof |
| CN115627247B (en) * | 2022-12-21 | 2023-04-28 | 中国热带农业科学院三亚研究院 | Preparation method and application of compound microbial fertilizer |
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