CN112063552B - Microbial agent for preventing and treating asparagus wilt, preparation method and application thereof - Google Patents
Microbial agent for preventing and treating asparagus wilt, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a microbial agent for preventing and treating asparagus wilt, a preparation method and application thereof. The application provides a compound microorganism bacterium liquid and a bacterium agent, wherein the components of the bacterium liquid consist of a bacillus subtilis liquid, a bacillus siemens liquid, an acetobacter orientalis liquid and a saccharomyces cerevisiae liquid; the microbial inoculum is prepared by fermenting and enlarging culturing the mixed bacterial liquid. In addition, the application discovers that the compound microbial agent can be used for treating asparagus wilt and promoting crop yield increase.
Description
Technical Field
The invention belongs to the field of agricultural microorganisms, and particularly relates to a microbial agent and a biochar-based compound microbial agent for preventing and treating asparagus wilt, as well as a preparation method and application thereof.
Background
According to long-term fertilizer positioning tests of national fertilizer test nets and national soil fertility and fertilizer benefit monitoring data, in recent years, the content of organic matters in soil of cultivated land in China has a tendency to decrease, the soil buffering capacity is weakened, the number and structure of soil microbial populations change, the number of harmful microbes is increased, the disaster resistance is reduced, the fertilizer utilization rate is low, the soil fertility is reduced, and crop yield reduction is caused.
The asparagus is one of the important vegetables in China, is delicious in taste, soft and tasty, is rich in dietary fibers and can promote digestion, and in addition, according to modern nutritional analysis, the asparagus protein composition has various amino acids necessary for human bodies, particularly asparagine and trace elements such as selenium, molybdenum, chromium, manganese and the like in the asparagus, and has the effects of regulating the metabolism of the organisms, improving the immunity of the bodies and the like. However, asparagus is easy to infect asparagus wilt in the planting process, and at present, the asparagus is often prevented and treated by adopting seed treatment, such as fumigation treatment by methyl bromide and ethylene oxide, and although the method has obvious sterilization effect on germs, the method also has serious phytotoxicity on seeds.
In order to solve the problems, the invention achieves the effect of reasonably utilizing agricultural waste resources by preparing the biochar-based compound microbial inoculant, really realizes the purpose of changing the agricultural waste into 'waste' and the purpose of fertilizing the waste, and the produced compound microbial fertilizer can effectively improve the content of organic carbon in soil, has good improvement effect on the aspects of reducing the loss of soil nutrients and the like while gradually improving the fertilizer and water retention performance of the soil, and has the effects of directly or indirectly improving the soil, recovering the land fertility, maintaining the balance of rhizosphere microflora, degrading toxic substances, preventing asparagus blight and the like.
Disclosure of Invention
The invention aims to provide a compound microorganism bacterium liquid, which comprises the components of bacillus subtilis liquid, bacillus siemens liquid, acetobacter orientalis liquid and saccharomyces cerevisiae liquid.
Further, in the compound microorganism liquid, bacillus subtilis is bacillus subtilis ACCC10634, bacillus cimicifuga is bacillus cimicifuga ACCC19948, acetobacter orientalis is acetobacter orientalis ACCC19949, and saccharomyces cerevisiae ACCC 21184.
Furthermore, in the composite microbial liquid, the colony forming unit ratio of bacillus subtilis, bacillus siemens, acetobacter orientalis and saccharomyces cerevisiae is 1-10:1-10:1-10:1-10, preferably, the colony forming unit ratio is 3.2:2.5:5.8: 6.1.
Further, in the compound microorganism bacterial liquid, the content of bacillus subtilis ACCC10634 is 1-10 multiplied by 10 8 cfu/ml, content of Bacillus Simmer ACCC19948 is 1-10 × 10 8 cfu/ml, and the content of Acetobacter orientalis ACCC19949 is 1-10 × 10 8 cfu/ml, Saccharomyces cerevisiae ACCC21184 content of 1-10 × 10 8 cfu/ml。
The invention aims to provide a preparation method of the compound microbial liquid, which comprises the following steps:
firstly, inoculating bacillus subtilis ACCC10634, bacillus calmette-guerin ACCC19948, acetobacter orientalis ACCC19949 and saccharomyces cerevisiae ACCC21184 in a dormant state to a test tube slant containing a solid culture medium respectively for activation;
(II) inoculating the activated bacteria into shake flasks containing liquid culture media respectively for culture;
and (III) mixing the four liquid strains obtained in the step (II) according to the equal volume to obtain the compound microbial liquid.
Further, the solid media were: bacillus subtilis and Bacillus Simmer are cultured in nutrient broth solid culture medium, Acetobacter orientalis is cultured in MRS solid culture medium, and Saccharomyces cerevisiae is cultured in malt extract solid culture medium.
Further, the liquid culture mediums in the step (two) are respectively: bacillus subtilis and Bacillus Simmer are cultured in nutrient broth liquid culture medium, Acetobacter orientalis is cultured in MRS liquid culture medium, and Saccharomyces cerevisiae is cultured in malt juice liquid culture medium.
Further, the culture temperature in the step (II) is 20-30 ℃, the culture time is 18-72 hours, and the rotation speed is 180-220 rpm.
The invention aims to provide a compound microbial agent, the active ingredients of which are prepared by mixing, fermenting and enlarging culturing bacillus subtilis, bacillus caldovelox, acetobacter orientalis and saccharomyces cerevisiae.
Further, in the compound microbial agent, bacillus subtilis is bacillus subtilis ACCC10634, bacillus cimicifuga is bacillus cimicifuga ACCC19948, acetobacter orientalis is acetobacter orientalis ACCC19949, and saccharomyces cerevisiae ACCC 21184.
Further, in the compound microbial agent, the colony forming unit ratio of bacillus subtilis, bacillus siemens, acetobacter orientalis and saccharomyces cerevisiae is 13:0.5:0.6: 0.9.
the invention aims to provide a preparation method of the compound microbial agent, which comprises the following steps:
firstly, inoculating bacillus subtilis ACCC10634, bacillus calmette-guerin ACCC19948, acetobacter orientalis ACCC19949 and saccharomyces cerevisiae ACCC21184 in a dormant state to a test tube slant containing a solid culture medium respectively for activation;
(II) inoculating the activated bacteria into shake flasks containing liquid culture media respectively for culture;
thirdly, mixing the four liquid strains obtained by the culture in the step two according to the equal volume to obtain a compound microorganism bacterial solution;
fourthly, adding molasses water solution into a seeding tank, and inoculating the compound microbial liquid prepared in the third step into the seeding tank after sterilization, wherein the inoculation amount is 0.8% -1%, so as to obtain a seed liquid;
and fifthly, adding molasses water solution into a fermentation tank, sterilizing, inoculating the seed solution prepared in the step four into the fermentation tank for fermentation, controlling the inoculation amount to be 10 percent and the pH value to be 7.0-7.2, culturing at 26-32 ℃, supplementing materials twice in the culture process, finishing the second supplementing, adjusting the pH value to be 4.5-5.0, and finishing the fermentation of the compound microbial agent after the fermentation reaches a stable period.
And (5) further, supplementing materials twice in the step (V), wherein the first supplementing is to culture for about 24 hours, adding honey with the volume of 2% of the liquid volume in the fermentation tank for first supplementing, adjusting the pH value after the first supplementing is finished, and adding molasses with the volume of 3% of the liquid volume in the fermentation tank for second supplementing after the fermentation is continued to reach a stable period.
The invention aims to provide a biochar-based compound microbial inoculant which comprises the compound microbial inoculant and biochar, wherein the compound microbial inoculant and the biochar are uniformly adsorbed on the biochar according to the ratio of 1:3, and the biochar-based compound microbial inoculant is obtained.
The invention aims to provide a preparation method of a biochar-based compound microbial inoculant, which comprises the following specific steps:
firstly, preparing biochar, namely putting crop wastes into a carbonization kiln, and carrying out high-temperature oxygen-controlled carbonization treatment to prepare biochar;
preparing a compound microbial agent by mixing, fermenting and enlarging culturing bacillus subtilis, bacillus siemens, acetobacter orientalis and saccharomyces cerevisiae;
and (III) mixing the biochar with the compound microbial agent, namely uniformly adsorbing the fermented compound microbial agent and the biochar on the biochar in a mixer according to the ratio of 1:3 to obtain the biochar-based compound microbial agent.
And (2) performing controlled pyrolysis on the biochar in the step (I) at a high temperature of 400-650 ℃ according to different materials in an anoxic environment by adopting a pyrolysis method.
A large number of pores are formed in the carbonized preform, the specific surface area of the preform is large, and the pores are large (>100 μm) of 750 to 1360m 2 The volume ratio of the pores is 51-138 m 2 (ii) a second light density of 1.5 to 1.7g/cm 3 The bulk density is 0.3 to 0.7g/cm 3 。
Further, in the step (I), the crops are firstly crushed and then are put into a carbonization kiln.
In the compound microbial agent in the step (II), the bacillus subtilis is bacillus subtilis ACCC10634, the bacillus cimicifuga is bacillus cimicifuga ACCC19948, the acetobacter orientalis is acetobacter orientalis ACCC19949, and the saccharomyces cerevisiae is saccharomyces cerevisiae ACCC 21184.
Further, the colony forming unit ratio of bacillus subtilis, bacillus siemens, acetobacter orientalis and saccharomyces cerevisiae in the composite microbial agent is 13:0.5:0.6: 0.9.
further, the fermentation medium in the compound microbial agent can be molasses with different concentrations.
Molasses is a byproduct in sugar industry, and the common brix of molasses is 80-90Bx, and contains more than 50% of sugar in water solution.
Further, in the complex microorganism bacterium solution, the culture temperature for culturing the complex microorganism bacterium solution by using a fermentation culture medium can be 20-30 ℃.
And (3) further, drying at low temperature after the step (three) to enable the moisture of the mixture to reach 15-20%.
Further, the mixture is dried at low temperature and then is crushed and sieved.
The invention aims to provide application of the compound microbial agent in preparation of a reagent for preventing and/or treating asparagus wilt.
The invention aims to provide application of the biochar-based compound microbial agent in prevention and/or treatment of asparagus wilt.
The invention aims to provide application of the compound microbial agent in preparation of a crop yield increasing promotion agent.
The invention aims to provide application of the biochar-based composite microbial inoculant in promoting crop yield increase.
Preferably, the crop is asparagus.
The charcoal-based compound microbial agent has the advantages that:
the biochar is mostly alkaline, and the ash content of the biochar contains a lot of salt-based ions, such as calcium, magnesium, potassium and the like, so that the content of hydrogen ions and exchangeable aluminum ions in soil can be reduced, and the pH value of acid soil can be increased. Meanwhile, the content of mineral nutrients in soil can be increased.
Secondly, the biochar has the characteristics of porous structure, large specific surface area and the like, and can improve the water holding capacity of soil.
The biochar has high adsorption capacity, a pore structure of the biochar and the function of storing water and nutrients, so that the biochar becomes a good inhabitation environment for microorganisms, provides protection for the microorganisms, and promotes the propagation and activity of beneficial microorganisms.
Drawings
FIG. 1 is a process diagram of the preparation of a biochar-based complex microbial inoculant.
Detailed Description
The invention is further illustrated below with reference to specific examples, which are intended to be purely exemplary of the invention and are not to be interpreted as limiting the same. The experimental procedures in the following examples are conventional unless otherwise specified. The materials, reagents and the like used in the following examples are commercially available unless otherwise specified, and several of the bacteria in the following examples were collected from the agricultural microorganism center of China Committee for culture Collection of microorganisms (abbreviated as ACCC, address: southern Town 12 of Guancun, Haishen, Beijing, institute of agricultural resources and agricultural Zones, postal code 100081) before the filing date of application, and were obtained from the agricultural microorganism center of China Committee for culture Collection of microorganisms.
Example 1
1. Preparing biochar:
putting straw, fruit shell and fruit branch into carbonization furnace, performing controlled pyrolysis at 500-600 deg.C under oxygen-deficient state to obtain biochar with large specific surface area (>100 μm) of 750 to 1360m 2 The volume ratio of the pores is 51-138 m 2 (ii) a second light density of 1.5 to 1.7g/cm 3 The bulk density is 0.3 to 0.7g/cm 3 And grinding to 100 meshes.
2. Preparation of compound microbial agent
Respectively inoculating bacillus subtilis ACCC10634, bacillus Simmer ACCC19948, acetobacter orientalis ACCC19949 and saccharomyces cerevisiae ACCC21184 in a dormant state into a test tube slant for activation, and culturing the bacillus subtilis ACCC10634 by adopting a nutrient meat juice solid culture medium at the culture temperature of 28-30 ℃ for 24-48 hours; the Bacillus Simmer ACCC19948 adopts nutrient broth solid culture medium to culture, the culture temperature is 28-30 ℃, and the culture time is 24-48 hours; culturing Acetobacter orientalis ACCC19949 in MRS solid culture medium at 30 deg.C for 24-48 hr; the Saccharomyces cerevisiae ACCC21184 is cultured in malt wort solid culture medium at 28-30 deg.C for 24-48 hr.
Inoculating activated Bacillus subtilis ACCC10634 into shake flask containing sterilized nutrient broth liquid culture medium (500mL triangular flask containing 100mL culture medium at 180rpm), inoculating Bacillus Cimeyeri ACCC19948 into shake flask containing sterilized nutrient broth liquid culture medium (500mL triangular flask containing 100mL culture medium at 180rpm), inoculating Acetobacter orientalis ACCC19949 into shake flask containing sterilized MRS liquid culture medium (500mL triangular flask containing 100mL culture medium at 180rpm), inoculating Saccharomyces cerevisiae ACCC21184 into shake flask containing sterilized malt juice culture medium (500mL triangular flask containing 100mL culture medium at 180rpm), culturing at 28-30 deg.C for 15-48 hr to obtain liquid strain of Bacillus subtilis ACCC10634, liquid strain of Bacillus Cimeyeri ACCC19948, Liquid strain of Acetobacter orientalis ACCC19949, and liquid strain of Saccharomyces cerevisiae ACCC 21184. The content of Bacillus subtilis ACCC10634 in the liquid strain is 3.2 × 10 8 cfu/ml, the content of the liquid strain of Bacillus Cimesii ACCC19948 is 2.5 × 10 8 cfu/ml, the content of Acetobacter orientalis ACCC19949 in the liquid strain of Acetobacter orientalis ACCC19949 is 5.8 × 10 8 cfu/ml, the content of Saccharomyces cerevisiae ACCC21184 in the liquid strain of Saccharomyces cerevisiae ACCC21184 is 6.1 × 10 8 cfu/ml。
And mixing the liquid strains of the bacillus subtilis ACCC10634, the liquid strains of the bacillus subtilis ACCC19948, the liquid strains of the acetobacter orientalis ACCC19949 and the liquid strains of the saccharomyces cerevisiae ACCC21184 according to the equal volume to obtain the multifunctional agricultural compound microbial agent A, wherein the colony forming unit (cuf) ratio of the bacillus subtilis ACCC10634, the bacillus occidentalis ACCC19948, the acetobacter orientalis ACCC19949 and the saccharomyces cerevisiae ACCC21184 in the multifunctional agricultural compound microbial agent A is 3.2:2.5:5.8: 6.1.
Adding 50 liters of molasses water solution with the concentration of 6 percent into a 100 liter seeding tank, sterilizing, and then inoculating the compound microbial agent A into the 100 liter seeding tank, wherein the inoculation amount is 0.8 to 1 percent, the ventilation amount is 0.5 (V/V.min) (the ratio of the volume of air introduced into a fermentation tank per minute to the volume of fermentation liquor in the fermentation tank), the rotating speed is 200rpm, and the culture is carried out for 24 hours at the temperature of 30 ℃, thus obtaining seed liquid.
Adding 500 liters of molasses water solution with the concentration of 6 percent into a 1000 liter fermentation tank, sterilizing, inoculating the seed solution into the 1000 liter fermentation tank for fermentation, wherein the inoculation amount is 10 percent (the seed solution is 10 percent of the volume of a culture medium in the fermentation tank), the aeration ratio aeration rate is 0.5 (V/V.min) (the ratio of the volume of air introduced into the fermentation tank per minute to the volume of fermentation liquid in the fermentation tank), the rotating speed is 200rpm, the pH value is controlled to be 7.0-7.2, culturing is carried out for 24 hours at 30 ℃, molasses with the volume of 2 percent of the volume of liquid in the fermentation tank is added for first time supplementation, after the first time supplementation is finished, the molasses with the volume of 3 percent of the volume of liquid in the fermentation tank is added for second time supplementation after the first time supplementation is continued for 18 hours to reach the stable period, the pH value is adjusted to 4.5-5.0 after the second time supplementation, and the other parameters are continued for fermentation for 18 hours to reach the stable period without change, and finishing the fermentation of the compound microbial agent. The content of bacillus subtilis ACCC10634 in the compound microbial agent is 13 multiplied by 10 8 cfu/ml, content of Bacillus Simmer ACCC19948 is 0.5 × 10 8 cfu/ml, 0.6 × 10 of Acetobacter orientalis ACCC19949 8 cfu/ml, Saccharomyces cerevisiae ACCC21184 content of 0.9 × 10 8 cfu/ml. In the compound microbial agent, the colony forming unit ratio of the bacillus subtilis, the bacillus siemens, the acetobacter orientalis and the saccharomyces cerevisiae is 13:0.5:0.6: 0.9.
3. Mixing of biochar and compound microbial agent
Uniformly spraying the fermented compound microbial agent on the biochar according to the ratio of 1:3, uniformly stirring, drying at low temperature until the water content reaches 15-20%, and crushing and sieving to obtain the biochar-based compound microbial agent.
Example 2
White asparagus planted in 2017 of kazao town Taicun of Shanxi Yuancheng Yongji city has asparagus wilt, and has great influence on asparagus production, thereby reducing yield. In the test land, kao township village in Youth city of Shanxi Yuancheng, the control group and the test group are adjacent and respectively belong to one mu of land, and the planted vegetables are asparagus.
Experimental groups: 10 kilograms of 200 times diluent of the biochar-based compound microbial inoculum is used for each mu 15 days before harvesting in spring and is applied along with water, and 20 kilograms of the biochar-based compound microbial inoculum is used for each mu of base fertilizer in autumn and is applied together with the base fertilizer;
control group: the same amount of base fertilizer is applied to the autumn base fertilizer and the experimental group.
The control group and the experimental group were identical for the other field management methods.
The prevention and treatment effects of the asparagus wilt are counted, the morbidity is investigated during spring collection, the number of diseased plants is recorded, and the morbidity is calculated, wherein the morbidity rate of the asparagus wilt is 100 percent of the number of the diseased plants of the asparagus wilt/the total number of the plants of the asparagus wilt.
The new bamboo shoot yield is (amount of new bamboo shoots in the experimental group-amount of new bamboo shoots in the control group)/amount of new bamboo shoots in the control group is 100%.
The yield of white asparagus is about 1800 plants per mu, and the yield is (yield of experimental group-yield of control group)/yield of control group is 100%.
TABLE 1
The experimental results show that the new yield and new stem height of the experimental group are 22.1% times and 1.7 times higher than those of the comparison group respectively, in addition, compared with the yield of the two groups, the yield of the experimental group is increased by 11.29%, the commodity characters are better, the defective quantity is obviously reduced, and in addition, the statistical results show that the white asparagus wilt is reduced by 25.1%.
Example 3
The green asparagus planted in 2018 by the agricultural science and technology (Jiangsu) Limited company of Suzuizhou Zhenbaikang in Huai 'an city, Huai' an province of Jiangsu province has asparagus wilt, and the influence on asparagus production is large, so that the yield is reduced. The land for the experiment is in a green asparagus plantation garden of Baishoukang agricultural science and technology (Jiangsu) Limited company, the control group and the experimental group are adjacent and respectively belong to one mu of land, and the planted vegetables are asparagus.
Experimental groups: 10 kilograms of 200 times diluent of the biochar-based compound microbial inoculum is used for each mu 15 days before harvesting in spring and is applied along with water, and 20 kilograms of the biochar-based compound microbial inoculum is used for each mu of base fertilizer in autumn and is applied together with the base fertilizer;
control group: the same amount of base fertilizer is applied to the autumn base fertilizer and the experimental group.
The control group and the experimental group were identical for the other field management methods.
The prevention and treatment effects of the asparagus wilt are counted, the morbidity is investigated during spring collection, the number of diseased plants is recorded, and the morbidity is calculated, wherein the morbidity rate of the asparagus wilt is 100 percent of the number of the diseased plants of the asparagus wilt/the total number of the plants of the asparagus wilt.
The new bamboo shoot yield is (the new bamboo shoot yield of the experimental group-the new bamboo shoot yield of the control group)/the new bamboo shoot yield of the control group is 100 percent.
The yield of green asparagus is about 2200 per mu (yield of experimental group-yield of control group)/yield of control group is 100%.
TABLE 2
The experimental results show that the new yield and the new stem height of the experimental group are respectively 26.7 percent times and 1.4 times higher than those of the comparison group, in addition, compared with the yield of the two groups, the yield of the experimental group is increased by 12.13 percent, the commodity characters are better, and the statistical results show that the wilt of the green asparagus is reduced by 23.2 percent.
Claims (10)
1. A preparation method of a compound microbial liquid comprises the following steps: respectively inoculating bacillus subtilis ACCC10634, bacillus Simmer ACCC19948, acetobacter orientalis ACCC19949 and saccharomyces cerevisiae ACCC21184 in a dormant state into a test tube slant containing a solid culture medium for activation; respectively inoculating the activated bacteria into shake flasks containing liquid culture media for culture; and mixing the four liquid strains obtained by culture according to the equal volume to obtain the compound microbial liquid.
2. The method according to claim 1, wherein the colony forming unit ratio of Bacillus subtilis ACCC10634, Bacillus Simmer ACCC19948, Acetobacter orientalis ACCC19949 and Saccharomyces cerevisiae ACCC21184 is 1-10:1-10: 1-10.
3. The method according to claim 1, wherein the ratio of colony forming units of Bacillus subtilis ACCC10634, Bacillus Simmer ACCC19948, Acetobacter orientalis ACCC19949 and Saccharomyces cerevisiae ACCC21184 is 3.2:2.5:5.8: 6.1.
4. The method according to claim 1, wherein the culture media are: bacillus subtilis and Bacillus Simmer are cultured in nutrient broth, Acetobacter orientalis is cultured in MRS medium, and Saccharomyces cerevisiae is cultured in malt broth.
5. A compound microbial agent, the active component of which is prepared by fermenting and expanding the compound microbial liquid prepared by the preparation method of any one of claims 1 to 4.
6. The method for preparing the complex microbial inoculant of claim 5, comprising: (1) inoculating Bacillus subtilis ACCC10634, Bacillus Simmer ACCC19948, Acetobacter orientalis ACCC19949, and Saccharomyces cerevisiae ACCC21184 in dormant state into test tube slant containing solid culture medium respectively for activation; (2) respectively inoculating the activated bacteria into shake flasks containing liquid culture media for culture; (3) mixing the four liquid strains obtained in the step (2) according to the equal volume to obtain a compound microbial solution; (4) adding molasses water solution into a seeding tank, and inoculating the compound microbial liquid prepared in the step (3) into the seeding tank after sterilization, wherein the inoculation amount is 0.8% -1%, so as to obtain a seed liquid; (5) and (3) adding molasses water solution into a fermentation tank, sterilizing, inoculating the seed solution prepared in the step (4) into the fermentation tank for fermentation, controlling the inoculation amount to be 10%, controlling the pH value to be 7.0-7.2, culturing at 26-32 ℃, supplementing materials twice during the culture process, finishing the second supplementing, adjusting the pH value to be 4.5-5.0, and completing the fermentation of the compound microbial agent after the fermentation reaches a stable period.
7. A biochar-based compound microbial agent, which comprises biochar and the compound microbial agent as claimed in claim 5 or the compound microbial agent prepared by the preparation method as claimed in claim 6.
8. The biochar-based composite microbial inoculant according to claim 7, wherein the composite microbial inoculant is uniformly adsorbed on the biochar in a ratio of 1:3 to the biochar.
9. A preparation method of a biochar-based compound microbial agent comprises the following steps: (1) preparing biochar, namely filling the crop wastes into a carbonization kiln, and performing high-temperature oxygen-controlled carbonization treatment to prepare biochar; (2) preparing a compound microbial agent by using the preparation method of claim 7; (3) and (3) mixing the biochar with the compound microbial agent, namely uniformly adsorbing the fermented compound microbial agent on the biochar in a stirrer according to a proportion.
10. Any one of the following applications:
the use of the complex microbial inoculant of claim 5 in the preparation of an agent for preventing asparagus wilt;
the use of the complex microbial inoculant of claim 5 in the preparation of an agent for promoting asparagus yield increase;
the application of the compound microbial agent prepared by the preparation method of claim 6 in preparing a reagent for preventing asparagus wilt;
the application of the compound microbial agent prepared by the preparation method of claim 6 in preparing a reagent for promoting yield increase of asparagus;
the use of the biochar-based composite microbial inoculant according to claim 7 or 8 for preventing asparagus wilt;
the application of the biochar-based composite microbial inoculant disclosed by claim 7 or 8 in promoting yield increase of asparagus;
the application of the biochar-based compound microbial agent prepared by the preparation method of claim 9 in preparing a reagent for preventing asparagus wilt;
the application of the biochar-based compound microbial agent prepared by the preparation method of claim 9 in preparing a reagent for promoting yield increase of asparagus.
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