CN103918725A - Method for preparing bactericide from bran - Google Patents
Method for preparing bactericide from bran Download PDFInfo
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- CN103918725A CN103918725A CN201410169179.3A CN201410169179A CN103918725A CN 103918725 A CN103918725 A CN 103918725A CN 201410169179 A CN201410169179 A CN 201410169179A CN 103918725 A CN103918725 A CN 103918725A
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Abstract
The invention discloses a method for preparing a bactericide from bran, which comprises the following steps: dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum to form a mixed bacterium solution; mixing the sugar solution and bacterium solution to form a mixed solution, and putting the bran into a fermenting tank layer by layer, wherein the mixed solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.
Description
Technical field
The present invention relates to a kind of preparation method of bactericide, relate in particular to a kind of method of utilizing wheat bran to prepare bactericide.
Background technology
Due to the long-term chemical pesticide that uses, serious the healthy of people that had influence on that exceed standard that various chemical pesticides are residual, the biopesticide of the friendly type of development environment becomes the main task of current Pesticide Science.
The utilization of wheat bran is very wide, also has report in the production of insecticide, as Wang Tao (application number: the production of disclosed bacillus thuringiensis,Bt solid fermentation product and using method 97109229.X); In the relevant reports such as research of the biopesticide solid fermentation condition of Tao Yugui, all narrated and utilized wheat bran as medium, added bacillus thuringiensis,Bt to carry out microorganism fermentation, produced the method for insecticide.
But the wheat bran of usining is selected suitable bacterial classification to carry out fermentation process as raw material to prepare disinfectant use in agriculture, and applicable large-scale production, and the method for production technology safety, environmental protection there is not yet report.
Summary of the invention
For addressing the above problem, the invention provides a kind of applicable large-scale production, the wheat bran that utilizes of safety and environmental protection is prepared the method for bactericide.
For achieving the above object, the technical solution adopted in the present invention is: utilize wheat bran to prepare the method for bactericide, its step is as follows:
One, batching: by sugar: the ratio of water=1: 10-20 is mixed to the water-soluble formation syrup of sugar; By two spore Zygosaccharomyces bacterium: Bayer combining yeast bacterium: the ratio of acetobacter xylinum=0-5: 0-5: 0-5 is mixed into mixed bacteria liquid;
Two, fermentation: by syrup and mixed bacteria liquid according to 80-100: 1 ratio is mixed to form mixed liquor, and wheat bran is successively put into fermentation vat, after every paving one deck, evenly spray mixed liquor to this layer moistening; Constant temperature 26-30 ℃, fermentation 15-20d;
Three, prepare bactericide: the feed liquid after fermentation is filtered, obtain zymotic fluid and solid material slag, to in gained zymotic fluid, add sodium hydroxide to regulate pH value to 6-8, again filter, must be containing the apiolin of flavonoids, the 5-nonadecyl resorcinol of alkyl-resorcin class, 5-heneicosyl resorcinol, 5-tricosyl resorcinol, the mixture of the pentosan of carbohydrate, chitosan, oligosaccharide element, amino-oligosaccharide, lentinan, Portugal's polyene sugar; Utilize this mixture to prepare botanical disinfectant use in agriculture.
Further, described botanical disinfectant use in agriculture is for wheat sharp eyespot, cucumber downy mildew, rice sheath blight disease, apple anthracnose, Phytophthora capsici disease, early blight of tomato, Alternaria alternate.
Beneficial effect of the present invention is: utilize two spore Zygosaccharomyces bacterium, Bayer combining yeast bacterium, acetobacter xylinum to mix wheat bran is fermented, the composition that is conducive to prepare botanical disinfectant use in agriculture fully discharges, and preparation technology is simple, is applicable to large-scale production; The botanical disinfectant use in agriculture safety and environmental protection of producing, non agricultural chemical residuum.
Embodiment
Embodiment 1
Utilize wheat bran to prepare the method for bactericide, its step is as follows:
One, batching: by sugar: water=1: 10 ratio is mixed to the water-soluble formation syrup of sugar; By two spore Zygosaccharomyces bacterium: Bayer combining yeast bacterium: acetobacter xylinum=0: the ratio of 2.5: 2.5 is mixed into mixed bacteria liquid;
Two, fermentation: syrup and mixed bacteria liquid are mixed to form to mixed liquor according to the ratio of 80: 1, wheat bran are successively put into fermentation vat, after every paving one deck, evenly spray mixed liquor to this layer moistening; 26 ℃ of constant temperature, fermentation 20d;
Three, prepare bactericide: the feed liquid after fermentation is filtered, obtain zymotic fluid and solid material slag, to in gained zymotic fluid, add sodium hydroxide to regulate pH value to 6, again filter, must be containing the apiolin of flavonoids, the 5-nonadecyl resorcinol of alkyl-resorcin class, 5-heneicosyl resorcinol, 5-tricosyl resorcinol, the mixture of the pentosan of carbohydrate, chitosan, oligosaccharide element, amino-oligosaccharide, lentinan, Portugal's polyene sugar; Utilize this mixture to prepare botanical disinfectant use in agriculture.
Embodiment 2
Utilize wheat bran to prepare the method for bactericide, its step is as follows:
One, batching: by sugar: water=1: 20 ratio is mixed to the water-soluble formation syrup of sugar; By two spore Zygosaccharomyces bacterium: Bayer combining yeast bacterium: acetobacter xylinum=2: the ratio of 4: 4 is mixed into mixed bacteria liquid;
Two, fermentation: syrup and mixed bacteria liquid are mixed to form to mixed liquor according to the ratio of 100: 1, wheat bran are successively put into fermentation vat, after every paving one deck, evenly spray mixed liquor to this layer moistening; 30 ℃ of constant temperature, fermentation 15d;
Three, prepare bactericide: the feed liquid after fermentation is filtered, obtain zymotic fluid and solid material slag, to in gained zymotic fluid, add sodium hydroxide to regulate pH value to 8, again filter, must be containing the apiolin of flavonoids, the 5-nonadecyl resorcinol of alkyl-resorcin class, 5-heneicosyl resorcinol, 5-tricosyl resorcinol, the mixture of the pentosan of carbohydrate, chitosan, oligosaccharide element, amino-oligosaccharide, lentinan, Portugal's polyene sugar; Utilize this mixture to prepare botanical disinfectant use in agriculture.
Embodiment 3
Utilize wheat bran to prepare the method for bactericide, its step is as follows:
One, batching: by sugar: water=1: 15 ratio is mixed to the water-soluble formation syrup of sugar; By two spore Zygosaccharomyces bacterium: Bayer combining yeast bacterium: acetobacter xylinum=3: the ratio of 5: 5 is mixed into mixed bacteria liquid;
Two, fermentation: syrup and mixed bacteria liquid are mixed to form to mixed liquor according to the ratio of 90: 1, wheat bran are successively put into fermentation vat, after every paving one deck, evenly spray mixed liquor to this layer moistening; 28 ℃ of constant temperature, fermentation 18d;
Three, prepare bactericide: the feed liquid after fermentation is filtered, obtain zymotic fluid and solid material slag, to in gained zymotic fluid, add sodium hydroxide to regulate pH value to 7, again filter, must be containing the apiolin of flavonoids, the 5-nonadecyl resorcinol of alkyl-resorcin class, 5-heneicosyl resorcinol, 5-tricosyl resorcinol, the mixture of the pentosan of carbohydrate, chitosan, oligosaccharide element, amino-oligosaccharide, lentinan, Portugal's polyene sugar; Utilize this mixture to prepare botanical disinfectant use in agriculture.
After above-mentioned production technology, the botanical disinfectant use in agriculture of preparation is respectively 82.6%, 85.3%, 97.8%, 79.4%, 89.7%, 77.2%, 78.6%, 81.4% to the inhibiting rate of the fungoid phytopathogens such as wheat sharp eyespot, cucumber downy mildew, rice sheath blight disease, apple anthracnose, Phytophthora capsici disease, early blight of tomato, Alternaria alternate when concentration 1000ug/ml.
The explanation of above embodiment, only for understanding principle of the present invention, is not understood to limitation of the present invention.
Claims (2)
1. utilize wheat bran to prepare the method for bactericide, it is characterized in that, step is as follows:
One, batching: by sugar: the ratio of water=1: 10-20 is mixed to the water-soluble formation syrup of sugar; By two spore Zygosaccharomyces bacterium: Bayer combining yeast bacterium: the ratio of acetobacter xylinum=0-5: 0-5: 0-5 is mixed into mixed bacteria liquid;
Two, fermentation: by syrup and mixed bacteria liquid according to 80-100: 1 ratio is mixed to form mixed liquor, and wheat bran is successively put into fermentation vat, after every paving one deck, evenly spray mixed liquor to this layer moistening; Constant temperature 26-30 ℃, fermentation 15-20d;
Three, prepare bactericide: the feed liquid after fermentation is filtered, obtain zymotic fluid and solid material slag, to in gained zymotic fluid, add sodium hydroxide to regulate pH value to 6-8, again filter, must be containing the apiolin of flavonoids, the 5-nonadecyl resorcinol of alkyl-resorcin class, 5-heneicosyl resorcinol, 5-tricosyl resorcinol, the mixture of the pentosan of carbohydrate, chitosan, oligosaccharide element, amino-oligosaccharide, lentinan, Portugal's polyene sugar; Utilize this mixture to prepare botanical disinfectant use in agriculture.
2. the method for utilizing wheat bran to prepare bactericide according to claim 1, is characterized in that: described botanical disinfectant use in agriculture is for wheat sharp eyespot, cucumber downy mildew, rice sheath blight disease, apple anthracnose, Phytophthora capsici disease, early blight of tomato, Alternaria alternate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410169179.3A CN103918725B (en) | 2014-04-18 | 2014-04-18 | Utilize the method that wheat bran prepares antibacterial |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410169179.3A CN103918725B (en) | 2014-04-18 | 2014-04-18 | Utilize the method that wheat bran prepares antibacterial |
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| CN103918725A true CN103918725A (en) | 2014-07-16 |
| CN103918725B CN103918725B (en) | 2016-06-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282067A (en) * | 2016-08-24 | 2017-01-04 | 李丽丽 | Multifunctional agricultural complex micro organism fungicide and microbial ecological agent and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100658A (en) * | 1986-01-21 | 1987-10-07 | 云南省玉溪市北山林场 | The solid culture method of white muscardine fungi |
| US20060052246A1 (en) * | 2003-03-27 | 2006-03-09 | Council Of Scientific And Industrial Research | Plant growth stimulator |
| CN101348813A (en) * | 2008-07-18 | 2009-01-21 | 天津科技大学 | Preparation of bacteria cellulose tube having bacteriostasis property |
| CN103360182A (en) * | 2012-03-27 | 2013-10-23 | 苏文琳 | Natural soil conditioner |
-
2014
- 2014-04-18 CN CN201410169179.3A patent/CN103918725B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100658A (en) * | 1986-01-21 | 1987-10-07 | 云南省玉溪市北山林场 | The solid culture method of white muscardine fungi |
| US20060052246A1 (en) * | 2003-03-27 | 2006-03-09 | Council Of Scientific And Industrial Research | Plant growth stimulator |
| CN101348813A (en) * | 2008-07-18 | 2009-01-21 | 天津科技大学 | Preparation of bacteria cellulose tube having bacteriostasis property |
| CN103360182A (en) * | 2012-03-27 | 2013-10-23 | 苏文琳 | Natural soil conditioner |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282067A (en) * | 2016-08-24 | 2017-01-04 | 李丽丽 | Multifunctional agricultural complex micro organism fungicide and microbial ecological agent and application |
| CN106282067B (en) * | 2016-08-24 | 2019-10-11 | 李丽丽 | Multifunctional agricultural complex micro organism fungicide and probiotics and application |
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| CN103918725B (en) | 2016-06-29 |
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