CN112481161A - Microbial agent for promoting wheat growth and wheat breeding method thereof - Google Patents

Microbial agent for promoting wheat growth and wheat breeding method thereof Download PDF

Info

Publication number
CN112481161A
CN112481161A CN202011364492.4A CN202011364492A CN112481161A CN 112481161 A CN112481161 A CN 112481161A CN 202011364492 A CN202011364492 A CN 202011364492A CN 112481161 A CN112481161 A CN 112481161A
Authority
CN
China
Prior art keywords
burkholderia
pseudomonas
wheat
microbial agent
microbial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202011364492.4A
Other languages
Chinese (zh)
Inventor
李华一
杨效帆
罗钰彬
沈家葆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Lihao Biological Agriculture Co ltd
Original Assignee
Guangdong Lihao Biological Agriculture Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Lihao Biological Agriculture Co ltd filed Critical Guangdong Lihao Biological Agriculture Co ltd
Priority to CN202011364492.4A priority Critical patent/CN112481161A/en
Publication of CN112481161A publication Critical patent/CN112481161A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Genetics & Genomics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a microbial agent for promoting wheat growth, which consists of fermentation liquor of pseudomonas and burkholderia in a weight ratio of 1: 1-5. The microbial agent disclosed by the invention can be fixedly planted in wheat bodies, and also has the effects of improving the alkali resistance of wheat, shortening the seedling emergence time of wheat and improving the seedling emergence rate.

Description

Microbial agent for promoting wheat growth and wheat breeding method thereof
Technical Field
The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a microbial agent for promoting wheat growth and a wheat breeding method thereof.
Background
Wheat is the third major food crop in China, however, the wheat planting management in China at present highly depends on chemical fertilizers, pesticides and plant growth regulators, and the function of beneficial bacteria endophytes is ignored. The inoculation of the seeds is to attach microorganisms to the surfaces of the seeds by soaking, smearing, painting, adsorbing and adhering, and the mode can prevent and control plant diseases from beginning. However, this method has its own drawbacks, such as: the number of microorganisms on the epidermis is limited, and the functional effects that some seeds can play are greatly reduced because the seeds are too small or are not easy to attach; the inoculated microorganisms often compete with indigenous microorganisms, compete for the energy source of carbon source nitrogen, compete on ecological niches, compete on living space, and have stronger competitive capacity, so that the inoculated microorganisms can be reduced and disappear; exogenous microorganisms are influenced by agricultural operation, the agricultural fertilizer is used frequently, the fertilizer can destroy the microbial environment of soil, and the pesticide can kill the exogenous microorganisms of the soil.
Currently, wheat varieties on the market are the result of artificial domestication through the mode for thousands of years, and the domestication of wheat can cause reduction of beneficial strains and quantity of endophytes. In the process of domesticating and breeding wheat, people pay attention to the characteristics of high yield, disease resistance, strong adaptability, high nutrient efficiency, water conservation and stress resistance, neglect the endogenous beneficial bacteria of other wheat and plants of related species thereof and lose some potential excellent characters. Because wheat varieties lack endogenous beneficial bacteria and are increasingly weak in stress resistance and pest resistance, people still rely on pesticide, bactericide and herbicide and excessive chemical fertilizer to achieve the effects of preventing insects and pests. Therefore, the search for a green approach to reduce the abuse of phytohormones in fertilizers and pesticides is a problem to be solved urgently by those skilled in the art.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for planting a compound microbial agent in wheat to improve the self performance of wheat, shorten the time of wheat emergence and improve the rate of emergence.
In order to achieve the purpose, the invention adopts the following technical scheme:
the composite microbial agent consists of fermentation liquor of pseudomonas and burkholderia, and the weight ratio of the pseudomonas to the burkholderia is 1: 1-5.
The pseudomonas has the function of hydroxide bacteria, and can absorb H in soil and environment2The Burkholderia is used as a rhizosphere microorganism for biodegradation, biological control and plant growth promotion in agriculture, and the inventor finds that the combination of the Burkholderia and the rhizosphere microorganism can shorten the time of wheat emergence and improve the rate of emergence.
Preferably, the weight ratio of the pseudomonas to the burkholderia is 1: 3. The inventors have found that the effect is good when the ratio of the weight parts of Pseudomonas to Burkholderia is in the range of 1:1 to 5, and the effect is best when the ratio of the weight parts is 1: 3.
Preferably, the Pseudomonas may be Pseudomonas fluorescens (Pseudomonas fluorescens) or Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) or Pseudomonas stutzeri (Pseudomonas stutzeri). More preferably, the Pseudomonas is Pseudomonas fluorescens (Pseudomonas fluorescens).
Preferably, the Burkholderia may be Burkholderia nodorum (Burkholderia turberum) or Burkholderia saccharose (Burkholderia saccharoi) or Burkholderia grimmiae. More preferably, the Burkholderia is Burkholderia saccharolytica (Burkholderia saccharoi).
The invention also provides a wheat breeding method, which comprises the following steps: the microbial agent of the present invention is sprayed on the ear of wheat at the flowering stage.
The invention also provides application of the microbial agent in improving crop yield.
The invention also provides application of the microbial agent in preparation of a preparation for improving saline-alkali resistance of crops.
The invention has the beneficial effects that: the microbial agent disclosed by the invention can be fixedly planted in wheat bodies, and also has the effects of improving the alkali resistance of wheat, shortening the seedling emergence time of wheat and improving the seedling emergence rate.
Detailed Description
In order to show technical solutions, purposes and advantages of the present invention more concisely and clearly, the technical solutions of the present invention are described in detail below with reference to specific embodiments. The various microorganisms in the invention can be purchased in the market or can be obtained from China general microbiological culture Collection center, and the preparation method of the culture solution of various microorganisms adopts the corresponding standard culture medium and culture solution according to the conventional culture method.
Example 1 preparation of microbial Agents of the invention
(1) Cultivation of the Strain
A, pseudomonas: inoculating pseudomonas fluorescens into NYBD liquid culture medium, performing shake culture at 28 ℃ for 48h to obtain bacterial suspension, and performing amplification culture until the concentration of thalli is not less than 106mL, secondary cultures were obtained. The 1L culture medium contains 8g of beef extract, 5g of yeast extract powder and 10g of glucose.
B, culture of the Burkholderia: inoculating Burkholderia saccharolytica into liquid culture medium, culturing at 28 deg.C for 48h to obtain bacterial suspension, and performing amplification culture until the thallus concentration is not less than 106mL, secondary cultures were obtained. The 1L medium contained 55g of glucose, 15g of magnesium sulfate heptahydrate, 15g of ammonium sulfate, 20g of calcium carbonate, 0.5g of potassium dihydrogen phosphate, 10g of peptone and 5g of sodium chloride.
C, mixing the fermentation liquors cultured in the steps A and B according to the weight ratio of 1:3, and detecting that the viable count is 105-1010CFU/ml。
Example 2 method for colonization of wheat by microbial agent
The embodiment provides a method for planting a compound microbial agent in wheat, which comprises the following specific steps:
the microbial inoculum prepared in the example 1 is filled in a sprayer, the microbial inoculum is sprayed on the ear part of Zheng wheat 7698, and the ear is sprayed on the naked tissue of one third of the ear for five days continuously; the bacterial liquid is 680 microgram/microliter-3500 microgram/microliter per spikelet. The bacteria sprayed on the leaves, the stems, the base and the soil are timely and clear, and interference is avoided. The part below the wheat ear is shielded, so that the thallus is prevented from falling on leaves, stems, base parts and soil of wheat, and the interference and influence of spraying on other wheat plants are prevented.
The wheat is divided into a control group and a treatment group, the control group does not apply the microbial inoculum of the example 1, the treatment group sprays the microbial inoculum of the example 1 on the ear of the wheat according to the method, and the microbial colonization condition in the microbial inoculum is observed.
Sampling the root system in the seedling emergence stage and the jointing stage of the wheat, obtaining 16sDNA for determination, and determining the bacterial sample of the root system. It was found that the control group was not colonized by Pseudomonas fluorescens and Burkholderia saccharolytica during the emergence and elongation stages of wheat, and the treatment group was colonized by Pseudomonas fluorescens and Burkholderia saccharolytica in large amounts.
And (3) when the wheat is about to enter the mature period, taking the green grains, and grinding the grains to obtain 16sDNA for determination. No pseudomonas fluorescens and burkholderia saccharolytica were found in the control group, but 97% of the wheat grain in the treated group was colonized by pseudomonas fluorescens and burkholderia saccharose.
Example 3 Effect of the microbial Agents of the invention on wheat Germination
Wheat grains obtained from the control group and the treatment group in the example 2 were dehydrated in an oven, and then another land which was not treated with the bacterial powder was selected for planting, and during the planting period, 10 kg/mu of nitrogen fertilizer, 2 kg of phosphate fertilizer, 2 kg/mu of potassium fertilizer, 1 kg/mu of zinc sulfate and 0.2 kg/mu of borax were applied. And observing the germination condition of the seeds of the control group and the treated group. The results are shown in table 1:
TABLE 1
Group (n 20) 3 days 4 days 5 days 6 days
Control group 0 6 2 12
Treatment group 15 4 1 0
As can be seen from Table 1, compared with the control group, the germination time of the wheat in the treatment group is 75% in 3 days, while most of the wheat in the control group germinates after 6 days, which shows that the microbial inoculum of the invention can greatly shorten the germination time and improve the germination rate.
Example 4 Effect of the microbial Agents of the invention on saline tolerance of wheat
The wheat grains obtained from the control group and the treatment group in the example 2 were dehydrated in an oven, then another piece of saline-alkali soil was selected for planting, all the planting conditions were consistent, and the germination conditions of the seeds of the control group and the treatment group were observed. The results are shown in table 2:
table 2:
Figure BDA0002805030850000041
Figure BDA0002805030850000051
as can be seen from Table 2, compared with the control group, 14 seeds in the treatment group of the saline-alkali soil germinated, the germination rate of the seeds reached 70%, while the germination rate of the control group was only 25%, so that the strains of the microbial inoculum of the invention planted in the seeds, the saline-alkali resistance of wheat was improved, and the performance was improved.
Example 5
The only difference between the microbial agent in this example and example 1 is that the weight ratio of pseudomonas fluorescens to burkholderia saccharolytica in this example is 1: 1.
Example 6
The only difference between the microbial agent in this example and example 1 is that the weight ratio of pseudomonas fluorescens to burkholderia saccharolytica in this example is 1: 2.
Example 7
The only difference between the microbial agent in this example and example 1 is that the weight ratio of pseudomonas fluorescens to burkholderia saccharolytica in this example is 1: 4.
Example 8
The only difference between the microbial agent in this example and example 1 is that the weight ratio of pseudomonas fluorescens to burkholderia saccharolytica in this example is 1: 5.
Example 8
The only difference between the microbial agent in this example and example 1 is that the pseudomonas in this example is pseudomonas plecoglossus.
Example 9
The only difference between the microbial agent in this example and example 1 is that the pseudomonas in this example is pseudomonas stutzeri.
Example 10
The only difference between the microbial agents in this example and example 1 is that the burkholderia nodorum in this example is burkholderia nodorum.
Example 11
The only difference between the microbial agent in this example and example 1 is that burkholderia in this example is burkholderia.
Comparative example 1
The only difference between the microbial agents in this example and example 1 is that comparative example 1 does not contain Pseudomonas bacteria.
Comparative example 2
The only difference between the microbial agents in this example and example 1 is that no Burkholderia bacteria were contained in comparative example 2.
Comparative example 3
The only difference between the microbial agent in this example and example 1 is that the pseudomonas in comparative example 3 is rhodomonas capsulata.
Comparative example 4
The only difference between the microbial agents in this example and example 1 is that the pseudomonas in comparative example 4 is pseudomonas sphaerica.
Comparative example 5
The only difference between the microbial agents in this example and example 1 is that the burkholderia cepacia in comparative example 5 is burkholderia cepacia.
Comparative example 6
The only difference between the microbial agent in this example and example 1 is that the burkholderia gonorrhoeae in comparative example 6 is burkholderia cerealis.
Comparative example 7
The only difference between the microbial agent in this example and example 1 is that the weight part ratio of pseudomonas fluorescens to burkholderia saccharolytica in comparative example 7 is 1: 0.5.
Comparative example 8
The only difference between the microbial agent in this example and example 1 is that the weight ratio of pseudomonas fluorescens to burkholderia saccharolytica in comparative example 7 is 1: 6.
The microbial agents of examples 5-11 and comparative examples 1-8 were treated on wheat seeds according to the method of example 2, and the effect on wheat germination was compared, and the results are shown in table 3:
TABLE 3
Figure BDA0002805030850000071
Figure BDA0002805030850000081
As can be seen from Table 3, the emergence time of the wheat seeds treated by the microbial agents of examples 1 and 5-11 is greatly shortened, even shortened to half the time of the control group, which is beneficial to shortening the harvest time of the whole wheat. Both comparative examples 1 and 2 lack one of them, and thus it was concluded that Pseudomonas and Burkholderia in the microbial agent of the present invention had a synergistic effect, and the effect of the present invention could not be achieved by either of them. The comparative examples 3-6 are replaced by other bacteria, and the effect of the invention is not achieved, so that not all the combination of pseudomonas and burkholderia can generate synergistic effect to shorten the germination period of wheat seeds; in addition, in comparative examples 7 and 8, the effect of advancing wheat germination was not obtained even when the ratio of the parts by weight of Pseudomonas and Burkholderia was exceeded.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (9)

1. The compound microbial agent is characterized by comprising fermentation liquor of pseudomonas and burkholderia, wherein the weight ratio of the pseudomonas to the burkholderia is 1: 1-5.
2. The complex microbial inoculant according to claim 1, wherein the weight ratio of pseudomonas to burkholderia is 1: 3.
3. The complex microbial inoculant according to claim 1, wherein the Pseudomonas bacteria can be Pseudomonas fluorescens (Pseudomonas fluorescens) or Pseudomonas plecoglossus (Pseudomonas plecoglossicida) or Pseudomonas stutzeri (Pseudomonas stutzeri).
4. The complex microbial inoculant according to claim 1, wherein the Pseudomonas is Pseudomonas fluorescens (Pseudomonas fluorescens).
5. The complex microbial inoculant according to claim 1, wherein the Burkholderia can be Burkholderia nodorum (Burkholderia turberum) or Burkholderia saccharoi (Burkholderia sacchara) or Burkholderia grimmiae (Burkholderia grimmiae).
6. The complex microbial inoculant according to claim 1, wherein the Burkholderia saccharose (Burkholderia saccharo) is Burkholderia saccharose.
7. A method for breeding wheat, which is characterized by comprising the following steps: the microbial preparation according to claim 1 is sprayed onto the ear of wheat during flowering.
8. Use of the microbial inoculant according to claim 1 for the preparation of a preparation for reducing the germination of crops.
9. The use of the microbial inoculant of claim 1 in the preparation of a formulation for enhancing saline and alkaline tolerance in crops.
CN202011364492.4A 2020-11-27 2020-11-27 Microbial agent for promoting wheat growth and wheat breeding method thereof Pending CN112481161A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011364492.4A CN112481161A (en) 2020-11-27 2020-11-27 Microbial agent for promoting wheat growth and wheat breeding method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011364492.4A CN112481161A (en) 2020-11-27 2020-11-27 Microbial agent for promoting wheat growth and wheat breeding method thereof

Publications (1)

Publication Number Publication Date
CN112481161A true CN112481161A (en) 2021-03-12

Family

ID=74936654

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011364492.4A Pending CN112481161A (en) 2020-11-27 2020-11-27 Microbial agent for promoting wheat growth and wheat breeding method thereof

Country Status (1)

Country Link
CN (1) CN112481161A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113439656A (en) * 2021-06-28 2021-09-28 广东丽豪生物农业有限公司 Microbial inoculation and wheat breeding method
CN113558066A (en) * 2021-06-28 2021-10-29 广东丽豪生物农业有限公司 Bacterial liquid for wheat breeding and wheat flowering phase breeding method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1203945A (en) * 1998-07-03 1999-01-06 中国农业科学院原子能利用研究所 Azotification Se's pseudomonas denitrificas and its mycoderm culture method
CN102787090A (en) * 2012-08-07 2012-11-21 哈尔滨师范大学 Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5
US20160143295A1 (en) * 2013-06-10 2016-05-26 B.G. Negev Technologies And Applications Ltd. Plant growth-promoting microorganisms and methods of use thereof
CN108290799A (en) * 2015-06-05 2018-07-17 可持续有机解决方案私人有限公司 Microbial inoculant, fertilizer composition, growth substrate and the method for enhancing plant growth
CN110699290A (en) * 2019-11-01 2020-01-17 华东师范大学 Stable burkholderia, microbial inoculum and preparation method and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1203945A (en) * 1998-07-03 1999-01-06 中国农业科学院原子能利用研究所 Azotification Se's pseudomonas denitrificas and its mycoderm culture method
CN102787090A (en) * 2012-08-07 2012-11-21 哈尔滨师范大学 Alfalfa root growth-promoting rhizobacteria MJM-5 and application of alfalfa root growth-promoting rhizobacteria MJM-5
US20160143295A1 (en) * 2013-06-10 2016-05-26 B.G. Negev Technologies And Applications Ltd. Plant growth-promoting microorganisms and methods of use thereof
CN108290799A (en) * 2015-06-05 2018-07-17 可持续有机解决方案私人有限公司 Microbial inoculant, fertilizer composition, growth substrate and the method for enhancing plant growth
CN110699290A (en) * 2019-11-01 2020-01-17 华东师范大学 Stable burkholderia, microbial inoculum and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KIM JI YOUN等: "Plant Growth Promoting and Disease Controlling Activities of Pseudomonas geniculata ANG3, Exiguobacterium acetylicum ANG40 and Burkholderia stabilis ANG51 Isolated from Soil", 《MICROBIOLOGY AND BIOTECHNOLOGY LETTERS》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113439656A (en) * 2021-06-28 2021-09-28 广东丽豪生物农业有限公司 Microbial inoculation and wheat breeding method
CN113558066A (en) * 2021-06-28 2021-10-29 广东丽豪生物农业有限公司 Bacterial liquid for wheat breeding and wheat flowering phase breeding method

Similar Documents

Publication Publication Date Title
CN112501061B (en) Microbial agent for wheat planting
CN101786914B (en) Microorganism fertilizer for promoting rice production, preparation method and application thereof
CN112481159B (en) Microbial agent and application thereof
CN110616172B (en) Bacillus with crucifer clubroot prevention and treatment function and preparation method and application thereof
CN108277177B (en) Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application
CN110938559B (en) Compound microbial agent for preventing and treating soil-borne diseases, preparation method and application thereof in summer corn cultivation
CN110862939A (en) Special microbial agent for apple trees and preparation method thereof
CN112746024A (en) Trichoderma composite microbial agent and bio-organic fertilizer prepared from same
CN112481161A (en) Microbial agent for promoting wheat growth and wheat breeding method thereof
CN110343628B (en) Microbial agent containing candida ethanolate and application thereof
CN111763651A (en) Special microbial agent and bacterial fertilizer for potatoes and application of microbial agent and bacterial fertilizer
CN109136144B (en) Special compound biological agent for ginseng and production and preparation method thereof
CN106342898A (en) Compound microorganism preparation against sclerotinia rot of colza and preparing method thereof
CN108293480B (en) Method for preventing and treating tomato gray mold by using biocontrol microbial inoculum
CN114600911B (en) Compound microbial agent for preventing and treating root rot of fruits and vegetables as well as preparation method and application thereof
CN114672431B (en) Bacillus composite microbial inoculant for preventing and treating bacterial wilt and application thereof
CN109957535A (en) Simple bacillus, microbial bacterial agent, bio-fertilizer and the application prepared using it
CN115557815A (en) Ginger compound microbial fertilizer capable of solving continuous cropping obstacle and preparation method thereof
CN113881605A (en) Compound microbial agent for preventing potato scab and preparation method thereof
CN109716974B (en) Verticillium wilt prevention and control method for cotton verticillium wilt severe disease field
CN110627566B (en) Bio-organic fertilizer with clubroot disease prevention and treatment function and preparation method and application thereof
CN113897316A (en) Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application of functional melon and fruit seedling biological matrix
CN109456900A (en) A kind of composite biological agent and application thereof
CN108203703B (en) Biocontrol microbial inoculum and application thereof in preventing and treating gray mold
CN113004080A (en) Composite microbial agent for promoting photosynthesis of plant leaf surfaces and preparation and use methods thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20210312