CN113367161A - Compound microbial agent for effectively preventing and treating soil-borne diseases and application thereof - Google Patents
Compound microbial agent for effectively preventing and treating soil-borne diseases and application thereof Download PDFInfo
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention discloses a compound microbial agent for effectively preventing and treating soil-borne diseases, relates to the technical field of microorganisms, and mainly aims to solve the problem of single prevention and treatment effect of the existing microbial agent; the method comprises the following raw materials: the composite bacterial agent disclosed by the embodiment of the invention is used by matching multiple strains, so that the problems that a single strain only can play a single role, the control effect is single, the required bacterial amount is large are solved, the biocompatibility is good, the comprehensive effects of the strains are achieved, the strains are mutually beneficial, the soil-borne diseases are effectively controlled, and the using amount of pesticides can be reduced; the effective components of the traditional Chinese medicine can be utilized by adding the traditional Chinese medicine composition, the insecticidal and bactericidal effects are further achieved, the effect of preventing and treating soil-borne diseases can be further improved by adding the modified black soil and the modified sodium lignosulfonate, and the synergistic effect of the modified black soil and the modified sodium lignosulfonate is achieved, so that the traditional Chinese medicine composition has a wide development prospect.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a compound microbial agent for effectively preventing and treating soil-borne diseases and application thereof.
Background
The microbial agent is a preparation prepared from target microorganisms;
the microbial agent is widely applied in agriculture, can also be used for preventing and treating some soil-borne diseases, and can improve the yield of agricultural products, improve the quality of the agricultural products, reduce the using amount of chemical fertilizers, reduce the cost, improve the soil and protect the ecological environment by reasonable use.
However, the existing microbial agent is generally prepared by a single microbial agent, and has the advantages of large dosage, single control effect and not very obvious effect.
Disclosure of Invention
The embodiment of the invention aims to provide a compound microbial agent for effectively preventing and treating soil-borne diseases and application thereof, so as to solve the problems.
In order to achieve the above purpose, the embodiments of the present invention provide the following technical solutions:
a compound microbial agent for effectively preventing and treating soil-borne diseases comprises the following raw materials in parts by weight: 50-70 parts of compound bacteria, 4-8 parts of a traditional Chinese medicine composition, 10-14 parts of subcritical hydrolysis products, 12-18 parts of modified black soil, 2-6 parts of humic acid, 1-3 parts of wetting agent, 3-5 parts of seaweed, 2-4 parts of modified sodium lignosulfonate and 8-12 parts of cane sugar.
On the basis of the above technical solutions, the embodiments of the present invention further provide the following optional technical solutions:
in one alternative: the feed comprises the following raw materials in parts by weight: 55-65 parts of compound bacteria, 5-7 parts of a traditional Chinese medicine composition, 11-13 parts of subcritical hydrolysis products, 13-17 parts of modified black soil, 3-5 parts of humic acid, 1.5-2.5 parts of a wetting agent, 3.5-4.5 parts of seaweed, 2.5-3.5 parts of modified sodium lignosulfonate and 9-11 parts of cane sugar.
In one alternative: the feed comprises the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar.
In one alternative: the compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
In one alternative: the mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1-2: 2-3.
In one alternative: the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 1-3 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
In one alternative: the preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
In one alternative: the preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 30-50mm, and carrying out surface modification on the black soil by adopting a modifier under a weakly acidic environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.01-0.03.
In one alternative: the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 2-3.
In one alternative: the pretreatment of the black soil comprises the following steps: exposing black soil in the sun for 2-3 hours, then adding taro powder 0.1-0.3 time of the weight of black soil blocks, locust corpses 0.1-0.2 time of the weight of black soil blocks, berberine 0.005-0.015 time of the weight of black soil blocks and clear water 1-2 times of the weight of black soil blocks at the temperature of 50-60 ℃, stirring for 30-40 minutes, putting the mixture into a drying oven, and drying the mixture for 1-2 hours at the temperature of 40-50 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
In one alternative: the preparation method of the modified sodium lignosulfonate comprises the following steps: adding 30-50 parts of sodium lignosulfonate into 300 parts of water 200-plus, stirring for 30-50min at the rotation speed of 600 revolutions per minute of 500-plus, adjusting the pH to 4-6 by using 0.01-0.05mol/L hydrochloric acid, adding 0.05-0.08 part of beta-glucosidase, stirring for 60-100min at the rotation speed of 500 revolutions per minute of 400-plus at the temperature of 40-60 ℃, adding 0.05-0.25 part of horseradish peroxidase, adding 0.1-0.5 part of hydrogen peroxide with the mass concentration of 20-30%, stirring for 1-3h at the rotation speed of 500 revolutions per minute of 400-plus at the temperature of 50-70 ℃, removing the solvent water by distillation, adding the obtained product into 400 parts of ethanol 300-plus, stirring for 15-25min at the rotation speed of 400 revolutions per minute of 300-plus, and then centrifuging for 20-40min at the rotation speed of 10000-plus, and drying the precipitate obtained by centrifugation at 55-75 ℃ for 4-8h to obtain the modified sodium lignosulfonate.
The preparation method of the compound microbial agent for effectively preventing and treating soil-borne diseases comprises the following steps:
1) weighing the raw materials according to the proportion;
2) uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture;
3) adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g.
The application of the compound microbial agent for effectively preventing and treating soil-borne diseases is characterized in that the application method comprises the following steps: the microbial inoculum is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 30-40 kg/mu.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
the composite microbial inoculum of the embodiment of the invention solves the problems that a single strain only can play a single role, the prevention and control effect is single, the required bacterial amount is large, the biocompatibility is good, the strains have comprehensive effects and are mutually enhanced, the soil-borne diseases are effectively prevented and controlled, partial pesticides can be effectively replaced, the use amount of the pesticides is reduced, and the development requirement of green agriculture is met; the effective components of the traditional Chinese medicine can be utilized by adding the traditional Chinese medicine composition, the insecticidal and bactericidal effects are further achieved, the effect of preventing and treating soil-borne diseases can be further improved by adding the modified black soil and the modified sodium lignosulfonate, and the synergistic effect of the modified black soil and the modified sodium lignosulfonate is achieved, so that the traditional Chinese medicine composition has a wide development prospect.
Detailed Description
The present invention will be described in detail with reference to the following examples, which are provided for illustrative purposes only and are not intended to limit the scope of the present invention. Any obvious modifications or variations can be made to the present invention without departing from the spirit or scope of the present invention.
The composite microbial inoculum of the embodiment of the invention solves the problems that a single strain only has a single function, the control effect is single, the required bacterial amount is large, the biocompatibility is good, the comprehensive functions of all strains are mutually enhanced, and the soil-borne diseases are effectively controlled by using the multiple strains in a matching way.
Example 1
Weighing the following raw materials in parts by weight: 50 parts of compound bacteria, 4 parts of a traditional Chinese medicine composition, 10 parts of subcritical hydrolysis products, 12 parts of modified black soil, 2 parts of humic acid, 1 part of wetting agent, 3 parts of seaweed, 2 parts of modified sodium lignosulfonate and 8 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 30 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1:2.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 1 time; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 30mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.01.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1:2.
The pretreatment of the black soil comprises the following steps: the pretreatment of the black soil comprises the following steps: exposing the black soil in the sun for 2-3 hours, then adding taro powder 0.1 time of the weight of the black soil, locust corpses 0.1 time of the weight of the black soil, berberine 0.005 time of the weight of the black soil and clear water 1 time of the black soil at the temperature of 50 ℃, stirring for 30 minutes, putting the mixture into a baking oven, and drying for 1 hour at the temperature of 40 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 30 parts of sodium lignosulfonate into 200 parts of water, stirring for 30min at the rotating speed of 500 revolutions/min, adjusting the pH to 4 by using 0.01mol/L hydrochloric acid, adding 0.05 part of beta-glucosidase, stirring for 60min at the rotating speed of 400 revolutions/min at 40 ℃, adding 0.05 part of horseradish peroxidase, adding 0.1 part of hydrogen peroxide with the mass concentration of 20%, stirring for 1h at the rotating speed of 400 revolutions/min at 50 ℃, removing solvent water by distillation, adding the obtained product into 300 parts of ethanol, stirring for 15min at the rotating speed of 300 revolutions/min at 25 ℃, centrifuging for 20min at the rotating speed of 8000 revolutions/min, and drying the centrifuged precipitate for 4h at the temperature of 55 ℃ to obtain the modified sodium lignosulfonate.
Example 2
Weighing the following raw materials in parts by weight: 55 parts of compound bacteria, 5 parts of a traditional Chinese medicine composition, 11 parts of subcritical hydrolysis products, 13 parts of modified black soil, 3 parts of humic acid, 1.5 parts of wetting agent, 3.5 parts of seaweed, 2.5 parts of modified sodium lignosulfonate and 9 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 30 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1:2.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 1 time; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 30mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.01.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1:2.
The pretreatment of the black soil comprises the following steps: exposing black soil in the sun for 2 hours, then adding taro powder which is 0.1 time of the weight of the black soil block, locust corpses which are 0.1 time of the weight of the black soil block, berberine which is 0.005 time of the weight of the black soil block and clear water which is 1 time of the black soil block at the temperature of 50 ℃, stirring for 30 minutes, putting the mixture into a drying oven, and drying for 1 hour at the temperature of 40 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 30 parts of sodium lignosulfonate into 200 parts of water, stirring for 30min at the rotating speed of 500 revolutions/min, adjusting the pH to 4 by using 0.01mol/L hydrochloric acid, adding 0.05 part of beta-glucosidase, stirring for 60min at the rotating speed of 400 revolutions/min at 40 ℃, adding 0.05 part of horseradish peroxidase, adding 0.1 part of hydrogen peroxide with the mass concentration of 20%, stirring for 1h at the rotating speed of 400 revolutions/min at 50 ℃, removing solvent water by distillation, adding the obtained product into 300 parts of ethanol, stirring for 15min at the rotating speed of 300 revolutions/min at 25 ℃, centrifuging for 20min at the rotating speed of 8000 revolutions/min, and drying the centrifuged precipitate for 4h at the temperature of 55 ℃ to obtain the modified sodium lignosulfonate.
Example 3
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 35 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 40mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.02.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 2.5.
The pretreatment of the black soil comprises the following steps: exposing the black soil in the sun for 2.5 hours, then adding taro powder which is 0.2 time of the weight of the black soil block, locust corpses which are 0.15 time of the weight of the black soil block, berberine which is 0.01 time of the weight of the black soil block and clear water which is 1.5 times of the weight of the black soil block at the temperature of 55 ℃, stirring for 35 minutes, putting the mixture into an oven, and drying the mixture for 1.5 hours at the temperature of 45 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 40 parts of sodium lignin sulfonate into 250 parts of water, stirring for 40min at the rotating speed of 550 r/min, adjusting the pH to 5 by using 0.03mol/L hydrochloric acid, adding 0.065 part of beta-glucosidase, stirring for 80min at the rotating speed of 450 r/min at 50 ℃, adding 0.15 part of horseradish peroxidase, adding 0.3 part of hydrogen peroxide with the mass concentration of 25%, stirring for 2h at the rotating speed of 450 r/min at 60 ℃, removing solvent water by distillation, adding the obtained product into 350 parts of ethanol, stirring for 20min at the rotating speed of 350 r/min at 30 ℃, centrifuging for 30min at the rotating speed of 9000 r/min, and drying the centrifuged precipitate for 6h at the temperature of 65 ℃ to obtain the modified sodium lignin sulfonate.
Example 4
Weighing the following raw materials in parts by weight: 65 parts of compound bacteria, 7 parts of a traditional Chinese medicine composition, 13 parts of subcritical hydrolysis products, 17 parts of modified black soil, 5 parts of humic acid, 2.5 parts of wetting agent, 4.5 parts of seaweed, 3.5 parts of modified sodium lignosulfonate and 11 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 40 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 2: 3.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 3 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 50mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.03.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 3.
The pretreatment of the black soil comprises the following steps: exposing black soil in the sun for 3 hours, then adding taro powder which is 0.3 time of the weight of the black soil block, locust corpses which are 0.2 time of the weight of the black soil block, berberine which is 0.015 time of the weight of the black soil block and clear water which is 2 times of the weight of the black soil block at the temperature of 60 ℃, stirring for 40 minutes, putting the mixture into a drying oven, and drying the mixture for 2 hours at the temperature of 50 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 50 parts of sodium lignosulfonate into 300 parts of water, stirring for 50min at the rotating speed of 600 revolutions/min, adjusting the pH to 6 by using 0.05mol/L hydrochloric acid, adding 0.08 part of beta-glucosidase, stirring for 100min at the rotating speed of 500 revolutions/min, adding 0.25 part of horseradish peroxidase, adding 0.5 part of hydrogen peroxide with the mass concentration of 30%, stirring for 3h at the rotating speed of 500 revolutions/min at 70 ℃, removing solvent water by distillation, adding the obtained product into 400 parts of ethanol, stirring for 25min at the rotating speed of 400 revolutions/min at 35 ℃, centrifuging for 40min at the rotating speed of 10000 revolutions/min, and drying the centrifuged precipitate for 8h at the temperature of 75 ℃ to obtain the modified sodium lignosulfonate.
Example 5
Weighing the following raw materials in parts by weight: 70 parts of compound bacteria, 8 parts of a traditional Chinese medicine composition, 14 parts of subcritical hydrolysis products, 18 parts of modified black soil, 6 parts of humic acid, 3 parts of wetting agent, 5 parts of seaweed, 4 parts of modified sodium lignosulfonate and 12 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 40 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 2: 3.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 3 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 50mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.03.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 3.
The pretreatment of the black soil comprises the following steps: exposing black soil in the sun for 3 hours, then adding taro powder which is 0.3 time of the weight of the black soil block, locust corpses which are 0.2 time of the weight of the black soil block, berberine which is 0.015 time of the weight of the black soil block and clear water which is 2 times of the weight of the black soil block at the temperature of 60 ℃, stirring for 40 minutes, putting the mixture into a drying oven, and drying the mixture for 2 hours at the temperature of 50 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 50 parts of sodium lignosulfonate into 300 parts of water, stirring for 50min at the rotating speed of 600 revolutions/min, adjusting the pH to 6 by using 0.05mol/L hydrochloric acid, adding 0.08 part of beta-glucosidase, stirring for 100min at the rotating speed of 500 revolutions/min, adding 0.25 part of horseradish peroxidase, adding 0.5 part of hydrogen peroxide with the mass concentration of 30%, stirring for 3h at the rotating speed of 500 revolutions/min at 70 ℃, removing solvent water by distillation, adding the obtained product into 400 parts of ethanol, stirring for 25min at the rotating speed of 400 revolutions/min at 35 ℃, centrifuging for 40min at the rotating speed of 10000 revolutions/min, and drying the centrifuged precipitate for 8h at the temperature of 75 ℃ to obtain the modified sodium lignosulfonate.
Example 6
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 35 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 40mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.015.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 2.5.
The pretreatment of the black soil comprises the following steps: exposing the black soil in the sun for 2.5 hours, then adding taro powder which is 0.2 time of the weight of the black soil block, locust corpses which are 0.15 time of the weight of the black soil block, berberine which is 0.01 time of the weight of the black soil block and clear water which is 1.5 times of the weight of the black soil block at the temperature of 55 ℃, stirring for 35 minutes, putting the mixture into an oven, and drying the mixture for 1.5 hours at the temperature of 45 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 40 parts of sodium lignin sulfonate into 250 parts of water, stirring for 40min at the rotating speed of 550 r/min, adjusting the pH to 5 by using 0.03mol/L hydrochloric acid, adding 0.065 part of beta-glucosidase, stirring for 80min at the rotating speed of 450 r/min at 50 ℃, adding 0.15 part of horseradish peroxidase, adding 0.3 part of hydrogen peroxide with the mass concentration of 25%, stirring for 2h at the rotating speed of 450 r/min at 60 ℃, removing solvent water by distillation, adding the obtained product into 350 parts of ethanol, stirring for 20min at the rotating speed of 350 r/min at 30 ℃, centrifuging for 30min at the rotating speed of 9000 r/min, and drying the centrifuged precipitate for 6h at the temperature of 65 ℃ to obtain the modified sodium lignin sulfonate.
Example 7
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g, and when the compound microbial agent is used, the microbial agent is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 35 kg/mu.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 40mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.025.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 2.5.
The pretreatment of the black soil comprises the following steps: exposing the black soil in the sun for 2.5 hours, then adding taro powder which is 0.2 time of the weight of the black soil block, locust corpses which are 0.15 time of the weight of the black soil block, berberine which is 0.01 time of the weight of the black soil block and clear water which is 1.5 times of the weight of the black soil block at the temperature of 55 ℃, stirring for 35 minutes, putting the mixture into an oven, and drying the mixture for 1.5 hours at the temperature of 45 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 40 parts of sodium lignin sulfonate into 250 parts of water, stirring for 40min at the rotating speed of 550 r/min, adjusting the pH to 5 by using 0.03mol/L hydrochloric acid, adding 0.065 part of beta-glucosidase, stirring for 80min at the rotating speed of 450 r/min at 50 ℃, adding 0.15 part of horseradish peroxidase, adding 0.3 part of hydrogen peroxide with the mass concentration of 25%, stirring for 2h at the rotating speed of 450 r/min at 60 ℃, removing solvent water by distillation, adding the obtained product into 350 parts of ethanol, stirring for 20min at the rotating speed of 350 r/min at 30 ℃, centrifuging for 30min at the rotating speed of 9000 r/min, and drying the centrifuged precipitate for 6h at the temperature of 65 ℃ to obtain the modified sodium lignin sulfonate.
Comparative example 1 (based on example 3, without modified Black soil)
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding humic acid, wetting agent, seaweed, modified sodium lignosulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified sodium lignosulfonate comprises the following steps: adding 40 parts of sodium lignin sulfonate into 250 parts of water, stirring for 40min at the rotating speed of 550 r/min, adjusting the pH to 5 by using 0.03mol/L hydrochloric acid, adding 0.065 part of beta-glucosidase, stirring for 80min at the rotating speed of 450 r/min at 50 ℃, adding 0.15 part of horseradish peroxidase, adding 0.3 part of hydrogen peroxide with the mass concentration of 25%, stirring for 2h at the rotating speed of 450 r/min at 60 ℃, removing solvent water by distillation, adding the obtained product into 350 parts of ethanol, stirring for 20min at the rotating speed of 350 r/min at 30 ℃, centrifuging for 30min at the rotating speed of 9000 r/min, and drying the centrifuged precipitate for 6h at the temperature of 65 ℃ to obtain the modified sodium lignin sulfonate.
Comparative example 2 (based on example 3, without modified sodium lignosulfonate)
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
The preparation method of the modified black soil comprises the following steps: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 40mm, and carrying out surface modification on the black soil by adopting a modifier under a weak acid environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.02.
the modifier is formed by mixing vinyl methyl dimethoxysilane and methacryloyloxyethyl maleic acid monoester according to the mass ratio of 1: 2.5.
The pretreatment of the black soil comprises the following steps: exposing the black soil in the sun for 2.5 hours, then adding taro powder which is 0.2 time of the weight of the black soil block, locust corpses which are 0.15 time of the weight of the black soil block, berberine which is 0.01 time of the weight of the black soil block and clear water which is 1.5 times of the weight of the black soil block at the temperature of 55 ℃, stirring for 35 minutes, putting the mixture into an oven, and drying the mixture for 1.5 hours at the temperature of 45 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
Comparative example 3 (based on example 3, without modified Black soil and modified sodium Lignosulfonate)
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding humic acid, wetting agent, seaweed and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
Comparative example 4 (on the basis of example 3, the modified blacksoil and the modified sodium lignin sulfonate were replaced with the ordinary blacksoil and the ordinary sodium lignin sulfonate)
Weighing the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of sodium lignosulphonate and 10 parts of cane sugar; uniformly mixing the subcritical hydrolysis product, the traditional Chinese medicine composition and the compound bacteria to obtain a mixture; adding the modified black soil, humic acid, wetting agent, seaweed, modified sodium lignin sulfonate and cane sugar into the mixture, and mixing again to obtain the compound microbial agent for effectively preventing and treating soil-borne diseases, wherein the number of effective viable bacteria in the compound microbial agent is more than or equal to 2 hundred million/g.
The compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
The mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1.5: 2.5.
the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 2 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
The preparation method of the subcritical hydrolysis product comprises the following steps: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
Comparative example 5
A commercially available microbial agent.
The crop variety is used as pepper, the microbial inoculum prepared in examples 1-7 and comparative examples 1-5 is used for root irrigation of pepper at the early stage of the incidence of pepper root rot, the using amount is 35 kg/mu, the root irrigation is carried out for 3 times at intervals of 1 time of 4d, after the root irrigation is carried out for the last 1 time and 5d, the prevention and treatment effect is calculated, the blank control group is not subjected to any treatment, and the result is shown in table 1.
TABLE 1
| Group of | Control effect (%) |
| Example 1 | 93.2 |
| Example 2 | 95.3 |
| Example 3 | 98.8 |
| Example 4 | 96.4 |
| Example 5 | 95.2 |
| Example 6 | 96.9 |
| Example 7 | 97.2 |
| Comparative example 1 | 78.1 |
| Comparative example 2 | 78.5 |
| Comparative example 3 | 64.6 |
| Comparative example 4 | 68.7 |
| Comparative example 5 | 85.3 |
| Blank control group | 0.7 |
From the results, the microbial agent prepared by the embodiment of the invention has good control effect, and particularly, the effect of controlling soil-borne diseases can be further improved by adding the modified black soil and the modified sodium lignosulfonate which are synergistic;
from the comparison of the data of example 3 with those of examples 6 and 7, it can be known that when the mass ratio of the black soil to the modifier is 1: when the content is 0.02, the modification effect on the black soil is optimal.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (10)
1. The compound microbial agent for effectively preventing and treating soil-borne diseases is characterized by comprising the following raw materials in parts by weight: 50-70 parts of compound bacteria, 4-8 parts of a traditional Chinese medicine composition, 10-14 parts of subcritical hydrolysis products, 12-18 parts of modified black soil, 2-6 parts of humic acid, 1-3 parts of wetting agent, 3-5 parts of seaweed, 2-4 parts of modified sodium lignosulfonate and 8-12 parts of cane sugar.
2. The compound microbial agent for effectively controlling soil-borne diseases according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 55-65 parts of compound bacteria, 5-7 parts of a traditional Chinese medicine composition, 11-13 parts of subcritical hydrolysis products, 13-17 parts of modified black soil, 3-5 parts of humic acid, 1.5-2.5 parts of a wetting agent, 3.5-4.5 parts of seaweed, 2.5-3.5 parts of modified sodium lignosulfonate and 9-11 parts of cane sugar.
3. The compound microbial agent for effectively controlling soil-borne diseases according to claim 2, which is characterized by comprising the following raw materials in parts by weight: 60 parts of compound bacteria, 6 parts of a traditional Chinese medicine composition, 12 parts of subcritical hydrolysis products, 15 parts of modified black soil, 4 parts of humic acid, 2 parts of wetting agent, 4 parts of seaweed, 3 parts of modified sodium lignosulfonate and 10 parts of cane sugar.
4. The compound microbial agent for effectively controlling soil-borne diseases according to claim 1, wherein the compound bacteria comprise purple lilac spore bacteria, trichoderma harzianum and bacillus subtilis.
5. The compound microbial agent for effectively controlling soil-borne diseases according to claim 4, wherein the mass ratio of the purple lilac spore bacteria to the trichoderma harzianum to the bacillus subtilis is 1: 1-2: 2-3.
6. The compound microbial inoculant for effectively controlling soil-borne diseases according to claim 1, wherein the preparation method of the traditional Chinese medicine composition comprises the following steps: respectively distilling herba Houttuyniae, folium Artemisiae Argyi and lalang grass rhizome to extract volatile oil, preparing volatile oil into volatile oil clathrate at 35 deg.C by ultrasonic clathration technique with beta-cyclodextrin, filtering the medicinal liquid after volatile oil extraction to obtain filtrate and residue, respectively soaking the residue in 60% ethanol, and heating and reflux-extracting for 1-3 times; then extracting with microwave to obtain extractive solution, mixing the volatile oil clathrate, filtrate and extractive solution, and spray drying to obtain Chinese medicinal composition.
7. The compound microbial inoculant for effectively controlling soil-borne diseases according to claim 1, wherein the subcritical hydrolysate is prepared by a method comprising: performing subcritical hydrolysis reaction on organic solid waste, then filtering to obtain filtrate, and performing spray drying to obtain a subcritical hydrolysis product, wherein the organic solid waste is at least one of plant ash, livestock and poultry manure, domestic garbage and domestic sludge.
8. The compound microbial agent for effectively controlling soil-borne diseases according to claim 1, wherein the preparation method of the modified black soil is as follows: the method comprises the following steps of crushing black soil blocks into particles with the particle size of 30-50mm, and carrying out surface modification on the black soil by adopting a modifier under a weakly acidic environment after pretreatment, wherein the modifier carries a carbon-carbon double bond capable of undergoing a polymerization reaction and a group capable of carrying out a bonding reaction with hydroxyl on the surface of the black soil, and the mass ratio of the black soil to the modifier is 1: 0.01-0.03.
9. The compound microbial inoculant for effectively controlling soil-borne diseases according to claim 8, wherein the pretreatment of the black soil is as follows: exposing black soil in the sun for 2-3 hours, then adding taro powder 0.1-0.3 time of the weight of black soil blocks, locust corpses 0.1-0.2 time of the weight of black soil blocks, berberine 0.005-0.015 time of the weight of black soil blocks and clear water 1-2 times of the weight of black soil blocks at the temperature of 50-60 ℃, stirring for 30-40 minutes, putting the mixture into a drying oven, and drying the mixture for 1-2 hours at the temperature of 40-50 ℃; cooling to room temperature, grinding, and sieving with 80 mesh sieve.
10. The use of the compound microbial inoculant according to any one of claims 1 to 9 for effectively controlling soil-borne diseases, wherein the use method comprises the following steps: the microbial inoculum is applied to crops in a broadcasting way or irrigated to roots, and the using amount is 30-40 kg/mu.
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