CN110859192A - Preparation for preventing and treating root rot of codonopsis pilosula and preparation method thereof - Google Patents
Preparation for preventing and treating root rot of codonopsis pilosula and preparation method thereof Download PDFInfo
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
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- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
Abstract
The invention discloses a preparation for preventing and treating root rot of pilose asiabell root and a preparation method thereof, belonging to the technical field of pesticides. The preparation for preventing and treating root rot of pilose asiabell root of the invention is composed of the following raw materials: aspergillus niger powder, alfalfa powder, chlorella extracting solution, pine nut shell, urea, Chinese lobelia extract, cimicifuga foetida, sinapyl alcohol, gallic acid, 4-hydroxy-benzoic acid and lupeol. According to the method, after the prepared preparation and seeds are mixed before the pilose asiabell root is cultivated, the root rot prevention effect of the pilose asiabell root is 97.3% -98.2%, the cold resistance is 92.9% -94.7%, the yield per mu is 310-317 kg/mu, the yield is increased by 29.2% -32.1% compared with that of conventional planting, no pesticide residue is generated, and the economic benefit is remarkable.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a preparation for preventing and treating root rot of codonopsis pilosula and a preparation method thereof.
Background
Wen radix Codonopsis is dried root of Angelica gigas nakai (Angelica pubescens Maxim. f. bisraratashan et Yuan) of Umbelliferae. Digging when seedlings just sprout in the early spring or when stems and leaves wither in the late autumn, removing fibrous roots and silt, drying to be semi-dry, piling for 2-3 days, softening and then drying to be completely dry. The surface is dark brown or dark brown, has longitudinal wrinkles, and has transverse long skin hole-like protrusions and slightly protruding fine root marks. Hard, soft when affected by moisture, grey-white cross section skin with a lot of scattered brown oil chambers, grey-yellow to yellow-brown wood, forming ring-brown color. Has special fragrance, and is bitter, pungent and slightly numb tongue. Contains osthole (C) calculated on dried product15H16O3) Not less than 0.50% of angelate containing dihydrocarveol (C)19H20O5) Not less than 0.080%.
The root rot of the Wen dangshen is mainly controlled by methods of intertillage weeding, drainage, selection of disease-free seedlings, seed soaking in 1:150 Bordeaux mixture, airing and sowing in the prior art, or 1000 times of 50 percent carbendazim wettable powder or 50 percent vertical withering water purifying agent 1500g/hm at the early stage of disease2Spraying 750kg of water for prevention and treatment, wherein the spraying is carried out for 1 time every 7 days and is carried out for 3-4 times continuously. However, the method has incomplete effect of preventing and treating root rot, a part of pesticide is remained in crops, and the method has certain limitation, and no effective preparation for preventing and treating root rot of codonopsis pilosula is available at present. Aiming at the defects, the prepared preparation can prevent and treat root rot and enhance the cold resistance of the radix codonopsis pilosulae adapting to the environment and the crop yield.
Disclosure of Invention
The invention aims to provide a preparation for preventing and treating root rot of pilose asiabell root and a preparation method thereof, wherein the preparation can be used for preventing and treating the root rot of pilose asiabell root and enhancing the cold resistance and crop yield of pilose asiabell root.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme.
A preparation for preventing and treating root rot of Wen radix codonopsis comprises the following raw materials in parts by weight: 41-47 parts of Aspergillus niger powder, 27-39 parts of alfalfa powder, 29.5-39.5 parts of chlorella extract, 23.5-27.5 parts of pine cone shell, 21-27 parts of urea, 17-25 parts of Chinese lobelia extract, 17-25 parts of cimicifuga foetida, 14-22 parts of sinapyl alcohol, 11-17 parts of gallic acid, 6-10 parts of 4-hydroxy-benzoic acid and 3-7 parts of lupeol.
Further, the preparation for preventing and treating root rot of codonopsis pilosula consists of the following raw materials in parts by weight: 44 parts of T-3 strain, 34.5 parts of chlorella extract, 25.5 parts of pine cone shell, 24 parts of urea, 21 parts of Chinese lobelia extract, 21 parts of cimicifuga foetida, 18 parts of sinapyl alcohol, 14 parts of gallic acid, 8 parts of 4-hydroxy-benzoic acid, 5 parts of lupeol and 95 parts of water.
The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula comprises the following steps:
1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder thereon, and performing solid state fermentation on the broad bean powder inoculated with the T-3 strain to obtain fermented broad bean powder X;
2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.2-1.5 cm, soaking in chlorella extractive solution and gallic acid for 35-40 min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; crushing the residual 3/4 cimicifugae rhizoma into 270-400 meshes of micro powder, adding 6-8 times of purified water, adjusting the pH to 6.4-7.0 by using gallic acid, heating to 45-60 ℃, and carrying out heat preservation and extraction for 40-50 min to obtain an extract R for later use;
3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 45-65 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight 6-11 times of that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 8-14 hours at 4-17 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.
Further, the temperature of the heat treatment in the step 1) is maintained at 68-120 ℃ for 15min, the temperature of the cold treatment is maintained at 35-42 ℃ for 25-30 min, and the temperature of the solid fermentation is maintained at 46-55 ℃ for 14-30 h.
Further, the protein-containing fermentation broth in the step 1) comprises 0.09-1.2 wt% of granulated sugar, 0.004-0.1 wt% of acetic acid, 0.07-1.3 wt% of cyclohexane carboxylate and 0.08-2.9 wt% of protein powder based on the total weight of the protein-containing fermentation broth.
Further, the air flow puffing temperature in the step 2) is controlled to be 82-86 ℃, and the vacuum degree is controlled to be 0.12-0.18 MPa.
Compared with the prior art, the invention has the beneficial technical effects that:
1) the invention can reduce the prevention effect on the germination rate and the root rot of the codonopsis pilosula seeds in the absence of any one of aspergillus niger bacterial powder and chlorella extract, the germination rate of the seeds is 96.3% under normal conditions, the prevention effect on the root rot is 97.7%, the germination rate and the root rot of the seeds are respectively reduced to 76.7% and 77.1% in the absence of the T-3 strain, the germination rate and the root rot are respectively reduced to 74.4% and 74.9% in the absence of the chlorella extract, and the germination rate and the root rot are respectively reduced to 59.2% and 53.8% in the absence of the chlorella extract.
2) According to the method, urea is not used, the acre yield of the codonopsis pilosula is obviously reduced, the reduction rate is 18%, the acre yield of the codonopsis pilosula is reduced by more or less when the content of the urea is increased or reduced, and the reduction rate is 10.1% -11.7%.
3) According to the method, after the prepared preparation and seeds are mixed before the pilose asiabell root is cultivated, the root rot prevention effect of the pilose asiabell root is 97.3% -98.2%, the cold resistance is 92.9% -94.7%, the yield per mu is 310-317 kg/mu, the yield is increased by 29.2% -32.1% compared with that of conventional planting, no pesticide residue is generated, and the economic benefit is remarkable.
Detailed Description
The technical solution of the present invention will be further specifically described below by way of specific examples. It is to be understood that the embodiments of the present invention are not limited to the following embodiments, and that any changes and/or modifications may be made to the present invention within the scope of the present invention.
Example 1
A preparation for preventing and treating root rot of Wen radix codonopsis comprises the following raw materials by weight: 41kg of Aspergillus niger powder, 27kg of alfalfa powder, 29.5kg of chlorella extract, 23.5kg of pine cone shell, 21kg of urea, 17kg of Chinese lobelia extract, 17kg of cimicifuga foetida, 14kg of sinapyl alcohol, 11kg of gallic acid, 6kg of 4-hydroxy-benzoic acid and 3kg of lupeol.
The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula comprises the following steps:
1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder on the Aspergillus niger powder, and performing solid state fermentation on the broad bean powder inoculated with the Aspergillus niger powder to obtain fermented broad bean powder X; the temperature of the heat treatment is maintained at 68 ℃ for 15min, the temperature of the cold treatment is maintained at 35 ℃ for 25min, and the temperature of the solid fermentation is maintained at 46 ℃ for 14 h; the protein-containing fermentation broth comprises 0.09 wt% of granulated sugar, 0.004 wt% of acetic acid, 0.07 wt% of cyclohexane carboxylate, and 0.08 wt% of protein powder, based on the total weight of the protein-containing fermentation broth;
2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.2cm, soaking in chlorella extractive solution and gallic acid for 35min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; pulverizing the rest 3/4 cimicifugae rhizoma into 270 mesh micropowder, adding 6 times of purified water, adjusting pH to 6.4 with gallic acid, heating to 45 deg.C, and leaching under heat preservation for 40min to obtain extract R; the air flow expansion temperature is controlled at 82 ℃, and the vacuum degree is controlled at 0.12 MPa;
3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 45 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into chloroform-methanol mixed solution with the weight 6 times that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 8 hours at 4 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.
Example 2
A preparation for preventing and treating root rot of Wen radix codonopsis comprises the following raw materials by weight: 44kg of Aspergillus niger powder, 33kg of alfalfa powder, 34.5kg of chlorella extract, 25.5kg of pine cone shell, 24kg of urea, 21kg of Chinese lobelia extract, 21kg of cimicifuga foetida, 18kg of sinapyl alcohol, 14kg of gallic acid, 8kg of 4-hydroxy-benzoic acid and 5kg of lupeol.
The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula comprises the following steps:
1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder on the Aspergillus niger powder, and performing solid state fermentation on the broad bean powder inoculated with the Aspergillus niger powder to obtain fermented broad bean powder X; the temperature of the heat treatment is maintained at 116 ℃ for 15min, the temperature of the cold treatment is maintained at 38 ℃ for 27min, and the temperature of the solid fermentation is maintained at 51 ℃ for 19 h; the protein-containing fermentation broth comprises 0.17 wt% of granulated sugar, 0.09 wt% of acetic acid, 1.1 wt% of cyclohexanecarboxylate, and 2.2 wt% of protein powder, based on the total weight of the protein-containing fermentation broth;
2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.3cm, soaking in chlorella extractive solution and gallic acid for 38min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; pulverizing the rest 3/4 cimicifugae rhizoma into 350 mesh micropowder, adding 7 times of purified water, adjusting pH to 6.8 with gallic acid, heating to 52 deg.C, and leaching under heat preservation for 45min to obtain extract R; the air flow expansion temperature is controlled at 84 ℃, and the vacuum degree is controlled at 0.15 MPa;
3) crushing pine cone shells, homogenizing with 4-hydroxy-benzoic acid at 55 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight 8 times that of the mixed homogeneous system, and fully stirring for 11 hours at 11 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.
Example 3
A preparation for preventing and treating root rot of Wen radix codonopsis comprises the following raw materials by weight: 47kg of Aspergillus niger powder, 39kg of alfalfa powder, 39.5kg of chlorella extract, 27.5kg of pine cone shell, 27kg of urea, 25kg of Chinese lobelia extract, 25kg of cimicifuga foetida, 22kg of sinapyl alcohol, 17kg of gallic acid, 10kg of 4-hydroxy-benzoic acid and 7kg of lupeol.
The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula comprises the following steps:
1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder on the Aspergillus niger powder, and performing solid state fermentation on the broad bean powder inoculated with the Aspergillus niger powder to obtain fermented broad bean powder X; the temperature of the heat treatment is maintained at 120 ℃ for 15min, the temperature of the cold treatment is maintained at 42 ℃ for 30min, and the temperature of the solid fermentation is maintained at 55 ℃ for 30 h; the protein-containing fermentation broth comprises 1.2 wt% of granulated sugar, 0.1 wt% of acetic acid, 1.3 wt% of cyclohexanecarboxylate, and 2.9 wt% of protein powder, based on the total weight of the protein-containing fermentation broth;
2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.5cm, soaking in chlorella extractive solution and gallic acid for 40min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; pulverizing the rest 3/4 cimicifugae rhizoma into 400 mesh micropowder, adding 8 times of purified water, adjusting pH to 7.0 with gallic acid, heating to 60 deg.C, and leaching under heat preservation for 50min to obtain extract R; the air flow expansion temperature is controlled at 86 ℃, and the vacuum degree is controlled at 0.18 MPa;
3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 65 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight being 11 times that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 14 hours at 17 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.
Examples of the experiments
The preparation for preventing and treating the root rot of the pilose asiabell root, which is prepared by the invention, is planted together with seeds before the pilose asiabell root is cultivated, the incidence rate and the prevention and treatment effect of the root rot are observed in the period of daily root rot attack of the pilose asiabell root, and the yield of the pilose asiabell root after harvesting is shown in table 1:
as can be seen from the above table, after the preparation prepared by the method is mixed with seeds before the cultivation of the Wen radix codonopsis pilosulae, the root rot prevention effect of the Wen radix codonopsis pilosulae is 97.3% -98.2%, the cold resistance is 92.9% -94.7%, the yield per mu is 310-317 kg/mu, the yield is increased by 29.2% -32.1% compared with the conventional planting, no pesticide residue is generated, and the economic benefit is remarkable.
Secondly, the following comparative tests are made aiming at the disease control effect of the aspergillus niger powder and the chlorella extract added in the formula on seed germination and root rot, wherein experiment 1 is to reduce the aspergillus niger powder, experiment 2 is to reduce the chlorella extract, and experiment 3 is to reduce both the aspergillus niger powder and the chlorella extract, as shown in table 2:
| item | Seed germination Rate (%) | Root rot control (%) |
| Experiment 1 | 76.7 | 77.1 |
| Experiment 2 | 74.4 | 74.9 |
| Experiment 3 | 59.2 | 53.8 |
As can be seen from Table 2, the lack of any one of the Aspergillus niger powder and the chlorella extract in the invention can reduce the germination rate and the root rot prevention effect of the Codonopsis pilosula seed, the germination rate of the seed is 96.3% under normal conditions, and the root rot prevention effect is 97.7%, while the seed germination rate and the root rot are respectively reduced to 76.7% and 77.1% under the lack of the Aspergillus niger powder, 74.4% and 74.9% under the lack of the chlorella extract, and 59.2% and 53.8% under the lack of the Chlorella extract.
Thirdly, the influence of the increase and decrease of urea in the formula on the yield of the pilose asiabell root is shown in table 3:
| item | Urea (kg) | Mu yield (kg/mu) | Yield reduction ratio (%) |
| Experiment 4 | 0 | 260 | 18.0 |
| Experiment 5 | 18 | 280 | 11.7 |
| Experiment 6 | 29 | 285 | 10.1 |
| Example 2 | 24 | 317 | - |
As can be seen from the above table, urea is not used in the method, the acre yield of the codonopsis pilosula is obviously reduced, the reduction rate is 18%, the acre yield of the codonopsis pilosula is reduced by more or less when the urea content is increased or reduced, and the reduction rate is 10.1% -11.7%.
The present invention may be embodied in other specific forms without departing from its spirit or essential characteristics. The above-described embodiments of the invention are to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (6)
1. A preparation for preventing and treating root rot of Wen radix codonopsis is characterized by comprising the following raw materials in parts by weight: 41-47 parts of Aspergillus niger powder, 27-39 parts of alfalfa powder, 29.5-39.5 parts of chlorella extract, 23.5-27.5 parts of pine cone shell, 21-27 parts of urea, 17-25 parts of Chinese lobelia extract, 17-25 parts of cimicifuga foetida, 14-22 parts of sinapyl alcohol, 11-17 parts of gallic acid, 6-10 parts of 4-hydroxy-benzoic acid and 3-7 parts of lupeol.
2. The preparation for preventing and treating root rot of pilose asiabell root according to claim 1, which is characterized by comprising the following raw materials in parts by weight: the traditional Chinese medicine composition comprises, by weight, 44 parts of aspergillus niger powder, 34.5 parts of chlorella extracting solution, 25.5 parts of pine cone shell, 24 parts of urea, 21 parts of Chinese lobelia extract, 21 parts of cimicifuga foetida, 18 parts of sinapyl alcohol, 14 parts of gallic acid, 8 parts of 4-hydroxy-benzoic acid and 5 parts of lupeol.
3. The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula as claimed in claim 1, characterized by comprising the following steps:
1) culturing Aspergillus niger powder on sterile protein-free fermentation broth without protein powder to stabilize the Aspergillus niger powder, inoculating cold-hot processed broad bean powder on the Aspergillus niger powder, and performing solid state fermentation on the broad bean powder inoculated with the Aspergillus niger powder to obtain fermented broad bean powder X;
2) cleaning 1/4 cimicifugae rhizoma and herba Lobeliae chinensis extract, removing impurities, cutting into blocks with thickness of 1.2-1.5 cm, soaking in chlorella extractive solution and gallic acid for 35-40 min, uniformly placing on a tray, and puffing in an airflow puffing tank to obtain puffed material W; crushing the residual 3/4 cimicifugae rhizoma into 270-400 meshes of micro powder, adding 6-8 times of purified water, adjusting the pH to 6.4-7.0 by using gallic acid, heating to 45-60 ℃, and carrying out heat preservation and extraction for 40-50 min to obtain an extract R for later use;
3) crushing pine cone shells, homogenizing the crushed pine cone shells and 4-hydroxy-benzoic acid at 45-65 ℃ to obtain a mixed homogeneous system, adding the mixed homogeneous system into a chloroform-methanol mixed solution with the weight 6-11 times of that of the mixed homogeneous system, and fully stirring the mixed homogeneous system for 8-14 hours at 4-17 ℃ to obtain pine cone shell slurry; filtering the stirred pine nut shell slurry by a filter membrane, and then rotatably evaporating the filtrate to dryness; extracting the evaporated material again by chloroform-methanol-water with volume ratio of 4: 3: 1.8, standing for layering, and collecting chloroform layer or evaporating chloroform layer to dryness to obtain mixture T; and then mixing the fermented broad bean powder X, the puffed material W, the extract R, the mixture T, urea, alfalfa powder, sinapyl alcohol, 4-hydroxy-benzoic acid and lupeol to obtain the preparation for preventing and treating the root rot of the Codonopsis pilosula.
4. The preparation method of the preparation for preventing and treating root rot of pilose asiabell root according to claim 3, characterized in that the temperature of heat treatment in step 1) is maintained at 68-120 ℃ for 15min, the temperature of cold treatment is maintained at 35-42 ℃ for 25-30 min, and the temperature of solid fermentation is maintained at 46-55 ℃ for 14-30 h.
5. The preparation method of the preparation for preventing and treating root rot of codonopsis pilosula in claim 3 or 4, wherein the protein-containing fermentation broth in the step 1) comprises 0.09-1.2 wt% of granulated sugar, 0.004-0.1 wt% of acetic acid, 0.07-1.3 wt% of cyclohexane carboxylate and 0.08-2.9 wt% of protein powder based on the total weight of the protein-containing fermentation broth.
6. The preparation method of the preparation for preventing and treating root rot of pilose asiabell root according to claim 3, characterized in that the air flow puffing temperature in the step 2) is controlled to 82-86 ℃, and the vacuum degree is controlled to 0.12-0.18 MPa.
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