CN106047766A - Compound microbiological antibacterial preparation for planting and method for preparing compound microbiological antibacterial preparation for planting - Google Patents
Compound microbiological antibacterial preparation for planting and method for preparing compound microbiological antibacterial preparation for planting Download PDFInfo
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Abstract
The invention belongs to the field of microbiological antibacterial agents, and particularly relates to a compound microbiological antibacterial preparation for planting and a method for preparing the compound microbiological antibacterial preparation for the planting. The problems that the quality of a crop decreases and an environment is polluted and which exist in the prior art can be solved. The compound microbiological antibacterial preparation for the planting is prepared from the following bacterial powder in parts by weight: 20 to 40 percent of bacillus subtilis ACCC10632, 20 to 40 percent of bacillus licheniformis ACCC10613, 20 to 40 percent of actinobacillus capsulatus ACCC11051 and 20 to 40 percent of bacillus laterosporus ACCC11079. The compound microbiological antibacterial preparation for the planting can be used for effectively suppressing multiple common soil-borne diseases, which are caused by a bacterium, a fungus and a nematode, of a crop, and moreover, has the advantages of being simple and convenient in using method, safe, free from residues, and antibacterial and bacteriostatic in a broad-spectrum manner.
Description
Technical field
The invention belongs to microbial antibacterial agent field, be specifically related to a kind of plantation complex microorganism antibiotic preparation and preparation
Method.
Background technology
China's agricultural production fertilising feature is that the most a large amount of chronic administration of chemical fertilizer make crops quality drop
Low, cause soil compaction, organic decline, soil and crop root Tiny ecosystem destructurized, result in soil especially and pass plant
Disease is spread unchecked.Common soilborne fungal pathogens have Semen Tritici aestivi and the banded sclerotial blight of Oryza sativa L., the head smut of Semen Maydis and soybean blight with
And the droop of melon bean and gray mold etc..Current disease prevention and control rely primarily on chemistry prevention and control, but chronic administration chemical agent
Also resulting in the problems such as medicament residual and environmental pollution, soil-borne disease is that the plant disease carrying out propagating for medium with soil is united
Claim.Soil-borne disease Biological control is studied always a focus and the problem of difficult point.
In soil, particularly plant rhizosphere perches many microbial resources with Biocontrol Potential, although their work
Affected very greatly by inherent and extrinsic factor with playing, but utilized the fireballing feature of microbial reproduction, executed after a large amount of artificial propagations
Enter in soil, root microecological environment, the breeding limiting soil-borne disease fungal pathogens and suppression soil-borne disease can be regulated and send out
Exhibition, demonstrates huge application potential.
Summary of the invention
The present invention is to solve crops quality reduction present in prior art, the problem polluting environment, it is provided that a kind of
Plantation complex microorganism antibiotic preparation and preparation method.
The present invention adopts the following technical scheme that realization:
A kind of plantation uses complex microorganism antibiotic preparation, including the mycopowder of following parts by weight: bacillus subtilis
ACCC10632,20%-40%, Bacillus licheniformis ACCC10613,20%-40%, actinobacillus capsulatus ACCC11051,20%-
40%, Brevibacillus laterosporus ACCC11079,20%-40%.
A kind of preparation method planting use complex microorganism antibiotic preparation, comprises the following steps that
The first step, actication of culture
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632, Bacillus licheniformis
(Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus capsulatus)
ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 bacterial strain are from the oil pipe kept
Transfer is connected in solid medium flat board, in 28 DEG C, after cultivating 48h.Take cultured solid medium flat board, choose with Inoculating needle
Single colony inoculation is in solid medium test tube, in 25-28 DEG C, cultivates 24-48h；
Second step, seed fermentation
With Inoculating needle choose appropriate bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 lawn connect
Planting and be put in 25-28 DEG C in equipped with the 500mL triangular flask of 150mL fluid medium, 130-180r/min constant-temperature table is cultivated, training
Support time 24-28h；
3rd step, liquid fermentation
Use hermetically sealed can ferments, and coefficient 0.7, by described bacillus subtilis (Bacillus subtilis subsp.
Subtilis) ACCC10632, Bacillus licheniformis (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus
(Actinobacillus capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus)
The seed culture fluid that ACCC11079 seed culture obtains is 0.5% ~ 3% to join in liquid fermentation medium according to inoculum concentration, sends out
Ferment temperature is 25 DEG C ~ 30 DEG C, and ventilation is 0.5 ~ 0.8m3/ h, rotating speed is 130 ~ 180r/min, and pH value is 6.5 ~ 7.5, during cultivation
Between 24 ~ 48h, obtain zymocyte liquid；
4th step, makes mycopowder
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632 that 3rd step is obtained,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the fermentation of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079
Bacterium solution, removes supernatant after being centrifuged and obtains wet thallus, and wet thallus is mixed homogeneously with the Pulvis Talci of equivalent, and 50 DEG C of vacuum dryings are to dry
Powdery, obtains dry bacteria；
5th step, mixes mycopowder
By bacillus subtilis obtained above (Bacillus subtilis subsp. Subtilis) ACCC10632, lichens
Bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the mycopowder of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079,
Mix homogeneously according to a certain percentage, obtains complex microorganism antibiotic preparation.
Solid medium described in the first step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar
15.0g, distilled water 1.0L, pH 7.0.
Seed culture medium described in second step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
Fermentation medium described in 3rd step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
The bacterium number of the mycopowder of each described strain is: >=5 × 109cfu/mL。
Described mycopowder ratio is: bacillus subtilis ACCC10632,20%-40%, Bacillus licheniformis ACCC10613,
20%-40%, actinobacillus capsulatus ACCC11051,20%-40%, Brevibacillus laterosporus ACCC11079,20%-40%.
Typical case's application case of the present invention:
1. complex microorganism antibiotic preparation is to wheat diseases preventing and treating and the application effect of volume increase
Test site: Yuncheng Yuanqu County；Participating in unit is Technology Bureau of Yuanqu County；Method is that complex microorganism is antibacterial
After preparation and foliage fertilizer compositional liquor mix according to the ratio of 1:100, spray is shone and (is executed with 30% chlopyrifos wettable powder on Caulis et Folium Tritici aestivi
Prescription formula is consistent).Result shows, after complex microorganism antibiotic preparation processes, wheat sharp eyespot sickness rate is 7%, and chemistry agriculture
The sickness rate of medicine is 16%.Complex microorganism antibiotic preparation is better than chemistry agriculture to wheat sharp eyespot and head blight disease-controlling effect
Medicine chlopyrifos, and increased production 10%.After complex micro organism fungicide processes, not only can increase yield, and significantly improve little
Wheat quality.
2. the complex microorganism antibiotic preparation application effect to Radix Betae disease control and volume increase
Test site: Yian County, Qiqihaer City of Heilongjiang Province；Participating in unit is Yian County work letter office；Method is Radix Betae nursery
Time, uniformly it being sprinkling upon field, nursery after complex microorganism Antibacterial Products is mixed soil, applied amount is that every square metre of nursery interpolation is compound
Microbial antibacterial formulation products 2 grams, then ploughs, whole, sowing.Complex microorganism antibiotic preparation has raising emergence rate, rush
Enter growth, the effect of shortening seedling raise period, compared with the comparison not adding this product, can carry and emerging the last week.It is transplanted to field
After between, in addition to promoting the effect of growth, moreover it is possible to more solid than Zao 10 days of the comparison without microbial inoculum product, and it is all to extend growth
10-15 days phase, 15-20% can be increased production.This product be watered 1:100 liquid spray three times (growth early, middle, late stage) have prevent and treat damping-off and
The remarkable efficacy of nematicide, can reduce the 4/5 of chemical pesticide consumption, average minimizing medication 1.5 times, can save medicine 1.5 yuan/mu.
The disease-controlling effect of complex microorganism antibiotic preparation not only period of duration whole to Radix Betae is notable, and can also increase production and raising product
Matter.
3. the complex microorganism antibiotic preparation application effect to orange tree disease control and volume increase
Test site: Zhongshan County, Hezhou City, Guangxi province；Participating in unit is Jin Hanwei bio tech ltd；Method is in test group
After being mixed according to the ratio of 1:100 with foliage fertilizer compositional liquor by complex microorganism antibiotic preparation, spray is shone to (right on orange tree blade face
Do not deal with according to group)；By complex microorganism antibiotic preparation and foliage fertilizer compositional liquor, according to the ratio pouring root of 1:50, (matched group is not made
Process).Result shows, after complex microorganism antibiotic preparation processes, test group orange tree bark cracking, broken leaf disease disease are than comparison
Group orange tree is less.Complex microorganism antibiotic preparation is notable to orange tree yellow twig disease-controlling effect, and the yield of Fructus Citri tangerinae
Improve 21%.
The invention has the beneficial effects as follows:
1. the present invention can suppress the common multiple soil-borne disease caused by antibacterial, fungus and nematicide of crops effectively；
2. the present invention and there is using method noresidue easy, safe, broad-spectrum antiseptic and antibacterial advantage.
Detailed description of the invention
Embodiment 1, a kind of preparation method planting use complex microorganism antibiotic preparation, comprise the following steps that
The first step, actication of culture
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632, Bacillus licheniformis
(Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus capsulatus)
ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 bacterial strain are from the oil pipe kept
Transfer is connected in solid medium flat board, in 28 DEG C, after cultivating 48h.Take cultured solid medium flat board, choose with Inoculating needle
Single colony inoculation is in solid medium test tube, in 25-28 DEG C, cultivates 24-48h；
Second step, seed fermentation
With Inoculating needle choose appropriate bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 lawn connect
Planting and be put in 25-28 DEG C in equipped with the 500mL triangular flask of 150mL fluid medium, 130-180r/min constant-temperature table is cultivated, training
Support time 24-28h；
3rd step, liquid fermentation
Use hermetically sealed can ferments, and coefficient 0.7, by described bacillus subtilis (Bacillus subtilis subsp.
Subtilis) ACCC10632, Bacillus licheniformis (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus
(Actinobacillus capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus)
The seed culture fluid that ACCC11079 seed culture obtains is 0.5% ~ 3% to join in liquid fermentation medium according to inoculum concentration, sends out
Ferment temperature is 25 DEG C ~ 30 DEG C, and ventilation is 0.5 ~ 0.8m3/ h, rotating speed is 130 ~ 180r/min, and pH value is 6.5 ~ 7.5, during cultivation
Between 24 ~ 48h, obtain zymocyte liquid；
4th step, makes mycopowder
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632 that 3rd step is obtained,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the fermentation of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079
Bacterium solution, removes supernatant after being centrifuged and obtains wet thallus, and wet thallus is mixed homogeneously with the Pulvis Talci of equivalent, and 50 DEG C of vacuum dryings are to dry
Powdery, obtains dry bacteria；
5th step, mixes mycopowder
By bacillus subtilis obtained above (Bacillus subtilis subsp. Subtilis) ACCC10632, lichens
Bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the mycopowder of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079,
Mix homogeneously according to a certain percentage, obtains complex microorganism antibiotic preparation.
Solid medium described in the first step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar
15.0g, distilled water 1.0L, pH 7.0.
Seed culture medium described in second step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
Fermentation medium described in 3rd step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
The bacterium number of the mycopowder of each described strain is: >=5 × 109cfu/mL。
Described mycopowder ratio is: bacillus subtilis ACCC10632,25%, Bacillus licheniformis ACCC10613,25%,
Actinobacillus capsulatus ACCC11051,25%, Brevibacillus laterosporus ACCC11079,25%.
Embodiment 2, a kind of preparation method planting use complex microorganism antibiotic preparation, comprise the following steps that
The first step, actication of culture
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632, Bacillus licheniformis
(Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus capsulatus)
ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 bacterial strain are from the oil pipe kept
Transfer is connected in solid medium flat board, in 28 DEG C, after cultivating 48h.Take cultured solid medium flat board, choose with Inoculating needle
Single colony inoculation is in solid medium test tube, in 25-28 DEG C, cultivates 24-48h；
Second step, seed fermentation
With Inoculating needle choose appropriate bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 lawn connect
Planting and be put in 25-28 DEG C in equipped with the 500ml triangular flask of 150ml fluid medium, 130-180r/min constant-temperature table is cultivated, training
Support time 24-28h；
3rd step, liquid fermentation
Use hermetically sealed can ferments, and coefficient 0.7, by described bacillus subtilis (Bacillus subtilis subsp.
Subtilis) ACCC10632, Bacillus licheniformis (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus
(Actinobacillus capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus)
The seed culture fluid that ACCC11079 seed culture obtains is 0.5% ~ 3% to join in liquid fermentation medium according to inoculum concentration, sends out
Ferment temperature is 25 DEG C ~ 30 DEG C, and ventilation is 0.5 ~ 0.8m3/ h, rotating speed is 130 ~ 180r/min, and pH value is 6.5 ~ 7.5, during cultivation
Between 24 ~ 48h, obtain zymocyte liquid；
4th step, makes mycopowder
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632 that 3rd step is obtained,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the fermentation of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079
Bacterium solution, removes supernatant after being centrifuged and obtains wet thallus, and wet thallus is mixed homogeneously with the Pulvis Talci of equivalent, and 50 DEG C of vacuum dryings are to dry
Powdery, obtains dry bacteria；
5th step, mixes mycopowder
By bacillus subtilis obtained above (Bacillus subtilis subsp. Subtilis) ACCC10632, lichens
Bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the mycopowder of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079,
Mix homogeneously according to a certain percentage, obtains complex microorganism antibiotic preparation.
Solid medium described in the first step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar
15.0g, distilled water 1.0L, pH 7.0.
Seed culture medium described in second step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
Fermentation medium described in 3rd step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
The bacterium number of the mycopowder of each described strain is: >=5 × 109cfu/mL。
Described mycopowder ratio is: bacillus subtilis ACCC10632,20%, Bacillus licheniformis ACCC10613,30%,
Actinobacillus capsulatus ACCC11051,30%, Brevibacillus laterosporus ACCC11079,20%.
Embodiment 3, a kind of preparation method planting use complex microorganism antibiotic preparation, comprise the following steps that
The first step, actication of culture
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632, Bacillus licheniformis
(Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus capsulatus)
ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 bacterial strain are from the oil pipe kept
Transfer is connected in solid medium flat board, in 28 DEG C, after cultivating 48h.Take cultured solid medium flat board, choose with Inoculating needle
Single colony inoculation is in solid medium test tube, in 25-28 DEG C, cultivates 24-48h；
Second step, seed fermentation
With Inoculating needle choose appropriate bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079 lawn connect
Planting and be put in 25-28 DEG C in equipped with the 500mL triangular flask of 150mL fluid medium, 130-180r/min constant-temperature table is cultivated, training
Support time 24-28h；
3rd step, liquid fermentation
Use hermetically sealed can ferments, and coefficient 0.7, by described bacillus subtilis (Bacillus subtilis subsp.
Subtilis) ACCC10632, Bacillus licheniformis (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus
(Actinobacillus capsulatus) ACCC11051, Brevibacillus laterosporus (Brevibacillus laterosporus)
The seed culture fluid that ACCC11079 seed culture obtains is 0.5% ~ 3% to join in liquid fermentation medium according to inoculum concentration, sends out
Ferment temperature is 25 DEG C ~ 30 DEG C, and ventilation is 0.5 ~ 0.8m3/ h, rotating speed is 130 ~ 180r/min, and pH value is 6.5 ~ 7.5, during cultivation
Between 24 ~ 48h, obtain zymocyte liquid；
4th step, makes mycopowder
Bacillus subtilis (Bacillus subtilis subsp. Subtilis) ACCC10632 that 3rd step is obtained,
Clothing bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the fermentation of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079
Bacterium solution, removes supernatant after being centrifuged and obtains wet thallus, and wet thallus is mixed homogeneously with the Pulvis Talci of equivalent, and 50 DEG C of vacuum dryings are to dry
Powdery, obtains dry bacteria；
5th step, mixes mycopowder
By bacillus subtilis obtained above (Bacillus subtilis subsp. Subtilis) ACCC10632, lichens
Bacillus cereus (Bacillus licheniformis) ACCC10613, actinobacillus capsulatus (Actinobacillus
Capsulatus) ACCC11051, the mycopowder of Brevibacillus laterosporus (Brevibacillus laterosporus) ACCC11079,
Mix homogeneously according to a certain percentage, obtains complex microorganism antibiotic preparation.
Solid medium described in the first step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar
15.0g, distilled water 1.0L, pH 7.0.
Seed culture medium described in second step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
Fermentation medium described in 3rd step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distillation
Water 1.0L, pH 7.0.
The bacterium number of the mycopowder of each described strain is: >=5 × 109cfu/mL。
Described mycopowder ratio is: bacillus subtilis ACCC10632,20%, Bacillus licheniformis ACCC10613,20%,
Actinobacillus capsulatus ACCC11051,20%, Brevibacillus laterosporus ACCC11079,40%.
Claims (7)
1. complex microorganism antibiotic preparation is used in a plantation, it is characterised in that: include the mycopowder of following parts by weight: hay spore
Bacillus ACCC10632,20%-40%, Bacillus licheniformis ACCC10613,20%-40%, actinobacillus capsulatus ACCC11051,
20%-40%, Brevibacillus laterosporus ACCC11079,20%-40%.
2. plant by the preparation method of complex microorganism antibiotic preparation for one kind, it is characterised in that: comprise the following steps that
The first step, actication of culture
Bacillus subtilis ACCC10632, Bacillus licheniformis ACCC10613, actinobacillus capsulatus ACCC11051, side spore bud
Spore bacillus ACCC11079 bacterial strain is connected to solid medium flat board from the oil pipe transfer kept, in 28 DEG C, after cultivating 48h, 3.
Take cultured solid medium flat board, choose single colony inoculation in solid medium test tube with Inoculating needle, in 25-28 DEG C, training
Support 24-48h；
Second step, seed fermentation
Appropriate bacillus subtilis ACCC10632, Bacillus licheniformis ACCC10613, actinobacillus capsulatus is chosen with Inoculating needle
ACCC11051, Brevibacillus laterosporus ACCC11079 lawn are inoculated in and put equipped with in the 500ml triangular flask of 150ml fluid medium
In 25-28 DEG C, 130-180r/min constant-temperature table is cultivated, incubation time 24-28h；
3rd step, liquid fermentation
Use hermetically sealed can ferments, and coefficient 0.7, by described bacillus subtilis ACCC10632, Bacillus licheniformis
The seed culture fluid that ACCC10613, actinobacillus capsulatus ACCC11051, Brevibacillus laterosporus ACCC11079 seed culture obtain
Being 0.5% ~ 3% to join in liquid fermentation medium according to inoculum concentration, fermentation temperature is 25 DEG C ~ 30 DEG C, ventilation is 0.5 ~
0.8m3/ h, rotating speed is 130 ~ 180r/min, and pH value is 6.5 ~ 7.5, and incubation time 24 ~ 48h obtains zymocyte liquid；
4th step, makes mycopowder
Bacillus subtilis ACCC10632 that 3rd step is obtained, Bacillus licheniformis ACCC10613, actinobacillus capsulatus
ACCC11051, the zymocyte liquid of Brevibacillus laterosporus ACCC11079, centrifugal after remove supernatant and obtain wet thallus, wet thallus with etc.
The Pulvis Talci mix homogeneously of amount, 50 DEG C of vacuum dryings, to powdered, obtain dry bacteria；
5th step, mixes mycopowder
By bacillus subtilis ACCC10632 obtained above, Bacillus licheniformis ACCC10613, actinobacillus capsulatus
ACCC11051, the mycopowder of Brevibacillus laterosporus ACCC11079, according to a certain percentage mix homogeneously, obtain complex microorganism antibacterial
Preparation.
A kind of preparation method planting use complex microorganism antibiotic preparation the most according to claim 2, it is characterised in that: the
Solid medium described in one step is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar 15.0g, distillation
Water 1.0L, pH 7.0.
A kind of preparation method planting use complex microorganism antibiotic preparation the most according to claim 2, it is characterised in that: the
Seed culture medium described in two steps is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distilled water 1.0L, pH
7.0。
A kind of preparation method planting use complex microorganism antibiotic preparation the most according to claim 2, it is characterised in that: the
Fermentation medium described in three steps is: peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, distilled water 1.0L, pH
7.0。
A kind of preparation method planting use complex microorganism antibiotic preparation the most according to claim 2, it is characterised in that: institute
The bacterium number of the mycopowder of each strain stated is: >=5 × 109cfu/mL。
A kind of preparation method planting use complex microorganism antibiotic preparation the most according to claim 2, it is characterised in that: institute
The mycopowder ratio stated is: bacillus subtilis ACCC10632,20%-40%, Bacillus licheniformis ACCC10613,20%-40%, pod
Film Actinobacillus ACCC11051,20%-40%, Brevibacillus laterosporus ACCC11079,20%-40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610602137.3A CN106047766A (en) | 2016-07-28 | 2016-07-28 | Compound microbiological antibacterial preparation for planting and method for preparing compound microbiological antibacterial preparation for planting |
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| CN106857141A (en) * | 2017-01-22 | 2017-06-20 | 邱德文 | A kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen |
| CN108101679A (en) * | 2018-01-10 | 2018-06-01 | 成都金昌旺农业科技有限公司 | A kind of preparation method for inhibiting soil nematodes fertilizer |
| CN108220185A (en) * | 2017-12-19 | 2018-06-29 | 华中农业大学 | A kind of application of biological control agent in citrus treelet yellow twig cause of disease is removed |
| CN111837766A (en) * | 2020-07-27 | 2020-10-30 | 华中农业大学 | Method for controlling pathogen of seedling phlobacterium phlogistii by composite microorganism treatment and application |
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| CN102002470A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof |
| CN102838398A (en) * | 2011-09-05 | 2012-12-26 | 麻林涛 | Special compound microbial fertilizer for peanuts and method for preparing special compound microbial fertilizer |
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| CN106857141A (en) * | 2017-01-22 | 2017-06-20 | 邱德文 | A kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen |
| CN108220185A (en) * | 2017-12-19 | 2018-06-29 | 华中农业大学 | A kind of application of biological control agent in citrus treelet yellow twig cause of disease is removed |
| CN108220185B (en) * | 2017-12-19 | 2021-08-17 | 华中农业大学 | Application of biological control agent in removing citrus sapling huanglongbing pathogen |
| CN108101679A (en) * | 2018-01-10 | 2018-06-01 | 成都金昌旺农业科技有限公司 | A kind of preparation method for inhibiting soil nematodes fertilizer |
| CN111837766A (en) * | 2020-07-27 | 2020-10-30 | 华中农业大学 | Method for controlling pathogen of seedling phlobacterium phlogistii by composite microorganism treatment and application |
| CN111837766B (en) * | 2020-07-27 | 2021-09-28 | 华中农业大学 | Method for controlling pathogen of seedling phlobacterium phlogistii by composite microorganism treatment and application |
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Application publication date: 20161026 |