CN102838398A - Special compound microbial fertilizer for peanuts and method for preparing special compound microbial fertilizer - Google Patents
Special compound microbial fertilizer for peanuts and method for preparing special compound microbial fertilizer Download PDFInfo
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Abstract
The invention relates to a special compound microbial fertilizer for peanuts and a method for preparing the special compound microbial fertilizer. The special compound microbial fertilizer for peanuts comprises compound microbial liquid and organic compound fertilizer, and comprises the following components by weight percent: 10-15% of ammonium sulfate, 15-25% of urea, 10-20% of single superphosphate, 5-15% of potassium chloride, 30-40% of grass carbon, 6-10% of the compound microbial liquid, 0.5-3% of borax and 0.5-3% of ammonium molybdate. The compound microbial liquid is prepared by mixing new bacillus lateraporus with a bacillus subtilis liquid. The special fertilizer can be used for solving the problems of excessive chemical fertilizer application amount, poor pertinence and unbalance nutrition of the conventional peanut fertilizer and instable application effect of the existing microbial fertilizer and the like, and both the production and income can be obviously increased after the application.
Description
Technical field
The present invention relates to kind of special fertilizer for peanut material and preparation method thereof, particularly the specific complex microbial fertilizer and preparation method thereof of cultivating peanut.
Background technology
Peanut is the important cash crop of China, and it is rich in fat and protein, i.e. edible again can be feeding; Promptly be important industrial raw material, be large exporting again, of many uses, higher status is arranged in national economy.Current, China's peanut mean yield in the 3000kg/ sq-km left and right sides farce wander, its yield potential is bigger, and it is carried out scientific and reasonable fertilising, will be the important channel of improving output.Therefore, need fertile characteristics according to peanut, it is comprehensive to develop a kind of nutrient, and slow-releasing is better, can fully satisfy peanut to nutrient demand, brings into play fertilizer effect to greatest extent, and the dedicated fertilizer that improves peanut yield and quality is particularly urgent.
Microbial fertilizer more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency; This provides sizable development space for its exploitation, popularization, application; Particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.But the problem of microbial fertilizer existence at present is that effective bacterium number is not up to standard, the quality guaranteed period is too short, thereby effect is unstable.
Summary of the invention
The objective of the invention is to; Overcome in the production owing to unreasonable fertilising, execute partially that reason such as chemical nitrogen fertilizer causes that output reduces, degradation is not enough under the quality; Provide a kind of and match, be added with the special-purpose fertile of microbiobacterial agent with peanut nutrient demand characteristic; Both improve peanut yield, improved peanut quality, reduced the interpolation of chemical fertilizer again.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and all equipment and starting material etc. all can be buied from market or the industry is commonly used.
The present invention adopts following technical scheme to realize:
Peanut specific complex microbial fertilizer of the present invention comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, by weight percentage: ammonium sulfate 10-15%; Urea 15-25%, general calcium 10-20%, Repone K 5-15%; Peat composed of rotten mosses 30-40%, composite microbial bacteria liquid 6-10%, borax 0.5-3%; Ammonium molybdate 0.5-3% adds up to 100%.
Described composite microbial bacteria liquid makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus) and subtilis (Bacillus subtilis), mixes according to arbitrary proportion again.
Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-6:1-6, is more preferably 1:1.
The present invention adopts new bacillus laterosporus (Bacillus laterosporus), specifically is named as " new No. 1 bacterial strain of lateral bud spore bacillus ", and preserving number is CGMCC No.5090.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
New No. 1 bacterial strain of lateral bud spore bacillus is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Preservation biomaterial name is called: new lateral bud spore bacillus No. 1; Deposit number is CGMCC No.5090; Biology name bacillus laterosporus Bacillus laterosporus, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The biological characteristics of this bacterial classification is that thalline is shaft-like, belongs to bacillus, and its cell size is 0.15～0.18 * 215～510um; Gram-positive; Can be changed into feminine gender, gemma is oval, adnation, middle life or nearly middle giving birth to; Sporangiocyst expands, and free gemma is on one side than the other side thick (canoe shape).Can utilize multiple nitrogenous source, carbon source, growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.2.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 20 minutes and drying to the poor environment strong stress resistance.
The present invention adopts new subtilis (Bacillus subtilis), specifically is named as " new No. 1 bacterial strain of subtilis ", and preserving number is CGMCC No.5091.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
New No. 1 bacterial strain of subtilis is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Preservation biomaterial name is called: new subtilis No. 1; Deposit number is CGMCC No.5091; Biology name subtilis Bacillus subtilis, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The biological characteristics of this bacterial classification is that thalline is shaft-like, the gemma aerophil of making a living, and its gemma can resist high heat (still can survive in following 30 minutes at 100 ℃), and can in the withered grass leach liquor, grow, and its gemma is ellipse or long barrel shape, 0.6～0.9 * 1.0～1.5 μ m.Form in sporangium central authorities or nearly central part, generally between waist, germinate.Shaft-like 0.3～0.7 * 2～3 μ m of vegetative cell, isolated or one-tenth short chain, rod end is semicircle.Bacterium colony is spreading property, and scabriculous or folding are greyish white, and be yellowish even yellow.When growing in the liquid medium within, the normal wrinkle mould that forms.Aerophil.Available protein, multiple sugar and starch decompose tryptophane and form indoles.Growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.1.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 15 minutes and drying to the poor environment strong stress resistance.
Further, the prescription of peanut specific complex microorganism fertilizer of the present invention does, by weight percentage: by weight percentage: ammonium sulfate 11%, urea 20%, general calcium 13%, Repone K 10%, the peat composed of rotten mosses 36%, composite microbial bacteria liquid 8%, borax 1%, ammonium molybdate 1%.
In the present invention, said composite microbial bacteria liquid is prepared from following new cultural method:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation; Mix with turfy soil in the ratio of 1:2-10 then, granulation, oven drying at low temperature obtains bacillus laterosporus microbial inoculum and bacillus subtilis microbial agent respectively;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
The side spore bacillus brevis that fermentation is obtained mixes with any weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-6:1-6, is more preferably 1:1.
Again with all the other siccatives pulverize respectively, proportional mixing, add the above-mentioned complex micro organism fungicide of processing through fermentation absorption, mixing and through oven drying at low temperature, granulation, pack makes peanut specific complex microbial fertilizer of the present invention.
Fertilizer specially for peanut material of the present invention is compared the positively effect that is had with existing fertilizer and is:
Peanut specific complex microorganism fertilizer of the present invention has added new microbial strains; And adopt new cultivation and fermentation method to prepare new microbiobacterial agent; Then to the growth characteristics and the nutritional needs characteristic of peanut, itself and composite fertilizer are mixed with according to a certain percentage form again, test shows; Fertilizer specially for peanut material of the present invention can reduce cost, increase output, improve quality, thereby improves the market competitiveness of similar peanut products.The present invention through the statistical study of field test results, determines optimum formula and formula rate according to the nutrition absorption characteristic and the available nutrient of soil situation of peanut.
Below in conjunction with embodiment the present invention is specifically described, the present invention also can realize through other embodiments that does not break away from its spirit or essential feature.Therefore, below embodiment, just illustrate, be not only, all are within the scope of the present invention or be equal to change in the scope of the present invention and all comprised by the present invention.
Embodiment:
Embodiment 1:
The one specific complex microorganism fertilizer of cultivating peanut, its prescription do, by weight percentage: ammonium sulfate 11%, urea 20%, general calcium 13%, Repone K 10%, the peat composed of rotten mosses 36%, composite microbial bacteria liquid 8%, borax 1%, ammonium molybdate 1%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:1 again.
The preparation technology of bacillus laterosporus bacterium liquid is following:
Earlier the bacillus laterosporus bacterial classification is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The preparation technology of subtilis bacterium liquid is following:
Earlier Bacillus subtilis strain is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the 1:1 weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes peanut specific complex microbial fertilizer of the present invention.
Embodiment 2
The one specific complex microorganism fertilizer of cultivating peanut, its prescription do, by weight percentage: ammonium sulfate 10%, urea 25%, general calcium 10%, Repone K 15%, the peat composed of rotten mosses 30%, composite microbial bacteria liquid 6%, borax 2%, ammonium molybdate 2%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:2 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 1:2 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes peanut specific complex microbial fertilizer of the present invention.
Embodiment 3
The one specific complex microorganism fertilizer of cultivating peanut, its prescription do, by weight percentage: ammonium sulfate 15%, urea 15%, general calcium 20%, Repone K 5%, the peat composed of rotten mosses 31%, composite microbial bacteria liquid 10%, borax 3%, ammonium molybdate 1%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 2:1 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 2:1 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes peanut specific complex microbial fertilizer of the present invention.
The manure trial of the present invention's " peanut specific complex microbial fertilizer " on peanut:
1. test materials and method
1.1 material
This is tested in whole ground on June 2nd, 2008, fertilizer bottom application, sowing on June 3.Growing period waters 2 times, prevention and elimination of disease and pests 1 time.The single meter of receiving in sub-district produces picked at random 1m during results
2Plant does indoor species test, and control measures are with local high yield land for growing field crops standard.
2 test-results and analysis
2.1 execute "
Peanut specific complex microbial fertilizer" peanut become the influence of product factor.
Execute "
Peanut specific complex microbial fertilizer" improved peanut and become the product factor.
Can find out by table 1: use "
Peanut specific complex microbial fertilizer" peanut strain grain number and thousand seed weight: all handle high than other.
Table 1 peanut yield survey on Factors table
| Handle | Mu strain number (strain/mu) | Strain grain number | Thousand seed weight |
| 1 | 9600 | 103.2 | 201.3 |
| 2 2 | 9600 | 109.8 | 218.0 |
| 3 | 9600 | 106.8 | 206.0 |
2. 2 use "
Peanut specific complex microbial fertilizer" to the influence of peanut yield
Sub-district whole district yield result table:
Can find out from last table, peanut use "
Peanut specific complex microbial fertilizer" afterwards effect is remarkable, F=24. 89
>LSR0.05=1.20kg reaches utmost point level of signification, and further analysis revealed is handled 2 effect of increasing production 2.l7kg, surpasses the LSR0.05=1.20 level, reaches level of signification, and raising the output is amounted to 667m more than 24.54%
2Raising the output 48.2kg.
3 brief summaries
Can increase strain grain number and thousand seed weight 3.1 peanut is used the specific complex microorganism fertilizer, thereby significantly increase output.
Claims (5)
1. the specific complex microbial fertilizer of cultivating peanut comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, it is characterized in that consisting of by weight percentage: ammonium sulfate 10-15%; Urea 15-25%, general calcium 10-20%, Repone K 5-15%; Peat composed of rotten mosses 30-40%, composite microbial bacteria liquid 6-10%, borax 0.5-3%; Ammonium molybdate 0.5-3% adds up to 100%; Described composite microbial bacteria liquid is that CGMCC No.5090 and subtilis (Bacillus subtilis), preserving number are that CGMCC No.5091 makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus), preserving number, mixes according to arbitrary proportion again.
2. peanut specific complex microbial fertilizer as claimed in claim 1, by weight percentage: ammonium sulfate 11%, urea 20%, general calcium 13%, Repone K 10%, the peat composed of rotten mosses 36%, composite microbial bacteria liquid 8%, borax 1%, ammonium molybdate 1%.
3. according to claim 1 or claim 2 peanut specific complex microbial fertilizer, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1-6:1-6.
4. peanut specific complex microbial fertilizer as claimed in claim 3, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1:1.
5. like the preparation method of the described peanut specific complex of claim 1-4 microbial fertilizer, it is characterized in that comprising the steps:
The first step: preparation microbial inoculum:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8;
Second step: will ferment the side spore bacillus brevis liquid that obtains and subtilis liquid mix with said weight ratio and obtain composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use;
The 3rd step: again with all the other siccatives pulverize respectively, proportional mixing, add the complex micro organism fungicide that second step processed through fermentation absorption, mixing also promptly gets through oven drying at low temperature, granulation, pack.
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| CN103396261A (en) * | 2013-07-02 | 2013-11-20 | 河南天宇肥业有限公司 | Formula fertilizer special for peanut |
| CN103539579A (en) * | 2013-10-12 | 2014-01-29 | 合肥科友生物科技有限公司 | Peanut special-purpose organic-inorganic composite biological medicinal fertilizer and preparation method thereof |
| CN103756930A (en) * | 2013-12-03 | 2014-04-30 | 青岛润地丰科技有限公司 | Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof |
| CN104446970A (en) * | 2014-11-27 | 2015-03-25 | 河北省农林科学院农业资源环境研究所 | Organic and inorganic microbial fertilizer special for peanuts and preparation method thereof |
| CN106047766A (en) * | 2016-07-28 | 2016-10-26 | 唐清池 | Compound microbiological antibacterial preparation for planting and method for preparing compound microbiological antibacterial preparation for planting |
| CN106278555A (en) * | 2016-07-28 | 2017-01-04 | 四川茂耘农业科技有限公司 | One cultivates peanut special microorganism slow-release fertilizer and preparation method thereof |
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| CN108102972A (en) * | 2018-01-31 | 2018-06-01 | 山东省花生研究所（山东省农业科学院花生工程技术研究中心） | A kind of preparation method and application of the compound biocontrol fungicide of cure plant disease of peanut |
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| CN103756930B (en) * | 2013-12-03 | 2015-06-10 | 山东省花生研究所 | Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof |
| CN104446970A (en) * | 2014-11-27 | 2015-03-25 | 河北省农林科学院农业资源环境研究所 | Organic and inorganic microbial fertilizer special for peanuts and preparation method thereof |
| CN106047766A (en) * | 2016-07-28 | 2016-10-26 | 唐清池 | Compound microbiological antibacterial preparation for planting and method for preparing compound microbiological antibacterial preparation for planting |
| CN106278555A (en) * | 2016-07-28 | 2017-01-04 | 四川茂耘农业科技有限公司 | One cultivates peanut special microorganism slow-release fertilizer and preparation method thereof |
| CN107141141A (en) * | 2017-07-07 | 2017-09-08 | 辽宁玉宝农业科技有限公司 | One kind of special fertilizer for peanut and preparation method thereof |
| CN108102972A (en) * | 2018-01-31 | 2018-06-01 | 山东省花生研究所（山东省农业科学院花生工程技术研究中心） | A kind of preparation method and application of the compound biocontrol fungicide of cure plant disease of peanut |
| CN109265265A (en) * | 2018-11-23 | 2019-01-25 | 王变芝 | Peanut cultivation special bio bacterial manure and preparation method thereof |
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