CN102424615A - Special composite microbial fertilizer for tobacco use and its preparation method - Google Patents
Special composite microbial fertilizer for tobacco use and its preparation method Download PDFInfo
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Abstract
The invention relates to a special composite microbial fertilizer for tobacco use and its preparation method. The fertilizer comprises a composite microbial solution and an organic-inorganic compound fertilizer that total 100% by weight percent. The composite microbial solution is mixed by a bacterial solution of new bacillus laterosporus and a bacterial solution of bacillus subtilis. The special fertilizer of the invention can provide a solution to the problems of excessive application of chemical fertilizers, poor pertinence, imbalanced nutrition, unstable application effect of existing microbial fertilizers for tobacco, and can achieve obvious increase of production and income after application.
Description
Technical field
The present invention relates to a kind of fertilizer special for tobacco material and preparation method thereof, particularly the specific complex microbial fertilizer and preparation method thereof that grows tobacco.
Background technology
Tobacco is that leaf is used cash crop, has happiness potassium, phosphorus and the characteristics of few nitrogenous fertilizer are produced at present and gone up a large amount of inorganic chemical fertilizers that adopt; Cause the physico-chemical property of tobacco-growing soil to worsen; Soil compaction, ventilation property is poor, and preserve moisture and fertility power reduces; In addition, excessively using chemical fertilizer also can cause environment and have a strong impact on.When using excessive chemical fertilizer, the nutrient that is not absorbed by earth will lose with current, and near the water source overnutrition causing quickens algae grows, influences the whole ecological system.And cigarette industry requires tobacco leaf to have good surface appearance quality and inner quality, and chemical ingredients nicotine, reducing sugar, total reducing sugar, protein, potassium and cl content in the tobacco leaf are suitable, and total nitrogen, total reducing sugar are answered eurythmy with the nicotine ratio.
Microbial fertilizer more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency; This provides sizable development space for its exploitation, popularization, application; Particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.But the problem of microbial fertilizer existence at present is that effective bacterium number is not up to standard, the quality guaranteed period is too short, thereby effect is unstable.
Summary of the invention
The objective of the invention is to; Overcome in the production owing to unreasonable fertilising, execute partially that reason such as chemical fertilizer causes that output reduces, degradation is not enough under the quality; Provide a kind of and match, be added with the special-purpose fertile of microbiobacterial agent with tobacco nutrient demand characteristic; Both improve tobacco production, improved cigarette quality, reduced the interpolation of chemical fertilizer again.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and all equipment and starting material etc. all can be buied from market or the industry is commonly used.
The present invention adopts following technical scheme to realize:
Tobacco specific complex microbial fertilizer of the present invention comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, by weight percentage: ammonium sulfate 5-10%, diammonium phosphate 12-20%; Fused(calcium magnesium)phosphate 2-10%, vitriolate of tartar 20-30%, peat composed of rotten mosses 30-40%; Composite microbial bacteria liquid 5-15%, zinc sulfate 0.1-2%, ferrous sulfate 0.1-2%; Ammonium molybdate 0.1-3%, manganous sulfate 0.1-3% adds up to 100%.
Described composite microbial bacteria liquid makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus) and subtilis (Bacillus subtilis), mixes according to arbitrary proportion again.
Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-6:1-6, is more preferably 1:1.
The present invention adopts new bacillus laterosporus (Bacillus laterosporus), specifically is named as " new No. 1 bacterial strain of lateral bud spore bacillus ", and preserving number is CGMCC No.5090.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
New No. 1 bacterial strain of lateral bud spore bacillus is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Deposit number is CGMCC No.5090; Classification called after bacillus laterosporus (Bacillus laterosporus); Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The biological characteristics of this bacterial classification is that thalline is shaft-like, belongs to bacillus, and its cell size is 0.15～0.18 * 215～510um; Gram-positive; Can be changed into feminine gender, gemma is oval, adnation, middle life or nearly middle giving birth to; Sporangiocyst expands, and free gemma is on one side than the other side thick (canoe shape).Can utilize multiple nitrogenous source, carbon source, growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.2.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 20 minutes and drying to the poor environment strong stress resistance.
The present invention adopts new subtilis (Bacillus subtilis), specifically is named as " new No. 1 bacterial strain of subtilis ", and preserving number is CGMCC No.5091.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
New No. 1 bacterial strain of subtilis is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Deposit number is CGMCC No.5091; Classification called after subtilis (Bacillus subtilis); Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The biological characteristics of this bacterial classification is that thalline is shaft-like, the gemma aerophil of making a living, and its gemma can resist high heat (still can survive in following 30 minutes at 100 ℃), and can in the withered grass leach liquor, grow, and its gemma is ellipse or long barrel shape, 0.6～0.9 * 1.0～1.5 μ m.Form in sporangium central authorities or nearly central part, generally between waist, germinate.Shaft-like 0.3～0.7 * 2～3 μ m of vegetative cell, isolated or one-tenth short chain, rod end is semicircle.Bacterium colony is spreading property, and scabriculous or folding are greyish white, and be yellowish even yellow.When growing in the liquid medium within, the normal wrinkle mould that forms.Aerophil.Available protein, multiple sugar and starch decompose tryptophane and form indoles.Growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.1.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 15 minutes and drying to the poor environment strong stress resistance.
Further, the prescription of tobacco specific complex microorganism fertilizer of the present invention does, by weight percentage: by weight percentage: ammonium sulfate 8%; Diammonium phosphate 17%, fused(calcium magnesium)phosphate 6%, vitriolate of tartar 23%; The peat composed of rotten mosses 34%, composite microbial bacteria liquid 10%, zinc sulfate 0.5%; Ferrous sulfate 0.5%, ammonium molybdate 0.5%, manganous sulfate 0.5%.
In the present invention, said composite microbial bacteria liquid is prepared from following new cultural method:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation; Mix with turfy soil in the ratio of 1:2-10 then, granulation, oven drying at low temperature obtains bacillus laterosporus microbial inoculum and bacillus subtilis microbial agent respectively;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
The side spore bacillus brevis that fermentation is obtained mixes with any weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-6:1-6, is more preferably 1:1.
Again with all the other siccatives pulverize respectively, proportional mixing, add the above-mentioned complex micro organism fungicide of processing through fermentation absorption, mixing and through oven drying at low temperature, granulation, pack makes tobacco specific complex microbial fertilizer of the present invention.
Fertilizer special for tobacco material of the present invention is compared the positively effect that is had with existing fertilizer and is:
Tobacco specific complex microorganism fertilizer of the present invention has added new microbial strains, and adopts new cultivation and fermentation method to prepare new microbiobacterial agent, and test shows, adds fertilizer special for tobacco of the present invention and can improve the rate of growth of tobacco and improve the quality of tobacco.The quality of tobacco all has clear improvement like moisture content, density, fibrousness etc.Simultaneously, the present invention is directed to growth characteristics and the nutritional needs characteristic of tobacco,, determine optimum formula and formula rate, more effectively reduce edatope and pollute, prevention disease rate of growth and strengthen drought resisting, cold-resistant ability through the statistical study of field test results.In a word, tobacco specific complex microbial fertilizer of the present invention can reduce cost, increase output, improve quality, thereby improves the market competitiveness of similar tobacco product.
Below in conjunction with embodiment the present invention is specifically described, the present invention also can realize through other embodiments that does not break away from its spirit or essential feature.Therefore, below embodiment, just illustrate, be not only, all are within the scope of the present invention or be equal to change in the scope of the present invention and all comprised by the present invention.
Embodiment:
Embodiment 1:
The one specific complex microorganism fertilizer that grows tobacco, its prescription do, by weight percentage: ammonium sulfate 8%, diammonium phosphate 17%; Fused(calcium magnesium)phosphate 6%, vitriolate of tartar 23%, the peat composed of rotten mosses 34%, composite microbial bacteria liquid 10%; Zinc sulfate 0.5%, ferrous sulfate 0.5%, ammonium molybdate 0.5%, manganous sulfate 0.5%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:1 again.
The preparation technology of bacillus laterosporus bacterium liquid is following:
Earlier the bacillus laterosporus bacterial classification is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The preparation technology of subtilis bacterium liquid is following:
Earlier Bacillus subtilis strain is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the 1:1 weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes tobacco specific complex microbial fertilizer of the present invention.
Embodiment 2
The one specific complex microorganism fertilizer that grows tobacco, its prescription do, by weight percentage: ammonium sulfate 5%, diammonium phosphate 20%; Fused(calcium magnesium)phosphate 2%, vitriolate of tartar 20%, the peat composed of rotten mosses 40%, composite microbial bacteria liquid 8%; Zinc sulfate 2%, ferrous sulfate 1%, ammonium molybdate 1%, manganous sulfate 1%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:2 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 1:2 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes tobacco specific complex microbial fertilizer of the present invention.
Embodiment 3
The one specific complex microorganism fertilizer that grows tobacco, its prescription do, by weight percentage: ammonium sulfate 10%, diammonium phosphate 12%; Fused(calcium magnesium)phosphate 10%, vitriolate of tartar 30%, the peat composed of rotten mosses 30%, composite microbial bacteria liquid 5%; Zinc sulfate 0.5%, ferrous sulfate 0.5%, ammonium molybdate 1%, manganous sulfate 1%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 2:1 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 2:1 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes tobacco specific complex microbial fertilizer of the present invention.
This microbial fertilizer is applied on the tobacco, obtains positive effect:
Use 100 kilograms of tobacco specific complex microorganism fertilizers of the present invention for every mu; Through repeatedly measuring, observing; Tobacco is contrast growth by 35.8% of mean density after 1 year, and plant height also increases by 28.6%, and tobacco growing speed speeds 13.5%; The color of grass is kept fresh and tender for a long time, and it is more lasting that soil moisture keeps.
Claims (5)
1. the specific complex microbial fertilizer that grows tobacco comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, it is characterized in that consisting of by weight percentage: ammonium sulfate 5-10%, diammonium phosphate 12-20%; Fused(calcium magnesium)phosphate 2-10%, vitriolate of tartar 20-30%, peat composed of rotten mosses 30-40%; Composite microbial bacteria liquid 5-15%, zinc sulfate 0.1-2%, ferrous sulfate 0.1-2%; Ammonium molybdate 0.1-3%, manganous sulfate 0.1-3% adds up to 100%; Described composite microbial bacteria liquid is that CGMCC No.5090 and subtilis (Bacillus subtilis), preserving number are that CGMCC No.5091 makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus), preserving number, mixes according to arbitrary proportion again.
2. tobacco specific complex microbial fertilizer as claimed in claim 1, by weight percentage: ammonium sulfate 8%, diammonium phosphate 17%; Fused(calcium magnesium)phosphate 6%, vitriolate of tartar 23%, the peat composed of rotten mosses 34%; Composite microbial bacteria liquid 10%, zinc sulfate 0.5%, ferrous sulfate 0.5%; Ammonium molybdate 0.5%, manganous sulfate 0.5%.
3. according to claim 1 or claim 2 tobacco specific complex microbial fertilizer, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1-6:1-6.
4. tobacco specific complex microbial fertilizer as claimed in claim 3, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1:1.
5. like the preparation method of the described tobacco specific complex of claim 1-4 microbial fertilizer, it is characterized in that comprising the steps:
The first step: preparation microbial inoculum:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8;
Second step: will ferment the side spore bacillus brevis liquid that obtains and subtilis liquid mix with said weight ratio and obtain composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use;
The 3rd step: again with all the other siccatives pulverize respectively, proportional mixing, add the complex micro organism fungicide that second step processed through fermentation absorption, mixing also promptly gets through oven drying at low temperature, granulation, pack.
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| CN106316653A (en) * | 2016-08-31 | 2017-01-11 | 潘爱华 | Compound microbial fertilizer produced from tobacco waste |
| CN112136814A (en) * | 2020-09-28 | 2020-12-29 | 山东胜伟盐碱地科技有限公司 | Plant growth agent for promoting tobacco growth and preparation and application thereof |
| CN113717507A (en) * | 2021-10-07 | 2021-11-30 | 红河弘毅农业发展有限责任公司 | Tobacco planting biodegradable mulching film capable of improving soil performance and preparation method thereof |
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Application publication date: 20120425 |