CN101200387A - Preparation of microorganium fertilizer by using bacilluslaterosporus and bacillus subtilis - Google Patents
Preparation of microorganium fertilizer by using bacilluslaterosporus and bacillus subtilis Download PDFInfo
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- CN101200387A CN101200387A CNA200610022485XA CN200610022485A CN101200387A CN 101200387 A CN101200387 A CN 101200387A CN A200610022485X A CNA200610022485X A CN A200610022485XA CN 200610022485 A CN200610022485 A CN 200610022485A CN 101200387 A CN101200387 A CN 101200387A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention discloses a method for preparing microbiological organic fertilizer by utilizing bacillus laterosporus and bacillus subtilis. The components of the microbiological organic fertilizer are: livestock manure of 25 percent to 30 percent, kitchen garbage of 10 percent and 15 percent, humic acid of 15 percent to 20 percent, fungus chaff of 15 percent to 20 percent, phosphate fertilizer of 5 percent to 15 percent, potash fertilizer of 5 percent to 10 percent, straw of 10 percent to 15 percent, microorganism of 3 percent to 7 percent and trace element of 1 percent to 1.5 percent. The microorganism specifically comprises the bacillus laterosporus and the bacillus subtilis. The preparation process is that: 1. the bacillus laterosporus and the bacillus subtilis are planted at a PDA culture medium inclined plane for making bacterium suspension, and then are sprayed at a carrier to produce spore powder. 2. the livestock manure of 25 percent to 30 percent, kitchen garbage of 10 percent and 15 percent, soil carbon of 15 percent to 20 percent, fungus chaff of 15 percent to 20 percent, straw powder of 10 percent to 15 percent, lemery salt of 5 percent to 10 percent, monocalcium phosphate and ordinary superphosphate of 5 percent to 15 percent, calcium-magnesia phosphate fertilizer of 3 percent to 5 percent, boric sludge of 5 percent to 10 percent, green copperas of 0.5 percent to 1 percent, plaster of 1 percent to 2 percent and lime of 1 percent to 1.5 percent are mixed round equably, and the moisture is adjusted to 55 percent to 60 percent, and then is arranged into a fermenter. Simultaneously the ingredients are added with enzyme microorganism, and are turned in mechanization, so that the materials can be loosened for increasing oxygen. After being fermented for about 25 days, the ingredients can be dried and crushed into finished product for being packed.
Description
Technical field:
The present invention relates to a kind of microorganism organic fertilizer and preparation method thereof.
Background technology:
Whole world agriculture production just develops towards the direction of Organic farming, green agriculture, and this is a Development Trend of World Agriculture, also is the outlet of Agricultural Sustainable Development in China.Be engaged in organic grow, green agricultural product production, using microorganism organic fertilizer is obligato basis.After the China's entry into the WTO, can food safety, food nutrition still march on towards the problem that the Chinese common people of well-off society pay close attention to the most comprehensively, are that can the agricultural-food of large agricultural country China go to the world yet, incorporate the key of world commerce mighty torrent.But, long-term a large amount of use of traditional chemical fertilizer in agriculture production, though make the output of agricultural-food be greatly improved, but employed chemical fertilizer has 70% loss approximately or is fixed in the soil, cause soil compaction, environmental pollution, infringement human health, because soil long-term lacking organic matter causes the crop products quality to descend, seriously restricting the Sustainable development of agricultural simultaneously.Addressing these problems the most basic way is usage quantity, raising chemical fertilizer utilization ratio, the increase soil organism that reduces chemical fertilizer.Because the most fertilizers that use of China peasant still are traditional composting method, the composting time is long, fertilizer efficiency is low, contaminate environment, and the fertilizer that does not fully become thoroughly decomposed still is the main contagium that farm crop are propagated disease, insect pest, makes the especially disease and pest aggravation of greenhouse crops of farm crop.Therefore, thereby can improve quality of agricultural product after microorganism organic fertilizer of the present invention is used, improve Soil structure, the utilization ratio that improves chemical fertilizer reduces the chemical fertilizer usage quantity, reduces disease and pest, increasing both production and income, this has created the huge market space to microbial organic fertilizer.
Summary of the invention:
The object of the invention is to design a kind of microorganism organic fertilizer, and this fertilizer is that animal dung, meal cupboard rubbish, bacterium slag, stalk, humic acids, phosphorus, potassium, multiple microbial bacteria and trace element etc. are composited under specific micro-aerobe fermentation processing condition by a certain percentage.It is used for farm crop, can play and improve agricultural product quality, the structure of improving the soil, raising chemical fertilizer utilization ratio, the effect of reduce the harm of disease worm, increasing the yield per unit area.
By design of the present invention, the composition of this microorganism organic fertilizer is: animal dung 25-30%, meal cupboard rubbish 15-20%, humic acids 15-20%, bacterium slag 15-20%, phosphate fertilizer 5-15%, potash fertilizer 5-10%, stalk 10-15%, multiple microbial bacteria 2-8%, micro-1-1.5% measure by weight percentage.
Each composition role is below described:
1, animal dung: animal dung comprises animal manure and brid guano two big classes.The livestock fecaluria is meant the movement of raise livestocks such as pig, horse, ox, sheep, and it contains abundant organic matter and various nutritive element.Brid guano is the movement of poultry such as chicken, duck, goose, and it contains abundant organic matter and nitrogen, phosphorus, potassium and other nutrition.Animal dung is behind microbial fermentation, and Xiang Benfei provides abundant organic matter and nutritive element.
2, meal cupboard rubbish: meal cupboard rubbish is the domestic refuse that food and drink departments such as hotel, restaurant are produced, it contains a large amount of plant quasi-greases, animal quasi-grease, Mierocrystalline cellulose, bone cutin class, behind microbial fermentation, Xiang Benfei provides abundant organic matter and lipid acid.
3, humic acids: humic acids is the animals and plants remains, mainly is the remains of plant, through decomposition and the conversion of microorganism, and a series of chemical process and a type organic matter that accumulates.It is the polymeric organic acid that is made of aromatic series and multiple functional group thereof, has functions such as good physiologically active and absorption, complexing, exchange, plays the effect of the structure of improving the soil.It and inorganic phosphorus, potassium element chelating form phosphorus humic acid phosphorus, potassium humate, are the important components of organic fertilizer.
4, bacterium slag: the waste that is left behind the edible fungus culturing provides organism such as a large amount of mycoprotein, amino acid, VITAMIN, organic acid for this fertilizer.
5, phosphate fertilizer, potash fertilizer: inorganic phosphorus, potassium element chelating form phosphorus humic acid phosphorus, potassium humate, are the important components of organic fertilizer.
5, microbial bacteria: side gemma spore bacillus (Bacillus Laterosporus), Bacillus subtillis (Bacillus Subtilis), these two kinds of microbial bacterias survive in the microorganism organic fertilizer with the statospore form, have high temperature resistant, salt tolerant branch, anti-ridity unique effects.Microbial bacteria enters and produces a large amount of extracellular enzymes behind the soil growth and breeding and decompose organic matter in the soil, nutrition directly is provided for crop; Meta-bolites not only can stimulate plant growth, and can also play prevention and elimination of disease and pests; A large amount of beneficial microorganisms occupies advantage at the crop rhizosphere ecosystem, and harmful microorganism is played the effect of " competition suppresses ", helps the healthy and strong growth of crop.
The present invention is realized by following three step technical schemes:
1, the preparation method of side gemma spore bacillus, Bacillus subtillis spore powder
1.1 primary inclined plane is cultivated: Side spore genus bacillus, subtilis are inoculated on the inclined-plane of PDA substratum 30 ℃ and cultivate after 24 hours and make bacteria suspension.
1.2 secondary seed is cultivated: in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion to 1% inoculum size, cultivates 48-72 hour for 25-30 ℃ with the bacterial suspension inoculation for preparing in proportion.
1.3 the preparation of spore powder: cultured seed liquid is sprayed on the carrier, and bake drying packages spare below 80 ℃.Detect gemma quantity, contain the gemma number at 20-70 hundred million/gram.
1.4 the side gemma spore bacillus, the Bacillus subtillis spore powder sample that prepare are deposited in Microbiological Lab of the biological company limited of Sichuan Ai Mengai.
2, the preparation method of organic fertilizer
2.1 raw material is prepared:
2.1.1 animal dung: comprise that chicken and duck excrement, pig cow dung etc. dehydrate.
2.1.2 changing food waste: the solid-liquid separation dry packing is standby.
2.1.3 stalk: be ground into length and be 0.5-2 centimetre straw powder.
2.1.4 bacterium slag, peat: the bacterium slag with the spice machine break up drying, peat is standby with being ground into 40-60 order after drying.
2.1.5 mineral manure is prepared: buy vitriolate of tartar, mono-calcium phosphate and calcium superphosphate, fused(calcium magnesium)phosphate, boron mud, ferrous sulfate, lime, gypsum etc.
2.2 preparation of raw material: mix thoroughly by animal dung 25-30%, changing food waste 10-15%, peat 15-20%, bacterium slag 15-20%, straw powder 10-15%, vitriolate of tartar 5-10%, mono-calcium phosphate and calcium superphosphate 5-15%, fused(calcium magnesium)phosphate 3-5%, boron mud 5-10%, ferrous sulfate 0.5-1%, gypsum 1-2%, lime 1-1.5% batching, adjusting contains moisture in the 55-60% scope, the fermenter fermentation.
2.3 fermentation: will get above-mentioned respectively material ready and put into fermenter, and evenly add enzymatic microorganism simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen.One time fermentation requirement 12-15 days, the fermentation top temperature must reach 65-75 ℃ of degree.Secondary Fermentation needs 5-7 days micro-aerobe fermentations, and temperature is controlled at 48-52 ℃ of degree.
2.4 oven dry, pulverizing: the material that ferments is dried through fluidized-bed, and temperature is controlled at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches the 60-80 order, and is standby.
3, microorganism organic fertilizer preparation
The Side spore genus bacillus for preparing, subtilis spore powder (weight ratio 1: 1) are added in the organic fertilizer in the ratio of 3-7%, guarantee total viable count reach 2,000 ten thousand/more than the gram, each bacterium number of viable reach 1,000 ten thousand/more than the gram.Fully mix canned one-tenth commodity microbial organic fertilizer thoroughly at stirrer.
Claims (2)
1. the component of this microorganism organic fertilizer is: animal dung 25-30%, meal cupboard rubbish 15-20%, humic acids 15-20%, bacterium slag 15-20%, phosphate fertilizer 5-15%, potash fertilizer 5-10%, stalk 10-15%, microbial bacteria 3-7%, micro-1-1.5%, amount by weight percentage.
2. microbial bacteria according to claim 1 refers in particular to: 1. bacillus laterosporus (Bacillus Laterosporus), 2. subtilis (Bacillus Subtilis).
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| CN200610022485XA CN101200387B (en) | 2006-12-13 | 2006-12-13 | Preparation of microorganium fertilizer by using bacilluslaterosporus and bacillus subtilis |
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| WO2018096429A1 (en) | 2016-11-22 | 2018-05-31 | Solyom Zoltan | Procedure for preserving soil bacteria, suitably to produce preserved soil-improving bio-fertilizers |
| CN107162772A (en) * | 2017-06-22 | 2017-09-15 | 合肥永泰新型建材有限公司 | It is a kind of for fertilizer of vegetable cultivation and preparation method thereof |
| CN107382585A (en) * | 2017-07-26 | 2017-11-24 | 合肥梵腾环保科技有限公司 | A kind of rice environment-friendly fertilizer and preparation method thereof |
| RU2667159C1 (en) * | 2017-09-05 | 2018-09-17 | Екатерина Олеговна Башмакова | Organomineral fertiliser |
| CN108358681A (en) * | 2018-01-20 | 2018-08-03 | 兴国县丰硒农业发展有限责任公司 | A kind of biological organic fertilizer |
| CN108191577A (en) * | 2018-03-06 | 2018-06-22 | 哈尔滨金农科生物科技开发有限公司 | There is the middle micro- long-acting complex fertilizer of the adaptation blackland of soil improvement |
| CN110713424A (en) * | 2019-11-06 | 2020-01-21 | 横县振茂茶厂 | Method for preparing special fertilizer for jasmine by taking tea-hair and jasmine flower residues as raw materials |
| CN111662132A (en) * | 2020-06-12 | 2020-09-15 | 上海大龙现代农业科技有限公司 | Special biological organic fertilizer with weeding function for rice field and preparation method thereof |
| CN113083869A (en) * | 2021-04-29 | 2021-07-09 | 沈阳禾康永丰生物环保产业有限公司 | Kitchen waste treatment method |
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