CN103965916A - Soil restoration agent, method for preparing soil restoration agent and method for restoring soil - Google Patents
Soil restoration agent, method for preparing soil restoration agent and method for restoring soil Download PDFInfo
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Abstract
The invention discloses a soil restoration agent, which comprises bacilluslaterosporus spore powder, bacillus subtilis spore powder, organic fertilizer, peat and sepiolite. Wherein more than 2*107 living bacteria are contained in each gram of the soil restoration agent; the living bacteria of the bacillus laterosporus account for 40 to 60% of the total living bacteria; and the living bacteria of the bacillus subtilis also account for 40 to 60% of the total living bacteria. The soil restoration agent provided by the invention can help soil form a good biosphere, and has the effects of degrading heavy metals, activating soil, improving thestructure of soil, retaining water and fertilizer, and improving the effect of the fertilizer. Meanwhile, the soil restoration agent further increases soil humus and living microorganism, reduces harm of pests and diseases and improves the yield per unit area and the quality of agricultural products.
Description
Technical field
The present invention relates to a kind of soil-repairing agent and preparation method thereof and the soil remediation method that uses this soil-repairing agent.
Background technology
Soil pollution is subject to the extensive concern of countries in the world just day by day as the problem of a restriction human social.Cause the reason of soil pollution to mainly contain excessive use chemical fertilizer, chemical pesticide, the improper disposal of various sewage, mud and organic waste, the accidental release of objectionable impurities and the long-term accumulation of each pollutant in soil etc.Soil pollution causes time pollution to underground water, surface water, enters human body jeopardize human health by tap water or soil-botanical system via food chain.
Soil remediation is to make to suffer contaminated soil to recover the technical measures of normal function, refer to and utilize physics, chemistry and biological method shift, absorb, degrade and transform the pollutent in soil, make its concentration be reduced to acceptable level, or poisonous and hazardous pollutent is converted into harmless material.Basically, the know-why of contaminated soil reparation can be included as: (1) changes pollutent and have form or a combination with soil in soil, reduces its transportable property and bioavailability in environment; (2) concentration of objectionable impurities in reduction soil.
The recovery technique research of contaminated soil becomes the emphasis of current environmental engineering Science and Technology research; conventional contaminated soil restorative procedure mostly is physics and chemistry method at present; its advantage is directly, fast; but long-lasting is poor; using method or consumption are improper; not only do not reach repairing effect, occur on the contrary new pollution.Adopt microbial technique to add organic processing, can reach holding effect, comprehensive, comprehensive effect, can not bring any detrimentally affect.
Therefore, develop that a kind of long-lasting is strong, comprehensive effectiveness is high, without any dysgenic biotype soil remediation material, basic place of the present invention just.
Summary of the invention
The object of the invention is with a kind of biological organic type soil-repairing agent of modern biotechnology exploitation, thereby reach the effect that effective improvement improves useful living microorganism flora and organic matter, raising Fertilizer application effect, the ability that strengthens crop disease-resistant insect pest, the raising quality of agricultural product in heavy metal, the increase soil in soil aggregate, degraded soil and increases agricultural output.
Another object of the present invention is the preparation method for a kind of soil-repairing agent is provided.
Another object of the present invention is for a kind of soil remediation method is provided.
The invention provides a kind of soil-repairing agent, wherein, described soil-repairing agent comprises: bacillus laterosporus (Bacillus Laterosporus) spore powder, subtilis (Bacillus Subtilis) spore powder, organic fertilizer, peat and sepiolite, and every gram of contained viable count of described soil-repairing agent is 2 × 10
7individual, wherein, the 40-60% that the viable count of described bacillus laterosporus is total viable count, the 40-60% that the viable count of described subtilis is total viable count.
The present invention also provides the preparation method of described soil-repairing agent, wherein, the method comprises bacillus laterosporus spore powder, subtilis spore powder, organic fertilizer, peat is contacted and mixed with sepiolite, make gross weight taking described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5%, and the content of subtilis spore powder is 2-2.5%, and the content of organic fertilizer is 30-35%, the content of peat is 40-45%, and the content of sepiolite is 20-25%.
The present invention also provides a kind of soil remediation method, and wherein, the method comprises soil-repairing agent of the present invention is applied in soil to be repaired.
Soil-repairing agent provided by the invention can help soil to form good biosphere, play degraded heavy metal, activating soil, improve the effect of Soil structure, water conservation, fertilizer conservation, raising fertilizer efficiency, have increases agron and living microorganism, the sick worm harm of minimizing, increases the yield per unit area and effect of agricultural product quality simultaneously.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
The invention provides a kind of soil-repairing agent, wherein, described soil-repairing agent comprises: bacillus laterosporus (Bacillus Laterosporus) spore powder, subtilis (Bacillus Subtilis) spore powder, organic fertilizer, peat and sepiolite, and viable count contained in described soil-repairing agent is greater than 2 × 10
7individual/gram, wherein, the 40-60% that the viable count of described bacillus laterosporus is total viable count, the 40-60% that the viable count of described subtilis is total viable count.
In the present invention, taking the gross weight of described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5 % by weight, the content of subtilis spore powder is 2-2.5 % by weight, the content of organic fertilizer is 30-35 % by weight, the content of peat is 40-45 % by weight, and the content of sepiolite is 20-25 % by weight.Under preferable case, taking the gross weight of described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2.1-2.3 % by weight, the content of subtilis spore powder is 2.1-2.3 % by weight, the content of organic fertilizer is 31-33 % by weight, the content of peat is 42-44 % by weight, and the content of sepiolite is 21-23 % by weight.
In the present invention, the bacterial classification of described bacillus laterosporus (Bacillus Laterosporus), subtilis (Bacillus Subtilis) all can be commercially available.
In the present invention, bacillus laterosporus and subtilis spore are present in soil-repairing agent with the form of dormant state, and that the bacterium of spore form has is high temperature resistant, salt tolerant divides, siccostabile characteristic.In the time that soil-repairing agent of the present invention is applied in soil, microbial spores is Fast Growth breeding at suitable moisture, temperature, the extracellular enzyme of its generation can decompose the organic matter in soil, nutrition is directly provided to crop, its meta-bolites has the effect that stimulates meta-bolites, can effectively prevent and treat or reduce the harm of disease worm, contribute to the stalwartness growth of crop.
In the present invention, described peat is atmospheric swamp ground product, nontoxic, pollution-free, contain very high organic matter, humic acid and nutritive ingredient, there is good loosening, ventilative, water-permeable, water conservation, fertilizer-preserving ability is strong, quality is light, in renovation agent provided by the present invention mainly as matrix, effect comprises increases organic matter, the nutritive ingredients such as humic acid, activating soil, degraded heavy metal, create microbial activities environment, wherein, be preferably 60-65 % by weight taking the gross weight of peat as the organic content of benchmark peat used in the present invention, content of humic acid is 35-40 % by weight, the particle diameter of peat is 40-60 order.
In the present invention, described sepiolite be have chain-like structure containing Shuifu County's magnesium silicate clay mineral.There is the performances such as shrinking percentage is low, plasticity-good, surface is large, adsorptivity is strong.Sepiolite in soil-repairing agent provided by the present invention mainly as the support material of soil disinfection, it act as the comprehensive utilization ratio of improving Soil structure, water conservation, fertilizer conservation, raising fertilizer, preferably, the particle diameter of sepiolite used in the present invention is 80-100 order.
In the present invention, the kind of described organic fertilizer can in very large range change, and the organic fertilizer that can be feces of livestock and poultry and plant material obtain through fermentation, taking the gross weight of organic fertilizer as benchmark, organic content is 35-50 % by weight, and the content of moisture is 25-30 % by weight.In described organic fertilizer, in nitrogen element, the content of nitrogen is 1-3 % by weight; In phosphoric, the content of phosphorus is 0.5-2 % by weight, in potassium element, and the content of potassium is 1-3 % by weight.Described livestock and poultry can be one or more in livestock and poultry, and described plant material can be agricultural crop straw.
In the present invention, the organic fertilizer that described organic fertilizer can be commercially available can be also the fertilizer preparing by this area ordinary method, and preferred, in the present invention, the particle diameter of fertilizer is 40-60 order.
Soil-repairing agent provided by the present invention can also contain other auxiliary materials, and wherein, taking the gross weight of described renovation agent as benchmark, the content of auxiliary material is 2-5 % by weight; Described auxiliary material can be trace element, for example, can be magnesium, molybdenum, zinc etc.
The present invention has no particular limits for the scope of using of soil-repairing agent, and can be applied to all kinds ofly needs to improve crumb structure, the heavy metal of degrading, increases the soil of useful living microorganism flora and organic content.
The present invention also provides the preparation method of described soil-repairing agent, wherein, the method comprises bacillus laterosporus spore powder, subtilis spore powder, organic fertilizer, peat is contacted and mixed with sepiolite, make gross weight taking described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5 % by weight, the spore powder content of subtilis is 2-2.5 % by weight, the content of organic fertilizer is 30-35 % by weight, the content of peat is 40-45 % by weight, and the content of sepiolite is 20-25 % by weight.
In the present invention, the method comprises bacillus laterosporus and subtilis is inoculated in respectively and in substratum, cultivates preparation spore powder, the method of preparing bacillus laterosporus and subtilis in the present invention can in very large range change, and the method can comprise: independently cultivating respectively bacillus laterosporus and subtilis in culture system separately; Obtaining gemma number in every gram of spore powder is the bacillus laterosporus of hundred million of 80-100, and to obtain gemma number in every gram of spore powder be the subtilis of hundred million of 80-100, and the bacillus laterosporus obtaining respectively, subtilis are proportionally mixed.Wherein, the 40-60% that the viable count of described bacillus laterosporus is total viable count, the 40-60% that the viable count of described subtilis is total viable count.
In the present invention, the cultural method of described bacillus laterosporus and subtilis is conventionally known to one of skill in the art, and for example, preparation process can specifically be carried out according to following steps:
Primary inclined plane is cultivated: bacillus laterosporus, subtilis are inoculated on the inclined-plane of PDA substratum at 30 DEG C and make bacteria suspension after cultivating 24 hours.
Secondary seed cultivate: in the inoculum size of 1% ratio by the bacterial suspension inoculation preparing in PDA liquid nutrient medium, concussion cultivate (shaking table revolution is 160 revs/min), 25-30 DEG C cultivate 48-72 hour.
The preparation of spore powder: cultured seed liquor is sprayed to carrier (this carrier can be Semen Maydis powder, analysis for soybean powder) upper, 80 DEG C of following bake dryings are for subsequent use.Detect spore quantity reach 80-10 hundred million/gram.
In the present invention, the formula that consists of conventional medium known in those skilled in the art of PDA substratum, and can be commercially available.
In the present invention, peat, sepiolite and organic fertilizer can obtain by the mode being purchased.
In the present invention, bacillus laterosporus, subtilis, peat, sepiolite and organic fertilizer are contacted to the mode mixing to be had no particular limits, as long as in the soil-repairing agent that can make to obtain, make gross weight taking described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5 % by weight, the spore powder content of subtilis is 2-2.5 % by weight, the content of organic fertilizer is 30-35 % by weight, the content of peat is 40-45 % by weight, and the content of sepiolite is 20-25 % by weight.
The present invention also provides a kind of soil remediation method, and wherein, the method comprises soil-repairing agent of the present invention is applied in soil to be repaired.
In the present invention, the method of application of described soil-repairing agent can change within a large range, it can be the method for application of soil-repairing agent known in those skilled in the art, as long as can make the soil for 100 square metres, the amount of application of described soil-repairing agent is 150-230kg, for example, application can carry out according to whole layer placement of fertilizer mode:
Below will describe the present invention by embodiment.In following examples,
Bacillus laterosporus bacterial strain is purchased from Ministry of Agriculture's country's microniological proudcts inspection center.
Bacillus subtilis strain is purchased from Ministry of Agriculture's country's microniological proudcts inspection center.
In peat, organic content is 62 % by weight, and content of humic acid is 32 % by weight, and particle diameter is 55 orders, purchased from Sichuan Aimengai Bio-technology Co., Ltd, and trade mark He Dao.
The particle diameter of sepiolite is 100 orders, and purchased from Huozhou, Shanxi petrel Nong Hua company limited, the trade mark is petrel.
Organic fertilizer is prepared in the following manner:
Raw material is prepared:
Animal dung: chicken and duck excrement, pig cow dung are dehydrated;
Stalk: it is the straw powder of 0.5-2 centimetre that corn straw smashing is become to length;
Enzymatic microorganism: purchased from Zhao De bio tech ltd, Beijing, the trade mark is million morals;
Preparation of raw material: according to animal dung 85 % by weight, straw powder 12 % by weight, the ratio of enzymatic microorganism 3 % by weight is mixed batching to regulate moisture content in 60 % by weight thoroughly, and the mixture of acquisition ferments to fermenter;
One time fermentation: the mixture that upper step is obtained carries out mechanize and turns over throwing, with opening material, increase amount of oxygen, one time fermentation carries out 15 days, and fermentation top temperature reaches 72 DEG C;
Secondary Fermentation: adopt buttress formula to store up the material of one time fermentation and carry out secondary micro-aerobe fermentation in 25 days, temperature is controlled at 48-52 DEG C;
Dry, pulverize: material good Secondary Fermentation is dried through tunnel kiln drying, and temperature is controlled at 80 DEG C below degree, and water content is controlled within the scope of 20-25%, pulverizes and sieves, and fineness reaches 50 orders, stores for future use;
Preparation example 1-4
Preparation example 1-4 is for illustrating the preparation method of soil-repairing agent provided by the present invention.
Bacillus laterosporus, subtilis, peat, sepiolite, organic fertilizer are fully mixed thoroughly by the incorgruous screw mixer of twin columns according to ratio listed in table 1, made renovation agent 1-4.
Table 1
Test case 1-4
Test case 1-4 is for illustrating using method and the effect of soil-repairing agent provided by the present invention.
By preparation example 1-4, the renovation agent 1-4 making is applied to respectively different areas, different soils according to the listed consumption of table 2 by ring layer fertilizing method:
Table 2
| Renovation agent | Trial site | Soil type | Consumption (kg/m 2) | Application process | Net effect |
| 1 | Dujiang weir, Sichuan | Loam | 2.3 | Holostrome | 92％ |
| 2 | Maerkang, Sichuan | Sand | 1.8 | Holostrome | 91％ |
| 3 | Hainan Le Dong | Laterite | 1.5 | Holostrome | 86％ |
| 4 | Lulong, Hebei | Loam | 2.3 | Holostrome | 90％ |
Net effect: the variation of soil organic matter content before and after referring to use, nutrient, granule, active bio flora, harmful bacteria, induced worm egg death rate, heavy metal index.The wherein organic matter in soil and N+P
2o
5+ K
2o nutrient content is pressed the method for NYT525-2002 prescribed by standard and is measured; Living microorganism bacterium is pressed the method for NYT798-2004 prescribed by standard and measures; Pernicious bacteria number is pressed the method for defined in GB/T19524.1--2004 and is measured; The mensuration of pressing defined in GB/T19524.2-2004 of induced worm egg death rate; As, Cd, Pb, Cr, Hg heavy metal index are pressed in GB18877-2002 method of defined in 5.12～5.17 and are measured; Soil granular is judged with physical method.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.It should be noted that in addition, each concrete technical characterictic described in above-mentioned embodiment, in reconcilable situation, can combine for fear of unnecessary repetition by any suitable mode, the present invention is to the explanation no longer separately of various possible array modes.
In addition, also can carry out arbitrary combination between various embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a soil-repairing agent, it is characterized in that, described soil-repairing agent comprises: bacillus laterosporus (Bacillus Laterosporus) spore powder, subtilis (Bacillus Subtilis) spore powder, organic fertilizer, peat and sepiolite, and viable count contained in described soil-repairing agent is greater than 2 × 10
7individual/gram, wherein, the 40-60% that the viable count of described bacillus laterosporus is total viable count, the 40-60% that the viable count of described subtilis is total viable count.
2. soil-repairing agent according to claim 1, wherein, taking the gross weight of described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5 % by weight, the content of subtilis spore powder is 2-2.5 % by weight, the content of organic fertilizer is 30-35 % by weight, and the content of peat is 40-45 % by weight, and the content of sepiolite is 20-25 % by weight.
3. soil-repairing agent according to claim 1, wherein, taking the gross weight of peat as benchmark, the organic content in described peat is 60-65 % by weight, and content of humic acid is 35-40 % by weight, and the particle diameter of peat is 40-60 order.
4. soil-repairing agent according to claim 1, wherein, the particle diameter of described sepiolite is 80-100 order.
5. according to the soil-repairing agent described in any one in claim 1-4, wherein, described organic fertilizer is the fertilizer that feces of livestock and poultry and agricultural crop straw obtain through fermentation.
6. the preparation method of the soil-repairing agent described in claim 1-5, it is characterized in that, the method comprises bacillus laterosporus spore powder, subtilis spore powder, organic fertilizer, peat is contacted and mixed with sepiolite, make gross weight taking described renovation agent as benchmark, the content of bacillus laterosporus spore powder is 2-2.5 % by weight, the spore powder content of subtilis is 2-2.5 % by weight, the content of organic fertilizer is 30-35 % by weight, the content of peat is 40-45 % by weight, and the content of sepiolite is 20-25 % by weight.
7. the preparation method of soil-repairing agent according to claim 6, wherein, the method comprises: preparing gemma number in every gram of spore powder at inoculation of medium bacillus laterosporus is the bacillus laterosporus spore powder of hundred million of 80-100, and to prepare gemma number in every gram of spore powder inoculation of medium subtilis be the subtilis spore powder of hundred million of 80-100.
8. the preparation method of soil-repairing agent according to claim 6, wherein, taking the gross weight of organic fertilizer as benchmark, organic content is 35-50 % by weight, the content of moisture is 25-30 % by weight.
9. a soil remediation method, is characterized in that, the method comprises the soil-repairing agent described in claim 1-5 is applied in soil to be repaired.
10. soil remediation method according to claim 9, wherein, with respect to the soil of 100 square metres, the amount of application of described soil-repairing agent is 150-230kg.
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| CN105347884A (en) * | 2015-11-20 | 2016-02-24 | 河北慈心环保科技有限公司 | Biological organic repairing agent for soil pollution and application and preparation method thereof |
| CN105921509A (en) * | 2016-04-29 | 2016-09-07 | 山东农大肥业科技有限公司 | Soil heavy metal treating agent and application thereof |
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| CN104311336A (en) * | 2014-10-21 | 2015-01-28 | 苗庆龄 | Soil restorative agent for treating heavy metal pollution and preparation process of soil restorative agent |
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