CN102153387A - Method for preparing brown nitrogen-fixing bacteria microbial bacterial fertilizer - Google Patents
Method for preparing brown nitrogen-fixing bacteria microbial bacterial fertilizer Download PDFInfo
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- CN102153387A CN102153387A CN2011101229067A CN201110122906A CN102153387A CN 102153387 A CN102153387 A CN 102153387A CN 2011101229067 A CN2011101229067 A CN 2011101229067A CN 201110122906 A CN201110122906 A CN 201110122906A CN 102153387 A CN102153387 A CN 102153387A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for preparing a brown nitrogen-fixing bacteria microbial bacterial fertilizer, which comprises the following steps of: preparing brown nitrogen-fixing bacteria into 60 to 80 hundred million/ml bacteria suspension; then mixing solid farmyard manure, straw powder, adsorbent and calcium superphosphate or potassium dihydrogen phosphate, and fermenting the mixture by adding water to obtain a decomposed material; and finally, mixing the decomposed material, sugar, sterile water and the bacteria suspension uniformly, fermenting and drying. The effect of the bacterial fertilizer is high; the number of the beneficial bacteria reaches 151 hundred million per gram, wherein the number of the brown nitrogen-fixing bacteria can reach 7*10<8> per gram; and the bacterial fertilizer can effectively increase the yield of crops, is convenient to apply, and is particularly suitable for gramineous pasture, for example, the annual yield of ryegrass reaches 5,000 to 5,500kg/667m<2>.
Description
Technical field
The present invention relates to the microbial-bacterial fertilizer technical field, particularly relate to a kind of preparation method of azotobacter vinelandii microbial-bacterial fertilizer.
Background technology
Azotobacter vinelandii is easy to cultivate, and can fix a large amount of nitrogens in the laboratory, so use this to belong to bacterium to the research of fixed nitrogen more.At occurring in nature, azotobacter vinelandii is distributed widely in soil and the water, can fix free nitrogen and has vital role, generally retarded growth in the slant acidity environment.Azotobacter vinelandii is as useful vinelandii, isolated condition is energy self fixed nitrogen down, strong (the culture presevation system handbook of nitrogen fixing capacity, Institute of Microorganism, Academia Sinica, Science Press, 1980), if be made into bacterial manure, can not only in soil and various crop rhizosphere, so that being provided, the nitrogen nutrition demand of crop can stimulate plant growth by secreting hormone again by the nitrogen in the fixed air.
Summary of the invention
Technical problem to be solved by this invention provides a kind of microbial-bacterial fertilizer, contains a large amount of azotobacter vinelandiis and probiotics in this bacterial manure, can fix a large amount of airborne nitrogens, is made for the thing growth.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme:
The preparation method of azotobacter vinelandii microbial-bacterial fertilizer of the present invention comprises the steps:
(1) preparation of bacteria suspension: the bacteria suspension that azotobacter vinelandii is mixed with 60～8,000,000,000/ml;
(2) fermentation maturity: according to listed as parts by weight, get 60～70 parts of solid farm manures, add 20～30 parts of straw powder, 4～6 parts in sorbent material, 2～3 parts of calcium superphosphate or 1.5～2.5 parts of potassium primary phosphates, 1～2 part in water, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
(3) preparation bacterial manure: according to listed as parts by weight, get 90～95 parts in the material that becomes thoroughly decomposed, 0.5～1.5 part of sugar, 5～7 parts of aqua sterilisas, bacteria suspension 0.3～1-2 part, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
Further, above-mentioned preparation method is:
(1) preparation of bacteria suspension: the bacteria suspension that azotobacter vinelandii is mixed with 6,800,000,000/ml;
(2) fermentation maturity: according to listed as parts by weight, get 65 parts of solid farm manures, add straw or 25 parts of straw powder, weathered coal or 5 parts in careless carbon, 2.5 parts of calcium superphosphate or 2 parts of potassium primary phosphates, 1.5 parts in water, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
(3) preparation bacterial manure: according to listed as parts by weight, get 92.6 parts in the material that becomes thoroughly decomposed, 1 part of sugar, 6 parts of aqua sterilisas, 0.4 part of bacteria suspension, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
Preferably, used bacteria suspension prepares as follows among the aforementioned preparation method:
(1) enrichment culture liquid preparation: sucrose 15g, potassium primary phosphate 0.8g, sal epsom 0.2g, sodium-chlor 0.2g, lime carbonate 1g, Sodium orthomolybdate 0.05g, ferrous sulfate 0.1g, peptone 5g, water 1000ml, pH are 6.5～7.0;
(2) enrichment culture method: get 3～5g rich soil soil sample and make muddy liquid with 50ml water, absorption 5ml suspension is packed into and is filled in the 250ml triangular flask of 30ml enrichment culture liquid, and 150rpm, cultivated 3～5 days by 25～30 ℃;
(3) preparation of plate isolation substratum: in enrichment culture liquid, add 2% agar and make the plate isolation substratum, sterilization, standby;
(4) plate isolation is cultivated: get the nutrient solution after the enrichment culture, coat the plate isolation substratum after 10 times of dilutions, cultivated 2 days for 28 ℃;
(5) increasing bacterium cultivates: get the flat-plate bacterial colony of (4) gained, move into enrichment culture liquid, cultivated 2 days for 28 ℃, get bacterium liquid;
(6) fermentation: by enrichment culture liquid 100: the weight part ratio of bacterium liquid 3～7 is mixed, and places fermentor tank, i.e. shaker fermentation cultivation, 200 rev/mins of stirring velocitys, 27 ± 1 ℃ of culture temperature were cultivated 24 hours, then at 29 ± 1 ℃ of jar temperature, tank pressure 0.4～0.5kg/cm
3, 180 rev/mins of stirring velocitys condition under cultivated 48 hours, collect fermented liq, regulate pH 6.8～7.2, stir, get final product.
Adopt the microbial-bacterial fertilizer of technique scheme preparation, have good effect of increasing production:
(1) place to use
Guizhou Farming Animal Science and Veterinary Research Institute is main observation place with the Longli County experimental field, establishes three processing: azotobacter vinelandii microbial-bacterial fertilizer, farm manure and not fertilising.Apply the place and be mainly counties and cities such as Longli, Tianzhu, Danzhai and Bijie.
(2) seeding and fertilizing
In March, 2010 and in March, 2011, the field test total area 1200 ㎡, 1000 mu of extend trial areas.The azotobacter vinelandii microbial-bacterial fertilizer applies by 40～60 ㎏/667 ㎡ consumptions, farm manure (base manure) 540 ㎏/667 ㎡, and successively twice equivalent applies, other Routine Management unanimity.Perhaps by seed: microbial-bacterial fertilizer is the weight ratio inoculation of 10:1.Should avoid the sunlight direct projection during microbe inoculation bacterial manure, the seed of having inoculated microbial-bacterial fertilizer should finish day.
By adopting the plantation and the fertilizing method of science, herbage and crop yield are improved, and paddy rice, rye grass, corn, the comparison of vegetables crop yield have improved 4.2%, 6.0%, 4.9%, 4.0% respectively according to singly executing farm manure.
Compared with prior art, the present invention is with agricultural crop straw, the rice husk or the peat composed of rotten mosses are carrier, azotobacter vinelandii is inoculated in farm manure is prepared into microbial-bacterial fertilizer, solved pollution and the agricultural crop straw efficient problem utilized of farming animals ight soil to environment, and the lactobacillus that produces in azotobacter vinelandii and the stockpile fermentation, yeast, Trichoderma, aspergillar interacts feces of livestock and poultry is degraded rapidly, transform, become thoroughly decomposed, also can make material form the micro-ecological environment of suitable beneficial microbe colony growth, beneficial microorganism can be bred rapidly, can produce all kinds of biologically active substances, suppress the growth of harmful bacterium effectively, promote crop vigorous growth, improve crop yield, strengthen the resistance against diseases of farm crop, improve soil fertility, Effect of Bacteria Fertilizer height of the present invention, probiotics number are up to 15,100,000,000/g, and wherein azotobacter vinelandii can reach 7 * 10
8Individual/g, can effectively increase production farm crop, and conveniently use, be specially adapted to graminous pasture, reach 5000～5500 ㎏/667 ㎡ as the rye grass annual output.
Embodiment
Embodiment 1: prepare bacteria suspension at first as follows
(1) enrichment culture liquid preparation: sucrose 15g, potassium primary phosphate 0.8g, sal epsom 0.2g, sodium-chlor 0.2g, lime carbonate 1g, Sodium orthomolybdate 0.05g, ferrous sulfate 0.1g, peptone 5g, water 1000ml, pH are 6.5～7.0;
(2) enrichment culture method: get 3～5g rich soil soil sample and make muddy liquid with 50ml water, absorption 5ml suspension is packed into and is filled in the 250ml triangular flask of 30ml enrichment culture liquid, and 150rpm, cultivated 3～5 days by 25～30 ℃;
(3) preparation of plate isolation substratum: in enrichment culture liquid, add 2% agar and make the plate isolation substratum, sterilization, standby;
(4) plate isolation is cultivated: get the nutrient solution after the enrichment culture, coat the plate isolation substratum after 10 times of dilutions, cultivated 2 days for 28 ℃;
(5) increasing bacterium cultivates: get the flat-plate bacterial colony of (4) gained, move into enrichment culture liquid, cultivated 2 days for 28 ℃, get bacterium liquid;
(6) fermentation: by enrichment culture liquid 100: the weight part ratio of bacterium liquid 3～7 is mixed, and places fermentor tank, i.e. shaker fermentation cultivation, 200 rev/mins of stirring velocitys, 27 ± 1 ℃ of culture temperature were cultivated 24 hours, then at 29 ± 1 ℃ of jar temperature, tank pressure 0.4～0.5kg/cm
3, 180 rev/mins of stirring velocitys condition under cultivated 48 hours, collect fermented liq, regulate pH 6.8～7.2, stir, get final product.
The preparation of bacteria suspension: the bacteria suspension that above-mentioned azotobacter vinelandii is mixed with 6,800,000,000/ml;
Fermentation maturity: get solid farm manure 65kg, add straw powder 25kg, weathered coal (sorbent material) 5kg, calcium superphosphate 2.5kg, water 1.5kg, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
Preparation bacterial manure: get become thoroughly decomposed material 92.6kg, syrup 1kg, aqua sterilisa 6kg, bacteria suspension 0.4kg, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
The effective bacterium number of this azotobacter vinelandii is up to 15,100,000,000/g, and wherein azotobacter vinelandii 7 * 10
8Individual/g.
Embodiment 2:
The preparation of bacteria suspension: get the bacteria suspension that embodiment 1 prepared azotobacter vinelandii is made 6,000,000,000/ml;
Fermentation maturity: get solid farm manure 70kg, add straw powder 30kg, the peat composed of rotten mosses (sorbent material) 4kg, potassium primary phosphate 1.5kg, water 2kg, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
Preparation bacterial manure: get become thoroughly decomposed material 90kg, sucrose 1.5kg, aqua sterilisa 5kg, bacteria suspension 0.3kg, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
Embodiment 3:
The preparation of bacteria suspension: get the bacteria suspension that the azotobacter vinelandii of buying on the market is made 7,000,000,000/ml;
Fermentation maturity: get solid farm manure 60kg, add straw powder 20kg, weathered coal (sorbent material) 6kg, calcium superphosphate 3kg, water 1kg, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
Preparation bacterial manure: get become thoroughly decomposed material 95kg, N.F,USP MANNITOL (sugar) 0.5kg, aqua sterilisa 7kg, bacteria suspension 0.5kg, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
Claims (3)
1. the preparation method of an azotobacter vinelandii microbial-bacterial fertilizer comprises the steps:
(1) preparation of bacteria suspension: the bacteria suspension that azotobacter vinelandii is mixed with 60～8,000,000,000/ml;
(2) fermentation maturity: according to listed as parts by weight, get 60～70 parts of solid farm manures, add 20～30 parts of straw powder, 4～6 parts in sorbent material, 2～3 parts of calcium superphosphate or 1.5～2.5 parts of potassium primary phosphates, 1～2 part in water, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
(3) preparation bacterial manure: according to listed as parts by weight, get 90～95 parts in the material that becomes thoroughly decomposed, 0.5～1.5 part of sugar, 5～7 parts of aqua sterilisas, bacteria suspension 0.3～1-2 part, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
2. according to the preparation method of the described azotobacter vinelandii microbial-bacterial fertilizer of claim 1, it is characterized in that:
(1) preparation of bacteria suspension: the bacteria suspension that azotobacter vinelandii is mixed with 6,800,000,000/ml;
(2) fermentation maturity: according to listed as parts by weight, get 65 parts of solid farm manures, add straw or 25 parts of straw powder, weathered coal or 5 parts in careless carbon, 2.5 parts of calcium superphosphate or 2 parts of potassium primary phosphates, 1.5 parts in water, mix thoroughly, 60～70 ℃ fermented 3～4 days, and promptly got the material that becomes thoroughly decomposed;
(3) preparation bacterial manure: according to listed as parts by weight, get 92.6 parts in the material that becomes thoroughly decomposed, 1 part of sugar, 6 parts of aqua sterilisas, 0.4 part of bacteria suspension, mixing, 25～30 ℃ of fermentations 5～6 days, drying, promptly.
3. according to the preparation method of claim 1 or 2 described azotobacter vinelandii microbial-bacterial fertilizers, it is characterized in that: bacteria suspension prepares as follows:
(1) enrichment culture liquid preparation: sucrose 15g, potassium primary phosphate 0.8g, sal epsom 0.2g, sodium-chlor 0.2g, lime carbonate 1g, Sodium orthomolybdate 0.05g, ferrous sulfate 0.1g, peptone 5g, water 1000ml, pH are 6.5～7.0;
(2) enrichment culture method: get 3～5g rich soil soil sample and make muddy liquid with 50ml water, absorption 5ml suspension is packed into and is filled in the 250ml triangular flask of 30ml enrichment culture liquid, and 150rpm, cultivated 3～5 days by 25～30 ℃;
(3) preparation of plate isolation substratum: in enrichment culture liquid, add 2% agar and make the plate isolation substratum, sterilization, standby;
(4) plate isolation is cultivated: get the nutrient solution after the enrichment culture, coat the plate isolation substratum after 10 times of dilutions, cultivated 2 days for 28 ℃;
(5) increasing bacterium cultivates: get the flat-plate bacterial colony of (4) gained, move into enrichment culture liquid, cultivated 2 days for 28 ℃, get bacterium liquid;
(6) fermentation: by enrichment culture liquid 100: the weight part ratio of bacterium liquid 3～7 is mixed, and places fermentor tank, i.e. shaker fermentation cultivation, 200 rev/mins of stirring velocitys, 27 ± 1 ℃ of culture temperature were cultivated 24 hours, then at 29 ± 1 ℃ of jar temperature, tank pressure 0.4～0.5kg/cm
3, 180 rev/mins of stirring velocitys condition under cultivated 48 hours, collect fermented liq, regulate pH 6.8～7.2, stir, get final product.
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Cited By (3)
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| CN102432361A (en) * | 2011-08-31 | 2012-05-02 | 苏理黔 | Method for producing high-quality active ecological organic raw material from livestock and poultry manure |
| CN103045750A (en) * | 2013-01-16 | 2013-04-17 | 贵州省畜牧兽医研究所 | PCR (Polymerase Chain Reaction) detection kit for azotobacter vinelandii |
| CN108083868A (en) * | 2018-01-19 | 2018-05-29 | 河南省现代农业研究院 | A kind of Organic carbon microbial manure and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432361A (en) * | 2011-08-31 | 2012-05-02 | 苏理黔 | Method for producing high-quality active ecological organic raw material from livestock and poultry manure |
| CN102432361B (en) * | 2011-08-31 | 2013-08-07 | 苏理黔 | Method for producing high-quality active ecological organic raw material from livestock and poultry manure |
| CN103045750A (en) * | 2013-01-16 | 2013-04-17 | 贵州省畜牧兽医研究所 | PCR (Polymerase Chain Reaction) detection kit for azotobacter vinelandii |
| CN103045750B (en) * | 2013-01-16 | 2015-07-29 | 贵州省畜牧兽医研究所 | Azotobacter vinelandii PCR detection kit |
| CN108083868A (en) * | 2018-01-19 | 2018-05-29 | 河南省现代农业研究院 | A kind of Organic carbon microbial manure and preparation method thereof |
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