CN108083868A - A kind of Organic carbon microbial manure and preparation method thereof - Google Patents
A kind of Organic carbon microbial manure and preparation method thereof Download PDFInfo
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- CN108083868A CN108083868A CN201810052377.XA CN201810052377A CN108083868A CN 108083868 A CN108083868 A CN 108083868A CN 201810052377 A CN201810052377 A CN 201810052377A CN 108083868 A CN108083868 A CN 108083868A
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- preparation
- carrier
- bacteria
- microbial manure
- organic carbon
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of Organic carbon microbial manures and preparation method thereof, include the following steps：（1）The preparation of carrier：It is air-dried after impurity larger in maize straw, Chicken dung and peat soil and stone are rejected, takes 1：1：1 parts by weight Corn stalk, Chicken dung and peat soil prepares carrier；（2）The preparation of bacteria suspension：Promoting bacteria is subjected to activation culture in the medium, is prepared into every milliliter of CFU 2 × 108—3×108Bacteria suspension；（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, ratio 2ml/3g is placed 18 days at 20 DEG C after mixing.The present invention is arranged in pairs or groups by rational bacterial strain and carrier, microorganism fertilizer obtained has good adsorption capacity, be conducive to strain existence and Function, and with fixed nitrogen, Soluble phosphorus, potassium decomposing and the bacterial strain of Anti-bacterium effect, pass through the supply of its vital movement increase nutrient for plants.
Description
Technical field
The present invention relates to microbial manure technical fields, and in particular to a kind of Organic carbon microbial manure and its preparation side
Method.
Background technology
Due to the application of a large amount of chemical fertilizer, soil hardening, deteriorating water quality, agricultural product seriously pollute, entire farmland ecological environment
It is affected with the living environment of people, the sustainable development of China's agricultural how to be maintained to cause the height weight of governments at all levels
Depending on.Particularly after China joined WTO, China's agricultural is subject to severe challenge, to develop outlet agriculture oriented for export and foreign exchange earnings, it is necessary to carry forward vigorously green
The development of color food and pollution-free food, and under the impact of international financial crisis, traditional Fertilizer Industry gets deeply stuck in quagmire, newly
Type fertilizer but has good development space, and it is " new " due in new-type fertilizer to trace it to its cause, new scientific and technical, new to apply
Fertile theory and new product advantage.
New-type fertilizer microbial manure as production pollution-free food production data, it has also become Ministry of Agriculture's emphasis promotes production
Product.
Microorganism fertilizer is using domestic waste and agriculture wastes as carrier, is made using the specific function of microorganism
Into.The selection of carrier with the fertilizer efficiency for comparing microorganism fertilizer with being affected.Preferable carrier should have the micropore of a large amount of ordered arrangements,
With the features such as large specific surface area, adsorption capacity be strong, while carrier must be conducive to strain existence and Function, repeat steady
Fixed production, it is nontoxic to crop and soil.But with high content of technology due to microbial manure, production difficulty is big, in city of China
The product of high quality is also seldom on field.
The content of the invention
It is existing micro- to solve the present invention provides a kind of Organic carbon microbial manure and preparation method thereof and preparation method thereof
The problem of bio-feritlizer preparation method method is few.
The purpose of the present invention is what is realized with following technical proposals：
A kind of preparation method of Organic carbon microbial manure, includes the following steps：
（1）The preparation of carrier：It air-dries, takes after impurity larger in maize straw, Chicken dung and peat soil and stone are rejected
1：1：1 parts by weight Corn stalk, Chicken dung and peat soil prepares carrier；
（2）The preparation of bacteria suspension：Promoting bacteria is subjected to activation culture in the medium, is prepared into every milliliter of CFU 2 × 108—3
×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, ratio is（3-5）ml/（4-8）G,
It is placed 16-20 days at 18 DEG C -22 DEG C after mixing.
Preferably, step（2）Promoting bacteria described in the preparation of bacteria suspension is that living bacteria count ratio is 1：1：1：1 consolidates
Nitrogen bacterium, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium carry out activation culture in potato dextrose agar and are made.
Preferably, step（3）In to hang the ratio of bacterium solution and carrier be 2ml：3g places 18 at 20 DEG C after mixing
My god, once agitation is carried out when every 2 is small during this period makes it be come into full contact with air.
A kind of Organic carbon microbial manure, it is prepared using any of the above-described preparation method.
Beneficial effects of the present invention are as follows：
Certain density plastc ring is made in nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium in the present invention, by this bacteria suspension
With maize straw, Chicken dung and peat soil made of carrier carry out the reasonably combined compound strain-carrier that is made and combine microorganism
Fertilizer.The present invention is arranged in pairs or groups by rational bacterial strain and carrier, and microorganism fertilizer obtained has good adsorption capacity, is conducive to strain
Existence and Function, and with fixed nitrogen, Soluble phosphorus, potassium decomposing and the bacterial strain of Anti-bacterium effect, plant is increased by its vital movement
The supply of nutrient secretes plant hormone to promote the growth of crops also by microorganism during growth and breeding, with
And antibiotic is secreted to inhibit the growth of pathogen around root system.
Specific embodiment
Embodiment 1
A kind of Organic carbon microbial manure and preparation method thereof, includes the following steps：
（1）The preparation of carrier：It air-dries, takes after impurity larger in maize straw, Chicken dung and peat soil and stone are rejected
1：1：1 parts by weight Corn stalk, Chicken dung and peat soil prepares carrier；
（2）The preparation of bacteria suspension：Promoting bacteria is subjected to activation culture in the medium, is prepared into every milliliter of CFU 2 × 108—3
×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, ratio is（3-5）ml/（4-8）G,
It is placed 16-20 days at 18 DEG C -22 DEG C after mixing.
Embodiment 2
A kind of Organic carbon microbial manure and preparation method thereof, includes the following steps：
（1）The preparation of carrier：It air-dries, takes after impurity larger in maize straw, Chicken dung and peat soil and stone are rejected
1：1：1 parts by weight Corn stalk, Chicken dung and peat soil prepares carrier；
（2）The preparation of bacteria suspension：Living bacteria count ratio is 1：1：1：1 nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium is in horse
Activation culture is carried out in bell potato glucose agar medium, is prepared into every milliliter of CFU 2 × 108—3×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, the ratio of outstanding bacterium solution and carrier is
2ml：3g is placed 18 days at 20 DEG C after mixing, and once agitation is carried out when every 2 is small during this period makes it fully be connect with air
It touches, prevents from generating anaerobic environment due to internal temperature is excessively high, and then be unfavorable for target bacteria growth.
Comparative example 1
A kind of Organic carbon microbial manure and preparation method thereof, includes the following steps：
（1）The preparation of carrier：It is air-dried after impurity larger in Chicken dung and stone are rejected, prepares carrier；
（2）The preparation of bacteria suspension：Living bacteria count ratio is 1：1：1：1 nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium is in horse
Activation culture is carried out in bell potato glucose agar medium, is prepared into every milliliter of CFU 2 × 108—3×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, the ratio of outstanding bacterium solution and carrier is
2ml：3g is placed 18 days at 20 DEG C after mixing, and once agitation is carried out when every 2 is small during this period makes it fully be connect with air
It touches, prevents from generating anaerobic environment due to internal temperature is excessively high, and then be unfavorable for target bacteria growth.
Comparative example 2
A kind of Organic carbon microbial manure and preparation method thereof, includes the following steps：
（1）The preparation of carrier：It is air-dried after impurity larger in peat soil and stone are rejected, prepares carrier；
（2）The preparation of bacteria suspension：Living bacteria count ratio is 1：1：1：1 nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium is in horse
Activation culture is carried out in bell potato glucose agar medium, is prepared into every milliliter of CFU 2 × 108—3×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, the ratio of outstanding bacterium solution and carrier is
2ml：3g is placed 18 days at 20 DEG C after mixing,
Once agitation is carried out when every 2 is small during this period makes it be come into full contact with air, prevents from generating due to internal temperature is excessively high and detest
Oxygen environment, and then it is unfavorable for target bacteria growth.
Comparative example 3
A kind of Organic carbon microbial manure and preparation method thereof, includes the following steps：
（1）The preparation of carrier：It is air-dried after impurity larger in maize straw and stone are rejected, prepares carrier；
（2）The preparation of bacteria suspension：Living bacteria count ratio is 1：1：1：1 nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-rotten bacterium is in horse
Activation culture is carried out in bell potato glucose agar medium, is prepared into every milliliter of CFU 2 × 108—3×108Bacteria suspension.
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, the ratio of outstanding bacterium solution and carrier is
2ml：3g is placed 18 days at 20 DEG C after mixing, and once agitation is carried out when every 2 is small during this period makes it fully be connect with air
It touches, prevents from generating anaerobic environment due to internal temperature is excessively high, and then be unfavorable for target bacteria growth.
The measure of microbial manure technical indicator
1st, assay method
Living bacteria count is measured using plate counting method, and water content is measured using direct drying method；PH is measured using glass electrode method；
Organic matter is measured using potassium bichromate titrimetric method；Total nitrogen is measured using Micro-kjoldahl method；Total phosphorus is resisted using sour molten-molybdenum antimony
Colorimetric method for determining；Available potassium is measured using atomic absorption spectrophotometry.
2nd, result and analysis
（1）Living bacteria count
The living bacteria count for the microbial manure that embodiment 2, comparative example 1, the method for comparative example 2 and comparative example 3 are prepared is such as
Shown in table 1.
Table 1
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Living bacteria count/（Hundred million g-1） | 26.3 | 2.2 | 19.4 | 20.1 |
Living bacteria count is the characterization most important index of microorganism fertilizer quality, and as can be seen from Table 1, effective viable bacteria book accords with
It closes and due to standard NY/T798-2015《Composite microbiological fertilizer》The regulation of middle living bacteria count >=0.2 hundred million/g, in embodiment 2
The living bacteria count of microorganism fertilizer that is prepared of method it is most.
（2）PH value
PH value such as 2 institute of table for the microbial manure that embodiment 2, comparative example 1, the method for comparative example 2 and comparative example 3 are prepared
Show.
Table 2
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| PH value | 6.8 | 5.5 | 5.9 | 7.2 |
PH value is the important factor of microorganism stock, and pH value meets and due to standard NY/T798-2015《Composite microbe fertilizer
Material》In 5.5 ~ 8.5 regulation.
（3）The content of organic matter
The content of organic matter for the microbial manure that embodiment 2, comparative example 1, the method for comparative example 2 and comparative example 3 are prepared is such as
Shown in table 3.
Table 3
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| ω（Organic matter）% | 13.5 | 4.7 | 14.3 | 16.7 |
The content of organic matter is one of chemical parameters for reacting microorganism fertilizer quality-critical, in comparative example 3, comparative example 2 and embodiment 2
The content of organic matter of microorganism fertilizer that is prepared of method it is higher.
（4）Total nitrogen content
The total nitrogen content such as table 4 for the microbial manure that embodiment 2, comparative example 1, the method for comparative example 2 and comparative example 3 are prepared
It is shown.
Table 4
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| ω（Total nitrogen）% | 0.15 | 1.80 | 0.20 | 0.10 |
As can be known from Table 4, the total nitrogen content of microbial manure prepared by the method in comparative example 1 and comparative example 2 is higher.
（5）Total phosphorus content
The total phosphorus content such as table 5 for the microbial manure that embodiment 2, comparative example 1, the method for comparative example 2 and comparative example 3 are prepared
It is shown.
Table 5
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| ω（Total phosphorus）% | 1.7 | 1.9 | 1.3 | 1.0 |
As can be known from Table 5, the total phosphorus content for the microbial manure that the method in comparative example 1 and embodiment 2 is prepared is higher.
（6）Quick-acting potassium content
The microbial manure quick-acting potassium content such as table 6 that embodiment 2, comparative example 1, the method for embodiment 2 and comparative example 2 are prepared
It is shown.
Table 6
| Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| ω（Available potassium）（mg·g-1） | 0.14 | 0.17 | 0.15 | 0.09 |
As can be known from Table 6, the microbial manure available potassium that the method in comparative example 1, comparative example 2 and embodiment 2 is prepared contains
Amount is higher.
The microbial manure that method in comparative example 1 is prepared can provide abundant available potassium, but living bacteria count is non-
It is often low；The microbial manure that method in comparative example 2 is prepared cannot provide abundant living bacteria count and available nutrient；Than
There is higher living bacteria count and the content of organic matter, but nitrogen, phosphorus, potassium compared with the microbial manure that the method in example 3 is prepared
Constituent content is minimum；The microbial manure that method in embodiment 2 is prepared can not only provide abundant effective viable bacteria
Number, moreover it is possible to suitable nitrogen, phosphorus, potassium element are provided, are optimum strain-carrier combinations.
Claims (4)
1. a kind of preparation method of Organic carbon microbial manure, it is characterised in that：Include the following steps：
（1）The preparation of carrier：It air-dries, takes after impurity larger in maize straw, Chicken dung and peat soil and stone are rejected
1：1：1 parts by weight Corn stalk, Chicken dung and peat soil prepares carrier；
（2）The preparation of bacteria suspension：Promoting bacteria is subjected to activation culture in the medium, is prepared into every milliliter of CFU 2 × 108—3
×108Bacteria suspension；
（3）It will（2）In the outstanding bacterium solution for preparing be inoculated into（1）In the carrier of middle preparation, ratio is（3-5）ml/（4-8）G, mixing
It is placed 16-20 days at 18 DEG C -22 DEG C after uniformly.
2. a kind of preparation method of Organic carbon microbial manure according to claim 1, it is characterised in that：Step（2）Bacterium
Promoting bacteria described in the preparation of suspension is that living bacteria count ratio is 1：1：1：1 nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria and anti-corruption
Bacterium carries out activation culture in potato dextrose agar and is made.
3. a kind of preparation method of Organic carbon microbial manure according to claim 1, it is characterised in that：Step（3）In
Outstanding bacterium solution and the ratio of carrier are 2ml：3g is placed 18 days at 20 DEG C, carried out once when every 2 is small during this period after mixing
Agitation makes it be come into full contact with air.
4. a kind of Organic carbon microbial manure, it is characterised in that：It is obtained using such as any one of claim 1-3 preparation method preparation
.
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Application publication date: 20180529 |