CN101200385B - Preparation of composite microbial fertilizer by using bacilluslaterosporus and bacillus subtilis - Google Patents
Preparation of composite microbial fertilizer by using bacilluslaterosporus and bacillus subtilis Download PDFInfo
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- CN101200385B CN101200385B CN2006100224845A CN200610022484A CN101200385B CN 101200385 B CN101200385 B CN 101200385B CN 2006100224845 A CN2006100224845 A CN 2006100224845A CN 200610022484 A CN200610022484 A CN 200610022484A CN 101200385 B CN101200385 B CN 101200385B
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Abstract
The invention discloses a method for preparing compound microbial fertilizer by utilizing bacillus laterosporus and bacillus subtilis. The ingredients of the compound microbial fertilizer are: organic fertilizer of 50 percent to 70 percent, inorganic fertilizer of 20 percent to 40 percent and microbe of 2 percent to 8 percent. The microbe specifically comprises the bacillus laterosporus and the bacillus subtilis. The production process comprises that: 1. the bacillus laterosporus and the bacillus subtilis are planted in a PDA culture medium inclined plane to make the bacterium suspension, andthen are sprayed on the carrier so as to make spore powder. 2. the ingredients comprising livestock manure of 25 percent to 30 percent, kitchen garbage of 10 percent and 15 percent, soil carbon of 15percent to 20 percent, fungus chaff of 15 percent to 20 percent, straw powder of 10 percent to 15 percent, lemery salt of 5 percent to 10 percent, monocalcium phosphate and ordinary superphosphate of5 percent to 15 percent, calcium-magnesia phosphate fertilizer of 3 percent to 5 percent, boric sludge of 5 percent to 10 percent, green copperas of 0.5 percent to 1 percent, plaster of 1 percent to 2 percent and lime of 1 percent to 1.5 percent are mixed round equably, and the moisture is adjusted to 55 percent to 60 percent, and the organic fertilizer can be made after being fermented in a fermenter. 3. the bacillus laterosporus and bacillus subtilis spore powder of 2 percent to 8 percent are mixed round with the inorganic fertilizer of 20 percent to 40 percent and the organic fertilizer of50 percent to 70 percent equably, and then which are delivered into a drum granulator for granulation, after that, which are dried, cooled and screened to be packed.
Description
Technical field:
The present invention relates to a kind of composite microbiological fertilizer and preparation method thereof.
Background technology:
Whole world agriculture production just develops towards the direction of Organic farming, green agriculture, and this is a Development Trend of World Agriculture, also is the outlet of Agricultural Sustainable Development in China.Be engaged in organic grow, green agricultural product production, using microbial fertilizer is obligato basis.After the China's entry into the WTO, can food safety, food nutrition still march on towards the problem that the Chinese common people of well-off society pay close attention to the most comprehensively, are that can the agricultural-food of large agricultural country China go to the world yet, incorporate the key of world commerce mighty torrent.But, long-term a large amount of use of traditional chemical fertilizer in agriculture production, though make the output of agricultural-food be greatly improved, but employed chemical fertilizer has 70% loss approximately or is fixed in the soil, cause soil compaction, environmental pollution, infringement human health, because soil long-term lacking organic matter causes the crop products quality to descend, seriously restricting the Sustainable development of agricultural simultaneously.Addressing these problems the most basic way is usage quantity, raising chemical fertilizer utilization ratio, the increase soil organism that reduces chemical fertilizer.Therefore, composite microbiological fertilizer of the present invention is a kind of brand-new " competition inhibition, the eubiosis " concept product, have nontoxic, harmless, pollution-free, can make the plant ramp, have that fertilizer efficiency is long, heat-resisting, salt tolerant, anti-drying, acid and alkali-resistance, can prevention and elimination of disease and pests etc. characteristics, become the new-type fertilizer of agriculture production nuisance free crops.
Summary of the invention:
The object of the invention is to design a kind of composite microbiological fertilizer, and this fertilizer is that organic fertilizer, mineral manure, microbial bacteria are composited.
By design of the present invention, the composition of this composite microbiological fertilizer is: organic fertilizer 50-70%, mineral manure 20-40%, microbial bacteria 2-8%.
The present invention is realized by following three step technical schemes:
1, the preparation method of side gemma spore bacillus, Bacillus subtillis spore powder
1.1 primary inclined plane is cultivated: Side spore genus bacillus, subtilis are inoculated on the inclined-plane of PDA substratum 30 ℃ and cultivate after 24 hours and make bacteria suspension.
1.2 secondary seed is cultivated: in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion to 1% inoculum size, cultivates 48-72 hour for 25-30 ℃ with the bacterial suspension inoculation for preparing in proportion.
1.3 the preparation of spore powder: cultured seed liquid is sprayed on the carrier, and bake drying packages spare below 80 ℃.Detect gemma quantity, contain the gemma number at 20-70 hundred million/gram.
1.4 the side gemma spore bacillus, the Bacillus subtillis spore powder standard specimen that prepare are deposited in Microbiological Lab of Sichuan Aimengai Bio-technology Co., Ltd.
2, the preparation method of organic fertilizer
2.1 raw material is prepared:
2.1.1 animal dung: comprise that chicken and duck excrement, pig cow dung etc. dehydrate.
2.1.2 changing food waste: the solid-liquid separation dry packing is standby.
2.1.3 stalk: be ground into length and be 0.5-2 centimetre straw powder.
2.1.4 bacterium slag, peat: the bacterium slag with the spice machine break up drying, peat is standby with being ground into 40-60 order after drying.
2.1.5 mineral manure: vitriolate of tartar, mono-calcium phosphate and calcium superphosphate, fused(calcium magnesium)phosphate, boron mud, ferrous sulfate, lime, gypsum etc.
2.2 preparation of raw material: mix thoroughly by animal dung 25-30%, changing food waste 10-15%, peat 15-20%, bacterium slag 15-20%, straw powder 10-15%, vitriolate of tartar 5-10%, mono-calcium phosphate and calcium superphosphate 5-15%, fused(calcium magnesium)phosphate 3-5%, boron mud 5-10%, ferrous sulfate 0.5-1%, gypsum 1-2%, lime 1-1.5% batching, adjusting contains moisture in the 55-60% scope, the fermenter fermentation.
2.3 fermentation: will get above-mentioned respectively material ready and put into fermenter, and evenly add enzymatic microorganism simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen.One time fermentation requirement 12-15 days, the fermentation top temperature must reach 65-75 ℃ of degree.Secondary Fermentation needs 5-7 days micro-aerobe fermentations, and temperature is controlled at 48-52 ℃.
2.4 oven dry, pulverizing: the material that ferments is dried through fluidized-bed, and temperature is controlled at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches the 60-80 order, and is standby.
3, the preparation of composite microbiological fertilizer
3.1 (nitrogenous fertilizer can be used urea, ammonium sulfate with the mineral manure of Side spore genus bacillus, subtilis spore powder and the 20-40% of 2-8%, phosphate fertilizer phosphoric acid one ammonium, general calcium, potash fertilizer can be with vitriolate of tartar, Repone K) send into stirrer and make one-level and stir, the organic fertilizer that adds 50-70% is evenly again done the secondary stirring, makes material full and uniform.
3.2 the above-mentioned material that will stir is sent into the cylinder tablets press after crushed with travelling belt, uperize makes material be heated to 40-70 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up.
3.3 drying roller is sent in balling-up, input hot-air seasoning, temperature in≤360 ℃, temperature out≤50 ℃.
3.4 with the oven dry balling-up through cooling, screening, canned one-tenth finished product.
Claims (1)
1. composite microbiological fertilizer with the preparation of bacillus laterosporus, subtilis, it is characterized in that: the prescription of this composite microbiological fertilizer is made up of organic fertilizer, mineral manure and microbial bacteria, and its weight proportion is:
Organic fertilizer 50-70%, mineral manure 20-40%, microbial bacteria 2-8%;
Wherein organic fertilizer is by being that the monoammonium phosphate of bacterium slag 15-20%, straw powder 10-15% remaining behind animal dung 25-30%, changing food waste 10-15%, peat 15-20%, the edible fungus culturing of weight proportion, vitriolate of tartar 5-10%, 5-15% and calcium superphosphate mixture, fused(calcium magnesium)phosphate 3-5%, boron mud 5-10%, ferrous sulfate 0.5-1%, gypsum 1-2%, lime 1-1.5% mix the back and add enzymatic microorganism with raw material, make after fermentation, oven dry, pulverizing, its water content is 10-15%;
Wherein mineral manure is the mixture of nitrogenous fertilizer, phosphate fertilizer and potash fertilizer;
Wherein microbial bacteria is bacillus laterosporus spore powder, subtilis spore powder mixture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006100224845A CN101200385B (en) | 2006-12-13 | 2006-12-13 | Preparation of composite microbial fertilizer by using bacilluslaterosporus and bacillus subtilis |
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| CN2006100224845A CN101200385B (en) | 2006-12-13 | 2006-12-13 | Preparation of composite microbial fertilizer by using bacilluslaterosporus and bacillus subtilis |
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| CN101200385A CN101200385A (en) | 2008-06-18 |
| CN101200385B true CN101200385B (en) | 2011-01-12 |
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| CN101844944B (en) * | 2009-03-23 | 2013-04-24 | 北京世纪阿姆斯生物技术股份有限公司 | Total nutrient liquid microbial fertilizer |
| CN102010249B (en) * | 2010-10-29 | 2012-10-31 | 西安新天地草业有限公司 | Method for preparing composite biological organic fertilizer |
| CN102173924B (en) * | 2011-01-10 | 2013-03-27 | 艾应伟 | Preparation method of special biofertilizer for tobaccos |
| CN102153387B (en) * | 2011-05-13 | 2013-06-26 | 贵州省畜牧兽医研究所 | Method for preparing brown nitrogen-fixing bacteria microbial bacterial fertilizer |
| CN102153425A (en) * | 2011-05-17 | 2011-08-17 | 邢志胜 | Slow-releasing organic microbial urea and preparation method thereof |
| CN102424627A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special composite microbial fertilizer for vegetables and preparation method thereof |
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| CN102795931B (en) * | 2012-08-14 | 2013-10-16 | 王学文 | Biological organic fertilizer containing compound bacteria and preparation method |
| CN103360132A (en) * | 2013-07-25 | 2013-10-23 | 瑞昊（北京）环境工程有限公司 | Bio-organic fertilizer with kitchen garbage as raw material and preparation method thereof |
| CN103342615B (en) * | 2013-07-25 | 2016-01-20 | 瑞昊(北京)环境工程集团有限公司 | A kind of phosphorus decomposing, potassium decomposing biological organic fertilizer and preparation method thereof |
| CN103965916A (en) * | 2014-05-07 | 2014-08-06 | 苟俊 | Soil restoration agent, method for preparing soil restoration agent and method for restoring soil |
| CN104086306A (en) * | 2014-07-04 | 2014-10-08 | 四川新绿盛生物工程技术有限公司 | Special compound microbial fertilizer for kiwi fruits and preparation method thereof |
| CN104926478B (en) * | 2015-06-11 | 2017-09-19 | 湖北华丰生物化工有限公司 | A kind of slow controlled release compound organic fertilizer and preparation method thereof |
| CN105272406A (en) * | 2015-10-26 | 2016-01-27 | 北京鑫鑫盛达生物工程技术有限公司 | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
| CN105541423A (en) * | 2016-01-06 | 2016-05-04 | 长春孙老乐生物科技开发有限公司 | Microbial fertilizer containing bacillus subtilis |
| CN105837315A (en) * | 2016-03-24 | 2016-08-10 | 福建省农业科学院土壤肥料研究所 | Bio-bacterial fertilizer through composition of microbial agent and organic fertilizer and preparation method thereof |
| HU231292B1 (en) | 2016-11-22 | 2022-09-28 | Zoltán Sólyom | Method for preserving soil bacteria, preferably for preparing soil improving biomanure composition |
| CN109180232A (en) * | 2018-10-30 | 2019-01-11 | 王德桢 | Family's kitchen garbage microbe decomposer |
| CN110128193A (en) * | 2019-04-26 | 2019-08-16 | 茂县垃圾王环保科技有限公司 | A kind of Organic-inorganic composite microbial manure fertilizer technique |
| CN115073240A (en) * | 2022-07-05 | 2022-09-20 | 高唐亚泰肥料有限公司 | Fertilizer for improving agricultural soil and processing technology thereof |
| CN116253587B (en) * | 2023-02-08 | 2024-08-13 | 新疆维吾尔自治区葡萄瓜果研究所 | Microbial agent for increasing yield of grapes and preparation method thereof |
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| CN1348939A (en) * | 2000-10-18 | 2002-05-15 | 上海大洋生态有机肥有限公司 | Ecological organic fertilier and its prodn. process |
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| CN1762917A (en) * | 2005-09-01 | 2006-04-26 | 孙明坤 | Microorganism fertilizer and its preparation method |
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