CN1762917A - Microorganism fertilizer and its preparation method - Google Patents
Microorganism fertilizer and its preparation method Download PDFInfo
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- CN1762917A CN1762917A CN 200510097949 CN200510097949A CN1762917A CN 1762917 A CN1762917 A CN 1762917A CN 200510097949 CN200510097949 CN 200510097949 CN 200510097949 A CN200510097949 A CN 200510097949A CN 1762917 A CN1762917 A CN 1762917A
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- 238000002360 preparation method Methods 0.000 title claims description 9
- 239000003337 fertilizer Substances 0.000 title abstract description 21
- 244000005700 microbiome Species 0.000 title description 10
- 230000000813 microbial effect Effects 0.000 claims abstract description 32
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 17
- 235000000346 sugar Nutrition 0.000 claims abstract description 15
- 239000002689 soil Substances 0.000 claims abstract description 13
- 229910001868 water Inorganic materials 0.000 claims abstract description 13
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004202 carbamide Substances 0.000 claims abstract description 10
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 7
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 6
- 239000004467 fishmeal Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims description 30
- 238000012258 culturing Methods 0.000 claims description 26
- 230000001580 bacterial effect Effects 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 238000011534 incubation Methods 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 7
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000012262 fermentative production Methods 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- 238000010564 aerobic fermentation Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 abstract description 3
- 229940072033 potash Drugs 0.000 abstract description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 abstract description 3
- 235000015320 potassium carbonate Nutrition 0.000 abstract description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 abstract 2
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- 235000013877 carbamide Nutrition 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 239000000618 nitrogen fertilizer Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000002686 phosphate fertilizer Substances 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 235000010333 potassium nitrate Nutrition 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 8
- 230000000050 nutritive effect Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229940005654 nitrite ion Drugs 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 150000003016 phosphoric acids Chemical class 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000786137 Halogeton glomeratus Species 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The microbial organic fertilizer is prepared with brown sugar, fish meal, wheat bran, urea, KH2PO4, KNO3, and water in certain weight proportion, and through inoculating Saccharomyces cerevisiae and Bacillus subtilis and fermenting. It is one kind of multifunctional liquid organic fertilizer and may be applied to foliage or soil. It contains comprehensive nutrients and can raise the utilization of nitrogen fertilizer, phosphate fertilizer and potash fertilizer.
Description
Technical field
The present invention relates to a kind of microorganism organic fertilizer and preparation method thereof.
Background technology
Microbial fertilizer is a kind ofly to utilize the microbial life activity and make farm crop obtain a class fertilizer of specific fertilizer effect.In recent years, along with the continuous development of science and technology, produced both at home and abroad some fertilizer that utilize microbial fermentation to produce rapidly, wherein microorganism is based on vinelandii, phosphorus bacteria, potassium bacterium, a large amount of useful bacterial strain that screens from soil in addition.Present most microbial fertilizer utilizes existing nutritive ingredient to cultivate a large amount of microbial cells based on microbiobacterial agent, is adsorbed on certain carrier again.Utilizing a certain characteristic of microorganism to decompose to utilize what exist in the soil after using can not be a certain specific nutrition composition that crop directly utilizes.For example phosphorus bacteria can decompose the phosphoric acid salt in the soil, and the potassium bacterium can be decomposed the sylvite in the soil etc.The bacterial classification that these products use is single, acts on also single.
Summary of the invention
The technical issues that need to address of the present invention just be to overcome the bacterial classification that existing microbial fertilizer uses single, act on single defective, a kind of microbial fertilizer and preparation method thereof is provided, and microbial fertilizer of the present invention is the multi-functional microorganism organic fertilizer of a kind of liquid.
For addressing the above problem, the present invention adopts following technical scheme:
Microbial organic fertilizer of the present invention, it is the microbial organic fertilizer that is fermented and made by following weight proportion raw material access yeast saccharomyces cerevisiae (Saccharomgces cerevasae) and subtilis (Barillas subotilis),
Wherein the parts by weight of raw materials proportioning is: brown sugar 1-20 part, fish meal 1-15 part, wheat bran 0.1-5 part, urea 1-10 part, KH
2PO
40.1-6 part, KNO
30.1-4,100 parts in water.
The present invention provides a kind of method for preparing described microbial organic fertilizer simultaneously, and it comprises microbial strains cultivation and fermentative production step.
The culturing step of microbial strains described in the inventive method comprises yeast saccharomyces cerevisiae and subtilis at least twice enlarged culturing respectively.
Wherein, the yeast saccharomyces cerevisiae culturing step is:
1. enlarged culturing
Substratum weight part proportioning is: peptone 1%, glucose 1%, KH
2PO
40.3%, K
2HPO
40.5%, MgSO
47H
2O 0.2%, replenishes distilled water to 100%
By said ratio raw material is mixed, heating for dissolving is regulated pH value 5-6,121 ℃ of autoclavings 20 minutes,
Insert yeast saccharomyces cerevisiae bacterial classification constant temperature culture, temperature 28-30 ℃, incubation time 48h;
2. secondary enlarged culturing
Substratum weight part proportioning is: brown sugar 10%, urea 2-3%, KH
2PO
41%, make up water to 100%;
By said ratio raw material is mixed, heating for dissolving was boiled 20 minutes, regulated pH value 5-6, connect the spawn culture of an enlarged culturing, and temperature 28-30 ℃, incubation time 72h.
Wherein, the subtilis culturing step is:
1. enlarged culturing
Substratum weight part proportioning is: peptone 1%, and extractum carnis 0.3%, NaCl0.5% replenishes distilled water to 100%;
Take by weighing raw material by said ratio, the Hybrid Heating dissolving, transferring pH value is 7.0,121 ℃ of autoclavings 20 minutes, connect Bacillus subtilis strain and cultivate, temperature 33-35 ℃, incubation time 48h.
2. secondary enlarged culturing
Substratum weight part proportioning is: brown sugar 10%, dregs of beans 1%, make up water to 100%;
Take by weighing raw material by said ratio, heating for dissolving was boiled 20 minutes, was cooled to 33-35 ℃, inserted enlarged culturing spawn culture 72h one time.
The described fermentative production of the inventive method comprises the following steps:
(1) raw materials for production weight part prescription is: brown sugar 1-20 part, fish meal 1-15 part, wheat bran 0.1-5 part, urea 1-10 part, KH
2PO
40.1-6 part, KNO
30.1-4,100 parts in water;
(2) by said ratio raw material is mixed heated and boiled 20 minutes, cooling;
(3) yeast fermentation: controlled temperature 28-30 ℃, yeast saccharomyces cerevisiae is produced bacterial classification insert fermented liquid, fermentation 72h is divided into zero until residual sugar;
(4) fermentation of bacillus subtilis: after above-mentioned fermenting process is finished, be warming up to 33-35 ℃, add subtilis and produce bacterial classification, carry out aerobic fermentation, one week of time.
The inventive method can also comprise subsequent processing steps:
(1) filter: fermented liquid filters through 100 order gauzes.
(2) be in harmonious proportion: add micro-Fe Zn Cu Mo B Mn, mix.
Microbial organic fertilizer application process of the present invention can be foliage-spray or soil application.
A large amount of scientific experiments and production practice prove: the root of plant is the main path that absorbs nutritive substance.If cooperate the Foliage Absorption nutritive substance, effect can be better.Microbial organic fertilizer of the present invention is exactly a kind of microbial organic fertilizer that can soil adds leaf dressing, and the plant absorbing nutritive substance is abundant, and is effective.
Microbial organic fertilizer of the present invention is to utilize the farming, fishery byproduct and other composition that contain high protein to decompose to concentrate through microbial fermentation to form.In this process, produce a large amount of low-molecular-weight organic compounds that can directly be absorbed by crop, spray on plant leaf and can directly be absorbed the involved in plant Metabolic activity by blade, absorb inorganic fertilizer resynthesis organic matter than root system and reduced middle-chain and transport pathway, conserve energy, accelerate crop photosynthesis action intensity and photosynthetic rate, improve energy utilization ratio.
After microbial organic fertilizer of the present invention is manured into soil, microorganism in the fertilizer can be immersed plant root hair, makes plant roots generation transformation reactions, grows flourishing mycorhiza, help the nutriment in a fertilizer in the plant absorbing soil, and mycorhiza can directly synthesize a part of nutrition confession plant-growth needs.
The nutritive element that fertilizer of the present invention itself is contained is comprehensive, scientific formulation, and can improve the utilization ratio of nitrogen, phosphorus, potash fertilizer.
1, urea and ammonia easily are oxidized to nitrite ion by Nitrosomas in soil, emit NO simultaneously
2, N
2O, N
2Deng obnoxious flavour polluted source and air, cause nutriment in a fertilizer to run off simultaneously.Microorganism among the present invention can suppress the breeding of Nitrosomas in soil, and the enzyme of this microorganism secretion can continue nitrite ion and obnoxious flavour to be oxidized to nitric nitrogen or be reduced into ammonia-state nitrogen simultaneously, is made for thing and absorbs, and has so just improved the utilization ratio of nitrogen.
2, microorganism can produce a certain amount of acid in metabolic process, makes indissoluble materials such as calcium phosphate and phosphatic rock change into the phosphoric acid or the phosphoric acid salt of solubility, and crop can be absorbed.Microorganism can suppress the fixed action of available potassium in soil in addition, improves the utilization ratio of potash fertilizer.
Embodiment
Embodiment 1 yeast saccharomyces cerevisiae is cultivated
1. enlarged culturing
Substratum: peptone 10.0g, glucose 10.0g, KH
2PO
43.0g, K
2HPO
45.0g, MgSO
47H
2O 2.0g, make up water to 1 liter;
By said ratio raw material is mixed, heating for dissolving is regulated pH value 5-6, and 121 ℃ of autoclavings 20 minutes are cooled to 30 ℃, with the packing of 500ml triangular flask, every bottle of 200-250ml.
Insert the yeast saccharomyces cerevisiae bacterial classification, each triangle bottle graft 10-20ml bacterial classification, constant temperature culture, temperature 28-30 ℃, incubation time 48h;
2. secondary enlarged culturing
Substratum: brown sugar 1000g, urea 300g, KH
2PO
4100g, make up water to 10 liter;
By said ratio raw material is mixed, heating for dissolving was boiled 20 minutes, regulated pH value 5-6, connect 1 liter of cultivation of bacterial classification of an enlarged culturing, and temperature 28-30 ℃, incubation time 72h.
Embodiment 2 subtilises are cultivated
1. enlarged culturing
Substratum: peptone 10g, extractum carnis 3g, NaCl 5g replenishes distilled water to 1 liter;
Take by weighing raw material by said ratio, the Hybrid Heating dissolving, transferring pH value is 7.0,121 ℃ of autoclavings 20 minutes, is cooled to 35 ℃, with the packing of 500ml triangular flask, every bottle of 200-250ml.Connect Bacillus subtilis strain, each triangle bottle graft 10-20ml spawn culture, temperature 33-35 ℃, incubation time 48h.
2. secondary enlarged culturing
Substratum: brown sugar 1000g, dregs of beans 100g, make up water to 10 liter;
Take by weighing raw material by said ratio, heating for dissolving was boiled 20 minutes, was cooled to 33-35 ℃, inserted 1 liter of enlarged culturing bacterial classification, cultivated 72h.
Embodiment 3 fermentative production
(1) raw materials for production weight part prescription is: brown sugar 100kg, fish meal 85kg, wheat bran 15kg, urea 50kg, KH
2PO
425kg, KNO
310kg, water 1000kg;
(2) by said ratio raw material is mixed heated and boiled 20 minutes, cooling;
(3) yeast fermentation: controlled temperature 28-30 ℃, the yeast saccharomyces cerevisiae that embodiment 1 cultivates is produced 10% access that bacterial classification accounts for raw material weight by inoculum size, fermentation 72hr is divided into zero until residual sugar;
(4) fermentation of bacillus subtilis: after above-mentioned fermenting process is finished, be warming up to 33-35 ℃, the subtilis that embodiment 2 is prepared produces bacterial classification by 10% access that inoculum size accounts for raw material weight, carries out aerobic fermentation, in one week of time, finishes fermenting process.
Carry out subsequent disposal again:
(1) filter: fermented liquid filters through 100 order gauzes.
(2) be in harmonious proportion: add micro-Fe Zn Cu Mo B Mn, mix.
Embodiment 3 fertilisings
Foliage-spray: fertilizer dilution 300-400 doubly respectively sprays once in seedling phase, vegetative period, one-tenth strain phase, setting phase, sprays in the blade two sides, is moistening and does not drip and be advisable with plant.
Seed soaking or seed dressing: fertilizer dilution 100-200 doubly evenly dresses seed or seed soaking per hundred kilograms of seed fertilizer 100-120g.
Irritate root: 500 times of fertilizer dilutions, directly irritate and impose on crop root, mu consumption 5-10kg.
Claims (8)
1, a kind of microbial organic fertilizer, its characteristics are: it is the microbial organic fertilizer that is fermented and made by following weight proportion raw material access yeast saccharomyces cerevisiae (Saccharomgces cerevasae) and subtilis (Barillassubotilis),
Wherein the parts by weight of raw materials proportioning is: brown sugar 1-20 part, fish meal 1-15 part, wheat bran 0.1-5 part, urea 1-10 part, KH
2PO
40.1-6 part, KNO
30.1-4,100 parts in water.
2, a kind of method for preparing microbial organic fertilizer as claimed in claim 1, its characteristics are: it comprises microbial strains cultivation and fermentative production step.
3, the preparation method of microbial organic fertilizer as claimed in claim 2 is characterized in that: described microbial strains culturing step comprises yeast saccharomyces cerevisiae and subtilis at least twice enlarged culturing respectively.
4, the preparation method of microbial organic fertilizer as claimed in claim 3 is characterized in that: the yeast saccharomyces cerevisiae culturing step is:
1. enlarged culturing
Substratum weight part proportioning is: peptone 1%, glucose 1%, KH
2PO
40.3%, K
2HPO
40.5%, MgSO
47H
2O 0.2%, replenishes distilled water to 100%
By said ratio raw material is mixed, heating for dissolving is regulated pH value 5-6,121 ℃ of autoclavings 20 minutes,
Access accounts for the yeast saccharomyces cerevisiae bacterial classification of raw material weight 5-10%, constant temperature culture, temperature 28-30 ℃, incubation time 48h;
2. secondary enlarged culturing
Substratum weight part proportioning is: brown sugar 10%, urea 2-3%, KH
2PO
41%, make up water to 100%;
By said ratio raw material is mixed, heating for dissolving was boiled 20 minutes, regulated pH value 5-6, and the bacterial classification that inserts an enlarged culturing that accounts for raw material weight 5-10% carries out second incubation, and temperature 28-30 ℃, incubation time 72h.
5, the preparation method of microbial organic fertilizer as claimed in claim 4 is characterized in that: the subtilis culturing step is:
1. enlarged culturing
Substratum weight part proportioning is: peptone 1%, and extractum carnis 0.3%, NaCl 0.5%, replenishes distilled water to 100%;
Take by weighing raw material by said ratio, the Hybrid Heating dissolving, transferring pH value is 7.0,121 ℃ of autoclavings 20 minutes, connect the Bacillus subtilis strain that accounts for raw material weight 5-10% and cultivate, temperature 33-35 ℃, incubation time 48h.
2. secondary enlarged culturing
Substratum weight part proportioning is: brown sugar 10%, dregs of beans 1%, make up water to 100%;
Take by weighing raw material by said ratio, heating for dissolving was boiled 20 minutes, was cooled to 33-35 ℃, and insert an enlarged culturing bacterial classification that accounts for raw material weight 5-10% and carry out second incubation, temperature 28-30 ℃, time 72h.
6, the preparation method of microbial organic fertilizer as claimed in claim 5 is characterized in that: described fermentative production comprises the following steps:
(1) raw materials for production weight part prescription is: brown sugar 1-20 part, fish meal 1-15 part, wheat bran 0.1-5 part, urea 1-10 part, KH
2PO
40.1-6 part, KNO
30.1-4,100 parts in water;
(2) by said ratio raw material is mixed heated and boiled 20 minutes, cooling;
(3) yeast fermentation: controlled temperature 28-30 ℃, insert the yeast of raw material weight 5-10% and produce bacterial classification, fermentation 72hr is divided into zero until residual sugar;
(4) fermentation of bacillus subtilis: after above-mentioned fermenting process is finished, be warming up to the 33-35 ℃ of subtilis of inserting raw material weight 5-10% and produce bacterial classification, carry out aerobic fermentation, one week of time.
7, the preparation method of microbial organic fertilizer as claimed in claim 6 is characterized in that: it also comprises subsequent processing steps:
(1) filter: fermented liquid filters through 100 order gauzes;
(2) be in harmonious proportion: add micro-Fe Zn Cu Mo B Mn, mix.
8, the using method of microbial organic fertilizer according to claim 1, it is characterized in that: its application process is foliage-spray or soil application.
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|---|---|---|---|
| CN 200510097949 CN1289445C (en) | 2005-09-01 | 2005-09-01 | Microorganism fertilizer and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510097949 CN1289445C (en) | 2005-09-01 | 2005-09-01 | Microorganism fertilizer and its preparation method |
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|---|---|
| CN1762917A true CN1762917A (en) | 2006-04-26 |
| CN1289445C CN1289445C (en) | 2006-12-13 |
Family
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| CN101200385B (en) * | 2006-12-13 | 2011-01-12 | 四川艾蒙爱生物科技有限公司 | Preparation of composite microbial fertilizer by using bacilluslaterosporus and bacillus subtilis |
| CN101948754A (en) * | 2010-09-06 | 2011-01-19 | 成都和谐生物科技有限公司 | Microbial fermentation bed strain and manufacturing method thereof |
| CN101508601B (en) * | 2009-03-31 | 2011-05-04 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN102040405A (en) * | 2010-10-11 | 2011-05-04 | 山东郁茏生物科技有限公司 | Fish protein polypeptide biological bacterial fertilizer |
| CN103193541A (en) * | 2013-04-10 | 2013-07-10 | 陈松 | High-nitrogen-concentration composite microbial fertilizer and preparation method thereof |
| CN103214282A (en) * | 2013-04-28 | 2013-07-24 | 山东省农业科学院农业资源与环境研究所 | Microbial organic fertilizer and preparation method thereof |
| CN103508767A (en) * | 2013-09-22 | 2014-01-15 | 临汾市力澜生物科技有限公司 | Preparation method of organic biological fertilizer |
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