CN108031711A - A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading - Google Patents
A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading Download PDFInfo
- Publication number
- CN108031711A CN108031711A CN201810047625.1A CN201810047625A CN108031711A CN 108031711 A CN108031711 A CN 108031711A CN 201810047625 A CN201810047625 A CN 201810047625A CN 108031711 A CN108031711 A CN 108031711A
- Authority
- CN
- China
- Prior art keywords
- pesticide
- microbial fermenters
- microbial
- dry
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Fertilizers (AREA)
Abstract
A kind of application the present invention relates to microbial fermenters and its in Pesticide-Polluted Soil of degrading, by the present invention in that handling milk thistle shell with cellulase, and obtains a kind of microbial fermenters for the Pesticide-Polluted Soil that can effectively degrade by adding active dry yeast；The microbial fermenters that the present invention obtains effectively can not only degrade organophosphor in soil and pyrethrin pesticide, and can also substitute common nitrogen-phosphorus-potassium compound fertilizer, for promoting plant growth.
Description
Technical field
The invention belongs to microorganism and soil remediation field, and in particular to a kind of microbial fermenters and its degraded soil
Application in pesticide residue.
Background technology
Use of the pesticide in agricultural production, to eliminating pest and disease damage, roots out weeds, increases agricultural production and be made that and can not grind
The contribution gone out, but due to high poison, the use of high residue, difficult degradation pesticide, ecological environment and human health are also produced serious
Harm.According to statistics, the whole world uses more than 400 ten thousand tons of pesticides every year, wherein the pesticide more than 90% all remain in soil, air,
In water.People strengthen the continuous of environmental protection consciousness in recent years, in order to preserve the ecological environment and agricultural product security, there is an urgent need to
Exploitation is a kind of environmentally protective for removing Pesticide Residue in Soil bottom product.
The content of the invention
The present invention provides a kind of microbial fermenters, it is characterised in that the preparation method of the microbial fermenters is included such as
Lower step：
(1) dry milk thistle shell is taken, after being crushed, the cellulase that mass fraction is 1%-3% is soaked in and buffers
In solution, pH 4.5-5.0, soaking time for 5-6 it is small when after, filtering, is washed to neutrality, dry material；
(2) suitable dry ferment is uniformly mixed with the material that step (1) obtains, in 20-35oC, initial pH are 6.0-
Under conditions of 7.0, it is left to ferment 7-10 days, up to the microbial fermenters.
Crushed described in step (1) and be preferably crushed to 20-40 mesh；The preferred NaAc_HAc buffer solution of buffer solution, lemon
Lemon acid-sodium citrate buffer；The mass ratio of milk thistle shell and cellulase buffer solution is 1:5-10；Done in step (2)
The quality of yeast is the 3%-5% of milk thistle chitin amount.
Another embodiment of the present invention provides application of the mentioned microorganism fermentate in pesticide residue is removed.The agriculture
The preferred organophosphorus insecticide of medicine or pyrethrin pesticide, further preferred Hostathion, ethyl parathion, Fenpropathrin, adjacent benzene chrysanthemum ester
In one or more.
Another embodiment of the present invention provides application of the mentioned microorganism fermentate in soil remediation.
Another embodiment of the present invention provides mentioned microorganism fermentate and is preparing the soil remediation of removal pesticide residue
Application in agent.
Another embodiment of the present invention provides application of the mentioned microorganism fermentate as bio-organic fertilizer.
Another embodiment of the present invention provides the preparation method of mentioned microorganism fermentate, it is characterised in that including as follows
Step：
(1) dry milk thistle shell is taken, after being crushed, the cellulase that mass fraction is 1%-3% is soaked in and buffers
In solution, pH 4.5-5.0, soaking time for 5-6 it is small when after, filtering, is washed to neutrality, dry material；
(2) suitable dry ferment is uniformly mixed with the material that step (1) obtains, in 20-35oC, initial pH are 6.0-
Under conditions of 7.0, it is left to ferment 7-10 days, up to the microbial fermenters.
Crushed described in step (1) and be preferably crushed to 20-40 mesh；The preferred NaAc_HAc buffer solution of buffer solution, lemon
Lemon acid-sodium citrate buffer；The mass ratio of milk thistle shell and cellulase buffer solution is 1:5-10；Done in step (2)
The quality of yeast is the 3%-5% of milk thistle chitin amount.
Compared with prior art, it is the advantages of microbial fermenters of the present invention：(1) by the present invention in that using cellulase
Milk thistle shell is handled, and a kind of microbial fermentation for the Pesticide-Polluted Soil that can effectively degrade is obtained by adding active dry yeast
Thing；(2) microbial fermenters that the present invention obtains effectively can not only degrade organophosphor in soil and pyrethrin pesticide, and
And common nitrogen-phosphorus-potassium compound fertilizer can also be substituted, for promoting plant growth.
Embodiment
For the ease of a further understanding of the present invention, examples provided below has done more detailed description to it.But
It is that these embodiments only are not used for limiting the scope of the present invention or implementation principle, reality of the invention for being better understood from inventing
The mode of applying is not limited to herein below.
Embodiment 1
(1) dry milk thistle shell (1kg) is taken, is crushed to 20-40 mesh, is soaked in the fiber that mass fraction is 1%
In plain enzyme NaAc_HAc buffer solution (5kg), tune pH is 4.5-5.0, soaking time for 6 it is small when after, filtering, in being washed to
Property, dry material；
(2) dry ferment (30g) is uniformly mixed with the material that step (1) obtains, in 35 DEG C, initial pH be 6.0-7.0
Under the conditions of, it is left to ferment 7 days, up to the microbial fermenters (hereinafter referred to as product A).
Embodiment 2
(1) dry milk thistle shell (1kg) is taken, is crushed to 20-40 mesh, is soaked in the fiber that mass fraction is 3%
In plain enzyme NaAc_HAc buffer solution (10kg), tune pH is 4.5-5.0, soaking time for 5 it is small when after, filtering, in being washed to
Property, dry material；
(2) dry ferment (50g) is uniformly mixed with the material that step (1) obtains, in 20 DEG C, initial pH be 6.0-7.0
Under the conditions of, it is left to ferment 10 days, up to the microbial fermenters (hereinafter referred to as product B).
Embodiment 3
(1) dry milk thistle shell (1kg) is taken, 20-40 mesh is crushed to, obtains material；
(2) dry ferment (30g) is uniformly mixed with the material that step (1) obtains, in 35 DEG C, initial pH be 6.0-7.0
Under the conditions of, it is left to ferment 7 days, up to products C.
Embodiment 4
Dry milk thistle shell (1kg) is taken, is crushed to 20-40 mesh, is soaked in the cellulase that mass fraction is 1%
In NaAc_HAc buffer solution (5kg), tune pH is 4.5-5.0, soaking time for 6 it is small when after, filtering, is washed to neutrality, does
It is dry to obtain product D.
Embodiment 5
(1) dry milk thistle shell (1kg) is taken, is crushed to 20-40 mesh, is soaked in the fiber that mass fraction is 1%
In plain enzyme NaAc_HAc buffer solution (5kg), tune pH is 4.5-5.0, soaking time for 6 it is small when after, filtering, in being washed to
Property, dry material；
(2) dry ferment (10g) is uniformly mixed with the material that step (1) obtains, in 35 DEG C, initial pH be 6.0-7.0
Under the conditions of, it is left to ferment 20 days, up to product E.
Embodiment 6
The remaining Degrading experiment of Pesticide Residue in Soil
Pedotheque selects：
Sample 1：Hostathion, the content of ethyl parathion are 600mg/kg；
Sample 2：Fenpropathrin, the content of Biphenthrin are 600mg/kg.
Experimental method：7 parts of 20kg samples 1 and 2 are respectively taken, are separately added into product A-G (each 2g), are uniformly mixed, are ensured aqueous
After amount is when 20%-30%, 24 is small, then each add corresponding product A-G (each 2g) respectively, after mixing, 24 it is small when after,
The content of Pesticide Residue in Soil is detected, the results are shown in Table 1.
Table 1
Product F is the mixture of product D and 30g dry ferments that the embodiment 4 now made obtains
Product G is milk thistle shell powder (1kg), the mixture embodiment 7 of 300g cellulases and 30g dry ferments now made
Microbial fermenters of the present invention are alternatively arranged as biological organic fertilizer and are tried out in romaine lettuce plantation, 0.6 ton/hectare of fertilizer
(or 40kg/ mus), fertilizing type are applied, and usage time carries out when being strengthened for romaine lettuce.Fertilization depth 10cm, to dig after fertilising or
Blinding.When should be noted that during fertilising according to the adjustment fertilising of crop varieties, plant growth feature, soil fertility and weather meteorological condition
Between, dosage and method, and avoid fertilizer from directly directly being contacted with the root, stem, leaf of plant.
In order to verify the microbial organic fertilizer application effect of the present invention, field experiment has been carried out.Wherein, it is common multiple to apply
Hefei (N：P：K=15：15：15) as control (CK)；Product A, B and Common compound fertilizer (CK groups) dose are 40Kg/ mus,
Romaine lettuce kind is 4 season of Thailand.The result shows that compared with applying Common compound fertilizer (CK groups), the product of the present invention is applied in romaine lettuce
A, B, romaine lettuce blade area is larger, and plant height, width and blade quantity are higher, per mu yield increase 480-500kg/
Mu.
Claims (7)
1. a kind of microbial fermenters, it is characterised in that the preparation method of the microbial fermenters includes the following steps：
(1) dry milk thistle shell is taken, after being crushed, is soaked in the cellulase buffer solution that mass fraction is 1%-3%
In, pH 4.5-5.0, soaking time for 5-6 it is small when after, filtering, is washed to neutrality, dry material；
(2) suitable dry ferment is uniformly mixed with the material that step (1) obtains, in 20-35 DEG C, initial pH is 6.0-7.0's
Under the conditions of, it is left to ferment 7-10 days, up to the microbial fermenters.
2. the microbial fermenters described in claim 1, it is characterised in that the preferred acetic acid-vinegar of buffer solution described in step (1)
Sour sodium buffer solution, citric acid-sodium citrate buffer；The mass ratio of milk thistle shell and cellulase buffer solution is 1:5-
10；The quality of dry ferment is the 3%-5% of milk thistle chitin amount in step (2).
3. claim 1-2 any one of them microbial fermenters, it is characterised in that preferred crush is crushed described in step (1)
To 20-40 mesh.
4. application of the claim 1-3 any one of them microbial fermenters in pesticide residue is removed.
5. the application described in claim 4, it is characterised in that the preferred organophosphorus insecticide of the pesticide or pyrethrin pesticide.
6. the application described in claim 4-5, it is characterised in that the further preferred Hostathion of the pesticide, ethyl parathion, first
One or more in Cyano chrysanthemate, adjacent benzene chrysanthemum ester.
7. the preparation method of claim 1-3 any one of them microbial fermenters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810047625.1A CN108031711A (en) | 2018-01-17 | 2018-01-17 | A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810047625.1A CN108031711A (en) | 2018-01-17 | 2018-01-17 | A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108031711A true CN108031711A (en) | 2018-05-15 |
Family
ID=62096370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810047625.1A Pending CN108031711A (en) | 2018-01-17 | 2018-01-17 | A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108031711A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108239537A (en) * | 2018-01-17 | 2018-07-03 | 扬州工业职业技术学院 | A kind of preparation method for the soil-repairing agent for being used to remove pesticide residue |
| CN108728383A (en) * | 2018-06-09 | 2018-11-02 | 青岛农业大学 | A kind of preparation method of probiotics and its application in degrading pesticide residues |
| CN108727100A (en) * | 2018-06-09 | 2018-11-02 | 青岛农业大学 | A kind of probiotics and its application in preparing microbial organic fertilizer |
| CN109701199A (en) * | 2018-12-21 | 2019-05-03 | 四川惠泰农业科技有限公司 | A kind of biological agent that degrading plant agriculture is residual and its application |
| CN111574299A (en) * | 2020-05-28 | 2020-08-25 | 普乐格（厦门）生物科技有限公司 | Bacterial fertilizer for repairing plant leaf antioxidant system and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762917A (en) * | 2005-09-01 | 2006-04-26 | 孙明坤 | Microorganism fertilizer and its preparation method |
| CN107475164A (en) * | 2017-10-09 | 2017-12-15 | 李金奎 | A kind of composite bacteria agent and its pyrethroid pesticide remained purposes in soil of degrading |
| CN108239537A (en) * | 2018-01-17 | 2018-07-03 | 扬州工业职业技术学院 | A kind of preparation method for the soil-repairing agent for being used to remove pesticide residue |
-
2018
- 2018-01-17 CN CN201810047625.1A patent/CN108031711A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762917A (en) * | 2005-09-01 | 2006-04-26 | 孙明坤 | Microorganism fertilizer and its preparation method |
| CN107475164A (en) * | 2017-10-09 | 2017-12-15 | 李金奎 | A kind of composite bacteria agent and its pyrethroid pesticide remained purposes in soil of degrading |
| CN108239537A (en) * | 2018-01-17 | 2018-07-03 | 扬州工业职业技术学院 | A kind of preparation method for the soil-repairing agent for being used to remove pesticide residue |
Non-Patent Citations (1)
| Title |
|---|
| 马丽莉等: "酿酒酵母降解氯氰菊酯特性的研究", 《食品与发酵科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108239537A (en) * | 2018-01-17 | 2018-07-03 | 扬州工业职业技术学院 | A kind of preparation method for the soil-repairing agent for being used to remove pesticide residue |
| CN108728383A (en) * | 2018-06-09 | 2018-11-02 | 青岛农业大学 | A kind of preparation method of probiotics and its application in degrading pesticide residues |
| CN108727100A (en) * | 2018-06-09 | 2018-11-02 | 青岛农业大学 | A kind of probiotics and its application in preparing microbial organic fertilizer |
| CN109701199A (en) * | 2018-12-21 | 2019-05-03 | 四川惠泰农业科技有限公司 | A kind of biological agent that degrading plant agriculture is residual and its application |
| CN111574299A (en) * | 2020-05-28 | 2020-08-25 | 普乐格（厦门）生物科技有限公司 | Bacterial fertilizer for repairing plant leaf antioxidant system and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108031711A (en) | A kind of microbial fermenters and its application in Pesticide-Polluted Soil of degrading | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN104761379A (en) | Novel multifunctional compound microbial fertilizer and preparation method thereof | |
| CN108239537A (en) | A kind of preparation method for the soil-repairing agent for being used to remove pesticide residue | |
| CN101508602A (en) | Method for producing fertilizer | |
| CN110283601A (en) | A kind of acidic soil conditioner for improveing salt-soda soil | |
| CN108821875A (en) | A kind of preparation method of high-activity microorganism fertilizer | |
| CN106941958A (en) | A kind of method for culturing seedlings for hybridizing paper mulberry | |
| CN107155432A (en) | A kind of beta glucan content for improving crops and the method for improveing salt-soda soil | |
| CN105948960A (en) | Composite natural drug nicotine fertilizer and preparation method thereof | |
| CN112898076A (en) | Traditional Chinese medicine bacterial manure produced by utilizing traditional Chinese medicine waste residues and preparation method thereof | |
| CN105732136A (en) | Special bio-organic fertilizer for pepper and preparation method thereof | |
| CN107935639A (en) | The preparation method of efficient calcic organic fertilizer | |
| CN109054843B (en) | Soil conditioner, preparation method and application of soil conditioner as pesticide synergist | |
| CN105418206A (en) | Enzyme type dryland-raised rice seedling matrix and preparation method thereof | |
| CN106489694A (en) | A kind of Oryza sativa L. soilless breeding seeding Auricularia bacteria residue matrix substrate and preparation method | |
| CN110100519A (en) | A kind of fertilizing method for improving soil property, improving capsicum vitamin C and protein content | |
| CN102786352B (en) | Multifunctional organic liquid fertilizer and processing method thereof | |
| CN108456112A (en) | A kind of Special fertilizer for paddy rice | |
| CN105565915A (en) | Anti-grub soil conditioning biological fertilizer and production method thereof | |
| CN113698250B (en) | Sweet potato disease and pest biological control fertilizer and preparation method and application thereof | |
| CN110218122A (en) | Dedicated ecological organic fertilier of one planting fruit-trees and preparation method thereof | |
| CN104860783A (en) | Novel compound fertilizer capable of improving immunities of lettuces | |
| CN107915551A (en) | Plant sweet potato Chinese medicine slag biological fertilizer | |
| CN112655516A (en) | Solanaceous vegetable culture medium and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180515 |
|
| RJ01 | Rejection of invention patent application after publication |