CN103588544B - A kind of microbial organic fertilizer and its production and use - Google Patents
A kind of microbial organic fertilizer and its production and use Download PDFInfo
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 67
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 27
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- 239000002689 soil Substances 0.000 claims abstract description 7
- 229920000742 Cotton Polymers 0.000 claims description 19
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 241000196324 Embryophyta Species 0.000 claims description 9
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 7
- 240000007594 Oryza sativa Species 0.000 claims description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N D-Maltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 6
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- 238000011081 inoculation Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 claims description 4
- 244000053095 fungal pathogen Species 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
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- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
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- 229920001817 Agar Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
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- 241000233866 Fungi Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 241000227728 Trichoderma hamatum Species 0.000 description 1
- 241000378866 Trichoderma koningii Species 0.000 description 1
- 241000223262 Trichoderma longibrachiatum Species 0.000 description 1
- 241000123975 Trichoderma polysporum Species 0.000 description 1
- 241001304120 Trichoderma pseudokoningii Species 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 244000144972 livestock Species 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
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Abstract
The invention provides a kind of preparation method of microbial organic fertilizer, chlamydospore preparation process that the method comprises the steps: (1), in described chlamydospore preparation process, CGMCC by preserving number? the trichoderma harzianum strain of NO:5812 carries out liquid submerged fermentation to produce chlamydospore, (2) muck particle-making step, in described muck particle-making step, ight soil, humic acids and water are mixed, and inoculate deodorizing bacterial classification and carry out fermentative deodorizing, then obtain muck particle by granulation and drying; (3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.Present invention also offers the microbial organic fertilizer that preparation method as above obtains.Present invention also offers the purposes of microbial organic fertilizer as above in controlling plant diseases.By technique scheme, the present invention can improve the effect of Trichoderma controlling disease significantly.
Description
Technical field
The present invention relates to agricultural biological technical field, particularly, relate to a kind of microbial organic fertilizer, the preparation method of this microbial organic fertilizer and the purposes of this microbial organic fertilizer.
Background technology
Fertilizer is mainly made up of organism such as feces of livestock and poultry, crop material, humic acid, can provide desired nutritional, have and improve Soil structure, constantly the excellent good type fertilizer of the environment of Releasing nutrient for plant growth.Simultaneously fertilizer is also can provide the medium of good condition for the breeding of microorganism, is the important directions of current fertilizer science development.
Trichoderma (Trichodermaspp.) belongs to Deuteromycotina, hyphomycetes, Moniliales, sticky spore mushroom, be the ubiquitous saprophytic fungus of a class, be most potential biocontrol of plant disease bacterial strain in Mycophyta, can be used to prevent and treat various plants fungal disease.Common Trichoderma spp has trichoderma harziarum (T.harzianum), Kazakhstan wood mould (T.hamatum), many spores wood mould (T.polysporum), koning trichoderma (T.koningii), trichoderma pseudokiningii (T.pseudokoningii), viride (T.viride) and long shoot wood mould (T.longibrachiatum) etc.In numerous kinds, viride and trichoderma harziarum show particularly outstanding in Plant disease control prevention effect, are the bacterial strains producing upper main application at present.
Trichoderma can produce three kinds of propaguluies in its growth cycle, comprises mycelium, chlamydospore and conidium.The illumination of Trichoderma is on doleiform stigma, and conidium is subsphaeroidal, oval, or short obovate, obvious and small coarse projection on the smooth or cell walls of wall, light or colourless, size about 3.5 × 3.2 μm.Trichoderma usually forms chlamydospore, raw between on substrate mycelium, or it is raw at the tip end of vegetative hyphae side shoot, circular or oval, colourless to light yellow or green, size about 9.5 × 11.5 μm, smooth surface, cell walls has thickening phenomenon, and monogony produces, and usually has the ability of resistance to unsuitable environmental condition.Chlamydospore is the important propagulum form of Trichoderma.Trichoderma chlamydospore survival ability in soil is better than conidium, at least can survive 20 months.
Soil-borne disease is one of Major Diseases in the current agriculture production of China, and particularly on protecting field, for many years continuous cropping ground, soil-borne disease usually causes Severe Reduction, even ruins seedling total crop failure.Trichoderma can increase crop rhizosphere beneficial microorganism Microflora, improves the microbial bacteria group structure of crop rhizosphere, as the integral part of microbial organic fertilizer, can play the effect of controlling disease, but the effect of controlling disease still has much room for improvement.
Summary of the invention
The object of the invention is the effect improving Trichoderma controlling disease, a kind of microbial organic fertilizer with better controlling disease effect is provided.
To achieve these goals, the invention provides a kind of preparation method of microbial organic fertilizer, chlamydospore preparation process that the method comprises the steps: (1), in described chlamydospore preparation process, a kind of trichoderma strain is carried out liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is a bacterial strain of trichoderma harziarum (Trichodermaharzianum), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCCNO:5812; (2) muck particle-making step, in described muck particle-making step, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then obtain muck particle by granulation and drying; (3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.
Present invention also offers the microbial organic fertilizer that preparation method as above obtains.
Present invention also offers the purposes of microbial organic fertilizer as above in controlling plant diseases.
Pass through technique scheme, the present invention can improve the effect of Trichoderma controlling disease significantly, such as, relative to using the microbial organic fertilizer prepared for ACCC30371 trichoderma harziarum bacterial classification purchased from the trade names of Chinese agriculture microbial preservation administrative center, the disease index of cotton verticillium wilt can be reduced by more than 25% by microbial organic fertilizer of the present invention.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Biological deposits
The Classification And Nomenclature of bacterial strain of the present invention is trichoderma harziarum (Trichodermaharzianum), (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on February 27th, 2012, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution is abbreviated as CGMCC), deposit number is CGMCC:5812.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of preparation method of microbial organic fertilizer, chlamydospore preparation process that the method comprises the steps: (1), in described chlamydospore preparation process, a kind of trichoderma strain is carried out liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is a bacterial strain of trichoderma harziarum (Trichodermaharzianum), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCCNO:5812; (2) muck particle-making step, in described muck particle-making step, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then obtain muck particle by granulation and drying; (3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.
Wherein, the physiological status of described trichoderma strain is at least one in mycelium, chlamydospore and conidium.
Wherein, the operation of described liquid submerged fermentation can be carried out by liquid submerged fermentation method conveniently, under preferable case, the operation of described liquid submerged fermentation comprises the steps: (1) inoculation step, in described inoculation step, the conidium of trichoderma strain as above and/or mycelium are inoculated in liquid nutrient medium; (2) fermentation step, in described fermentation step, ferments the conidium and/or mycelium liquid substratum that are vaccinated with described trichoderma strain in fermentor tank.
Wherein, the conidium of described trichoderma strain and/or mycelium can use conventional slant culture to obtain, such as, the mycelium of trichoderma strain as above can be seeded in PDA substratum (containing potato 180-220g/L, glucose 18-22g/L, agar 10-25g/L) on, 5-7 days is cultivated at 27-29 DEG C, the conidium on substratum and/or mycelium can being attached in a large number, the substratum that slant culture terminates can being dug block to obtain conidium for inoculating and/or mycelium.Wherein, also the substratum that slant culture terminates can be dug block and be inoculated in enlarged culturing in liquid nutrient medium, obtain conidium and/or the mycelium of enlarged culturing, using the conidium of enlarged culturing and/or mycelium as the conidium being used for inoculating and/or mycelium.
Wherein, the condition of described fermentation can be conventional liquid submerged fermentation condition, and under preferable case, the condition of described fermentation comprises: the leavening temperature fermented 0-40 hour is 27.5-28.5 DEG C; The 40th hour later leavening temperature that ferments is 25.5-26.5 DEG C; The air flow fermented 0-10 hour is 1:0.55-0.65 volume: (volume minute); The air flow fermented 10-40 hour is 1:0.9-1.1 volume: (volume minute), and the 40th hour later air flow that ferments is 1:0.55-0.65 volume: (volume minute); Stirring velocity is 175-185rpm.
Wherein, described liquid nutrient medium can for the conventional substratum being applicable to the liquid submerged fermentation of Trichoderma, as substratum described in ZL200610057339.0, under preferable case, described liquid nutrient medium contains the material be mixed to get by following weight percent: the starch of 2-3.5%, the yeast powder of 1-2%, the corn steep liquor of 4-8%, the CaCO of 0.1-0.5%
3, 0.01-0.02% Zn
2+, 0.01-0.03% Mg
2+with the water of surplus.
Wherein, in order to improve chlamydosporic output further, the condition of described fermentation also comprises: at fermentation 60-63 hour, relative to the fermented liquid of every 100 liters, adds the starch of 0.45-0.55kg.
Wherein, what described fermentation was carried out can ferment the conventional time adopted, under preferable case for carrying out Trichoderma total time, fermentation is terminated at the 100-120 hours of fermentation, now, the mycelium of more than 90% all defines chlamydospore, thus can improve chlamydosporic output further.
Wherein, after terminating fermentation, can by filtering, the method such as centrifugal and dry is separated and obtains chlamydospore from the fermented liquid terminating fermentation, also can directly using the fermented liquid that terminates ferment as containing chlamydosporic product use.
Wherein, described muck particle can be the conventional various muck particles used, such as the condition of described muck particle-making step comprises: mixed by the water of the chicken manure of 200-400kg, the humic acid of 600-800kg and 200-600kg, and at 15-40 DEG C of bottom fermentation deodorizing 9-11 days after inoculating deodorizing bacterial classification 90-110g, the muck particle of the diameter 4-5mm then obtained after cylinder granulation and drying.Wherein, described deodorizing bacterial classification can be the conventional various bacterial classifications for deodorizing that use, that can be commercially available.Such as, can use purchased from the BM microbial inoculum-starter of Bao Rong bio tech ltd, Henan or the quick rotting agent purchased from Zhengzhou family estate biotechnology company limited.Wherein, the weight of deodorizing bacterial classification calculates with dry weight.
Wherein, the operation mixed with muck particle by described chlamydospore can for conventional married operation; namely mixed material can be used as microbial organic fertilizer and uses; in order to improve homogeneity and the storage property of mixed material; under preferable case; the operation of described mixing step can comprise: the chlamydospore of Trichoderma kind of the present invention of the muck particle of 900-1100kg, 0.8-1.2kg and the malt sugar of 1-3kg are carried out dressing at not higher than the temperature of 60 DEG C, obtains dressing product.The dressing product obtained has good homogeneity and storage property, can use as preferred microbial organic fertilizer.Wherein, under preferable case, the chlamydospore quantity in every gram of microbial organic fertilizer is no less than 10
5individual chlamydospore.
Present invention also offers the purposes of microbial organic fertilizer as above in controlling plant diseases.The Plant diseases that microbial organic fertilizer provided by the invention can be able to be prevented and treated other Trichoderma of routine all plays the effect of effectively control.Preferably, microbial organic fertilizer provided by the invention has better prevention effect to soilborne fungal pathogens; Particularly preferably, microbial organic fertilizer provided by the invention has better prevention effect to cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight.That is, preferred described soilborne fungal pathogens is at least one in cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight.
Wherein, the operation of controlling disease can conveniently utilize the operation of Trichoderma controlling disease to carry out, and such as, described microbial organic fertilizer directly can be applied to soil and carry out controlling disease.
Wherein, when described microbial organic fertilizer is applied to field, calculate with chlamydosporic gauge, amount of application can in very large range change, such as, can be 5 × 10
4to 2 × 10
5individual chlamydospore/square metre.
Below will be described the present invention by embodiment.
Embodiment 1
Get the potato 20g after peeling to boil and get juice in 20 minutes, juice and 2g glucose, 1.5g agar and water are made into 100ml, after bottling sterilizing, cooling obtains PDA substratum.
By the corn steep liquor of the yeast powder of the starch of 195kg, 130kg, 325kg, the CaCO of 19.5kg
3, 2.4kg(to be to make Zn
2+be 0.015 % by weight) ZnSO
4, 6.5kg MgSO
4(to make Mg
2+be 0.02 % by weight), add water to 6.5m
3, mixing also sterilizing, obtains liquid nutrient medium.
Be that CGMCCNO:5812 trichoderma harzianum strain is inoculated in PDA substratum by preserving number, cultivate 6 days at 28 DEG C; To inoculate and PDA substratum after cultivating digs block is inoculated in (the lawn 1cm of 100ml liquid nutrient medium inoculation PDA substratum growth in shaking flask liquid nutrient medium
2), under 28 DEG C of conditions with rotating speed 200rpm, cultivate 24 hours, as seed fermenter inoculum; By in the seed tank culture base after cultured shaking flask inoculum access sterilizing (inoculum size 2%(v/v)), under 28 DEG C of conditions with rotating speed 180rmp, cultivate 24 hours, become ferment-seeded; Ferment-seeded is accessed in fermentation tank culture medium and carry out control fermentation (inoculum size 10%(V/V)).Fermentation condition in fermentor tank is: the leavening temperature fermented 0-40 hour is 28 DEG C; The 40th hour later leavening temperature that ferments is 26 DEG C; The air flow fermented 0-10 hour is 1:0.6 volume: (volume minute); The air flow fermented 10-40 hour is 1:1 volume: (volume minute), and the 40th hour later air flow that ferments is 1:0.6 volume: (volume minute); Stirring velocity is 180rpm.
Feed supplement is carried out in the fermenting process of fermentor tank.Namely constantly little in fermentation the 62nd, relative to the fermented liquid of every 100 liters, add the starch of 0.5kg.
Ferment the 110th constantly little, more than 90% mycelia all forms chlamydospore, and fermented liquid is thinning, and fermented liquid press filtration now is by collecting the screening that press filtration obtains dry, obtains chlamydospore.This chlamydospore is carried out microscopic morphology observation, confirms that this chlamydospore has the chlamydospore form of typical trichoderma harziarum.
The water of the chicken manure of 300kg, the humic acid of 700kg and 330kg is mixed, and 15-40 DEG C of bottom fermentation deodorizing 10 days after inoculating deodorizing bacterial classification (the BM microbial inoculum-starter purchased from Bao Rong bio tech ltd, Henan) 100g, the muck particle of the diameter 4.5mm then obtained after cylinder granulation and drying.
The chlamydospore of trichoderma harziarum that the muck particle obtained by the present embodiment of 900-1100kg, the present embodiment of 0.8-1.2kg obtain and the malt sugar of 1-3kg carry out dressing at not higher than the temperature of 60 DEG C, obtain dressing product.
Comparative example 1
Adopting the method for embodiment 1 to prepare dressing product, is be the trichoderma harziarum bacterial classification of ACCC30371 purchased from the trade names of Chinese agriculture microbial preservation administrative center unlike, trichoderma harziarum bacterial classification used.
Testing example 1
Dressing product embodiment 1 and comparative example 1 obtained, as microbial organic fertilizer 1 and microbial organic fertilizer 2, is calculated with chlamydosporic gauge, and amount of application is 1 × 10
5individual chlamydospore/square metre; inorganic fertilizer (is executed urea 21kg for every mu; calcium superphosphate 52kg; potassium sulfate 11kg) as conventional fertilizer application; (Trichoderma is utilized to prevent and treat cotton verticillium wilt according to document; plant protection journal; 30th volume; 3rd phase in 2003; 284-288 page) in method; in 2010 in Shihezi Area of Xinjiang, compared for microbial organic fertilizer 1, microbial organic fertilizer 2 and the prevention effect of conventional fertilizer application on cotton verticillium wilt, the impact on output of cotton and affect cotton quality, result is as shown in table 1-3.Result proves, microbial organic fertilizer of the present invention can improve the effect of Trichoderma controlling disease significantly and improve the yield and quality of cotton.
Table 1 microbial organic fertilizer is to the preventive and therapeutic effect of cotton verticillium wilt
Table 2: microbial organic fertilizer is on the impact of output of cotton
Table 3: microbial organic fertilizer affects cotton quality, suede long (cm)
| Fertilising | On average (measure for 10 times) |
| Microbial organic fertilizer 1 | 33.95 |
| Microbial organic fertilizer 2 | 32.5 |
| Conventional fertilizer application | 29.5 |
Testing example 2
Dressing product embodiment 1 and comparative example 1 obtained, as microbial organic fertilizer 1 and microbial organic fertilizer 2, is calculated with chlamydosporic gauge, and amount of application is 5 × 10
4individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document (GB/T17980.92-2004, bactericidal agent for preventing and treating cotton yellow, blight) in method, compared for microbial organic fertilizer 1, microbial organic fertilizer 2 and conventional fertilizer application to the prevention effect of cotton wilt, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the disease index of cotton wilt on average have dropped 28%, microbial organic fertilizer 1 is compared with conventional fertilizer application, and the disease index of cotton wilt on average have dropped 52%.Result proves, microbial organic fertilizer of the present invention can improve the effect of Trichoderma controlling disease significantly.
Testing example 3
Dressing product embodiment 1 and comparative example 1 obtained, as microbial organic fertilizer 1 and microbial organic fertilizer 2, is calculated with chlamydosporic gauge, and amount of application is 2 × 10
5individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document, (trichoderma harziarum and derosal compound use are to the control of rice seedling damping-off, journal of Zhejiang university (agricultural and life science version), 35th volume, 65-70 page) in method, compared for microbial organic fertilizer 1, microbial organic fertilizer 2 and conventional fertilizer application are to the prevention effect of rice seedling blight, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the relative control effect of rice seedling blight on average improves 11%, microbial organic fertilizer 1 is compared with conventional fertilizer application, the relative control effect of rice seedling blight on average improves 40%.Result proves, microbial organic fertilizer of the present invention can improve the effect of Trichoderma controlling disease significantly.
Testing example 4
Dressing product embodiment 1 and comparative example 1 obtained, as microbial organic fertilizer 1 and microbial organic fertilizer 2, is calculated with chlamydosporic gauge, and amount of application is 4 × 10
5individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document, (biological pesticide " vegetables obtain health " is to the controlling experiment of banana blight, Jiangsu's agriculture science, 39th volume, 199-201 page) in method, compared for microbial organic fertilizer 1, microbial organic fertilizer 2 and conventional fertilizer application to the prevention effect of banana blight, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the relative control effect of banana blight on average improves 15%, microbial organic fertilizer 1 is compared with conventional fertilizer application, and the relative control effect of banana blight on average improves 50%.Result proves, microbial organic fertilizer of the present invention can improve the effect of Trichoderma controlling disease significantly.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
Claims (9)
1. a preparation method for microbial organic fertilizer, the method comprises the steps:
(1) chlamydospore preparation process, in described chlamydospore preparation process, a kind of trichoderma strain is carried out liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is the bacterial strain of trichoderma harziarum (Trichodermaharzianum), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCCNO:5812;
(2) muck particle-making step, in described muck particle-making step, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then obtain muck particle by granulation and drying;
(3) mixing step, in described mixing step, mixed with described chlamydospore by described muck particle, the operation of described mixing step comprises: the malt sugar of the muck particle of 900-1100kg, the described chlamydospore of 0.8-1.2kg and 1-3kg is carried out dressing at not higher than the temperature of 60 DEG C.
2. preparation method according to claim 1, wherein, the operation of described liquid submerged fermentation comprises the steps:
(1) inoculation step, in described inoculation step, is inoculated in liquid nutrient medium by the conidium of described trichoderma strain and/or mycelium;
(2) fermentation step, in described fermentation step, the conidium and/or mycelial liquid nutrient medium that are vaccinated with described trichoderma strain are fermented in fermentor tank, the condition of described fermentation comprises: the leavening temperature fermented 0-40 hour is 27.5-28.5 DEG C; The 40th hour later leavening temperature that ferments is 25.5-26.5 DEG C; The air flow fermented 0-10 hour is 1:0.55-0.65; The air flow fermented 10-40 hour is 1:0.9-1.1, and the 40th hour later air flow that ferment is 1:0.55-0.65; Stirring velocity is 175-185rpm, and described air flow is that fermentating liquid volume passes into the ratio of gas volume with per minute.
3. preparation method according to claim 2, wherein, described liquid nutrient medium contains the material be mixed to get by following weight percent: the starch of 2-3.5%, the yeast powder of 1-2%, the corn steep liquor of 4-8%, the CaCO of 0.1-0.5%
3, 0.01-0.02% Zn
2+, 0.01-0.03% Mg
2+with the water of surplus.
4. the preparation method according to Claims 2 or 3, wherein, the condition of described fermentation also comprises: at fermentation 60-63 hour, relative to the fermented liquid of every 100 liters, adds the starch of 0.45-0.55kg.
5. preparation method according to claim 1, wherein, the condition of described muck particle-making step comprises: mixed by the water of the chicken manure of 200-400kg, the humic acid of 600-800kg and 200-600kg, and at 15-40 DEG C of bottom fermentation deodorizing 9-11 days after inoculating deodorizing bacterial classification 90-110g, the muck particle of the diameter 4-5mm then obtained after cylinder granulation and drying.
6. according to the microbial organic fertilizer that the preparation method in claim 1-5 described in any one obtains.
7. the purposes of microbial organic fertilizer according to claim 6 in controlling plant diseases.
8. purposes according to claim 7, wherein, described Plant diseases is soilborne fungal pathogens.
9. purposes according to claim 8, wherein, described soilborne fungal pathogens is at least one in cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight.
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| CN103910545B (en) * | 2014-03-18 | 2016-05-18 | 福建省农业科学院农业生物资源研究所 | Utilize pig manure discarded object to produce the method for Trichoderma harzianum bacterial manure |
| CN103947563A (en) * | 2014-04-04 | 2014-07-30 | 上海德庄农业种植有限公司 | Stereo movable sheep collectively-breeding system |
| CN103980047B (en) * | 2014-05-19 | 2016-05-04 | 中国水稻研究所 | Microbial manure of a kind of water resistant rice sprouts damping-off and preparation method thereof |
| CN104230409B (en) * | 2014-09-16 | 2016-08-17 | 南京农业大学 | A kind of growth-promoting biological organic fertilizer and preparation method thereof |
| CN104446895B (en) * | 2014-11-10 | 2017-08-01 | 张常书 | Trichoderma as biological humic acid and its product |
| CN104761385A (en) * | 2015-04-11 | 2015-07-08 | 黑龙江八一农垦大学 | Multifunctional microbial organic fertilizer and preparation method thereof |
| CN105211106B (en) * | 2015-10-26 | 2018-04-24 | 广西南宁益土生物科技有限责任公司 | A kind of granule containing trichoderma harzianum and humic acid |
| CN108929166A (en) * | 2018-08-14 | 2018-12-04 | 上海绿乐生物科技有限公司 | A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism |
| CN108967095A (en) * | 2018-08-14 | 2018-12-11 | 文山市泽禄生物科技有限公司 | A kind of method that Radix Notoginseng is continuously cultivated |
| CN110078551B (en) * | 2019-05-10 | 2021-12-28 | 广西壮族自治区农业科学院 | Preparation method and application of disease-resistant microbial fertilizer |
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