CN102329739B - Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure - Google Patents
Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure Download PDFInfo
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- CN102329739B CN102329739B CN 201110254026 CN201110254026A CN102329739B CN 102329739 B CN102329739 B CN 102329739B CN 201110254026 CN201110254026 CN 201110254026 CN 201110254026 A CN201110254026 A CN 201110254026A CN 102329739 B CN102329739 B CN 102329739B
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- cake
- tung oil
- aspergillus versicolor
- oil tree
- little seeds
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Abstract
The invention relates to an Aspergillus versicolor for fermenting a Jatropha cake to manufacture biological bacterial manure, belonging to the field of manure. The Aspergillus versicolor provided by the invention is named as Aspergillus versicolor A003. The stain is preserved in a preservation unit designated by State Intellectual Property Office, the preservation date is 18th, July 2011, the name of the preservation unit is China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2011240. When the Aspergillus versicolor is inoculated on a culture medium mainly prepared from the Jatropha cake, the Jatropha cake can be quickly fermented, toxic components in the Jatropha cake can be eliminated, the detoxified Jatropha cake and live thalli growing on the Jatropha cake can be used as biological bacterial manure and can promote the growth of various crops.
Description
Affiliated technical field
The invention belongs to the bio-bacterial manure field, relate to a kind of can the little seeds of a tung oil tree grouts of fermentation, its toxic component and can promote the aspergillus versicolor of various plants growth of degrading.
Background technology
In short supply day by day along with petroleum resources greatly developed biomass energy and is the inevitable strategic choice that China improves energy security, ensures power supply.The little seeds of a tung oil tree (Jatropha curcas L. has another name called cream paulownia, Cortex jatrophae etc.) belong to Euphorbiaceae leprosy Pterostyrax; Be considered to develop the suitable seeds of biofuel; But its industrial development also is in commitment, has problems such as cost is higher, by product can't make full use of.
Little seeds of a tung oil tree grouts contain rich nutrient substances, can utilize nutritive ingredient in the little seeds of a tung oil tree cake as the substratum ferment making bio-bacterial manure of microorganism growth.Through making the bio-bacterial manure of high added value, can reduce little seeds of a tung oil tree production of biodiesel cost, thereby make it have price competitiveness preferably, help the industrialization of little seeds of a tung oil tree biofuel.
Along with improving constantly of living standards of the people, all come production safety, non-harmful green food both at home and abroad in the develop actively green agriculture.Chemical fertilizer, chemical pesticide and other chemical substance need not or be used in requirement as far as possible less in the production green food process.Microbial fertilizer not only can relax or reduce pollution of agricultural products, and can improve the quality of agricultural-food, and vast market prospect is arranged.
The mikrobe live body preparation that the ability various organic materialss of acceleration (comprising agricultural crop straw, feces of livestock and poultry, domestic refuse and municipal sludge etc.) decompose, become thoroughly decomposed is referred to as the organic material composting agent; It is the one type of bio-bacterial manure that has just grown up in recent years; Present organic material composting agent major part is that the quick composting with agricultural crop straw and feces of livestock and poultry is a target, does not see as yet that to be exclusively used in little seeds of a tung oil tree cake this not only toxic but also contain the organic material composting agent of higher protein content.
Utilize the patent of little seeds of a tung oil tree cake making fertilizer aspect application less.Zhao Qi etc. have applied for " a kind of jatropha mycorhiza bacterium composite fertilizer and preparation method thereof (200710195056.7) ".The invention discloses a kind of jatropha mycorhiza bacterium composite fertilizer, contain one or more arbuscular mycorrhiza class fungies (Arbuscular mycorrhizal fungi) of mould genus of capsule (Glomus) and the mould genus of huge sporangiocyst (Gigaspora).The problem that this method exists is: since capsule mould is a kind of plant symbiosis bacterium, can not leave live plant, must use live plant to cultivate, be unfavorable for this bacterium on a large scale, cultivation fast.Because this bacterium can not grow on artificial medium, little seeds of a tung oil tree grouts only play the effect of carrier, and unfermentable little seeds of a tung oil tree cake can't remove the toxicity of little seeds of a tung oil tree cake.
Contain toxic component in the little seeds of a tung oil tree cake, when being applied to the field less than the little seeds of a tung oil tree grouts as fertilizer sources that ferments, the toxic component that contains in the cake can dissolve in water body on every side, can cause very big pollution to environment, destroys the eubiosis, can influence people's health.
Owing to contain certain antimicrobial substance in the little seeds of a tung oil tree cake; Add that it contains high-load protein; A lot of mikrobes can not grow on little seeds of a tung oil tree cake fast; Can not degrade toxic component in the little seeds of a tung oil tree cake is not seen the relevant little seeds of a tung oil tree cake fermentation that can be used to ferment as yet and can be removed little seeds of a tung oil tree cake toxicity again, can also promote the report of the bacterial strain of plant-growth.Aspergillus versicolor is a common mikrobe in one type of environment, finds it after deliberation and can on the substratum that by little seeds of a tung oil tree cake is the staple preparation, grow fast, but do not see the report that aspergillus versicolor is used for bio-bacterial manure as yet.
Summary of the invention
The purpose of this invention is to provide a kind of have little seeds of a tung oil tree cake of quick fermentation and the little seeds of a tung oil tree grouts of ability effective elimination toxic composition, mikrobe---the former mutation A003 of the aspergillus versicolor bacterial strain (Aspergillus versicolor A003) of remarkable growth enhancing effect can also be arranged multiple kinds of crops.Being inoculated in this strain microorganism strains with little seeds of a tung oil tree cake is on the substratum of staple preparation, after cultivating, can removes the toxic component in the little seeds of a tung oil tree grouts.Through multiple living animal experiment, the grouts after fermentation, toxic component has been removed totally.Through the various plants cultivation experiments, the little seeds of a tung oil tree cake after the fermentation has tangible growth enhancing effect to various plants.
The former mutation A003 of aspergillus tubigensis called after aspergillus versicolor of the present invention Aspergillus versicolor A003, deposit number is CCTCC NO:M 2011249.
Aspergillus versicolor fungus strain of the present invention from the little seeds of a tung oil tree root portion of Yunnan Province of China Chuxiong from obtaining.Be deposited in the preservation of specified depositary institution of State Intellectual Property Office (depositary institution's title: Chinese typical culture collection center, preservation date are on July 11st, 2011, and deposit number is CCTCCNO:M2011249 at present.The depositary institution address: Wuhan Luo Jiashan, in the Wuhan University, postcode 430072).
Aspergillus versicolor of the present invention well-grown all on most of substratum.Because little seeds of a tung oil tree cake contains antimicrobial substance, a lot of mikrobes can not well grow on the substratum by little seeds of a tung oil tree cake preparation.Though have some bacterium on little seeds of a tung oil tree cake, to grow fast, can not remove the toxic component in the little seeds of a tung oil tree cake.Some mikrobes are also arranged, though can be on the substratum that is staple with the little seeds of a tung oil tree grouts well-grown, do not promote the effect of plant-growth.When the former mutation A003 of the aspergillus versicolor bacterial strain that obtains through several different methods, multiple index screening then can add or not add some little seeds of a tung oil tree cakes that can loosen, increases ventilation or adjust the material of nutritive ingredient proportioning in little seeds of a tung oil tree cake, can well-grown.Growth has the little seeds of a tung oil tree grouts culture of the former mutation A003 of aspergillus versicolor, and toxic component can be by this strain mikrobe effective elimination.Little seeds of a tung oil tree cake after detoxification and above the aspergillus versicolor of growth, can be used as fertilizer, promote plant-growth.
Description of drawings
Fig. 1. the colonial morphology of strains A 003 on the Cha Shi substratum
Fig.1Colonial?Morphological?characteristics?of?Aspergillus?versicolorA003on?Czapek’s?plate.
The spore head and the conidium of Fig. 2 strains A 003
Fig.2-11Spore?and?conidiophore?morphological?characteristics?of?Aspergillus?versicolor?A003?on?Czapek’s?plate.
Fig. 3. aspergillus versicolor A003 and the phylogenetic tree of part correlation bacterium in the Genbank DB.
Fig.3Phylogeny?tree?of?Aspergillus?versicolor?A003and?related?strains?in?Genbank?database.
Strain characteristics: the bacterium colony limitation of on Cha Shi agar, growing, 25 ℃ of 7 days diameter 16mm, 10-12 days is the velvet shape for the 24-30mm. quality.Bacterium colony is uneven, and is thick slightly, and the middle body protuberance is perk in various degree.Color is a white, or just be white, after be faint yellow, be secretly olive colour after again.Radial rill is arranged, and transudate does not have.The back side is khaki.Conidial head is radiation shape, diameter 25-125 μ m, and the conidium stalk is smooth, colourless.The product spore structure is double-deck, metulae 5-8 μ m * 2-3 μ m, bottle stalk 6-8 μ m * 1.5-2.5 μ m; Top capsule semisphere, diameter 9-20 μ m, about 3/4 surface can be educated.Conidium is spherical, diameter 2.0-3.0 μ m, tool spinule.
Culture condition: 28 ℃ of Cha Shi substratum
Slant culture condition: 28 ℃ of Cha Shi substratum
Seed culture condition: 28 ℃ of PDA liquid nutrient mediums
The little seeds of a tung oil tree cake of this strain fermentation is made the technical process of detoxification fertilizer:
The cane powder of the little seeds of a tung oil tree cake → a certain proportion of wheat bran of adding or the little seeds of a tung oil tree, water → sterilization → inoculation → cultivation → drying → finished product
The little seeds of a tung oil tree cake of detoxification of the present invention making method is made up of following steps:
One, cultivates the former mutation A003 of aspergillus versicolor with wheat bran substratum or liquid nutrient medium.
Two, cultured bacterial classification is sneaked on the little seeds of a tung oil tree cake solid medium after the sterilization.
Three, under 28 ℃ of conditions, cultivated 3-5 days.
Four, the results culture promptly can be used as fertilizer after drying.
Little seeds of a tung oil tree grouts of the present invention comprise the squeeze cake that obtains with milling process, also comprise the Jatropha curcas L dregs that obtains with the solvent extraction method.
The technical problem that the present invention will solve is the effect of utilizing the little seeds of a tung oil tree grouts of aspergillus versicolor ability quick fermentation, the little seeds of a tung oil tree cake toxic composition of degraded and promoting the multiple kinds of crops growth, solves the problem of utilizing that limits little seeds of a tung oil tree cake.
This technology is made up of following characteristic: preparation contains the solid or the liquid nutrient medium of little seeds of a tung oil tree cake; With medium sterilization, the former mutation A003 of inoculation aspergillus versicolor bacterial strain is cultivated; Cultured culture promptly can be used as bio-bacterial manure after cryodrying.
Beneficial effect is: aspergillus versicolor can be grown on little seeds of a tung oil tree cake fast, helps scale operation; Can specificity degrade toxic component in the little seeds of a tung oil tree cake after the little seeds of a tung oil tree are applied to the field, can not pollute environment; Can promote plant-growth, be applied to the field after, can improve the output of crop.
Specific embodiment
1, in little seeds of a tung oil tree cake: water was in 1: 1 ratio preparing culture medium; Press the ordinary method sterilization after 60 minutes; Cooling, the inoculation former mutation A003 of aspergillus versicolor (Aspergillus versicolor A003) bacterial strain is cultivated after 2 days; Aspergillus versicolor covers with substratum, and the little seeds of a tung oil tree cake that growth is good can obtain the little seeds of a tung oil tree cake bio-bacterial manure of detoxification after drying.
2, in little seeds of a tung oil tree cake: little seeds of a tung oil tree cane powder: 7: 3: 10 ratio obtaining liq substratum of water; The substratum 50 of in the 500ml triangular flask, packing into restrains; 1 normal atmosphere sterilization is after 1 hour, and aspergillus versicolor A003 is inoculated in cooling; Cultivate a week for 28 ℃, cryodrying promptly gets the bio-bacterial manure of the little seeds of a tung oil tree cake of detoxification.
Claims (1)
1. a strain aspergillus versicolor; It is characterized in that the former mutation Aspergillus of called after aspergillus versicolor versicolor A003; Specified depositary institution of State Intellectual Property Office preservation now; Preservation date is on July 11st, 2011, and depositary institution's name is called Chinese typical culture collection center, and deposit number is CCTCC NO:M2011249.
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| CN 201110254026 CN102329739B (en) | 2011-08-31 | 2011-08-31 | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure |
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| CN102424799A (en) * | 2011-12-27 | 2012-04-25 | 云南大学 | Aspergillus versicolor strain for manufacturing detoxicated feedstuff through fermentation of Jatropha curcas cakes |
| CN102835543B (en) * | 2012-09-27 | 2014-02-12 | 昆明理工大学 | Production method for jatropha curcas oil cake detoxification vegetable protein of biodiesel by-product |
| CN104004662A (en) * | 2014-05-22 | 2014-08-27 | 黑龙江大学 | Aspergillus versicolor strain with antibacterial activity |
| CN104082525B (en) * | 2014-06-19 | 2016-05-04 | 四川大学 | The application of strange land Klebsiella in barbadosnut pulp reduce toxicity of dwelling |
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| CN101240246A (en) * | 2007-02-05 | 2008-08-13 | 南京林业大学 | Rhizipogen luteous agent breeding optimal plan |
| CN101113411B (en) * | 2007-06-27 | 2010-11-17 | 东华大学 | Induction preparation of EGCG esterase and method for producing EGC and gallic acid by using the enzyme |
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