CN101240246A - Rhizipogen luteous agent breeding optimal plan - Google Patents
Rhizipogen luteous agent breeding optimal plan Download PDFInfo
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- CN101240246A CN101240246A CNA2007100199547A CN200710019954A CN101240246A CN 101240246 A CN101240246 A CN 101240246A CN A2007100199547 A CNA2007100199547 A CN A2007100199547A CN 200710019954 A CN200710019954 A CN 200710019954A CN 101240246 A CN101240246 A CN 101240246A
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Abstract
The invention discloses a cultured propagated scheme of optimum liquid of pinaster superior ectomycorrhiza fungal Rhizipogen luteous,HXW and solid bacterium agent. 1. the optimum fluid nutrient medium compositions is 200g white potato, 10g amylaceum, 40g wheat bran, 1000ml water, the optimum cultured conditional parameter is pH value is 5, temperature is 23 DEG C, oxygen-dissolving quantity is 100ml wigwagg bottle, the filling bottle quantity is three five, the revolution rate of swiging bed is 121r/min. 2. the optimum solid culture medium compositions is the rate of wheat bran: maize flour: turf: vermiculite is 1:1:1:7 and 25144403240ptimum fluid nutrient medium. The cultured conditional parameter is Ph is 5, temperature is 23 DEG C, the rate of solid substrates: liquid ground mass is 4:1. The organism growth quantity of the Rhizipogen luteous in optimum fluid nutrient medium is 80 16592gher than the organism growth quantity in usual PD nutrient medium after the Rhizipogen luteous is cultured in optimum fluid nutrient medium for 15d. The organism growth quantity is improved by 60after the Rhizipogen luteous is cultured in optimum fluid nutrient medium for 20d. the invention relates to forestry production, bacterial manure production etc. domain. The scheme is suitable for building and cultivating enlarging and producing of bacterial manure needed in commerical forest,prevention forest and urban ecology forest.
Description
Technical field
The present invention relates to fields such as production of forestry, bacterial manure production.Specifically, provide a kind of pine tree had the good ectomycorrhiza fungi of growth-promoting functions and degeneration-resistant the effect yellow optimal liquid and the multiplication technique of solid fungicide that must false truffle (Rhizipogen luteous), be suitable for building and cultivate the expansion of required bacterial manure in Timber stands, protection forest and the urban ecology woods numerous with production.
The technical background mycorhiza is that the root system of mycorrhizal fungi mycelia in the soil and higher plant forms the symbiosis association with specific modality 26S Proteasome Structure and Function.In plant materials, obtain necessary carbohydrate and other nutritive substances with the fungi of plant symbiosis, and plant obtains materials such as required nutrition and nutrient there from fungi, embody mutual benefit with this to help each other the high unity of exchanging each other's needs.The feature that the existing general root system of plant of mycorhiza is possessed has the characteristic that fungi had with plant symbiosis again.A lot of seeds lack mycorhiza just can not survive or grow poor.Remove and improve surviving rate, promote outside the plant-growth, mycorhiza can also improve abilities such as plant drought, anti-saline and alkaline, anti-extreme temperature and preventing from heavy metal murder by poisoning, strengthens the resistibility of plant to poor environment.In addition, mycorhiza also helps the control of plant to root disease.Therefore, mycorhiza plays an important role in Forest ecosystems.
Aspect mycorhiza research, compare with North America and some countries of Europe, China starts late, supporting technology to main reproducting tree species mycorrhizal seedling raising afforestation lacks systematic research, especially the mature technology that many mycorrhizal fungis still do not have separation, cultivate and breed, commercialization is produced and only is confined to certain several bacterial classification, the reliable method that also lacks the microbial inoculum development in addition and produce.Therefore, press on the growing seedlings, afforest, cultivate of pine tree good ectotrophic mycorrhiza is carried out the research of microbial inoculum multiplication technique, its application prospect is extensive.
Summary of the invention
The technical issues that need to address of the present invention are to filter out the good ectomycorrhiza fungi of pine tree---(Rhizipogenluteous, optimal liquid HXW) and solid fungicide cultivate and expand numerous scheme yellow palpus false truffle.
1, yellow must false truffle (Rhizipogen luteous, HXW) the optimal liquid culture medium prescription produced of microbial inoculum:
Potato 200g glucose 10g wheat bran 40g water 1000ml
Its optimal culture condition parameter: the pH value is 5, and temperature is 23 ℃, and dissolved oxygen amount is: 100ml shakes bottle, and the bottling amount is 3/5ths, and shaking speed is 121r/min.
2, yellow must false truffle (Rhizipogen luteous, HXW) the best solid culture substrate formula produced of microbial inoculum:
Wheat bran: Semen Maydis powder: the peat composed of rotten mosses: vermiculite=1: 1: 1: 7, add 25% optimal liquid culture medium again.
Its optimal culture condition parameter: the pH value is 5, and temperature is 23 ℃, and the solid-liquid proportioning is a solid substrate: fluid matrix=4: 1.
Cultivate the numerous scheme of expansion with existing ectomycorrhiza fungal inoculant and compare, progress of the present invention is:
(1) can obviously improve yellow palpus false truffle (Rhizipogen luteous, HXW) the biological growth amount in the liquid medium within.
(2) can obviously improve yellow palpus false truffle (Rhizipogen luteous, HXW) the biological growth amount in solid culture matrix.
Embodiment
Liquid culture expand numerous embodiment according to yellow must false truffle (Rhizipogen luteous, optimal liquid culture medium prescription HXW) are bottled after preparing corresponding substratum, and 0.5h sterilizes under 121 ℃, 1.01 * 106Pa condition.Under aseptic condition, with yellow must false truffle (Rhizipogenluteous HXW) moves and grows in the substratum.Put in the shaking table that rotating speed is 121r/min shaking bottle, cultivate 15d 23 ℃ of unglazed photographs under the condition.
Solid culture expand numerous embodiment according to yellow must false truffle (Rhizipogen luteous, best solid culture substrate formula HXW) pack after preparing corresponding substratum, and 2h sterilizes under 121 ℃, 1.01 * 106Pa condition.Under aseptic condition, (Rhizipogenluteous, liquid bacterial agent HXW) is poured in the culture bag with yellow palpus false truffle.Unglazed at 23 ℃ according to cultivating 20 days under the condition.
1 yellow must the biological growth amount of false truffle in optimal liquid substratum and PD substratum commonly used comparison of embodiment
Cultivate in the prescription at more than 20 kinds of mycorhiza bacteria liquids, just sift out 4 kinds of good formulas preferably, carry out multiple sieve on this basis again, thereby filter out the optimal liquid substratum.The result shows that yellow palpus false truffle is cultivated 15d in the optimal liquid substratum after, its biological growth amount is all higher than other, compares with the biological growth amount in the PD substratum commonly used, even exceeds 80%.
2 yellow must the biological growth amount of false truffle in best solid culture matrix and other culture mediumes comparisons of embodiment
Yellow palpus false truffle is cultivated 20d in best solid medium after, its biological growth amount is higher by 60% than the average biological growth amount in other culture mediumes.
Claims (2)
1. one kind is used for good ectomycorrhiza fungi---yellow palpus false truffle (Rhizipogen luteous, HXW) the optimal liquid culture medium prescription of microbial inoculum production: potato 200g glucose 10g wheat bran 40g water 1000ml
Its optimal culture condition parameter is: the pH value is 5, and temperature is 23 ℃, and dissolved oxygen amount is: 100ml shakes bottle, and the bottling amount is 3/5ths, and shaking speed is 121r/min.
2. one kind is used for good ectomycorrhiza fungi---yellow palpus false truffle (Rhizipogen luteous, HXW) the best solid culture substrate formula produced of microbial inoculum: wheat bran: Semen Maydis powder: the peat composed of rotten mosses: vermiculite=1: 1: 1: 7, add 25% optimal liquid culture medium again.
Its optimal culture condition parameter is: the pH value is 5, and 23 ℃ of temperature are solid substrate with the liquid proportioning: fluid matrix=4: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100199547A CN101240246A (en) | 2007-02-05 | 2007-02-05 | Rhizipogen luteous agent breeding optimal plan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100199547A CN101240246A (en) | 2007-02-05 | 2007-02-05 | Rhizipogen luteous agent breeding optimal plan |
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| CN101240246A true CN101240246A (en) | 2008-08-13 |
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| CNA2007100199547A Pending CN101240246A (en) | 2007-02-05 | 2007-02-05 | Rhizipogen luteous agent breeding optimal plan |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329739A (en) * | 2011-08-31 | 2012-01-25 | 云南大学 | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure |
| CN108624512A (en) * | 2018-05-30 | 2018-10-09 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation matrix, preparation method and the method for cultivating mycorhiza biological agent |
-
2007
- 2007-02-05 CN CNA2007100199547A patent/CN101240246A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329739A (en) * | 2011-08-31 | 2012-01-25 | 云南大学 | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure |
| CN108624512A (en) * | 2018-05-30 | 2018-10-09 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation matrix, preparation method and the method for cultivating mycorhiza biological agent |
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Open date: 20080813 |