CN103588544A - Microbial organic fertilizer, and preparation method and purpose thereof - Google Patents

Microbial organic fertilizer, and preparation method and purpose thereof Download PDF

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CN103588544A
CN103588544A CN201210286744.5A CN201210286744A CN103588544A CN 103588544 A CN103588544 A CN 103588544A CN 201210286744 A CN201210286744 A CN 201210286744A CN 103588544 A CN103588544 A CN 103588544A
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organic fertilizer
fermentation
microbial organic
trichoderma
chlamydospore
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CN103588544B (en
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陈舒
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Zhuhai Hengqin coordination Cci Capital Ltd
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BEIJING ZHONGNONG GUANGMAO AGRICULTURAL TECHNOLOGY Co Ltd
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Abstract

The invention provides a preparation method of a microbial organic fertilizer. The method comprises the following steps: (1) chlamydospore preparation by carrying out submerged fermentation on Trichoderma harzianum strains with preservation number of CGMCC NO:5812 to produce chlamydospores; (2) manure particle preparation by mixing manure, humic acid and water, and inoculating deodorizing bacteria for fermentation deodorization and granulating and drying to obtain the manure particles; and (3) mixing the manure particles and the chlamydospores. The invention also provides a microbial organic fertilizer obtained from the above preparation method. The invention also provides purpose of the microbial organic fertilizer in control of plant diseases. Through the above technical scheme, the invention can significantly improve the disease control effect of Trichoderma spp.

Description

A kind of microbial organic fertilizer and its production and use
Technical field
The present invention relates to agricultural biological technical field, particularly, relate to the preparation method of a kind of microbial organic fertilizer, this microbial organic fertilizer and the purposes of this microbial organic fertilizer.
Background technology
Fertilizer is mainly comprised of organism such as feces of livestock and poultry, crop material, humic acid, can provide desired nutritional for plant growth, has the Soil structure of improvement, discharges constantly the excellent good type fertilizer of environment of nutrient.Fertilizer is also for the breeding of microorganism, to provide the medium of good condition simultaneously, is the important directions of current fertilizer science development.
Trichoderma (Trichoderma spp.) belongs to Deuteromycotina, hyphomycetes, Moniliales, sticky spore mushroom, being the ubiquitous saprophytic fungus of a class, is most potential biocontrol of plant disease bacterial strain in Mycophyta, can be used to prevent and treat various plants fungal disease.Common Trichoderma kind has trichoderma harziarum (T.harzianum), Ha Shi wood mould (T.hamatum), many spores wood mould (T.polysporum), koning trichoderma (T.koningii), trichoderma pseudokiningii (T.pseudokoningii), viride (T.viride) and long shoot wood mould (T.longibrachiatum) etc.In numerous kinds, to prevent and treat aspect prevention effect performance particularly outstanding planting disease for viride and trichoderma harziarum, is to produce at present the bacterial strain of upper main application.
Trichoderma can produce three kinds of propaguluies in its growth cycle, comprises mycelium, chlamydospore and conidium.The illumination of Trichoderma is on doleiform stigma, and conidium is subsphaeroidal, ellipse, or short obovate, and obvious and small coarse projection on the smooth or cell walls of wall, light or colourless, size approximately 3.5 * 3.2 μ m.Trichoderma usually forms chlamydospore, raw between on substrate mycelium, or raw at the tip of vegetative hyphae side shoot end, circular or oval, colourless to light yellow or green, size approximately 9.5 * 11.5 μ m, smooth surface, cell walls has thickening phenomenon, and monogony produces, and conventionally has the ability of resistance to unsuitable environmental condition.Chlamydospore is the important propagulum form of Trichoderma.Trichoderma chlamydospore survival ability in soil is better than conidium, at least can survive 20 months.
Soil-borne disease is one of Major Diseases in the current agriculture production of China, is particularly protecting ground, the ground of continuous cropping for many years, and soil-borne disease usually causes Severe Reduction, even ruins seedling total crop failure.Trichoderma can increase crop rhizosphere beneficial microorganism Microflora, improves the microbial bacteria group structure of crop rhizosphere, as the integral part of microbial organic fertilizer, can play the effect that prevents and treats disease, and the effect of still preventing and treating disease still has much room for improvement.
Summary of the invention
The object of the invention is to improve the effect that Trichoderma prevents and treats disease, a kind of microbial organic fertilizer of better preventing and treating disease effect that has is provided.
To achieve these goals, the invention provides a kind of preparation method of microbial organic fertilizer, the method comprises the steps: (1) chlamydospore preparation process, in described chlamydospore preparation process, a kind of trichoderma strain is carried out to liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is a bacterial strain of trichoderma harziarum (Trichoderma harzianum), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO:5812; (2) muck particle preparation process, in described muck particle preparation process, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then by granulation and the dry muck particle that obtains; (3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.
The microbial organic fertilizer that the present invention also provides preparation method as above to obtain.
The present invention also provides the purposes of microbial organic fertilizer as above in controlling plant diseases.
Pass through technique scheme, the present invention can improve the effect that Trichoderma prevents and treats disease significantly, for example, with respect to using the microbial organic fertilizer of preparing for ACCC30371 trichoderma harziarum bacterial classification purchased from the trade names of Chinese agriculture microbial preservation administrative center, microbial organic fertilizer of the present invention can reduce more than 25% by the disease index of cotton verticillium wilt.
Other features and advantages of the present invention partly in detail are described the embodiment subsequently.
Biological preservation
The Classification And Nomenclature of bacterial strain of the present invention is trichoderma harziarum (Trichoderma harzianum), on February 27th, 2012, be deposited in (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, China Committee for Culture Collection of Microorganisms's common micro-organisms center, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution be abbreviated as CGMCC), deposit number is CGMCC:5812.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
The invention provides a kind of preparation method of microbial organic fertilizer, the method comprises the steps: (1) chlamydospore preparation process, in described chlamydospore preparation process, a kind of trichoderma strain is carried out to liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is a bacterial strain of trichoderma harziarum (Trichoderma harzianum), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCC NO:5812; (2) muck particle preparation process, in described muck particle preparation process, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then by granulation and the dry muck particle that obtains; (3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.
Wherein, the physiological status of described trichoderma strain is at least one in mycelium, chlamydospore and conidium.
Wherein, the operation of described liquid submerged fermentation can be carried out according to conventional liquid submerged fermentation method, under preferable case, the operation of described liquid submerged fermentation comprises the steps: (1) inoculation step, in described inoculation step, the conidium of trichoderma strain as above and/or mycelium are inoculated in liquid nutrient medium; (2) fermentation step in described fermentation step, ferments conidium and/or the mycelium liquid substratum of having inoculated described trichoderma strain in fermentor tank.
Wherein, the conidium of described trichoderma strain and/or mycelium can be used conventional slant culture to obtain, for example, the mycelium of trichoderma strain as above can be seeded in to PDA substratum and (contain potato 180-220g/L, glucose 18-22g/L, agar 10-25g/L) on, at 27-29 ℃, cultivate 5-7 days, can obtain being attached in a large number conidium and/or mycelium on substratum, the substratum that slant culture can be finished digs piece to obtain conidium and/or the mycelium for inoculating.Wherein, the substratum that also slant culture can be finished digs piece and is inoculated in enlarged culturing in liquid nutrient medium, obtain conidium and/or the mycelium of enlarged culturing, using the conidium of enlarged culturing and/or mycelium as the conidium for inoculating and/or mycelium.
Wherein, the condition of described fermentation can be conventional liquid submerged fermentation condition, and under preferable case, the condition of described fermentation comprises: the leavening temperature fermenting 0-40 hour is 27.5-28.5 ℃; The 40th hour later leavening temperature that ferment is 25.5-26.5 ℃; The air flow fermenting 0-10 hour is 1:0.55-0.65 volume: (volume minute); The air flow fermenting 10-40 hour is 1:0.9-1.1 volume: (volume minute), and the 40th hour later air flow that ferment is 1:0.55-0.65 volume: (volume minute); Stirring velocity is 175-185rpm.
Wherein, described liquid nutrient medium can be the substratum of the liquid submerged fermentation that is applicable to Trichoderma of routine, as the substratum of being recorded in ZL200610057339.0, under preferable case, the starch that described liquid nutrient medium contains the material being mixed to get by following weight percent: 2-3.5%, the yeast powder of 1-2%, the corn steep liquor of 4-8%, the CaCO of 0.1-0.5% 3, 0.01-0.02% Zn 2+, 0.01-0.03% Mg 2+water with surplus.
Wherein, in order further to improve chlamydosporic output, the condition of described fermentation also comprises: at fermentation 60-63 hour, with respect to the fermented liquid of every 100 liters, add the starch of 0.45-0.55kg.
Wherein, the total time that described fermentation is carried out can be for carrying out the conventional time adopting of Trichoderma fermentation, under preferable case, the fermentation that finishes for about 100-120 hour in fermentation, now, more than 90% mycelium has all formed chlamydospore, thereby can further improve chlamydosporic output.
Wherein, after finishing fermentation, can by filtering, centrifugal and dry etc., method is separated from finish the fermented liquid of fermentation obtain chlamydospore, also can be directly using the fermented liquid that finishes fermentation as containing chlamydosporic product use.
Wherein, described muck particle can be the various muck particles of routine use, for example the condition of described muck particle preparation process comprises: the water of the humic acid of the chicken manure of 200-400kg, 600-800kg and 200-600kg is mixed, and inoculate after deodorizing bacterial classification 90-110g at 15-40 ℃ of bottom fermentation deodorizing 9-11 days, then cylinder granulation and dry after the muck particle of the diameter 4-5mm that obtains.Wherein, described deodorizing bacterial classification can be the routine various bacterial classifications for deodorizing that use, that can be commercially available.For example, can use purchased from the BM microbial inoculum-starter of Bao Rong bio tech ltd, Henan or purchased from the quick rotting agent of Zhengzhou family estate biotechnology company limited.Wherein, the weight of deodorizing bacterial classification is calculated with dry weight.
Wherein, the operation that described chlamydospore is mixed with muck particle can be conventional married operation; mixed material can be used as microbial organic fertilizer and uses; in order to improve homogeneity and the storage property of mixed material; under preferable case; the operation of described mixing step can comprise: the chlamydospore of Trichoderma kind of the present invention of the muck particle of 900-1100kg, 0.8-1.2kg and the malt sugar of 1-3kg are carried out to dressing at not higher than the temperature of 60 ℃, obtain dressing product.Resulting dressing product has good homogeneity and storage property, can be used as preferred microbial organic fertilizer and uses.Wherein, under preferable case, the chlamydospore quantity in every gram of microbial organic fertilizer is no less than 10 5individual chlamydospore.
The present invention also provides the purposes of microbial organic fertilizer as above in controlling plant diseases.The Plant diseases that microbial organic fertilizer provided by the invention can be prevented and treated other Trichoderma of routine all plays the effect of effective control.Preferably, microbial organic fertilizer provided by the invention has better prevention effect to soil biography Plant diseases; Particularly preferably, microbial organic fertilizer provided by the invention has better prevention effect to cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight.That is, preferred described soil biography Plant diseases is at least one in cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight.
Wherein, prevent and treat the operation of disease and can carry out according to the operation that utilizes Trichoderma to prevent and treat disease of routine, for example, described microbial organic fertilizer directly can be applied to soil and prevent and treat disease.
Wherein, when described microbial organic fertilizer is applied to field, with chlamydosporic amount, calculate, amount of application can in very large range change, for example, can be 5 * 10 4to 2 * 10 5individual chlamydospore/square metre.
Below will describe the present invention by embodiment.
Embodiment 1
Get potato 20g after peeling and boil and within 20 minutes, get juice, juice and 2g glucose, 1.5g agar and water are made into 100ml, the cooling PDA substratum that obtains after bottling sterilizing.
By the CaCO of the corn steep liquor of the yeast powder of the starch of 195kg, 130kg, 325kg, 19.5kg 3, 2.4kg(is so that Zn 2+be 0.015 % by weight) ZnSO 4, 6.5kg MgSO 4(so that Mg 2+be 0.02 % by weight), add water to 6.5m 3, mix and sterilizing, obtain liquid nutrient medium.
By preserving number, be that CGMCC NO:5812 trichoderma harzianum strain is inoculated in PDA substratum, cultivate 6 days at 28 ℃; By inoculate and cultivate after PDA substratum dig piece and be inoculated in shaking flask liquid nutrient medium (the lawn 1cm of 100ml liquid nutrient medium inoculation PDA substratum growth 2), under the condition of 28 ℃ and rotating speed 200rpm, cultivate 24 hours, as seed fermenter inoculum; By in the seed tank culture base after cultured shaking flask inoculum access sterilizing (inoculum size 2%(v/v)), under the condition of 28 ℃ and rotating speed 180rmp, cultivate 24 hours, become ferment-seeded; Ferment-seeded is accessed and in fermentation tank culture medium, carries out controlled fermentation (inoculum size 10%(V/V)).Fermentation condition in fermentor tank is: the leavening temperature fermenting 0-40 hour is 28 ℃; The 40th hour later leavening temperature that ferment is 26 ℃; The air flow fermenting 0-10 hour is 1:0.6 volume: (volume minute); The air flow fermenting 10-40 hour is 1:1 volume: (volume minute), and the 40th hour later air flow that ferment is 1:0.6 volume: (volume minute); Stirring velocity is 180rpm.
In the fermenting process of fermentor tank, carry out feed supplement.In fermentation, in the time of the 62nd hour, with respect to the fermented liquid of every 100 liters, add the starch of 0.5kg.
Ferment the 110th hour time, 90% above mycelia all forms chlamydospore, and fermented liquid is thinning, by fermented liquid press filtration now dry by collecting the screening that press filtration obtains, obtains chlamydospore.This chlamydospore is carried out to microscopic morphology observation, confirm that this chlamydospore has the chlamydospore form of typical trichoderma harziarum.
The water of the humic acid of the chicken manure of 300kg, 700kg and 330kg is mixed, and inoculate after deodorizing bacterial classification (purchased from the BM microbial inoculum-starter of Bao Rong bio tech ltd, Henan) 100g 15-40 ℃ of bottom fermentation deodorizing 10 days, then cylinder granulation and dry after the muck particle of the diameter 4.5mm that obtains.
The chlamydospore of trichoderma harziarum and the malt sugar of 1-3kg that the muck particle that the present embodiment of 900-1100kg is obtained, the present embodiment of 0.8-1.2kg obtain carry out dressing at not higher than the temperature of 60 ℃, obtain dressing product.
Comparative example 1
Adopt the method for embodiment 1 to prepare dressing product, different, the trichoderma harziarum bacterial classification that trichoderma harziarum bacterial classification used is ACCC30371 for the trade names purchased from Chinese agriculture microbial preservation administrative center.
Test implementation example 1
The dressing product that embodiment 1 and comparative example 1 are obtained, respectively as microbial organic fertilizer 1 and microbial organic fertilizer 2, calculates with chlamydosporic amount, and amount of application is 1 * 10 5individual chlamydospore/square metre; inorganic fertilizer (is executed to urea 21kg for every mu; calcium superphosphate 52kg; vitriolate of tartar 11kg) as conventional fertilizer application; according to document, (utilize Trichoderma control cotton verticillium wilt; plant protection journal; the 30th volume; the 3rd phase in 2003; 284-288 page) method in; in 2010, in Shihezi Area of Xinjiang, contrasted microbial organic fertilizer 1, microbial organic fertilizer 2 and conventional fertilizer application on the prevention effect of cotton verticillium wilt, on the impact of output of cotton with on cotton quality impact, result is as shown in table 1-3.Result proves, microbial organic fertilizer of the present invention can improve Trichoderma significantly to be prevented and treated the effect of disease and improve the yield and quality of cotton.
The preventive and therapeutic effect of table 1 microbial organic fertilizer to cotton verticillium wilt
Table 2: the impact of microbial organic fertilizer on output of cotton
Table 3: microbial organic fertilizer affects cotton quality, suede long (cm)
Fertilising On average (measure for 10 times)
Microbial organic fertilizer 1 33.95
Microbial organic fertilizer 2 32.5
Conventional fertilizer application 29.5
Test implementation example 2
The dressing product that embodiment 1 and comparative example 1 are obtained, respectively as microbial organic fertilizer 1 and microbial organic fertilizer 2, calculates with chlamydosporic amount, and amount of application is 5 * 10 4individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document (GB/T 17980.92-2004, bactericidal agent for preventing and treating cotton yellow, blight) in method, microbial organic fertilizer 1, microbial organic fertilizer 2 and the prevention effect of conventional fertilizer application to cotton wilt have been contrasted, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the disease index of cotton wilt has on average declined 28%, microbial organic fertilizer 1 is compared with conventional fertilizer application, and the disease index of cotton wilt has on average declined 52%.Result proves, microbial organic fertilizer of the present invention can improve the effect that Trichoderma prevents and treats disease significantly.
Test implementation example 3
The dressing product that embodiment 1 and comparative example 1 are obtained, respectively as microbial organic fertilizer 1 and microbial organic fertilizer 2, calculates with chlamydosporic amount, and amount of application is 2 * 10 5individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document (trichoderma harziarum and the control of derosal compound use to rice seedling damping-off, journal of Zhejiang university (agricultural and life science version), the 35th volume, 65-70 page) method in, contrasted microbial organic fertilizer 1, microbial organic fertilizer 2 and the conventional fertilizer application prevention effect to rice seedling blight, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the relative control effect of rice seedling blight has on average improved 11%, microbial organic fertilizer 1 is compared with conventional fertilizer application, the relative control effect of rice seedling blight has on average improved 40%.Result proves, microbial organic fertilizer of the present invention can improve the effect that Trichoderma prevents and treats disease significantly.
Test implementation example 4
The dressing product that embodiment 1 and comparative example 1 are obtained, respectively as microbial organic fertilizer 1 and microbial organic fertilizer 2, calculates with chlamydosporic amount, and amount of application is 4 * 10 5individual chlamydospore/square metre, using inorganic fertilizer as conventional fertilizer application, according to document (the control test of biological pesticide " vegetables obtain health " to banana blight, Jiangsu agricultural sciences, the 39th volume, 199-201 page) method in, microbial organic fertilizer 1, microbial organic fertilizer 2 and the prevention effect of conventional fertilizer application to banana blight have been contrasted, wherein microbial organic fertilizer 1 is compared with microbial organic fertilizer 2, the relative control effect of banana blight has on average improved 15%, microbial organic fertilizer 1 is compared with conventional fertilizer application, and the relative control effect of banana blight has on average improved 50%.Result proves, microbial organic fertilizer of the present invention can improve the effect that Trichoderma prevents and treats disease significantly.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.

Claims (10)

1. a preparation method for microbial organic fertilizer, the method comprises the steps:
(1) chlamydospore preparation process, in described chlamydospore preparation process, a kind of trichoderma strain is carried out to liquid submerged fermentation to produce chlamydospore, wherein, this trichoderma strain is the bacterial strain of trichoderma harziarum (Trichoderma harzianum), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO:5812;
(2) muck particle preparation process, in described muck particle preparation process, mixes ight soil, humic acids and water, and inoculates deodorizing bacterial classification and carry out fermentative deodorizing, then by granulation and the dry muck particle that obtains;
(3) mixing step, in described mixing step, mixes described muck particle with described chlamydospore.
2. preparation method according to claim 1, wherein, the operation of described liquid submerged fermentation comprises the steps:
(1) inoculation step, in described inoculation step, is inoculated into the conidium of described trichoderma strain and/or mycelium in liquid nutrient medium;
(2) fermentation step, in described fermentation step, conidium and/or the mycelial liquid nutrient medium of having inoculated described trichoderma strain are fermented in fermentor tank, and the condition of described fermentation comprises: the leavening temperature fermenting 0-40 hour is 27.5-28.5 ℃; The 40th hour later leavening temperature that ferment is 25.5-26.5 ℃; The air flow fermenting 0-10 hour is 1:0.55-0.65 volume: (volume minute); The air flow fermenting 10-40 hour is 1:0.9-1.1 volume: (volume minute), and the 40th hour later air flow that ferment is 1:0.55-0.65 volume: (volume minute); Stirring velocity is 175-185rpm.
3. preparation method according to claim 2, wherein, the starch that described liquid nutrient medium contains the material being mixed to get by following weight percent: 2-3.5%, the yeast powder of 1-2%, the corn steep liquor of 4-8%, the CaCO of 0.1-0.5% 3, 0.01-0.02% Zn 2+, 0.01-0.03% Mg 2+water with surplus.
4. according to the preparation method described in claim 2 or 3, wherein, the condition of described fermentation also comprises: at fermentation 60-63 hour, with respect to the fermented liquid of every 100 liters, add the starch of 0.45-0.55kg.
5. preparation method according to claim 1, wherein, the condition of described muck particle preparation process comprises: the water of the humic acid of the chicken manure of 200-400kg, 600-800kg and 200-600kg is mixed, and inoculate after deodorizing bacterial classification 90-110g at 15-40 ℃ of bottom fermentation deodorizing 9-11 days, then cylinder granulation and dry after the muck particle of the diameter 4-5mm that obtains.
6. preparation method according to claim 1 or 5, wherein, the operation of described mixing step comprises: the malt sugar of the described chlamydospore of the muck particle of 900-1100kg, 0.8-1.2kg and 1-3kg is carried out to dressing at not higher than the temperature of 60 ℃.
7. the microbial organic fertilizer obtaining according to the preparation method described in any one in claim 1-6.
8. the purposes of microbial organic fertilizer claimed in claim 7 in controlling plant diseases.
9. purposes according to claim 8, wherein, described Plant diseases is that soil passes Plant diseases.
10. purposes according to claim 9, wherein, it is at least one in cotton verticillium wilt, cotton wilt, rice seedling blight and banana blight that described soil passes Plant diseases.
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CN103980047A (en) * 2014-05-19 2014-08-13 中国水稻研究所 Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof
CN104230409A (en) * 2014-09-16 2014-12-24 南京农业大学 Growth promoting bio-organic fertilizer and preparation method thereof
CN104446895A (en) * 2014-11-10 2015-03-25 张常书 Trichoderma biological humic acid and product thereof
CN104761385A (en) * 2015-04-11 2015-07-08 黑龙江八一农垦大学 Multifunctional microbial organic fertilizer and preparation method thereof
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CN108929166A (en) * 2018-08-14 2018-12-04 上海绿乐生物科技有限公司 A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism
CN108967095A (en) * 2018-08-14 2018-12-11 文山市泽禄生物科技有限公司 A kind of method that Radix Notoginseng is continuously cultivated
CN110078551A (en) * 2019-05-10 2019-08-02 广西壮族自治区农业科学院 A kind of preparation method and applications of anti-disease microbial bacterial manure

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CN103910545B (en) * 2014-03-18 2016-05-18 福建省农业科学院农业生物资源研究所 Utilize pig manure discarded object to produce the method for Trichoderma harzianum bacterial manure
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CN103947563A (en) * 2014-04-04 2014-07-30 上海德庄农业种植有限公司 Stereo movable sheep collectively-breeding system
CN103980047A (en) * 2014-05-19 2014-08-13 中国水稻研究所 Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof
CN103980047B (en) * 2014-05-19 2016-05-04 中国水稻研究所 Microbial manure of a kind of water resistant rice sprouts damping-off and preparation method thereof
CN104230409A (en) * 2014-09-16 2014-12-24 南京农业大学 Growth promoting bio-organic fertilizer and preparation method thereof
CN104446895A (en) * 2014-11-10 2015-03-25 张常书 Trichoderma biological humic acid and product thereof
CN104761385A (en) * 2015-04-11 2015-07-08 黑龙江八一农垦大学 Multifunctional microbial organic fertilizer and preparation method thereof
CN105211106A (en) * 2015-10-26 2016-01-06 广西南宁益土生物科技有限责任公司 A kind of granule containing trichoderma harzianum and humus
CN105211106B (en) * 2015-10-26 2018-04-24 广西南宁益土生物科技有限责任公司 A kind of granule containing trichoderma harzianum and humic acid
CN108929166A (en) * 2018-08-14 2018-12-04 上海绿乐生物科技有限公司 A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism
CN108967095A (en) * 2018-08-14 2018-12-11 文山市泽禄生物科技有限公司 A kind of method that Radix Notoginseng is continuously cultivated
CN110078551A (en) * 2019-05-10 2019-08-02 广西壮族自治区农业科学院 A kind of preparation method and applications of anti-disease microbial bacterial manure

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