A kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen
Technical field
The invention belongs to agricultural plant field of planting, and in particular to the method and reagent set of a kind of preventing and treating Citrus Huanglongbing pathogen
Dress.
Background technology
Yellow twig is a kind of extensive widespread and very harmful citrus disease, is occurred more than 40 countries and regions extensively,
It is mainly distributed on Asia, Oceania, Africa and America.The U.S., Florida State, the main base of citrus production, yellow twig are tight
Break out again, about more than 50% mandarin tree has been infected Citrus Huanglongbing pathogen.Yellow twig cause of disease is by diaphorina citri Diaphorina
What citri was propagated, pathogen is primarily present in the bast of mandarin tree, and as a result such as yellow arteries and veins hardening symptom, mandarin tree blade occurs
Mottled and yellow, is considered as sometimes that zinc or magnesium lack.The classical symptom of plant of catching an illness includes yellow arteries and veins hardening, patch and spot
Point, Chlorisis index and fruit are small, and roots blacking decayed fruit can not be ripe, and fruit is bitter and acid, and economic worth is substantially reduced,
Huge economic losses are caused to orchard worker.Surgerying yellow twig disease tree, chemical pesticide at present and preventing diaphorina citri and cultivate nontoxic seedling is
The main prophylactico-therapeutic measures that control citrus yellow shoot disease occurs, worldwide, yellow twig is up to the loss that citrus fruit agricultural production is given birth to
It is up to ten million, Citrus Industry is greatly threaten, which results in the strong interest of countries in the world government.
In China, yellow twig morbidity is on the rise, to find out its cause, a large amount of use of oranges and tangerines orchard middling speed effect nitrogenous fertilizer causes soil
Earth acidifying is to cause yellow twig to break out dynamics one of the main reasons above-mentioned year by year, is shown according to data survey, most of oranges and tangerines product
Planting needs preferably be grown under the conditions of pH5-7, and the usage amount of oranges and tangerines orchard nitrogenous fertilizer about per hectare 525 is public in recent years
Jin, and increases year by year, compared to 30 years before usage amount as many as double, excessively cause the acidifying of oranges and tangerines orchard soil using nitrogenous fertilizer,
The Citrus Orchard soil pH in Guangxi, Jiangxi and Hunan is 4.5-5.5, and this leverages the growing environment of mandarin tree;On the other hand,
Research shows that mandarin tree is 6 to the Optimal pH of the absorption of nitrogen, iron, zinc, and B.In acid condition(Below pH5.0), B, Zn, Mg
Element can not be efficiently absorbed, and the element needed for oranges and tangerines can not effectively absorb so as to cause Citrus leaf yellow, live by root system
Power decline, cause development of plants poor, and reduce from soil nutriment absorption, so as to cause Citrus Huanglongbing pathogen usually with
Nutritional deficiency disease occurs simultaneously.Above-mentioned many reasons cause Citrus Huanglongbing pathogen to be on the rise, and the loss that it brings also rapidly is increased, in
State Jiangxi, the mandarin tree that orchard has 30% is infected by yellow twig, and requirement is surgeried.In Hunan China, yellow twig causes huge
Loss reached 30-50%.
At present, it is to chop at a tree, kill wood louse and nontoxic seedling, but these measures to prevent or control the main method of citrus yellow shoot disease
All kinds of corrupt practices creep in for tool, chops at a tree and on the one hand cause huge economic losses to orchard worker, and can not effectively eradicate yellow twig and pathogenic bacteria,
Also there is secondary pollution;Kill wood louse and use many chemical insecticides, environment, plant and fruit are made a very bad impression, sternly
Food security is influenceed during weight, and fruit plant for having caught an illness has little effect, economic loss is still very big;Nontoxic seedling was in recent years
It is heated handful, is a wonderful news of Citrus Industry, but nontoxic seedling is not disease-resistant, it is nontoxic disease-free when garden is gone out only to represent nursery stock, to planting
The requirement for training environment is very high, can not effectively prevent and treat the infection of yellow twig.With the depth that people are understood yellow twig pathogenic factor
Enter, the conventional method that soil pH value is improvement soil acidification is adjusted with quick lime.The patent document of the B of CN 104255351 is disclosed
A kind of method for preventing and treating Citrus Huanglongbing pathogen, it is 5.5~7.5 that soil pH value is carried out disinfection and adjust with quick lime, and cooperation is adopted
With organic water soluble fertilizer, the mixed liquor containing chitin, the foliar fertilizer of rare earth element and organosilicon containing chitin and rare earth
Improvement Citrus Huanglongbing pathogen is carried out with the bio-fertilizer containing bacillus, wherein involved fertilizer is many, complex steps, by
The improvement of year, morbidity tree body only half is got well, and its regulation effect is poor, puts into and is differed with the economic loss retrieved
It is very few, it is unsuitable for spread.
Therefore, the method and product for developing preventing and treating Citrus Huanglongbing pathogen that is new, working well are to save oranges and tangerines industry urgently
The technical problem of solution.
The content of the invention
The technical problem humble to solve current yellow twig prevention and controls scarcity, complex operation, effect, the present invention provides one
The method and reagent set of preventing and treating Citrus Huanglongbing pathogen are planted, soil aciditiy is adjusted by with quick lime, with the use of organic-biological bacterium
Fertilizer, improved soil structure and quality, further coordinate plant immune derivant to promote oranges and tangerines plant root to live by its biological bacteria
Power, improve oranges and tangerines disease resistance so that efficiently, green preventing and treating Citrus Huanglongbing pathogen, promote oranges and tangerines plant healthy growth.
In order to solve the above technical problems, the technical solution adopted by the present invention one is:
A kind of method for preventing and treating Citrus Huanglongbing pathogen, the described method comprises the following steps;
A, the soil applied quick lime according to 50-150kg/ mus of usage amount to oranges and tangerines orchard;
Apply the organic fertilizer containing microbial bacteria in B, the soil according to 100-250kg/ mus of usage amount to oranges and tangerines orchard;
C, per 30-40 days to oranges and tangerines plant spraying or pouring root plant immune derivant once, usage amount 100-150g/ mus, continuously
Using 3-5 times.
Preferably, quick lime is applied in the step A to the soil in oranges and tangerines orchard, applying once improves soil pH value
0.5-1.0, applying finally makes soil pH value improve to 6.0-6.5 twice.
Preferably, when applying the organic fertilizer containing microbial bacteria in soil of the step B to oranges and tangerines orchard, by micro- life
Thing bacterium 0.5-1.5 × 109Cfu/ mus forms added to stirring in organic fertilizer.
Preferably, the microbial bacteria includes Trichoderma and Bacillus laterosporus, the Trichoderma and Bacillus laterosporus
Quantity ratio be 1:1-3.
Preferably, the plant immune derivant is the wettable of active ingredient toVerticilliumdahliaActivitie Activitie S of Relative activator protein content 3wt.%
Property pulvis.
The present invention also provides a kind of reagent set for preventing and treating Citrus Huanglongbing pathogen, and the kit includes following mass fraction
Reagent, reagent one is quick lime, and reagent two is the organic fertilizer containing bacillus, and reagent three is plant immune derivant,
Wherein, the quality parts ratio of the reagent one, reagent two and reagent three is 5-40:10-25:0.03-0.1.
Preferably, microbial bacteria content is 0.5-1.5 × 10 in the organic fertilizer containing microbial bacteria9cfu/100-
250kg。
Preferably, the microbial bacteria includes Trichoderma and Bacillus laterosporus.
More there is choosing, the microbial bacteria is 1 by quantity ratio:The Trichoderma of 1-3 and Bacillus laterosporus are constituted.
Preferably, the plant immune derivant is the wettable of active ingredient toVerticilliumdahliaActivitie Activitie S of Relative activator protein content 3wt.%
Property pulvis.
In above-mentioned technical proposal, there is provided a kind of method and supporting reagent set for preventing and treating Citrus Huanglongbing pathogen, the examination
Agent suit includes the reagent of following mass fraction, and reagent one is quick lime, and reagent two is the organic fertilizer containing microbial bacteria,
Reagent three is plant immune derivant, and the quality parts ratio of the reagent one, reagent two and reagent three is 5-40:10-25:
0.03-0.1.When being used to prevent and treat yellow twig using the reagent set, first, according to 50-150kg/ mus of usage amount by quick lime
Apply to the soil in oranges and tangerines orchard, applying quick lime can improve the situation of soil acidification, by soil pH by less than 5 improve to
6.0-6.5, can lift root activity, promote effective absorption of nutriment in B, Zn, Mg element and soil, be plant immune
The foundation of system provides basis, promotes Citrus leaf to turn green by Huang, so as to improve and alleviate the symptom of yellow twig plant.Further
, the organic fertilizer containing microbial bacteria is applied in the soil according to 100-250kg/ mus of usage amount to oranges and tangerines orchard, pass through
Applying the organic fertilizer containing microbial bacteria can improve the hardened structure of soil, the soil organism and fertility be improved, so as to change
The growing environment of kind root system of plant, greatly improves the root activity of plant, on the other hand can also adjust edaphon flora and put down
Weighing apparatus, suppresses pernicious bacteria growth so that promote plant growth.Lured to the spraying of oranges and tangerines plant or pouring root plant immune per 30-40 days
Lead agent once, usage amount 100-150g/ mus, continuous to use 3-5 times, applying plant immune derivant can promote plant to produce water
The material such as poplar acid, jasmonic and ethene, and then promote antibacterials such as protective plant protecting agent, disease-resistance-related protein and phenol organic matter, antiviral
The synthesis of material, greatly improves the protection capability of plant immune system, obtains system resistant, additionally it is possible to promote plant growth,
Metabolism, improves chlorophyll content of plant, reaches the effect for improving Genes For Plant Tolerance multiple diseases immunocompetence.Above-mentioned three kinds of measure synchronizations
Carry out, mutual cooperation synergy, soil acidification degree is improved by quick lime, lift plant root vigor, coordinate and apply to contain micro- life
The organic fertilizer and applying plant immune derivant of thing bacterium, suppress to form fertile soil basis while pernicious bacteria, enter one
Step promotes plant growth with flourishing Root Absorption trace element and nutriment, promotes plant chlorophyll to increase, and what is absorbed is micro-
Secondary element and nutriment are in the presence of plant immune derivant so that the defence capability of plant immune system is greatly improved,
Plant disease resistance is enhanced, three kinds of measures are complemented each other, preventing and treating is combined, and is treated both principal and secondary aspect of disease, quickly extensive so as to reach infected plant
Multiple normal, risk of catching an illness plant high is from infection.
The beneficial effects of the invention are as follows:(1)Method and reagent set that the present invention is provided, substantially improve soil acidification journey
Degree, have adjusted soil flora, improve plant root vigor and immunocompetence, and that treats both principal and secondary aspect of disease causes infected plant efficiently, soon
The plant that quick-recovery is normal, risk of catching an illness is high improves the cure rate of infected plant from infection, reduces the morbidity of yellow twig
Rate;(2)The inventive method is easily operated, and step is simple, and is green, organic to environment, to plant and fruit nonstaining property
, product quality, quality and food security and Environmental security has been effectively ensured, it is suitable to promote the use of on a large scale.
Brief description of the drawings
Fig. 1 be in embodiment 2 after first time spray on plants immune inducing agent 15 days blank control group and each experimental group
Determine root activity datagram;
Fig. 2A is the oranges and tangerines plant growth condition figure for infecting yellow twig(The sunshine of April 10 in 2016);
The method that Fig. 2 B are provided to infect the oranges and tangerines plant of yellow twig in Fig. 2A with the present invention is processed, in its second spray on plants
The upgrowth situation figure of 15 days after immune inducing agent;
The method that Fig. 2 C are provided to infect the oranges and tangerines plant of yellow twig in Fig. 2A with the present invention is processed, and third time spray on plants is immunized
The upgrowth situation figure of 15 days after derivant.
Specific embodiment
The present invention provides a kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen, below with specific embodiment specifically
Bright, to be better understood from the present invention, involved reagent unless otherwise specified, is commercially obtained, involved operation
Step, unless otherwise specified, is routine operation.
Embodiment 1
The present invention provides a kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen, and the reagent set includes following mass parts
Several reagents, reagent one is quick lime, and reagent two is the organic fertilizer containing microbial bacteria, and reagent three is induced for plant immune
Agent, soil aciditiy and raising plant root vigor are adjusted by with quick lime, with the use of the organic fertilizer containing microbial bacteria,
One side improved soil structure and quality, also adjust colony balance, suppress pernicious bacteria, further coordinate by its biological bacteria and plant
Thing immune inducing agent promotes oranges and tangerines plant root vigor, improves the disease resistance of oranges and tangerines fruit tree immune system, thus efficiently, green it is anti-
Citrus Huanglongbing pathogen is controlled, promotes oranges and tangerines plant healthy growth.
Specifically, the quick lime of the reagent one, organic fertilizer of the reagent two containing microbial bacteria and the plant immune of reagent three
The quality parts ratio of derivant is 5-40:10-25:0.03-0.1.Wherein, organic fertilizer can for chicken manure, cattle and sheep horsehit or dregs of beans,
Cotton dregs etc., general control its composition in organic matter account for inorganic micro micro- element such as 35-45wt.%, moisture 30-40 wt.%, N P and K
20-30 wt.%。
Microbial bacteria content is 0.5-1.5 × 10 in organic fertilizer containing microbial bacteria9Cfu/100-250kg, in right amount
Microbial bacteria can improve soil texture and regulation soil colony balance, suppress pernicious bacteria, promote root system of plant to micro
The absorption rate of element and nutriment.
The microbial bacteria includes Trichoderma and Bacillus laterosporus, and the quantity ratio of Trichoderma and Bacillus laterosporus is 1:
1-3.Trichoderma can produce various bioactivators to plant pathogenic fungi, bacterium and insect with antagonism, suppress,
Weaken and exclude the gray mold pathogen in same growing environment, improve the resistance of crops, moreover it is possible to induce host plant to produce
Raw IRCM Self-Defence System IRCM obtains disease resistance, increases the vigor of root and seedling, promotes plant growth and improves agricultural output;Side spore bud
Born of the same parents bacillus secretes a large amount of chitinases, suppresses fungus disease, while decomposing organic matter, promotes the absorption rate of fertilizer, two
The scientific and reasonable proportioning of person, while ensure that the improved effect to soil, suppressing the effect of pernicious bacteria, further promotes fertilizer absorption
Utilization rate, has facilitation to the foundation of plant self immune system.
The plant immune derivant is the wettable powder of active ingredient toVerticilliumdahliaActivitie Activitie S of Relative activator protein content 3wt.%.
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein is a kind of a kind of egg with bioactivity, single stabilization refined from after microbial fermentation
White matter, can stimulate the increase of plant itself enzymatic activity, so as to stimulate the regulation and control of plant own metabolism, stimulate plant rhizome
The growth of leaf, improves chlorophyll content, specifically there is following effect:(1)Promote plant root growth, strengthen Root Absorption ability,
So as to improve crop yield;(2)Pathogen breeding inside and outside plant processed is faced upward, mitigates pest and disease damage;(3)Enhancing plant metabolism,
Promote photosynthesis and reinforcing blade protection film, resist pathogen;(4)Enhancing photosynthesis, improves chemical fertilizer utilization ratio;The opposing party
Face, the protein stability is good, soluble in water, and in pH4 ~ 10 stabilization, high temperature resistant is boiled 30min and still stablized in 100 DEG C of boiling water, from
2d does not produce influence on effect under right illumination, and 2 years stabilizations of storage at normal temperature more ensure that the stability of its effect and be easy to storage,
Dosage form wettable powder, the wherein content of active ingredient toVerticilliumdahliaActivitie Activitie S of Relative activator protein are 3wt.%, in concrete application, I
Unit uses the commodity that the wettable powder is made packaging, the spirit of trade name ata, is dissipated with the 1000-1500 times of moisture of quality
After can be formed stable suspension for spray or pouring root.
The amount and ratio of three kinds of reagents of preparation scientific and reasonable in this reagent set, adjust soil aciditiy and carry with quick lime
Plant root vigor high, with the use of the organic fertilizer containing microbial bacteria, one side improved soil structure and quality are also adjusted
Colony balance, suppresses pernicious bacteria, further coordinates plant immune derivant to promote oranges and tangerines plant root to live by its biological bacteria
Power, the disease resistance for improving oranges and tangerines fruit tree immune system, thus efficiently, green preventing and treating Citrus Huanglongbing pathogen, promote oranges and tangerines plant health
Growth.
The present embodiment of embodiment 2 proves that this method prevents and treats the effect of Citrus Huanglongbing pathogen by detailed experiment on the spot
The inventive method and reagent set prevent and treat the field comparison test of Citrus Huanglongbing pathogen
Selected yellow twig occurring degree is close, the orchard that upgrowth situation is similar, respectively as 4 experimental groups (label be respectively A,
B, C and D) and 1 blank control group(Label CK), 30 plants of fruit trees of every group for the treatment of, above-mentioned five groups are handled as follows simultaneously:
CK groups:Routine Management, especially treatment is not made;
A groups:Quick lime is applied according to 50-150kg/ mus of usage amount to the soil in oranges and tangerines orchard, it is desirable to quick lime sprinkle it is even,
Cover the earth's surface of whole treatment region;
B groups:Apply the organic fertilizer containing microbial bacteria in soil according to from 100-250kg/ mus of usage amount to oranges and tangerines orchard.
Operating method is along fruit tree in the ranks tree crown position(Drip)Deep-cut straight line ditch or annular groove, it is desirable to trench digging depth 30cm, 20- wide
30cm, it is uniform in ditch to apply the organic fertilizer containing microbial bacteria, it is desirable to which that Term Fertilization ensures uniform;
C groups:Sprayed to oranges and tangerines plant according to 100-150g/ mus of usage amount or pouring root plant immune derivant, later every 30-
40 days continuous spray again or pouring root once, it is continuous to use 3 times, it is desirable to spray every time and be to there is the globule to drip with blade face even spraying
It is accurate;
D groups:Organic fertilizer 100-250kg/ mus according to quick lime 50-150kg/ mus, containing microbial bacteria of usage amount makes
Consumption applies to the soil in oranges and tangerines orchard, while being sprayed to oranges and tangerines plant or pouring root plant according to 100-150g/ mus of usage amount
Immune inducing agent, sprayed or plant immune derivant of pouring root again every 30-40 days later, continuous to use 3 times, it is desirable to it is upper
State group difference identical.
After being processed, it is defined by plant immune derivant spraying time, 15 days after being sprayed at the 1st time respectively, is sprayed for the 2nd time
The incidence of each treatment group fruit tree yellow twig is investigated within 15 days after being sprayed within 15 days and the 3rd time after applying, and according to the 2.1 each group state of an illness
Index and prevention effect are counted, and the results are shown in Table 1;15 days each groups randomly select 6 plant according to following after being sprayed at the 3rd time
2.2TTC methods determine root activity.
2.1 each group disease indexs and prevention effect
(1)Citrus Huanglongbing pathogen severity Scaling standard is:
0 grade:Set entirely disease-free;
1 grade:Having 1 ~ 2 tip on fruit tree has mottled Chlorisis index to occur;
3 grades:Part side shoot or major branch have mottled yellow symptom, and symptom branch accounts for less than the 1/3 of full tree;
5 grades:Symptom side shoot or major branch account for less than more than the 1/3 and 2/3 of full tree;
7 grades:Symptom branch is more than 2/3;
9 grades:Full tree is dead.
(2)Computational methods
1) disease index disease index=[∑ (diseased plant numbers at different levels × relative class value)/(investigation total strain number × 9)] × 100
2) prevention effect(%)=(CK1—PT1)/ CK1×100
Note:CK1 represents blank control group disease index;PT1 represents each treatment group disease index.
According to above-mentioned grade scale and computational methods survey data and prevention effect data, to initial disease before spraying
Shape carries out severity Scaling and disease index and calculates, 5 groups of original state be 0 grade 12,1 grade 9,3 grades 6,5 grades 2,7
Level 1 and 9 grades 0, disease index 16.3, as a result such as following table 1.
The different disposal period yellow twig prevention effect data of table 1
From test data as can be seen that by A, B, C, D the group fruit tree after above method treatment, the yellow twig with blank control group
The state of an illness is compared, and has different degrees of mitigation.15 days after spraying for 1st time, the prevention effect of A, B, C, D group is respectively:4.26%,
4.26%, 4.26% and 23.40%, the D group prevention effects of only Combined Treatment are more apparent, and its prevention effect is much higher than individually to be made
With three kinds of sums of measure, reason is that A groups only improve soil pH value, and root activity is not yet replied, and soil depletion, nutrition are deficient in addition
Weary, its prevention effect is not obvious, and after B groups apply containing the equal organic fertilizer of microorganism, soil fertility strengthens, but soil acidity is still very
Greatly, be unfavorable for absorbing, plant resistance against diseases is strong, C group sprayed plants immune inducing agents, immunocompetence is lifted, healthy plant without
Continue to infect, but because soil acidity is big, fertility is weak, and infected plant restoring force is very weak, and prevention effect is not obvious, only at joint
The D groups of reason, increased soil fertility, suppress in the case of pernicious bacteria, improve soil aciditiy and colony balance, greatly improve root system
Vigor, is conducive to absorbing nutriment and lifting immunity to promote plant strain growth, while plant immune derivant is also carried significantly
The resistance against diseases of plant high, so that infected plant takes a turn for the better, from catching an illness, three kinds of measures are mutually coordinated, greatly for healthy plant
Enhance prevention effect greatly.15 days after spraying for 2nd time, the prevention effect of A, B, C, D group is respectively:10.61%, 18.18%,
25.76% and 59.09%, with the extension of the time of improvement, A, B and C group prevention effect that measure is used alone also pull open gap, with
Apply the resistance against diseases that plant immune derivant improves plant self immune system, prevention effect is more quite a lot of, applies fertilizer, improves
The B groups of soil colony balance are taken second place, and to soil pH is improved, do not increase fertility, and the A group prevention effects of plant immune ability are not increased
It is worst, the D groups that three kinds of measures are used in combination, prevention effect is much higher than individually is imitated using three kinds of A, B and C groups of measure
Really, than three groups of prevention effect sums taller 15% or so;15 days after spraying for 3rd time, the prevention effect of A, B, C, D group is respectively:
9.09%, 23.86%, 30.86% and 78.41%, by the improvement of a cycle, wherein, by three kinds of processing mode integrated treatments
Afterwards, final yellow twig prevention effect is up to 78.41%, than A, B and C group prevention effect sum taller 25% or so.
Method determines root activity
(1)TTC(Triphenyltetrazolium chloride)The making of standard curve
(1.1)Prepare solution
The preparation of 0.4%TTC solution:Weigh 0.4g TTC(Triphenyltetrazolium chloride)It is dissolved in 100mL distilled water and stirs
Uniformly;
(1.2)Make standard curve
The volumetric flask that 0.25ml 0.4%TTC solution is transferred to 10ml is measured, a little sodium thiosulfate powder is added, stood after shaking up
Produce the TTF of red(Triphenyl first), then use ethyl acetate(Analysis is pure)Graduation mark is settled to, is shaken up, obtain 0.1%TTF molten
Liquid.0.1%TTF solution 0.25ml, 0.5ml, 1.00ml, 1.50ml, 2.00ml is measured respectively to add into 5 10ml volumetric flasks,
And graduation mark is settled to ethyl acetate respectively, to shake up obtain and contain the μ g of TTF 25,50 μ g, 100 μ g, 150 μ g, 200 μ g respectively
(Concentration is respectively 2.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 15 μ g/mL, 20 μ g/mL)Standard colorimetric series, with TTF solution make
It is blank, the mensuration absorbance under 485nm wavelength draws TTF absorbance standard curves.
(2)Plant root activity is determined
(2.1)The experimental group and blank control group of plant root are set
Experimental group:Weigh root sample 0.5g to be put into culture dish, add by 0.4%TTC solution and phosphate buffer(1/15μ
Mol/L, pH value is 7)The solution 10ml of mixed in equal amounts, is fully immersed in root in solution, and 1-3h is incubated at dark at 37 DEG C,
The sulfuric acid 2ml of 1mol/L is subsequently adding to stop reaction;
Blank control group:From the sulfuric acid that 2ml, 1mol/L are first added before addition root sample unlike experimental group, other operation phases
Together;
(2.2)Root is taken out, is ground together with 3 ~ 4ml of ethyl acetate and a small amount of quartz sand after suck dry moisture, to propose red
TTF.Red extract solution is collected into test tube, with a small amount of ethyl acetate the residual washing-out two to three times of root, examination is moved into the lump
Pipe, finally adds ethyl acetate to make total amount for 10ml, determines the extinction of extract solution at 485nm with spectrophotometer, is made with blank
Reference reads absorbance, and tetrazole also commercial weight is tried to achieve by standard curve, then brings the data obtained into following formula a, obtains tetrazole
Also intensity.
Formula a tetrazole reduction intensities(Mg/g (root fresh weight)/h)=tetrazole also commercial weight(mg)/ [root weight(g)× the time
(h)]。
Last above-mentioned each group does result data with average value, is made tables of data, sees Fig. 1, it is seen then that comprehensive using the inventive method
D groups its root activity for closing treatment is greatly enhanced, and is on the one hand benefited and improvement of the quick lime to soil aciditiy, and two on the one hand, plants
Thing immune inducing agent makes plant itself establish powerful immune system, in addition the facilitation of microbial bacteria and organic fertilizer,
Three kinds of measures cooperate, and benign cycle is mutually improved, and promotes greatly enhancing for plant root, makes plant healthy growth.
As fully visible, the three kinds of measure coordinate effects of method for the preventing and treating Citrus Huanglongbing pathogen that the present invention is provided, make Huanglong's disease fruit
The infected plant patient's condition is substantially improved in the plant in garden, and non-infected plant substantially increases the preventing and treating of orchard yellow twig from catching an illness
Effect.