CN112602511A - Method for inhibiting citrus greening disease in citrus cultivation - Google Patents
Method for inhibiting citrus greening disease in citrus cultivation Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps: A. pouring potassium permanganate solution into the soil growing at the root of the citrus; B. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil; C. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil, and spraying the whole citrus tree with the compound microorganism solution; the treatment frequency of the step C is 1-3 times; D. after 4-10 days, spraying the whole citrus trees with the composite microbial solution; and D, the treatment frequency of the step D is 1-8 times. The invention combines the multiple advantages of soil sterilization, root irrigation of the microbial solution and the traditional Chinese medicine solution and page spraying of the microbial solution, thereby having stable effect; the scheme of the invention is simple, the cost is low, and the invention is convenient for large-area popularization and wide application.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for inhibiting citrus greening disease in citrus cultivation.
Background
Huanglongbing is a widespread and extremely harmful citrus disease that occurs widely in over 40 countries and regions, mainly in asia, oceania, africa, and america. Yellow shoot is a serious outbreak in the united states, florida, the major base of citrus production, and more than about 50% of citrus trees are infected with citrus yellow shoot. At present, cutting off the yellow shoot sick tree, preventing the diaphorina citri by chemical pesticides and culturing non-toxic seedlings are main control measures for controlling the occurrence of the yellow shoot of the citrus, the loss of the yellow shoot to citrus farmers is up to ten million in the world, the citrus industry is greatly threatened, and strong attention of governments of various countries in the world is attracted.
The pathogen of the yellow shoot is transmitted by Diaphorina citri and can occur all the year round, and symptoms can appear in spring, summer and autumn, and are most obvious in autumn and winter. There are three types of yellowing in leaves, mottled yellowing, uniform yellowing, and absent yellowing. The leaves are locally faded after turning green, form mottled yellowing, are very irregular in mottled positions and shapes, are in a fog shape, have no clear boundary, mostly come from veins, bases or edges, and are relatively accurate judgment symptoms; the uniform yellowing mostly occurs after the local fall of the temperature in autumn, new leaves do not turn green after the extracted autumn tip is late autumn, the uniform yellowing gradually occurs, and the uniform yellowing mostly occurs at the periphery, sunny place and top of the crown, so that the symptom is judged accurately. The yellowing caused by lack of elements is not a true deficiency, is caused by partial rot of roots due to the yellow dragon disease, causes the reduction of fertilizer absorption capacity, causes the deficiency of elements in leaves, mainly shows similar symptoms of zinc deficiency and manganese deficiency, and is an auxiliary symptom for identifying the yellow dragon disease.
At present, cutting off the yellow shoot sick trees, preventing the diaphorina citri by chemical pesticides and culturing non-toxic seedlings are main control measures for controlling the generation of the yellow shoot of the citrus. For example, patent document CN108617696A discloses a compound microbial agent for citrus greening disease, which is prepared by compounding bacillus thuringiensis, beauveria bassiana, trichoderma harzianum and actinomyces frankeliensis according to the mass ratio of (1.5-2.5) to (2.5-3.5) to (3-5) to (0.5-1.5). Patent document CN106857141A describes a method for preventing and treating citrus greening disease, which comprises adjusting soil acidity with quicklime, using organic biological bacterial manure to improve soil structure and quality, and further using biological bacteria to cooperate with plant immunity inducer to promote root activity of citrus plants and improve disease resistance of citrus plants, thereby efficiently and greenly preventing and treating citrus greening disease and promoting healthy growth of citrus plants. Patent document CN110839456A discloses a method for ecologically controlling citrus greening disease by using a compound microorganism bacterial agent, which adopts a compound microorganism a (comprising a liquid beauveria bassiana microbial agent, a liquid metarhizium anisopliae microbial agent, a liquid bacillus subtilis microbial agent, a liquid bacillus licheniformis microbial agent, and a liquid bacillus thuringiensis microbial agent) and a compound microorganism B (comprising a liquid trichoderma viride microbial agent, a liquid lilac paecilomyces microbial agent, a liquid bacillus subtilis microbial agent, a liquid bacillus licheniformis microbial agent, and a liquid bacillus thuringiensis microbial agent) to form the compound microorganism bacterial agent for controlling citrus greening disease. However, the existing method has some defects: although the compound microorganism solution of CN108617696A and CN110839456A has a balancing effect on microorganisms in soil, harmful bacteria such as gram-negative bacteria causing yellow dragon disease still have the possibility of causing diseases, outbreaks and infection due to the lack of a step of killing microorganisms; in patent document CN106857141A, quicklime is used to prevent and treat huanglongbing, and when the quicklime is heated in water, it can kill microorganisms only on the surface of the ground, and it is difficult to penetrate into the soil; in CN107372667A, the compound traditional Chinese medicine reagent is sprayed on the surface of a citrus tree to prevent and treat the huanglongbing, the method cannot deeply enter soil to control soil microorganisms, and a solution for controlling germs of the whole tree by the absorption of a plant root system is lacked, so that the germs can continuously cause diseases or outbreaks in the soil, the root system and even a trunk.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a chicken feed and a preparation method thereof.
The purpose of the invention is realized by the following technical scheme:
the invention provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. firstly, filling potassium permanganate solution into soil growing at the root of the citrus;
B. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil;
C. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil, and spraying the whole citrus tree with the compound microorganism solution; the treatment frequency of the step C is 1-3 times;
D. after 4-10 days, spraying the whole citrus trees with the composite microbial solution; and D, the treatment frequency of the step D is 1-8 times.
Preferably, the concentration of the potassium permanganate solution is 0.1-0.3%; the injection amount of the potassium permanganate solution in the step A is 15-35 jin per plant;
the frequency of pouring the potassium permanganate solution into the soil growing at the root of the citrus is 1-3 times; the interval time is 3-5 days each time.
Preferably, in each step, the potassium permanganate solution, the composite microorganism solution and the astragalus membranaceus extracting solution are filled in the following steps: the root of a trunk is taken as a central point, 2-16 uniformly distributed points are selected in a circumference with the radius of 25-100% of the width of the crown, a root irrigation gun is inserted into each point to irrigate roots with a corresponding potassium permanganate solution, a compound microorganism solution and an astragalus membranaceus extracting solution, and the insertion depth of the root irrigation gun is 10-50 centimeters away from the ground.
Preferably, in steps B, C and D, the composite microorganism solution comprises Bacillus subtilis, Bacillus licheniformis and Trichoderma harzianum; wherein: the effective content of the bacillus subtilis is 200-1000 hundred million/g, the effective content of the bacillus licheniformis is 200-1000 hundred million/g, and the effective content of the trichoderma harzianum is 5-50 hundred million/g.
More preferably, the effective content of the bacillus subtilis, the bacillus licheniformis and the trichoderma harzianum is 1000 hundred million/gram, 1000 hundred million/gram and 10 hundred million/gram respectively.
Preferably, the mass ratio of the bacillus subtilis to the bacillus licheniformis to the trichoderma harzianum is 2-10: 1-5, and more preferably 2:1: 1.
Preferably, in steps B, C and D, the mass percentage of the composite microorganisms in the composite microorganism solution is 0.01-0.05%.
Preferably, the filling amount of the compound microorganism solution in the step B is 2-20 jin/strain; the filling amount of the compound microorganism solution in the step C is 2-20 jin/plant, and the spraying amount of the compound microorganism solution in the steps C and D is 2-20 jin/plant.
Preferably, in step B, the preparation method of the astragalus root extract solution is: soaking sliced radix astragali in water, decocting for 2-3 hours, and filtering to obtain radix astragali extract.
Preferably, the mass ratio of the water to the astragalus membranaceus is 100: 1-10.
Preferably, in the steps B and C, the filling amount of the astragalus mongholicus extracting solution is 12-32 jin/plant.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention can kill and inhibit various bacteria in the soil, especially near the root by adding the potassium permanganate solution, so as to ensure that the advantages can be well formed after the subsequent beneficial microorganisms are injected into the soil.
2. The invention can be added with compound microbe to rebuild the microbe system in soil.
3. By adding the radix astragali extract, the nutrient substances and trace elements of the radix astragali extract are extremely rich, so that the beneficial bacteria can be well adapted and grown in soil, and beneficial nutrition can be supplemented to the root system of the citrus so as to help the root system to grow. The compound microbial fertilizer and compound microbial bacteria act together to improve the microbial balance level in soil, enhance the fermentation effect of soil, promote the good development of plant roots and enhance the absorption capacity of the plant roots, so that the whole plant grows well, and the plant morbidity can be controlled below 2%.
4. The invention combines the multiple advantages of soil sterilization, root irrigation of the microbial solution and the traditional Chinese medicine solution and page spraying of the microbial solution, thereby having stable effect; in addition, the scheme of the invention is as simple as possible, has low cost, is convenient for large-area popularization and is widely implemented.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which is implemented 3-4 months before citrus picking, and comprises the following steps:
A. injecting a potassium permanganate solution into the soil for growing the citrus:
a1, preparing a 0.2 percent potassium permanganate solution;
a2 selecting 4 symmetrical points with radius of one fourth of the width of the tree crown at the point with the root of the tree trunk as the center, inserting root-filling guns at each point to fill roots with potassium permanganate solution at the depth of 30 cm from the ground, and filling 6 jin at each point.
B. After about 5 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into roots, and pouring 8 jin into each point position.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
c5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into the roots, and pouring 8 jin into each point position;
c6, spraying the whole tree with the microbial solution, wherein each tree is sprayed with 6 jin.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole trees by 6 jin each tree.
E. Repeat step D every 1 week for 4 replicates.
Example 2
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. and (3) pouring a potassium permanganate solution into the soil where the oranges grow:
a1, preparing a 0.1 percent potassium permanganate solution;
a2, selecting 4 symmetrical points on the circumference of which the radius is one fourth of the width of the tree crown and the point which takes the root of the tree trunk as the center, inserting a root-filling gun at each point to 30 centimeters deep from the ground to fill the roots with the potassium permanganate solution, and filling 6 jin at each point position.
B. After about 5 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-filling gun into the point locations in the step A2 to fill the mixed solution into the roots, and filling 8 jin into each point location.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
c5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into the roots, and pouring 8 jin into each point position.
C6, spraying the whole tree with the microbial solution, wherein each tree is sprayed with 6 jin.
C7: and C1-C62 times.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole tree by 8 jin each tree.
E. Repeat step D every 1 week for 4 replicates.
Example 3
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. and (3) pouring a potassium permanganate solution into the soil where the oranges grow:
a1, preparing a 0.2 percent potassium permanganate solution;
a2, selecting 4 symmetrical points in the circumference of which the radius is one fourth of the width of the tree crown and the point which takes the root of the tree trunk as the center, inserting a root-filling gun at each point to 30 centimeters deep from the ground to fill the root with the potassium permanganate solution, and filling 6 jin at each point position.
B. After about 5 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into roots, and pouring 8 jin into each point position.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
c5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into the roots, and pouring 8 jin into each point position.
C6, spraying the whole tree with the microbial solution, wherein each tree is sprayed with 6 jin.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole trees by 6 jin each tree.
E. Repeat step D every 1 week for 5 replicates.
Example 4
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. and (3) pouring a potassium permanganate solution into the soil where the oranges grow:
a1, preparing a 0.2 percent potassium permanganate solution;
a2, selecting 4 symmetrical points in the circumference of which the radius is one fourth of the width of the tree crown and the point which takes the root of the tree trunk as the center, inserting a root-filling gun at each point to 30 centimeters deep from the ground to fill the root with the potassium permanganate solution, and filling 6 jin at each point position.
B. After about 5 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into roots, and pouring 6 jin into each point position.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
c5, inserting a root-pouring gun into the point positions in the step A2 to pour the mixed solution into roots, and pouring 6 jin into each point position.
C6, spraying the whole tree with 4 jin of microorganism solution.
C7: and C1-C62 times.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 20 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 10 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole tree, wherein each tree is sprayed with 4 jin.
E. Repeat step D every 1 week for 8 replicates.
Example 5
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. and (3) pouring a potassium permanganate solution into the soil where the oranges grow:
a1, preparing a 0.3 percent potassium permanganate solution;
a2, selecting 6 symmetrical points in the circumference with the radius of the crown width and the root-filling gun inserted into each point to fill roots with potassium permanganate solution at the depth of 30 centimeters from the ground, and filling 4 jin at each point.
B. After about 7 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 25 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 5 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-filling gun into the point locations in the step A2 to fill the mixed solution into the roots, and filling 4 jin into each point location.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 25 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 5 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
and C5, inserting a root-pouring gun into the point positions in the step A2 to pour the mixed solution into roots, and pouring 4 jin into each point position.
C6, spraying the whole tree with the microbial solution, wherein each tree is sprayed with 2 jin.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 25 g of bacillus subtilis (with effective content of 1000 hundred million/g), 10 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 5 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole tree, wherein each tree is sprayed with 2 jin.
E. Repeat step D every 1 week for 2 replicates.
Example 6
The embodiment provides a method for inhibiting citrus greening disease in citrus cultivation, which comprises the following steps:
A. and (3) pouring a potassium permanganate solution into the soil where the oranges grow:
a1, preparing a 0.1 percent potassium permanganate solution;
a2, selecting 8 symmetrical points in the circumference of which the radius is one half of the width of the tree crown and the point which takes the root of the tree trunk as the center, inserting a root-filling gun at each point to the ground for filling the root with the potassium permanganate solution at the depth of 40 centimeters, and filling 2 jin at each point position.
B. After about 5 days from the completion of the above steps, the root soil was applied with a composite microorganism solution and an astragalus solution:
b1, mixing 1 kg of water with 10 g of bacillus subtilis (with effective content of 1000 hundred million/g), 20 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 20 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
b5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into roots, and pouring 5 jin into each point position.
C. After five days, the root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and the foliage spraying of the compound microorganism solution is carried out simultaneously:
c1, mixing 1 kg of water with 10 g of bacillus subtilis (with effective content of 1000 hundred million/g), 20 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 20 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking 1 kg of sliced radix astragali in 100 kg of clear water, boiling for 2-3 hours, and fishing out the radix astragali to obtain the radix astragali extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to obtain a mixed solution;
and C5, inserting a root-pouring gun into the point positions of the step A2 to pour the mixed solution into roots, and pouring 5 jin into each point position.
C6, spraying the whole tree with 10 jin of microorganism solution.
D. After five days, the foliar spraying of the compound microorganism solution is carried out again:
d1, mixing 1 kg of water with 10 g of bacillus subtilis (with effective content of 1000 hundred million/g), 20 g of bacillus licheniformis (with effective content of 1000 hundred million/g) and 20 g of trichoderma harzianum (with effective content of 10 hundred million/g) to prepare a compound microorganism solution, dissolving and uniformly mixing;
d2, diluting the compound microorganism solution by 100 times;
d3, spraying the whole trees by 10 jin each tree.
E. Repeat step D every 1 week for 2 replicates.
Comparative example 1
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which basically comprises the following steps of: this comparative example did not undergo the treatment of step a.
Comparative example 2
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which basically comprises the following steps of: in steps B and C, the present comparative example applied only the composite microbial solution to the root soil to irrigate the roots.
Comparative example 3
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which basically comprises the following steps of: step C is omitted, and step D is directly carried out.
Comparative example 4
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which has the following steps basically the same as those in example 6, except that: step B1 is: 1 kg of water is taken and mixed with 10 g of bacillus subtilis, 20 g of bacillus licheniformis and 20 g of beauveria bassiana to prepare a compound microorganism solution; and no astragalus solution is used in steps B and C.
Comparative example 5
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which has the following steps basically the same as those in example 6, except that: step B1 is: 1 kg of water is taken and mixed with 10 g of bacillus subtilis, 20 g of bacillus thuringiensis and 20 g of trichoderma harzianum to prepare a compound microorganism solution; and no astragalus solution is used in steps B and C.
Comparative example 6
The comparative example provides a method for inhibiting citrus greening disease in citrus cultivation, which has the following steps basically the same as those in example 6, except that: step B1 is: 1 kg of water is taken and mixed with 5 g of bacillus subtilis, 20 g of bacillus licheniformis and 20 g of trichoderma harzianum to prepare a compound microorganism solution; and no astragalus solution is used in steps B and C.
Effect verification:
1. after 3-4 months of cultivation of the citrus in the above examples 1-6 and comparative examples 1-6, the planted citrus is tested, and the gloss extension degree of citrus leaves, the taste of the citrus, the yield of the citrus and the like are detected and compared. The specific detection method comprises the following steps:
1) degree of gloss extension of leaves
The method comprises the following steps: observe the gloss extension of the leaves
Evaluation criteria for gloss effect: the glossier the blade, the more spread the better.
2) Citrus taste testing
The method comprises the following steps: and comparing the subjective evaluation of tasting sour sweetness.
And (4) judging the standard: the higher the sweetness, the better, the moderate acidity
3) Average citrus yield statistics
After the oranges are picked, the total weight of the oranges produced by each plant is directly weighed.
The test results of each example group and comparative example group are shown in table 1.
TABLE 1
2. Prevention and treatment effect of citrus greening disease
1) The same treatment method as that of examples 1-6 and comparative examples 1-6 is adopted to compare the control effect of the yellow shoot to 1-2 years of citrus planted in a yellow shoot high-incidence area (the average incidence rate is 2-5%), and the results are shown in table 2.
TABLE 2
2) The citrus fruits having developed yellow shoot were treated by the methods of examples 1 to 6 and comparative examples 1 to 6, and the cure rate results of yellow shoot are shown in table 3.
TABLE 3
| Test group | Cure rate of Huanglong disease (%) | Test group | Cure rate of Huanglong disease (%) |
| EXAMPLE 1 group | 80 | Comparative example 1 group | 20 |
| EXAMPLE 2 group | 70 | Comparative example 2 group | 45 |
| EXAMPLE 3 group | 70 | Comparative example 3 group | 45 |
| EXAMPLE 4 group | 65 | Comparative example 4 group | 40 |
| EXAMPLE 5 group | 60 | Comparative example 5 group | 40 |
| EXAMPLE 6 group | 50 | Comparative example 6 group | 43 |
The invention has many applications, and the above description is only a preferred embodiment of the invention. It should be noted that the above examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that various modifications can be made without departing from the principles of the invention and these modifications are to be considered within the scope of the invention.
Claims (10)
1. A method for inhibiting citrus greening disease in citrus cultivation is characterized by comprising the following steps:
A. pouring potassium permanganate solution into the soil growing at the root of the citrus;
B. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil;
C. after 4-10 days, pouring the compound microorganism solution and the astragalus mongholicus extracting solution into the citrus root soil, and spraying the whole citrus tree with the compound microorganism solution; the treatment frequency of the step C is 1-3 times;
D. after 4-10 days, spraying the whole citrus trees with the composite microbial solution; and D, the treatment frequency of the step D is 1-8 times.
2. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein the concentration of the potassium permanganate solution is 0.1-0.3%; the injection amount of the potassium permanganate solution in the step A is 15-35 jin per plant;
the frequency of pouring the potassium permanganate solution into the soil growing at the root of the citrus is 1-3 times; the interval time is 3-5 days each time.
3. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein the potassium permanganate solution, the composite microbial solution and the astragalus membranaceus extracting solution are poured in the following steps: the root of a trunk is taken as a central point, 2-16 uniformly distributed points are selected in a circumference with the diameter of 25-100% of the width of a crown, a root irrigation gun is inserted into each point to irrigate roots with a corresponding potassium permanganate solution, a compound microorganism solution and an astragalus membranaceus extracting solution, and the insertion depth of the root irrigation gun is 10-50 centimeters away from the ground.
4. The method of inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein in steps B, C and D, said complex microbial solution comprises bacillus subtilis, bacillus licheniformis and trichoderma harzianum; the effective content of the bacillus subtilis is 200-1000 hundred million/g, the effective content of the bacillus licheniformis is 200-1000 hundred million/g, and the effective content of the trichoderma harzianum is 5-50 hundred million/g.
5. The method for inhibiting citrus greening disease in citrus cultivation according to claim 4, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the trichoderma harzianum is 2-10: 1-5.
6. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1 or 4, wherein in steps B and C, the mass percentage of the composite microorganisms in the composite microorganism solution is 0.01 to 0.05%.
7. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein the amount of the compound microorganism solution poured in step B is 2 to 20 jin/plant; c, filling the compound microorganism solution into the tank in an amount of 2-20 jin/plant; the spraying amount of the compound microorganism solution in the steps C and D is 2-20 jin/plant.
8. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein in step B, the method for preparing the astragalus membranaceus extract solution comprises: soaking sliced radix astragali in water, decocting for 2-3 hours, and filtering to obtain radix astragali extract.
9. The method for inhibiting citrus greening disease in citrus cultivation according to claim 8, wherein the mass ratio of water to astragalus membranaceus is 100: 1-10.
10. The method for inhibiting citrus greening disease in citrus cultivation according to claim 1, wherein in steps B and C, the amount of the astragalus membranaceus extract added is 12-32 jin/plant.
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