CN112602511B - Method for inhibiting citrus yellow dragon disease in citrus cultivation - Google Patents
Method for inhibiting citrus yellow dragon disease in citrus cultivation Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, which comprises the following steps: A. filling potassium permanganate solution into soil for citrus root growth; B. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract; C. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract, and the compound microorganism solution is used for spraying the citrus on the whole tree; the treatment times of the step C are 1-3 times; D. after 4-10 days, spraying the citrus full tree with the composite microbial solution; the treatment times of the step D are 1-8 times. The invention combines the advantages of soil sterilization, root irrigation of microbial solution and traditional Chinese medicine solution and page spraying of microbial solution, thus having stable effect; the invention has simple scheme, low cost, convenient large-area popularization and wide application.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for inhibiting citrus yellow dragon disease in citrus cultivation.
Background
The yellow dragon disease pathogen is transmitted by Diaphorina citri, and can occur all the year round, and symptoms can appear in spring, summer and autumn, and the symptoms are most obvious in autumn and winter. The leaves had three types of yellowing, namely mottled yellowing, homogeneous yellowing and anaplastic yellowing. The leaves turn green and then fade locally to form mottled yellowing, the mottled position and shape are very irregular, the mottled position and shape are vaporific, no clear boundary exists, most mottled origin is derived from veins, basal parts or edges, and the mottled position and shape are more accurate judgment symptoms; even yellowing occurs more in autumn after the temperature partially falls back, the newly-grown autumn leaves are not turned green slightly in late autumn, even yellowing is gradually formed, and the even yellowing occurs more at the periphery of the crown, the sunny place and the top, so that the symptom is accurately judged. The element-deficiency yellowing is not true element deficiency, is caused by partial rot of roots caused by the yellow dragon disease, reduces fertilizer absorbing capacity, causes element deficiency of leaves, is mainly similar to zinc deficiency and manganese deficiency symptoms, and is an auxiliary symptom for identifying the yellow dragon disease.
At present, the prevention of diaphorina citri by cutting down yellow dragon disease trees and chemical pesticides and the cultivation of non-toxic seedlings are main prevention and control measures for controlling the occurrence of yellow dragon disease. For example, patent document CN108617696A describes a composite microbial agent for citrus yellow dragon disease, which is prepared by compounding (1.5-2.5): (2.5-3.5): (3-5): (0.5-1.5) by mass ratio of bacillus thuringiensis, beauveria bassiana, trichoderma harzianum and actinomyces fraxinus. Patent document CN106857141A describes a method for preventing and treating citrus yellow dragon disease, which comprises the steps of regulating soil acidity by quicklime, using organic biological bacterial fertilizer in a matching way, improving soil structure and quality, further promoting root system activity of citrus plants by means of biological bacteria and plant immunity inducer, improving disease resistance of citrus plants, thereby preventing and treating citrus yellow dragon disease efficiently and greenly, and promoting healthy growth of citrus plants. Patent document CN110839456a discloses a method for ecologically preventing and treating citrus yellow dragon disease by using a compound microorganism agent, wherein a compound microorganism agent A (comprising a liquid beauveria bassiana agent, a liquid green muscardine fungus agent, a liquid bacillus subtilis fungus agent, a liquid bacillus licheniformis fungus agent and a liquid bacillus thuringiensis fungus agent) and a compound microorganism agent B (comprising a liquid trichoderma viride agent, a liquid lilac paecilomyces agent, a liquid bacillus subtilis fungus agent, a liquid bacillus licheniformis fungus agent and a liquid bacillus thuringiensis fungus agent) are adopted to form the compound microorganism agent for preventing and treating citrus yellow dragon disease. However, the existing methods have some disadvantages: although the complex microorganism solutions of CN108617696a and CN110839456a have a balance effect on microorganisms in soil, there is still the possibility of pathogenicity, outbreak and infection of harmful bacteria such as gram negative bacteria that cause yellow dragon disease due to lack of a step of sterilizing microorganisms; in the patent document CN106857141A, quicklime is used for preventing and treating yellow dragon disease, and the quicklime generates heat when meeting water to kill microorganisms only on the surface of the ground, so that the quicklime is difficult to penetrate into soil; in CN107372667A, a composite traditional Chinese medicine reagent is sprayed on the surface of a citrus tree to prevent and treat the yellow dragon disease, the method cannot go deep into soil to control soil microorganisms, and a solution for controlling germs on the whole tree through plant root system absorption is lacking, so that the possibility of pathogenicity or outbreak of germs in the soil, root systems and even trunks is continued.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for inhibiting citrus yellow dragon disease in citrus cultivation.
The invention aims at realizing the following technical scheme:
the invention provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, which comprises the following steps:
A. firstly, filling potassium permanganate solution into soil for citrus root growth;
B. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract;
C. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract, and the compound microorganism solution is used for spraying the citrus on the whole tree; the treatment times of the step C are 1-3 times;
D. after 4-10 days, spraying the citrus full tree with the composite microbial solution; the treatment times of the step D are 1-8 times.
Preferably, the concentration of the potassium permanganate solution is 0.1-0.3%; the filling amount of the potassium permanganate solution in the step A is 15-35 jin/strain;
the time of filling the potassium permanganate solution into the soil for growing the citrus root is 1-3 times; the time interval is 3-5 days each time.
Preferably, in each step, the filling methods of the potassium permanganate solution, the compound microorganism solution and the astragalus extract are as follows: and taking the root of the trunk as a central point, selecting 2-16 evenly distributed points in the circumference with the radius of 25-100% of the width of the crown, and irrigating the root of the corresponding potassium permanganate solution, the compound microorganism solution and the astragalus root extracting solution by inserting a root irrigating gun into each point, wherein the inserting depth of the root irrigating gun is 10-50 cm away from the ground.
Preferably, in steps B, C and D, the compound microorganism solution includes bacillus subtilis, bacillus licheniformis and trichoderma harzianum; wherein: the effective content of the bacillus subtilis is 200-1000 hundred million/g, the effective content of the bacillus licheniformis is 200-1000 hundred million/g, and the effective content of the trichoderma harzianum is 5-50 hundred million/g.
More preferably, the effective content of the bacillus subtilis, the bacillus licheniformis and the trichoderma harzianum is 1000 hundred million/gram, 1000 hundred million/gram and 10 hundred million/gram respectively.
Preferably, the mass ratio of the bacillus subtilis to the bacillus licheniformis to the trichoderma harzianum is 2-10:1-5:1-5, more preferably 2:1:1.
Preferably, in steps B, C and D, the mass percentage of the composite microorganism in the composite microorganism solution is 0.01-0.05%.
Preferably, the filling amount of the compound microorganism solution in the step B is 2-20 jin/strain; the filling amount of the compound microorganism solution in the step C is 2-20 jin/strain, and the spraying amount of the compound microorganism solution in the steps C and D is 2-20 jin/strain.
Preferably, in the step B, the preparation method of the astragalus extract comprises the following steps: soaking sliced astragalus root in water, boiling for 2-3 hr, and filtering to obtain astragalus root extract.
Preferably, the mass ratio of the water to the astragalus is 100:1-10.
Preferably, in the steps B and C, the filling amount of the astragalus extract is 12-32 jin/plant.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, various bacteria in the soil, especially near the root, can be killed and inhibited by adding the potassium permanganate solution, so that the advantage can be well formed after the follow-up beneficial microorganisms are injected into the soil.
2. The invention further adds compound microorganism bacteria to reestablish a microorganism system in soil.
3. By adding the astragalus extract, the nutrient substances and microelements of the astragalus extract are extremely rich, so that the probiotics can be well adapted and grown in soil, and beneficial nutrition can be supplemented for root systems of oranges to help the root systems grow. The microbial agent acts together with the compound microbial bacteria to improve the microbial balance level in the soil, promote the fermentation of the soil, promote the good development of plant roots and enhance the absorption capacity of the plant roots, so that the whole plant grows well, and the plant morbidity can be controlled below 2%.
4. The invention combines the advantages of soil sterilization, root irrigation of microbial solution and traditional Chinese medicine solution and page spraying of microbial solution, thus having stable effect; in addition, the scheme of the invention is as simple as possible, has low cost, is convenient for large-area popularization and is widely implemented.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
Example 1
The embodiment provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, which is implemented 3-4 months before citrus picking and comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.2% potassium permanganate solution;
a2, selecting 4 symmetrical points with the radius being one fourth of the width of the crown at the point taking the root of the trunk as the center, inserting a root filling gun at each point and filling potassium permanganate solution into the root at a depth of 30 centimeters from the ground, wherein each point is filled with 6 jin.
B. After the above steps are completed for about 5 days, applying a complex microorganism solution and an astragalus solution to the root soil:
b1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 8 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
c5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 8 jin of each point position;
and C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 6 jin.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 6 jin.
E. Step D was repeated 4 times every 1 week.
Example 2
The embodiment provides a method for inhibiting citrus yellow long disease in citrus cultivation, which comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.1% potassium permanganate solution;
a2, selecting 4 symmetrical points on a circumference with the root of the trunk as the center and the radius being one fourth of the width of the crown, inserting a root filling gun into each point and filling the potassium permanganate solution into each point at a depth of 30 centimeters from the ground, and filling 6 jin of potassium permanganate solution into each point.
B. After the above steps are completed for about 5 days, applying a complex microorganism solution and an astragalus solution to the root soil:
b1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun row mixed solution into the point positions in the step A2 to fill roots, and filling 8 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and C5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 8 jin of each point position.
And C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 6 jin.
C7: repeating the steps C1-C6 2 times.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 8 jin.
E. Step D was repeated 4 times every 1 week.
Example 3
The embodiment provides a method for inhibiting citrus yellow long disease in citrus cultivation, which comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.2% potassium permanganate solution;
a2, selecting 4 symmetrical points in the circumference with the root of the trunk as the center and the radius being one fourth of the width of the crown, inserting a root filling gun into each point and filling the potassium permanganate solution into each point at a depth of 30 centimeters from the ground, and filling 6 jin of potassium permanganate solution into each point.
B. After the above steps are completed for about 5 days, applying a complex microorganism solution and an astragalus solution to the root soil:
b1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 8 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and C5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 8 jin of each point position.
And C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 6 jin.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 6 jin.
E. Step D was repeated 5 times every 1 week.
Example 4
The embodiment provides a method for inhibiting citrus yellow long disease in citrus cultivation, which comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.2% potassium permanganate solution;
a2, selecting 4 symmetrical points in the circumference with the root of the trunk as the center and the radius being one fourth of the width of the crown, inserting a root filling gun into each point and filling the potassium permanganate solution into each point at a depth of 30 centimeters from the ground, and filling 6 jin of potassium permanganate solution into each point.
B. After the above steps are completed for about 5 days, applying a complex microorganism solution and an astragalus solution to the root soil:
b1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 6 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and C5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 6 jin of each point position.
And C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 4 jin.
C7: repeating the steps C1-C6 2 times.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 20 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 10 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 4 jin.
E. Step D was repeated 8 times every 1 week.
Example 5
The embodiment provides a method for inhibiting citrus yellow long disease in citrus cultivation, which comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.3% potassium permanganate solution;
a2, selecting 6 symmetrical points in the circumference with the root of the trunk as the center and the radius of the crown, inserting a root filling gun into each point and filling potassium permanganate solution into each point at a depth of 30 cm from the ground, and filling 4 jin of potassium permanganate solution into each point.
B. After the above steps are completed for about 7 days, the root soil is applied with a complex microbial solution and an astragalus solution:
b1, preparing a compound microorganism solution by mixing 1 kg of water into 25 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 5 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun row mixed solution into the point positions in the step A2 to fill roots, and filling 4 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 25 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 5 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and C5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 4 jin of each point position.
And C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 2 jin.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 25 g of bacillus subtilis (the effective content is 1000 hundred million/g), 10 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 5 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 2 jin.
E. Step D was repeated 2 times every 1 week.
Example 6
The embodiment provides a method for inhibiting citrus yellow long disease in citrus cultivation, which comprises the following steps:
A. filling potassium permanganate solution into soil for citrus growth:
a1, preparing 0.1% potassium permanganate solution;
a2, 8 symmetrical points are selected in the circumference with the root of the trunk as the center and the radius being one half of the width of the crown, a root filling gun is inserted into each point and 40 centimeters deep from the ground to fill the root with the potassium permanganate solution, and each point is filled with 2 jin of potassium permanganate solution.
B. After the above steps are completed for about 5 days, applying a complex microorganism solution and an astragalus solution to the root soil:
b1, 1 kg of water is mixed with 10 g of bacillus subtilis (the effective content is 1000 hundred million/g), 20 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 20 g of trichoderma harzianum (the effective content is 10 hundred million/g) to prepare a compound microorganism solution, and the compound microorganism solution is uniformly dissolved and mixed;
b2, diluting the compound microorganism solution by 100 times;
b3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
b4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and B5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 5 jin of each point position.
C. After five days, root irrigation of the compound microorganism solution and the astragalus solution is carried out again to consolidate the soil protection effect, and simultaneously, leaf surface spraying of the compound microorganism solution is carried out:
1, preparing a compound microorganism solution by mixing 1 kg of water into 10 g of bacillus subtilis (the effective content is 1000 hundred million/g), 20 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 20 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
c2, diluting the compound microorganism solution by 100 times;
c3, soaking and slicing 1 kg of astragalus with 100 kg of clear water, boiling for 2-3 hours, and taking out the astragalus, wherein the left astragalus is astragalus extract;
c4, mixing the compound microorganism solution and the astragalus solution according to the mass ratio of 1:1 to form a mixed solution;
and C5, inserting a root filling gun into the point positions in the step A2 to fill the mixed solution into roots, and filling 5 jin of each point position.
And C6, spraying the microbial solution of the whole tree, wherein each tree is sprayed with 10 jin.
D. After five days, the foliar spray of the composite microbial solution was again performed:
1, preparing a composite microorganism solution by mixing 1 kg of water into 10 g of bacillus subtilis (the effective content is 1000 hundred million/g), 20 g of bacillus licheniformis (the effective content is 1000 hundred million/g) and 20 g of trichoderma harzianum (the effective content is 10 hundred million/g), and uniformly dissolving and stirring;
d2, diluting the compound microorganism solution by 100 times;
and D3, spraying the whole tree, wherein each tree is sprayed with 10 jin.
E. Step D was repeated 2 times every 1 week.
Comparative example 1
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 1, except that: the present comparative example does not perform the process of step a.
Comparative example 2
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 1, except that: in steps B and C, the root soil is only irrigated with the compound microorganism solution in this comparative example.
Comparative example 3
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 1, except that: step C is omitted and step D is directly performed.
Comparative example 4
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 6, except that: the step B1 is as follows: 1 kg of water is mixed with 10 g of bacillus subtilis, 20 g of bacillus licheniformis and 20 g of beauveria bassiana to prepare a compound microorganism solution; and astragalus solution is not used in steps B and C.
Comparative example 5
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 6, except that: the step B1 is as follows: 1 kg of water is mixed with 10 g of bacillus subtilis, 20 g of bacillus thuringiensis and 20 g of trichoderma harzianum to prepare a compound microorganism solution; and astragalus solution is not used in steps B and C.
Comparative example 6
The present comparative example provides a method for inhibiting citrus yellow dragon disease in citrus cultivation, and the specific steps are basically the same as those of example 6, except that: the step B1 is as follows: 1 kg of water is mixed with 5 g of bacillus subtilis, 20 g of bacillus licheniformis and 20 g of trichoderma harzianum to prepare a compound microorganism solution; and astragalus solution is not used in steps B and C.
And (3) effect verification:
1. after the citrus fruits were cultivated for 3 to 4 months in examples 1 to 6 and comparative examples 1 to 6, the cultivated citrus fruits were inspected and compared in terms of the degree of gloss development of citrus leaves, the taste of citrus fruits, the yield of citrus fruits, etc. The specific detection method comprises the following steps:
1) Degree of gloss development of leaf
The method comprises the following steps: observing the gloss development of the leaf
Evaluation criteria for gloss effect: the brighter the blade, the better the stretching.
2) Citrus taste test
The method comprises the following steps: subjective evaluation of comparative tasting sour sweetness.
Evaluation criteria: the higher the sweetness, the better, and the moderate acidity
3) Average citrus yield statistics
The total weight of each citrus plant is directly weighed after citrus harvesting.
The test results of each of the example groups and the comparative example group are shown in table 1.
TABLE 1
2. Prevention and treatment effect of citrus yellow dragon disease
1) The results of comparing the control effects of yellow dragon disease with those of 1-2 annual citrus planted in the high-incidence area (average incidence rate is 2-5%) by the same treatment method of examples 1-6 and comparative examples 1-6 are shown in Table 2.
TABLE 2
2) Citrus having developed yellow dragon disease was treated by the methods of examples 1 to 6 and comparative examples 1 to 6, and the cure rate results of yellow dragon disease are shown in table 3.
TABLE 3 Table 3
There are many ways in which the invention may be practiced, and what has been described above is merely a preferred embodiment of the invention. It should be noted that the above examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that modifications may be made without departing from the principles of the invention, and such modifications are intended to be within the scope of the invention.
Claims (5)
1. The method for inhibiting citrus yellow dragon disease in citrus cultivation is characterized by comprising the following steps of:
A. filling potassium permanganate solution into soil for citrus root growth;
B. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract;
C. after 4-10 days, the soil at the root of the citrus is filled with the compound microorganism solution and the astragalus extract, and the compound microorganism solution is used for spraying the citrus on the whole tree; the treatment times of the step C are 1-3 times;
D. after 4-10 days, spraying the citrus full tree with the composite microbial solution; the treatment times of the step D are 1-8 times;
in steps B, C and D, the compound microorganism solution comprises bacillus subtilis, bacillus licheniformis and trichoderma harzianum; the effective content of the bacillus subtilis is 200-1000 hundred million/g, the effective content of the bacillus licheniformis is 200-1000 hundred million/g, and the effective content of the trichoderma harzianum is 5-50 hundred million/g;
the mass ratio of the bacillus subtilis to the bacillus licheniformis to the trichoderma harzianum is 2-10:1-5:1-5;
in the steps B and C, the mass percentage of the compound microorganism in the compound microorganism solution is 0.01-0.05%;
in the step B, the preparation method of the astragalus extract comprises the following steps: soaking sliced astragalus root in water, boiling for 2-3 hours, and filtering to obtain astragalus root extract;
the mass ratio of the water to the astragalus is 100:1-10.
2. The method for inhibiting citrus yellow long disease in citrus cultivation according to claim 1, wherein the concentration of the potassium permanganate solution is 0.1-0.3%; the filling amount of the potassium permanganate solution in the step A is 15-35 jin/strain;
the time of filling the potassium permanganate solution into the soil for growing the citrus root is 1-3 times; the time interval is 3-5 days each time.
3. The method for inhibiting citrus yellow long disease in citrus cultivation according to claim 1, wherein in each step, the method for filling the potassium permanganate solution, the compound microorganism solution and the astragalus extract is as follows: and taking the root of the trunk as a central point, selecting 2-16 evenly distributed points in the circumference with the diameter of 25-100% of the width of the crown, and irrigating the root of the corresponding potassium permanganate solution, the compound microorganism solution and the astragalus root extracting solution by inserting a root irrigating gun into each point, wherein the inserting depth of the root irrigating gun is 10-50 cm away from the ground.
4. The method for inhibiting citrus yellow long disease in citrus cultivation according to claim 1, wherein the amount of the compound microorganism solution poured in the step B is 2-20 jin/plant; the filling amount of the compound microorganism solution in the step C is 2-20 jin/strain; and C and D, wherein the spraying amount of the compound microorganism solution is 2-20 jin/strain.
5. The method for inhibiting citrus yellow long disease in citrus cultivation according to claim 1, wherein in the steps B and C, the amount of the astragalus extract is 12-32 jin/plant.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255351A (en) * | 2014-10-21 | 2015-01-07 | 中山市巴斯德农业科技有限公司 | Greening disease prevention and control method |
| CN105505823A (en) * | 2015-12-31 | 2016-04-20 | 北京沃土天地生物科技有限公司 | Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer |
| CN105900747A (en) * | 2016-04-21 | 2016-08-31 | 厦门人天景农业科技有限公司 | Method for treating citrus yellow shoot |
| CN106106558A (en) * | 2016-06-25 | 2016-11-16 | 仇颖超 | A kind of preparation method of plant growth promoter |
| CN106857141A (en) * | 2017-01-22 | 2017-06-20 | 邱德文 | A kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen |
| CN107089867A (en) * | 2017-06-01 | 2017-08-25 | 陕西省蒲城美尔果农化有限责任公司 | A kind of somatotrophic wheat dedicated liquid microbial manure of anti-compaction |
| CN108064891A (en) * | 2017-12-01 | 2018-05-25 | 钟山县德福农产品有限公司 | The pesticide control of Citrus Huanglongbing pathogen |
| CN108934714A (en) * | 2018-07-31 | 2018-12-07 | 和平县维康农业科技有限公司 | A kind of citrus yellow shoot disease preventing control method |
-
2020
- 2020-11-20 CN CN202011309952.3A patent/CN112602511B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255351A (en) * | 2014-10-21 | 2015-01-07 | 中山市巴斯德农业科技有限公司 | Greening disease prevention and control method |
| CN105505823A (en) * | 2015-12-31 | 2016-04-20 | 北京沃土天地生物科技有限公司 | Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer |
| CN105900747A (en) * | 2016-04-21 | 2016-08-31 | 厦门人天景农业科技有限公司 | Method for treating citrus yellow shoot |
| CN106106558A (en) * | 2016-06-25 | 2016-11-16 | 仇颖超 | A kind of preparation method of plant growth promoter |
| CN106857141A (en) * | 2017-01-22 | 2017-06-20 | 邱德文 | A kind of method and reagent set for preventing and treating Citrus Huanglongbing pathogen |
| CN107089867A (en) * | 2017-06-01 | 2017-08-25 | 陕西省蒲城美尔果农化有限责任公司 | A kind of somatotrophic wheat dedicated liquid microbial manure of anti-compaction |
| CN108064891A (en) * | 2017-12-01 | 2018-05-25 | 钟山县德福农产品有限公司 | The pesticide control of Citrus Huanglongbing pathogen |
| CN108934714A (en) * | 2018-07-31 | 2018-12-07 | 和平县维康农业科技有限公司 | A kind of citrus yellow shoot disease preventing control method |
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