CN106857141B

CN106857141B - Method and reagent set for preventing and treating citrus yellow shoot

Info

Publication number: CN106857141B
Application number: CN201710046437.2A
Authority: CN
Inventors: 邱德文
Original assignee: Individual
Current assignee: Xiangtan Zhongweike Bioengineering Co ltd
Priority date: 2017-01-22
Filing date: 2017-01-22
Publication date: 2020-06-16
Anticipated expiration: 2037-01-22
Also published as: CN106857141A

Abstract

The invention provides a method for preventing and treating citrus greening disease and a reagent set, belonging to the field of agricultural plant planting, and adopting the technical scheme that the method for preventing and treating the citrus greening disease comprises the following steps; A. applying quicklime to the soil of the citrus orchard according to the usage amount of 50-150 kg/mu; B. applying organic fertilizer containing microbial bacteria to the soil of the citrus orchard according to the using amount of 100-250 kg/mu; C. the immune inducer is sprayed or irrigated to the citrus plants once every 30-40 days, the using amount is 100-150 g/mu, and the immune inducer is continuously used for 3-5 times. The soil acidity is adjusted by quicklime, the organic biological bacterial fertilizer is used in a matching manner, the soil structure and quality are improved, the biological bacteria and the plant immunity inducer are used for promoting the root activity of citrus plants and improving the disease resistance of citrus, so that the citrus yellow shoot is efficiently and greenly prevented and treated, and the healthy growth of the citrus plants is promoted.

Description

Method and reagent set for preventing and treating citrus yellow shoot

Technical Field

The invention belongs to the field of agricultural plant planting, and particularly relates to a method and a reagent set for preventing and treating citrus greening disease.

Background

Huanglongbing is a widespread and extremely harmful citrus disease that occurs widely in over 40 countries and regions, mainly in asia, oceania, africa, and america. Yellow shoot is a serious outbreak in the united states, florida, the major base of citrus production, and more than about 50% of citrus trees are infected with citrus yellow shoot. The xanthomonas sobria pathogen is transmitted by Diaphorinacitri citri, and the pathogenic bacteria are mainly present in the phloem of citrus trees, and as a result, the symptoms of xanthorrhoea sclerosis, mottle and yellowing of citrus leaves, sometimes referred to as zinc or magnesium deficiency, occur. Typical symptoms of affected plants include yellow vein sclerosis, plaques and spots, yellow leaves and small fruits, black roots and rotten fruits cannot ripen, the fruits are bitter and acidic, the economic value is greatly reduced, and huge economic losses are caused to fruit growers. At present, cutting off the yellow shoot sick tree, preventing the diaphorina citri by chemical pesticides and culturing non-toxic seedlings are main control measures for controlling the occurrence of the yellow shoot of the citrus, the loss of the yellow shoot to citrus farmers is up to ten million in the world, the citrus industry is greatly threatened, and strong attention of governments of various countries in the world is attracted.

In China, the yellow shoot disease is getting more serious day by day, the cause is the serious, soil acidification caused by the large amount of quick-acting nitrogen fertilizer in a citrus orchard is one of the main causes which cause the explosion force of the yellow shoot disease year by year, data survey shows that most of citrus varieties can grow better under the condition of pH5-7, the using amount of nitrogen fertilizer in the citrus orchard is about 525 kilograms per hectare in recent years and increases year by year, compared with the using amount which is increased by one time before 30 years, the excessive use of the nitrogen fertilizer can cause soil acidification in the citrus orchard, the pH of the soil in the citrus orchard in Guangxi, Jiangxi and Hunan is 4.5-5.5, and the growth environment of the citrus trees is greatly influenced; on the other hand, studies have shown that citrus trees have an optimal pH for nitrogen, iron, zinc, and B uptake of 6. Under acidic conditions (pH below 5.0), B, Zn and Mg elements cannot be effectively absorbed, and the citrus leaves are yellowed due to the fact that the citrus cannot effectively absorb required elements, root system activity is reduced, plant development is poor, absorption of nutrient substances in soil is reduced, and citrus greening disease is often caused simultaneously with deficiency disease. The citrus huanglongbing disease is getting more serious day by day due to the reasons, the loss caused by the citrus huanglongbing disease is also increased rapidly, and 30 percent of citrus trees in an orchard are infected by the citrus huanglongbing disease in the west and the west of China and are required to be cut off. In Hunan China, the yellow dragon disease causes great loss of 30-50%.

At present, the main methods for preventing or controlling citrus huanglongbing are tree cutting, psyllid killing and non-toxic seedling, but the measures have a plurality of disadvantages, on one hand, the tree cutting causes huge economic loss to fruit growers, and cannot effectively eliminate the huanglongbing and pathogenic bacteria, and secondary pollution also exists; a plurality of chemical pesticides are used in the plant for killing the psyllids, so that the environment, plants and fruits are badly affected, food safety is seriously affected, the affected fruit plants have little effect, and the economic loss is still large; nontoxic seedlings are popular in citrus industry in recent years, but nontoxic seedlings are not disease-resistant, only mean that the seedlings are nontoxic and disease-free when being outplanted, the requirement on the cultivation environment is high, and the infection of yellow shoot can not be effectively prevented. With the deep understanding of the pathogenesis of the yellow dragon disease, the adjustment of the pH value of the soil by using the quicklime is a traditional method for improving the soil acidification. CN 104255351B discloses a method for preventing and treating citrus greening disease, which uses quick lime to disinfect and adjust the pH value of soil to 5.5-7.5, and uses organic water-soluble fertilizer containing chitin and rare earth, mixed liquid of foliar fertilizer containing chitin and rare earth elements and organic silicon and biological fertilizer containing bacillus to treat citrus greening disease, wherein the related fertilizers are many, the steps are fussy, only half of diseased trees recover health after treatment in one year, the treatment effect is poor, the economic loss of investment and recovery is almost the same, and the method is not suitable for large-area popularization.

Therefore, the development of a new method and a product with good effect for preventing and treating citrus greening disease is an urgent technical problem to be solved in the citrus industry.

Disclosure of Invention

In order to solve the technical problems of lack, complex operation and low effect of the existing method for preventing and treating citrus greening disease, the invention provides a method for preventing and treating citrus greening disease and a reagent set.

In order to solve the technical problems, the invention adopts the following technical scheme:

a method of controlling citrus greening disease, said method comprising the steps of;

A. applying quicklime to the soil of the citrus orchard according to the usage amount of 50-150 kg/mu;

B. applying organic fertilizer containing microorganisms to the soil of the citrus orchard according to the using amount of 100-;

C. the immune inducer is sprayed or irrigated to the citrus plants once every 30-40 days, the using amount is 100-150 g/mu, and the immune inducer is continuously used for 3-5 times.

Preferably, the quicklime is applied to the soil of the citrus orchard in the step A, the pH value of the soil is increased by 0.5-1.0 by applying once, and the pH value of the soil is increased to 6.0-6.5 by applying twice finally.

Preferably, step B is directed to citrus fruitWhen organic fertilizer containing microorganism is applied to garden soil, the microorganism content is 0.5-1.5 × 10⁹cfu/mu is added into the organic fertilizer and evenly stirred.

Preferably, the microorganisms comprise trichoderma and bacillus laterosporus, and the number ratio of the trichoderma to the bacillus laterosporus is 1: 1-3.

Preferably, the plant immunity inducer is wettable powder with 3wt.% of active ingredient alternaria tenuissima activator protein content.

The invention also provides a reagent kit for preventing and treating citrus greening disease, which comprises the following reagents in parts by weight, wherein the first reagent is lime, the second reagent is an organic fertilizer containing bacillus, and the third reagent is a plant immunity inducer, wherein the mass part ratio of the first reagent to the second reagent to the third reagent is 5-40: 10-25: 0.03-0.1.

Preferably, the content of the microorganisms in the organic fertilizer containing the microorganisms is 0.5-1.5 x 10⁹cfu/100-250kg。

Preferably, the microorganism comprises trichoderma and bacillus laterosporus.

More preferably, the microorganism consists of the microorganisms in a number ratio of 1: 1-3 of trichoderma and bacillus laterosporus.

In the technical scheme, the method for preventing and treating citrus greening disease and the matched reagent set are provided, wherein the reagent set comprises the following reagents in parts by weight, the first reagent is lime, the second reagent is an organic fertilizer containing microorganisms, the third reagent is a plant immunity inducer, and the mass parts ratio of the first reagent to the second reagent to the third reagent is 5-40: 10-25: 0.03-0.1. When the reagent set is applied to preventing and treating the yellow shoot disease, firstly, quicklime is applied to soil of a citrus orchard according to the usage amount of 50-150 kg/mu, the acidification condition of the soil can be improved by applying the quicklime, the pH value of the soil is increased from below 5 to 6.0-6.5, the activity of a root system can be improved, the effective absorption of elements B, Zn and Mg and nutrient substances in the soil is promoted, a foundation is provided for the establishment of a plant immune system, and the conversion of citrus leaves from yellow to green is promoted, so that the symptoms of a yellow shoot plant are improved and relieved. Furthermore, organic fertilizer containing microorganisms is applied to the soil of the citrus orchard according to the usage amount of 100-250 kg/mu, and the hardened structure of the soil and the organic matter and fertility of the soil can be improved by applying the organic fertilizer containing the microorganisms, so that the growth environment of plant root systems is improved, the root system activity of plants is greatly improved, and on the other hand, the balance of soil floras can be adjusted, the growth of harmful floras is inhibited, and the growth of the plants is promoted. The plant immune inducer is sprayed or irrigated to the citrus plants once every 30-40 days, the using amount is 150 g/mu, the plant immune inducer is continuously used for 3-5 times, the plant immune inducer can promote the plants to generate substances such as salicylic acid, jasmonic acid, ethylene and the like, the synthesis of antibacterial and antiviral substances such as plant protection essence, disease-resistant related protein, phenolic organic matters and the like is further promoted, the defense capability of a plant immune system is greatly improved, the system resistance is obtained, the growth and metabolism of the plants can be promoted, the chlorophyll content of the plants is improved, and the effect of improving the immunity of the plants against various diseases is achieved. The three measures are carried out synchronously, the effects are enhanced by matching, the soil acidification degree is improved through quicklime, the root system activity of the plant is improved, the organic fertilizer containing microorganisms and the plant immunity inducer are applied in a matching manner, a fertile soil foundation is formed while harmful floras are inhibited, the growth of the plant is promoted by further absorbing trace elements and nutrient substances through a developed root system, the chlorophyll increase of the plant is promoted, the defense capability of a plant immune system is greatly improved under the action of the plant immunity inducer by the absorbed trace elements and nutrient substances, the disease resistance of the plant is enhanced, the three measures supplement each other, the prevention and treatment are combined, and both principal and secondary aspects of disease are treated, so that the infected plant is rapidly recovered to be normal, and the plant with high infection risk is prevented from being infected.

The invention has the beneficial effects that: (1) the method and reagent set provided by the invention greatly improve the soil acidification degree, regulate the soil flora, improve the root activity and the immunocompetence of the plants, treat both symptoms and root causes, enable the infected plants to efficiently and quickly recover to normal plants with high infection risk to avoid infection, improve the cure rate of the infected plants and reduce the morbidity of the yellow dragon disease; (2) the method is easy to operate, has simple steps, has no pollution to the environment, plants and fruits, is green and organic, effectively ensures the product quality and quality, and the food safety and the environmental safety, and is suitable for large-scale popularization and use.

Drawings

FIG. 1 is a graph of data of root activity measured in a blank control group and each experimental group fifteen days after a plant immune induction agent is sprayed for the first time in example 2;

FIG. 2A is a graph of the growth of citrus plants infected with Huanglongbing (4 months and 10 days 2016);

FIG. 2B is a graph of the growth of a citrus plant infected with yellow shoot in FIG. 2A after 15 days of its second spraying with a plant immunity inducing agent, treated by a method provided herein;

FIG. 2C is a graph of the growth of a citrus plant infected with yellow shoot in FIG. 2A after treatment with the method of the present invention and spraying of a plant immune inducing agent for the third time, for 15 days.

Detailed Description

The invention provides a method and a reagent set for preventing and treating citrus greening disease, which are described in detail by specific examples below so as to better understand the invention, wherein the related reagents can be obtained from commercial sources without special descriptions, and the related operation steps are conventional operations without special descriptions.

Example 1

The invention provides a method for preventing and treating citrus greening disease and a reagent set, wherein the reagent set comprises the following reagents in parts by weight, wherein the first reagent is quicklime, the second reagent is an organic fertilizer containing microorganisms, and the third reagent is a plant immunity inducer.

Specifically, the mass part ratio of the reagent, namely the quicklime, the reagent, namely the organic fertilizer containing microorganisms, to the reagent, namely the third plant immunity inducer is 5-40: 10-25: 0.03-0.1. Wherein, the organic fertilizer can be chicken manure, cattle and sheep horse manure or bean pulp, cottonseed meal and the like, and the organic matters in the composition are generally controlled to be 35-45wt.%, 30-40 wt.% of water, 20-30 wt.% of inorganic trace elements such as nitrogen, phosphorus, potassium and the like.

The organic fertilizer containing microorganism has microorganism content of 0.5-1.5 × 10⁹The cfu/100 plus 250kg, a proper amount of microorganism can improve the soil structure and adjust the balance of soil flora, inhibit harmful flora and promote the absorption and utilization rate of plant root systems on trace elements and nutrient substances.

The microorganisms comprise trichoderma and bacillus laterosporus, and the quantity ratio of the trichoderma to the bacillus laterosporus is 1: 1-3. The trichoderma can generate various bioactive substances with antagonistic action on plant pathogenic fungi, bacteria and insects, inhibit, weaken and eliminate gray mold pathogens in the same growth environment, improve the stress resistance of crops, induce host plants to generate a self-defense system to obtain disease resistance, increase the activity of roots and seedlings, promote the growth of plants and improve the yield of agricultural products; the bacillus laterosporus secretes a large amount of chitinase, inhibits fungal diseases, simultaneously decomposes organic matters and promotes the absorption and utilization rate of the fertilizer, the bacillus laterosporus and the bacillus laterosporus are scientifically and reasonably proportioned, simultaneously the soil improvement effect and the harmful flora inhibiting effect are ensured, the absorption and utilization rate of the fertilizer is also promoted, and the bacillus laterosporus has a promotion effect on the establishment of a plant autoimmune system.

The plant immunity inducer is wettable powder with the active ingredient of 3wt.% of alternaria tenuissima activator protein. The alternaria tenuissima activator protein is a bioactive, single and stable protein extracted from microbial fermentation, can stimulate the increase of the enzymatic activity of a plant body, thereby stimulating the regulation and control of the metabolism of the plant body, stimulating the growth of roots, stems and leaves of the plant body, and improving the chlorophyll content, and has the following functions: (1) the growth of the plant root system is promoted, and the absorption capacity of the root system is enhanced, so that the crop yield is improved; (2) pathogenic bacteria breeding inside and outside the plant body is controlled, and plant diseases and insect pests are reduced; (3) the metabolism of plants is enhanced, the photosynthesis is promoted, the protective film of leaves is strengthened, and pathogenic bacteria are resisted; (4) the photosynthesis is enhanced, and the utilization rate of the fertilizer is improved; on the other hand, the protein is good in stability, easy to dissolve in water, stable in pH 4-10, resistant to high temperature, stable after being boiled in 100 ℃ boiling water for 30min, free of influence on the effect in natural light for 2d, stable after being stored for 2 years at normal temperature, more guaranteed in effect stability and convenient to store, and is in the form of wettable powder, wherein the content of the active ingredient, namely the Alternaria alternata activator protein, is 3 wt.%.

The reagent set is scientifically and reasonably prepared with the amounts and the proportions of the three reagents, quicklime is used for adjusting the soil acidity and improving the plant root activity, and organic fertilizers containing microorganisms are used in a matched manner, so that the soil structure and the quality are improved, the flora balance is also adjusted, harmful flora is inhibited, the biological bacteria are further used for being matched with a plant immunity inducer to promote the root activity of citrus plants and improve the disease resistance of an immune system of citrus trees, and therefore, the citrus yellow shoot is efficiently and greenly prevented and the healthy growth of the citrus plants is promoted.

Example 2 this example demonstrates the efficacy of the method to control citrus greening disease through detailed field experiments

The method and the reagent set of the invention are used for field contrast test for preventing and controlling citrus yellow shoot

Orchards with similar incidence degree and growth condition of huanglongbing are selected to be respectively used as 4 experimental groups (the labels are A, B, C and D respectively) and 1 blank control group (the label is CK), each group treats 30 fruit trees, and the five groups are treated as follows simultaneously:

and (3) CK group: conventional management without special treatment;

group A: applying quicklime to the soil of the citrus orchard according to the usage amount of 50-150 kg/mu, wherein the quicklime is required to be uniformly scattered to cover the ground surface of the whole treatment area;

group B: applying organic fertilizer containing microorganisms to the soil of the citrus orchard according to the using amount of 100-. The operation method is that a straight ditch or an annular ditch is dug deeply along the position (water dripping line) of the crown of the fruit tree row, the ditching depth is required to be 30cm, the width is required to be 20-30cm, organic fertilizer containing microorganisms is uniformly applied in the ditch, and the fertilizer application is required to be ensured to be uniform;

group C: spraying or irrigating the immune inducer to the citrus plants according to the using amount of 100-150 g/mu, continuously spraying or irrigating the roots once again every 30-40 days, and continuously using for 3 times, wherein the requirement of uniform spraying on the leaf surfaces until water drops drop;

group D: the quick lime is applied to the soil of a citrus orchard according to the usage amount of 50-150 kg/mu of quick lime and 100-250 kg/mu of organic fertilizer containing microorganisms, meanwhile, the plant immune inducer is sprayed or irrigated to the citrus plants according to the usage amount of 100-150 g/mu, and then the plant immune inducer is sprayed or irrigated once every 30-40 days for 3 times of continuous use, which is required to be the same as the components.

After treatment, the disease incidence of the yellow dragon disease of the fruit trees in each treatment group is investigated respectively 15 days after the spraying of the plant immunity inducer for the 1 st time, 15 days after the spraying of the plant immunity inducer for the 2 nd time and 15 days after the spraying of the plant immunity inducer for the 3 rd time, and the statistics is carried out according to the disease indexes and the control effects of each group of 2.1, and the results are shown in table 1; 6 plants were randomly selected from each group 15 days after the 3 rd spraying to measure root activity according to the following 2.2TTC method.

2.1 disease index and prevention and treatment effects of each group

(1) The classification standard of the citrus greening disease is as follows:

level 0: the whole tree is disease-free;

level 1: 1-2 shoots on the fruit tree have mottled etiolated leaves;

and 3, level: part of lateral branches or main branches have mottled yellowing symptoms, and symptom branches account for less than 1/3 of the whole tree;

and 5, stage: the symptom lateral branches or main branches account for more than 1/3 and less than 2/3 of the whole tree;

and 7, stage: the symptom branches are above 2/3;

and 9, stage: the whole tree died.

(2) Calculation method

1) Disease index = [ ∑ (number of disease strains at each stage × relative grade value)/(total number of investigated strains × 9) ] × 100

2) Control effect (%) = (CK 1-PT 1)/CK 1 × 100

Note: CK1 represents disease index of placebo; PT1 represents disease index for each treatment group.

According to the grading standard and the calculation method, survey data and prevention and treatment effect data are counted, disease grading and disease index calculation are carried out on initial symptoms before spraying, the initial states of 5 groups are 12 plants at the 0 level, 9 plants at the 1 level, 6 plants at the 3 level, 2 plants at the 5 level, 1 plant at the 7 level and 0 plants at the 9 level, the disease index is 16.3, and the results are shown in the following table 1.

TABLE 1 data of the prevention and treatment effects of Huanglongbing at different treatment periods

As can be seen from the test data, the A, B, C, D groups of fruit trees treated by the method all have different degrees of reduction compared with the yellow dragon disease of the blank control group. 15 days after the 1 st spraying, the control effects of A, B, C, D groups are respectively as follows: 4.26 percent, 4.26 percent and 23.40 percent, only the group D treated in a combined way has obvious control effect which is greatly higher than the sum of three measures used independently, the reason is that the group A only improves the pH value of soil, the root activity is not recovered, in addition, the soil is barren and the nutrition is deficient, the control effect is not obvious, after the group B is applied with organic fertilizer containing microorganisms, the soil fertility is enhanced, but the soil acidity is still very large, the absorption is not facilitated, the disease resistance of plants is not strong, the group C sprays plant immunity inducer, the immunity is improved, healthy plants are not infected continuously, but the soil acidity is large, the fertility is weak, the recovery force of infected plants is weak, the control effect is not obvious, only the group D treated in a combined way increases the soil fertility, inhibits harmful flora, improves the soil acidity and the flora balance, greatly improves the root activity, is favorable for absorbing nutrient substances and improving the immunity to promote the plant growth, meanwhile, the plant immunity inducer also greatly improves the disease resistance of plants, so that infected plants are well transformed, healthy plants are prevented from being infected, the three measures are mutually coordinated, and the prevention and treatment effect is greatly enhanced. 15 days after the 2 nd spraying, the control effects of the A, B, C, D groups are respectively as follows: 10.61%, 18.18%, 25.76% and 59.09%, along with the extension of the treatment time, the control effects of A, B and C groups which are independently used by the measures are separated from each other, so that the plant immunity inducer is applied to improve the disease resistance of the plant autoimmune system, the control effect is better, the fertilizer is applied, the B group with balanced soil flora is improved for the second time, the control effect of the A group with the balanced soil flora is the worst, the control effect of the D group with the three measures used in a combined manner is greatly higher than the control effects of A, B and C groups which are independently used by the three measures respectively, and is about 15% higher than the sum of the control effects of the three groups; 15 days after the 3 rd spraying, the control effects of the A, B, C, D groups are respectively as follows: 9.09 percent, 23.86 percent, 30.86 percent and 78.41 percent, and after the treatment of one period, the final prevention and treatment effect of the huanglongbing reaches 78.41 percent to the maximum after the comprehensive treatment of the three treatment modes, and is about 25 percent higher than the sum of the prevention and treatment effects of A, B and the group C.

Method for measuring root activity

(1) Preparation of TTC (triphenyltetrazolium chloride) standard curve

(1.1) preparation of a solution

Preparation of 0.4% TTC solution: 0.4g of TTC (triphenyltetrazolium chloride) is weighed and dissolved in 100mL of distilled water and is stirred uniformly;

(1.2) preparation of a calibration Curve

0.25ml of 0.4% TTC solution is measured and transferred to a 10ml volumetric flask, a little sodium thiosulfate powder is added, red TTF (triphenylmethane) is generated immediately after shaking up, then ethyl acetate (analytically pure) is added to the volume to be the scale mark, and the solution is shaken up to obtain 0.1% TTF solution. 0.25mL, 0.5mL, 1.00mL, 1.50mL and 2.00mL of 0.1% TTF solution are respectively measured and added into 5 10mL volumetric flasks, ethyl acetate is respectively used for constant volume to scale marks, the standard colorimetric series respectively containing 25 mug, 50 mug, 100 mug, 150 mug and 200 mug of TTF (the concentration is respectively 2.5 mug/mL, 5 mug/mL, 10 mug/mL, 15 mug/mL and 20 mug/mL) are obtained by shaking up, the TTF solution is used as a blank control, the absorbance is measured at 485nm, and a TTF absorbance standard curve is drawn.

(2) Determination of plant root vigor

(2.1) Experimental group and blank control group for setting plant roots

Experimental groups: weighing 0.5g of root sample, putting the root sample into a culture dish, adding 10ml of solution mixed by 0.4% TTC solution and phosphate buffer (1/15 mu mol/L, pH value is 7) in equal amount, fully immersing the root in the solution, preserving the temperature for 1-3h in a dark place at 37 ℃, and then adding 2ml of 1mol/L sulfuric acid to stop the reaction;

blank control group: different from the experimental group, 2ml of 1mol/L sulfuric acid is added before the root sample is added, and other operations are the same;

(2.2) taking out the roots, sucking water, and grinding with 3-4 ml of ethyl acetate and a small amount of quartz sand to extract red TTF. Collecting the red extract in a test tube, washing the root residue with a small amount of ethyl acetate for two to three times, transferring into the test tube, adding ethyl acetate to make the total amount to be 10ml, measuring the light absorption of the extract at 485nm by a spectrophotometer, reading the absorbance by taking a blank as a reference, obtaining the reduction amount of tetrazole by a standard curve, and substituting the obtained data into the following formula a to obtain the reduction intensity of tetrazole.

Formula a, the reduction strength of tetrazole (mg/g (fresh root weight)/h) is tetrazole reduction amount (mg)/[ root weight (g) × time (h) ].

Finally, the average values of all the groups are used as result data to prepare a data table, as shown in figure 1, it can be seen that the root system activity of the group D comprehensively treated by the method is greatly enhanced, on one hand, the activity is beneficial to improving the soil acidity by quicklime, on the other hand, the plant immunity inducer enables the plant to establish a strong immune system, and the promotion effect of microorganisms and organic fertilizers, the three measures are mutually matched, the virtuous cycle is mutually enhanced, the root system of the plant is greatly enhanced, and the plant is enabled to grow healthily.

In conclusion, the three measures of the method for preventing and treating the citrus greening disease provided by the invention coordinate to greatly improve the disease condition of the infected plants in the plants of the orchard with the greening disease, prevent the plants which are not infected with the greening disease from being infected with the greening disease, and greatly improve the prevention and treatment effect of the greening disease in the orchard.

Claims

1. A method of controlling citrus greening disease, said method comprising the steps of:

C. spraying or irrigating the immune inducer to the citrus plants once every 30-40 days, wherein the using amount is 100-; in the step A, quicklime is applied to soil in a citrus orchard, the pH value of the soil is increased by 0.5-1.0 after one application, and the pH value of the soil is increased to 6.0-6.5 after two applications; the microorganisms comprise trichoderma and bacillus laterosporus, and the quantity ratio of the trichoderma to the bacillus laterosporus is 1: 1-3; the plant immunity inducer is wettable powder with the active ingredient of 3wt.% of alternaria tenuissima activator protein.

2. The method for controlling citrus greening disease according to claim 1, wherein said step B is carried out by applying organic fertilizer containing microorganisms to soil in citrus orchard in an amount of 0.5 to 1.5 x 10 in terms of microorganisms⁹cfu/mu is added into the organic fertilizer and evenly stirred.

3. The reagent set for preventing and treating citrus greening disease is characterized by comprising the following reagents, wherein the reagent I is quicklime, the reagent II is an organic fertilizer containing microorganisms, and the reagent III is a plant immunity inducer, wherein the mass part ratio of the reagent I to the reagent II to the reagent III is 5-40: 10-25: 0.03-0.1; the microorganism comprises trichoderma and bacillus laterosporus; the plant immunity inducer is wettable powder with the active ingredient of 3wt.% of alternaria tenuissima activator protein.

4. The kit for controlling citrus greening disease according to claim 3, wherein the content of microorganisms in the organic fertilizer containing microorganisms is 0.5-1.5 x 10⁹cfu/100-250kg。

5. The kit for controlling citrus greening disease according to claim 3, wherein said microorganisms are selected from the group consisting of microorganisms having a number ratio of 1: 1-3 of trichoderma and bacillus laterosporus.