CN103238633B - Prevention solution for preharvest disease of Huping jujube and application thereof - Google Patents
Prevention solution for preharvest disease of Huping jujube and application thereof Download PDFInfo
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- CN103238633B CN103238633B CN201310183467.XA CN201310183467A CN103238633B CN 103238633 B CN103238633 B CN 103238633B CN 201310183467 A CN201310183467 A CN 201310183467A CN 103238633 B CN103238633 B CN 103238633B
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 55
- 201000010099 disease Diseases 0.000 title claims abstract description 54
- 230000002265 prevention Effects 0.000 title abstract 5
- 238000012794 pre-harvesting Methods 0.000 title abstract 4
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 134
- 241000222051 Papiliotrema laurentii Species 0.000 claims abstract description 92
- 241001247821 Ziziphus Species 0.000 claims abstract description 29
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000011575 calcium Substances 0.000 claims abstract description 15
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 15
- 239000002366 mineral element Substances 0.000 claims abstract description 9
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims abstract description 8
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052796 boron Inorganic materials 0.000 claims abstract description 8
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 8
- 239000010703 silicon Substances 0.000 claims abstract description 8
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 8
- 239000011701 zinc Substances 0.000 claims abstract description 8
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical group [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 20
- 229960002401 calcium lactate Drugs 0.000 claims description 19
- 239000001527 calcium lactate Substances 0.000 claims description 19
- 235000011086 calcium lactate Nutrition 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 17
- 239000000725 suspension Substances 0.000 claims description 17
- 239000004115 Sodium Silicate Substances 0.000 claims description 16
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 16
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 16
- 239000007921 spray Substances 0.000 claims description 16
- WUUHFRRPHJEEKV-UHFFFAOYSA-N tripotassium borate Chemical compound [K+].[K+].[K+].[O-]B([O-])[O-] WUUHFRRPHJEEKV-UHFFFAOYSA-N 0.000 claims description 16
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 16
- 239000011686 zinc sulphate Substances 0.000 claims description 16
- 235000009529 zinc sulphate Nutrition 0.000 claims description 16
- 229960005069 calcium Drugs 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000000470 constituent Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 239000000575 pesticide Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 229920001661 Chitosan Polymers 0.000 abstract 2
- 230000006806 disease prevention Effects 0.000 abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 13
- 206010039509 Scab Diseases 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 238000005507 spraying Methods 0.000 description 7
- 244000126002 Ziziphus vulgaris Species 0.000 description 6
- 235000008529 Ziziphus vulgaris Nutrition 0.000 description 6
- 230000004345 fruit ripening Effects 0.000 description 6
- 230000001788 irregular Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
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- 239000012138 yeast extract Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
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- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
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- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a prevention solution for preharvest disease of Huping jujube and application thereof and discloses a fruit disease prevention solution. The provided fruit disease prevention solution contains mineral elements, chitosan and cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner), wherein the mineral elements comprises the elements of calcium, boron, zinc and silicon; the ratio of the calcium to the boron to the zinc to the silicon to the chitosan to the cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is (18.3-36.7g):(1.4-7.1g):(2.3-4.5g):(0.99-4.9g):(50-200g):(1*10<10>-1*10<12>CFU); and the calcium comes from organic calcium. According to the prevention solution, the amount of chemical pesticide and residual hazard of the pesticide on the fruits thereof are reduced, the prevention solution has the obvious effect of preventing the preharvest diseases of Huping jujube, and compared with the control group, the preharvest disease rate of Huping jujube can be reduced by 69.9 percent, and the prevention solution is convenient to use.
Description
Technical field
The present invention relates to a kind of fruit disease control liquid, particularly a kind of tall bottle with spout jujube is adopted control liquid and the application thereof of front disease.
Background technology
One of tall bottle with spout Zao Shi China ten your name's jujubes in Shanxi, there is establishing in large scale on the ground such as Shaanxi, Xinjiang, occupy very important status in the agricultural production of local farmers and life.Only the area under cultivation of Shanxi Jinzhong area tall bottle with spout jujube just exceedes 1,000,000 mu, is the column support type industry of local agro based economic development.But in recent years, tall bottle with spout date fruit is adopted front disease and is on the rise, cause large area loss and the underproduction, extremely seriously affect the development of tall bottle with spout jujube industry.Tall bottle with spout date fruit is adopted front disease main manifestations for really dark red scab in irregular shape appears in top or umbilical region, scab is just that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue, go deep into pulp, taste is extremely bitter.Morbidity later stage fruit site of pathological change is organized gradually and is rotted.
At present, orchard worker mainly adopts front disease to tall bottle with spout jujube and prevents and treats by spraying various chemical pesticides, but protection effect is very undesirable.Opinions vary for the cause of disease of this disease, by the system investigation to disease pests occurrence rule and phenotype and research, the microorganism that finds the cause of disease not is the principal element of bringing out this disease, after healthy date fruit, can not cause similar symptom because separate from date fruit the various pathogen tiebacks that obtain, and incidence tissue is directly inoculated in to healthy pulp, fruit is not also fallen ill; It is cotton-shaped that incidence tissue becomes, similar to the physiological disturbance of observing on other fruits.After date fruit morbidity, infecting of pathogenic microorganism is to increase the weight of the main cause that disease occurs.Adopt front disease if will effectively prevent and treat tall bottle with spout jujube, need to consider the various factors that causes this disease.
Summary of the invention
An object of the present invention is to provide a kind of fruit disease control liquid.
Fruit disease control liquid provided by the present invention, contains mineral element, shitosan and Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner); Described mineral element is calcium constituent, boron element, zinc element and element silicon; The proportioning of described calcium constituent, described boron element, described zinc element, described element silicon, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is 18.3～36.7g:1.4～7.1g:2.3～4.5g:0.99～4.9g:50～200g:1 × 10
10～1 × 10
12cFU; Described calcium constituent derives from organic calcium.
In the present invention, described organic calcium is specially calcium lactate; Described boron element derives from potassium borate; Described zinc element derives from zinc sulphate; Described element silicon derives from sodium silicate;
In described control liquid, the proportioning of described calcium lactate, described potassium borate, described zinc sulphate, described sodium silicate, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) can be 100～200g:10～50g:10～20g:10～50g:50～200g:1 × 10
10～1 × 10
12cFU, as 200g:50g:10～20g:20～50g:100～200g:1 × 10
11～1 × 10
12cFU, as 200g:50g:20g:30～50g:100～200g:1 × 10
12cFU, for another example 200g:50g:20g:50g:200g:1 × 10
12cFU.
In described control liquid, also contain the medium of water and the described Cryptococcus laurentii of cultivation (Cryptococcus laurentii (Kuffer.) Skinner).
More concrete, prevent and treat the composed as follows of liquid: 10～20g(as 10g or 20g described in every 1L) as described in calcium lactate, 1～5g(as 1g or 5g) as described in potassium borate, 1～2g(as 1g or 2g) as described in zinc sulphate, 1～5g(as 1g, 2g, 3g or 5g) as described in sodium silicate, 5～20g(as 5g, 10g or 20g) as described in shitosan, 1 × 10
9～1 × 10
11cFU(is as 1 × 10
9cFU, 1 × 10
10cFU or 1 × 10
11cFU) described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner), surplus is that volume ratio is the water of 1:1 and the medium of cultivating described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner).
The solvent of the medium of described cultivation Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is water, and solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.
In the present invention, described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is specially Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029.
Another object of the present invention is to provide the preparation method of described control liquid.
The preparation method of described control liquid, can comprise the steps:
(1) calcium lactate, potassium borate, zinc sulphate, sodium silicate, shitosan and water are mixed according to the ratio of 100～200g:10～50g:10～20g:10～50g:50～200g:5L, preparation obtains solution I;
(2) get and the isopyknic Cryptococcus laurentii of water described in step (1) (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension, mix with the described solution I that step (1) obtains, making Cryptococcus laurentii described in mixed liquor (Cryptococcus laurentii (Kuffer.) Skinner) and the ratio of described calcium lactate is 1 × 10
10～1 × 10
12cFU:100～200g, gained mixed liquor is described control liquid.
In the present invention, the preparation method of described control liquid, specifically comprises the steps:
(a1) by 100～200g(as 100g or 200g) calcium lactate, 10～50g(be as 10g or 50g) potassium borate, 10～20g(be as 10g or 20g) zinc sulphate, 10～50g(is as 10g, 20g, 30g or 50g) sodium silicate, 50～200g(be as 50g, 100g or 200g) shitosan is dissolved in 5L water, obtains solution I;
(a2) getting 5L bacterial content is 2 × 10
6～2 × 10
8cFU/mL(is as 2 × 10
6cFU/mL, 2 × 10
7cFU/mL or 2 × 10
8cFU/mL) the described solution I that Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension and step (a1) obtain is mixed, and obtains described control liquid.
In said method, described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension is for being placed in described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension obtaining after following medium is cultivated: solvent is water, solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.
In said method, described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is specially Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029.
The application in disease before control tall bottle with spout jujube is adopted of described control liquid also belongs to protection scope of the present invention.
Another object of the present invention is to provide a kind of method of preventing and treating tall bottle with spout jujube and adopt front disease.
Control tall bottle with spout jujube provided by the present invention is adopted the method for front disease, is from tall bottle with spout jujube young fruit period or expanding stage, regularly described control liquid is directly sprayed on the fruit and blade of described tall bottle with spout jujube, until before gathering 3 weeks time only.
In said method, it is that 0.5-1.0cm(is as 0.5cm that described young fruit period can be described tall bottle with spout date fruit major diameter) time; Described expanding stage is that described tall bottle with spout date fruit major diameter is that 2-3cm(is as 3cm) time.
In said method, described in the frequency that sprays be 2-4 week/time (as 2 weeks/time or 4 weeks/time).
In said method, described in the dosage that sprays be that every tree sprays 2L at every turn.
In the present invention, described shitosan is that molecular weight is the shitosan of 350kDa.In one embodiment of the invention, described shitosan is specially Qingdao Honghai Biotechnology Co., Ltd.'s product, and its catalog number is: YZ080115.
In the present invention, described disease or described in adopt front disease Disease Characters be: there is dark red scab in fruit top or umbilical region.
More concrete, described Disease Characters is: dark red scab in irregular shape appears in fruit top or umbilical region, and scab is just that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue, gos deep into pulp, and taste is extremely bitter; Morbidity later stage fruit site of pathological change tissue rots.
Fruit disease provided by the present invention control liquid, wherein calcium lactate, potassium borate and zinc sulphate can effectively supplement the date fruit required mineral element that grows; Calcium lactate is organic calcium, plant is not produced to poisoning; Sodium silicate can improve self disease resistance of fruit; Shitosan can form diaphragm at fruit surface as fruit glaze agent, hinders infecting of pathogenic microorganism; And Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) can effectively reduce the superinfection of pathogenic microorganism as biological control saccharomycete, play the effect of integrated control.Experiment showed, that adopting front disease effect with control liquid control tall bottle with spout jujube provided by the present invention significantly (compared with control group, can adopt tall bottle with spout jujube front disease rate and reduce by 69.9%), and easy to use.The present invention is not in the situation that using chemical pesticide; Integrated using mineral element and antagonist; can effectively prevent and treat tall bottle with spout jujube and adopt front disease, reduce the consumption of chemical pesticide and the residual hazard on fruit thereof, play protection health and antipollution effect.
Embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.
Calcium lactate (Xilong Chemical Co., Ltd; Catalog number: 814-80-2), potassium borate (Chemical Reagent Co., Ltd., Sinopharm Group; Catalog number: 12045-78-2), zinc sulphate (Xilong Chemical Co., Ltd; Catalog number: 7446-20-0), sodium silicate (Xilong Chemical Co., Ltd; Catalog number: 13517-24-3).
Shitosan: molecular formula is [C6H13NO5] n, and molecular weight is 350kDa, is Qingdao Honghai Biotechnology Co., Ltd.'s product, and its catalog number is: YZ080115.
Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3 bacterial strain: this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 12nd, 2003 and (is called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), the preservation center numbering of registering on the books: CGMCC No.1029.This bacterial strain has been on November 19th, 2003 in the applying date, in the patent application that application number is 200310115336.4, discloses, and patent publication No. is CN1544614A.
Yeast Cultivation base: solvent is water, and solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.Wherein, yeast extract is that Beijing bispin microorganism is cultivated company's product, and its catalog number is CM02-12A; Peptone Beijing bispin microorganism is cultivated company's product, and its catalog number is CM02-31.
Each test in following embodiment is all carried out in experiment orchard, Qingxu County, Shanxi province Taiyuan city.In test according to following criterion, assert whether fruit is disease fruit: sick fruit shows as: dark red scab in irregular shape appears in fruit top or umbilical region, scab is just that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue, go deep into pulp, taste is extremely bitter; Morbidity later stage fruit site of pathological change is organized gradually and is rotted.
Embodiment 1:
One, the preparation of fruit disease control liquid
1, by Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029, be inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivate 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
6cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 100g, potassium borate 10g, zinc sulphate 10g, sodium silicate 10g, shitosan 50g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
6cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter), every 2 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 51.8%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 33.4%.
Embodiment 2:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
7cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 10g, sodium silicate 20g, shitosan 100g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
7cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter), every 2 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 25.6%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 59.6%.
Embodiment 3:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
8cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 30g, shitosan 100g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
8cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter), every 2 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 17.8%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 67.4%.
Embodiment 4:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
8cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
8cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter), every 2 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 15.3%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 69.9%.
Embodiment 5:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
8cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
8cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter), every 4 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 49.5%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 35.7%.
Embodiment 6:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hours, obtain seed liquor; With the concentration of Cryptococcus laurentii described in the whole described seed liquor of described Yeast Cultivation keynote (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 be 2 × 10
8cFU/mL, obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to completely and dissolve, is then 2 × 10 with 5L bacterial strain concentration
8cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 bacteria suspension of CFU/mL mixes, and stirs, and obtains fruit disease control liquid.
Two, tall bottle with spout jujube is adopted the control of front disease
From tall bottle with spout date fruit expanding stage (3 centimetres of fruit major diameters), every 2 weeks, the control liquid of step 1 preparation is directly sprayed on tall bottle with spout date fruit and blade, until before gathering 3 weeks time only, sprayed dose is that every strain is set each 2 liters and prevented and treated liquid (experimental group).Every processing 10 strain jujube trees, to spray clear water in contrast.Before fruit maturation is plucked, the tall bottle with spout date fruit of statistical experiment group and control group is adopted front diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity × 100%.
Result shows, spraying the experimental group tall bottle with spout date fruit of control liquid, to adopt front diseased fruit rate be 56.7%, and the control group diseased fruit rate that sprays clear water is 85.2%, adds up to experimental group to adopt front diseased fruit rate compared with control group and has reduced 28.5%.
The result of comprehensive above each embodiment, can find out, use fruit control liquid control tall bottle with spout date fruit provided by the present invention to adopt the effect of front disease, with control liquid Mineral Elements, fruit glaze agent, the saccharomycetic working concentration of biological control, fruit developmental stage when control liquid uses, use interval time etc. relevant, with embodiment 4 best results, with control group phase specific energy, tall bottle with spout date fruit is adopted to front disease rate and reduce by 69.9%.
Claims (6)
1. fruit is adopted a front disease control liquid, contains mineral element, shitosan and Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029; Described mineral element is calcium constituent, boron element, zinc element and element silicon; The proportioning of described calcium constituent, described boron element, described zinc element, described element silicon, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is 18.3～36.7g:1.4～7.1g:2.3～4.5g:0.99～4.9g:50～200g:1 × 10
10～1 × 10
12cFU; Described calcium constituent derives from organic calcium;
Described fruit is tall bottle with spout jujube.
2. control liquid according to claim 1, is characterized in that: described organic calcium is calcium lactate; Described boron element derives from potassium borate; Described zinc element derives from zinc sulphate; Described element silicon derives from sodium silicate;
In described control liquid, the proportioning of described calcium lactate, described potassium borate, described zinc sulphate, described sodium silicate, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is 100～200g:10～50g:10～20g:10～50g:50～200g:1 × 10
10～1 × 10
12cFU.
3. the preparation method who prevents and treats liquid described in claim 2, comprises the steps:
(1) calcium lactate, potassium borate, zinc sulphate, sodium silicate, shitosan and water are mixed according to the ratio of 100～200g:10～50g:10～20g:10～50g:50～200g:5L, preparation obtains solution I;
(2) get and the isopyknic Cryptococcus laurentii of water described in step (1) (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension, mix with the described solution I that step (1) obtains, making the ratio of (Cryptococcus laurentii (Kuffer.) Skinner) the BAY-3CGMCC No.1029 of Cryptococcus laurentii described in mixed liquor and described calcium lactate is 1 × 10
10～1 × 10
12cFU:100～200g, gained mixed liquor is control liquid.
4. the application in disease before control tall bottle with spout jujube is adopted of the control liquid described in claim 1 or 2.
5. prevent and treat tall bottle with spout jujube and adopt the method for front disease for one kind, it is characterized in that: the method is from tall bottle with spout jujube young fruit period or expanding stage, according to 2-4 week/time spray frequency, every strain set the sprayed doses of each 2 liters of control liquid the control liquid described in claim 1 or 2 directly sprayed on the fruit and blade of described tall bottle with spout jujube, until before gathering 3 weeks time.
6. method according to claim 5, is characterized in that: in said method, described young fruit period is that described tall bottle with spout date fruit major diameter is while being 0.5-1.0cm; Described expanding stage is that described tall bottle with spout date fruit major diameter is while being 2.0-3.0cm.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543796A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Method for preventing and treating postharvest disease of fruit and vegetable using sodium silicate or potassium silicate and liquid for preventing and treating postharvest disease of fruit and vegeta |
| CN1544614A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Bacteria for biocontrol of disease and its application |
| CN101491273A (en) * | 2009-03-04 | 2009-07-29 | 中国科学院植物研究所 | Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent |
| CN101914459A (en) * | 2010-04-20 | 2010-12-15 | 浙江大学 | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543796A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Method for preventing and treating postharvest disease of fruit and vegetable using sodium silicate or potassium silicate and liquid for preventing and treating postharvest disease of fruit and vegeta |
| CN1544614A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Bacteria for biocontrol of disease and its application |
| CN101491273A (en) * | 2009-03-04 | 2009-07-29 | 中国科学院植物研究所 | Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent |
| CN101914459A (en) * | 2010-04-20 | 2010-12-15 | 浙江大学 | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii |
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