CN104782675B - Novel plant disease biocide and preparation method thereof - Google Patents
Novel plant disease biocide and preparation method thereof Download PDFInfo
- Publication number
- CN104782675B CN104782675B CN201510116696.9A CN201510116696A CN104782675B CN 104782675 B CN104782675 B CN 104782675B CN 201510116696 A CN201510116696 A CN 201510116696A CN 104782675 B CN104782675 B CN 104782675B
- Authority
- CN
- China
- Prior art keywords
- biocide
- plant disease
- plant
- parts
- ginseng
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
Novel plant disease biocide and preparation method thereof, it is that the active ingredient in the Chinese medicine materials such as pseudo-ginseng, felwort, radix stellariae dichotomae, kuh-seng, rheum officinale, rattletop, dandelion is drawn using bio-fermentation engineering and Biochemical Engineering technology, and by special process and a kind of new more effect plant disease biocides being configured to.The biocide simultaneously effectively anti-can kill the pathogenic microorganisms such as the fungi for causing plant disease, bacterium and virus, but the equal non-hazardous effect of growth to crop seedling and explant in the explant and cell and greenhouse in Plant Tissue Breeding, cell engineering culture.And no matter during production or use, equal contamination-free produces.It is the preferable substitute products of the widely used similar drugs preparation in tissue cultures and cell culture at present.The present invention has effect good, the characteristics of having no toxic side effect.Energy is safe efficient, quick, widely kills the plant as caused by pathogenic microorganisms, Chinese medicine, the plant disease of anti-season fruits and vegetables, is the novel plant disease biocide product that national society's planting industry is badly in need of, and market prospects are boundless.
Description
Technical field
It is special the invention belongs to application bio-fermentation engineering and the modern Chinese herbal medicine manufacturing technology field of Biochemical Engineering technology
It is not related to using Chinese medicine as novel plant disease biocide of raw material and preparation method thereof.
Background technology
Novel plant disease biocide has safe, efficient, fast feature, also has to resist to kill simultaneously and a variety of causes plant
The function of the pathogenic microorganisms such as fungi, bacterium and the virus of thing disease.It can be widely used for resisting and kill Plant Tissue Breeding, cell culture
The plant disease of high Chinese medicine and anti-season fruits and vegetables with greenhouse cultivation economic value.It is better than at present in tissue cultures, cell training
The antibacterial agricultural chemicals and preparation used in foster and greenhouse.And biocidal efficacies are trained also superior to the now widely used plant tissue that is used for
Support, cell culture and U.S. PPM are anti-to kill agent product.
Novel plant disease biocide has following advantage：1. dosage is small；2. to plant explant, callus and thin
The damage of born of the same parents is small；3. noresidue.
The content of the invention
It is an object of the invention to provide a kind of novel plant disease biocide and preparation method.In the hope of being substituted with the biocide
In the market is existing to be used for plant antibiotic, and the pathogenic microorganisms such as the fungi for causing plant disease, bacterium and virus are killed to resist, and
To the crop seedling in the explant in plant cell engineering culture and cell and greenhouse and the equal non-hazardous effect of explant.
The concrete technology preparation process of the present invention is such：
Step 1, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae are respectively put into boiling vessel and soaks, boil,
Afterwards by three kinds gained decoctions it is filtered, sterilizing after by 1: 4: 2 part by weight be well mixed nutrient solution is made, then in nutrient solution
In by specific " volume/weight " proportioning add (NH4)2HPO4、KH2PO4、MgSO47H2O、NaC1、FeSO47H2O, sucrose and grape
Sugar；Nutrient solution is subjected to inoculated and cultured fermentation again, so as to produce the core antibiosis liquid that can kill pathogenic microorganisms, then to the antibiosis
Liquid carries out sterilization treatment and obtains main material extract solution --- " biofermentation antibiosis liquid "；
The fermented bacterium of biofermentation antibiosis liquid is：" aspergillus oryzae Aspergillus oryzae ".
In the inoculated and cultured fermentation operation described in the step, (NH is added per 1000ml nutrient solutions4)2HPO40.5g~1g,
KH2PO40.3g~0.8g, MgSO40.03g~0.08g, NaC10.5g~1.5g, FeSO40.02g~0.05g, sucrose 2g~6g
With glucose 0.5g~1.5g；30 ± 1 DEG C of cultivation temperature, incubation time 7~14 days.
Step 2, by cleaned auxiliary material kuh-seng, rheum officinale, rattletop, dandelion, it is respectively put into boiling vessel and soaks, boils
Boiling, filtering afterwards, sterilization treatment obtain four kinds of auxiliary material extract solutions.
Step 3, biofermentation antibiosis liquid that step 1 and step 2 are obtained, kuh-seng, rheum officinale, rattletop, dandelion are carried
Liquid is taken to be well mixed by 3: 2: 2: 1: 1 weight proportion.
Made of above-mentioned preparation method " novel plant disease biocide ", it is using Chinese medicine as raw material, is sent out using biology
A kind of more effect plant disease biocides prepared by ferment engineering technology and Biochemical Engineering technology, can effectively anti-kill causes plant simultaneously
The pathogenic microorganisms such as fungi, bacterium and the virus of disease, and to the explant in plant cell engineering culture and cell and greatly
The equal non-hazardous effect of growth of crop seedling and explant in canopy, securely and reliably.And no matter during production and use,
Equal contamination-free produces.It is that the preferable of widely used similar drugs preparation substitutes production in tissue cultures and cell culture at present
Product.
The present invention has effect good, the characteristics of having no toxic side effect.Energy is safe efficient, quick, it is micro- by causing a disease widely to kill
Plant, Chinese medicine, the plant disease of anti-season fruits and vegetables caused by biology, it is that the novel plant disease that national society's planting industry is badly in need of resists
Raw agent product, market prospects are boundless.
Brief description of the drawings
Fig. 1 is the process chart of the embodiment of the present invention
Embodiment
Embodiments of the invention are illustrated below in conjunction with the accompanying drawings：
Refer to the attached drawing 1, in embodiments of the present invention, main material pseudo-ginseng, felwort, radix stellariae dichotomae are washed respectively first, soaked
After 24 hours, the pure water for the raw material addition 90% being put into boiling vessel by 10% boils bubble, afterwards by three kinds of gained decoctions
Nutrient solution is made by 1: 4: 2 part by weight is well mixed after filtered, sterilizing, is then added in every 1000ml nutrient solutions
(NH4)2HPO40.8g, KH2PO40.5g, MgSO40.05g, NaCl 1g, FeSO40.03g, sucrose 3g and glucose 1g.It is divided in
In 5 bottles of 500ml triangular flasks, every bottle of 200ml.Autoclaving, after cooling, inoculates that " aspergillus oryzae Aspergillus oryzae " are simultaneously
Cultivation and fermentation, 30 ± 1 DEG C of cultivation temperature, incubation time 12 days.So as to produce the core antibiosis liquid that can kill pathogenic microorganisms, then
Sterilization treatment is carried out to the antibiosis liquid and obtains main material extract solution --- " biofermentation antibiosis liquid "；
Then, auxiliary material kuh-seng, rheum officinale, rattletop, dandelion are washed respectively, soaked 24 hours, be put into boiling vessel and soak
Steep, boil, filtering afterwards, sterilization treatment obtain four kinds of auxiliary material extract solutions.Finally, then by the biofermentation antibiosis liquid obtained,
Kuh-seng, rheum officinale, rattletop, dandelion extract solution are well mixed by 3: 2: 2: 1: 1 weight proportion, and novel plant disease is obtained after sterilizing
Evil biocide.
Embodiments of the invention are to draw pseudo-ginseng, felwort, silver using bio-fermentation engineering and Biochemical Engineering technology
Active ingredient in the Chinese medicine materials such as radix bupleuri, kuh-seng, rheum officinale, rattletop, dandelion, this more effect novel plants being configured to
Disease biocide, simultaneously effectively anti-the pathogenic microorganisms such as the fungi for causing plant disease, bacterium and virus can be killed, but it is thin to plant
Explant in born of the same parents' engineering culture and the equal non-hazardous effect of the crop seedling in cell and greenhouse and explant.To micro- by causing a disease
Plant, Chinese medicine, the plant disease of anti-season fruits and vegetables caused by biology, energy is safe efficient, quick, is widely killed, and is to work as
The new biocide product that preceding planting industry is badly in need of, and the widely used similar drugs system in tissue cultures and cell culture at present
The preferable substitute products of agent.
Embodiments of the invention have been used to plant tissue culture and cell culture, as legume, orchid, Magnoliaceae are planted
The plant disease processing of thing and birch class plant etc..For anti-seasons such as pseudo-ginseng, dendrobium candidum, anti-season cucumber, tomato, color green peppers
Fruits and vegetables and rare traditional Chinese medicine etc..
It is three concrete application cases below：
1. seed disinfection
Vegetable seeds sterilization is handled using dual factors, and concentration gradient 1%, 2%, 3%, the time uses and sterilized respectively
3min, 5min, 8min, 12min, 20min find optimal disinfection concentration and time.The medicine is now with the current, need to be matched somebody with somebody with sterilized water, often
Sterilized water washing by soaking will be used after secondary seed disinfection three times, remain biocide.Seed disinfection standard should be thorough, again
Do not influence its germination percentage, only see seed sprout after whether microbiological contamination, could distinguish.
Specific design form is as follows：
| 1% | 2% | 3% | |
| 3min | 1 | 2 | 3 |
| 5min | 4 | 5 | 6 |
| 8min | 7 | 8 | 9 |
| 12min | 10 | 11 | 12 |
| 20min | 13 | 14 | 15 |
15 groups of experiments are designed altogether, and every group of experiment sterilizes 20 seeds, sow 5 bottles.
2. explant (stem section) sterilizes
Stem section sterilization uses dual factors test, concentration 1%, 2%, 3%, and disinfecting time is respectively 3mi n, 5min,
8min, 12min, 20min find optimal disinfection concentration and time.The medicine is now with the current, need to be matched somebody with somebody with sterilized water, each stem section sterilization
After will use sterilized water washing by soaking three times, remain biocide.The standard of stem section sterilization, is the stem section quilt that detects by an unaided eye
After biocide processing, stem section tender leaf and stem apex are not degree by browning, and the sterilization to stem section will also do repetition experiment twice, that is, treat
After the completion of stating method, above-mentioned processing is repeated once every three days and eight days.Main purpose is killed to more obstinate, not dead
The spore died, after it is sprouted into mycelium, then row is killed.
| 1% | 2% | 3% | |
| 3min | 1 | 2 | 3 |
| 5min | 4 | 5 | 6 |
| 8min | 7 | 8 | 9 |
| 12min | 10 | 11 | 12 |
| 20min | 13 | 14 | 15 |
15 groups of experiments are designed altogether, and every group connects 5 bottles, and every bottle connects 5 stem sections.
3. greenhouse cultivation
Greenhouse cultivation, 1: 800,1: 900,1: 1,000 three concentration of configuration amount, the seedbed of 5 square metres of sprinkling, add one piece
5m2Normal culture is CK.Totally four pieces of experimental plots.The seedling that object is firm bottle outlet is sprayed, culture period is 6 months, observes its seedling diseases
Evil situation.The standard of sterilization, it is to detect by an unaided eye after seedling handled by biocide, Miao Ye is not degree by browning.
| 1: 800,5m2 | 1: 900,5m2 | 1: 1000,5m2 | Ck, 5m2 |
Remarks：Once, the spray same day can not water for sprinkling in every 10 days.
Claims (1)
1. the preparation method of plant disease biocide, it is characterised in that this method comprises the following steps：
Step 1, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae are respectively put into boiling vessel and soaks, boil, afterwards
By three kinds of gained decoctions are filtered, nutrient solution is made by defined part by weight is well mixed after sterilizing, then in nutrient solution
Matched by specific " volume/weight " and add (NH4)2HPO4、KH2PO4、MgSO4、NaCl、FeSO4, sucrose and glucose；And will training
Nutrient solution carries out inoculated and cultured fermentation, so as to produce the core antimicrobial fluid that can kill pathogenic microorganisms, then the antimicrobial fluid is gone out
Bacterium processing obtains main material extract solution --- " biofermentation antibiosis liquid "；
Step 2, by cleaned auxiliary material kuh-seng, rheum officinale, rattletop, dandelion, it is respectively put into boiling vessel and soaks, boils, it
Filtering, sterilization treatment obtain four kinds of auxiliary material extract solutions afterwards；
Step 3, the main and auxiliary raw material extract solution that step 1 and step 2 are obtained are well mixed by weight ratio；The culture
The pseudo-ginseng of liquid, felwort, three kinds of extract solutions of radix stellariae dichotomae mixed proportion by weight are 1 part of pseudo-ginseng, 4 parts of felwort, 2 parts of radix stellariae dichotomae；
In the step 1 inoculated and cultured fermentation operation, (NH is added per 1000ml nutrient solutions4)2HPO40.5g~1g,
KH2PO40.3g~0.8g, MgSO40.03g~0.08g, NaCl 0.5g~1.5g, FeSO40.02g~0.05g, sucrose 2g~
6g and glucose 0.5g~1.5g；30 ± 1 DEG C of cultivation temperature, incubation time 7~14 days；
The biocide is mixed by weight ratio by following raw materials according：It is 3 parts of biofermentation antibiosis liquid, 2 parts of Radix Sophorae Flavescentis extractive liquid, big
Yellow 2 parts of extract solution, 1 part of rattletop extract solution, 1 part of dandelion extract solution；
The fermented bacterium of the biofermentation antibiosis liquid is：" aspergillus oryzae Aspergillus oryzae ".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510116696.9A CN104782675B (en) | 2015-03-18 | 2015-03-18 | Novel plant disease biocide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510116696.9A CN104782675B (en) | 2015-03-18 | 2015-03-18 | Novel plant disease biocide and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104782675A CN104782675A (en) | 2015-07-22 |
| CN104782675B true CN104782675B (en) | 2017-11-21 |
Family
ID=53548231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510116696.9A Expired - Fee Related CN104782675B (en) | 2015-03-18 | 2015-03-18 | Novel plant disease biocide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104782675B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105724449A (en) * | 2016-01-27 | 2016-07-06 | 王代春 | Traditional Chinese medicine capable of treating and preventing plant virus diseases and application method |
| CN105831166A (en) * | 2016-04-29 | 2016-08-10 | 山东胜伟园林科技有限公司 | Microbial pesticide special for preventing and controlling wheat diseases and pests and preparation method thereof |
| CN107258842A (en) * | 2017-07-20 | 2017-10-20 | 六盘水师范学院 | It is a kind of to prevent and treat Prospect on Kiwifruit Bacterial Canker and the medicament and preparation method of blossom rot |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
| CN101322746A (en) * | 2008-05-09 | 2008-12-17 | 周应揆 | Secondary fermentation method natural medicinal preparation, preparation and use |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
-
2015
- 2015-03-18 CN CN201510116696.9A patent/CN104782675B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
| CN101322746A (en) * | 2008-05-09 | 2008-12-17 | 周应揆 | Secondary fermentation method natural medicinal preparation, preparation and use |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
Non-Patent Citations (1)
| Title |
|---|
| 中国中草药源农药的应用开发进展;陈阳峰等;《农药》;20131010;第52卷(第10期);第717-720页；第719页左栏第4-6段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104782675A (en) | 2015-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105483053B (en) | A kind of microbial composite bacteria and its preparation method and application for preventing and treating Citrus Huanglongbing pathogen | |
| CN102964178A (en) | Composite microbial bactericide, and preparation method and application thereof | |
| CN101697740A (en) | Novel traditional Chinese medicine pesticide and production technology thereof | |
| CN107473881A (en) | A kind of preparation method for the bio-feritlizer for improveing strawberry continuous cropping soil | |
| CN103039491A (en) | Composition containing chitosan oligosaccharide and prochloraz | |
| CN106465734A (en) | A kind of preventing and treating tobacco bacterial wilt fermentation liquid and preparation method thereof | |
| CN104782675B (en) | Novel plant disease biocide and preparation method thereof | |
| CN107439180A (en) | A kind of implantation methods for suppressing radix polygonati officinalis continuous cropping obstacle | |
| CN110352989A (en) | The new application of purple perilla volatile oil | |
| CN101223879B (en) | Method of preparing microorganisms pesticide for preventing vegetable fungi disease | |
| CN105385623A (en) | Actinomycete strain JSX-1 for preventing and curing phytophthora sojae and fungicide thereof | |
| CN104962289B (en) | Soil conditioner and preparation method thereof and application method | |
| CN101502275A (en) | Plant source pesticide | |
| CN105831162A (en) | Sprouting vegetable seed soaking fungicide and application thereof | |
| CN103858917A (en) | Mildew-resistant liquid used for seeds | |
| CN110250218A (en) | The new application of Herba Menthae Haplocalycis volatile oil | |
| CN110432288A (en) | Application of the Metarhizium anisopliae CQMa421 in the medicament of preparation prevention and treatment bean sprout rot disease | |
| CN109287677A (en) | The purposes of flos caryophylli volatile oil | |
| CN102626091A (en) | Application of isothiazolinone compounds in control of gray mold and sclerotinia rot of cucumber | |
| CN108142450B (en) | Herba schizonepetae extract and application thereof as plant antibacterial | |
| CN110269069A (en) | The new application of Fructus Foeniculi volatile oil | |
| CN104542135A (en) | Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide | |
| CN113598201B (en) | Botanical biocontrol bacterium preparation for preventing and treating rubber anthrax | |
| CN103382451B (en) | Streptomyces griseochromogenes YN-6 and application thereof to club root control | |
| CN103238633B (en) | Prevention solution for preharvest disease of Huping jujube and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhou Yingkui Document name: Notification of Passing Examination on Formalities |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171121 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |