CN104782675A - Novel plant disease antibiotic agent and preparation method thereof - Google Patents
Novel plant disease antibiotic agent and preparation method thereof Download PDFInfo
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- CN104782675A CN104782675A CN201510116696.9A CN201510116696A CN104782675A CN 104782675 A CN104782675 A CN 104782675A CN 201510116696 A CN201510116696 A CN 201510116696A CN 104782675 A CN104782675 A CN 104782675A
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- plant disease
- biocide
- antibiotic agent
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Abstract
A novel plant disease antibiotic agent and a preparation method thereof are provided; effective components in panax notoginseng, gentiana scabra bge, radix stellariae, radix sophorae flavescentis, rheum officinale, rhizoma cimicifugae, dandelion and other traditional Chinese medicine raw materials are acquired by applying a bio-fermentation engineering technology and a biochemical engineering technology, and are made into the novel multifunctional plant disease antibiotic agent according to a specific process and formula. The antibiotic agent can effectively resist and kill fungi, bacteria, viruses and other pathogenic microorganisms causing plant diseases simultaneously, but has no harmful effects on growth of explants and cells in plant tissue culture and cell engineering culture and growth of greenhouse crop seedlings and explants. Moreover, no matter in the process of production or use, no pollutant is generated. The antibiotic agent is an ideal substitute product for same drug preparations widely used in tissue culture and cell culture. The antibiotic agent has the characteristics of good effects and no toxic or side effect, can safely, efficiently, fast and widely kill the plant diseases of plants, Chinese herbal medicines, and anti-season fruits and vegetables caused by the pathogenic microorganisms, is a novel plant disease antibiotic agent product badly in need of national social planting industry, and has quite broad market prospects.
Description
Technical field
The invention belongs to the modern Chinese herbal medicine manufacturing technology field of applying biological fermentation engineering and Biochemical Engineering technology, is particularly novel plant disease biocide of raw material and preparation method thereof with traditional Chinese medicine.
Background technology
Novel plant disease biocide has safe, efficient, fast feature, also has can resist to kill multiplely to cause the fungi of plant disease, the function of the pathogenic microorganisms such as bacterium and virus simultaneously.Can be widely used in and anti-kill Plant Tissue Breeding, cell chulture and the high traditional Chinese medicine of greenhouse cultivation economic worth and anti-season fruits and vegetables plant disease.It is better than at present at tissue cultures, the antibacterial agricultural chemicals used in cell chulture and booth and preparation.And biocidal efficacies is also better than now widely used for Plant Tissue Breeding, cell chulture and U.S. PPM are anti-kills agent product.
Novel plant disease biocide has following advantage: 1. consumption is little; 2. little to the damage of plant explants, callus and cell; 3. noresidue.
Summary of the invention
The object of this invention is to provide a kind of novel plant disease biocide and preparation method.Existing for plant antibiotic in the hope of substituting on market with this biocide, come anti-to kill the pathogenic microorganisms such as fungi, bacterium and virus causing plant disease, and crop seedling in explant in cultivating plant cell engineering and cell and booth and explant are all without damaging effect.
Concrete technology preparation process of the present invention is such:
Step one, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae are put into immersion in boiling vessel respectively, boiled, afterwards by three kinds of gained liquids after filtration, mix after sterilizing by 1: 4: 2 part by weight and make culture fluid, then in culture fluid, add (NH by specific " volume/weight " proportioning
4)
2hPO
4, KH
2pO
4, MgSO
47H
2o, NaC1, FeSO
47H
2o, sucrose and glucose; Again culture fluid is carried out inoculated and cultured fermentation, thus produces the core antibiosis liquid killing pathogenic microorganisms, then sterilization treatment acquisition main material extract is carried out to this antibiosis liquid---" biofermentation antibiosis liquid ";
The fermented bacterium of biofermentation antibiosis liquid is: " aspergillus oryzae Aspergillus oryzae ".
In the inoculated and cultured fermentation operation described in this step, every 1000ml culture fluid adds (NH
4)
2hPO
40.5g ~ 1g, KH
2pO
40.3g ~ 0.8g, MgSO
40.03g ~ 0.08g, NaC10.5g ~ 1.5g, FeSO
40.02g ~ 0.05g, sucrose 2g ~ 6g and glucose 0.5g ~ 1.5g; Cultivation temperature 30 ± 1 DEG C, incubation time 7 ~ 14 days.
Step 2, by cleaned auxiliary material kuh-seng, rheum officinale, rattletop, dandelion, put into immersion in boiling vessel respectively, boil, filtration, sterilization treatment obtain four kinds of auxiliary material extracts afterwards.
Step 3, biofermentation antibiosis liquid step one and step 2 obtained, kuh-seng, rheum officinale, rattletop, dandelion extract mix by the weight proportion of 3: 2: 2: 1: 1.
" the novel plant disease biocide " be made up of above-mentioned preparation method, take traditional Chinese medicine as raw material, one many effects plant disease biocide prepared by applying biological fermentation engineering and Biochemical Engineering technology, simultaneously effectively anti-ly can kill the pathogenic microorganisms such as fungi, bacterium and virus causing plant disease, and the growth of the explant in cultivating plant cell engineering and the crop seedling in cell and booth and explant is all without damaging effect, safe and reliable.And no matter in production and use procedure, equal contamination-free produces.The desirable substitute products of widely used similar drugs preparation in tissue cultures and cell chulture at present.
The present invention has effective, the feature had no side effect.Can safety, efficiently, fast, widely kill caused by pathogenic microorganisms plant, traditional Chinese medicine, anti-season fruits and vegetables plant disease, be the novel plant disease biocide product that national society's plant husbandry is badly in need of, market prospects are boundless.
Accompanying drawing explanation
Fig. 1 is the process chart of the embodiment of the present invention
Embodiment
Below in conjunction with accompanying drawing, embodiments of the invention are described:
With reference to accompanying drawing 1, in embodiments of the present invention, first after main material pseudo-ginseng, felwort, radix stellariae dichotomae being washed respectively, soaking 24 hours, put into the pure water adding 90% by the raw material of 10% in boiling vessel to boil, afterwards by three kinds of gained liquids after filtration, mix after sterilizing by 1: 4: 2 part by weight and make culture fluid, then in every 1000ml culture fluid, add (NH
4)
2hPO
40.8g, KH
2pO
40.5g, MgSO
40.05g, NaCl 1g, FeSO
40.03g, sucrose 3g and glucose 1g.Be divided in 5 bottles of 500ml triangular flasks, every bottle of 200ml.Autoclaving, after cooling, inoculates " aspergillus oryzae Aspergillus oryzae " and cultivation and fermentation, cultivation temperature 30 ± 1 DEG C, incubation time 12 days.Thus produce the core antibiosis liquid killing pathogenic microorganisms, then sterilization treatment acquisition main material extract is carried out to this antibiosis liquid---" biofermentation antibiosis liquid ";
Then, auxiliary material kuh-seng, rheum officinale, rattletop, dandelion are washed respectively, soak 24 hours, put into immersion in boiling vessel, boil, filtration, sterilization treatment obtain four kinds of auxiliary material extracts afterwards.Finally, then obtained biofermentation antibiosis liquid, kuh-seng, rheum officinale, rattletop, dandelion extract are mixed by the weight proportion of 3: 2: 2: 1: 1, after sterilizing, obtain novel plant disease biocide.
Embodiments of the invention are the active ingredient that applying biological fermentation engineering and Biochemical Engineering technology draw in the Chinese medicine materials such as pseudo-ginseng, felwort, radix stellariae dichotomae, kuh-seng, rheum officinale, rattletop, dandelion, this many effects novel plant disease biocide be mixed with, simultaneously effectively anti-ly can kill the pathogenic microorganisms such as fungi, bacterium and virus causing plant disease, but crop seedling in explant in cultivating plant cell engineering and cell and booth and explant are all without damaging effect.To the plant caused by pathogenic microorganisms, traditional Chinese medicine, anti-season fruits and vegetables plant disease, can safety, efficiently, fast, widely killed, being the new biocide product that current plant husbandry is badly in need of, is also the desirable substitute products of widely used similar drugs preparation in tissue cultures and cell chulture at present.
Embodiments of the invention for plant tissue culture and cell chulture, as the plant disease process of leguminous plant, orchid, Magnoliacea plant and Betulaceae class plant etc.For pseudo-ginseng, dendrobium candidum, anti-season cucumber, tomato, the anti-season such as color green pepper fruits and vegetables and rare traditional Chinese medicine etc.
Below three embody rule cases:
1. seed disinfection
Plant seed sterilization adopts dual factors process, and concentration gradient is 1%, 2%, 3%, and the time adopts sterilize respectively 3min, 5min, 8min, 12min, 20min to find optimum disinfection concentration and time.This medicine is now with the current, need join with sterile water, all uses sterile water washing by soaking three times after each seed disinfection, does not make biocide remain.Seed disinfection standard should be thorough, do not affect its germination rate again, only to see after seed germination whether microbiological contamination, just can distinguish.
Specific design form is as follows:
| 1％ | 2％ | 3％ | |
| 3min | 1 | 2 | 3 |
| 5min | 4 | 5 | 6 |
| 8min | 7 | 8 | 9 |
| 12min | 10 | 11 | 12 |
| 20min | 13 | 14 | 15 |
Design 15 groups of experiments altogether, often group experiment sterilization 20 seeds, sow 5 bottles.
2. explant (stem section) sterilization
The sterilization of stem section adopts dual factors test, and concentration is 1%, 2%, 3%, and disinfecting time is respectively 3mi n, and 5min, 8min, 12min, 20min find optimum disinfection concentration and time.This medicine is now with the current, need join with sterile water, all uses sterile water washing by soaking three times, do not make biocide remain after each stem section sterilization.The standard of stem section sterilization, be detect by an unaided eye stem section by after biocide process, stem section tender leaf and stem apex, not by brown degree of turning to, also will do repeated test twice to the sterilization of stem section, namely after said method completes, repeat once above-mentioned process again every three days and eight days.Main purpose to kill the spore to more obstinate, not dead, sprout into after mycelium, then row killed until it.
| 1％ | 2％ | 3％ | |
| 3min | 1 | 2 | 3 |
| 5min | 4 | 5 | 6 |
| 8min | 7 | 8 | 9 |
| 12min | 10 | 11 | 12 |
| 20min | 13 | 14 | 15 |
Design 15 groups of experiments altogether, often group connects 5 bottles, every bottle graft 5 stem sections.
3. greenhouse cultivation
Greenhouse cultivation, configuration amount is 1: 800,1: 900,1: 1,000 three concentration, sprays the seedbed of 5 square metres, adds one piece of 5m
2normal cultivation is CK.Totally four pieces of experimental field.Spray the seedling that object is firm bottle outlet, culture period is 6 months, observes its Seedling Diseases situation.The standard of sterilization, be detect by an unaided eye seedling by after biocide process, Miao Ye is not by brown degree of turning to.
| 1∶800，5m 2 | 1∶900，5m 2 | 1∶1000，5m 2 | Ck，5m 2 |
Remarks: spray once for every 10 days, spray medicine can not water the same day.
Claims (5)
1. the preparation method of novel plant disease biocide, is characterized in that the method comprises the following steps:
Step one, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae are put into immersion in boiling vessel respectively, boiled, afterwards by three kinds of gained liquids after filtration, mix after sterilizing by the part by weight of regulation and make culture fluid, then in culture fluid, add (NH by specific " volume/weight " proportioning
4)
2hPO
4, KH
2pO
4, MgSO
4, NaCl, FeSO
4, sucrose and glucose; And culture fluid is carried out inoculated and cultured fermentation, thus produce the core antimicrobial fluid killing pathogenic microorganisms, then sterilization treatment acquisition main material extract is carried out to this antimicrobial fluid---" biofermentation antibiosis liquid ";
Step 2, by cleaned auxiliary material kuh-seng, rheum officinale, rattletop, dandelion, put into immersion in boiling vessel respectively, boil, filtration, sterilization treatment obtain four kinds of auxiliary material extracts afterwards;
Step 3, main and auxiliary raw material extract step one and step 2 obtained mixes by weight ratio.
2. novel plant disease biocide preparation method according to claim 1, is characterized in that: make described in step one pseudo-ginseng of culture fluid, felwort, radix stellariae dichotomae three kinds of extracts by weight mixed proportion be pseudo-ginseng 1 part, felwort 4 parts, radix stellariae dichotomae 2 parts.
3. novel plant disease biocide preparation method according to claim 1, it is characterized in that: in step one inoculated and cultured fermentation operation, every 1000ml culture fluid adds (NH
4)
2hPO
40.5g ~ 1g, KH
2pO
40.3g ~ 0.8g, MgSO
40.03g ~ 0.08g, NaCl 0.5g ~ 1.5g, FeSO
40.02g ~ 0.05g, sucrose 2g ~ 6g and glucose 0.5g ~ 1.5g; Cultivation temperature 30 ± 1 DEG C, incubation time 7 ~ 14 days.
4. the novel plant disease biocide made of preparation method according to claim 1, it is characterized in that, this biocide is mixed by weight ratio by following raw materials according: biofermentation antibiosis liquid 3 parts, Radix Sophorae Flavescentis extractive liquid 2 parts, Radix Et Rhizoma Rhei extract 2 parts, rattletop extract 1 part, dandelion extract 1 part.
5. novel plant disease biocide according to claim 4, is characterized in that: the fermented bacterium of biofermentation antibiosis liquid is: " aspergillus oryzae Aspergillus oryzae ".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510116696.9A CN104782675B (en) | 2015-03-18 | 2015-03-18 | Novel plant disease biocide and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510116696.9A CN104782675B (en) | 2015-03-18 | 2015-03-18 | Novel plant disease biocide and preparation method thereof |
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|---|---|
| CN104782675A true CN104782675A (en) | 2015-07-22 |
| CN104782675B CN104782675B (en) | 2017-11-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105724449A (en) * | 2016-01-27 | 2016-07-06 | 王代春 | Traditional Chinese medicine capable of treating and preventing plant virus diseases and application method |
| CN105831166A (en) * | 2016-04-29 | 2016-08-10 | 山东胜伟园林科技有限公司 | Microbial pesticide special for preventing and controlling wheat diseases and pests and preparation method thereof |
| CN107258842A (en) * | 2017-07-20 | 2017-10-20 | 六盘水师范学院 | It is a kind of to prevent and treat Prospect on Kiwifruit Bacterial Canker and the medicament and preparation method of blossom rot |
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| CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
| CN101322746A (en) * | 2008-05-09 | 2008-12-17 | 周应揆 | Secondary fermentation method natural medicinal preparation, preparation and use |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
-
2015
- 2015-03-18 CN CN201510116696.9A patent/CN104782675B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
| CN101322746A (en) * | 2008-05-09 | 2008-12-17 | 周应揆 | Secondary fermentation method natural medicinal preparation, preparation and use |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
Non-Patent Citations (2)
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| 薛广波: "《现代消毒学》", 31 July 2002, 人民军医出版社 * |
| 陈阳峰等: "中国中草药源农药的应用开发进展", 《农药》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105724449A (en) * | 2016-01-27 | 2016-07-06 | 王代春 | Traditional Chinese medicine capable of treating and preventing plant virus diseases and application method |
| CN105831166A (en) * | 2016-04-29 | 2016-08-10 | 山东胜伟园林科技有限公司 | Microbial pesticide special for preventing and controlling wheat diseases and pests and preparation method thereof |
| CN107258842A (en) * | 2017-07-20 | 2017-10-20 | 六盘水师范学院 | It is a kind of to prevent and treat Prospect on Kiwifruit Bacterial Canker and the medicament and preparation method of blossom rot |
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| CN104782675B (en) | 2017-11-21 |
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