CN104542135A - Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide - Google Patents
Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide Download PDFInfo
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Abstract
The invention discloses a method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide. The method includes: preparing the mixed Sr18 biological nematocide from 8-time concentrated aqueous solution of Sr18 and avermectin emulsion according to a weight part ratio of 7.5:2; subjecting soil to rotary tillage before use of the mixed Sr18 biological nematocide, dividing plots according to areas of test plots, ridging, taking corresponding Sr18 agent and avermectin emulsion according to the preparation ratio at the step (1), uniformly mixing, adding clean water for diluting by 10-20 times, adopting spraying equipment for soil spraying, transplanting in 3-5 days after application of the mixed Sr18 biological nematocide, and additionally applying the nematocide for 1-2 times to long-season vegetables in the middle period of planting by irrigating the nematocide along with water and keeping the dosage and proportion unchanged; detecting conditions of vegetable root-knot nematodiasis. Experiment results show that root-knot nematodiasis can be effectively controlled by the method, consumption and utilization frequency of avermectins are greatly reduced, the picking period of cucumbers is greatly prolonged by about 3 months, and fruit setting rate of tomatoes is increased by 30%.
Description
The present invention obtains project of national nature science fund project and subsidizes (bullets 31272019).
Technical field
The present invention relates to the compounding method of biopesticide, especially a kind of adopt in the method mixture of mixture Sr18 fungal bio-nematicide prevention and control root knot nematode disease evil make use of the short length of Sr18 preparation, speed is killed and the Avermectin lasting period is long advantage, by the synergistic effect between the two, compensate for unitary agent self not enough, overcome the pesticide resistance of nematode, improve the control effect of medicament, extend the crop picking period, increasing both production and income is obvious, expands the scope of application of medicament.
Background technology
Plant nematode is the universal important phytopathy original of a class, and its parasitic scope is wide, and environmental suitability is strong, and almost often kind of crop is all subject to the infringement of Plant nematode, wherein endangers root-knot nematode and cyst roundworm most serious of all to crop.Root-knot nematode can danger to vegetables, tobacco, peanut, fruit tree, grain, 2000 various plants such as ornamental plants and Chinese herbal medicine, be especially injured seriously with economic crops such as tomato, cucumber, watermelon, tobacco, peanuts.At present, the whole world has been found that the root-knot nematode of report has more than 90 to plant, China record generation root-knot nematode more than 50 plant, with Meloidogyne incognita (
meloidogyne incognita), peanut root-knot nematode (
m. arenaria), M hapla (
m. hapla), javanese root knot nematode (
m. javanica) etc. four kinds of common root-knot nematode occurrence injury the most general and serious, all there is generation in the vast warm area from China Hainan Island to Liaodong Peninsula, and harm is serious.The extent of injury of root-knot nematode is only second to fungi, has exceeded bacterium, virus becomes second largest plant disease, and the whole world reaches multi-million dollar because the harm of root-knot nematode is lost every year.The loss that root knot nematode disease evil causes in protecting field reaches 20-30%, and serious reaches 70%, even has no harvest.The regional protecting field such as North China, northwest, northeast and south China outdoor vegetable growing area root knot nematode disease have overflowed, and become the key constraints of protecting field development, prevention and control situation is very severe.
Vegetables are the first economic crops of China and maximum foreign exchange earning agricultural product.Development take facilities vegetable as the industrialized agriculture of representative is realize traditional farming to change to modern agricultural production mode, builds the important content of novel modern agriculture.The Eleventh Five-Year Plan period the Chinese government increases attention degree to industrialized agriculture and investment, and make China's facilities vegetable obtain swift and violent development, by the end of the year 2012, cultivated area will about reach 4,700,000 hm
2, account for 95% of China's facility cultivation and 80% area of world's facilities horticulture respectively, become the country that facility area is maximum in the world, and increasing with the speed of about 10% every year.China's facilities vegetable output value has reached 7,000 hundred million yuan, accounts for vegetables and the National Planting gross output value 65% and 20% respectively, has become the Major agro-industry in the many regions of China.Along with facilities vegetable is specialized and the development of scale; the Soil-sickness Problem caused primarily of reasons such as soil-borne disease insect pests also becomes increasingly conspicuous, and the phenomenon causing a large amount of underproduction of the crop such as cucumber, tomato even to ruin canopy because of root knot nematode disease and tomato yellow leaf curl virus constantly occurs in some regions.In addition, there is the problems such as kind is single, continuous cropping obstacle due to China's furnished equipments, cause root knot nematode disease and tomato yellow leaf curl virus to be caused harm day by day serious, become the bottleneck of the sustainable production of facilities vegetable, seriously constrained the development of China's facilities vegetable industry.It is reported; the generation of vegetables main producing region, Shandong Province vegetable at protected field root knot nematode disease is quite general, and the booth incidence of disease reaches 67. 6%, and the old canopy incidence of disease is close to 95%; serious regional production loss, more than 70%, has had a strong impact on the kind of local vegetables and output, has reduced the economic benefit of peasant household.Beijing has at least 23 small towns that nematodiasis occurs at present, and the protecting field area of being injured reaches 250,000 mu, particularly outstanding with the plantation garden of scale.Hebei province's protecting field gross area about 4,000,000 mu, wherein the melon-fruit-like vegetable area about 2,500,000 mu such as cucumber, tomato, eggplant, green pepper, by root-knot nematode harm about 1,500,000 mu, nearly 700,000 mu of serious harm area; According to another incomplete statistics, only in the In Guangdong Province based on sandy soil, the occurring area of root knot nematode disease is more than 15,000,000 mu, after disease occurs, general underproduction 10%-20%, seriously reaches more than 75% and even has no harvest, and some vegetable field is had to discarded or replanted other crop.According to estimates, China vegetables are every year because root-knot nematode endangers the super several hundred million unit of the loss caused.
Although China is thousands of family of pesticide producing enterprise at present, the existing kind more than 200 of pesticide species of production, can only lack nematocide.Therefore, exploitation nematocide has caused the great attention about agricultural chemicals scientific research department and manufacturing enterprise.Along with plant nematode disease is on the rise and the enhancing of people's environment and health perception, the domestic and international market demand to control plant nematode diseases biopesticide is very large at present, but lacks suitable biological prevention and control agent, there is very large blank in this respect.. the secondary metabolite of fungi is as second generation nematode bio-control factors, this kind of biopesticide plays a role in the mode directly acting on crops nematode, therefore has that effect is fast, drug effect is high, Environmental compatibility good, is not easy to cause the advantages such as secondary pollution and becomes the field that research is very popular at present both at home and abroad.Although the fungi reported kills line active metabolite a lot, this has wherein realized industrialization and has produced, as biopesticide for preventing and treating the then few of plant nematode diseases.In China, most nematode killing agent is high poison or poisoning chemical pesticide, and along with continuing to increase of Environmental protection, they are faced with the danger of forbidding; By contrast, biopesticide is then few, mainly live bacteria agent Paecilomyces lilacinus and metabolite Avermectin (not as nematocide registration) two kinds.As Long-Time Service same agricultural chemicals, inherently accelerate nematode to their resistance.Therefore, in the urgent need to developing novel, eco-friendly second generation nematode biological prevention and control agent agricultural chemicals, its potential market is huge.
The female article be published on plos one of the yellow literary composition of seminar in 2014: Wen-Kun Huang, Jian-Hua Sun, Jiang-Kuan Cui, Gang-Feng Wang, Ling-An Kong, Huan Peng, Shu-Long Chen, De-Liang Peng. Efficacy Evaluation of Fungus Syncephalastrum racemosum and ematicide Avermectin against the Root-Knot Nematode Meloidogyne incognita on Cucumber [J]. Plos One, 2014, 9 (2): 1-6.) article Chinese medicine fermentation-scale is little, formula is PS, do not add malt extract, what formed under PS culture medium condition mostly is acidic materials, be unfavorable for the generation of the insect killing substance worked in neutral conditions, and pH is transferred to 7.0 by ferment filtrate 1.0mol/LNaOH, drug effect can be reduced, increase soil salt to pollute.Do not fully demonstrate the control effects of Sr18 to nematode simultaneously, use proportioning improper relevant with medicament, due to experimental field for root-knot nematode falls ill serious booth, in soil, nematode density is large, as used Sr18 underdosage, can not nematode be controlled in effective range, nematode in soil can be caused to breed again and increase the weight of to infect harm to plant; Moreover spraying time aspect is very unreasonable yet, in transplanting dispenser in first 2 days, does not reach the Best Times of pharmacy effect, the effect of medicament therefore can not be made to be given full play to.
Summary of the invention
The present invention is directed to above-mentioned Problems existing to improve:
1, adopt 5t tank scale fermentation to produce, use PSM medium to the addition of domestic malt extract, and non-adjust pH before the use of concentrated medicament.Its advantage is: after (1) fermentation-scale amplifies, and output increases, and can expand application area; (2) Sr18 kills line activity is the coefficient result of multicomponent, add malt extract and be conducive to bacterial strain while accumulation acidic metabolite, produce the nematocidal active material worked in neutral conditions, thus improve its nematicide resultant effect, and use the cost of domestic malt extract will be significantly less than import malt extract; (3) pH is transferred to 7.0 by ferment filtrate 1.0mol/L NaOH, can reduce drug effect, increases soil salt and pollutes.
2, the proportioning of Sr18 and Avermectin is improved, increase the usage amount of Sr18, become 7.5:2 from original 5:2.Its advantage is: the insecticidal activity of (1) Sr18 nematode killing agent is high, and it is fast to play a role, plant strain growth can also be promoted, increase plant to the resistance of nematode infection, but its lasting period is short compared with Avermectin, and does not have systemic action, and the effect of Avermectin to nematode makes it benumb, can enter in plant and play a role, the two mixture uses can reach mutual supplement with each other's advantages; (2) before transplanting, suitably strengthen the dosage of Sr18, can be given full play to it and nematode speed to be killed and to plant growth promotion and the advantage that enhances disease resistance, thus the nematode population effectively reduced in soil and strengthen the ability that plant resists nematodiasis.
3, in application method, (1) improves spraying time, in advance 3-5 days process soil.Its advantage is: indoor insecticidal activity experiment confirms that the best use of time of Sr18 to pine wood nematode is 5 days, and root-knot nematode is different from pine wood nematode because body wall constructs, more responsive to medicament, so lethal time is shorter than pine wood nematode.Therefore dispenser in 3-5 days before transplanting, can give full play to its lethal effect to nematode in soil, effectively reduce insect density, reduces nematode infecting plant; (2) spraying times is improved according to nematode morbidity actual conditions, for long season vegetables, except carrying out except soil treatment before transplanting, planting mid-term, increase 1-2 medication, method is water with water, pharmaceutical quantities and constant rate, can effectively control root knot disease, greatly reduces dosage and the number of times of Avermectin, and substantially prolongs nearly 3 months of cucumber picking period, tomato fruit-setting rate increases by 30%.
The present invention is based on the needs of production practices, for domestic and international about nematode biological and ecological methods to prevent plant disease, pests, and erosion Problems existing at present, utilize the Syncephalastrum racemosum filamentous fungi Sr18 filtered out from soil, the technological condition for fermentation of its nematocidal active material and Sr18 fungal bio-nematicide and abamectin emulsifiable concentrate mixture are used for the biological Control Technology of plant root-knot nematodes evil, the biology that exploitation is used for the highly effective and safe of the nematodiasis be on the rise in treatment facility obstruction of vegetable continuous cropping kills nematode mixture, not only effectively can control insect density, simultaneously also can Promoting plant growth, significantly improve crop yield.
The present invention relates to the compounding method of biopesticide, especially mixture is carried out with Sr18 fungal bio-nematicide and abamectin emulsifiable concentrate, make use of the short length of Sr18 preparation in mixture, speed is killed and the Avermectin lasting period is long advantage, by the synergistic effect between the two, compensate for unitary agent self not enough, overcome the pesticide resistance of nematode, improve the control effect of medicament, extend the crop picking period, increasing both production and income is obvious, expands the scope of application of medicament.
Sr18 fungal bio-nematicide has heat-resisting, water-soluble, low toxicity, kill that nematode spectrum is wide, effect is fast, drug effect high, owing to being that various ingredients works, nematode not easily develops immunity to drugs, do the insect density that soil treatment effectively can reduce nematode, effect experiment confirms tomato and Cucumber root-knot nematode disease evil are had to good control efficiency and have to promote plant strain growth and obvious production-increasing function.But because said preparation belongs to non-internal-suction type, the lasting period is short compared with Avermectin, and the effect of Avermectin to nematode makes it benumb, and can enter in plant and play a role, the two is used with can reach mutual supplement with each other's advantages.
The present invention uses the PSM medium adding domestic malt extract, the Sr18 concentrate aqueous liquid through 5t tank scale fermentation manufacture: be used for prevention and control root knot nematode disease evil with Avermectin mixture.The proportioning of Sr18 fungal bio-nematicide and Avermectin is improved, increases the usage amount of Sr18 fungal bio-nematicide, become 7.5:2 from original 5:2.Improve spraying time, 3-5 days process soil in advance, spraying times is improved according to nematode morbidity actual conditions, for long period vegetables, planting mid-term, increase 1-2 medication, can effectively control root knot disease, greatly reduce dosage and the number of times of Avermectin, and substantially prolongs nearly 3 months of cucumber picking period, tomato fruit-setting rate increases by 30%.
For achieving the above object, the invention discloses following technical scheme:
1, adopt a method for mixture Sr18 fungal bio-nematicide prevention and control root knot nematode disease evil, it is characterized in that being undertaken by following step:
(1) being configured to of mixture Sr18 fungal bio-nematicide: 8 times of concentrate aqueous liquids of Sr18 and Avermectin emulsion are by ratio of weight and the number of copies for 7.5:2 prepares, and Sr18 fungal bio-nematicide usage amount is Sr18 7.5ml/m
2+ 1.8% abamectin emulsifiable concentrate 2ml/m
2, use after the consumption of 1.8% is converted to for the abamectin emulsifiable concentrate of other concentration.Described Sr18 fungal bio-nematicide refers to the Sr18 fungal bio-nematicide that 5t tank scale fermentation is produced, and its preparation method is shown in embodiment 1.
(2) application method: rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, corresponding Sr18 fungal bio-nematicide and abamectin emulsifiable concentrate is measured according to the configuration of step (1), after mixing, after adding clear water dilution 10 ~ 20 times, carry out soil sprinkling by the medical instruments such as sprayer, watering can, transplant after dispenser 3-5 days.For vegetables in short season, mainly within 3-5 days before transplanting, carry out soil treatment; Vegetables wherein refer to cucumber, tomato, celery, Kidney bean
.
, medicament fermentation standby:
Adopt 5t tank scale fermentation to produce, use PSM medium to the addition of domestic malt extract, and non-adjust pH before the use of concentrated medicament.Its advantage is: after (1) fermentation-scale amplifies, and output increases, and can expand application area; (2) Sr18 kills line activity is the coefficient result of multicomponent, add malt extract and be conducive to bacterial strain while accumulation acidic metabolite, produce the nematocidal active material worked in neutral conditions, thus improve its nematicide resultant effect, and use the cost of domestic malt extract will be significantly less than import malt extract; (3) pH is transferred to 7.0 by ferment filtrate 1.0mol/LNaOH, can reduce drug effect, increases soil salt and pollutes.
, Mixed Pharmacy proportioning:
The proportioning of Sr18 and Avermectin is improved, increases the usage amount of Sr18 fungal bio-nematicide, become 7.5:2 from original 5:2.Its advantage is: the insecticidal activity of (1) Sr18 nematode killing agent is high, and it is fast to play a role, plant strain growth can also be promoted, increase plant to the resistance of nematode infection, but its lasting period is short compared with Avermectin, and does not have systemic action, and the effect of Avermectin to nematode makes it benumb, can enter in plant and play a role, the two mixture uses can reach mutual supplement with each other's advantages; (2) before transplanting, suitably strengthen the dosage of Sr18, can be given full play to it and nematode speed to be killed and to plant growth promotion and the advantage that enhances disease resistance, thus the nematode population effectively reduced in soil and strengthen the ability that plant resists nematodiasis.
, application method:
(1) spraying time is improved, in advance 3-5 days process soil.Its advantage is: indoor insecticidal activity experiment confirms that the best use of time of Sr18 to pine wood nematode is 5 days, and root-knot nematode is different from pine wood nematode because body wall constructs, more responsive to medicament, so lethal time is shorter than pine wood nematode.Therefore dispenser in 3-5 days before transplanting, can give full play to its lethal effect to nematode in soil, effectively reduce insect density, reduces nematode infecting plant; (2) spraying times is improved according to nematode morbidity actual conditions, for long season vegetables, except carrying out except soil treatment before transplanting, planting mid-term, increase 1-2 medication, method is water with water, pharmaceutical quantities and constant rate, can effectively control root knot disease, greatly reduces dosage and the number of times of Avermectin, and substantially prolongs nearly 3 months of cucumber picking period, tomato fruit-setting rate increases by 30%.
The present invention is more detailed to be described below:
1 experimental technique:
1.1 root-knot nematode disease investigation
The mode such as on-site inspection, random sampling of employing, to the root-knot nematode of Beichen District, Tianjin, Xiqing District, Wuqing District, Baodi District, vegetables main producing region, Ji County, a situation arises investigates, and specify that the occurrence and distribution situation of the above-mentioned regional root-knot nematode in Tianjin.
1.2 Sr18 biological prevention and control agents and Avermectin mixing formula are on the impact of plant growth
Experiment one:
The sage village, Baodi District, Tianjin City
Experiment material: cucumber
Experimental period: 2012.10---2013.8
Rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, according to using dosage packing medicament, dilutes after adding proper amount of clear water, carries out dispenser by the medical instruments such as sprayer, watering can.Transplant after dispenser 3-5 days.
Have rated use totally 4 process at Baodi District, Tianjin City sage Zhuan Zhen:
No. 1 Sr18:10mL/m
2,
No. 2 5.0% Avermectin (Hebei agricultural chemicals Co., Ltd: solely high): 1.07mL/m
2,
No. 3 0.7mL/m
25.0% Avermectin (Hebei agricultural chemicals Co., Ltd: solely high)+7.5mL/m
2sr18,
No. 4 blanks
Experiment two:
The Hou Gan village, great Liang town, Wuqing District, Tianjin
Experimental period: 2012.12---2013.6
Experiment material: tomato
Rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, according to using dosage packing medicament, dilutes after adding proper amount of clear water, carries out dispenser by the medical instruments such as sprayer, watering can.Transplant after dispenser 3-5 days.
Have rated use totally 4 process in the Hou Gan village, great Liang town, Wuqing District, Tianjin:
No. 1 Sr18:10mL/m
2,
No. 2 1.8% Avermectin (Tianjin Chinese nation): 3 mL/m
2
No. 3 2mL/m
21.8% Avermectin (Tianjin Chinese nation)+7.5mL/m
2sr18,
No. 4 blanks
Experiment three:
Native place town, Ji County
Rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, according to using dosage packing medicament, dilutes after adding proper amount of clear water, carries out dispenser by the medical instruments such as sprayer, watering can.Transplant after dispenser 3-5 days.In the brooder test of pesticide effectiveness in native place town, Ji County, by Sr18 biological prevention and control agent and Avermectin conbined usage.
2 results
2.1 Vegetable producing region root-knot nematode Harm investigation
Investigate by a situation arises to the root-knot nematode of Beichen District, Tianjin, Xiqing District, Wuqing District, Baodi District, vegetables main producing region, Ji County, find Han Jiashu village, green light town, Beichen District, the blue or green Xi Zui village, town of Xiqing District willow, Hou Gan village, great Liang town, Wuqing District, regional root-knot nematode such as the sage village, area just outside a city gate town, Baodi District and native place village, native place town, Ji County, Jin Shuitun village, Xin Zhuan village etc. occur general, cause harm serious.Part plot root knot nematode disease strain rate reaches 100%, root knot index more than 60, average every hectogram soil nematodes quantity reaches 1386.3 (table 1, table 2, tables 3).
2.2 Sr18
biological prevention and control agent and Avermectin mixing formula are on the impact of plant growth
Experiment one:
The sage village, Baodi District, Tianjin City
Sr18 fungal bio-nematicide and Avermectin used in combination, suitably increase the amount of Sr18, effectively can control Cucumber root-knot nematode disease evil, control efficiency reaches more than 80%, and rate of growth reaches 52.4%.Sr18 and Avermectin process output are significantly higher than check plot output, can extend nearly 3 months of picking period.
Table 4 Sr18 fungal bio-nematicide is on the impact (Baodi) of the preventive effect of root-knot nematode disease and output
Experiment two:
The Hou Gan village, great Liang town, Wuqing District, Tianjin
In great Liang town, Wuqing District, result of the test shows (table 5), Sr18 fungal bio-nematicide and Avermectin used in combination, suitably add the amount of Sr18, can effectively control causing harm of tomato root-knot eelworm, control efficiency reaches 76.6%, and rate of growth reaches 57.7%.
Onsite acceptance Hui Xiaozeng state booth examination on May 28th, 2013 can receive more than 1000 jin of tomato the previous day, at least many receipts more than 10,000 yuan, and two original every canopies of canopy receive more than 20,000 yuan, amount to more than 40,000 yuan, take in and can reach more than 50,000 yuan after using Sr18+ Avermectin mixture.The morning of contrast seedling Huang, the receipts of administration eight rows, nine rows, have to 10 row fruits, to top, upper fruit is still very large.Examination group expert letter permanent teacher say happily: " project is well-done goes bad peasant has the final say, and peasant just proves ".
Table 5 Sr18 fungal bio-nematicide is on impact (the Hou Gan village, great Liang town, Wuqing of the preventive effect of root-knot nematode disease and output
)
Experiment three: native place town, Ji County
Show in the brooder test of pesticide effectiveness result in native place town, Ji County, by Sr18 biological prevention and control agent and Avermectin conbined usage, effectively can alleviate the harm of root-knot nematode, extend nearly 2 months of picking period, control efficiency can reach more than 85%, on average increases production 40%-50%.
May 23 in 2013 is investigated, and model household Wang Bao army reflects: nematode is maximum defect, can cause total crop failure time serious.Within 2012, (before not testing) root-knot nematode disease is serious, tomato has only received 4 fringes, 2013 except control group growing way obvious on the weak side except, administration group root does not find root-knot nematode, tomato growing way is fine, Miao Zhuan, fruit quality is excellent, sour and sweet palatability, sell very well, 6-8 fringe fruit can be received then, per mu yield 10,000 jin, volume increase 50%.Former years, root knot disease was very serious, had no root knot after using Mixed Pharmacy.
Accompanying drawing illustrates:
Fig. 1 is cucumber growing way figure after the mixture process of the sage village, Baodi District.In October, 2012 plants, and within 2013, booth administration on August 1 group cucumber still grows vigorous, and control root-knot nematode disease Be very effective, extends nearly 3 months of picking period.
Fig. 2 is tomato results figure after the mixture process of great Liang town, Wuqing District.In December, 2012 plants, and booth administration on May 23 group in 2013 tomato growth is vigorous, high yield, and can connect 9-10 row fruit, than former years, more receipts 3 arrange fruit, and peasant realizes increasing both production and income.
Fig. 3 is tomato growing way figure after the mixture process of native place town, Ji County.Within 2013, booth administration group root does not find root-knot nematode, and tomato grows fine, Miao Zhuan, extends nearly 2 months of picking period.
Embodiment
For ensureing that above and other objects of the present invention feature and advantage become apparent, below especially exemplified by preferred embodiment, and in conjunction with instantiation, the present invention is described in further detail.
Embodiment 1
The preparation of Sr18 fungal bio-nematicide:
(1) preparation of Sr18 bacterium activation culture and spore suspension: by the bacterial classification of preservation in ampoul tube access slant medium, 26 DEG C of quiescent culture 6d; Get the Sr18 bacterium inclined-plane after activation, add the spore on the aseptic washing lower inclined plane of 5ml, pour into and be added with in the triangular flask of bead, vibrate 1 minute, counting;
(2) 200L tank fermentation scale-up: adopt 50L fermentation tank to prepare first order seed as seeding tank: according to 2.75 × 10
6the spore suspension access that step (1) is prepared by/L spore concentration is equipped with in the seeding tank of 22L medium, cultivates 24h for 26 DEG C.Be equipped with in the 200L fermentation tank of 150L fermentation medium with 4% inoculum concentration by first order seed access, speed of agitator 198r/min, tank pressure 0.05MPa, throughput is 20% for principle to maintain dissolved oxygen level, fermentation time 40h; Described fermentation medium (w/w) is: 30% potato leaching juice+2% sucrose+0.2% import malt extract;
(3) 5t tank fermentation scale-up: utilize potato peeling machine, potato is peeled, microtome, leaching juice; 500L fermentation tank is adopted to prepare first order seed: with 2.794 × 10
6the spore suspension access of/L spore concentration is equipped with in the seeding tank of 250L medium, cultivate 24h for 26 DEG C, with 4% (w/w) inoculum concentration, first order seed access is equipped with in the 5t fermentation tank of 3.29t fermentation medium, speed of agitator 120r/min, tank pressure 0.03MPa, throughput is to maintain dissolved oxygen level 20% for principle, fermentation time 40h; Said fermentation medium refers to PS or PSM medium: peeled potatoes 300g liquor, sucrose 20g, running water 1000mL (wherein PSM adds domestic malt extract 2g), pH nature;
(4) preparation of concentrate aqueous liquid: after fermentation ends, is heated to 70 DEG C by bacterium liquid, and adopt plate-frame filtering to remove thalline, gained filtrate concentrates to obtain 8-10 times of weight concentrate aqueous liquid through vacuum concentration pan.
embodiment
2
:
Adopt the method for mixture Sr18 fungal bio-nematicide prevention and control Cucumber root-knot nematode disease evil, it is characterized in that being undertaken by following step:
(1) 8 times of concentrate aqueous liquids of the configuration of mixture Sr18 fungal bio-nematicide: Sr18 and Avermectin emulsion are by ratio of weight and the number of copies for 7.5:2 prepares;
(2) application method: rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, corresponding Sr18 fungal bio-nematicide and abamectin emulsifiable concentrate is measured according to the configuration of step (1), after mixing, add after clear water dilutes 10 times, carry out soil sprinkling by sprayer medical instruments, dispenser was transplanted after 3 days, for vegetables in short season, mainly within first 3 days, carry out soil treatment in transplanting;
(3) planting mid-term, increase by 2 medications, method is with water pouring, pharmaceutical quantities and constant rate;
(4) detect the situation of Cucumber root-knot nematode disease evil, result root knot index is 12.8, and control efficiency reaches about 82.4%, and cucumber production promoting rate reaches 52.4%.
Embodiment 3
Adopt the method for mixture Sr18 fungal bio-nematicide prevention and control tomato root-knot eelworm disease, it is characterized in that being undertaken by following step:
(1) 8 times of concentrate aqueous liquids of the configuration of mixture Sr18 fungal bio-nematicide: Sr18 and Avermectin emulsion are by ratio of weight and the number of copies for 7.5:2 prepares; Namely Sr18 and Avermectin emulsion medicament usage amount are Sr18 7.5ml/m
2+ 1.8%(w/w) abamectin emulsifiable concentrate 2ml/m
2
(2) application method: rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, corresponding Sr18 medicament and abamectin emulsifiable concentrate is measured according to the configuration of step (1), after mixing, add after clear water dilutes 20 times, carry out soil sprinkling by medical instruments, dispenser was transplanted after 5 days, for vegetables in short season, mainly within first 5 days, carry out soil treatment in transplanting;
(3) planting mid-term, increase by 1 medication, method is with water pouring, pharmaceutical quantities and constant rate;
(4) detect the situation of tomato root-knot eelworm disease, result control efficiency reaches 76.6%, and significantly improve tomato fruit-setting rate, output increase reaches 57.6%.
Claims (5)
1. adopt a method for mixture Sr18 fungal bio-nematicide prevention and control root knot nematode disease evil, it is characterized in that being undertaken by following step:
(1) 8 times of concentrate aqueous liquids of the configuration of mixture Sr18 fungal bio-nematicide: Sr18 and Avermectin emulsion are by ratio of weight and the number of copies for 7.5:2 prepares;
(2) application method: rotary tillage soil before use, according to ridging behind area division of cells, experimental plot, corresponding Sr18 fungal bio-nematicide and abamectin emulsifiable concentrate is measured according to the configuration of step (1), after mixing, after adding clear water dilution 10 ~ 20 times, carry out soil sprinkling by medical instruments, transplant after dispenser 3-5 days, for vegetables in short season, mainly within 3-5 days before transplanting, carry out soil treatment;
(3) for long season vegetables, except carrying out except soil treatment before transplanting, planting mid-term, increase 1-2 medication, method is with water pouring, pharmaceutical quantities and constant rate;
(4) situation of root knot nematode is detected; Described Sr18 fungal bio-nematicide refers to the Sr18 fungal bio-nematicide of 5t tank fermenting and producing.
2. the method for prevention and control root knot nematode disease evil described in claim 1, vegetables wherein refer to cucumber, tomato, celery, Kidney bean.
3. the method for prevention and control root knot nematode disease evil described in claim 1, wherein Sr18 and Avermectin emulsion medicament usage amount are Sr18 fungal bio-nematicide 7.5ml/m
2+ 1.8%(w/w) abamectin emulsifiable concentrate 2ml/m
2.
4. the method for prevention and control root knot nematode disease evil according to claim 1, wherein said medical instruments refer to and carry out soil sprinkling by sprayer, watering can.
5. adopt the method for mixture Sr18 fungal bio-nematicide prevention and control root knot nematode disease evil in the prolongation cucumber picking period described in claim 1, increase the application of tomato fruit-setting rate aspect.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234416A (en) * | 2016-07-11 | 2016-12-21 | 天津师范大学 | A kind of fungal bio-nematicide compositions and application thereof |
| CN109089672A (en) * | 2018-09-19 | 2018-12-28 | 中国农业科学院蔬菜花卉研究所 | A method of bactericidal agent for preventing and treating vegetables leaf diseases are sprayed to soil |
-
2015
- 2015-01-27 CN CN201510038753.6A patent/CN104542135A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234416A (en) * | 2016-07-11 | 2016-12-21 | 天津师范大学 | A kind of fungal bio-nematicide compositions and application thereof |
| CN109089672A (en) * | 2018-09-19 | 2018-12-28 | 中国农业科学院蔬菜花卉研究所 | A method of bactericidal agent for preventing and treating vegetables leaf diseases are sprayed to soil |
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