CN105284867A - Polypeptin copper complexing agent for preventing and treating bacterial angular leaf spot - Google Patents

Abstract

The invention discloses a polypeptin copper complexing agent for preventing and treating bacterial angular leaf spot and belongs to the technical field of biological prevention and control of plant diseases. The polypeptin copper complexing agent for preventing and treating the bacterial angular leaf spot is prepared by culturing bacillus megatherium and bacillus polymyxa through a photocatalysis fermentation method to prepare polypeptin and polymyxin, and complexing the polypeptin and the polymyxin with nano monovalent copper. The acting mechanism of the polypeptin copper complexing agent for preventing and treating the bacterial angular leaf spot is that after cell walls of pathogens are crushed by strong oxidization, copper ions are combined with proteins of cell membranes so that the proteins of the cell membranes of the pathogens are condensed and the breathing of the pathogens is stopped, and furthermore, the activity is inactivated; furthermore, microelements needed by the growth of crops are provided and the disease resistance of the crops is improved; the material is formed by fermenting natural bacillus and is matched with the microelements, including copper, zinc, manganese, magnesium and iron, needed by the growth of the plants, so that the polypeptin copper complexing agent is easily absorbed by plants after being used and is not remained, and is a biological toxin-free and residue-free prevention and treatment scheme of the plant bacterial angular leaf spot.

Description

A kind of circulin copper complexing agent preventing and treating bacillary angle spot

Technical field

The present invention relates to a kind of circulin copper complexing agent preventing and treating bacillary angle spot, can be used on pseudomonas, Xanthomonas are caused cucumber, watermelon, muskmelon, fresh kidney beans, cotton bacterial angular leaf spot prevent and treat, after the antibacterial protein of microbial metabolism and copper complexing, reach the effect of control, avoid excessively using chemical pesticide to cause soil, the pollution problem of water body, reach controlling disease to occur, strengthen crop antibody, maintain the ecological balance and not residual function, the function that the present invention relates to belongs to the technical field of agricultural disease control.

Background technology

The pathogene of bacterial leaf spot is caused to be pseudomonas, Xanthomonas, the crops such as normal harm cucumber, muskmelon, watermelon, fresh kidney beans, generally in management is ploughed preferably, disease incidence is at 20%-30%, when mistake is fertile or climatic factor can cause full garden disease to occur, plant cell death is caused time serious, the withered even whole strain of blade is dead, setting ratio is caused to decline when disease occurs, cause production declining between 30-40%, cause the situation of total crop failure time serious, morbidity preference temperature is 18 ~ 25 DEG C, and relative moisture is more than 75%.Many in rainfall, high humidity, hypsography low-lying, fertilizer management is improper, continuous cropping, during improper ventilation, morbidity is serious, belong to low temperature, high wet type disease, the survival period of pathogene soil reaches 1 ~ 2 year, the routes of infection, by rainwater, irrigation water, multiple infecting is carried out in insect or farming operation, when facility plastic greenhouse is cultivated, the high humidity of air, the blade face of crop usually condenses, the water that disease portion bacterial ooze can fall in the globule or air with leaf margin guttation and ceiling is carrier, infecting repeatedly, therefore the guttation amount when crop is many, the condensation duration is long, be conducive to the intrusion of this disease and popular, in order to prevent and treat this disease, general plantation family can use phonetic bacterium tpn, can kill, Jia Ruinong, first frost copper, the agricultural chemicals such as DT bacteriocidin are prevented and treated, the use of these agricultural chemicals, when concentration is little to the effect of pathogene not, easily cause the pesticide resistance of pathogene, and poisoning is easily produced when concentration is too high, the quality of output product is made after using, mouthfeel declines, cause outside the problem of residue of pesticide, long-term spraying pesticide in addition, also can cause the probiotics die in soil, lose the ecological balance of soil itself, soil after losing activity, the control that more agricultural chemicals carries out disease must be relied on to planting backward, systematic disease is finally caused to occur.

Present consumer is for nuisanceless, green food is paid attention to gradually, requirement for Detecting Pesticide standard is more and more higher, at home more because agricultural chemicals, the problem of antibiotic contaminated soil, the problem that food security causes is more and more serious, want these problems polluted of maintenance, need to spend more social resources and money, one how is provided to utilize microorganism, coordinate the function of the anti-bacteria of copper ion, produce nuisanceless, do not develop immunity to drugs, the pest control method more automatically can degraded by plant absorption or edaphon, the growth of pathogene not only can be suppressed to infect, trace element during can providing plant growth again supplements, it is a solution being badly in need of at present wanting on agricultural planting.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of circulin copper complexing agent preventing and treating bacillary angle spot, it is with bacillus polymyxa, bacillus megaterium, via photocatalysis fermentation method, produce out the circulin with antibiotic property, polymixin, circulin complexing nanometer monovalence copper, form circulin copper, the mechanism of action of the present invention is that the circulus of application polymixin can insert cell membrane, the monovalence copper of circulin complexing is brought in the cell of pathogene, copper ion can condense intracellular protein, destroy the function of some enzymes, cell respiration is obstructed, until death by suffocation, reach the function of inactivating pathogens, bacillus megaterium is phosphorus decomposing, potassium solubilizing bacteria, effectively untie the potassium in soil, phosphate fertilizer, improve soil environment to improve, improve crop to phosphorus, the absorption ratio of potash fertilizer, the resistance of enhancing itself, after bacillus megaterium enters soil, become the dominant microflora of soil fast, control nutrition and other resources of rhizosphere, pathogen is caused to lose space and the condition of existence, reduce the Population of pathogene, cut off and infect source, and then reach prevention and the double effects for the treatment of, advantage of the present invention not only can not produce environment to be polluted, the copper ion used is the nutrient of demand of plant growth, easily be absorbed and used by plants, it is the control agent of the harmless a kind of bacterial angular leaf spot viral disease of nature, except can inactivating pathogens with block its routes of infection function except, do not produce the problem of poisoning in use, more can increase the lignin of crop, cellulosic formation, strengthen the antibiotic property of crop itself, solve the problem of residue of pesticide, more soil condition can be improved, degrade through the problem of pesticide pollution, the present invention is the biocontrol agent that not only can become economic worth, the technical barrier of residue of pesticide and pathogene disease resistance can also be solved, reach green, the target of non-polluted planting.

The present invention is that to solve the problems of the technologies described above the technical scheme adopted be a kind of circulin copper complexing agent preventing and treating bacillary angle spot, its material mixture ratio and processing step as follows:

1, material:

(1), material I enriched medium: rape cake 25%, soybean cake 40%, bitter chrysanthemum 20%, brown sugar 7%, salt 2%, zinc sulphate 2%, manganese sulphate 1.7%, magnesium sulfate 2%, ferrous sulfate 0.3%;

(2), material II bacterial classification: bacillus megaterium 8000IU is a, and bacillus polymyxa 8000IU is a;

(3), material III complexing agent: complexing nano monovalence copper is a;

2, processing step:

(1), by material I enriched medium according to after ratio adjustment mixing, put into high-temperature sterilization pot, carry out sterilizing with 120 DEG C;

(2), further the red enriched medium of completing steps 1 is placed to be cooled to after 25 DEG C, be placed in the fermentation cauldron of glass, inoculation material II bacterial classification, carry out illumination fermentation 7d, irradiating optical wavelength between yeast phase is 450nm:660nm, and ratio is 2:8, the light of illumination 4500 lumen, fermentation temperature maintains 25 DEG C-28 DEG C, and fermented filtration residue, obtains suspension and is fermentation of bacillus liquid;

(3), by material III complexing agent, be slowly added dropwise in the fermentation of bacillus liquid of completing steps 2, until zymotic fluid becomes dark green, be a kind of circulin copper complexing agent preventing and treating bacillary angle spot;

Advantage of the present invention:

The present invention contrasts the advantage of prior art, the present invention be a kind of with the residue after rape seed, soybean extract oil for main material, the growth characteristics preparation of cooperation bacillus megaterium, bacillus polymyxa is deployed into enriched medium, application bacillus is to the susceptibility of light, use illumination catalytic way, produce a large amount of circulins, polymixin, antibacterial protein and other organized enzyme, again made by circulin and copper complexing, there is suppression Pseudomonas alba, the function of Xanthomonas campestris activity, its mechanism of action is by the cell wall of outside Strong oxdiative pathogene, cause cell wall injured after, monovalence copper is invaded pathogenic cell film, with the protein combination in cell membrane, form condensation and block the function of breathing, by the phosphorus decomposing enzyme of bacillus megaterium fermentation output object, potassium decomposing enzyme, decompose the effective element in soil, the nutritive element of plant growth is provided, strengthen resistance and the resistance against diseases of crop, and the function of organophosphorous pesticide can be decomposed, be formed as the dominant bacteria of soil, block the pathogene passed inside soil, form prevention and the double effects for the treatment of, the advantage that the present invention has, the raw material obtained and technological process simple, element used in addition is the trace element needed for plant, not only can increase outside the nutritional supplementation of plant, the pollution problem of soil pesticide can also be solved, problem that is residual and soil pollution is not produced after using, the governance role of the present invention to pathogene is by outside strong oxidation in addition, after causing pathogen cells wall injured, insert the cell membrane of pathogene again, cause the condensation of albumen, pathogene is allowed to lose the function of breathing, and then the activity of deactivation pathogeny body, solve the problem that pathogene develops immunity to drugs, it is a kind of product of worth large-scale promotion application, the problem that plantation at present relies on agricultural chemicals can be solved.

Accompanying drawing explanation

A kind of process flow diagram preventing and treating the circulin copper complexing agent of bacillary angle spot of Fig. 1

embodiment and case

1, case one: farm, Ruichang City, Jiangxi Province, simple plastic greenhouse is utilized to plant cucumber 15 mu of ground, when mid-April in this year, find that yellow water soaked spots appears in cucumber leaves, observe from blade back and have no the mould plaque-like of black, there is light transmission, finding this phenomenon Zhan Zheng garden 1/3rd, through being judged as cucumber bacterial angular leaf spot; Apply circulin copper complexing agent of the present invention 800 times of liquid to spray, sprayed again once after three days, after young leaves grows after 7 days, have no this illness to occur again, spray 1000 times of liquid every maintenance in 7 days, occur to having no angular leaf spot of cucumber when uprooting plants after their edible portions have been harvested, than former years, the experience of planting exceeds 15% to output backward;

2, case two: farm, Zhangjiagang, Suzhou, Jiangsu Province, application simple plastic greenhouse plantation Melon 10 booths, mid-June in this year, find that yellow spotting appears in muskmelon, vein is dark green, some blade is withered, the yellow of melon head does not tie melon, through being judged as the muskmelon angular leaf spot that pseudomonas syringae causes, applying circulin copper complexing agent of the present invention 1000 times of liquid sprays in blade face, after continued to spray once every 3 days, to emerge sprouting to the 10th day terminal bud, young leaves is normal, within 15th day, female flower starts to bloom, within 18th day, bear fruit successfully, change melon rate and be reduced to less than 10%, every 10 days backward, spray 2000 times of liquid to prevent and treat, occur to the phenomenon having no muskmelon angular leaf spot when uprooting plants after their edible portions have been harvested,

3, case three: organic farm, Dongshan Town, Suzhou City of Jiangsu Province, the outdoor plantation watermelon of application facility booth 50 mu, during April in this year, find irregular filemot scab appears in blade, adularescent bacterium bacterial ooze on blade back, this phenomenon accounts for 1/5th of overall cultivated area, through being judged as watermelon angular leaf spot, applying circulin copper complexing agent of the present invention 1000 times of liquid sprays once, sprayed again once after 3 days, young leaves is checked after 7 days, have no this illness to occur, sprayed 1500 times of liquid to prevent and treat as maintenance every 7 days backward, recurrence is had no when July gathers, the fruit tied has the mouthfeel rustled, sugariness is 14-17 degree, residue of pesticide are detected through local agriculture test department, do not detect residue of pesticide,

By above actual in the application in land for growing field crops, when the period that angular leaf spot is occurred frequently, apply a kind of circulin copper complexing agent foundation crop difference of preventing and treating bacillary angle spot of the present invention, different ratios is taked to spray, treatment infected plant serves the activity suppressing germ, and spray the protective liquid of low solubility at a fixed time, also can play outside preventive effect, separately can increase the local flavor of colourity with of fruit itself, increasing quality and the price of commodity fruit, is the solution of a kind of green non-poisonous angular leaf spot control be worthy to be popularized.

Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little amendment and perfect, therefore protection scope of the present invention is when being as the criterion of defining with claims.

Claims (2)

1. the circulin copper complexing agent of a control bacillary angle spot, it is characterized in that: by bacillus megaterium, bacillus polymyxa, be 450nm:660nm through optical wavelength, ratio is 2:8, the light-illuminating of illumination 4500 lumen, after producing out circulin, polymixin, then carry out complexing with nanometer monovalence copper and obtain a kind of circulin copper complexing agent preventing and treating bacillary angle spot.

2. a kind of circulin copper complexing agent preventing and treating bacillary angle spot as claimed in claim 1, is characterized in that adopting following materials and process step to make:

1), material: material I enriched medium, rape cake 25%, soybean cake 40%, bitter chrysanthemum 20%, brown sugar 7%, salt 2%, zinc sulphate 2%, manganese sulphate 1.7%, magnesium sulfate 2%, ferrous sulfate 0.3%; Material II bacterial classification, bacillus megaterium 8000IU is a, and bacillus polymyxa 8000IU is a; Material III complexing agent, complexing nano monovalence copper is a;

2), processing step:

(1), by the enriched medium of material I according to after ratio adjustment mixing, put into high-temperature sterilization pot, carry out sterilizing with 120 DEG C;

(2), further the red enriched medium of completing steps 1 is placed to be cooled to after 25 DEG C, be placed in the fermentation cauldron of glass, inoculation material II bacterial classification, carry out illumination fermentation 7d, irradiating optical wavelength between yeast phase is 450nm:660nm, and ratio is 2:8, the light of illumination 4500 lumen, fermentation temperature maintains 25 DEG C-28 DEG C, and fermented filtration residue, obtains suspension and is fermentation of bacillus liquid;

(3), by material III complexing agent, be slowly added dropwise in the fermentation of bacillus liquid of completing steps 2, until zymotic fluid becomes dark green, be a kind of circulin copper complexing agent preventing and treating bacillary angle spot.