CN103783092A - Biopesticide and preparation method thereof - Google Patents
Biopesticide and preparation method thereof Download PDFInfo
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- CN103783092A CN103783092A CN201410039325.0A CN201410039325A CN103783092A CN 103783092 A CN103783092 A CN 103783092A CN 201410039325 A CN201410039325 A CN 201410039325A CN 103783092 A CN103783092 A CN 103783092A
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- 230000000853 biopesticidal effect Effects 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 91
- 241000233866 Fungi Species 0.000 claims abstract description 45
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims description 80
- 240000005001 Paeonia suffruticosa Species 0.000 claims description 39
- 235000003889 Paeonia suffruticosa Nutrition 0.000 claims description 39
- 241000894006 Bacteria Species 0.000 claims description 36
- 241000222122 Candida albicans Species 0.000 claims description 21
- 229940095731 candida albicans Drugs 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 230000001546 nitrifying effect Effects 0.000 claims description 21
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 20
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 20
- 229910052700 potassium Inorganic materials 0.000 claims description 20
- 239000011591 potassium Substances 0.000 claims description 20
- 239000004570 mortar (masonry) Substances 0.000 claims description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 244000061456 Solanum tuberosum Species 0.000 claims description 10
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 5
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000000749 insecticidal effect Effects 0.000 abstract description 3
- 230000008635 plant growth Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 241001098109 Jacaranda mimosifolia Species 0.000 abstract 2
- 241000736199 Paeonia Species 0.000 abstract 2
- 235000006484 Paeonia officinalis Nutrition 0.000 abstract 2
- 241000751139 Beauveria bassiana Species 0.000 abstract 1
- 206010067482 No adverse event Diseases 0.000 abstract 1
- 230000002411 adverse Effects 0.000 abstract 1
- 230000000266 injurious effect Effects 0.000 abstract 1
- 244000144972 livestock Species 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 8
- 241000012249 Dendrolimus spectabilis Species 0.000 description 7
- 241000256602 Isoptera Species 0.000 description 7
- 239000003905 agrochemical Substances 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- -1 100 parts Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000321538 Candidia Species 0.000 description 1
- 206010008428 Chemical poisoning Diseases 0.000 description 1
- 241000248757 Cordyceps brongniartii Species 0.000 description 1
- 238000006697 Forster synthesis reaction Methods 0.000 description 1
- 241001539803 Magnusiomyces capitatus Species 0.000 description 1
- 241000223250 Metarhizium anisopliae Species 0.000 description 1
- 241000187562 Rhodococcus sp. Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 241000894007 species Species 0.000 description 1
Abstract
The invention belongs to the biotechnical field, and specifically relates to a biopesticide and a preparation method of the biopesticide. The insecticidal efficacy of the biopesticides is greatly improved by adding an extract of a Jacaranda mimosifolia branch and a peony root extract into a pesticide. The extract of the Jacaranda mimosifolia branch and the peony root extract have no toxic effect to human and livestock, so that when the biopesticide provided by the invention is used, people do not need to worry about adverse effect of the biopesticide on the human body. By further adding saccharomycetes after 6-7 days of microculture, not only are the microorganism species enriched, but also the organic components in the pesticide are increased to promote growth of plants. By using beauveria bassiana and green muscardine fungi in the biopesticide, injurious insects in forests, nursery gardens, lawns and fields and the like can be effectively prevented and treated. The biopesticide provided by the invention has the characteristics of efficiency, environment friendliness, no secondary toxicity, no residue and no pollution, maintained ecological balance and no public nuisance, is an important guarantee to realize green and ecological agriculture and green enterprises, and has a wide prospect.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of biopesticide and preparation method thereof.
Background technology
Along with the development of agricultural production, people urgently wish green consumption, and urgently wish the agricultural chemicals of a kind of nuisanceless, broad spectrum activity, wide region integrated control damage by disease and insect, to adapt to market and people's demand.At present, the agricultural chemicals that peasant uses, is that industrial chemicals is made mostly, although can kill the insect on crops, but its residue still has larger injury to human body, used the product of this chemical pesticide and often refused because of the domestic and international selling market of the too high quilt of remains of pesticide composition, cause many agricultural product unsalable.In addition, this chemical pesticide is toxic, also often causes people, animal pesticide poisoning accident.Vast farmers urgently wishes a kind of good disinsection effect, and people and animals are not damaged, and price cheap agricultural chemicals again to improve safety and the feature of environmental protection of agricultural product, reduces production costs simultaneously.
Summary of the invention
The shortcoming that the object of the invention is to overcome existing prior art, provides a kind of biopesticide and preparation method thereof, not only good disinsection effect, and to person poultry harmless, cheap.
The technical solution used in the present invention is: a kind of biopesticide, described biopesticide is by potassium fulvate, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder, China tree seed extracts, saccharomycete, blue flower principal columns of a hall branches and leaves extract and tree peony root extract are made, wherein the parts by weight of the required each raw material of biopesticide of preparation are respectively: potassium fulvate 3-4 part, Candida albicans powder 0.008-0.01 part, nitrifying bacteria powder 0.01-0.03 part, white muscardine fungi powder 0.1-0.2 part, green muscardine fungus powder 0.3-0.5 part, saccharomycete 0.01-0.03 part, China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, tree peony root extract 18-20 part.
A preparation method for biopesticide, comprises the following steps:
Step 1, choose disease-free tree peony root, after shredding, putting into mortar grinds, in mortar, add absolute ether afterwards, the volume of the absolute ether that every gram of tree peony root is corresponding is 2mL, the ultrasonoscope of afterwards extract being put into frequency and be 22kHz extracts, and has extracted rear filtration and with activated carbon decolorizing, has then put into Rotary Evaporators and heat, and be under the condition of 95-105 ℃, to collect evaporation phase in temperature, obtain tree peony root extract;
Step 2, China tree seed and blue flower principal columns of a hall branches and leaves are pulverized, and proceed to respectively and in mortar, grind to form homogenate, proceed to respectively afterwards in the centrifuge that rotating speed is 10000r/min centrifugal, obtain the extract of China tree seed extracts and blue flower principal columns of a hall branches and leaves, both extracts are mixed and stirred, wherein China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, obtain mixture I, for subsequent use;
Step 3, take Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder and saccharomycete according to the parts by weight of described each component, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder and green muscardine fungus powder are put into same medium to be cultivated, after 6-7 days, add saccharomycete, cultivate and obtain mix bacterium agent I after 15-20 days, for subsequent use;
Step 4, take potassium fulvate and tree peony root extract according to the parts by weight of described each component, afterwards will mixture I, mix bacterium agent I, potassium fulvate and tree peony root extract stir after mixing.
Described medium is made up of potato, sucrose and water, and wherein the parts by weight of each component are respectively: potato 19-21 part, sucrose 2-3 part, 100 parts, water.
Candida albicans in the present invention (
candidia albicans) being preserved in Chinese common micro-organisms culture presevation administrative center, preservation bacterium number is CGMCC 2.538.Nitrifying bacteria (
rhodococcus sp.) being preserved in Chinese Typical Representative culture collection center, preservation bacterium number is CCTCC AB 207886.White muscardine fungi (
beauveria brongniartii) being preserved in Chinese common micro-organisms culture presevation administrative center, preservation bacterium number is CGMCC 3.03574.Green muscardine fungus (
metarhizium anisopliae) being preserved in Chinese common micro-organisms culture presevation administrative center, preservation bacterium number is CGMCC 3.03676.Saccharomycete (
trichosporon capitatum) being preserved in Chinese Typical Representative culture collection center, preservation bacterium number is CCTCC AY 93140.Above five kinds of bacterial classifications are all bought in one bio tech ltd, source, Shaanxi.
beneficial effect
1, the present invention by adding blue flower principal columns of a hall branches and leaves extract and tree peony root extract in agricultural chemicals, greatly improve the insecticidal effect of biopesticide, and blue flower principal columns of a hall branches and leaves and tree peony root extract all do not have toxic action to people and animals, needn't worry human body to cause harmful effect when the present invention is used.
2, the present invention adds saccharomycete after cultivating 6-7 days in microorganism again, has not only enriched microbe species, and has increased the organic principle in agricultural chemicals, promotes the growth of plant.
3, the present invention in biopesticide, use white muscardine fungi and green muscardine fungus can effectively preventing forest, the insect such as the grub in nursery, lawn, farmland etc., corn borer, pine caterpillars, termite, locust.
4, biopesticide of the present invention have high efficiency, the feature of environmental protection, without secondary toxicity, noresidue, pollution-free, keep ecological balance, non-harmful feature, be to realize green ecological agricultural, the important guarantee of green enterprises, has bright prospects.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail, and following examples are to carry out under prerequisite in technical scheme of the present invention, and protection scope of the present invention is not limited to following embodiment.
A kind of biopesticide, described biopesticide is by potassium fulvate, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder, China tree seed extracts, saccharomycete, blue flower principal columns of a hall branches and leaves extract and tree peony root extract are made, the parts by weight of wherein preparing the required each raw material of biopesticide are respectively: potassium fulvate 3-4 part, Candida albicans powder 0.008-0.01 part, nitrifying bacteria powder 0.01-0.03 part, white muscardine fungi powder 0.1-0.2 part, green muscardine fungus powder 0.3-0.5 part, saccharomycete 0.01-0.03 part, China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, tree peony root extract 18-20 part.
A preparation method for biopesticide, comprises the following steps:
Step 1, choose disease-free tree peony root, after shredding, putting into mortar grinds, in mortar, add absolute ether afterwards, the volume of the absolute ether that every gram of tree peony root is corresponding is 2mL, the ultrasonoscope of afterwards extract being put into frequency and be 22kHz extracts, and has extracted rear filtration and with activated carbon decolorizing, has then put into Rotary Evaporators and heat, and be under the condition of 95-105 ℃, to collect evaporation phase in temperature, obtain tree peony root extract;
Step 2, China tree seed and blue flower principal columns of a hall branches and leaves are pulverized, and proceed to respectively and in mortar, grind to form homogenate, proceed to respectively afterwards in the centrifuge that rotating speed is 10000r/min centrifugal, obtain the extract of China tree seed extracts and blue flower principal columns of a hall branches and leaves, both extracts are mixed and stirred, wherein China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, obtain mixture I, for subsequent use;
Step 3, take Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder and saccharomycete according to the parts by weight of described each component, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder and green muscardine fungus powder are put into same medium to be cultivated, after 6-7 days, add saccharomycete, cultivate and obtain mix bacterium agent I after 15-20 days, for subsequent use;
Step 4, take potassium fulvate and tree peony root extract according to the parts by weight of described each component, afterwards will mixture I, mix bacterium agent I, potassium fulvate and tree peony root extract stir after mixing.
Described medium is made up of potato, sucrose and water, and wherein the parts by weight of each component are respectively: potato 19-21 part, sucrose 2-3 part, 100 parts, water.
Embodiment 1
A kind of biopesticide, described biopesticide is by potassium fulvate, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder, China tree seed extracts, saccharomycete, blue flower principal columns of a hall branches and leaves extract and tree peony root extract are made, the parts by weight of wherein preparing the required each raw material of biopesticide are respectively: 3 parts of potassium fulvates, 0.008 part, Candida albicans powder, 0.01 part, nitrifying bacteria powder, 0.1 part, white muscardine fungi powder, 0.3 part, green muscardine fungus powder, 0.01 part, saccharomycete, 9 parts of China tree seed extracts, 8 parts of blue flower principal columns of a hall branches and leaves extracts, 18 parts of tree peony root extracts.
A preparation method for biopesticide, comprises the following steps:
Step 1, choose disease-free tree peony root, after shredding, putting into mortar grinds, in mortar, add absolute ether afterwards, the volume of the absolute ether that every gram of tree peony root is corresponding is 2mL, the ultrasonoscope of afterwards extract being put into frequency and be 22kHz extracts, and has extracted rear filtration and with activated carbon decolorizing, has then put into Rotary Evaporators and heat, and be under the condition of 95 ℃, to collect evaporation phase in temperature, obtain tree peony root extract;
Step 2, China tree seed and blue flower principal columns of a hall branches and leaves are pulverized, and proceed to respectively and in mortar, grind to form homogenate, proceed to respectively afterwards in the centrifuge that rotating speed is 10000r/min centrifugal, obtain the extract of China tree seed extracts and blue flower principal columns of a hall branches and leaves, both extracts are mixed and stirred, wherein 9 parts of China tree seed extracts, 8 parts of blue flower principal columns of a hall branches and leaves extracts, obtain mixture I, for subsequent use;
Step 3, take Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder and saccharomycete according to the parts by weight of described each component, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder and green muscardine fungus powder are put into same medium to be cultivated, after 6 days, add saccharomycete, cultivate and obtain mix bacterium agent I after 15 days, for subsequent use;
Step 4, take potassium fulvate and tree peony root extract according to the parts by weight of described each component, afterwards will mixture I, mix bacterium agent I, potassium fulvate and tree peony root extract stir after mixing.
Described medium is made up of potato, sucrose and water, and wherein the parts by weight of each component are respectively: 19 parts of potatos, 2 parts of sucrose, 100 parts, water.
Embodiment 2
A kind of biopesticide, described biopesticide is by potassium fulvate, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder, China tree seed extracts, saccharomycete, blue flower principal columns of a hall branches and leaves extract and tree peony root extract are made, the parts by weight of wherein preparing the required each raw material of biopesticide are respectively: 4 parts of potassium fulvates, 0.01 part, Candida albicans powder, 0.03 part, nitrifying bacteria powder, 0.2 part, white muscardine fungi powder, 0.5 part, green muscardine fungus powder, 0.03 part, saccharomycete, 16 parts of China tree seed extracts, 13 parts of blue flower principal columns of a hall branches and leaves extracts, 20 parts of tree peony root extracts.
A preparation method for biopesticide, comprises the following steps:
Step 1, choose disease-free tree peony root, after shredding, putting into mortar grinds, in mortar, add absolute ether afterwards, the volume of the absolute ether that every gram of tree peony root is corresponding is 2mL, the ultrasonoscope of afterwards extract being put into frequency and be 22kHz extracts, and has extracted rear filtration and with activated carbon decolorizing, has then put into Rotary Evaporators and heat, and be under the condition of 105 ℃, to collect evaporation phase in temperature, obtain tree peony root extract;
Step 2, China tree seed and blue flower principal columns of a hall branches and leaves are pulverized, and proceed to respectively and in mortar, grind to form homogenate, proceed to respectively afterwards in the centrifuge that rotating speed is 10000r/min centrifugal, obtain the extract of China tree seed extracts and blue flower principal columns of a hall branches and leaves, both extracts are mixed and stirred, wherein 16 parts of China tree seed extracts, 13 parts of blue flower principal columns of a hall branches and leaves extracts, obtain mixture I, for subsequent use;
Step 3, take Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder and saccharomycete according to the parts by weight of described each component, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder and green muscardine fungus powder are put into same medium to be cultivated, after 7 days, add saccharomycete, cultivate and obtain mix bacterium agent I after 20 days, for subsequent use;
Step 4, take potassium fulvate and tree peony root extract according to the parts by weight of described each component, afterwards will mixture I, mix bacterium agent I, potassium fulvate and tree peony root extract stir after mixing.
Described medium is made up of potato, sucrose and water, and wherein the parts by weight of each component are respectively: 21 parts of potatos, 3 parts of sucrose, 100 parts, water.
Test 1
Choose each 200 of grub, corn borer, pine caterpillars, termite, locust on December 5th, 2013, be divided into A, B, C, tetra-groups of isolate forsters of D, every group has each 50 of grub, corn borer, pine caterpillars, grub, corn borer termite, locust.
A organizes every two weeks and uses the present invention to process once, B organizes and within every two weeks, uses the component of removing after blue flower principal columns of a hall branches and leaves extract and tree peony root extract in the present invention to process once, C organizes every two weeks and uses the component of removing after saccharomycete in the present invention to process once, and D group is not done any processing.
Result of the test: after three months, the number of A group grub, corn borer, pine caterpillars, termite, locust is respectively 0,2,1,1,3; The number of B group grub, corn borer, pine caterpillars, termite, locust is respectively 6,8,10,8,10; The number of C group grub, corn borer, pine caterpillars, termite, locust is respectively 1,2,2,1,4; The number of D group grub, corn borer, pine caterpillars, termite, locust is respectively 48,46,45,47.
This test explanation, a kind of effectively insecticide of biopesticide of the present invention, and played obvious promotion insecticidal action after adding blue flower principal columns of a hall branches and leaves extract and tree peony root extract.
Claims (3)
1. a biopesticide, it is characterized in that: described biopesticide is by potassium fulvate, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder, China tree seed extracts, saccharomycete, blue flower principal columns of a hall branches and leaves extract and tree peony root extract are made, the parts by weight of wherein preparing the required each raw material of biopesticide are respectively: potassium fulvate 3-4 part, Candida albicans powder 0.008-0.01 part, nitrifying bacteria powder 0.01-0.03 part, white muscardine fungi powder 0.1-0.2 part, green muscardine fungus powder 0.3-0.5 part, saccharomycete 0.01-0.03 part, China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, tree peony root extract 18-20 part.
2. the preparation method of a kind of biopesticide according to claim 1, is characterized in that, comprises the following steps:
Step 1, choose disease-free tree peony root, after shredding, putting into mortar grinds, in mortar, add absolute ether afterwards, the volume of the absolute ether that every gram of tree peony root is corresponding is 2mL, the ultrasonoscope of afterwards extract being put into frequency and be 22kHz extracts, and has extracted rear filtration and with activated carbon decolorizing, has then put into Rotary Evaporators and heat, and be under the condition of 95-105 ℃, to collect evaporation phase in temperature, obtain tree peony root extract;
Step 2, China tree seed and blue flower principal columns of a hall branches and leaves are pulverized, and proceed to respectively and in mortar, grind to form homogenate, proceed to respectively afterwards in the centrifuge that rotating speed is 10000r/min centrifugal, obtain the extract of China tree seed extracts and blue flower principal columns of a hall branches and leaves, both extracts are mixed and stirred, wherein China tree seed extracts 9-16 part, blue flower principal columns of a hall branches and leaves extract 8-13 part, obtain mixture I, for subsequent use;
Step 3, take Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder, green muscardine fungus powder and saccharomycete according to the parts by weight of each component claimed in claim 1, Candida albicans powder, nitrifying bacteria powder, white muscardine fungi powder and green muscardine fungus powder are put into same medium to be cultivated, after 6-7 days, add saccharomycete, cultivate and obtain mix bacterium agent I after 15-20 days, for subsequent use;
Step 4, take potassium fulvate and tree peony root extract according to the parts by weight of each component claimed in claim 1, afterwards will mixture I, mix bacterium agent I, potassium fulvate and tree peony root extract stir after mixing.
3. the preparation method of a kind of biopesticide according to claim 2, is characterized in that: described medium is made up of potato, sucrose and water, and wherein the parts by weight of each component are respectively: potato 19-21 part, sucrose 2-3 part, 100 parts, water.
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| CN106508991A (en) * | 2016-10-17 | 2017-03-22 | 广东盆地号生物产业有限公司 | Biological pesticide and preparation method thereof |
| CN106818911A (en) * | 2016-12-31 | 2017-06-13 | 新昌县迪斯曼科技有限公司 | Public nuisance-free agricultural chemicals containing microorganism of green and preparation method thereof |
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- 2014-01-27 CN CN201410039325.0A patent/CN103783092B/en not_active Expired - Fee Related
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| CN103351182A (en) * | 2013-06-28 | 2013-10-16 | 青岛扎西生物科技有限公司 | Microbe-containing biofertilizer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106258594A (en) * | 2016-08-25 | 2017-01-04 | 周宁县绿绿山农林综合开发有限公司 | Taxus mairei culture medium and breeding method |
| CN106508991A (en) * | 2016-10-17 | 2017-03-22 | 广东盆地号生物产业有限公司 | Biological pesticide and preparation method thereof |
| CN106818911A (en) * | 2016-12-31 | 2017-06-13 | 新昌县迪斯曼科技有限公司 | Public nuisance-free agricultural chemicals containing microorganism of green and preparation method thereof |
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| CN103783092B (en) | 2015-05-13 |
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