CN105053015B - It is a kind of to be used to prevent and treat biological pesticide of tree peony brown spot and preparation method thereof - Google Patents
It is a kind of to be used to prevent and treat biological pesticide of tree peony brown spot and preparation method thereof Download PDFInfo
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- CN105053015B CN105053015B CN201510412155.0A CN201510412155A CN105053015B CN 105053015 B CN105053015 B CN 105053015B CN 201510412155 A CN201510412155 A CN 201510412155A CN 105053015 B CN105053015 B CN 105053015B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention belongs to biological technical field, and in particular to a kind of to be used to prevent and treat biological pesticide of tree peony brown spot and preparation method thereof.The present invention has the bacillus subtilis HS5B5 of antagonism to be compounded with bio-fertilizer and Bordeaux mixture using to tree peony brown spot disease fungus tree peony branch spore is mould, it significantly more efficient can suppress the mould growth and breeding of tree peony branch spore, and then reach the purpose of preventing and treating tree peony brown spot, Bordeaux mixture also has inhibitory action to the other fungal diseases of tree peony simultaneously, by with after the compounding of bacillus subtilis and bio-fertilizer, enhancing the prevention effect to tree peony fungal disease.The present invention adds bio-fertilizer and Bordeaux mixture in bacillus subtilis HS5B5, additionally it is possible to kind of blooming period prolonging, and averagely 23 days are extended compared with general planting.The method that the present invention is prevented and treated using biological pesticide, environmentally safe to person poultry safety, high-efficiency broad spectrum is not likely to produce the resistance to the action of a drug.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of biological pesticide and its system for being used to prevent and treat tree peony brown spot
Preparation Method.
Background technology
Tree peony is the famous ornamental flower of China, grow with During Overwintering Period, easily by pathogenic microorganism
Contaminate and cause lesion.Peony disease species survey is more, and harm is serious, and most of these diseases are fungal diseases, such as brown
Pinta, gray mold, rust, powdery mildew and purple root rot (Helicobasidium mompa) etc..They can cause peony leaves local necrosis or whole leaf withered, harm
Leaf, branch and the rhizome portion of tree peony, make tree peony fall leaves in advance, influence its nutrient growth and ornamental value.Tree peony brown spot is most
For one of serious disease, main harm blade can also infect stem and flower.Occur afterwards before flowering more than disease, continue up to
The dead leaf phase.Disease fungus is that tree peony branch spore is mould（Cladosporium paeoniae）, belong to Deuteromycotina, Haplomycetes, clump stalk
Spore mesh, cladosporium category.Cause of disease is survived the winter with mycelium or conidium in dead leaf or soil, and propagating early summer by wind and rain starts hair
Raw, autumn harm is serious, and high temperature, high humidity, illumination deficiency, improper ventilation, continuous cropping etc. are conducive to disease.
The preventing and treating of tree peony brown spot primarily now relies on the use of chemicals, and this is not only to health and ecological environment
Safety hazard, and easily cause the pathogenic microorganism resistance to the action of a drug.Biological pesticide is to utilize microorganism or the control agriculture of its metabolite
Crop disease and insect or the prevention and controls for adjusting crop growth, but its research and application in the preventing and treating of tree peony fungal disease is also very
It is few.In order to strengthen the fast development of tree peony industry, develop it is harmless to health and ecological environment security, be difficult to cause cause of disease
Drug-fast biological pesticide is extremely urgent, and it is cause of disease that bacillus subtilis HS5B5 is mould to tree peony branch spore by Ant agonism
The tree peony brown spot of fungi has obvious inhibition, also have to other fungal diseases such as tree peony powdery mildew certain inhibition but
DeGrain, and bio-fertilizer and Bordeaux mixture are competed with chemical action in the suppression to tree peony powdery mildew by microbial nutrition
There is positive effect with plant growth-promoting, kind of blooming period prolonging.
The content of the invention
, can not only it is an object of the invention to provide a kind of biological pesticide for being used to prevent and treat tree peony brown spot and preparation method thereof
Enough effectively preventing tree peony brown spots, environmental sound, and can be effectively promoted growing for tree peony, kind of blooming period prolonging,
Prevent and treat some other Common fungi disease.
The technical solution used in the present invention is：A kind of biological pesticide for being used to prevent and treat tree peony brown spot, the biological agriculture
Medicine is made up of bacillus subtilis, bio-fertilizer and Bordeaux mixture, and each component parts by weight used in preparation process are respectively：It is withered
Careless bacillus 1-2 parts, 4-5 parts of 8-10 parts of bio-fertilizer and Bordeaux mixture, the bacillus subtilis is named as bacillus subtilis
Bacterium（Bacillus subtilis）HS5B5, the bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
Center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, preserving number is：CGMCC
No. 6088。
The preparation method of described biological pesticide, comprises the following steps：
Step 1: the preparation of the bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, after stirring
Fermentation in fermenting cellar is put into, is taken out after 6-7 days, mixture is made, rape cake, and mixture and rape cake are added into mixture
Mass ratio be 2:1, dried after stirring, bio-fertilizer is made；
Step 2: the preparation of the Bordeaux mixture
Copper sulphate is put into water and diluted, and the mass ratio of copper sulphate and water is 1:80, after stirring, dilution is made
Copper sulphate, quick lime is put into water, and the mass ratio of quick lime and water is 1:40, after stirring, milk of lime is made, will
Dilute sulphuric acid copper is poured into milk of lime, the stirring of side bevelling, and Bordeaux mixture is made；
It is made Step 3: being stirred after described bacillus subtilis HS5B5, bio-fertilizer and Bordeaux mixture are mixed
The parts by weight of each composition are that bacillus subtilis HS5B5 parts by weight are 1-2 parts in biological pesticide, and biological pesticide, raw
The parts by weight of thing fertilizer are 8-10 parts, and the parts by weight of Bordeaux mixture are 4-5 parts.
By the present invention in that with the Bordeaux mixture of autogamy, being deposited to the gemma of the bacillus subtilis HS5B5 in biological pesticide
In extremely low inhibitory action, survival rate is 92-95%.
Bacillus subtilis in the present invention（Bacillus subtilis）HS5B5, on May 9th, 2012 is preserved in China
Microbiological Culture Collection administration committee common micro-organisms center（CGMCC）, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number Institute of Microorganism, Academia Sinica, deposit number：CGMCC No. 6088.Saccharomycete in the present invention
（Trichosporon capitatum）Buy in the bio tech ltd of Shaanxi source one, the culture presevation is trained in Chinese Typical Representative
Thing collection is supported, preservation bacterium number is CCTCC AY 93140.Tree peony branch spore in experimentation of the present invention is mould
（Cladosporium paeoniae）Purchased from Chinese agriculture Microbiological Culture Collection administrative center, bacterial strain preserving number is ACCC
36063。
Beneficial effect
The present invention using to the tree peony brown spot disease fungus tree peony mould bacillus subtilis HS5B5 of branch spore and bio-fertilizer and
Bordeaux mixture is compounded, and significantly more efficient can suppress the mould growth and breeding of tree peony branch spore, and then reach preventing and treating tree peony brown spot
Purpose, while Bordeaux mixture has inhibitory action to tree peony powdery mildew, by with after the compounding of bacillus subtilis and bio-fertilizer, increasing
The strong prevention effect to tree peony powdery mildew.The present invention adds bio-fertilizer and Bordeaux mixture in bacillus subtilis HS5B5, also
Can kind of blooming period prolonging, compared with general planting averagely extend 2-3 days.The method that the present invention is prevented and treated using biological pesticide, to people and animals
Safety, environmentally safe, high-efficiency broad spectrum is not likely to produce the resistance to the action of a drug.
Embodiment
It is a kind of to be used to preventing and treating the biological pesticide of tree peony brown spot, the biological pesticide by bacillus subtilis, bio-fertilizer and
Bordeaux mixture is made, and each component parts by weight used in preparation process are respectively：1-2 parts of bacillus subtilis, bio-fertilizer 8-
10 parts and 4-5 parts of Bordeaux mixture, the bacillus subtilis are bacillus subtilis HS5B5.
The preparation method of described biological pesticide, comprises the following steps：
Step 1: the preparation of the bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, after stirring
Fermentation in fermenting cellar is put into, is taken out after 6-7 days, mixture is made, rape cake, and mixture and rape cake are added into mixture
Mass ratio be 2:1, dried after stirring, bio-fertilizer is made；
Step 2: the preparation of the Bordeaux mixture
Copper sulphate is put into water and diluted, and the mass ratio of copper sulphate and water is 1:80, after stirring, dilution is made
Copper sulphate, quick lime is put into water, and the mass ratio of quick lime and water is 1:40, after stirring, milk of lime is made, will
Dilute sulphuric acid copper is poured into milk of lime, the stirring of side bevelling, and Bordeaux mixture is made；
It is made Step 3: being stirred after described bacillus subtilis HS5B5, bio-fertilizer and Bordeaux mixture are mixed
The parts by weight of each composition are that bacillus subtilis HS5B5 parts by weight are 1-2 parts in biological pesticide, and biological pesticide, raw
The parts by weight of thing fertilizer are 8-10 parts, and the parts by weight of Bordeaux mixture are 4-5 parts.
Embodiment 1
It is a kind of to be used to preventing and treating the biological pesticide of tree peony brown spot, the biological pesticide by bacillus subtilis, bio-fertilizer and
Bordeaux mixture is made, and each component parts by weight used in preparation process are respectively：2 parts of bacillus subtilis, 10 parts of bio-fertilizer
With 5 parts of Bordeaux mixture, the bacillus subtilis is named as bacillus subtilis HS5B5.
The preparation method of described biological pesticide, comprises the following steps：
Step 1: the preparation of the bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, after stirring
Fermentation in fermenting cellar is put into, is taken out after 7 days, mixture is made, rape cake is added into mixture, and mixture and rape cake
Mass ratio is 2:1, dried after stirring, bio-fertilizer is made；
Step 2: the preparation of the Bordeaux mixture
Copper sulphate is put into water and diluted, and the mass ratio of copper sulphate and water is 1:80, after stirring, dilution is made
Copper sulphate, quick lime is put into water, and the mass ratio of quick lime and water is 1:40, after stirring, milk of lime is made, will
Dilute sulphuric acid copper is poured into milk of lime, the stirring of side bevelling, and Bordeaux mixture is made；
It is made Step 3: being stirred after described bacillus subtilis HS5B5, bio-fertilizer and Bordeaux mixture are mixed
The parts by weight of each composition are that bacillus subtilis HS5B5 parts by weight are 2 parts in biological pesticide, and biological pesticide, biological
The parts by weight of fertilizer are 10 parts, and the parts by weight of Bordeaux mixture are 5 parts.
Embodiment 2
It is a kind of to be used to preventing and treating the biological pesticide of tree peony brown spot, the biological pesticide by bacillus subtilis, bio-fertilizer and
Bordeaux mixture is made, and each component parts by weight used in preparation process are respectively：1 part of bacillus subtilis, 8 parts of bio-fertilizer and
4 parts of Bordeaux mixture, the bacillus subtilis is named as bacillus subtilis HS5B5.
The preparation method of described biological pesticide, comprises the following steps：
Step 1: the preparation of the bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, after stirring
Fermentation in fermenting cellar is put into, is taken out after 6 days, mixture is made, rape cake is added into mixture, and mixture and rape cake
Mass ratio is 2:1, dried after stirring, bio-fertilizer is made；
Step 2: the preparation of the Bordeaux mixture
Copper sulphate is put into water and diluted, and the mass ratio of copper sulphate and water is 1:80, after stirring, dilution is made
Copper sulphate, quick lime is put into water, and the mass ratio of quick lime and water is 1:40, after stirring, milk of lime is made, will
Dilute sulphuric acid copper is poured into milk of lime, the stirring of side bevelling, and Bordeaux mixture is made；
It is made Step 3: being stirred after described bacillus subtilis HS5B5, bio-fertilizer and Bordeaux mixture are mixed
The parts by weight of each composition are that bacillus subtilis HS5B5 parts by weight are 1 part in biological pesticide, and biological pesticide, biological
The parts by weight of fertilizer are 8 parts, and the parts by weight of Bordeaux mixture are 4 parts.
Experiment one
On April 6th, 2015,400 plants of tree peonies in the international peony garden in Luoyang Peony growing area are chosen as subjects, will be male
Pellet is divided into tetra- groups of A, B, C, D, and it is mould that the tree peony branch spore of equivalent is inoculated with to the tree peony seedling in A, B, C, D tetra-.A groups use the present invention
Biological pesticide, every two weeks sprinkling once；B groups were sprayed using the bacillus subtilis HS5B5 bacterium solutions in the present invention every two weeks
Spill once；C groups remove the biological pesticide after bacillus subtilis HS5B5 using the present invention, every sprinkling in two weeks once；D groups are every
A water was sprayed every two weeks, control is used as.
June 5 in 2015 daily inspection result：Occur in A groups without tree peony brown spot and tree peony powdery mildew, plant strain growth is healthy and strong, B
Group has 6 plants of tree peonies to occur brown spot, has 17 plants of tree peonies to occur powdery mildew, and plant is substantially healthy and strong not as A groups, 32 plants of tree peonies in C groups
Powdery mildew occurs for generation brown spot, 2 plants of tree peonies, though plant is healthy and strong substantially more healthy and stronger than B groups not as A groups, 79 plants of tree peony hairs in D groups
Raw brown spot, while powdery mildew occurs for 14 plants of tree peonies, plant strain growth is small and weak.
As a result show：Bacillus subtilis HS5B5 can prevent tree peony brown spot, while also having necessarily to tree peony powdery mildew
Inhibitory action, bio-fertilizer and Bordeaux mixture be used in mixed way and can increase soil fertility, and stimulates plant growth, promotes tree peony to supporting
The absorption divided, though the DeGrain that had certain effect to prevention tree peony brown spot and tree peony powdery mildew, and the life of the present invention
Thing agricultural chemicals significantly more efficient can not only prevent tree peony brown spot, and can effectively prevent tree peony powdery mildew, while may be used also
To promote the growth of plant.
Experiment two
300 plants of growing way identical tree peonies are chosen, tri- groups of A, B, C is divided into, wherein A groups use the biological pesticide of the present invention, B
Group is using the bio-fertilizer in the present invention, and C groups are without any composition.Observe and record growing state.
Result of the test：The A groups average whole florescence is 11 days, B groups average whole 9 days florescences, and the C groups average whole florescence is 6
My god.
As a result show：Bio-fertilizer can play a part of extending peony forescence, add bacillus subtilis HS5B5 and bohr
Many liquid form more obvious to the extension effect of peony forescence after biological pesticide of the invention.
Test one with the result for testing two to show, it is disease fungus that bacillus subtilis HS5B5, which leads to mould to tree peony branch spore,
Tree peony brown spot has obvious inhibition, also has certain inhibition to tree peony powdery mildew, bio-fertilizer and Bordeaux mixture are to male
There is positive effect on the suppression of red powdery mildew and plant growth-promoting, kind of blooming period prolonging.After passing ratio mixing, biological pesticide of the invention
The activity of tree peony brown spot and tree peony powdery mildew disease fungus can be suppressed, increase the premunition and resistance of tree peony, promote to plant
Strain growth, its effect and effect is not only the simple cumulative of bacillus subtilis, bio-fertilizer and Bordeaux mixture, each after compounding
There is obvious synergistic function between composition.
Claims (1)
1. a kind of preparation method for being used to prevent and treat the biological pesticide of tree peony brown spot, it is characterised in that comprise the following steps：
Step 1: the preparation of bio-fertilizer：Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is
500:1, fermentation in fermenting cellar is put into after stirring, is taken out after 6-7 days, mixture is made, rape cake is added into mixture,
And the mass ratio of mixture and rape cake is 2:1, dried after stirring, bio-fertilizer is made；
Step 2: the preparation of Bordeaux mixture：Copper sulphate is put into water and diluted, and the mass ratio of copper sulphate and water is 1:80,
After stirring, dilute sulphuric acid copper is made, quick lime is put into water, and the mass ratio of quick lime and water is 1:40, stirring
After uniform, milk of lime is made, dilute sulphuric acid copper is poured into milk of lime, Bordeaux mixture is made in bevelling stirring in side；
Step 3: by weight by the bio-fertilizer of 1-2 parts of HS5B5,8-10 parts of bacillus subtilis and 4-5 parts of Bordeaux
Stirred after liquid mixing and biological pesticide is made；The bacillus subtilis HS5B5 is bacillus subtilis（Bacillus subtilis）HS5B5, the bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address：
No. 3 Institute of Microorganism, Academia Sinica of institute of BeiChen West Road, Chaoyang District, BeiJing City 1, preserving number is：CGMCC No.
6088 。
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| CN106116883B (en) * | 2016-06-27 | 2019-08-02 | 河南科技大学 | A kind of preparation method of tree peony pouring root composite microbic bacterial fertilizer |
| CN108315281B (en) * | 2018-04-17 | 2020-01-10 | 山东省林业科学研究院 | Bacillus subtilis and application thereof in preventing and treating peony red spot disease |
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| CN102965320A (en) * | 2012-12-04 | 2013-03-13 | 赵斌 | Bacillus subtilis for preventing and controlling plant fungal disease and application of bacillus subtilis |
| WO2014147528A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a biopesticide |
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| CN104195061A (en) * | 2014-04-23 | 2014-12-10 | 河南科技大学 | Bacillus subtilis and application thereof |
| CN104284589A (en) * | 2012-05-07 | 2015-01-14 | 陶氏益农公司 | Use of pro-fungicides of UK-2A for control of black sigatoka |
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| CN101643710A (en) * | 2009-08-25 | 2010-02-10 | 山东汇丰源农业科技发展有限公司 | Bacillus subtilis and application thereof |
| CN104284589A (en) * | 2012-05-07 | 2015-01-14 | 陶氏益农公司 | Use of pro-fungicides of UK-2A for control of black sigatoka |
| CN102965320A (en) * | 2012-12-04 | 2013-03-13 | 赵斌 | Bacillus subtilis for preventing and controlling plant fungal disease and application of bacillus subtilis |
| WO2014147528A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a biopesticide |
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| CN104195061A (en) * | 2014-04-23 | 2014-12-10 | 河南科技大学 | Bacillus subtilis and application thereof |
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Effective date of registration: 20230601 Address after: No. 902, 9th Floor, Building 1, Hongzhi Space, Yutai Road West and Keda Street North, Huiji District, Zhengzhou City, Henan Province, 450000 Patentee after: Liufuding Agricultural Development Co.,Ltd. Address before: No. 3510, 35th Floor, Building 1, No. 500, Middle Section of Tianfu Avenue, Chengdu High tech Zone, China (Sichuan) Pilot Free Trade Zone, Chengdu City, Sichuan Province, 611833 Patentee before: Sichuan qianyiding Technology Co.,Ltd. Effective date of registration: 20230601 Address after: No. 3510, 35th Floor, Building 1, No. 500, Middle Section of Tianfu Avenue, Chengdu High tech Zone, China (Sichuan) Pilot Free Trade Zone, Chengdu City, Sichuan Province, 611833 Patentee after: Sichuan qianyiding Technology Co.,Ltd. Address before: 471000 No. 48, Xiyuan Road, Jianxi District, Henan, Luoyang Patentee before: HENAN University OF SCIENCE AND TECHNOLOGY |