CN104195061A - Bacillus subtilis and application thereof - Google Patents
Bacillus subtilis and application thereof Download PDFInfo
- Publication number
- CN104195061A CN104195061A CN201410163640.4A CN201410163640A CN104195061A CN 104195061 A CN104195061 A CN 104195061A CN 201410163640 A CN201410163640 A CN 201410163640A CN 104195061 A CN104195061 A CN 104195061A
- Authority
- CN
- China
- Prior art keywords
- subtilis
- bacillus subtilis
- hs5b5
- tree peony
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of subtilis and application thereof.
Background technology
Tree peony insect pest is less, but disease generation kind is many and harm is serious.Disease be tree peony its grow and During Overwintering Period in, be subject to the dip-dye of pathogenic micro-organism or the impact of unsuitable environmental condition, make it that a series of pathologies occur from physiological mechanism to morphological structure.That has identified out more than 20 plants in Peony disease, and great majority are fungal diseases, as brown spot, gray mold, rust, southern blight and purple root rot (Helicobasidium mompa) etc.They cause leaf local necrosis or whole leaf withered, harm tree peony leaf, branch and rhizome portion, tree peony is fallen leaves in advance, affect life.Tree peony brown spot is one of the most serious disease, and this sick main harm blade, also can infect stem and flower, how in SPRING WHEAT BEFORE AND AFTER FLOWERING, to occur, and continues up to the dead leaf phase.Pathogenic fungi be tree peony branch spore mould (
cladosporium paeoniae), belong to Deuteromycotina, Haplomycetes, Moniliales, cladosporium belongs to.Cause of disease is survived the winter in dead leaf or soil with mycelium or conidium, propagates early summer start to occur by wind and rain, and autumn, harm is serious, hot and humid, illumination is not enough, improper ventilation, continuous cropping etc. are all conducive to disease and occur.
The control of tree peony brown spot is the main use that relies on chemicals at present, and this is not only harmful to HUMAN HEALTH and ecological environment security, and easily causes cause of disease resistance.Biological control is to utilize the method for preventing and treating biological or the sick worm generation of its product control agricultural harm, because it has person poultry safety, environmentally safe, be difficult for developing immunity to drugs, high-efficiency broad spectrum, can promote the features such as plant growth, day by day be subject to people's attention, yet the research of biological control in Peony disease defence and application are also seldom.In order to accelerate the fast development of China's tree peony industry, develop harmless to HUMAN HEALTH and ecological environment security, be difficult for causing that the biocontrol agent of cause of disease resistance, high-efficiency broad spectrum is extremely urgent.
Summary of the invention
The object of this invention is to provide and a kind ofly can suppress the mould subtilis of tree peony Pathogenic Bacteria Causing Brown Blotch Disease tree peony branch spore.
The technical solution used in the present invention is: a kind of subtilis, described subtilis be subtilis (
bacillus subtilis) HS5B5, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center of No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on May 9th, 2012, and preserving number is: CGMCC No. 6088.
Described subtilis (
bacillus subtilis) HS5B5 bacterial strain bacterium colony on beef extract-peptone nutrient agar is white in color, shaft-like, surface drying has gauffer, raw in flagellum, colony diameter 0.8-2.3 μ m, Gram-positive.
Described subtilis (
bacillus subtilis) the 16S rDNA sequence of HS5B5 bacterial strain is as shown in SEQ ID NO:1.
Described subtilis (
bacillus subtilis) HS5B5 bacterial strain obtains by face-off culture method screening.
Described subtilis (
bacillus subtilis) HS5B5 bacterial strain is to the mould antagonism bacteriostatic action of tree peony Pathogenic Bacteria Causing Brown Blotch Disease tree peony branch spore.
Described subtilis (
bacillus subtilis) application of HS5B5 bacterial strain preparation in control tree peony brown spot.
Described subtilis (
bacillus subtilis) HS5B5 bacterial strain is to the application in chrysanthemum verticillium, apple mildew, oil tea leaf spoting bacteria, Chinese Rose.
The invention has the beneficial effects as follows:
1, subtilis of the present invention (
bacillus subtilis) HS5B5 has a very strong restraining effect to the pathogenic bacteria tree peony branch spore of tree peony brown spot is mould, can effectively prevent and treat tree peony brown spot.
2, subtilis of the present invention (
bacillus subtilis) HS5B5 is harmless to ecotope, can not cause the resistance of cause of disease.
3, subtilis of the present invention (
bacillus subtilis) culture condition of HS5B5 is simple, easily preserves, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
Fig. 1 be subtilis (
bacillus subtilis) inhibition zone of HS5B5;
Fig. 2 be subtilis (
bacillus subtilis) colony characteristics of HS5B5.
Embodiment
Screening and separating of the present invention subtilis (
bacillus subtilis) HS5B5, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is: CGMCC No. 6088.This bacterial strain has a very strong restraining effect to the pathogenic bacteria tree peony branch spore of tree peony brown spot is mould, can be used for the biotechnological formulation of preparation control tree peony brown spot.
One, the method for screening and separating of bacterial strain
1, the separation of genus bacillus in sample
(1) the Peony growing district, international peony garden in Luoyang, gathers tree peony root topsoil, gathers 100 samples in different location, and it is air-dry that laboratory is taken back in pack.Each sample after air-dry, after grinding, takes 10g and joins respectively in the triangular flask that 90mL sterilized water and 5 granulated glass spherees are housed from each sample, fully shakes 20～30min, and sample is mixed with sterilized water, makes sample suspension.
(2) sample suspension making being placed in to temperature is that 10min is heated in the water-bath of 80 ℃, to kill asporulate bacterium and other microorganisms, then the sample suspension of drawing 1mL under aseptic condition joins in the test tube that 9mL sterilized water is housed, and vibration mixes, and makes 10
-2sample diluting liquid.
Make successively 10 as stated above
-3, 10
-4, 10
-5, 10
-6, 10
-7diluent, draw respectively 10
-5, 10
-6, 10
-7each sample diluting liquid 0.1mL of concentration gradient is coated with on corresponding beef extract-peptone flat board, is inverted for 30 ℃ and cultivates 48h.According to obvious bacterium colonies of proterties diversity ratio such as colony characteristics picking forms, plate streaking purifying, saves backup after numbering repeatedly.
2, the screening of tree peony brown spot Antagonistic Fungi
The screening of antagonistic Bacillus adopt the pathogenic bacteria tree peony branch spore of tree peony brown spot mould (
cladosporium paeoniae) be target, method adopts face-off to cultivate sieve method.
For the tree peony branch spore that screens mould (
cladosporium paeoniae) purchased from Chinese agriculture microbial strains preservation administrative center, bacterial strain preserving number is ACCC 36063.
By the mould dull and stereotyped central authorities of PDA that are inoculated in of tree peony branch spore, to cultivate after 8d for 28 ℃, the Bacillus strain that access primary dcreening operation obtains, observes antibacterial situation after 28 ℃ of constant temperature culture 3d, measures antibacterial circle diameter, and every group of experiment repeats 3 times.According to the size of bacteriostatic diameter, choose comparatively significantly bacterial strain of fungistatic effect, be transferred to beef extract-peptone slant medium and save backup.
3, method for determining bacteria
(1) morphological specificity is observed
Morphological specificity is observed and is comprised observation two aspects to the observation of colonial morphology and bacterium individual morphology, mainly adopts the experimental technique in < < common bacteria system identification handbook > > to carry out.
1. the observation of colonial morphology
By bacterial classification streak culture 24h on flat board, to the color of its colonial morphology, bacterium colony quality, bacterium colony, colony edge, optical characteristics and whether secrete soluble pigment etc. and carry out observed and recorded.
2. the observation of individual morphology
Gramstaining: adopt the bacterial classification that cell age is 10～14h.After smear, first by Viola crystallina, just dye, then use the mordant dyeing of iodine liquid, after 95% ethanol decolorization, redye by sarranine again, washing, blots.Microscopy thalline be purple be gram-positive microorganism, thalline for red be Gram-negative bacteria.
Spore staining: after smear, first dye 15min by Victoria Green WPB, rinse through water, air-dryly redye 2min by sarranine afterwards, washing, blots.Microscopy is that gemma is green, and thalline and sporangium are blush.
The measurement of thalline size: with micro-micrometer, gramstaining smear is carried out the measurement of thalline size, each bacterial strain is measured width and the length of 10 above thalline, mean value is generally size of thalline.
(2) Physiology and biochemistry is identified
Physiology and biochemistry is identified according to the method in < < common bacteria system identification handbook > > and < < Bacillus > >, each bacterial strain is comprised to catalase reaction, aerobic test, clark and Lubsreaction, V.P reaction test, nitrate reduction test, sugar alcohol fermentation test, indoles produces test, Citrate trianion utilization test, Starch Hydrolysis test, casein hydrolysis experiment, hydrogen sulfide produces the evaluation of the physiological and biochemical properties such as test and salt tolerance test.
(3) 16S rDNA sequential analysis
By bacterial genomes, extract test kit and carry out bacterial genomes DNA extraction, as the template of PCR reaction.16S rDNA total length amplification universal primer for primer.Pcr amplification reaction system 30 μ L:PCR Preblend 15 μ L, template DNA 1 μ L, primer each 0.5 μ L, distilled water 13 μ L.PCR reaction conditions: 95 ℃ of 3min; 95 ℃ of 50s, 52 ℃ of 50s, 72 ℃ of 2min, 30 circulations; 72 ℃ are extended 10min.With PCR glue, reclaim test kit and reclaim PCR product, after 1.5% detected through gel electrophoresis, in 16 ℃, spend the night and be connected under the effect of T4 ligase enzyme with cloning vector pGM-T, and be transformed into bacillus coli DH 5 alpha competent cell.Primer and the order-checking of positive colony bacterium are completed by Shanghai Sheng Gong biotechnology limited-liability company.Utilize Blast that sequence known in the sequence of the 16S rDNA of bacterial strain and GenBank database is compared, analyze its homology.
Two, screening and separating result
1, the separation and purification of genus bacillus in sample
From sample, through diluting separation, purifying, obtain altogether 79 strain bacteriums, number respectively, preserve, for the screening of Antagonistic Fungi.With the preliminary microscopy of microscope, observe and find that wherein having 68 strains is genus bacillus, account for and obtain 86.1% of total plate count, in this explanation test, adopt before dilution separation, sample suspension is first heated to the treatment process of 10min in 80 ℃ of water-baths, can effectively kill the microorganism cells in sample, thereby obtain to greatest extent genus bacillus, be suitable for the separation of genus bacillus.
2, the screening of tree peony Pathogenic Bacteria Causing Brown Blotch Disease Antagonistic Fungi
With tree peony Pathogenic Bacteria Causing Brown Blotch Disease tree peony branch spore mould (
cladosporium paeoniae) as target, adopting face-off culture method, the fungistatic effect of take is that inhibition zone size is standard, carries out the screening of antagonistic Bacillus.Result shows in separated 68 bacillus that obtain, tree peony brown spot is had to inhibiting 11 strains that have from sample, account for 16.2% of sum, wherein antibacterial circle diameter has 1 strain more than 6mm, and antibacterial circle diameter is 6.18mm, see Fig. 1, called after HS5B5, carries out microorganism strains evaluation.
3, individuality and colonial morphology observations
Can find out, the thalli morphology of bacterial strain HS5B5 is shaft-like, Gram-positive, central spore, peritrichous, simultaneously from the shown feature such as irregular, dry, opaque of bacterium colony can tentatively infer bacterial strain HS5B5 may belong to bacillus (
bacillus).
The morphological specificity of table 1 bacterial strain HS5B5
4, physiological and biochemical property qualification result
HS5B5 is strict aerobic bacteria, clark and Lubsreaction and V.P reaction are all positive, all there is catalase, nitrate reductase, amylolytic enzyme and casein lytic enzyme, can utilize Citrate trianion as sole carbon source, all energy glucose fermentation, N.F,USP MANNITOL produce acid, all can under 10%NaCl, 7%NaCl, 5%NaCl, 2%NaCl salt concentration conditions, grow, all can under 45 ℃ of conditions, grow and can not under 65 ℃ of conditions, grow, its physiological and biochemical property is all identical with subtilis reference culture.According to the morphological specificity of bacterial strain HS5B5 and physiological and biochemical property, and with reference to < < common bacteria system identification handbook > > and < < Bacillus > >, can determine antagonistic strain HS5B5 belong to bacillus subtilis Pseudomonas (
bacillus).
Table 2 bacterial strain HS5B5 physiological and biochemical property
Note :+expression positive reaction;-expression negative reaction.
16S rDNA sequencing result
Take bacterial genomes as template, and after pcr amplification, product detects and occurs about 1500bp fluorescence band through 1.5% agarose gel electrophoresis.Check order in target area by positive colony bacteria plasmid, 16S rDNA clip size is 1470bp, and 16S rDNA sequence is as shown in SEQ ID NO:1.The sequencing results shows and known bacterial strain subtilis
bacillus subtilisl520(FJ906822.1) etc. the sibship of bacterial strain is the most approaching, and homology reaches 93%.The result of identifying in conjunction with bacterial strain individual morphology, colonial morphology and Physiology and biochemistry, can determine bacterial strain HS5B5 be subtilis (
bacillus subtilis).
Three, the restraining effect of bacterial strain HS5B5 to tree peony brown spot
On May 20th, 2013, choose the 200 strain tree peonies in Peony growing district, international peony garden, Luoyang as subjects, tree peony is divided into two groups of A, B, and mould to the tree peony branch spore of the tree peony seedling inoculation equivalent in two groups of A, B, afterwards A group use subtilis (
bacillus subtilis) HS5B5 bacterium liquid, every two weeks, spray once; B group was sprayed a water every two weeks.
July 20 in 2013, daily inspection result: A group only had 4 strain tree peony foxiness diease occurrences to become, and B group has 92 strain tree peony foxiness diease occurrences to become.
Result shows: the subtilis in the present invention (
bacillus subtilis) HS5B5 can effectively suppress the generation of tree peony brown spot, inhibiting rate reaches 91.67%.
Four, subtilis (
bacillus subtilis) antagonistic action of HS5B5 to multiple pathogenic bacteria
Adopt face-off culture method, the punch tool that is 6mm with diameter is got bacterium piece at the colony edge of cultured chrysanthemum verticillium, apple mildew, oil tea leaf spoting bacteria, Chinese Rose respectively, bacterium piece is inoculated into PDA dull and stereotyped central, then subtilis (
bacillus subtilis) HS5B5 is inoculated into the dull and stereotyped central equidistant position of distance, does 3 repetition, the thermostat container that is placed in afterwards temperature and is 28 ℃ is cultivated, calculating pathogenic bacteria growth inhibition ratio.Result is as table 3.
Table 3 subtilis (
bacillus subtilis) restraining effect of HS5B5 to multiple pathogenic bacteria
Result shows: the subtilis in the present invention (
bacillus subtilis) HS5B5 can effectively suppress chrysanthemum verticillium, apple mildew, oil tea leaf spoting bacteria, Chinese Rose.
SEQUENCE LISTING
<110> University Of Science and Technology Of He'nan
<120> subtilis and application thereof
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1470
<212> DNA
<213> Bacillus subtilis
<400> 1
ttgagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat gcaagtcgag 60
cggacagatg ggagcttgct ccctgatgtt agcggcggac gggtgagtaa cacgtgggta 120
acctgcctgt aagactggga taactccggg attccggcgc taataccgga tggttgtttg 180
aaccgcatgc ttcaaacata aaaggtggct tcggctacca cttacagatg gacccgcggc 240
gcattagcta ctgacgctga tatcaccacg ccaaggcaac gatgcgtagc cgacctgaga 300
gggtgatcgg cgacacaggg actgagacag ggcccagact cctacgggag gctagagcag 360
gcattcttcc gcaatgtagc aatgtctgac ggagcaacgc cgcgtgagtg atgaaggttt 420
tcggatcgta aagctctgtt gttagggaag atcaagtacc gttcgaatag ggcggtacgt 480
tgacggtacc taaccagtta gccacggcta actacgtgcc agctgccgcg gtaatacgta 540
ggtggcaagc gttgtccgga cacgtggacg gtaaagggct cgcaggcggt ttcttaagtc 600
tgatgtgaaa gcccccggct caaccgccga gggtcattgg aaactcggga acttgagtgc 660
agaagaggag agtggaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaacac 720
cagtggcgaa ggcgactctg tggtctgtaa ctgacgctga ggagcgaaag cgtggggagc 780
gaacaggatt agataccctg gtagtacggc tgagtgctaa gtgttagggg ccgtaaacga 840
gtttccgccc cttagtgctg cagctaacgc attaagcact ccgcctgggg agtacggtcg 900
caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 960
attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctctgacaat cctagagata 1020
ggacgtcccc gagtgacagg tggtgcatgg ggtggcaagc ctcgtgtcgt ttcgggggca 1080
gagatgttgg gttatgtccc cagccgccga caacccttga tcttagttgc cagcattcag 1140
ttgggcactc taaggtgact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1200
catcatgccc cttatgacct ggcctacaca cgtgctacaa tggacagaac aaagggcagc 1260
gaaaccgcga ggttaagcca atcccacaaa tctgttctca gttcggatcg cagtctgcaa 1320
ctcgactgcg tgatgctgga atcgctagta atcgcgcatc agcatgccgc ggtgaatacg 1380
ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1440
tgaggtaacc ttttaggagc cagccgccga 1470
Claims (4)
1. a subtilis, is characterized in that: described subtilis be subtilis (
bacillus subtilis) HS5B5, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is: CGMCC No. 6088.
2. the application of a kind of subtilis as claimed in claim 1 in inhibition tree peony Pathogenic Bacteria Causing Brown Blotch Disease tree peony branch spore is mould.
3. a kind of subtilis as claimed in claim 1 is in the application for the preparation of in control tree peony brown spot preparation.
4. the application of a kind of subtilis as claimed in claim 1 in suppressing chrysanthemum verticillium, apple mildew, oil tea leaf spoting bacteria, Chinese Rose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410163640.4A CN104195061B (en) | 2014-04-23 | 2014-04-23 | Bacillus subtilis and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410163640.4A CN104195061B (en) | 2014-04-23 | 2014-04-23 | Bacillus subtilis and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104195061A true CN104195061A (en) | 2014-12-10 |
| CN104195061B CN104195061B (en) | 2017-01-18 |
Family
ID=52080431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410163640.4A Active CN104195061B (en) | 2014-04-23 | 2014-04-23 | Bacillus subtilis and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104195061B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053015A (en) * | 2015-07-15 | 2015-11-18 | 河南科技大学 | Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide |
| CN105053064A (en) * | 2015-07-22 | 2015-11-18 | 河南科技大学 | Nuisanceless biological compound pesticide and preparation method thereof |
| CN106942276A (en) * | 2017-03-16 | 2017-07-14 | 河北省农林科学院植物保护研究所 | Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew |
| CN107384436A (en) * | 2017-09-13 | 2017-11-24 | 河南科技大学 | A kind of vega soil conditioner and preparation method and application |
| CN108689770A (en) * | 2018-08-27 | 2018-10-23 | 河南科技大学 | One grows tobacco pouring root bio-fertilizer and preparation method thereof and application method |
| CN108913622A (en) * | 2018-07-06 | 2018-11-30 | 四川农业大学 | The preparation and application of a kind of bacillus megaterium BM22 and its gemma pulvis |
| CN109536425A (en) * | 2019-01-24 | 2019-03-29 | 河南科技大学 | A kind of Te Jila bacillus and its application |
| CN111316998A (en) * | 2020-03-03 | 2020-06-23 | 胡赞民 | Application of bacillus subtilis HF1 in planting rosaceous plants |
| CN114128725A (en) * | 2021-12-16 | 2022-03-04 | 四川省自然资源科学研究院 | Bacillus subtilis KT-10 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008017A (en) * | 2006-01-23 | 2007-08-01 | 徐文超 | Bacillus subtilis (Ehrenberg)Cohn fermented product and its production method and uses |
| CN101463339A (en) * | 2009-01-08 | 2009-06-24 | 中国农业大学 | Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof |
| WO2013178655A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the lipid membrane synthesis, the melanine biosynthesis, the nucleic acid synthesis or the signal transduction |
-
2014
- 2014-04-23 CN CN201410163640.4A patent/CN104195061B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008017A (en) * | 2006-01-23 | 2007-08-01 | 徐文超 | Bacillus subtilis (Ehrenberg)Cohn fermented product and its production method and uses |
| CN101463339A (en) * | 2009-01-08 | 2009-06-24 | 中国农业大学 | Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof |
| WO2013178655A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the lipid membrane synthesis, the melanine biosynthesis, the nucleic acid synthesis or the signal transduction |
Non-Patent Citations (4)
| Title |
|---|
| 于生: "枯草芽孢杆菌防治甜菜褐斑病田间试验", 《中国糖料》 * |
| 张兴华 等: "枯草芽孢杆菌防治棉黄萎病研究", 《江西农业学报》 * |
| 段瑛瑜: "菏泽地区牡丹病害调查和控制技术研究", 《中国优秀硕士学位论文全文数据库·农业科技辑》 * |
| 牛晓庆 等: "油茶叶枯病菌的鉴定及生物学特性研究", 《热带作物学报》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053015B (en) * | 2015-07-15 | 2017-10-27 | 河南科技大学 | It is a kind of to be used to prevent and treat biological pesticide of tree peony brown spot and preparation method thereof |
| CN105053015A (en) * | 2015-07-15 | 2015-11-18 | 河南科技大学 | Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide |
| CN105053064A (en) * | 2015-07-22 | 2015-11-18 | 河南科技大学 | Nuisanceless biological compound pesticide and preparation method thereof |
| CN105053064B (en) * | 2015-07-22 | 2017-10-10 | 河南科技大学 | A kind of harmless boilogical composite pesticide and preparation method thereof |
| CN106942276A (en) * | 2017-03-16 | 2017-07-14 | 河北省农林科学院植物保护研究所 | Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew |
| CN107384436B (en) * | 2017-09-13 | 2021-01-29 | 河南科技大学 | Soil conditioner for tobacco field and preparation method and application thereof |
| CN107384436A (en) * | 2017-09-13 | 2017-11-24 | 河南科技大学 | A kind of vega soil conditioner and preparation method and application |
| CN108913622A (en) * | 2018-07-06 | 2018-11-30 | 四川农业大学 | The preparation and application of a kind of bacillus megaterium BM22 and its gemma pulvis |
| CN108689770A (en) * | 2018-08-27 | 2018-10-23 | 河南科技大学 | One grows tobacco pouring root bio-fertilizer and preparation method thereof and application method |
| CN109536425A (en) * | 2019-01-24 | 2019-03-29 | 河南科技大学 | A kind of Te Jila bacillus and its application |
| CN111316998A (en) * | 2020-03-03 | 2020-06-23 | 胡赞民 | Application of bacillus subtilis HF1 in planting rosaceous plants |
| CN111316998B (en) * | 2020-03-03 | 2021-10-01 | 胡赞民 | Application of bacillus subtilis HF1 in planting rosaceous plants |
| CN114128725A (en) * | 2021-12-16 | 2022-03-04 | 四川省自然资源科学研究院 | Bacillus subtilis KT-10 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104195061B (en) | 2017-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104195061A (en) | Bacillus subtilis and application thereof | |
| CN103695356B (en) | A kind of bacillus amyloliquefaciens and microbiobacterial agent and application thereof | |
| CN102485880B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN103451128B (en) | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease | |
| CN102286412B (en) | Bacillus velezensis and application thereof in tomato blight antagonism | |
| CN107099474B (en) | Pseudomonas chlororaphis with broad-spectrum antibacterial activity and application thereof | |
| CN105543132B (en) | A kind of Methylotrophic bacillus YB-F7 and its application in controlling plant diseases | |
| CN102533593A (en) | Burkholderia cepacia SD7 and culturing method and application thereof | |
| CN103756931A (en) | Paenibacillus kribbensis and its application | |
| CN101148649B (en) | Bacillus firmus, bacterium agent and application thereof | |
| CN107267412B (en) | Bacillus methylotrophicus and application thereof | |
| CN103436457A (en) | Burkholderia cepacia, and cultivation method and application thereof | |
| CN108893424B (en) | Bacillus megaterium BM22, and microcapsule preparation and application thereof | |
| CN109207404A (en) | Siam bacillus YJ15 and its application | |
| CN112899201A (en) | Bacillus belgii, application thereof and method for preventing and treating banana wilt | |
| CN103540542A (en) | Bidirectional burkholderia as well as culture method and application thereof | |
| CN101812407B (en) | Curvularia lunata strain and application thereof | |
| CN113846039B (en) | Bacillus bailii and application thereof | |
| CN106148232A (en) | The bacterial isolates of one strain antagonism plant pathogenetic bacteria and application thereof | |
| CN108913622B (en) | Bacillus megaterium BM22 and preparation and application of spore powder thereof | |
| CN102747005B (en) | Bacillus atrophaeus for prevention and control of cotton boll blight, and microbial agent thereof | |
| CN102978130B (en) | Bacillus pumilus GBSC66 and its application | |
| CN107760630B (en) | Bacillus methylotrophicus B18, microbial inoculum and application thereof | |
| CN102604869B (en) | Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof | |
| CN105524841A (en) | Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191008 Address after: Room 3b-5008, Agricultural University International Business Park, No. 10, Tianxiu Road, Haidian District, Beijing Patentee after: Yidi international agriculture (Beijing) Co., Ltd. Address before: 471000 Xiyuan Road, Jianxi District, Henan, No. 48, No. Patentee before: Henan University of Science and Technology |
|
| TR01 | Transfer of patent right |