CN105543132B - A kind of Methylotrophic bacillus YB-F7 and its application in controlling plant diseases - Google Patents
A kind of Methylotrophic bacillus YB-F7 and its application in controlling plant diseases Download PDFInfo
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Abstract
The invention discloses a kind of Methylotrophic bacillus YB-F7 and its applications in controlling plant diseases.YB-F7 bacterial strain of the present invention is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.10834；The nucleotides sequence of the bacterial strain is classified as SEQ ID NO.1.It is one plant of microbial bacterial agent having to gaeumannomyces graminis, wheat sharp eyespot and fusarium graminearum soil-borne disease compared with high inhibition that the present invention, which screens the Methylotrophic bacillus YB-F7 isolated, YB-F7 microbial inoculum of the present invention can be used as crop sowing time or disease originates phase application, apply crop rhizosphere or spraying application by pouring root after being dressed seed or be diluted with water using fermentation preparation and seed；It can be used in mixed way with to this biocontrol bacterial strain other fungicide without side-effects, plant modifying agent, adjust edaphon flora, control wheat soil-borne diseases achieve the purpose that ecological efficient.
Description
One, technical field:
The present invention relates to plant disease technical fields, and in particular to a kind of Methylotrophic bacillus YB-F7 with
And it is acted on using the bacillus controlling plant diseases and is preparing the application in controlling plant diseases microbial inoculum.
Two, technical background:
Currently, microbial control technology is to modernize the important component of Agricultural Sustainable Development, due to chemical pesticide
Use, lead to nature long-term accumulation harmful substance, cause the serious consequences such as environmental pollution, disruption of ecological balance, with to micro- life
Object pesticide this useful environment, Study of Prevention Technology that is pollution-free, maintaining the ecological balance, have become instead of the use of chemical pesticide
The hot spot of the research of microbial control at present.
Bacillus is a kind of gram-positive bacteria, has rapid growth, character stabilization, adaptability and resistance strong,
In the application of crop field, more other biocontrol microorganisms are with the obvious advantage, while, no pollution to the environment nontoxic to people and animals；Bacillus metabolite
It is abundant, be secreted into it is extracellular can inhibit, kill pathogen, or change pathogen environment inhibits its growth；Some of bacterial strains can be with
A variety of antibacterial materials are generated, these antibacterial materials play key effect during biological and ecological methods to prevent plant disease, pests, and erosion.Bacillus is a kind of common life
Fungi-proofing, there are many bacillus reports with functions such as antagonism, growth-promotings at present.Therefore, screening is efficiently and activity stabilized
Antagonistic strain produces efficient bacteria agent, promotes the research of novel microbial pesticide, ensures China's agricultural sustainable development tool
It is significant.
Three, summary of the invention:
The technical problem to be solved by the present invention is providing a kind of Methylotrophic bacillus (Bacillus
Methylotrophicus) YB-F7 and its application in controlling plant diseases.Methylotrophic bacillus of the present invention
Application of (Bacillus methylotrophicus) YB-F7 in controlling plant diseases is particularly for complete in biological control
Lose axis caused by plant roots stem disease caused by germ plant soil-borne diseases, sheath blight fungus or root-rot sickle-like bacteria
Application in basal stem rot evil；Caused by can also be used to plant soil-borne diseases, sheath blight fungus caused by preparation prevention and treatment Pathogen of Take-all
Application in the evil microbial inoculum of plant base rot disease caused by plant roots stem disease or root-rot sickle-like bacteria.
The present invention solves the above problems by the following technical programs:
The present invention provides a kind of Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7, the bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.10834.
The preservation information of Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 is as follows:
Classification naming: Methylotrophic bacillus；
Depositary institution: China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC)；
Preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica；
Preservation date: on May 21st, 2015；
Deposit number: CGMCC NO.10834.
According to above-mentioned Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7, the methyl
The nucleotides sequence of auxotype bacillus YB-F7 is classified as SEQ ID NO.1.
Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 of the present invention is used for anti-in biology
It controls plant soil-borne diseases caused by Pathogen of Take-all, planted caused by plant roots stem disease caused by sheath blight fungus or root-rot sickle-like bacteria
Application in object brown foot rot disease.
Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 of the present invention is used to prepare prevention and treatment
Plant caused by plant soil-borne diseases caused by Pathogen of Take-all, plant roots stem disease caused by sheath blight fungus or root-rot sickle-like bacteria
Application in brown foot rot disease microbial inoculum.
Positive beneficial effect of the invention:
Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 for being filtered out of the present invention is
One plant to gaeumannomyces graminis (Gaeumannomyces graminis), rhizoctonia cerealis (Rhizoctonia
Cerealis), root-rot sickle-like bacteria (Fusarium graminearum) soil-borne disease has the microbial bacterial agent compared with high inhibition,
YB-F7 bacterial strain is respectively 90%, 85.5% to gaeumannomyces graminis, rhizoctonia cerealis and root-rot sickle-like bacteria indoor inhibitory effect
With 79.8%；Greenhouse pot culture prevention and treatment take-all, wheat sharp eyespot effect respectively reach 65.7%, 76.5%, and preventive effect is higher than
Comparison medicament is suitable with comparison medicament.YB-F7 bacterial strain of the present invention is by isolated in wheat rhizosphere soil, to crops
Safety；YB-F7 microbial inoculum of the present invention can be used as crop sowing time or disease originates phase application, dressed seed using fermentation preparation and seed or
Crop rhizosphere or spraying application are applied by pouring root after being diluted with water；And it is possible to without side-effects its of this biocontrol bacterial strain
Its fungicide, plant modifying agent are used in mixed way, and adjust edaphon flora, and control wheat soil-borne diseases reach ecological efficient
Purpose.
Four, Detailed description of the invention:
Fungistatic effect figure of Fig. 1 YB-F7 bacterial strain of the present invention to gaeumannomyces graminis；
Fungistatic effect figure of Fig. 2 YB-F7 bacterial strain of the present invention to rhizoctonia cerealis；
Fungistatic effect figure of Fig. 3 YB-F7 bacterial strain of the present invention to Fusarium root rot of wheat；
Fig. 4 biocontrol microorganisms YB-F7 prevention and treatment wheat sharp eyespot control efficacy of the present invention measures schematic diagram；
Fig. 5 biocontrol microorganisms YB-F7 of the present invention prevents and treats take-all pot experiment schematic diagram；
Fig. 6 YB-F7 bacterial strain 16rDNA sequential system development tree schematic diagram of the present invention.
Five, specific embodiment:
The present invention is further explained with reference to embodiments, but is not intended to limit the contents of the present invention.
Embodiment 1: Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 bacterial strain of the present invention
Screening with separate:
For trying disease fungus: gaeumannomyces graminis (Gaeumannomyces graminis), rhizoctonia cerealis
(Rhizoctonia cerealis) and root-rot sickle-like bacteria (Fusarium graminearum).
Bacteria culture media:
NA culture medium: beef extract 3g, yeast extract 1g, peptone 5g, glucose 10g, agar 15g, water 1L, pH 6.8~
7.0,121 DEG C of high pressure sterilization 30min.
LB liquid medium: peptone 10g, yeast extract 7.0,121 DEG C of high pressure sterilizations of 5g, NaCl 10g, water 1L, pH
30min。
The isolation and purification of Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 of the present invention:
The applicant in Henan Academy of Agricultural Sciences's Zhumadian Xiping County village Yu Ying experimental field planting base, in 2010~
Progress wheat root disease separation in 2014 and the variation investigation of rhizospheric microflora of soil diversity, it is right in this development test
The separation of antagonistic strain plate screening.Soil collection is the earthing for removing root surface about 5cm from wheat rhizosphere soil, by S-shaped, with
Machine acquires the loose earth that plant root combines, or integrally digs out from soil, shakes off mixed soil sample using local method is trembled, will be each
3 repetition soil samples of place acquisition are dried in the shade.It weighs the soil sample that 1g dries in the shade to be fitted into sterilizing test tubes, 9mL sterile water is added, is being vortexed
The soil dilution liquid of 1:10 is made in sufficient vortex 1-2min on instrument.1mL is taken out from gained soil dilution liquid, and it is sterile that 9mL is added
Water sufficient vortex at 1:10 soil dilution liquid, and so on, 1:10 is made3、1:104、1:105Soil dilution liquid, respectively take
200 μ L soil dilution liquid make bacterium solution uniformly be distributed in NA planar surface for bacterium solution is spreadable among plate, with spreading rod, are placed in
In 28 DEG C of constant incubator, culture is about for 24 hours.The single colonie of picking difference colonial morphology, by being drawn on NA plate after microscopy
Line, 28 DEG C of purifying culture, it is spare to get arriving that the bacterial strain that significant changes do not occur for bacterium colony growth characteristics is stored in NA medium slant
The Antagonistic Fungi isolated and purified.
Antagonistic Fungi screening:
By the above-mentioned Antagonistic Fungi isolated and purified with disease fungus (i.e. gaeumannomyces graminis, rhizoctonia cerealis and root
Rotten sickle-like bacteria) it is target, using plate opposite culture method, the stem of diameter 5mm is inoculated in PDA plate right-angled intersection center
Place, 25 DEG C of culture carton upside down culture 1-2d connect 4 kinds of different bacteriums, after 25 DEG C of culture 2-3d, observe its fungistatic effect, and measure
Antibacterial band size selects the preferable bacterial strain of fungistatic effect to carry out secondary screening to get the preferable primary dcreening operation antagonistic strain of fungistatic effect has been arrived.
Antagonistic strain secondary screening:
Fermentation liquid preparation: the antagonistic strain that above-mentioned primary dcreening operation obtains is seeded in LB liquid medium, 30 DEG C of shaking table cultures
- 48h for 24 hours takes supernatant spare.
Bacteria culturing: being that this laboratory saves for examination pathogen, aseptically, oblique from cultured pathogen
It is forwarded on PDA plate, cultivates 5-7 days on face, it is spare.
By the disease fungus of above-mentioned spare culture to full ware, stem is beaten with 5mm punch, access PDA plate cross is handed over
Centre is pitched, 1-2d is inverted in 25 DEG C of incubator cultures, and after taking-up at the central surrounding position 3cm of distance, sterile working is being cultivated
Primary surface vertically puts Oxford cup (internal diameter 6mm, high 10mm) and gently presses culture medium, so that it is contacted tight with culture medium, in Oxford
100uL fermentation liquid (fermentation liquid of i.e. above-mentioned preparation) is added in cup, pays attention to not overflowing fermentation liquid, 25 DEG C of incubator culture 2-3d
Afterwards, fungistatic effect is observed, bacteriostatic diameter is measured.Finally methyl of the present invention is named as by one plant of obtained strongest bacteriostatic effect to seek
Support type bacillus YB-F7 bacterial strain.
Two diameter average values-bacteria cake diameter of colony diameter (mm)=perpendicular
The identification of Methylotrophic bacillus YB-F7 bacterial strain of the present invention:
The bacterial strain YB-F7 separated to the present invention carries out further Morphological Identification by Observation of biological characteristics, side
Method is as follows:
(1) it dyes: being dyed by Gram-staining process；
(2) form and cultural characteristic: 28 DEG C of culture 48h on LB solid medium observe its colony characteristics and color etc.,
Thallus feature is observed with optical microscopy；
LB solid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, agar 15g add water to 1000mL；
(3) it physiological and biochemical property: is carried out referring to " common bacteria system identification handbook " method；
(4) 16S rDNA and analysis: bacterial strain YB-F7 is using LB liquid medium in 30 DEG C of shaken cultivations to logarithmic growth
Phase is centrifuged with 10000r/min, collects thallus, conventionally extracts bacterial genomes DNA, is synthesized for expanding bacterium
The general PCR primer of 16S rDNA sequence is expanded, and the amplified production of length 1.4kb is obtained, and product is carried out recycling and is gone forward side by side
Row sequencing, analyzes sequencing result by BLAST, carries out tetraploid rice with the sequence in GenBank database, determines
The classification position of the bacterial strain.
LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g add water to 1000mL；
The above experimental result record is as follows:
1) bacterial strain YB-F7 morphological feature:
Bacterial strain YB-F7 round or ellipse, milky, dimpling, neat in edge, individual cells size on LB culture medium
(0.6~0.7) × (2~3) micron.
2) see Table 1 for details for bio-chemical characteristics result:
The bacterial strain YB-F7 biocontrol microorganisms physicochemical characteristics of the present invention of table 1
| Pilot project | Test result | Pilot project | Test result |
| Gram's staining | + | Urase | - |
| Starch Hydrolysis | + | Lipase | + |
| Gelatin hydrolysis | + | Indoles | + |
| Anaerobic growth | + | Hydrogen sulfide | - |
| Methyl red | - | Glucose | + |
| V-P experiment | + | Maltose | + |
| Citrate utilizes | + | Arabinose | + |
| Catalase | + | Xylose | + |
| Oxidizing ferment | + | Galactolipin | + |
| Nitrate reduction | + | Mannitol | + |
3) Molecular Identification of Methylotrophic bacillus YB-F7 of the present invention:
Methylotrophic bacillus molecular biology identification of the present invention: being template with drawing using bacterial strain YB-F7 genomic DNA
Object 16srDNA carries out PCR amplification, and it is 1443bp that amplification, which hands over sequencing company to measure the strain sequence length, and complete sequence is submitted
GenBank is searched for using BLAST homology, is carried out similarity analysis with strain sequence each in database, is searched at first 100
As a result in, 81 are B.methylotrophicus bacterial strain, and consistency is up to 99% or more.By bacterial strain YB-F7's
The sequence and B.methylotrophicus of the visible bacterial strain YB-F7 of phylogenetic tree of 16SrDNA sequence analysis is polymerized to one kind, single
A branch is solely constituted, genetic distance is most short, and tree branch reliability reaches 100%, in conjunction with physiological and biochemical property, thus identifies this hair
Bright YB-F7 bacterial strain is Methylotrophic bacillus methylotrophicus.Separated bacterial strain YB-F7 base
Because a group 16rDNA sequence is detailed in SEQ ID NO.1 (YB-F7 bacterial strain 16rDNA sequential system development tree is detailed in attached drawing 6).
Embodiment 3: biocontrol microorganisms YB-F7 of the present invention is to gaeumannomyces graminis, sheath blight fungus and root-rot sickle-like bacteria Indoor antibacterial
Effect experiment:
The present invention chooses representational soil using three kinds of face-off, Oxford cup and mycelial growth rate method methods in ware
Earth propagates disease, rhizome portion disease and brown foot rot disease, determine YB-F7 bacterial strain of the present invention to Pathogen of Take-all, sheath blight fungus with
And the fungistatic effect of root-rot sickle-like bacteria.Test result is as shown in table 2.In addition it is detailed in attached drawing 1,2,3, attached drawing 1,2,3 is respectively this
Invention biocontrol microorganisms YB-F7 shows figure to the inhibitory effect of Pathogen of Take-all, sheath blight fungus and root-rot sickle-like bacteria.
The YB-F7 bacterial strain of the present invention of table 2 is to three kinds of disease fungus fungistatic effects
| Pathogen | Gaeumannomyces graminis | Rhizoctonia cerealis | Root-rot sickle-like bacteria |
| Antibacterial band (mm) | 13 | 11 | 6 |
| Inhibiting rate (%) | 90.0 | 85.5 | 79.8 |
By table 2 and attached drawing 1,2,3 it can be seen that YB-F7 bacterial strain of the present invention is to gaeumannomyces graminis, sheath blight fungus and root
Rotten sickle-like bacteria has preferable inhibitory effect；The raw survey experiment of viable bacteria and without fermented liquid shows the bacterial strain to Pathogen of Take-all, line
Blight bacterium and root-rot sickle-like bacteria inhibitory effect are obvious.So Methylotrophic bacillus (Bacillus of the present invention
Methylotrophicus) YB-F7 can be effectively used for the plant soil-borne diseases caused by biological control Pathogen of Take-all, sheath blight fungus
Application in the evil of plant base rot disease caused by caused plant roots stem disease or root-rot sickle-like bacteria.
Embodiment 4: biocontrol microorganisms YB-F7 prevention and treatment wheat sharp eyespot control efficacy measurement (referring to attached drawing 4) of the present invention:
(1) experimental material:
1) for trying wheat breed: Zheng wheat 9023；
2) reagent agent: 20% jinggangmeisu WP；
3) strains tested: rhizoctonia cerealis and biocontrol bacterial strain of the present invention are Plant Protection Inst., Henan Agricultural Science Academy
Molecular components from.
(2) experimental design:
1) biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid culture: biocontrol bacterial strain of the present invention is seeded to LB liquid medium (with above-mentioned), and 37 DEG C turn
Fast 180r/min, culture 48h are spare, and (what culture obtained herein is biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid stoste, and also referred to as antagonistic strain ferments
Liquid)；
2) prepared by rhizoctonia cerealis plate: purified rhizoctonia cerealis is inoculated with PDA plate, PDA plate culture
Base falls 20mL, about 7d is cultivated, until bacterium colony is paved with plate；
3) seed treatment: by wheat seed after 1% hypochlorite disinfectant handles 10min, then with aseptic water washing 3
It is secondary, in 25 DEG C of insulating boxs vernalization to showing money or valuables one carries unintentionally, be then soaked in the biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid of the above-mentioned preparation of the present invention (spore concentration:
109Cfu/ml), 3h is impregnated, is then seeded on the plate of sheath blight fungus, every processing 30 is repeated 4 times, after 25 DEG C of cultures for 24 hours
Cover sterile quartz sand；Blank control and medicament control are set respectively；Each processing is placed in 18-20 DEG C of illumination box, illumination
12h, it is daily normally to add water moisturizing；
4) it investigates: after emergence, observing incidence and wheat seedling growth situation, investigate emergence rate, wait the control state of an illness relatively steady
It is fixed, the state of an illness is investigated, severity and disease incidence are recorded, calculates disease index and control efficiency；
5) wheat sharp eyespot grade scale: 0 grade: complete stool is disease-free；1 grade: outer 1/2 or less browning of leaf sheath；2 grades: outer leaf sheath 1/2
The above browning；3 grades: basal part of stem generates obvious eye scab；4 grades: plant Died Of Disease.
6) preventive effect calculates:
(3) experiment process:
Processing 1: blank control, sterile water seed soaking；
Processing 2: medicament control, the seed soaking of 20% 1000 times of jinggangmeisu liquid；
Processing 3: biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid stoste seed soaking of the present invention；
Processing 4: biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid of the present invention dilutes 5 times of seed soaking；
Processing 5: biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid of the present invention dilutes 10 times of seed soaking.
(4) test result (see Table 3 for details):
The biocontrol microorganisms of the present invention of table 3 prevent and treat wheat sharp eyespot control efficacy measurement result
| Processing | Emergence rate (%) | Disease incidence (%) | Disease index (%) | Control efficiency (%) |
| Blank control | 85.8 | 90.6 | 52.8 | — |
| 20% jinggangmeisu | 90.9 | 100 | 40.9 | 22.5 |
| Carbendazim | 85 | 78.3 | 48.4 | 8.3 |
| YB-F7 stoste | 85 | 64.9 | 30.1 | 43.0 |
| YB-F7 dilution 5 | 84.2 | 76.3 | 18.1 | 65.7 |
| YB-F7 dilution 10 | 91.7 | 70.2 | 35.3 | 33.1 |
By 3 measurement result of table it can be seen that biocontrol microorganisms YB-F7 of the present invention has preferable control efficiency to wheat sharp eyespot.
Control efficacy measurement result shows that fermentation liquid dilutes 5 times of control efficiency 65.7%, is higher than 20% jinggangmeisu and carbendazim,
Control efficiency difference 43% and 33.1% after 10 times of stoste and dilution.And bacterium solution does not influence emergence rate.
Embodiment 5: biocontrol microorganisms YB-F7 of the present invention prevents and treats gaeumannomyces graminis pot experiment:
(1) test method: potting efficiency test carries out in Henan Academy of Agricultural Sciences greenhouse, by wheat seed through 1%
Hypochlorite disinfectant handle 10min after, then with aseptic water washing 3 times, vernalization is spare to showing money or valuables one carries unintentionally in 25 DEG C of insulating boxs.Using bacterium
The inoculation of cake method, places the total eclipse bacterium bacteria cake that 12 pieces of diameters are 6.0mm in each small basin equipped with sterilized soil, and that treated is small by general
Wheat Seeds are placed in above Pathogen of Take-all bacteria cake and (put one grained wheat seed on every ferfas cake), and each processing group is arranged 4 basins and repeats.
When emergence is to height of seedling 5-8cm, root irrigation is carried out.After one month, wheat seedling is extracted with root, root is rinsed with clear water and is done
Only, it and observes statistics and cures the disease effect (pot experiment schematic diagram is detailed in attached drawing 5, and see Table 4 for details for statistical result).
(2) it handles:
The antagonistic strain fermentation liquid stoste used in following treatment process is fermented for the biocontrol microorganisms cultivated in embodiment 4
Liquid stoste.
Processing 1: seed soaking.Antagonistic strain fermentation liquid stoste seed soaking 3h；
Processing 2: seed soaking.Antagonistic strain fermentation liquid dilutes 5 times of seed soaking 3h；
Processing 3: seed soaking.Antagonistic strain fermentation liquid dilutes 10 times of seed soaking 3h；
Processing 4: root irrigation.Antagonistic strain fermentation liquid stoste pouring root；
Processing 5: root irrigation.Antagonistic strain fermentation liquid dilutes 5 times of pouring roots；
Processing 6: root irrigation.Antagonistic strain fermentation liquid dilutes 10 times of pouring roots；
Processing 7: clear water control；
Processing 8: medicament control；The seed dressing of 12.5% Silthiopham.
(3) grade scale and drug effect calculation method:
The specific grade scale of take-all are as follows:
0 grade: disease-free；
1 grade: root system onset area accounts for 1%~5%；
3 grades: root system onset area accounts for 6%~20%；
5 grades: root system onset area accounts for 21%~40%；
7 grades: root system onset area accounts for 41%~60%；
9 grades: root system onset area accounts for 61% or more.
4 biocontrol microorganisms YB-F7 of table prevents and treats take-all results from pot experiment test
| Processing | Emergence rate (%) | Disease incidence (%) | Disease index (%) | Control efficiency (%) |
| Seed soaking stoste | 90.8 | 26 | 26 | 24 |
| It soaks seed 5 times of stostes | 90 | 15 | 9 | 73.5 |
| It soaks seed 10 times of stoste liquid | 86.7 | 32 | 27 | 21 |
| Stoste pouring root | 94.2 | 39 | 34 | 0 |
| 5 times of pouring roots of stoste | 79.2 | 13 | 8 | 76.5 |
| 10 times of pouring roots of stoste | 76.7 | 21 | 19 | 50 |
| Clear water control | 93.3 | 50 | 34 | — |
| Total eclipse is dressed seed only | 53.3 | 7 | 7 | 79 |
It can be illustrated by table 4, after two methods of seed soaking and the processing of pouring root, biocontrol microorganisms of the present invention have take-all
Preventive effect is worked as with total eclipse net phase after preferable control efficiency, especially fermentation liquid dilute 5 times, and root irrigation mode control efficiency is higher than
Seed soaking.After all chemicals treatments, there is certain influence to emergence, shows as emergence evening, but at biocontrol microorganisms YB-F7 fermentation liquid
Emergence rate after reason is low to be above other medicaments.
In conclusion Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7 of the present invention is to small
Wheat Pathogen of Take-all, sheath blight fungus and root-rot sickle-like bacteria all have significant fungistatic effect；Therefore, Methylotrophic gemma bar of the present invention
Bacterium (Bacillus methylotrophicus) YB-F7 can be completely applied to plant soil caused by preparation prevention and treatment Pathogen of Take-all
It passes in the evil microbial inoculum of plant base rot disease caused by plant roots stem disease caused by disease, sheath blight fungus or root-rot sickle-like bacteria.
Claims (3)
1. a kind of Methylotrophic bacillus (Bacillus methylotrophicus) YB-F7, it is characterised in that: the bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.10834.
2. a kind of Methylotrophic bacillus described in claim 1 (Bacillus methylotrophicus) YB-F7
The plant soil-borne diseases caused by biological control Pathogen of Take-all, plant roots stem disease caused by sheath blight fungus or root-rot sickle-like bacteria
Application in caused plant base rot disease evil.
3. a kind of Methylotrophic bacillus described in claim 1 (Bacillus methylotrophicus) YB-F7
Plant roots stem disease or root-rot sickle-like bacteria caused by plant soil-borne diseases, sheath blight fungus caused by preventing and treating Pathogen of Take-all in preparation
Application in caused plant base rot disease evil microbial inoculum.
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