CN105053064A - Nuisanceless biological compound pesticide and preparation method thereof - Google Patents
Nuisanceless biological compound pesticide and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of biopesticides, and mainly discloses a nuisanceless biological compound pesticide and a preparation method thereof. The biological compound pesticide is manufactured from the raw materials of Ailanthus altissima Swingle, Cyrtomium fortunei, geranium wilfordii, fructus forsythiae, Scutellaria baicalensis, gallnut, areca-nut, coconut shell, radix sophorae flavescentis, bacillus subtilis, bacillus licheniformis, Streptomyces rimosus and streptomyces aureus. The Chinese herbal medicine extracts of Ailanthus altissima Swingle, Cyrtomium fortunei and the like are added to the pesticide, so that the antibacterial effect of the biopesticide is greatly improved; and moreover, the Chinese herbal medicine extracts are free of toxic action for people and livestock, so that there is no need to worry about causing harmful effects on people and livestock when the pesticide is used. The biopesticide disclosed by the invention has the characteristics of being high in efficiency, environment-friendly, free of secondary toxicity, residues, pollution or public nuisance and unlikely to generate resistance to drugs and keeping ecological balance, is important guarantee for realizing green ecological agriculture and green enterprises, and has wide prospects.
Description
Technical field
The invention belongs to field of biological pesticide, relate generally to a kind of harmless boilogical composite pesticide and preparation method thereof.
Background technology
The control of common flower diseases and pests mainly contains cultural control method, chemical control method and measure of biotic control, at present to adopt comparatively traditional cultural control to add chemical control pattern, general employing applies chemical pesticide, wipes out the means such as damage by disease and insect branches and leaves.But using chemical pesticide in a large number easily causes serious consequence for a long time, such as develops immunity to drugs, destroys the ecological balance, causes environmental pollution etc.Especially concerning ornamental flower, visitor is close with it, and its sight coexists with agricultural chemicals harmfulness, threatens visitor healthy.Meanwhile, the drug resistance population of damage by disease and insect increases, and also makes the balance of natural ecosystems be destroyed.Therefore along with the development of agricultural production, people yearn for green consumption, urgently wish a kind of biopesticide of nuisanceless, broad spectrum activity, wide region integrated control damage by disease and insect, to adapt to the demand in market and people.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of harmless boilogical composite pesticide and preparation method thereof, this agricultural chemicals fungistatic effect is remarkable, does not poison people and animals, and low production cost.
The technical solution adopted in the present invention is: a kind of harmless boilogical composite pesticide, and described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 5-8 part, rhizome of cyrtomium 2-4 part, geranium wilfordii 1-3 part, capsule of weeping forsythia 8-12 part, root of large-flowered skullcap 7-13 part, gall nut 10-15 part, betel nut 5-7 part, cocoa husk 15-23 part, kuh-seng 4-9 part, bacillus subtilis 0.2-0.5 part, bacillus licheniformis 0.3-0.5 part, be full of cracks Streptothrix 0.1-0.3 part and streptomyces aureus 0.2-0.4 part.
Described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described harmless boilogical composite pesticide, comprises the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix and streptomyces aureus are put into medium cultivate, add bacillus subtilis and bacillus licheniformis after 6-7 days, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 10-30 hour, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 6.5-7, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 1-2, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
Bacillus subtilis in the present invention (
bacillussubtilis) HS5B5, be preserved in China General Microbiological culture presevation administrative center, preserving number is: CGMCCNo.6088.Bacillus licheniformis (
bacilluslicheniformis) being preserved in China General Microbiological culture presevation administrative center, preserving number is CGMCC1.521.Be full of cracks Streptothrix (
streptomycesrimosus) being preserved in China General Microbiological culture presevation administrative center, preserving number is CGMCC4.748.Streptomyces aureus (
streptomycesaureus) being preserved in China General Microbiological culture presevation administrative center, preserving number is CGMCC4.6322.Above bacterial classification is except bacillus subtilis HS5B5 laboratory is preserved, and other is purchased from Shandong abyssopelagic organism Science and Technology Co., Ltd..
Compared with prior art, the present invention at least has following advantage and beneficial effect:
1, the present invention by adding the Chinese herbal medicine extract such as fruits of Ailanthus altissima, rhizome of cyrtomium in agricultural chemicals, not only substantially increase the fungistatic effect of biopesticide, and Chinese herbal medicine extract does not all have toxic action to people and animals, therefore the present invention need not worry to cause harmful effect to people and animals in use.Biological compound pesticide of the present invention is applicable to the extermination of disease and insect pest of common flowers, as Rose Powdery Mildew, Chinese Rose, Chinese herbaceous peony rust, Chinese rose scale, tree peony brown spot, tree peony gray mold, tree peony root nodule nematodosis etc., have high efficiency, the feature of environmental protection, without secondary toxicity, not easily develop immunity to drugs, noresidue, pollution-free, keep ecological balance, non-harmful feature, be the important guarantee realizing green ecological agricultural, green enterprises, there are bright prospects.
2, the present invention adds two kinds of bacillus again after first be full of cracks Streptothrix and streptomyces aureus being cultivated 6-7 days, can not only improve micro organism quantity, and can increase the antibacterial substance in biopesticide.The present invention in biopesticide, add be full of cracks Streptothrix and streptomyces aureus effectively can strengthen the effect of the damage by disease and insect such as pesticide control spot disease of the present invention, powdery mildew, nematodosis.
3, the fruits of Ailanthus altissima in the present invention obtains active component 2,6-dimethoxy-benzoquinone through process such as immersion, distillations, has the effect of significant anti-bacteria and anti-virus.Capsule of weeping forsythia glycoside material in the capsule of weeping forsythia and the matrine in kuh-seng cooperatively interact, and have bactericidal activity, can broad-spectrum sterilization and desinsection, have and tag and stomach poison function, have obvious control efficiency to multiple flower diseases and pests.Flavonoids scutelloside in the root of large-flowered skullcap all has very strong bactericidal activity to Gram-positive bacteria and negative bacteria, and does not produce drug resistance.Tannin in rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk can make its inactivation in conjunction with the surface protein of bacterium, then kills bacterium, and five kinds of Chinese herbal medicines combine, and content of tannin is higher, and effect is more remarkable.
Embodiment
A kind of harmless boilogical composite pesticide, described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 5-8 part, rhizome of cyrtomium 2-4 part, geranium wilfordii 1-3 part, capsule of weeping forsythia 8-12 part, root of large-flowered skullcap 7-13 part, gall nut 10-15 part, betel nut 5-7 part, cocoa husk 15-23 part, kuh-seng 4-9 part, bacillus subtilis 0.2-0.5 part, bacillus licheniformis 0.3-0.5 part, be full of cracks Streptothrix 0.1-0.3 part and streptomyces aureus 0.2-0.4 part; Described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described harmless boilogical composite pesticide, comprises the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix powder and streptomyces aureus powder are put into medium cultivate, add bacillus subtilis powder and bacillus licheniformis powder after 6-7 days, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 10-30 hour, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 6.5-7, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 1-2, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
In order to make the present invention have better implementation result, the medium described in step 2 is made up of potato, wheat bran, brown sugar and water, and wherein the parts by weight of each component are respectively: potato 19-21 part, wheat bran 15-20 part, brown sugar 2-3 part and 100 parts, water.
for making content of the present invention become apparent, below in conjunction with specific embodiment, describe the present invention.
embodiment 1:
A kind of harmless boilogical composite pesticide, described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 5 parts, rhizome of cyrtomium 2 parts, geranium wilfordii 1 part, the capsule of weeping forsythia 8 parts, the root of large-flowered skullcap 7 parts, gall nut 10 parts, betel nut 5 parts, cocoa husk 15 parts, kuh-seng 4 parts, bacillus subtilis 0.2 part, bacillus licheniformis 0.3 part, be full of cracks Streptothrix 0.1 part and streptomyces aureus 0.2 part; Described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described harmless boilogical composite pesticide, comprises the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix powder and streptomyces aureus powder are put into medium cultivate, add bacillus subtilis powder and bacillus licheniformis powder after 6d, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 10 hours, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 6.5, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 1, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
embodiment 2:
A kind of harmless boilogical composite pesticide, described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 8 parts, rhizome of cyrtomium 4 parts, geranium wilfordii 3 parts, the capsule of weeping forsythia 12 parts, the root of large-flowered skullcap 13 parts, gall nut 15 parts, betel nut 7 parts, cocoa husk 23 parts, kuh-seng 9 parts, bacillus subtilis 0.5 part, bacillus licheniformis 0.5 part, be full of cracks Streptothrix 0.3 part and streptomyces aureus 0.4 part; Described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described harmless boilogical composite pesticide, comprises the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix powder and streptomyces aureus powder are put into medium cultivate, add bacillus subtilis powder and bacillus licheniformis powder after 7 days, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 30 hours, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 7, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 2, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
embodiment 3:
A kind of harmless boilogical composite pesticide, described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 7 parts, rhizome of cyrtomium 3 parts, geranium wilfordii 2 parts, the capsule of weeping forsythia 10 parts, the root of large-flowered skullcap 10 parts, gall nut 13 parts, betel nut 6 parts, cocoa husk 19 parts, kuh-seng 7 parts, bacillus subtilis 0.3 part, bacillus licheniformis 0.4 part, be full of cracks Streptothrix 0.2 part and streptomyces aureus 0.3 part; Described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described harmless boilogical composite pesticide, comprises the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix powder and streptomyces aureus powder are put into medium cultivate, add bacillus subtilis powder and bacillus licheniformis powder after 7 days, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 20 hours, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 6.5, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 1.5, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
experimental study data
test one
Within on 05 13rd, 2015, in Luoyang, this ground, with choosing the sample of one piece of plantation tree peony as subjects, is divided into A, B, C tri-groups by international peony garden, and isolation growth.
A group uses the present invention, and every mu of ground sprays 40mL, converts clear water 30kg.B group uses in the present invention and removes component after Chinese herbal medicine extract, and every mu of ground sprays 40mL, converts clear water 30kg.C group does not use the present invention, and sprays clear water 30kg.
Result of the test: after one month, A group peony plant is tall and big vigorous, occurs without damage by disease and insect; B group peony plant is tall and big not as A group, has part damage by disease and insect to occur; C group tree peony growing way is more weak, has obvious damage by disease and insect phenomenon to occur.
testtwo
Within on 05 26th, 2015, choose the sample of one piece of plantation Chinese rose in Luolong District, Luoyang City as subjects, this ground is divided into A, B, C tri-groups, and isolation growth.
A group uses the present invention, and every mu of ground sprays 40mL, converts clear water 30kg.B group uses in the present invention and removes component after Chinese herbal medicine extract, and every mu of ground sprays 40mL, converts clear water 30kg.C group does not use the present invention, and sprays clear water 30kg.
Result of the test: after one month, A group Chinese rose plant growing way is vigorous, occurs without damage by disease and insect; B group Chinese rose plant is tall and big not as A group, has part damage by disease and insect to occur; C group Chinese rose growing way is more weak, has obvious damage by disease and insect phenomenon to occur.
Claims (3)
1. a harmless boilogical composite pesticide, is characterized in that: described biological compound pesticide is made up of the raw material of following parts by weight: fruits of Ailanthus altissima 5-8 part, rhizome of cyrtomium 2-4 part, geranium wilfordii 1-3 part, capsule of weeping forsythia 8-12 part, root of large-flowered skullcap 7-13 part, gall nut 10-15 part, betel nut 5-7 part, cocoa husk 15-23 part, kuh-seng 4-9 part, bacillus subtilis 0.2-0.5 part, bacillus licheniformis 0.3-0.5 part, be full of cracks Streptothrix 0.1-0.3 part and streptomyces aureus 0.2-0.4 part.
2. a kind of harmless boilogical composite pesticide as claimed in claim 1, is characterized in that: described bacillus subtilis is bacillus subtilis HS5B5.
3. the preparation method of a kind of harmless boilogical composite pesticide as claimed in claim 1 or 2, is characterized in that: comprise the following steps:
Step one, get each raw material by the parts by weight of above-mentioned each component, for subsequent use;
Step 2, be full of cracks Streptothrix and streptomyces aureus are put into medium cultivate, add bacillus subtilis and bacillus licheniformis after 6-7 days, cultivate and obtain mix bacterium agent after 3 days, for subsequent use;
Step 3, first fruits of Ailanthus altissima is crushed to 100 orders, then soaks 10-30 hour, soaked to be placed in Rotary Evaporators and heated, collect distillate, obtain extract I, for subsequent use; Be crushed to 100 orders by after the capsule of weeping forsythia and kuh-seng mixing, then join in boiling water, boil after 40 minutes and filter, filtrate reduced in volume, obtains extract II, for subsequent use; The root of large-flowered skullcap is crushed to 100 orders, joins in boiling water and boils 30 minutes, has boiled rear filtration, has obtained filtrate, then in filtrate, adds the sodium hydroxide that mass percent is 40%, regulates pH to 6.5-7, obtains mixed liquor, for subsequent use; In mixed liquor, add the ethanol that isopyknic percent by volume is 95%, suction filtration after stirring, then the filtrate obtained is heated to 50 DEG C, and adjust pH to 1-2, precipitation to be precipitated with the hydrochloric acid that concentration is 2mol/L, filter, collect solid, obtain extract II I, for subsequent use; By rhizome of cyrtomium, geranium wilfordii, gall nut, betel nut and cocoa husk mixing, be crushed to 300 orders, obtain mixed-powder, for subsequent use;
Step 4, the extract I first step 3 obtained, extract II, extract II I and mixed-powder mix, and then add the mix bacterium agent that step 2 obtains, stir, namely complete the preparation of harmless boilogical composite pesticide.
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| CN105994427A (en) * | 2016-05-27 | 2016-10-12 | 余海春 | Composition for preventing powdery mildew of Zanthoxylum armatum var.novemfolius |
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| CN111587895A (en) * | 2019-02-19 | 2020-08-28 | 安徽省农业科学院蚕桑研究所 | Biological bactericide for preventing and treating mulberry ring spot and preparation method thereof |
| CN110419530A (en) * | 2019-08-27 | 2019-11-08 | 彭玉柏 | A kind of pesticide made of Chinese medicinal herbs and its production technology |
| CN111345323A (en) * | 2020-04-17 | 2020-06-30 | 滨州学院 | Biological bactericide containing bacillus licheniformis |
| CN115399343A (en) * | 2022-10-31 | 2022-11-29 | 山东睿鹰制药集团有限公司 | Plant composite antibacterial agent containing peony extract and preparation method and application thereof |
| CN115399343B (en) * | 2022-10-31 | 2023-01-24 | 山东睿鹰制药集团有限公司 | Plant composite antibacterial agent containing peony extract and preparation method and application thereof |
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