CN106342939A - Biological agent for preventing and treating peanut root rot and preparation method thereof - Google Patents
Biological agent for preventing and treating peanut root rot and preparation method thereof Download PDFInfo
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- CN106342939A CN106342939A CN201610770347.3A CN201610770347A CN106342939A CN 106342939 A CN106342939 A CN 106342939A CN 201610770347 A CN201610770347 A CN 201610770347A CN 106342939 A CN106342939 A CN 106342939A
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- root rot
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a biological agent for preventing and treating peanut root rot. The biological agent for preventing and treating the peanut root rot comprises the following raw materials: 25 to 30 part of honeysuckle, 15 to 20 parts of cinnamon, 10 to 15 parts of cortex pseudolaricis, 5 to 10 parts of garlic, 10 to 15 parts of Chinese gall, 5 to 10 parts of Melia azedarach, 5 to 10 parts of aloe, 5 to 10 parts of tripterygium wilfordii, 8 to 10 parts of acorus calamus and 15 to 20 parts of a microbial agent. The invention also discloses a preparation method of the biological agent. The biological agent provided by the invention is a traditional Chinese medicine preparation, has easily available raw materials and low cost, has significant prevention and treatment effects on the peanut root rot, and has the advantages of no toxic or side effect, no residue and no environmental pollution.
Description
Technical field
The present invention relates to a kind of biological agent and preparation method thereof, especially relate to a kind of biology for Semen arachidis hypogaeae root rot
Medicament and preparation method thereof, belongs to biological agent technical field.
Background technology
The root rot of Semen arachidis hypogaeae all can occur in the period of duration of Semen arachidis hypogaeae.Disease plant is short and small, and blade turns yellow from bottom to top successively,
Dry up and come off, main root crust blackening is rotted, until whole strain is dead.This disease is propagated mainly by rainwater and field, seedling stage field ponding,
Ground temperature is low or sowing is too early, too deep, all preferably falls ill.
At present, main prevention and controls are: one, basis preventing and treating: efficent rotation, strictly choose seeds, bask seeds, site preparation is improved the soil, enrich corruption
Ripe fertilizer, draining of preventing waterlogging, enhance field management;2nd, chemical control: press grain weight with 50% carbendazol wettable powder
0.3% seed dressing, their early stage is prevented and treated with 50% 1000 times of liquid sprayings of carbendazim.
Above Therapeutic Method has certain curative effect, but effect is still unable to reach actual requirement, can reduce the economy of Semen arachidis hypogaeae
It is worth, and the medicament environmental pollution of time and effort consuming, use is serious, there is potential safety hazard.
Content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of have no toxic side effect, good being used for of therapeutic effect
Biological agent of preventing and treating Semen arachidis hypogaeae root rot and preparation method thereof.
The technical solution adopted in the present invention is:
A kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot, its raw material includes the parts by weight difference of following components and each component
For:
Flos Lonicerae: 25-30 part;
Cortex Cinnamomi: 15-20 part;
Cortex Pseudolaricis: 10-15 part;
Bulbus Allii: 5-10 part;
Galla Chinensiss: 10-15 part;
Melia azedarach L.: 5-10 part;
Aloe: 5-10 part;
Radix Tripterygii Wilfordii: 5-10 part
Rhizoma Acori Graminei: 8-10 part;
Microbial bacterial agent: 15-20 part.
Further preferably, its raw material can include following components and the parts by weight of each component are respectively as follows:
Flos Lonicerae: 25 parts;
Cortex Cinnamomi: 20 parts;
Cortex Pseudolaricis: 10 parts;
Bulbus Allii: 10 parts;
Galla Chinensiss: 15 parts;
Melia azedarach L.: 8 parts;
Aloe: 8 parts;
Radix Tripterygii Wilfordii: 8 parts
Rhizoma Acori Graminei: 8 parts;
Microbial bacterial agent: 15 parts.
And above-mentioned microbial bacterial agent includes streptomyces griseuses, bacillus subtilises and flora carrier, and described Lycoperdon polymorphum Vitt
Streptomycete, the ratio of weight and number of bacillus subtilises are: (3-5): 1.
And described flora carrier is wheat bran.
Additionally, the mass percent of the active component in above-mentioned microbial bacterial agent is 10-15%.
The invention also discloses a kind of preparation method of the biological agent for preventing and treating Semen arachidis hypogaeae root rot, walk including following
Rapid: according to the ratio of weight and number of each component, first by Flos Lonicerae, Cortex Cinnamomi, Cortex Pseudolaricis, Bulbus Allii, Galla Chinensiss, Melia azedarach L., Aloe, Thunder God
After rattan, Rhizoma Acori Graminei mix homogeneously, it is 1-3mm to particle diameter after pulverizing, is subsequently adding the water of 2-3 times of quality of mixture quality, decoct 2-3
Secondary, decoct 2-3h every time, filter after merging decoction liquor, concentrating the filtrate to relative density when 25 DEG C is: 1.10-1.20, obtains
Chinese medicine extract concentrated solution, after cooling, is added thereto to microbial bacterial agent, obtains biological agent after stirring, and in 2-5 DEG C
Temperature conditionss under stored refrigerated.
The invention has the beneficial effects as follows: biological agent of the present invention is a kind of Chinese medicine preparation, and raw material is easy to get, cost
Low, and there is significant prevention and treatment effect to the root rot of Semen arachidis hypogaeae, with more having no toxic side effect, noresidue, do not pollute ring
The advantage in border.Additionally, preparation method step of the present invention, simply it is easy to operation, is especially suitable for popularization and application.
Specific embodiment
With reference to specific embodiment, the present invention will be further described:
Flos Lonicerae: to streptococcus pneumoniae, meningococcus, bacillus pyocyaneus, tubercule bacillus, dysentery bacterium, deformation hammer
Bacterium etc. has antibacterial and bactericidal action, and infected by influenza, Orphan virus, herpesviruss, leptospira all have inhibitory action;
Cortex Cinnamomi: have bacteriostasis to gram positive bacteria and funguses;
Cortex Pseudolaricis: trichophyton schoenleini, acrothesium floccosum, rust little spore tinea bacterium, microsporon gypseum and white are read
Pearl bacterium has bactericidal action;
Bulbus Allii: there is antioxidant activity, bacteriostasis are strong;
Galla Chinensiss: to bacillus pyocyaneus, dysentery bacterium, Bacillus proteuss, escherichia coli, Bacillus typhi, bacillus enteritidiss, anthrax bacillus,
Diphtheria corynebacterium, staphylococcus aureuses, group B streptococcus and streptococcus pneumoniae all have inhibitory action;
Melia azedarach L.: insecticidal action is obvious;
Aloe: there is parasite killing, disinfection, and the nutrient such as rich in mineral substances, aminoacid and vitamin;
Radix Tripterygii Wilfordii: there is sterilization;
Rhizoma Acori Graminei: belong to environment friendly agricultural, can effectively prevent and treat the insect pests such as planthopper, rice leafhopper, rice green caterpillar, aphid, red spider;
The mutual compatibility of above each group split-phase, synergistic effect is good, can not only be that Semen arachidis hypogaeae provides nutrient, and have good killing
Bacterium effect, the prevention effect to root rot is higher than 85%, and can effectively suppress disease to develop, and enhances the disease-resistant performance of Semen arachidis hypogaeae, and
Above prescription is safe, environmentally friendly.
In the examples below, microbial bacterial agent includes streptomyces griseuses, bacillus subtilises and flora carrier, and described ash
Color streptomycete, the ratio of weight and number of bacillus subtilises are: 4:1, and flora carrier is the activity one-tenth in wheat bran, and microbial bacterial agent
The mass percent divided is 12.5%.
Embodiment 1:
By Flos Lonicerae: 25 parts;Cortex Cinnamomi: 15 parts;Cortex Pseudolaricis: 10 parts;Bulbus Allii: 5 parts;Galla Chinensiss: 10 parts;Melia azedarach L.: 5 parts;Aloe: 5
Part;Radix Tripterygii Wilfordii: 5 parts;Rhizoma Acori Graminei: after 8 parts of mix homogeneously, be 1-3mm to particle diameter after pulverizing, be subsequently adding 2 times of matter of mixture quality
The water of amount, decocts 3 times, decocts 2h every time, filters, concentrating the filtrate to relative density when 25 DEG C is after merging decoction liquor: 1.12,
Obtain Chinese medicine extract concentrated solution, after cooling, be added thereto to 15 parts of microbial bacterial agent, after stirring, obtain biological agent,
And under 2-5 DEG C of temperature conditionss stored refrigerated.
Embodiment 2:
By Flos Lonicerae: 30 parts;Cortex Cinnamomi: 20 parts;Cortex Pseudolaricis: 15 parts;Bulbus Allii: 10 parts;Galla Chinensiss: 15 parts;Melia azedarach L.: 10 parts;
Aloe: 10 parts;Radix Tripterygii Wilfordii: 10 parts;Rhizoma Acori Graminei: after 10 parts of mix homogeneously, be 1-3mm to particle diameter after pulverizing, be subsequently adding mixture
The water of 3 times of quality of quality, decocts 2 times, decocts 3h every time, filters, concentrate the filtrate to relatively close when 25 DEG C after merging decoction liquor
Spend and be: 1.18, obtain Chinese medicine extract concentrated solution, after cooling, be added thereto to 20 parts of microbial bacterial agent, obtain after stirring
Biological agent, and stored refrigerated under 2-5 DEG C of temperature conditionss.
Embodiment 3:
By Flos Lonicerae: 28 parts;Cortex Cinnamomi: 18 parts;Cortex Pseudolaricis: 12 parts;Bulbus Allii: 8 parts;Galla Chinensiss: 12 parts;Melia azedarach L.: 8 parts;Aloe: 8
Part;Radix Tripterygii Wilfordii: 8 parts;Rhizoma Acori Graminei: after 9 parts of mix homogeneously, be 1-3mm to particle diameter after pulverizing, be subsequently adding 3 times of matter of mixture quality
The water of amount, decocts 3 times, decocts 3h every time, filters, concentrating the filtrate to relative density when 25 DEG C is after merging decoction liquor: 1.16,
Obtain Chinese medicine extract concentrated solution, after cooling, be added thereto to 18 parts of microbial bacterial agent, after stirring, obtain biological agent,
And under 2-5 DEG C of temperature conditionss stored refrigerated.
Embodiment 4:
By Flos Lonicerae: 25 parts;Cortex Cinnamomi: 20 parts;Cortex Pseudolaricis: 10 parts;Bulbus Allii: 10 parts;Galla Chinensiss: 15 parts;Melia azedarach L.: 8 parts;Aloe:
8 parts;Radix Tripterygii Wilfordii: 8 portions of Rhizoma Acori Gramineis: after 8 parts of mix homogeneously, be 1-3mm to particle diameter after pulverizing, be subsequently adding 2 times of matter of mixture quality
The water of amount, decocts 3 times, decocts 2h every time, filters, concentrating the filtrate to relative density when 25 DEG C is after merging decoction liquor: 1.18,
Obtain Chinese medicine extract concentrated solution, after cooling, be added thereto to 18 parts of microbial bacterial agent, after stirring, obtain biological agent,
And under 2-5 DEG C of temperature conditionss stored refrigerated.
Test experiments are compareed for the biological agent that the above embodiments 1-5 prepare as follows:
Compareing one group is clear water matched group, and two groups of comparison is 50% 1000 times of liquid of carbendazim, and experimental group is four groups, applies respectively
500 times of liquid of the biological agent that embodiment 1-4 prepares.Every group all selects each 100 of the Semen arachidis hypogaeae with root rot, all groups
Application process be: blade face conventional spray, spray time is consistent, and its result is as shown in table 1:
Table 1: the root rot therapeutic effect comparison table of Semen arachidis hypogaeae
As shown in Table 1: field efficacy >=85% of biological agent of the present invention, and apparently higher than 50% 1000 times of carbendazim
Liquid.
The present invention is not limited to embodiment as above, in the category without departing from basic fundamental thought of the present invention
Interior, the operator of relevant industries can carry out various deformation to it, and, the above-mentioned deformation carrying out should belong to the right of the present invention will
Seek scope.
Claims (6)
1. a kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot is it is characterised in that its raw material includes following components and each component
Parts by weight be respectively as follows:
Flos Lonicerae: 25-30 part;
Cortex Cinnamomi: 15-20 part;
Cortex Pseudolaricis: 10-15 part;
Bulbus Allii: 5-10 part;
Galla Chinensiss: 10-15 part;
Melia azedarach L.: 5-10 part;
Aloe: 5-10 part;
Radix Tripterygii Wilfordii: 5-10 part
Rhizoma Acori Graminei: 8-10 part;
Microbial bacterial agent: 15-20 part.
2. a kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot according to claim 1 is it is characterised in that its raw material bag
Include following components and the parts by weight of each component are respectively as follows:
Flos Lonicerae: 25 parts;
Cortex Cinnamomi: 20 parts;
Cortex Pseudolaricis: 10 parts;
Bulbus Allii: 10 parts;
Galla Chinensiss: 15 parts;
Melia azedarach L.: 8 parts;
Aloe: 8 parts;
Radix Tripterygii Wilfordii: 8 parts
Rhizoma Acori Graminei: 8 parts;
Microbial bacterial agent: 15 parts.
3. a kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot according to claim 1 and 2 is it is characterised in that described
Microbial bacterial agent include streptomyces griseuses, bacillus subtilises and flora carrier, and described streptomyces griseuses, hay spore
The ratio of weight and number of bacillus is: (3-5): 1.
4. a kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot according to claim 3 is it is characterised in that described bacterium
Group's carrier is wheat bran.
5. according to a kind of biological agent for preventing and treating Semen arachidis hypogaeae root rot described in claim 3 it is characterised in that described micro- life
The mass percent of the active component in thing microbial inoculum is 10-15%.
6. a kind of as claimed in claim 1 preparation method being used for preventing and treating the biological agent of Semen arachidis hypogaeae root rot it is characterised in that
Comprise the following steps: according to the ratio of weight and number of each component, first by Flos Lonicerae, Cortex Cinnamomi, Cortex Pseudolaricis, Bulbus Allii, Galla Chinensiss, hardship
After chinaberry, Aloe, Radix Tripterygii Wilfordii, Rhizoma Acori Graminei mix homogeneously, it is 1-3mm to particle diameter after pulverizing, is subsequently adding 2-3 times of quality of mixture quality
Water, decoct 2-3 time, every time decoct 2-3h, merge decoction liquor after filtration, concentrating the filtrate to relative density when 25 DEG C is:
1.10-1.20, obtains Chinese medicine extract concentrated solution, after cooling, is added thereto to microbial bacterial agent, obtains biology after stirring
Medicament, and stored refrigerated under 2-5 DEG C of temperature conditionss.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109287679A (en) * | 2018-11-23 | 2019-02-01 | 王变芝 | Biological agent and preparation method thereof for preventing and treating peanut root rot |
CN111602686A (en) * | 2020-06-15 | 2020-09-01 | 山东省农业科学院植物保护研究所 | Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109287679A (en) * | 2018-11-23 | 2019-02-01 | 王变芝 | Biological agent and preparation method thereof for preventing and treating peanut root rot |
CN111602686A (en) * | 2020-06-15 | 2020-09-01 | 山东省农业科学院植物保护研究所 | Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof |
CN111602686B (en) * | 2020-06-15 | 2021-06-15 | 山东省农业科学院植物保护研究所 | Pharmaceutical composition for preventing peanut root rot based on limonene and preparation method thereof |
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