CN106797965A - Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine - Google Patents
Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine Download PDFInfo
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- CN106797965A CN106797965A CN201611174790.0A CN201611174790A CN106797965A CN 106797965 A CN106797965 A CN 106797965A CN 201611174790 A CN201611174790 A CN 201611174790A CN 106797965 A CN106797965 A CN 106797965A
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- tube lycoris
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The invention discloses a kind of application of short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine, the short-tube lycoris flower extract preparation method gathers the short-tube lycoris petal withered or withered first, running water dries after cleaning up, then the petal samples that will be cleaned after drying using refiner are crushed, and petal and ethanol solution are pressed into mass volume ratio 1:10~30g/L mixes, and extracts 24~72h, and short-tube lycoris flower extract is can obtain after filtering petal impurity.The short-tube lycoris flower extract is in maca root rot, application in dry rot of potato and water lily black spot, with wide spectrum antagonism, extracting method is simple, with low cost, turns waste into wealth, the efficient antagonism maca root rot of energy, the multiple diseases such as dry rot of potato and water lily black spot, and fungistatic effect is significantly, suitable popularization and application.
Description
Technical field
The present invention relates to treat or the medicine of pre- anti-fungal in application, in particular to a kind of short-tube lycoris flower extract prepare
Treat or prevent the application in mycotic medicine.
Background technology
Maca (Lepidium meyenii) is the grass for originating in the Andean Cruciferae separate row Vegetable spp of South America
This plant, the nutrition of maca root and bioactive ingredients enrich, various with compressive resistance, antifatigue, antidepression, raising fertility etc.
Health-care efficacy, see (remaining Longjiang, inscription on ancient bronze objects is heard, Wu Yuanxi, waits the botany and its pharmacological research overview [J] days of macas
Right Study on product and exploitation, 2002 (5)：71-74.).In recent years, the country increases and maca medicinal ingredient is excavated, by extensive
Food and medicine field of health care products are applied to, there is wide domestic and international market demand.
However, maca root rot generally occurs in China's maca growing area, every year because disease causes huge economic damage
Lose, cause the great attention of local maca planting technology personnel and related researcher, see (remaining Longjiang, inscription on ancient bronze objects hears macas
Plantation processing and nutrition and health care [M] Beijing：Chemical Industry Press, 2015：68-69.).Preventing and treating to maca root rot at present
Generally controlled by chemical prevention, excessive use chemical bactericide has harm human body health, medicament residue destruction ecological
Environment, trigger pathogen the shortcomings such as to develop immunity to drugs, be unfavorable for maca industry sustainable development.Therefore, safety, height are sought
Effect and economic biogenic pesticide are particularly important.
The relevant report that plant extracts suppresses plant disease is had both at home and abroad, and such as Wang Xiaojuan have studied stemonine etc. 5
As a result Plant Extracts show that Extractives From Melia Azedarach L is the most obvious to water mo(u)ld inhibitory action to the inhibitory action of water mo(u)ld, please
See (inhibitory action [J] Anhui agronomy circular of 5 Plant Extracts such as Wang Xiaojuan, Hu Qing, Lin Gang stemonines to water mo(u)ld
(upper half monthly magazine), 2012 (23)：38-42.)；Zhou Jianhong etc. uses the water of 20 kinds of plants of growth rate method preliminary screening to extract
Thing shows oil tea Bacteria erwinia, the bioactivity of anthrax bacteria, the control effect in forest of different plant extracts：When cloves,
The mass ratio of Baical Skullcap root P.E and Tea Saponin is 1:1:When 1, the prevention effect to oil tea Major Diseases is best, and reachable 80%, together
When can be by the diseased fruit rate of oil tea reduction about 60%, see (Zhou Jianhong, Liu Junang, Deng little Jun wait plant extracts to oil tea
Bacteriostasis [J] the Sino-South African Forestry University of Science and Technology journal of Major Diseases, 2011 (04)：42-45.).Zheng Peiwei etc. reports profit
The insecticide combined with short-tube lycoris bulb extract and insecticides adjuvant to Tetranychus cinnabarinus, diamondback moth, the prevention effect such as vegetable aphid worm,
See (Zheng Peiwei, Chen Anliang, Kuang Jungen, short-tube lycoris bulb extract insecticide and method for preparing extractive,
201210054345.6)；Ge Xizhen etc. disclose it is a kind of prevent and treat wettable powder of dry rot of potato and preparation method thereof, should
The active ingredient of wettable powder for rascal, the dried immature fruit of citron orange, Herba Andrographitis, the Chinese bulbul and vernonia anthelmintica medicinal material ethanol extract with it is small
The mixture of bark of a cork tree alkali, the prevention effect to dry rot of potato reaches more than 80%, see (Ge Xizhen, Pei Qinghui, Li Keyi,
Wettable powder of dry rot of potato and preparation method thereof, 20151079627.7 are prevented and treated Deng is a kind of).
Yet there are no by the use of this position of the flower of short-tube lycoris plant as raw material effective component extracting prevent and treat maca root rot,
The relevant report of dry rot of potato and water lily black spot.The existing artificial growth of short-tube lycoris is more and more, and after the florescence, petal is withered
Wither, do not utilized by people.
The content of the invention
Preparing treatment or preventing mycotic medicine the present invention seeks to there are provided a kind of short-tube lycoris flower extract
Application.The short-tube lycoris flower extract comes from what is withered as the easy biological source bactericide of efficient, safety, preparation, its raw material
Short-tube lycoris petal, production cost is extremely low, turns waste into wealth.Short-tube lycoris flower extract is preparing treatment or prevention maca root rot, potato
Application in dry rot and water lily blackspot medicine, extract bacteriostasis rate experiment shows that the short-tube lycoris flower extract is to maca root-rot
The bacteriostasis rate of disease reaches more than 80%, and the bacteriostasis rate to dry rot of potato and water lily blackspot pathogen reaches more than 70%.
To achieve the above object, a kind of short-tube lycoris flower extract that the present invention is provided is preparing treatment or is preventing mycotic medicine
Application in thing, the nosomycosis is maca root rot, dry rot of potato or water lily black spot.
Further, the preparation method of the short-tube lycoris flower extract, comprises the following steps：
1) the short-tube lycoris petal withered or withered is gathered, is dried after cleaning up,
2) petal samples that will be cleaned after drying are homogenized and crush,
3) petal and ethanol solution are pressed into mass volume ratio 1:10~30g/L mixes, and extracts 24~72h, and filtering petal is miscellaneous
Short-tube lycoris flower extract is can obtain after matter, wherein, ethanol solution mass fraction is 60~90%.
Yet further, the step 3) in, the mass fraction of ethanol solution is 70~85%.
Yet further, the petal and ethanol mass volume ratio 1:15~25g/L.
Application of the above-mentioned short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine, it applies basic process
It is：
A. using filter paper flat board opposite culture method research short-tube lycoris flower extract to the antagonism of oil tea Major Diseases.It is accurate
Get the filter paper and sterilization treatment of a diameter of 7mm ready, and 2min is infiltrated in short-tube lycoris flower extract；The a diameter of 7mm that will be accomplished fluently
Cause of disease bacteria cake and infiltration after filter paper be placed on plate center line, cause of disease bacteria cake be placed in flat board center, be impregnated with short-tube lycoris
The filter paper of flower extract is placed in 28 DEG C of constant incubator cultures respectively apart from plate center 1.5cm, and every 24h ultra-clean
5 μ L short-tube lycoris flower extracts of aseptic absorption infiltrate 1 filter paper in workbench, observe antibacterial with it's too late size after 5-7 days, each
Treatment is repeated 3 times.
Bacteriostasis rate=(control colony diameter-treatment colony diameter)/(control colony diameter -7) × 100%.
B. antagonism of the short-tube lycoris flower extract to oil tea Major Diseases is studied using cultivation.By short-tube lycoris flower extract with
About 50 DEG C of PDA solid mediums are according to 1:Culture dish is poured into after 19 mixings, is put into a diameter of 7mm's in flat board center after cooling
Oil tea the main pathogenic fungi bacteria cake, is control with sterilized water, and each treatment is repeated 3 times.Pathogen colony diameter is measured after 5 days.
Bacteriostasis rate=(control colony diameter-treatment colony diameter)/(control colony diameter -7) × 100%.
The beneficial effects of the present invention are：
1) be found that short-tube lycoris flower extract first to maca root rot, dry rot of potato and water lily black spot be respectively provided with compared with
Strong suppression and virulence.Antimicrobial spectrum extensively, can effectively reduce cost accounting.
2) cultivation experiment bacteriostasis rate shows：The short-tube lycoris flower extract bacteriostasis rate of maca root rot is reached 80% with
On, the bacteriostasis rate to dry rot of potato and water lily blackspot pathogen reaches more than 70%.
Brief description of the drawings
Fig. 1 is antagonistic effect schematic diagram of the short-tube lycoris flower extract to maca root-rot pathogen；
Fig. 2 is antagonistic effect schematic diagram of the short-tube lycoris flower extract to dry rot of potato opportunistic pathogen；
Fig. 3 is antagonistic effect schematic diagram of the short-tube lycoris flower extract to water lily blackspot pathogen；
Brief description of the drawings：A is respectively maca root-rot pathogen, dry rot of potato opportunistic pathogen and water lily blackspot in wherein Fig. 1-3
Pathogen with the addition of short-tube lycoris in the growth conditions being not added with the culture medium of short-tube lycoris flower extract, B for three of the above pathogen
Growth conditions on the culture medium of flower extract, as a result show that the short-tube lycoris flower extract has well to these three disease funguses
Antagonism is especially notable to maca root rot fungistatic effect.
Specific embodiment
In order to preferably explain the present invention, below in conjunction with the specific embodiment main contents that the present invention is furture elucidated, but
Present disclosure is not limited solely to following examples.
Bacterium source：Maca root rot Fusarium avenaceum are with document report bacterial strain (Logrieco A, Rizzo
A,Ferracane R,et al.Occurrence ofbeauvericin and enniatins in wheat affected
by Fusariumavenaceumhead blight[J].Applied and EnvironmentalMicrobiology,
2002,68(1):82-85.)；Dry rot of potato Fusarium equiseti with document report bacterial strain (Niu Xiuqun, Li Jinhua,
Zhang Junlian, waits change [J] Acta Pratacultures of sickle-like bacteria in Gansu Province irrigated arid area continuous cropping potato rhizosphere soil, 2011,20
(4):236-243.)；Water lily black spot Phoma herbarum, with document report bacterial strain, (Zhao Dandan, Wang Yun, Fu Xiaoping wait
Taiwan green jujube causes the separation identification of rotten microorganism and its suppresses research [J] southwest agricultural journal, 2016,29 (2):379-
384.)。
Embodiment 1
The preparation method of short-tube lycoris flower extract 1, its step is：
1) the short-tube lycoris petal withered or withered is gathered, running water dries after cleaning up,
2) petal samples that will be cleaned after drying using refiner are homogenized and crushed, according to mass volume ratio 1：10g/L will spend
Valve and mass fraction are 90% ethanol mixing, extract 24h, and it is short to obtain short-tube lycoris flower extract Isosorbide-5-Nitrae DEG C after filtering petal impurity
Phase preservation.
Embodiment 2
The preparation method of short-tube lycoris flower extract 2, its step is：
1) the short-tube lycoris petal withered or withered is gathered, running water dries after cleaning up,
2) petal samples that will be cleaned after drying using refiner are crushed, according to mass volume ratio 1：20g/L by petal and
Mass fraction is 60% ethanol mixing, extracts 72h, and obtain 2,4 DEG C of short-tube lycoris flower extract after filtering petal impurity protects in short term
Hide.
Embodiment 3
The preparation method of short-tube lycoris flower extract 3, its step is：
1) the short-tube lycoris petal withered or withered is gathered, running water dries after cleaning up,
2) petal samples that will be cleaned after drying using refiner are crushed, according to mass volume ratio 1：15g/L by petal and
Mass fraction is 80% ethanol mixing, extracts 48h, and obtain 3,4 DEG C of short-tube lycoris flower extract after filtering petal impurity protects in short term
Hide.
Embodiment 4
Above-mentioned short-tube lycoris flower extract is preparing treatment or prevention maca root rot, dry rot of potato and water lily black spot disease
Application in opportunistic pathogen medicine, its flat board opposite culture method step is：
The filter paper and sterilization treatment of a diameter of 7mm are got out, and 2min is infiltrated in short-tube lycoris flower extract；By what is accomplished fluently
Filter paper after the cause of disease bacteria cake of a diameter of 7mm and infiltration is placed on plate center line, and cause of disease bacteria cake is placed in flat board center, leaching
Profit has the filter paper of short-tube lycoris flower extract respectively apart from plate center 1.5cm, is placed in 28 DEG C of constant incubator cultures, and every
24h 5 μ L short-tube lycoris flower extracts of aseptic absorption in superclean bench infiltrate 1 filter paper, observe antibacterial with it's too late after 5-7 days
Size, each treatment is repeated 3 times.The results are shown in Table 1.Result shows short-tube lycoris flower extract to maca root rot, dry rot of potato
And water lily black spot has good antagonism, wherein it is optimal to the bacteriostasis rate of maca root rot, reach more than 80%.
Bacteriostasis rate of the short-tube lycoris flower extract of table 1 to several fungal diseases
Data are mean+SD (similarly hereinafter) in table,
Bacteriostasis rate=(control colony diameter-treatment colony diameter)/(control colony diameter -7) × 100%.
Embodiment 5
Above-mentioned short-tube lycoris flower extract is preparing treatment or prevention maca root rot, dry rot of potato and water lily black spot disease
Application in opportunistic pathogen medicine, its cultivation step is：
PDA solid mediums by short-tube lycoris flower extract with about 50 DEG C are according to 1:Culture dish is poured into after 19 mixings, after cooling
Maca root rot, dry rot of potato and the water lily black spot bacteria cake of a diameter of 7mm are respectively put into flat board center, with sterilized water
It is control, each treatment is repeated 3 times.Pathogen colony diameter is measured after 7 days.
Bacteriostasis rate=(control colony diameter-treatment colony diameter)/(control colony diameter -7) × 100%.
The display short-tube lycoris flower extract of table 2 reaches more than 80% to the bacteriostasis rate of maca root rot, to dry rot of potato and
The bacteriostasis rate of water lily blackspot pathogen reaches more than 70%, illustrates that the antimicrobial spectrum of the extract is wider.
Bacteriostasis rate of the short-tube lycoris flower extract of table 2 to several fungal pathogens
Other unspecified parts are prior art.Although above-described embodiment is made that to the present invention and retouch in detail
State, but it is only a part of embodiment of the invention, rather than whole embodiments, people can also according to the present embodiment without
Other embodiment is obtained under the premise of creativeness, these embodiments belong to the scope of the present invention.
Claims (4)
1. application of a kind of short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine, it is characterised in that：It is described true
Bacterium disease is maca root rot, dry rot of potato or water lily black spot.
2. application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine according to claim 1, it is special
Levy and be：The preparation method of the short-tube lycoris flower extract, comprises the following steps：
1) the short-tube lycoris petal withered or withered is gathered, is dried after cleaning up,
2) petal samples that will be cleaned after drying are homogenized and crush,
3) petal and ethanol solution are pressed into mass volume ratio 1:10~30g/L mixes, and 24~72h is extracted, after filtering petal impurity
Short-tube lycoris flower extract is can obtain, wherein, ethanol solution mass fraction is 60~90%.
3. the preparation method of short-tube lycoris flower extract according to claim 2, its feature is being：The step 3) in, ethanol
The mass fraction of solution is 70~85%.
4. according to Claims 2 or 3 short-tube lycoris flower extract preparation method, its feature is being：The petal and ethanol
Mass volume ratio 1:15~25g/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074145A (en) * | 2018-01-26 | 2019-08-02 | 南开大学 | Application of the Lycoris aurea ethanol extract on pesticide |
| CN110326626A (en) * | 2019-08-05 | 2019-10-15 | 云南中医药大学 | The new application of lepidium seed volatile oil |
| CN112493260A (en) * | 2021-01-12 | 2021-03-16 | 福建农林大学 | Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613257A (en) * | 2012-03-05 | 2012-08-01 | 杭州绿普达生物科技有限公司 | Lycoris radiata bulb extractive pesticide and method for preparing extractive |
-
2016
- 2016-12-19 CN CN201611174790.0A patent/CN106797965B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613257A (en) * | 2012-03-05 | 2012-08-01 | 杭州绿普达生物科技有限公司 | Lycoris radiata bulb extractive pesticide and method for preparing extractive |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074145A (en) * | 2018-01-26 | 2019-08-02 | 南开大学 | Application of the Lycoris aurea ethanol extract on pesticide |
| CN110074145B (en) * | 2018-01-26 | 2022-01-28 | 南开大学 | Application of michelia figo ethanol extract in pesticide |
| CN110326626A (en) * | 2019-08-05 | 2019-10-15 | 云南中医药大学 | The new application of lepidium seed volatile oil |
| CN110326626B (en) * | 2019-08-05 | 2021-05-14 | 云南中医药大学 | New application of semen lepidii volatile oil |
| CN112493260A (en) * | 2021-01-12 | 2021-03-16 | 福建农林大学 | Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi |
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