CN110326626A - The new application of lepidium seed volatile oil - Google Patents
The new application of lepidium seed volatile oil Download PDFInfo
- Publication number
- CN110326626A CN110326626A CN201910715506.3A CN201910715506A CN110326626A CN 110326626 A CN110326626 A CN 110326626A CN 201910715506 A CN201910715506 A CN 201910715506A CN 110326626 A CN110326626 A CN 110326626A
- Authority
- CN
- China
- Prior art keywords
- volatile oil
- lepidium seed
- pathogenic bacteria
- seed volatile
- chinese medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses a kind of lepidium seed volatile oil in new application agriculturally；That is its application in prevention and treatment Chinese medicine or crop root rot or the application in the pathogenic bacteria that prevention and treatment causes Chinese medicine or crop overground part that disease occurs；The experimental results showed that lepidium seed volatile oil is to causing, 4 kinds of main pathogenic bacteria Fusarium oxysporums of notoginseng root rot generation, Fusarium solani, rotten mould and breaking-up column spore is mould and the mycelia growth of 3 kinds of pathogenic bacteria botrytis cinereas of Radix Notoginseng overground part disease generation, colletotrichum gloeosporioides Penz, Rhizoctonia solani Kuhn is caused to have very high inhibition to act on, when concentration is 50mg/mL, its inhibiting rate has all reached 100%, and volatile oil can effectively inhibit the growth of the Fusarium oxysporum on Radix Notoginseng plant as the result is shown for experiment made on the living；And volatile oil is volatile, has many advantages, such as tourette production of aromatic, therefore with the chemical fertilizer of its exploitation or pesticide will have safely, effectively, low-residual.
Description
Technical field
The present invention relates to the purposes of lepidium seed volatile oil more particularly to lepidium seed volatile oil to Chinese medicine or crop root rot
And the preventive and therapeutic effect to Chinese medicine or crop overground part multiple diseases, belong to biological pesticide technical field.
Background technique
Chemical agent is the medicament for having inhibiting effect to microorganism, according to interaction property chemical agent can be divided fungicide and
Bacteriostatic agent, bacteriostatic agent does not destroy the plasm of microorganism, and only suppresses the synthesis of neoblast substance, prevents microorganism from increasing
It grows；Microbe species, the length of time of Chemical treatment microorganism, temperature height and microorganism local environment etc., all shadow
Ring chemical agent sterilization or antibacterial ability and effect；Being excessively used for chemical agent not only economically causes to waste, greatly
The input cost for increasing peasant greatly more seriously polluted soil and reduce medical material quanlity, there is " 3R " (residual, drug resistance
And regeneration) problem；
Plant volatile oil is that one kind for being present in plant has aromatic odor, can come out with steam distillation and and water
Not miscible volatility oily ingredient, volatile oil be a mixture, component is complex, in volatile oil component with terpene at
It is point common, in addition, still containing small molecule aliphatic compound and small molecule aromatic compound；Plant volatile oil is applied not only to pass
System medicine, food, tobacco, alcohol and tobacco, it may also be used for plant protection (desinsection, antibacterial, weeding etc.), plant volatile oil conduct
Botanical pesticide is applied to plant protection art, consistent with the public nuisance-free agricultural chemicals theory of suggestion.In this sense, plant is waved
Hair oil can also be classified as third generation pesticide.
Lepidium seed is crucifer, and the dry mature seed of Lepidium apetalum Lepidium apetalum Willd. is practised
Claim " lepidii,semen ", there is purging the lung of pathogenic fire sending down abnormally ascending, eliminating phlegm and relieving asthma, inducing diuresis for removing edema, the effect of expelling the heat-evil by heresy.
Currently, having no that document relevant to technical solution of the present invention discloses.
Summary of the invention
It is an object of that present invention to provide a kind of new applications of lepidium seed volatile oil, i.e., it is in prevention and treatment Chinese medicine or crop root-rot
Application in disease；The pathogenic bacteria are Fusarium oxysporum (Fusarium oxysporum), Fusarium solani (Fusarium
Solani), rotten mould (Pythium aphanidermatum), breaking-up column spore mould (Cylindrocarpon destructans) etc.
Lepidium seed volatile oil of the present invention, which also can be applicable to prevention and treatment, causes Chinese medicine or crop overground part that causing a disease for disease occurs
In bacterium；The pathogenic bacteria are botrytis cinerea (Botrytis cinerea), colletotrichum gloeosporioides Penz (Colletotrichum
Gloeosporioides), Rhizoctonia solani Kuhn (Rhizoctonia solani) etc..
The purposes of lepidium seed volatile oil of the present invention is that lepidium seed volatile oil is used as to preparation prevention and treatment Chinese medicine or work
Object root rot or prevention and treatment cause the preparation of the pathogenic bacteria of Chinese medicine or crop overground part generation disease, i.e., with lepidium seed volatile oil
For active constituent, the application in the bacteria preparation that causes a disease is prevented and treated in preparation.
The ingredient (or effective component) of the present invention for preventing and treating the bacteria preparation that causes a disease is lepidium seed volatile oil, can also be added one
Acceptable auxiliary material in kind or plurality of Chinese and crop disease control preparation, or play and cooperate with other active components compounding
Bacteriostasis.
Lepidium seed volatile oil can be prepared into the pesticide of various dosage forms or the chemical fertilizer of various forms by prior art device.
Lepidium seed volatile oil is extracted by the conventional steam way of distillation in the present invention, anhydrous sodium sulfate drying and volatilizing oil and
It is made, the preparation method is simple, and required reagent cost is low, is conducive to mass production.
Lepidium seed volatile oil is as a kind of natural Fungicidal active substance, its activity disappears or by microorganism point after use
Solution, no pollution to the environment, to safety of human and livestock；Since lepidium seed volatile oil is Extracts from Plant Recourses, effective component is complicated various,
Be the mechanism of action of multicomponent multiple target point to pathogen so that target organisms be not likely to produce several ingredients of certain or certain are generated it is anti-
Pharmacological property, to obtain lasting control efficiency；Compared with chemical synthetic pesticide, the effective component of lepidium seed volatile oil is volatile,
It is degradable, it is antibacterial without sterilizing, therefore be not easy to cause damages to other beneficial microbes, improve soil micro-ecosystem structure, promotes
Plant and microorganism coevolution, preserve the ecological environment, and improve the quality of Chinese medicine or crop.
Lepidium seed volatile oil can reduce plant disease incidence and the state of an illness by inhibiting pathogen spore germination and mycelia growth
Index.
The lepidium seed volatile oil solution of 50mg/mL is prepared, solvent is DMSO and volumetric concentration containing volumetric concentration 1%
The aqueous solution of 0.1% Tween 80,4 kinds of main pathogenic bacteria point spore reaping hooks that notoginseng root rot occurs for test lepidium seed volatile oil
Bacterium (Fusarium oxysporum), Fusarium solani (Fusarium solani), rotten mould (Pythium
Aphanidermatum) and the effect of column spore mould (Cylindrocarpon destructans) is damaged, and with leading to Radix Notoginseng
3 kinds of pathogenic bacteria botrytis cinereas (Botrytis cinerea), the colletotrichum gloeosporioides Penz (Colletotrichum of top disease generation
Gloeosporioides), the effect of Rhizoctonia solani Kuhn (Rhizoctonia solani) mycelia growth；Lepidium seed as the result is shown
Volatile oil has the bacteriostasis of wide spectrum to 7 kinds of pathogens and bacteriostasis rate is high, no matter to aerial part fungi or under ground portion
Fungi inhibiting rate has all reached 100%, has an inhibiting effect to spore germination and mycelia growth, experiment made on the living volatile oil as the result is shown
The growth of the Fusarium oxysporum on Radix Notoginseng plant can effectively be inhibited.
Low, preparation method is simple, easy to use, high-efficient, volatile, low with putting into for lepidium seed volatile oil of the present invention
The characteristics of poison, low-residual；In the case where country greatly develops the overall background of organic agriculture, lepidium seed hair oil is more satisfactory to overcome three
The chemical agent substitute product of seven diseases are of great significance to the sustainable development of agricultural, have in agricultural production broader
Application prospect.
Detailed description of the invention
Fig. 1 is that lepidium seed volatile oil is mould to Radix Notoginseng pathogen Fusarium oxysporum (1), Fusarium solani (2), breaking-up column spore
(3), the inhibiting effect result that rotten mould (4), botrytis cinerea (5), colletotrichum gloeosporioides Penz (6), Rhizoctonia solani Kuhn (7) grow；
Fig. 2 is the inhibiting rate result that lepidium seed volatile oil and hymexazol grow 377 kinds of pathogen bacterium colonies；
Fig. 3 is Radix Notoginseng seedling growth figure in lepidium seed volatile oil live test, and wherein a is lepidium seed volatilization oil processing group；b
For positive controls；C is negative control.
Specific embodiment
Below by drawings and examples, invention is further described in detail, but the scope of the present invention is not limited to
The content, method is conventional method unless otherwise specified in embodiment, and the reagent used is routine unless otherwise specified
Commercial reagent or the reagent prepared according to a conventional method.
Embodiment 1: the preparation of lepidium seed volatile oil
(1) water that 8 times of its quality is added in the lepidium seed skin of 100g chopping, impregnates 2h, by the way of heating distillation,
Using the volatile oil in steam extraction lepidium seed, extraction time 6h, condensation collects and slips out liquid；
(2) what is extracted slips out the drying of liquid anhydrous sodium sulfate, removes contained humidity to get lepidium seed volatile oil, puts
Into in brown sample bottle, sealing, -20 DEG C are saved for use.
Embodiment 2: the activation of pathogenic bacteria
(1) potato dextrose agar (PDA): potato 200g, glucose 20g, fine jade 20g, distilled water
1000mL；
(2) Purification is carried out to the pathogenic bacteria for being isolated from Panax notoginseng root and overground part, is accredited as Fusarium oxysporum
(Fusarium oxysporum), Fusarium solani (Fusarium solani), rotten mould (Pythium
Aphanidermatum), column spore mould (Cylindrocarpon destructans), botrytis cinerea (Botrytis are damaged
Cinerea), colletotrichum gloeosporioides Penz (Colletotrichum gloeosporioides) and Rhizoctonia solani Kuhn (Rhizoctonia
Solani), above-mentioned strain is seeded in PDA culture medium and is activated, after inoculation 3~4 times, take eugonic bacterial strain standby
With.
The chemical constituent of embodiment 3:GC-MS measurement lepidium seed volatile oil
(1) GC condition: instrument model: Agilengt Technologies 7890B-5977B；Chromatographic column: HP-5MS
30m×250μm×0.25μm；
(2) source EI source temperature: 230 DEG C；Level four bars temperature: 150 DEG C；Scanning range: 30~500m/z；Injection port temperature
Degree: 285 DEG C；Split ratio 10:1；Sample volume: 1 μ l；Electron energy: 70eV；Column oven temperature program: 50 DEG C of holding 4min, 5 DEG C/
Min is warming up to 120 DEG C, and 1 DEG C/min is warming up to 180 DEG C, and 10 DEG C/min is warming up to 280 DEG C of holding 16min；Wherein RI value is according to just
The retention time of structure alkane continuous carbon (C9-C25) is calculated, by the retention time of the compound and mass spectrum and NIST17.L data
Library carries out comparing, determines final compound, testing result see the table below；
Embodiment 4: the influence that measurement lepidium seed volatile oil grows 7 kinds of pathogenic bacteria mycelia in embodiment 2
(1) under aseptic technique, 15mL PDA culture medium is poured into each culture dish and takes reality after solidification to be cooled
The pathogenic bacteria for cultivating 7d in example 2 in PDA culture medium are applied, is beaten with the punch of diameter 5mm along colony edge and takes fungus block, be inoculated in
Culture medium central；
(2) 4 sterile Oxford cups are equidistantly placed at fungi block 25mm；
(3) DMSO and volumetric concentration of the lepidium seed volatile oil containing volumetric concentration 1% by embodiment 1 after dry
The aqueous dissolution of 0.1% Tween 80, it is 50mg/mL lepidium seed volatile oil liquid that concentration, which is made,；
(4) the 50mg/mL lepidium seed volatile oil liquid of 200 μ L steps (3) is added in each Oxford cup (in advance with 0.22
μm organic system filter filtration sterilization), 28 DEG C of constant temperature incubations in microbiological incubator contain body be not added lepidium seed volatile oil
The aqueous solution of the Tween 80 of the DMSO and volumetric concentration 0.1% of product concentration 1% is negative control, and hymexazol is positive control, often
A processing sets 5 repetitions；
(5) after cultivating 7d in incubator, colony diameter is measured using " crossing method "；
The result is shown in Figure 1,2, it can be seen that lepidium seed volatile oil has the antibacterial of wide spectrum to this 7 kinds of pathogens from Fig. 1,2
No matter effect and bacteriostasis rate height, all reached 100% to aerial part fungi or under ground portion fungi inhibiting rate.
Embodiment 5: lepidium seed volatile oil MIC measurement
(1) lepidium seed the volatile oil DMSO containing volumetric concentration 2% and volumetric concentration 1% by embodiment 1 after dry
Tween 80 aqueous dissolution, with multiple concentration that doubling dilution compound concentration range is 2500.00-1.22 μ g/mL ladder
Liquid is spent, it is 2500.00- that positive drug hymexazol, monomeric compound isothiocyanates aldehyde, which are configured to concentration range with same method,
The liquid of 1.22 μ g/mL；
(2) pathogen that 7d is grown in embodiment 2, (does not add agar, potato with 20mL 1/4PDA fluid nutrient medium
Amount with glucose is the 1/4 of conventional medium) culture medium is rinsed, it is 1 × 10 that spore concentration, which is made,4The spore suspension of a/mL
Liquid；
(3) the 50 μ L lepidium seed volatile oil and 150 μ L steps of 0.22 μm of filtering with microporous membrane degerming are added in 96 orifice plates
(2) manufactured spore suspension in；
(4) blank control be aqueous solution that 50 μ L contain 1% Tween 80 of volumetric concentration 2%DMSO and volumetric concentration and
150 μ L 1/4PDA fluid nutrient mediums；Positive control is that 50 μ L contain 1% Tween 80 of volumetric concentration 2%DMSO and volumetric concentration
Spore suspension in aqueous solution and 150 μ L steps (2)；
(5) 96 28 DEG C of orifice plate constant temperature incubation 36h, then fungi growing state in inspection hole；Microplate reader is used at 595nm
(Thermo 1510) measures the absorbance in each hole, as a result see the table below；
The result (mg/mL) of minimal inhibitory concentration (MIC)
Note: different letters indicate that different disposal has significant difference to the minimal inhibitory concentration of same bacterial strain in same row
p<0.05；
It will be seen that lepidium seed volatile oil is 0.27-0.7mg/mL to the MIC of 6 kinds of fungies from table；Hymexazol pair
The MIC of 6 kinds of fungies is 0.03-0.31mg/mL.
Embodiment 6: the live test of lepidium seed volatile oil
(1) lepidium seed volatile oil is added in the sterile culture matrix being made of vermiculite and quartz sand (volume ratio 2:1),
The culture substrate (0.1mg/g) containing volatile oil is obtained, valve bag is sealed；
(2) after fumigating the culture substrate containing volatile oil 5 days, basin is filled by 250 grams of every basin of amounts；
(3) with 1 × 107After the Fusarium oxysporum spore suspension of a spore/mL impregnates Radix Notoginseng seedling 3h, every potting kind 10
Strain, as lepidium seed volatilize oil processing group, negative control group be impregnate sterile water, while with without volatilization oil processing vermiculite and
The Radix Notoginseng seedling that the sterile culture matrix plantation spore suspension of quartz sand (volume ratio 2:1) composition impregnates is as positive control
Group；Every group of 5 repetitions；
(4) after cultivating 15 days, when apparent Disease symptoms occurs in positive controls seedling, to each group of Radix Notoginseng plant
Root system, stalk, the fresh weight of blade and dry weight and plant height measure, and calculate the disease incidence and disease index of each group, as a result
It see the table below and Fig. 3；
Note: different letters indicate that different disposal has significant difference to the minimal inhibitory concentration of same bacterial strain in same row
p<0.05；
As seen from the above table, lepidium seed volatilization oil processing group and negative control group in terms of disease incidence without significant difference but
With only be inoculated with F.oxysporum positive controls there are significant difference, illustrate using lepidium seed volatilize oil processing for
Radix Notoginseng disease caused by F.oxysporum has certain inhibiting effect.
Claims (3)
1. application of the lepidium seed volatile oil in prevention and treatment Chinese medicine or crop root rot.
2. application of the lepidium seed volatile oil in the pathogenic bacteria that prevention and treatment causes Chinese medicine or crop overground part that disease occurs.
3. application according to claim 2, it is characterised in that: pathogenic bacteria are botrytis cinerea (Botrytis cinerea), glue
Spore anthrax-bacilus (Colletotrichum gloeosporioides) and Rhizoctonia solani Kuhn (Rhizoctonia solani).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910715506.3A CN110326626B (en) | 2019-08-05 | 2019-08-05 | New application of semen lepidii volatile oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910715506.3A CN110326626B (en) | 2019-08-05 | 2019-08-05 | New application of semen lepidii volatile oil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110326626A true CN110326626A (en) | 2019-10-15 |
| CN110326626B CN110326626B (en) | 2021-05-14 |
Family
ID=68148714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910715506.3A Active CN110326626B (en) | 2019-08-05 | 2019-08-05 | New application of semen lepidii volatile oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110326626B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615705A (en) * | 2004-10-07 | 2005-05-18 | 刘伟平 | Plant source biological pesticide and producing method |
| CN106431507A (en) * | 2016-10-09 | 2017-02-22 | 西宁万润生物科技有限公司 | Method for extracting nutritional organic fertilizer from lepidium latifolium |
| CN106797965A (en) * | 2016-12-19 | 2017-06-06 | 湖北工程学院 | Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine |
| CN108669102A (en) * | 2018-06-26 | 2018-10-19 | 阜南县永兴工艺品有限公司 | A kind of preparation method of the disease control liquid of purple willow growth |
| WO2019012525A2 (en) * | 2017-07-09 | 2019-01-17 | Yeda Research And Development Co. Ltd. | Compositions comprising hydroxycinnamic acid and related conjugates, analogues and derivatives, and methods of use thereof |
-
2019
- 2019-08-05 CN CN201910715506.3A patent/CN110326626B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615705A (en) * | 2004-10-07 | 2005-05-18 | 刘伟平 | Plant source biological pesticide and producing method |
| CN106431507A (en) * | 2016-10-09 | 2017-02-22 | 西宁万润生物科技有限公司 | Method for extracting nutritional organic fertilizer from lepidium latifolium |
| CN106797965A (en) * | 2016-12-19 | 2017-06-06 | 湖北工程学院 | Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine |
| WO2019012525A2 (en) * | 2017-07-09 | 2019-01-17 | Yeda Research And Development Co. Ltd. | Compositions comprising hydroxycinnamic acid and related conjugates, analogues and derivatives, and methods of use thereof |
| CN108669102A (en) * | 2018-06-26 | 2018-10-19 | 阜南县永兴工艺品有限公司 | A kind of preparation method of the disease control liquid of purple willow growth |
Non-Patent Citations (5)
| Title |
|---|
| AHMED F. SAHAB 等: "Application of Some Plant Essential Oils to Control Fusarium Isolates Associated with Freshly Harvested Maize in Egypt", 《JOURNAL OF ESSENTIAL OIL BEARING PLANTS》 * |
| AL-ESSAWY,A.A. 等: "Effect of some essential oils on grey mould, caused by Botrytis cinerea on table grape at cold-storage", 《ARAB UNIVERSITIES JOURNAL OF AGRICULTURAL SCIENCES》 * |
| BAYSAL-GUREL, FULYA 等: "Effect of Organic Inputs and Solarization for the Suppression of Rhizoctonia solani in Woody Ornamental Plant Production", 《PLANTS-BASEL》 * |
| HUMAIRA RIZWANA: "In vitro antibacterial and antifungal activity of some oils, chemical analysis and their FTIR studies", 《INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY》 * |
| 主编：黄璐琦: "《道地药材品质保障技术研究》", 31 January 2018, 上海科学技术出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110326626B (en) | 2021-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hashem et al. | Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp. | |
| CN110352989A (en) | The new application of purple perilla volatile oil | |
| CN105494460B (en) | Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest | |
| CN105039167B (en) | A kind of beauveria bassiana DSXJ 07 and its application | |
| CN109287677A (en) | The purposes of flos caryophylli volatile oil | |
| CN104754945B (en) | For the composition containing parasitics, pathogenic or weeds biosystem nucleic acid for suppressing and/or controlling biosystem to grow | |
| CN106172506A (en) | A kind of prevention or the method for the treatment of banana Panama disease | |
| CN110250218A (en) | The new application of Herba Menthae Haplocalycis volatile oil | |
| CN116210728B (en) | Bactericide and preparation method and application thereof | |
| Geösel et al. | Effect of essential oils on mycopathogens of Agaricus bisporus. | |
| CN109452320A (en) | The purposes of Fructus Caryophylli volatile oil | |
| CN101502275A (en) | Plant source pesticide | |
| CN110269069B (en) | New application of fennel volatile oil | |
| CN104782675B (en) | Novel plant disease biocide and preparation method thereof | |
| CN110169422A (en) | A kind of combined dosage form bactericide and its preparation method and application | |
| CN110326626A (en) | The new application of lepidium seed volatile oil | |
| CN110250215A (en) | The new application of volatile oil of Herba Eupatorii | |
| CN106857113A (en) | A kind of method for shortening watermelon period of crop-rotation prevention and control watermelon blight | |
| CN107751252B (en) | Application of Ledum palustre branch and leaf total volatile oil in prevention and control of stored grain pests | |
| CN110250219B (en) | New application of patchouli volatile oil | |
| CN110250216A (en) | The new application of Herba laggera volatile oil | |
| CN109566660A (en) | A kind of application of Lavender | |
| CN105106278A (en) | Chinese herbal medicine sterilization and disinfection lotion and preparation method thereof | |
| CN109247347A (en) | The purposes of Fructus Citri Sarcodactylis volatile oil | |
| CN114903040B (en) | Application of Menthone in Tea Tree Disease Control |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |