CN110169422A - A kind of combined dosage form bactericide and its preparation method and application - Google Patents
A kind of combined dosage form bactericide and its preparation method and application Download PDFInfo
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- CN110169422A CN110169422A CN201910291438.2A CN201910291438A CN110169422A CN 110169422 A CN110169422 A CN 110169422A CN 201910291438 A CN201910291438 A CN 201910291438A CN 110169422 A CN110169422 A CN 110169422A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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Abstract
The present invention relates to a kind of combined dosage form bactericides, are made in mass ratio of following raw material: false stinkweed cauline leaf: ginkgo leaf: cordate houttuynia: radix scutellariae: Radix Isatidis: Fructus Forsythiae: Herba Andrographitis: compound sodium nitrophenolate: OP-10 emulsifier: false stinkweed flower essential oil: water=20~40:2~5:4~16:2~6:3~8:2~8:2~8:1.0~2.5:0.01~0.03:0.1~0.25:10~30.The present invention uses based on natural plant resource, the opacifiers prepared supplemented by strength cell-activating agent-compound sodium nitrophenolate, and the present invention has the characteristics that green, environmentally friendly, nontoxic, plant absorption utilization rate is high, is subject to rationalization utilization to instruction plant to the maximum extent.Plant origin of the present invention is easy to get, and preparation process is relatively simple, and cost is not high, is suitble to agricultural/forestry industrialization production；Bactericide of the invention has stronger killing ability to Ralstonia solanacearum, citrus sand skin disease, also has depression effect to a certain degree to yellow twig pathogen.
Description
Technical field
The invention belongs to bactericide technical fields, and in particular to a kind of plant compound bactericide and preparation method thereof and
Using.
Background technique
China's plant resources very abundant, only the kind number of medicinal plant is just up to more than 8000 kinds, and wherein quite a few has
Various sterilization active constituent, including terpene, alkaloids, amides, long-chain carbon alkane and fatty acid, nitrogenous sulfur-containing compound
Class, aromatics species, phenols, flavonoids and aromatic compound etc..As false stinkweed alcohol extract can reduce on citrus seedling young sprout
The egg laying amount of diaphorina citri, essential oil have repellent action to diaphorina citri adult；The alcohol extract of cordate houttuynia is to hydroxyl radical free radical
(OH), superoxide radical (O2 ?) and nitrite (NO2 ?) elimination effect it is significant, and to various bacteria and some fungi disease
Opportunistic pathogen has inhibition and killing effect；Contain more than 30 chromocor compounds and terpene, phenols, microelement and amino acid in ginkgo leaf
Etc. effective components, flavones crude extract is to bacterium (staphylococcus aureus, Escherichia coli) and fungi (Fusarium graminearum, tobacco
Brown spot pathogen) there is certain fungistatic effect；The extract of Radix Isatidis, which has bacterium and virus, to be inhibited and kills to make well
With；Fructus Forsythiae, Herba Andrographitis etc. have good inhibiting effect to pathogenetic bacteria；It is provided using these plants with natural disinfection effect
It is developed novel, green in conjunction with the compound efficient, safe, cheap and easy to get of some enhancing stress resistance of plant having found in source
Color, noresidue, free of contamination environmentally friendly fungicide are imperative.
Summary of the invention
The present invention i.e. in summary sterilize in restraining and sterilizing bacteria component difference and to different genera pathogenic microorganism by plant resources
Advantage in terms of effect develops a kind of combined dosage form bactericide.The bactericide has Ralstonia solanacearum, citrus sand skin disease
Stronger killing ability also has depression effect to a certain degree to yellow twig pathogen；The present invention is nontoxic to the human body, and dosage form is milkiness
Liquid, use is safe, sprays rear plant and easily absorbs, no pollution to the environment, has stronger application value.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of plant compound bactericide, by following original
Material is made in mass ratio: false stinkweed cauline leaf: ginkgo leaf: cordate houttuynia: radix scutellariae: Radix Isatidis: Fructus Forsythiae: Herba Andrographitis: compound sodium nitrophenolate: OP-
10 emulsifiers: false stinkweed flower essential oil: water=20~40:2~5:4~16:2~6:3~8:2~8:2~8:1.0~2.5:
0.01~0.03:0.1~0.25:10~30.
In addition, the present invention also provides a kind of methods for preparing above-mentioned bactericide, method includes the following steps:
(1) collect false stinkweed, will flower it is separated with cauline leaf, dried in shady place, then colored to be placed in evaporation-cold by false stinkweed
In solidifying container/equipment, the solid-liquid ratio of 1:20 adds water in mass ratio, and condensate liquid is collected in 100 DEG C of evaporations, as false stinkweed flower essential oil,
It is spare.
(2) first plant material is shredded, ginkgo leaf, cordate houttuynia and false stinkweed cauline leaf are cut into 1~2cm strip, other wooden
Raw material is cut into 1cm or so stem section, pours into reaction kettle after mixing in proportion, adds 15~20 times of plant material quality of water to stir and mentions
It takes, 80~85 DEG C of Extracting temperature, extraction time is 1.5~2.0h；Second of extraction is carried out after filtering again, amount of water is initial plants
8~12 times of raw material quality, Extracting temperature control is at 80~85 DEG C, and extraction time is 1h~1.5h, merging filtrate after filtration,
100 DEG C of evaporation and concentration are warming up to, until the 28~35% of plant material total weight.
(3) 95% ethyl alcohol is added in Xiang Shangshu concentrate, until the mass percentage of ethyl alcohol is 60%~70%, stirring is equal
Even standing 16 afterwards~for 24 hours, take supernatant to rotary evaporation devices, steaming ethyl alcohol to the greatest extent obtains half solidification state/sticky mass, further according to
Its quality adds water in the ratio of 1:0.8~1.2, continues to add 0.1~0.25 vacation by plant material mass ratio after mixing
Stinkweed flower essential oil, 1.0~2.5 compound sodium nitrophenolate, 0.01~0.03 OP-10 emulsifier, after the abundant homogeneous of homogenizer to obtain the final product
Plant compound bactericide.
The usage and dosage of bactericide of the invention are as follows: plant protection product dosage 0.05kg~0.3kg per acre, when use
Plant protection product is first diluted with water 200~300 times, then by the bactericide even spraying after dilution on plant.It is right
Ralstonia solanacearum, citrus sand skin germ have stronger killing ability, also have depression effect to a certain degree to yellow twig pathogen.
Compared with the prior art, the present invention has the following advantages:
1, bactericide of the invention uses based on natural plant resource, with strength cell-activating agent-compound sodium nitrophenolate
Supplemented by the opacifiers for preparing, the present invention has the characteristics that green, environmentally friendly, nontoxic, plant absorption utilization rate is high.
2, bactericide of the invention is not only to eliminate instruction plant using China instruction plant false stinkweed as primary raw material
A kind of new thinking is provided, can also turn waste into wealth, rationalization utilization is subject to instruction plant to the maximum extent.
3, through detecting, the ingredients such as volatile spirits, polysaccharide, flavones and alkaloid are contained in bactericide of the invention,
The characteristic that middle polysaccharide has protection immune, volatile spirits, flavones have the characteristic of antibacterial pest expelling, and alkaloid has the function of sterilization.
4, bactericide of the invention has stronger killing ability to Ralstonia solanacearum, citrus sand skin germ, to yellow twig
Pathogen also has depression effect to a certain degree；The present invention is nontoxic to the human body, and dosage form is emulsion, and use is safe, sprays rear plant
It easily absorbs, no pollution to the environment, there is stronger application value.
Specific embodiment
Below by embodiment, technical scheme of the present invention will be described in further detail.
Embodiment 1
A kind of plant compound bactericide of the present embodiment, is made in mass ratio of following raw material: false stinkweed cauline leaf: ginkgo
Leaf: cordate houttuynia: radix scutellariae: Radix Isatidis: Fructus Forsythiae: Herba Andrographitis: compound sodium nitrophenolate: OP-10 emulsifier: false stinkweed flower essential oil: water=30:2:
12:4:5:4:4:1.5:0.02:0.2:25.
The preparation method of the plant protection product of the present embodiment the following steps are included:
(1) collects false stinkweed, and flower is separated with cauline leaf, dries in shady place, then false stinkweed flower is placed in evaporation-
In condensing container/equipment, the solid-liquid ratio of 1:20 adds water in mass ratio, and condensate liquid is collected in 100 DEG C of evaporations, as false stinkweed anthosin
Oil, it is spare.
(2) first shreds plant material, and ginkgo leaf, cordate houttuynia and false stinkweed cauline leaf are cut into 1~2cm strip, other wooden
Raw material is cut into 1cm or so stem section, pours into reaction kettle after mixing in proportion, adds 18 times of water to stir and extracts, and Extracting temperature 80~
85 DEG C, extraction time 2.0h；Second of extraction is carried out after filtering again, amount of water is 10 times of initial feed quality, extracts temperature
At 80~85 DEG C, extraction time 1.5h, merging filtrate after filtration is warming up to 100 DEG C of evaporation and concentration, until plant material for degree control
The 28~35% of total weight.
(3) 95% ethyl alcohol is added into above-mentioned concentrate by, until alcohol content is 70%, stands for 24 hours, takes after mixing evenly
Clearly to rotary evaporation devices, ethyl alcohol to the greatest extent is steamed, solid sticky mass is partly coagulated, added further according to its quality in the ratio of 1:1
Add water, continues to add 0.2 false stinkweed flower essential oil, 1.5 compound sodium nitrophenolate, 0.02 by plant material mass ratio after mixing
OP-10 emulsifier, up to the present invention after the abundant homogeneous of homogenizer.
The plant protection product of 0.3kg the present embodiment is diluted with water 200 times, and the citrus plant that bacterial wilt is infected 1 mu is equal
Even spray pesticide is administered 30 days bacterial wilts and is effectively prevented, solves the harm of citrus bacterial wilt.
Embodiment 2
The plant protection product of the present embodiment is made in mass ratio of following raw material: false stinkweed cauline leaf: ginkgo leaf: cordate houttuynia:
Radix scutellariae: Radix Isatidis: Fructus Forsythiae: Herba Andrographitis: compound sodium nitrophenolate: OP-10 emulsifier: false stinkweed flower essential oil: water=40:3:10:5:4:6:
6:2.0:0.025:0.2:20.
The preparation method of the plant protection product of the present embodiment the following steps are included:
(1) collects false stinkweed, and flower is separated with cauline leaf, dries in shady place, then false stinkweed flower is placed in evaporation-
In condensing container/equipment, the solid-liquid ratio of 1:20 adds water in mass ratio, and condensate liquid is collected in 100 DEG C of evaporations, as false stinkweed anthosin
Oil, it is spare.
(2) first shreds plant material, and ginkgo leaf, cordate houttuynia and false stinkweed cauline leaf are cut into 1~2cm strip, other wooden
Raw material is cut into 1cm or so stem section, pours into reaction kettle after mixing in proportion, adds 18 times of water to stir and extracts, and Extracting temperature 80~
85 DEG C, extraction time 2.0h；Second of extraction is carried out after filtering again, amount of water is 10 times of initial feed quality, extracts temperature
At 80~85 DEG C, extraction time 1.5h, merging filtrate after filtration is warming up to 100 DEG C of evaporation and concentration, until plant material for degree control
The 28~35% of total weight.
(3) 95% ethyl alcohol is added into above-mentioned concentrate by, until alcohol content is 70%, stands for 24 hours, takes after mixing evenly
Clearly to rotary evaporation devices, ethyl alcohol to the greatest extent is steamed, solid sticky mass is partly coagulated, added further according to its quality in the ratio of 1:1
Add water, continues to add 0.2 false stinkweed flower essential oil, 2.0 compound sodium nitrophenolate, 0.025 by plant material mass ratio after mixing
OP-10 emulsifier, up to the present invention after the abundant homogeneous of homogenizer.
The plant protection product of 0.35kg the present embodiment is diluted with water 300 times, and the citrus plant that husky skin disease is infected 1 mu is equal
Even spray pesticide, being administered 30 days husky skin disease germs is inhibited, and shows that the bactericide of the present embodiment has citrus sand skin disease
Good control efficiency.
Restraining and sterilizing bacteria effect of the bactericide of the present invention to Ralstonia solanacearum, citrus sand skin pathogen and yellow twig pathogen:
One, by taking the bactericide of embodiment 1 as an example, bactericide of the present invention is measured to the indoor virulence of Ralstonia solanacearum:
It is respectively by plant protection product concentration is hybridly prepared into the plant protection product addition PDA culture solution of embodiment 1
The sample of 18.75 μ g/g, 37.50 μ g/g, 75.00 μ g/g, 150 μ g/g, 750 μ g/g, 1500 μ g/g and 3000 μ g/g, after sterilizing
The PDA culture medium of the plant protection product containing various concentration is obtained, and using PDA culture medium as control；By purified Ralstonia solanacearum
It is inoculated on TTC culture medium, is placed in culture in 37 DEG C of incubators, makes bacteria cake from colony edge with the punch that diameter is 0.4cm
It is several, it is spare；Bacteria cake is reversely transplanted to the TTC culture medium of PDA culture medium and the bactericide containing various concentration with transfer needle
On, 3 bacteria cakes of every ware, every processing sets secondary repetition, is placed in 37 DEG C of incubators and cultivates for 24 hours afterwards with the measurement microscope bacterium with scale
Diameter is fallen, the data obtained is handled with EXCEL software, obtains relative inhibition and probit value, using log concentration as abscissa (X),
Using probit value as ordinate (Y), through scatterplot regression analysis, regression equation is obtained, when y=0 acquires the log concentration of EC50
Value, the results are shown in Table 1.
1 bactericide of table is to Ralstonia solanacearum The Toxicity Determination result
As shown in Table 1, bactericide of the invention is 207.04 μ g/g to the EC50 of Ralstonia solanacearum, and virulence regression equation is
It is stronger to show that bactericide of the invention has Ralstonia solanacearum bacterium for Y=1.3219x-3.0616, coefficient R=0.987
Inhibitory activity.
Two, by taking the bactericide of embodiment 1 as an example, bactericide of the present invention is measured to citrus sand skin disease pathogen
Indoor virulence:
The bactericide of embodiment 1 is configured to 18.75 μ g/g, 37.50 μ g/g, 75.00 μ g/g, 150 μ g/g, 375 μ
G/g, 750 μ g/g and 1500 μ g/g, seven concentration gradient solution scrape citrus sand skin disease leaf back sporangium into it after sterilizing
It is interior, and the citrus sand skin disease sporangium that equivalent is added in sterile water compares.Moisturizing is trained in big culture dish at room temperature
After supporting for 24 hours, spore count and total spore count are sprouted in field of view under the microscope, the data obtained is handled with EXCEL software, obtains
Relative inhibition and probit value, using log concentration as abscissa (X), using probit value as ordinate (Y), through scatterplot regression analysis,
Obtain regression equation, when y=0 acquires the log concentration value of EC50, and the results are shown in Table 2.
It corrects inhibiting rate=(blank control sprouts spore count-sample sets and sprouts spore count)/blank control and sprouts spore count
2 bactericide of table is to citrus sand skin disease pathogen The Toxicity Determination result
As shown in Table 2, plant protection product of the invention is 34.77 μ g/g, virulence to the EC50 of citrus sand skin disease pathogen
Regression equation is Y=1.0995x-1.6946, and coefficient R=0.9334 shows plant protection product of the invention to citrus sand
Skin disease pathogen has stronger inhibitory activity.
Three, by taking the bactericide of embodiment 1 as an example, bactericide of the present invention is measured to citrus huanglongbing pathogen bacterium
Control efficiency
The mandarin tree for obviously infecting yellow twig is chosen, the newborn blade of 30d or so is taken, Liquid nitrogen storage sprays this plant and kills
200 times of dilutions of microbial inoculum take after 20d again and have picked antimere blade, and every group 3 parallel.Again with citrus huanglongbing pathogen Asia
Continent fungus strain β-operon special primer fA2/rJ5 carries out PCR amplification, analyzes through fluorescence quantitative PCR detection, first obtains yellow twig
Fluorogenic quantitative detection standard curve goes out bacteria containing amount by regression equation calculation, and acquired results are as shown in table 3 below:
Control efficiency of 3 bactericide of table to citrus huanglongbing pathogen bacterium
Note: every group three parallel
As shown in Table 3, bactericide of the invention to the 20d inhibiting rate of citrus huanglongbing pathogen bacterium up to 77.02%,
Though citrus huanglongbing pathogen bacterium cannot be killed completely, but still show stronger rejection ability, shows plant sterilization of the invention
Agent has preferable inhibitory activity to citrus huanglongbing pathogen bacterium.
To sum up, plant origin of the present invention is easy to get, and preparation process is relatively simple, and cost is not high, is suitble to agricultural/forestry
Industrialization production；Bactericide of the invention has stronger killing ability to Ralstonia solanacearum, citrus sand skin disease, to yellow twig disease
Opportunistic pathogen also has depression effect to a certain degree；The present invention is nontoxic to the human body, and dosage form is emulsion, and use is safe, and it is easy to spray rear plant
It absorbs, no pollution to the environment, there is stronger application value.
The above is only presently preferred embodiments of the present invention, not does any restrictions to the present invention, all according to invention skill
Art any simple modification substantially to the above embodiments, change and equivalent structural changes, still fall within the technology of the present invention
In the protection scope of scheme.
Claims (4)
1. a kind of plant compound bactericide, which is characterized in that be made in mass ratio of following raw material: false stinkweed cauline leaf: ginkgo leaf:
Cordate houttuynia: radix scutellariae: Radix Isatidis: Fructus Forsythiae: Herba Andrographitis: compound sodium nitrophenolate: OP-10 emulsifier: false stinkweed flower essential oil: water=20~40:2
~5:4~16:2~6:3~8:2~8:2~8:1.0~2.5:0.01~0.03:0.1~0.25:10~30.
2. a kind of preparation method of plant compound bactericide according to claim 1, which is characterized in that including following step
It is rapid:
(1) false stinkweed is collected, flower is separated with cauline leaf, it is dried in shady place, then false stinkweed flower is placed in evaporation-condensation and is held
In device/equipment, the solid-liquid ratio of 1:20 adds water in mass ratio, and 100 DEG C of evaporations collect condensate liquid, obtain false stinkweed flower essential oil；
(2) first plant material is shredded, ginkgo leaf, cordate houttuynia and false stinkweed cauline leaf are cut into 1~2cm strip, other wood materials
It is cut into 1cm stem section, is poured into reaction kettle after mixing in proportion, adds 15~20 times of plant material quality of water to stir and extracts, extract
80~85 DEG C of temperature, extraction time is 1.5~2.0h；Second of extraction is carried out after filtering again, amount of water is original plant raw material
8~12 times of quality, at 80~85 DEG C, extraction time is 1h~1.5h for Extracting temperature control, and merging filtrate after filtration is warming up to
100 DEG C of evaporation and concentration, until the 28~35% of plant material total weight；
(3) 95% ethyl alcohol is added into step (2) resulting concentrate, until ethyl alcohol mass percentage is 60%~70%, stirs
Stand 16 after mixing uniformly~for 24 hours, it takes supernatant to rotary evaporation devices, steams ethyl alcohol to the greatest extent, obtain half solidification state/sticky mass, then
Water is added in the ratio of 1:0.8~1.2 according to its quality, continues to add 0.1~0.25 by plant material mass ratio after mixing
False stinkweed flower essential oil, 1.0~2.5 compound sodium nitrophenolate, 0.01~0.03 OP-10 emulsifier, after the abundant homogeneous of homogenizer
Up to plant compound bactericide.
3. a kind of application of plant compound bactericide according to claim 1, which is characterized in that for kill Ralstonia solanacearum,
Citrus sand skin germ.
4. a kind of application of plant compound bactericide according to claim 1, which is characterized in that for inhibiting yellow twig sick
Opportunistic pathogen.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624527A (en) * | 2018-05-12 | 2018-10-09 | 湖南科技学院 | A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt |
| CN111567525A (en) * | 2020-05-08 | 2020-08-25 | 易之泰生物科技（龙岩）有限公司 | Composition and method for preventing and treating citrus greening disease |
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