CN108624527A - A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt - Google Patents
A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The present invention relates to biotechnology, the ginkgo source growth-promoting preparation of specifically a kind of prevention ginger bacterial wilt is made of the raw material of following parts by weight：5.0 10.0 parts of 0.8 1.2 parts of mocha husband bacillus, 0.6 1.0 parts of fusarium prolifertum, 7 0.3 0.5 parts of the gingko endogenous fungus fusarium solani T with broad spectrum antibiotic activity and distilled water.The invention further relates to a kind of preparation method and applications of the ginkgo source growth-promoting preparation of prevention ginger bacterial wilt.The present invention is specific to the biological prevention and control agent of ginger bacterial wilt exploitation.Due to being biological agent, not no a series of problems caused by the use because of chemical pesticide completely, thus be conducive to the No-harmful apple orchard of vegetables, peasant can not have to or reduce the dosage of other chemical pesticides, this can not only reduce expenses for peasant, and be conducive to the outlet of vegetables, meanwhile, reduce the diseased plant rate of ginger, 10% or less is dropped to by original 20 40%, it can be that peasant increases income, reduce cost, improve economic value.
Description
Technical field
The present invention relates to biotechnology, the ginkgo source growth-promoting preparation of specifically a kind of prevention ginger bacterial wilt.
The invention further relates to a kind of preparation method and applications of the ginkgo source growth-promoting preparation of prevention ginger bacterial wilt.
Background technology
Ginger bacterial wilt is commonly called as " bacterial wilt of ginger ", is caused by Ralstonia solanacearum (Ralstonia solanacearum)
A kind of bacterial disease.This soil-borne pathogen is distributed mainly on tropical and subtropical zone, and host range is very extensive, can infect
300 various crop of a section more than 50.The pathogen mainly by soil-borne, root infect after in vascular bundle mass propagation cause to tie up
The blocking of tube bank influences the Water Transportation of host crop, dead to cause to wilt.This disease is due to geographic range
Extensively, host range is wide, is very easy to cause crushing blow so once infecting, up to the present there are no effectively preventing hands
Section.
Ginger is the important industrial crops in China, and bacterial wilt of ginger had both had been reported that in the 1950s, in recent years incidence trend
Gradually serious, according to investigations, the general diseased plant rate in field is 10%~20%, and grave illness field is up to 30%~40%, and local plot is very
It loses and receives to total crop failure.And since morbidity reduces quality, cause serious economic loss.
For a long time, both at home and abroad to anti-Zhiduo County of bacterial wilt of ginger from cultural control and chemical prevention, control effect is unknown
It is aobvious, and cause a degree of environmental pollution.Therefore, biological control the advantages of its own due to starting by more and more
Attention.In conclusion exploitation it is new, highly efficient and safe microbial bactericide control bacterial wilt of ginger be to improve society
The needs of meeting production, while the needs of even more agricultural safety sustainable development, symbol social development demand.
Invention content
Technical problem to be solved by the present invention lies in overcome the deficiencies of the prior art and provide a kind of prevention ginger bacterial wilt
Ginkgo source growth-promoting preparation.
In order to solve the above technical problems, the present invention now proposes following technical scheme：A kind of ginkgo source of prevention ginger bacterial wilt
Growth-promoting preparation is made of the raw material of following parts by weight：0.8-1.2 parts of mocha husband bacillus, 0.6-1.0 parts of fusarium prolifertum,
5.0-10.0 parts of 0.3-0.5 parts of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity and distilled water.
Further, it is made of the raw material of following parts by weight：1.0 parts of mocha husband bacillus, 0.8 part of fusarium prolifertum,
7 parts of 0.4 part of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity and distilled water.
Further, the cultural method of the mocha husband bacillus is：First by mocha husband bacillus in seed culture
Culture to strain density OD600 values are 0.6-0.8 in base, bacterium solution are obtained, then in 100 parts by weight inoculation of medium 2-5 weight
Part mocha husband bacillus bacterial strain, 37 DEG C culture, until mocha husband bacillus viable count be 2-20 × 207/gram
Culture medium.
Further, the formula of the seed culture medium is：Beef extract 3g, peptone 10g, sodium chloride 5g, agar 15-
20g, distilled water 1000mL, pH value 7.0-7.2,121 DEG C of sterilizing 30min；The formula of the 100 parts by weight culture medium is:Grape
Sugared 15g, starch 1g, beancake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride
0.1g, calcium carbonate 0.1g, pH7.0-7.2.
Further, the cultural method of the fusarium prolifertum is：First fusarium prolifertum is cultivated in PDA culture medium to
Strain density OD600 values are 0.6-0.8, obtain bacterium solution, then go out sickle in the layer of 100 parts by weight inoculation of medium 2-5 parts by weight
The bacterial strain of knife bacterium, 37 DEG C culture, until fusarium prolifertum viable count be 2-20 × 207/gram culture medium；The bacterium is in PDA
Average growth rate on culture medium is 7mm/d, covers with entire tablet within 12 days.Medium matrix reverse side is buff, and bacterium colony is in
White, fluffy protrusion, flocculence are intensive.Mycelia microscopic morphology (400 ×)：Mycelia separates clear and more, 3-5 μm of diameter, point
Raw sporophore is more straight, and top is in sausage shape, has no apparent spore.Thalli morphology in zymotic fluid：Bacterium solution is slightly muddy, bacterium in zymotic fluid
Silk is white cloud, and liquid level has no bubble.It generates mycotoxin and inhibits certain plants growth；Fermentation generates antitumor activity medicine
Object spherosin (swainsonine), tunning ethyl acetate polarity section possess good bacteriostatic activity.
Further, the ingredient of the PDA culture medium is：Distilled water 1000mL, potato 200g, agar powder 20g, grape
Sterilize 20min under sugared 20g, 121 DEG C of 2.4 × 105Pa of air pressure；The formula of the 100 parts by weight culture medium is:Glucose 15g forms sediment
Powder 1g, beancake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, carbonic acid
Calcium 0.1g, pH7.0-7.2.
Further, the cultural method of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity is：
The mycelia of inclined-plane culture is connected to 25-35 DEG C of PDA solid plates to cultivate 72-120 hours, then is forwarded to PDA liquid medium 25-
35 DEG C activate 48-72 hours, 3-10% inoculum concentrations by volume, in liquid culture medium of transferring, pH to 6-9, and 25-35 DEG C
Under, it cultivates 72 hours to 120 hours.
Further, the ingredient of the PDA solids and fluid nutrient medium is：Distilled water 1000mL, potato 200g, agar
Sterilize 20min under powder 20g, glucose 20g, 121 DEG C of 2.4 × 105Pa of air pressure；The liquid culture medium is：Content 50%
Remaining is water by Gardenoside 20g/L, NaNO33g/L, KH2PO440g/L, with NaOH tune pH to 7.5.
It is another object of the present invention to additionally provide a kind of preparation side of the ginkgo source growth-promoting preparation of prevention ginger bacterial wilt
Method, include the following steps：(1) it is 0.6- to cultivate mocha husband bacillus to strain density OD600 values in seed culture medium
0.8, bacterium solution is obtained, then in the bacterial strain of the mocha husband bacillus of 100 parts by weight inoculation of medium 2-5 parts by weight, 37 DEG C
Culture, until mocha husband bacillus viable count be 2-20 × 207/gram culture medium；(2) by fusarium prolifertum in PDA
Culture to strain density OD600 values are 0.6-0.8 in culture medium, bacterium solution are obtained, then in 100 parts by weight inoculation of medium 2-5
The bacterial strain of the fusarium prolifertum of parts by weight, 37 DEG C culture, until fusarium prolifertum viable count be 2-20 × 207/gram training
Support base；(3) the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity of inclined-plane culture PDA solids are connected to put down
25-35 DEG C of plate is cultivated 72-120 hours, then is forwarded to activation 48-72 hours of 25-35 DEG C of PDA liquid medium, by volume 3-
10% inoculum concentration, in liquid culture medium of transferring, pH to 6-9 at 25-35 DEG C, is cultivated 72 hours to 120 hours；(4) to
Distilled water is added in container according to the ratio, at room temperature sealing a period of time；(5) at room temperature, mocha is then added according to the ratio successively
Husband bacillus, fusarium prolifertum and the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity, concussion is uniform,
.
Another object of the present invention is application of the growth-promoting preparation in preventing ginger bacterial wilt.
Compared with prior art, the beneficial effects of the present invention are：1, the present invention is specific to ginger bacterial wilt and opens
The biological prevention and control agent of hair.Due to being biological agent, not no a series of problems caused by the use because of chemical pesticide completely, thus have
Conducive to the No-harmful apple orchard of vegetables, peasant can not have to or reduce the dosage of other chemical pesticides, this can be not only that peasant saves
Spending, and is conducive to the outlet of vegetables, meanwhile, reduce the diseased plant rate of ginger, by original 20-40% drop to 10% with
Under, it can be that peasant increases income, reduce cost, improve economic value；
2, growth-promoting preparation production method of the invention is simple, highly practical.
Specific implementation mode
, there are further understanding and understanding in the effect of to make to structure feature of the invention and being reached, to preferable
Embodiment is described in detail, and is described as follows：
Embodiment 1
A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt, is made of the raw material of following parts by weight：Mocha husband brood cell's bar
0.8 part of bacterium, 0.6 part of fusarium prolifertum, 0.3 part of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity and steaming
5.0 parts of distilled water.
Embodiment 2
A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt, is made of the raw material of following parts by weight：Mocha husband brood cell's bar
1.0 parts of bacterium, 0.8 part of fusarium prolifertum, 0.4 part of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity and steaming
7 parts of distilled water.
Embodiment 3
A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt, is made of the raw material of following parts by weight：Mocha husband brood cell's bar
1.2 parts of bacterium, 1.0 parts of fusarium prolifertum, 0.5 part of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity and steaming
10.0 parts of distilled water.
Embodiment 4
A kind of preparation method of the ginkgo source growth-promoting preparation of prevention ginger bacterial wilt, includes the following steps：(1) by mocha husband's bud
It is 0.7 that spore bacillus, which is cultivated in seed culture medium to strain density OD600 values, bacterium solution is obtained, then in 100 parts by weight culture mediums
It is inoculated with the bacterial strain of the mocha husband bacillus of 2-5 parts by weight, 37 DEG C of cultures, until the viable count of mocha husband bacillus is 2-20
The culture medium of × 207/gram；(2) it is 0.7 to cultivate fusarium prolifertum in PDA culture medium to strain density OD600 values, is obtained
Bacterium solution, then in the bacterial strain of the fusarium prolifertum of 100 parts by weight inoculation of medium 2-5 parts by weight, 37 DEG C of cultures, until layer goes out
The viable count of sickle-like bacteria be 2-20 × 207/gram culture medium；(3) by the ginkgo with broad spectrum antibiotic activity of inclined-plane culture
Endogenetic fungus fusarium solani T-7 is connected to 30 DEG C of PDA solid plates and cultivates 90 hours, then is forwarded to 30 DEG C of PDA liquid medium
Activation 60 hours, 7% inoculum concentration by volume, in liquid culture medium of transferring, pH to 8 at 30 DEG C, is cultivated 100 hours；
(4) distilled water is added according to the ratio into container, at room temperature sealing a period of time；(5) at room temperature, it then adds according to the ratio successively
Mocha husband bacillus, fusarium prolifertum and the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity, concussion are equal
It is even, you can.
Further, in the present embodiment, the cultural method of mocha husband bacillus is：First mocha husband bacillus is being planted
Culture to strain density OD600 values are 0.6-0.8 in sub- culture medium, bacterium solution are obtained, then in 100 parts by weight inoculation of medium 2-
The bacterial strain of the mocha husband bacillus of 5 parts by weight, 37 DEG C of cultures, until the viable count of mocha husband bacillus is 2-20 × 207
It is a/gram culture medium；Wherein, the formula of seed culture medium is：Beef extract 3g, peptone 10g, sodium chloride 5g, agar 15-20g,
Distilled water 1000mL, pH value 7.0-7.2,121 DEG C of sterilizing 30min；The formula of 100 parts by weight culture mediums is:Glucose 15g forms sediment
Powder 1g, beancake powder 25g, manganese sulfate 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, carbonic acid
Calcium 0.1g, pH7.1.
Further, in the present embodiment, the cultural method of fusarium prolifertum is：First by fusarium prolifertum in PDA culture medium
Middle culture to strain density OD600 values are 0.6-0.8, bacterium solution are obtained, then in 100 parts by weight inoculation of medium 2-5 parts by weight
Fusarium prolifertum bacterial strain, 37 DEG C culture, until fusarium prolifertum viable count be 2-20 × 207/gram culture medium；Its
In, the ingredient of PDA culture medium is：Distilled water 1000mL, potato 200g, agar powder 20g, glucose 20g, 121 DEG C of air pressures 2.4
Sterilize 20min under × 105Pa；The formula of 100 parts by weight culture mediums is:Glucose 15g, starch 1g, beancake powder 25g, manganese sulfate
1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, calcium carbonate 0.1g, pH7.0-7.2.
Further, in the present embodiment, the culture of the gingko endogenous fungus fusarium solani T-7 with broad spectrum antibiotic activity
Method is：The mycelia of inclined-plane culture is connected to 30 DEG C of PDA solid plates to cultivate 90 hours, then is forwarded to PDA liquid medium 30
DEG C activation 60 hours, 6.5% inoculum concentration by volume, in liquid culture medium of transferring, pH to 8, at 30 DEG C, culture 100
Hour.
Test example
By test example as can be seen that each ingredient of the growth-promoting preparation of the present invention has promotion to make prevention ginger bacterial wilt
With, but after combining the promotion preparation for forming the present invention, ginger diseased plant rate is lower, and survival rate higher, effect is more preferable,
And can show that the proportioning facilitation of each ingredient of embodiment 2 is best, ginger diseased plant rate is only 7%.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than to limit it；
Although the present invention is described in detail referring to the foregoing embodiments, it will be understood by those of skill in the art that it still may be used
To modify to the technical solution that previous embodiment is recorded or equivalent replacement of some of the technical features；And this
A little modifications or substitutions, the spirit and scope for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt, which is characterized in that be made of the raw material of following parts by weight：Mocha
0.8-1.2 parts of husband bacillus, 0.6-1.0 parts of fusarium prolifertum, the gingko endogenous fungus Beancurd sheet sickle with broad spectrum antibiotic activity
5.0-10.0 parts of 0.3-0.5 parts of spore bacterium T-7 and distilled water.
2. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 1, which is characterized in that by following heavy
Measure the raw material composition of part：1.0 parts of mocha husband bacillus, 0.8 part of fusarium prolifertum, with the gingko endogenous of broad spectrum antibiotic activity
7 parts of 0.4 part of fungi fusarium solani T-7 and distilled water.
3. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 1, which is characterized in that the mocha
The cultural method of husband bacillus is：First mocha husband bacillus is cultivated in seed culture medium to strain density OD600 values and is
0.6-0.8 obtains bacterium solution, then in the bacterial strain of the mocha husband bacillus of 100 parts by weight inoculation of medium 2-5 parts by weight,
37 DEG C culture, until mocha husband bacillus viable count be 2-20 × 207/gram culture medium.
4. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 3, which is characterized in that the seed
The formula of culture medium is：Beef extract 3g, peptone 10g, sodium chloride 5g, agar 15-20g, distilled water 1000mL, pH value 7.0-
7.2,121 DEG C of sterilizing 30min；The formula of the 100 parts by weight culture medium is:Glucose 15g, starch 1g, beancake powder 25g, sulphur
Sour manganese 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, calcium carbonate 0.1g, pH7.0-7.2.
5. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 1, which is characterized in that the layer goes out
The cultural method of sickle-like bacteria is：It is 0.6-0.8 first fusarium prolifertum to be cultivated in PDA culture medium to strain density OD600 values, is obtained
To bacterium solution, then in the bacterial strain of the fusarium prolifertum of 100 parts by weight inoculation of medium 2-5 parts by weight, 37 DEG C of cultures, until layer
Go out sickle-like bacteria viable count be 2-20 × 207/gram culture medium.
6. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 5, which is characterized in that the PDA
The ingredient of culture medium is：Distilled water 1000mL, potato 200g, agar powder 20g, glucose 20g, 121 DEG C of air pressures 2.4 ×
Sterilize 20min under 105Pa；The formula of the 100 parts by weight culture medium is:Glucose 15g, starch 1g, beancake powder 25g, sulfuric acid
Manganese 1g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g, yeast extract 0.2g, iron chloride 0.1g, calcium carbonate 0.1g, pH7.0-7.2.
7. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 1, which is characterized in that the ginkgo
There is endogenetic fungus the cultural method of fusarium solani T-7 of broad spectrum antibiotic activity to be：The mycelia of inclined-plane culture is connected to PDA to consolidate
25-35 DEG C of body tablet is cultivated 72-120 hours, then is forwarded to 25-35 DEG C of PDA liquid medium and is activated 48-72 hours, by volume
Than 3-10% inoculum concentration, in liquid culture medium of transferring, pH to 6-9 at 25-35 DEG C, is cultivated 72 hours to 120 hours.
8. a kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt according to claim 7, which is characterized in that the PDA
The ingredient of solid and fluid nutrient medium is：Distilled water 1000mL, potato 200g, agar powder 20g, glucose 20g, 121 DEG C of gas
Sterilize 20min under 2.4 × 105Pa of pressure；The liquid culture medium is：Content 50% Gardenoside 20g/L, NaNO33g/L,
Remaining is water to KH2PO440g/L, with NaOH tune pH to 7.5.
9. a kind of preparation method of growth-promoting preparation described in claim 1, which is characterized in that include the following steps：(1) by mocha
It is 0.6-0.8 that husband bacillus, which is cultivated in seed culture medium to strain density OD600 values, bacterium solution is obtained, then in 100 parts by weight
The bacterial strain of the mocha husband bacillus of inoculation of medium 2-5 parts by weight, 37 DEG C of cultures, until the viable bacteria of mocha husband bacillus
Number be 2-20 × 207/gram culture medium；(2) fusarium prolifertum is cultivated to strain density OD600 values in PDA culture medium and is
0.6-0.8 obtains bacterium solution, then in the bacterial strain of the fusarium prolifertum of 100 parts by weight inoculation of medium 2-5 parts by weight, 37 DEG C
Culture, until fusarium prolifertum viable count be 2-20 × 207/gram culture medium；(3) inclined-plane culture is resisted with wide spectrum
The active gingko endogenous fungus fusarium solani T-7 of bacterium is connected to 25-35 DEG C of PDA solid plates and cultivates 72-120 hours, then transfers
It is activated 48-72 hours for 25-35 DEG C to PDA liquid medium, 3-10% inoculum concentrations, transfer into liquid culture medium by volume
In, pH to 6-9 at 25-35 DEG C, is cultivated 72 hours to 120 hours；(4) distilled water is added according to the ratio into container, at room temperature
Sealing a period of time；(5) at room temperature, mocha husband bacillus, fusarium prolifertum are then added according to the ratio successively and there is wide spectrum
The gingko endogenous fungus fusarium solani T-7 of antibacterial activity, concussion are uniform, you can.
10. a kind of application of growth-promoting preparation described in claim 1 in preventing ginger bacterial wilt.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439550A (en) * | 2018-11-20 | 2019-03-08 | 湖南科技学院 | The gingko endogenous ball Tuber Melanosporum of one plant of anti-ginger Ralstonia solanacearum and its application |
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