CN106342805A - Application of diisooctyl phthalate to control over root rot and bacterial wilt of hot pepper - Google Patents
Application of diisooctyl phthalate to control over root rot and bacterial wilt of hot pepper Download PDFInfo
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- CN106342805A CN106342805A CN201610774905.3A CN201610774905A CN106342805A CN 106342805 A CN106342805 A CN 106342805A CN 201610774905 A CN201610774905 A CN 201610774905A CN 106342805 A CN106342805 A CN 106342805A
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- diisooctyl phthalate
- hot pepper
- fusarium solani
- root rot
- bacterial wilt
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- Pest Control & Pesticides (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses application of diisooctyl phthalate to control over root rot and bacterial wilt of hot pepper. Experiments show that 0.125-0.2ml/L of diisooctyl phthalate compound preparation has the fusarium solani (Fusarium solani) inhibiting rate of 17-24.9%, so that the diisooctyl phthalate compound has certain hot pepper root rot-resisting fusarium solani activity. The diisooctyl phthalate compound is simple in structure, is easily artificially synthesized, is high in activity, has significant effects of controlling the fusarium solani-caused root rot and the pseudomonas (ralstonia solanacearum)-caused bacterial wilt of the hot pepper, does no harm to hot pepper plants, and can be applied to hot pepper production.
Description
Technical field
The present invention relates to phytochemistry and applied technical field, specially a kind of diisooctyl phthalate prevent and treat peppery
Application in pepper root maize ear rot.
Background technology
Fusarium solani (fusarium solani) be Fusarium solani pathogen, belong to Tuberculariaceae, Fusarium.
This pathogenic bacteria can reach more than 10 years by long-term surviving in soil, pathogenic bacteria with chlamydospore, mycelium or sclerotium with invalid body in soil
In survive the winter, the pathogenic bacteria that survives the winter at the beginning of next year propagates by rainwater etc., invades from rhizome portion, root wound, is produced by sick portion infecting
Conidium is by rainwater its spread in china.It is susceptible under the conditions of high-temperature high humidity climate, disease after the especially continuous rainfall a few days
Symptom showed increased.I.e. fall ill seriously in sequential cropping cultivation, the plot of impeded drainage.In current agricultural production, grafting preventing and treating is peppery
The application of pepper root maize ear rot is maximally efficient, but the higher complex operation of its cost of labor, therefore in the urgent need to developing effective preparation.
Pepper ralstonia solanacearum is by Pseudomonas alba(ralstonia solanacearum)The bacillary soil of one kind causing passes
Disease (yabuuchi etc., 1995), is common Fructus Capsici root disease, has destructive destructiveness.This pathogen is from host
The root of plant, is invaded xylem vessel, is expanded to rapidly the aerial partss of plant by fibrovascular system, pathogenic bacteria numerous in a large number
Grow and cause Catheter dysfunction, and then cause plant wilt, lead to Plant death (hayward, 1994).Pathogen is with invalid body
Soil is survived the winter, Second Year passes through the approach such as irrigation water, subterranean pest-insect, rainwater, operation instrument and propagates.Investigation shows, in canopy
It is the most important condition that pepper ralstonia solanacearum occurs that high temperature, high humidity and soil continuous cropping carry disease germs.Particularly suddenly fine after early summer heavy rain, potassium
Fertile not enough, again not in time, these situations are all beneficial to popular (Lu Jinhua, 1998) of disease for draining.In canopy the soil moisture reach 20 ~
25 DEG C, temperature is 30 ~ 35 DEG C, and when 95% ~ 100%, be that pepper ralstonia solanacearum is the most suitable occurs and spread relative air humidity.
Content of the invention
The present invention is directed to above weak point, there is provided a kind of diisooctyl phthalate application preventing and treating Fructus Capsici root-rot
Disease, tests proof 0.125 ~ 0.2mll-1Diisooctyl phthalate compound formulation to fusarium solani (fusarium solani) suppression ratio reach 17% ~ 24.9%, be that the preventing and treating of Fusarium solani provides a kind of new thinking.
The technical solution adopted for the present invention to solve the technical problems is: diisooctyl phthalate is in preventing and treating pepper root
Application in maize ear rot.
Further and, optium concentration when described diisooctyl phthalate uses is 0.125 ~ 0.2mll-1.
Described diisooctyl phthalate compound is to separate to obtain from Fructus Capsici root exudates, and its molecular formula is
c24h38o4, shown in its structural formula such as formula ():
, formula ().Plant diisooctyl phthalate compound of the present invention is first
It is separated the secondary disease-resistant Fructus Capsici stock from Zaozhuang agricultural science and technology Demonstration Garden and grafting chili pepper root exudates, pass through
Gas chromatography-mass spectrography (gc-ms) technology determines as diisooctyl phthalate compound, and characterizes it and specifically tie
Structure.Test proof 0.125 ~ 0.2mll-1Diisooctyl phthalate compound formulation to fusarium solani (fusarium solani) suppression ratio reach 17% ~ 24.9%, 29%-49% is reached to the bacteriostasis rate of pseudomonass, illustrates that compound has certain
Anti- Fusarium solani fusarium solani and the activity of Pseudomonas alba.The compounds of this invention structure is simple, and synthetic is easily real
Existing, the activity of compound is good, to the preventing and treating microbial Fusarium solani of fusarium solanae and Pseudomonas alba(ralstonia Solanacearum)The bacterial wilt causing has significant effect, and pepper plant is not injured, and can be used in the production of Fructus Capsici.
Brief description
It is the inhibitory action to Pathogens Causing Root Rot Disease for the variable concentrations diisooctyl phthalate shown in Fig. 1；
It is the inhibitory action to root rot and Stalk Rot for the variable concentrations diisooctyl phthalate shown in Fig. 2；
It is to mix Fusariumsp bacterium solution and Pseudomonas alba bacterium solution process Fructus Capsici respectively using diisooctyl phthalate shown in Fig. 3
After seedling, the state of Hot Pepper Seedling.
Specific embodiment
Describe the present invention with specific embodiment below in conjunction with the accompanying drawings:
First, the component analyses of Fructus Capsici root exudates
It it is resistance preferable Fructus Capsici stock variety ' bodyguard ' (ws, the day that this laboratory filters out in recent years for examination stock variety
This), scion is ' Xinfeng No.2 ' (xf) Fructus Capsici (thin skin type, Anhui Province Xiao County Xinfeng Fructus Capsici institute).Pathogens Causing Root Rot Disease is
Fusarium solani (fusarium solani) divide from the Fusarium solani disease plant of Zaozhuang, Shandong agricultural science and technology Demonstration Garden
From acquisition, Stalk Rot is Pseudomonas alba(ralstonia solanacearum)It is purchased from Guangzhou Microbiological Culture Collection
Center.Diisooctyl phthalate is purchased from Aladdin (aladdin) company.
Stock, grafting and the root exudates from Rhizoma schizomussaendae dehiscentis green pepper are analyzed, are listed in table 1.Result shows, from stock ws root
It is in secretions, to isolate 12 kinds of materials, main 7 kinds of hydro carbons of inclusion, 3 kinds of arene, a kind of esters, a kind of alcohols, a kind of esters
With a kind of heterocyclic compound.After grafting, hydro carbons, esters and alcohols material increase, and isolate 21 from grafting xf/ws root exudates
Plant material, wherein 12 kinds of hydro carbons, 3 kinds of arene, 2 kinds of esters, 2 kinds of alcohols, a kind of esters and a kind of heterocyclic compound.And from certainly
Only isolate 9 kinds of materials in Rhizoma schizomussaendae dehiscentis green pepper root exudates, have no alcohols, esters and heterocyclic compound.Wherein stock and grafting are peppery
In green pepper root exudates, material exclusive or that relative amount is higher is: 3- methyl -3- ethyl heptane, 5- butyl nonane, 14
Alkane, hexadecane, 2- methyl dodecane, 2,6,10,14,18- pentamethyl nonadecanes, o-Dimethylbenzene, diphenylene methane, 27
Alkanol, diethyl disulphide, diisooctyl phthalate and dibenzofurans are thus it is speculated that grafting chili pepper root exudates is to cause of disease
The inhibitory action of bacteria growing is relevant with above-mentioned substance.
The component analyses of table 1 Fructus Capsici root exudates
2nd, the biological function identification of allelochemical and checking
According to stock, grafting and the Fructus Capsici root exudates composition analysis result from Rhizoma schizomussaendae dehiscentis green pepper thus it is speculated that phthalic acid two is different pungent
Ester plays a significant role in strengthening grafting chili pepper disease resistance, therefore, the biological function of this material is identified and has verified.
1st, diisooctyl phthalate to root rot (f. solani) and ralstonia solanacearum (r. solanacearum)
Mlc screens
With diisooctyl phthalate hydroponics root rot and the Stalk Rot of variable concentrations, deionized water culture
For compareing, in 3 d post analysis root rot bacterium colony sizes and 24 h post analysis bacterial wilt colony counts.
The computational methods of pathogen bacteriostasis rate:
Funguses bacteriostasis rate (%)=(comparison ware colony diameter-process ware colony diameter)/comparison ware colony diameter × 100.
Antibacterial bacteriostasis rate (%)=(comparison ware colony counts-process ware colony counts)/comparison ware colony counts × 100.
Result shows, the bacteriostasis rate of diisooctyl phthalate solution is with 1.0 ml l-1Highest, funguses bacteriostasis rate is
35%, antibacterial bacteriostasis rate is 78.75%, 0.125,0.15,0.2 ml l-1With 0.5 ml l-1Secondly, funguses are antibacterial
Rate is respectively, and 17%, 18%, 25%, 26.5%, antibacterial bacteriostasis rate is respectively as follows: 29%, 33%, 49%, 65.5%, 0.05 ml l-1's
Fungistatic effect is poor, and 0.02 ml l-1No fungistatic effect (Fig. 1 and Fig. 2).
Preparing spore concentration with the diisooctyl phthalate solution of same concentrations is 5 × 106cfu· ml-1Sickle
Spore bacterium bacterium solution and concentration are about 3 × 108cfu · ml-1(a600=0.2) pseudomonass bacterium solution.With ' Xinfeng No.2 ' (xf) certainly
Root is examination material, according to the method inoculation of Wang Hui and Ren Xin just (1993), Investigate incidence after 5 d.Result shows, concentration is
0.125~1.0 ml · l-1The Fructus Capsici that processes of diisooctyl phthalate all do not fall ill, but concentration 0.5 ~ 1.0 ml
l-1Pepper Leaves have chlorosis phenomenon (Fig. 3).These results suggested that the suitable concentration of diisooctyl phthalate is 0.125%-0.2
ml · l-1.
2nd, the impact to Fructus Capsici soil-borne disease incidence and yield for the diisooctyl phthalate
After Fructus Capsici field planting, respectively with 0.125 ml l-1、0.15 ml · l-1、0.2 ml · l-1Phthalic acid two
Different monooctyl ester pouring root, 10 d fill 1 time, and 20 ml fill in period of duration 3 times altogether, to fill clear water for compareing (ck).Investigate before uprooting plants after their edible portions have been harvested
Each sickness rate processing.Result shows, the Fructus Capsici sickness rate that diisooctyl phthalate is processed is 0, and compares the root of Fructus Capsici
Maize ear rot sickness rate is 34.9%, has no bacterial wilt disease plant (table 2).
The impact to Fusarium solani and bacterial wilt sickness rate for table 2 diisooctyl phthalate
| Process | ck | 0.125 ml · l-1Adjacent benzene Dioctyl phthalate di-isooctyl | 0.15 ml · l-1Adjacent benzene Dioctyl phthalate di-isooctyl | 0.2 ml · l-1Adjacent benzene two Formic acid di-isooctyl |
| Root rot is sent out Sick rate (%) | 34.9± 0.15b | 0.00±0.00a | 0.00±0.00a | 0.00±0.00a |
| Bacterial wilt is sent out Sick rate (%) | 0.00± 0.00a | 0.00±0.00a | 0.00±0.00a | 0.00±0.00a |
As seen from Table 3, the Fructus Capsici single fruit weight with diisooctyl phthalate root irrigation is not different notable, but its individual plant fruit digital display
Write and be more than ck, therefore, 0.125 ml l-1、0.15 ml · l-1、0.2 ml · l-1Diisooctyl phthalate
Process yield higher by 19% than ck respectively, 25%, 31.7%.
The impact to yield of hot pepper for table 3 diisooctyl phthalate
Claims (4)
1. application on preventing and treating Fusarium solani for the diisooctyl phthalate.
2. application on preventing and treating Fusarium solani for the diisooctyl phthalate according to claim 1, its feature exists
In: optium concentration when described diisooctyl phthalate uses is 0.125 ~ 0.2ml l-1.
3. application on preventing and treating pepper ralstonia solanacearum for the diisooctyl phthalate.
4. application on preventing and treating pepper ralstonia solanacearum for the diisooctyl phthalate according to claim 3, its feature exists
In: optium concentration when described diisooctyl phthalate uses is 0.125 ~ 0.2ml l-1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624527A (en) * | 2018-05-12 | 2018-10-09 | 湖南科技学院 | A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt |
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| CN101427678A (en) * | 2008-07-05 | 2009-05-13 | 王金胜 | Plant source bactericide and artificial synthesis thereof |
| CN101889591A (en) * | 2010-07-19 | 2010-11-24 | 黑龙江大学 | Phellodendron essential oil for inhibiting plant pathogens and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP5112929B2 (en) * | 2008-03-28 | 2013-01-09 | 株式会社フジシールインターナショナル | Label body and container with label |
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| JPS5112929A (en) * | 1974-07-23 | 1976-01-31 | Hoechst Ag | SATSUKINSEISUIWAYOFUNMATSUNO SEIZOHOHO |
| CN101427678A (en) * | 2008-07-05 | 2009-05-13 | 王金胜 | Plant source bactericide and artificial synthesis thereof |
| CN101889591A (en) * | 2010-07-19 | 2010-11-24 | 黑龙江大学 | Phellodendron essential oil for inhibiting plant pathogens and preparation method thereof |
Non-Patent Citations (2)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108624527A (en) * | 2018-05-12 | 2018-10-09 | 湖南科技学院 | A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt |
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