CN106811434A - The production method of Nei Shengmohawei bacillus solid fungicides and its application - Google Patents
The production method of Nei Shengmohawei bacillus solid fungicides and its application Download PDFInfo
- Publication number
- CN106811434A CN106811434A CN201710206879.9A CN201710206879A CN106811434A CN 106811434 A CN106811434 A CN 106811434A CN 201710206879 A CN201710206879 A CN 201710206879A CN 106811434 A CN106811434 A CN 106811434A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- shengmohawei
- nei
- fermentation
- production method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
Production method and its application the invention discloses a kind of Nei Shengmohawei bacillus solid fungicide, belong to microorganism field, to solve the problems, such as that existing liquid fermentation bacterium solution presence storage is difficult, be difficult transport and secondary fermentation.Comprise the following steps：Fermented during Nei Shengmohawei bacillus (Bacillus mojavensis) bacterial strain is accessed into solid matrix, access amount is 4 12%, pH value is 7.0 8.0, fermentation time is 30 42h, fermentation temperature is 34 42 DEG C.The present invention is easy to store and is transported using the microbial bacterial agent of agricultural wastes solid fermentation, and easy to use, and advantage is obvious.The preventive effect of Nei Shengmohawei bacillus preventing and treating cucumber fusarium axysporum of the present invention and capsicum wilt is more than 61%; so as to overcome, the existing chemical pesticide feature of environmental protection is poor, be also easy to produce the resistance to the action of a drug and the defect of security difference; with the feature of environmental protection it is good, be not likely to produce the resistance to the action of a drug and security is good, growth-promoting (fertilizer) function; meet the national requirements of " subtracting medicine fat-reducing ", be also beneficial to environmental protection.
Description
Technical field
The invention belongs to microorganism field, and in particular to a kind of production method of Nei Shengmohawei bacillus solid fungicide
And its application.
Background technology
Into after 21 century, the problems such as based on chemical pesticide to pollution and the residues of pesticides of environment, with " public plant protection,
The proposition and practice of environment protective plant protecting " theory, biological pesticide focus of the research and development as China's biological industry, agri-scientific research and application,
Great research field and the direction of national medium ＆ long term sci-tech development program are listed in, environment protective plant protecting has become the weight of national development
Want demand and inexorable trend.Wherein obtain the concern of numerous scholars as the research of Antagonistic Fungi using endophytic bacterium especially.Plant
Endogenetic bacteria refers to live in plant, but does not have the bacterium of any substantial damage to plant, its pass through fixed nitrogen, Soluble phosphorus and
It is that it turns into biocontrol microorganisms to produce the biological functions such as IAA and improve anti-adversity ability, the particularly bacteria resistance functions such as drought resisting and the cold-resistant of host
The powerful support of resource.
Endophytic bacterium can also compete Vegetation space and induction plant acquisition by secreting antibacterial substance and pathogen
Disease resistance prevents or reduces the harm infecting or cause of the pathogen to plant.With micro- life that endogenetic bacteria is developed as microorganism resource
Thing agricultural chemicals is not likely to produce the resistance to the action of a drug, does not injure natural enemy, and can be using the production extensively such as agricultural byproducts, it is often more important that endogenetic bacteria
Can colonize in crop body, it might even be possible to propagated in the next generation by seed (nursery stock), worked for a long time, it is time saving and energy saving, it is high
Effect, is applied to after agricultural production be that production green or non-polluted farm product provide a new approach.Particularly biological pesticide
Utilize the fermenting and producings such as agricultural byproducts or agricultural wastes, on the one hand the biological pesticide of production is applied to agricultural, reduces chemistry more
The negative issue of agricultural chemicals, improves agricultural output and quality, on the other hand comprehensive utilization agricultural byproducts and agricultural wastes, improves
Its added value.
Microbial manure after being manured into soil, by the quick breeding of its specific bacterial strain, can be fixed as a kind of new-type fertilizer
Phosphorus, the potassium element of stationary state in nitrogen, release soil in air so that the nutrient potentiality of environment are given full play to, and are
A good edaphon environment is built in plant growth, is reducing fertilizer amount, is reducing environmental pollution, raising farming article
The aspects such as matter are significant.Especially integrate the composite microbial of antibacterial, fixed nitrogen, phosphorus decomposing, potassium decomposing and plant growth element
The research and development of thing fertilizer, play the role of very important in agricultural sustainable development.
The droop of the plants such as cucumber, watermelon, muskmelon and capsicum occurs generally, to give band in agricultural production big huge economy
Loss, and the sick pathogen can be with long-term surviving in soil, and general chemical pesticide receives soil physical chemistry after entering soil
Matter impact effect is poor, and the effect preferable agricultural chemicals longevity of residure is long, Practice for Pesticide Residue in Agricultural Products amount and quality safety is influenceed, in mesh
Preceding country proposes under the big policy of " subtract medicine fat-reducing " that the substitute for finding chemical pesticide is the task of top priority, and microbial pesticide and
Microbial manure is then one of option.
CN104152385A discloses one plant of Mo Hawei Bacillus strains, microbial bacterial agent and preparation method thereof and answers
With disclosing inhibiting rate and growth-promoting effect to potato of the Mo Hawei bacillus to potato gangrene germ etc., particularly
Report the liquid fermentation condition of Mo Hawei bacillus.But liquid fermentation bacterium solution is present, and storage is difficult, be difficult transport and secondary
The problems such as fermentation.
The content of the invention
It is existing to solve it is an object of the invention to provide a kind of production method of Nei Shengmohawei bacillus solid fungicide
Liquid fermentation bacterium solution has that storage is difficult, is difficult transport and secondary fermentation.
It is a further object to provide a kind of application of Nei Shengmohawei bacillus solid fungicide.
The present invention provide technical scheme be：A kind of production method of Nei Shengmohawei bacillus solid fungicide, including
Following steps：Fermented during Nei Shengmohawei bacillus (Bacillus mojavensis) bacterial strain is accessed into solid matrix, connect
Enter amount for 4-12%, pH value is 7.0-8.0, fermentation time is 30-42h, and fermentation temperature is 34-42 DEG C.
Preferably, the access amount of Nei Shengmohawei bacillus (Bacillus mojavensis) bacterial strain is 8%, pH value
It is 7.5, fermentation time is 36h, fermentation temperature is 38 DEG C.
Preferably, wheat bran accounts for 26.9%, corn stalk powder 20.9%, powdered rice hulls 20.9%, maize flour 17.9%, dregs of beans
13.4%th, glucose 20g/kg, urea 10g/kg, ferrous sulfate 15g/kg, material:Water=1:1.2.
Preferably, the viable count of Nei Shengmohawei bacillus (Bacillus mojavensis) thalline be 3.61 ×
1012CFU/mL-3.43×1013CFU/mL。
Preferably, the viable count of Nei Shengmohawei bacillus (Bacillus mojavensis) thalline be 3.43 ×
1013CFU/mL。
Application of the above-mentioned Nei Shengmohawei bacillus solid fungicide in preventing and treating cucumber fusarium axysporum and capsicum wilt.
Alpine Grasslands herbage endogenetic bacteria Mo Hawei bacillus of the present invention uses Mo Hawei disclosed in CN104152385A
Bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905.
The present invention is easy to store and is transported using the microbial bacterial agent of agricultural wastes solid fermentation, and easy to use, excellent
Point is obvious.
Nei Shengmohawei bacillus feature of the present invention is, on the one hand because Nei Shengmohawei bacillus can be colonized in plant
In object, it is affected by the external environment small, Biocontrol Effect stabilization is lasting, is propagated in the next generation even with approach such as seeds, another
The condition and the microbial pesticide of the solid fermentation production microbial pesticide of aspect Mo Hawei bacillus to cucumber fusarium axysporum and
Capsicum wilt prevention effect has no report.Nei Shengmohawei bacillus of the present invention prevents and treats cucumber fusarium axysporum and capsicum wilt
Preventive effect more than 61%, so as to overcome that the existing chemical pesticide feature of environmental protection is poor, be also easy to produce the resistance to the action of a drug and the defect of security difference, tool
Have that the feature of environmental protection is good, be not likely to produce the resistance to the action of a drug and security is good, growth-promoting (fertilizer) function, meet the national requirements of " subtracting medicine fat-reducing ",
It is also beneficial to environmental protection.
Specific embodiment
The following examples can further illustrate the present invention, but do not limit the invention in any way.
Embodiment 1：Microbial bacterial agent
Its active component is Mo Hawei bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905's
Thalline, wherein, containing Mo Hawei bacillus 4% by weight percentage, matrix surplus, matrix is by following material composition：
Wheat bran accounts for 26.9%, corn stalk powder 20.9%, powdered rice hulls 20.9%, maize flour 17.9%, dregs of beans 13.4%, glucose 20g/
Kg, urea 10g/kg, ferrous sulfate 15g/kg.The viable count of Mo Hawei bacillus is 3.61 × 1012CFU/mL。
Embodiment 2：The preparation method of microbial bacterial agent, ferments during Mo Hawei bacillus is accessed into matrix, material-water ratio
It is 1：1.2, access amount is 4%, pH7.0, and fermentation time is 30h, and fermentation temperature is 34 DEG C, prepared microbial bacterial agent used as pesticides
Or fertilizer.
Embodiment 3：Microbial bacterial agent, its active component is Mo Hawei bacillus (Bacillus mojavensis)
The thalline of QLY002, CGMCC No.8905, wherein, containing Mo Hawei bacillus 8% by weight percentage, more than matrix
Amount, matrix is by following material composition：Wheat bran accounts for 26.9%, corn stalk powder 20.9%, powdered rice hulls 20.9%, maize flour
17.9%th, dregs of beans 13.4%, glucose 20g/kg, urea 10g/kg, ferrous sulfate 15g/kg.The viable count of Mo Hawei bacillus
It is 3.43 × 1013CFU/mL。
Embodiment 4：The preparation method of microbial bacterial agent, ferments during Mo Hawei bacillus thalline are accessed into matrix, connects
Enter amount for 8%, pH7.5, fermentation time is 36h, fermentation temperature is 38 DEG C, and microbial bacterial agent used as pesticides or fertilizer is obtained.
Embodiment 5：Microbial bacterial agent, its active component is above-mentioned Mo Hawei bacillus (Bacillus
The thalline of mojavensis) QLY002, CGMCC No.8905, wherein, contain Mo Hawei bacillus by weight percentage
12%, matrix surplus, matrix is by following material composition：Wheat bran account for 26.9%, corn stalk powder 20.9%, powdered rice hulls 20.9%,
Maize flour 17.9%, dregs of beans 13.4%, glucose 20g/kg, urea 10g/kg, ferrous sulfate 15g/kg.Mo Hawei bacillus
Viable count is 1.0 × 1013CFU/mL。
Embodiment 6：The preparation method of microbial bacterial agent, by Mo Hawei bacillus (Bacillus mojavensis)
QLY002, CGMCC No.8905 thalline ferment in accessing to matrix, and access amount is 12%, and fermentation time is 42h, fermentation temperature
It it is 42 DEG C, prepared microbial bacterial agent can use used as pesticides or fertilizer.
Below by two couples of Mo Hawei bacillus (Bacillus mojavensis) QLY002 of experiment one and experiment,
The solid fermentation matrix of CGMCC No.8905 bacterial strains is optimized.
Experiment one：Mo Hawei bacillus mojavensis' QLY002 (CGMCC No.8905) is most suitable
Solid fermentation matrix：
1st, single-matrix screening
It is single with 9 kinds of wheat bran, corn stalk powder, cornstarch, maize flour, bean powder, dregs of beans, the dregs of fat, rice bran and iblet
Matrix is the exclusive source of nutrition, by solid fermentation 36 hours, Mo Hawei bacillus (Bacillus during with dregs of beans as matrix
Mojavensis) viable count of QLY002 is up to 2.85 × 109Cfu/g, the viable count with rice bran and iblet as matrix is most
It is low, it is respectively 0.47 × 109Cfu/g and 0.48 × 109Cfu/g, be below in GB to microorganism medicine fertilizer viable count (≤0.5 ×
109Cfu/g requirement), during with wheat bran, corn stalk powder, cornstarch, maize flour, bean powder, dregs of beans and the dregs of fat as sole substrate
Viable count is higher than GB, therefore selection wheat bran, corn stalk powder, cornstarch, maize flour, bean powder, dregs of beans, the dregs of fat are Mo Hawei
The single-matrix of bacillus (Bacillus mojavensis) QLY002 medicines fertilizer solid fermentation.
2nd, composite interstitial substance component screening
In the single-matrix that obtains of screening, cornstarch and maize flour are mainly thalli growth and provide carbon source, bean powder and
Dregs of beans mainly provides nitrogen source, and wheat bran and corn stalk powder are not only thalli growth offer carbon source, but also is thalli growth
Carrier, therefore be mutually combined matrix with carbon nitrogen source by fermentation carrier in this experimental design, as following 12 combinations：
(1) wheat bran+powdered rice hulls+cornstarch+bean powder, (2) wheat bran+powdered rice hulls+cornstarch+dregs of beans, (3) wheat bran+powdered rice hulls+corn
Face+bean powder, (4) wheat bran+powdered rice hulls+maize flour+dregs of beans, (5) corn stalk powder+powdered rice hulls+cornstarch+bean powder, (6) corn
Powder of straw+powdered rice hulls+cornstarch+dregs of beans, (7) corn stalk powder+powdered rice hulls+maize flour+bean powder, (8) corn stalk powder+rice
Shell powder+maize flour+dregs of beans, (9) wheat bran+corn stalk powder+powdered rice hulls+cornstarch+bean powder (10) wheat bran+corn stalk powder+
Powdered rice hulls+cornstarch+dregs of beans, (11) wheat bran+corn stalk powder+powdered rice hulls+maize flour+bean powder and (12) wheat bran+corn stalk
Stalk powder+powdered rice hulls+maize flour+dregs of beans, through solid fermentation 36 hours, combines 12, i.e. wheat bran+corn stalk powder+powdered rice hulls powder+jade
The viable count highest of rice and flour+bean pulp fermentation product, reaches 7.05 × 109Cfu/g, is significantly higher than other combinations, i.e., this is combined as
Optimum combination.
3rd, compound substrate formula orthogonal test
Orthogonal test combination is shown in Table 1.Mo Hawei bacillus (Bacillus can be drawn by Tables 1 and 2
Mojavensis) bacterial strain QLY002 solid fermentations 36 hours, are processing 8 (A1B3C3D4E3) in viable count highest, reach 5.57
×1010Cfu/g, now fermentation substrate content is wheat bran 300g, corn stalk powder 233g, powdered rice hulls 233g, maize flour 200g, beans
Dregs of rice 150g, is converted into volume i.e. wheat bran and accounts for 26.9%, corn stalk powder and account for 20.9%, powdered rice hulls and accounted for 20.9%, maize flour
Account for 17.9%, dregs of beans and account for 13.4%.
Orthogonal test (the L of table 125(45)) interpretation of result
4th, carbon source optimizing
In optimum substrate culture medium glucose, sucrose, maltose, brown sugar, 5 kinds of white granulated sugar are added by addition 15g/kg not
Same carbon source through fermentation 36 hours, during with glucose as carbon source, viable count is significantly higher than other carbon sources, reaches 7.68 × 1011cfu/
G, i.e. glucose are the optimal carbon that Mo Hawei bacillus (Bacillus mojavensis) bacterial strain QLY002 carries out solid fermentation
Source.
5th, nitrogen source optimization
Beef extract, peptone, yeast extract, 5 kinds of different nitrogen sources of urea and ammonium nitrate are added in optimum substrate culture medium
10g/kg carries out fermentation 36 hours, and result of the test shows, during with urea as nitrogen source, viable count is 7.15 × 1011Cfu/g, bacterial strain life
Amount long illustrates that Mo Hawei bacillus (Bacillus mojavensis) QLY002 grows apparently higher than other nitrogen source mediums
Most suitable nitrogen source be urea.
6th, inorganic salts optimization
10g/kg additions sodium chloride, magnesium sulfate, manganese sulfate, ferrous sulfate, biphosphate are pressed in optimum substrate culture medium
5 kinds of inorganic salts of potassium ferment 36 hours, and result of the test shows, the viable count after being fermented 36 hours as inorganic salts with ferrous sulfate is notable
Higher than other inorganic salts, up to 7.43 × 1011cfu/g.Illustrate Mo Hawei bacillus (Bacillus mojavensis) QLY002
The most suitable inorganic salts of growth are ferrous sulfate.
7th, most suitable C sources, N sources, the determination of inorganic salts best in quality proportioning
It is orthogonal using table 2 to most suitable C sources (glucose), most suitable N sources (urea) and most suitable 3 factors of inorganic salts (ferrous sulfate)
Design table L9(33) orthogonal test is carried out, when being combined as G3U1F3When, i.e. glucose 20g/kg, urea 10g/kg and ferrous sulfate 15g/
Kg, 36 hours viable counts of solid fermentation are significantly higher than other combinations, reach 3.55 × 1012Cfu/g, i.e., it is most suitable C sources, N sources, inorganic
Salt best in quality proportioning is respectively glucose 20g/kg, urea 10g/kg and ferrous sulfate 15g/kg.
Orthogonal test (the L of table 216(43)) interpretation of result
Experiment two：Determine Mo Hawei bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905's
Solid fermentation condition：
1. inoculum concentration, water content and temperature orthogonal test
Based on testing an optimum optimization culture medium for obtaining, inoculum concentration, water content, the temperature that solid fermentation is related to
The conditions such as degree are done the orthogonal test (table 3) of the level of three factor five by different levels respectively, each 4 repetition for the treatment of.Experiment knot
Fruit shows that solid fermentation condition is combined as A3B4C4D3When, viable count is significantly higher than other combinations, reaches 2.84 × 1013cfu/
G, i.e. optimum condition are inoculum concentration 8%, water content 1:1.2nd, 38 DEG C of temperature.
Orthogonal test (the L of table 325(54)) factor level table
2. optimal initial pH value optimization
The initial pH value of fermentation medium is adjusted to 6.0,6.5,7.0,7.5,8.0 this 5 levels with phosphate buffer,
Fermented by different initial pH values on the basis of two first steps of experiment one and experiment, each 4 repetition for the treatment of.Result of the test table
Bright, the viable count highest when initial pH value is 7.5, is 3.4 × 1013Cfu/g, and pH be 7.0,8.0 when, viable count difference does not show
Viable count when writing, but being below 7.5, viable count is substantially reduced when pH is 6.0,6.5.Accordingly, it is determined that the optimum pH of the bacterium
It is 7.5.
3. the determination of optimal fermentation time
On the basis of 2 the first/bis- steps of experiment one and experiment, take appropriate fermentate every 6h and determine viable count.Experiment knot
Fruit shows that during from 6h to 36h, viable count is dramatically increased, and as fermentation 36h, viable count is 3.43 × 10 to the maximum13Cfu/g, and from
Viable count is significantly reduced after 36h.Therefore most suitable fermentation time final choice 36h.
Experiment three：To Mo Hawei bacillus (Bacillus mojavensis) the micro- life of QLY002, CGMCC No.8905
The activity of thing microbial inoculum is detected：
The most suitable solid matrix and fermentation condition of Mo Hawei bacillus QLY002 are had determined that in above-mentioned experiment one and two
On the basis of, the Mo Hawei bacillus mojavensis QLY002 (CGMCC in slant medium will be stored in
No.8905), after cultivating activation 24h on the plate of nutrient agar (Nutrient Agar, abbreviation NA) culture medium, it is inoculated in battalion
In foster meat soup (Nutrient Broth, abbreviation NB) culture medium；After culture 24h, solid is accessed with the inoculum concentration of 4%-12%
In matrix, fermentation material-water ratio is 1:After 1.2,34 DEG C of -42 DEG C of fermented and cultured 30h-42h, in fermentate viable count be 3.61 ×
1012CFU/mL-3.43×1013CFU/mL。
By Mo Hawei bacillus QLY002 under above-mentioned fermentation condition solid fermentation, you can be obtained with good growth-promoting
The microbial bacterial agent of ability and diseases prevention ability.Wherein, it is 38 DEG C in temperature, inoculum concentration is 8%, fermentation material-water ratio is 1:1.2, hair
Under the ferment time is for the fermentation condition of 36h, viable count can reach 3.43 × 10 in fermentate13CFU/mL。
Below by experiment four and experiment five respectively to Mo Hawei bacillus mojavensis QLY002
(CGMCC No.8905) microbial inoculum is to the growth-promoting ability of cucumber and capsicum and prevents and treats droop ability and detects.
Experiment four：Mo Hawei bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905 solid bacteriums
Agent is to cucumber and the growth-promoting effect measuring of capsicum：
After by 121 DEG C of autoclaving 2h of Nutrition Soil used, Mo Hawei bacillus QLY002 medicines fertilizer is added in mass ratio, it is dense
Degree is respectively 0%, 0.5%, 1%, 5%, 10%, is sub-packed in after stirring and evenly mixing in the plastic flowerpot of a diameter of 12cm, wherein 0%
It is control, cucumber and pepper seed is sowed for 10 by every basin.12d counts the emergence rate of each plant, each treatment 4 times
Repeat.Result shows that 1% medicine fertilizer is emerged to cucumber and pepper seed and has facilitation (table 4)；It is right after the fertilizer treatment of various concentrations medicine
The cucumber of 40d seedling ages, determines plant height, root is long, stem is thick, fresh weight and dry weight, and capsicum is 55d seedling ages, measure plant height, root is long, stem is thick,
Fresh weight and dry weight.Result shows, 1% medicine fertilizer growth-promoting effect is significant (table 5 and table 6).
Influence (%) of the different medicine fertilizer concentration of table 4 to emergence rate
Influence (%) of the different medicine fertilizer concentration of table 5 to cucumber growth index
| Treatment | Plant height/cm | Root is long/cm | Stem is thick/cm | Overground part fresh weight/g | Underground part fresh weight/g | Overground part dry weight/g | Underground part dry weight/g |
| % | 44.66±0.71b | 24.20±0.65c | 0.326±0.014d | 6.36±0.03c | 0.84±0.04c | 0.42±0.01c | 0.09±0.01bc |
| 0.5% | 46.38±0.81b | 26.62±0.91b | 0.420±0.009b | 7.94±0.12b | 1.18±0.02b | 0.70±0.05b | 0.10±0.01b |
| 1.0% | 54.04±0.59a | 30.10±0.87a | 0.522±0.012a | 11.17±0.08a | 1.47±0.05a | 1.17±0.03a | 0.14±0.01a |
| 5.0% | 40.78±0.52c | 22.02±0.82c | 0.374±0.007c | 5.32±0.09d | 0.39±0.02d | 0.35±0.02c | 0.06±0.01cd |
| 10.0% | 37.28±0.72d | 18.54±0.48d | 0.320±0.016d | 4.39±0.12e | 0.36±0.03d | 0.34±0.02c | 0.05±0.01d |
Influence (%) of the different medicine fertilizer concentration of table 6 to chili growth index
| Treatment | Plant height/cm | Root is long/cm | Stem is thick/cm | Overground part fresh weight/g | Underground part fresh weight/g | Overground part dry weight/g | Underground part dry weight/g |
| 0% | 40.44±1.01bc | 12.46±0.49b | 0.338±0.012c | 3.91±0.02b | 0.73±0.02d | 0.30±0.01b | 0.08±0.00b |
| 0.5% | 41.90±0.68b | 13.54±0.36b | 0.378±0.013b | 3.95±0.01b | 0.92±0.01b | 0.32±0.01b | 0.09±0.01b |
| 1.0% | 47.32±1.29a | 14.94±0.35a | 0.452±0.011a | 8.06±0.03a | 1.79±0.02a | 0.72±0.01a | 0.18±0.01a |
| 5.0% | 38.44±1.09c | 13.10±0.35b | 0.320±0.005c | 3.03±0.01c | 0.79±0.01c | 0.21±0.01c | 0.08±0.01b |
| 10.0% | 33.56±0.72d | 10.24±0.65c | 0.250±0.006d | 2.43±0.01d | 0.42±0.01e | 0.19±0.01c | 0.05±0.00c |
Experiment five：Mo Hawei bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905 solid bacteriums
Agent is determined to the prevention effect of cucumber and capsicum wilt：
After by 121 DEG C of autoclaving 2h of Nutrition Soil used, by cucumber and pepper planting in the plastic flowerpot of diameter 12cm,
After emerging, per 6 plants of basin final singling, 4 repetitions.When cucumber is long to 30d seedling ages, during capsicum 45d seedling ages, with the optimal medicine fertilizer for screening
Concentration is that consumption sets following treatment：
1 (FB) for the treatment of：The soil on flowerpot surface is deducted with sterile scalpel, medicine fertilizer is applied by optimal medicine fertilizer concentration, after 7d
Wound is manufactured in plant basal part of stem, it is 10 that sickle-like bacteria Fusarium oxysporum sterilized waters are configured into concentration6~107Individual/
The spore suspension of mL, wound location, room temperature moisturizing training are inoculated in by the spore suspension with the aseptic injector for medical purpose of 10mL
Support 48h；
2 (B) for the treatment of：Inoculation method first connects sickle-like bacteria Fusarium oxysporum spore suspensions, after 7d with treatment 1
Apply medicine fertilizer.
3 (BF) for the treatment of：Only inoculation sickle-like bacteria Fusarium oxysporum spore suspensions, medicine fertilizer, inoculation side are not applied
Method is with treatment 1.
Cucumber fusarium axysporum disease scale standard
0 is asymptomatic, plant normal growth；
The slight yellowing of 1 cauline leaf；
2 yellowing leafs, plant is slightly wilted, and can be recovered；
3 yellowing leafs are sagging, and plant moderate is wilted, irrecoverable；
4 plant are seriously wilted, withered.
Capsicum wilt disease scale standard
0 is asymptomatic, plant normal growth；
1 plant vascular bundle slightly becomes brown；
2 plant vascular bundle brownings, and area accounts for the 10%~30% of whole vascular bundle tangent plane；
3 plant vascular bundles seriously rot, and area accounts for the gross area 30%~50%；
The rotten area of 4 plant vascular bundles accounts for more than the 50% of the gross area.
By cucumber and the droop disease scale standard survey incidence of disease and disease index of capsicum after 15d, Mo Hawei is calculated
Preventive effect of bacillus mojavensis QLY002 (CGMCC No.8905) medicine fertilizer to droop.Result shows,
Treatment FB is compared with treatment B, and the incidence of disease of cucumber and capsicum reduces 37.50% and 45.83% respectively, and disease index drops respectively
Low by 34.24 and 33.3, prevention effect is respectively 61.83% and 63.96% (table 7 and table 8), but BF to the prevention effect of B only
Respectively 36.59% and 24.63%.Therefore, Mo Hawei bacillus mojavensis QLY002 (CGMCC
No.8905) medicine fertilizer has good preventive effect to droop.
The cucumber fusarium axysporum incidence of table 7
| Project | The incidence of disease (%) | Disease index | Prevention effect (%) |
| 1 (FB) for the treatment of | 54.17c | 19.80c | 61.83 |
| 2 (BF) for the treatment of | 75.00b | 32.29b | 36.59 |
| 3 (B) for the treatment of | 91.67a | 54.04a | - |
The capsicum wilt incidence of table 8
| Project | The incidence of disease (%) | Disease index | Prevention effect (%) |
| 1 (FB) for the treatment of | 50.00c | 17.71c | 63.96 |
| 2 (BF) for the treatment of | 79.41b | 38.54b | 24.63 |
| 3 (B) for the treatment of | 95.83a | 51.04a | - |
Experiment proves that, above-mentioned Mo Hawei bacillus (Bacillus mojavensis) QLY002, CGMCC
No.8905 microbial bacterial agents, are suitable for the preventing and treating of cucumber fusarium axysporum and capsicum wilt.
Claims (6)
1. a kind of production method of Nei Shengmohawei bacillus solid fungicide, it is characterised in that comprise the following steps：By interior life
Mo Hawei bacillus (Bacillus mojavensis) bacterial strain ferments in accessing to solid matrix, and access amount is 4-12%, pH
It is 7.0-8.0 to be worth, and fermentation time is 30-42h, and fermentation temperature is 34-42 DEG C.
2. the production method of Nei Shengmohawei bacillus solid fungicide according to claim 1, it is characterised in that：Interior life
The access amount of Mo Hawei bacillus (Bacillus mojavensis) bacterial strain is 8%, and pH value is 7.5, and fermentation time is 36h,
Fermentation temperature is 38 DEG C.
3. the production method of Nei Shengmohawei bacillus solid fungicide according to claim 1 and 2, it is characterised in that：
The solid matrix is by following material composition：Wheat bran accounts for 26.9%, corn stalk powder 20.9%, powdered rice hulls 20.9%, maize flour
17.9%th, dregs of beans 13.4%, glucose 20g/kg, urea 10g/kg, ferrous sulfate 15g/kg, material:Water=1:1.2.
4. the production method of Nei Shengmohawei bacillus solid fungicide according to claim 1, it is characterised in that：Interior life
The viable count of Mo Hawei bacillus (Bacillus mojavensis) thalline is 3.61 × 1012CFU/mL-3.43×
1013CFU/mL。
5. the production method of Nei Shengmohawei bacillus solid fungicide according to claim 4, it is characterised in that：Interior life
The viable count of Mo Hawei bacillus (Bacillus mojavensis) thalline is 3.43 × 1013CFU/mL。
6. Nei Shengmohawei bacillus solid fungicide described in claim 1 is in preventing and treating cucumber fusarium axysporum and capsicum wilt
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710206879.9A CN106811434A (en) | 2017-03-31 | 2017-03-31 | The production method of Nei Shengmohawei bacillus solid fungicides and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710206879.9A CN106811434A (en) | 2017-03-31 | 2017-03-31 | The production method of Nei Shengmohawei bacillus solid fungicides and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106811434A true CN106811434A (en) | 2017-06-09 |
Family
ID=59115996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710206879.9A Pending CN106811434A (en) | 2017-03-31 | 2017-03-31 | The production method of Nei Shengmohawei bacillus solid fungicides and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106811434A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624527A (en) * | 2018-05-12 | 2018-10-09 | 湖南科技学院 | A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt |
| CN112322562A (en) * | 2020-12-25 | 2021-02-05 | 广西壮族自治区畜牧研究所 | Solid culture medium of bacillus, preparation method thereof and bacillus culture method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152385A (en) * | 2014-08-11 | 2014-11-19 | 甘肃农业大学 | Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum |
| US20150147296A1 (en) * | 2011-09-08 | 2015-05-28 | László Manczinger | Copper resistant, fengycin-producing bacillus mojavensis strain for controlling vegetable pathogens, its use and compositions containing it |
| CN105624061A (en) * | 2016-01-08 | 2016-06-01 | 陕西博秦生物工程有限公司 | Bacillus amyloliquefaciens subsp.amyloliquefaciens as well as preparation and application of solid bacterial preparation of bacillus amyloliquefaciens subsp.amyloliquefaciens |
| CN106350470A (en) * | 2016-11-18 | 2017-01-25 | 甘肃农业大学 | Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum |
-
2017
- 2017-03-31 CN CN201710206879.9A patent/CN106811434A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150147296A1 (en) * | 2011-09-08 | 2015-05-28 | László Manczinger | Copper resistant, fengycin-producing bacillus mojavensis strain for controlling vegetable pathogens, its use and compositions containing it |
| CN104152385A (en) * | 2014-08-11 | 2014-11-19 | 甘肃农业大学 | Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum |
| CN105624061A (en) * | 2016-01-08 | 2016-06-01 | 陕西博秦生物工程有限公司 | Bacillus amyloliquefaciens subsp.amyloliquefaciens as well as preparation and application of solid bacterial preparation of bacillus amyloliquefaciens subsp.amyloliquefaciens |
| CN106350470A (en) * | 2016-11-18 | 2017-01-25 | 甘肃农业大学 | Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum |
Non-Patent Citations (4)
| Title |
|---|
| 刁治民等: "《农业微生物工程学》", 31 December 2007, 青海人民出版社 * |
| 孙倩等: "地衣芽孢杆菌固体发酵培养基优化研究", 《食品工业》 * |
| 徐雪莲等: "2株抗枯萎病尖镰孢菌内生细菌菌株的分离及鉴定", 《果树学报》 * |
| 林天兴等: "高产铁载体棉田土壤细菌SS05的筛选与鉴定", 《微生物学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624527A (en) * | 2018-05-12 | 2018-10-09 | 湖南科技学院 | A kind of ginkgo source growth-promoting preparation of prevention ginger bacterial wilt |
| CN112322562A (en) * | 2020-12-25 | 2021-02-05 | 广西壮族自治区畜牧研究所 | Solid culture medium of bacillus, preparation method thereof and bacillus culture method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106350470B (en) | The production method and its application of bacillus amyloliquefaciens and microbial inoculum and the microbial inoculum | |
| CN104531533B (en) | A kind of composite bacteria agent for preventing and treating plant continuous cropping disease and preparation method thereof, application | |
| CN101696390B (en) | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof | |
| CN101659933B (en) | Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof | |
| CN102816725B (en) | Bacillus subtilis and application thereof | |
| CN104195074B (en) | A kind of deep brown Bacillus strain of Alpine Grasslands herbage endogenetic bacteria, microbial bacterial agent and its preparation method and application | |
| CN104962490B (en) | A kind of Enteromorpha microbial bacterial agent and preparation method thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN104447001A (en) | Special compound microbial fertilizer for rice | |
| CN101696395B (en) | Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof | |
| CN103146586A (en) | Trichoderma harzianum strain for controlling plant fungus diseases and application thereof | |
| CN105838656A (en) | Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus | |
| CN107502572A (en) | Application of the bacillus licheniformis in straw degradative, the microbial bacterial agent comprising the bacterium and its application | |
| CN105439723A (en) | Bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN110577916A (en) | Compound microbial inoculum for preventing and treating crop root-knot nematode and preparation method thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN105481489A (en) | Bacillus subtillis biological bacterial fertilizer and preparation method thereof | |
| CN107711891A (en) | A kind of method prevented and treated matrimony vine root rot and extremely set | |
| CN105567568A (en) | Microbial agent for preventing and controlling solanaceous vegetable virosis and preparation method thereof | |
| CN106434458A (en) | Paenibacillus polymyxa, inoculant thereof, preparation of inoculant, and applications of paenibacillus polymyxa and inoculant | |
| CN112746024A (en) | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same | |
| CN107384825A (en) | Agricultural microorganism preparation and its application in terms of vega continuous cropping obstacle is overcome | |
| CN108395893A (en) | A kind of improvement soil complex micro organism fungicide and preparation method thereof | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN109315265B (en) | Preparation method and application of chaetomium globosum biological seedling culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170609 |