CN110250216A - The new application of Herba laggera volatile oil - Google Patents

The new application of Herba laggera volatile oil Download PDF

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Publication number
CN110250216A
CN110250216A CN201910715508.2A CN201910715508A CN110250216A CN 110250216 A CN110250216 A CN 110250216A CN 201910715508 A CN201910715508 A CN 201910715508A CN 110250216 A CN110250216 A CN 110250216A
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volatile oil
herba laggera
pathogenic bacteria
laggera
herba
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董鲜
程永现
曾子盈
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Shenzhen University
Yunnan University of Traditional Chinese Medicine TCM
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Shenzhen University
Yunnan University of Traditional Chinese Medicine TCM
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of Herba laggera volatile oils in new application agriculturally；That is its application in prevention and treatment Chinese medicine or crop root rot or the application in the pathogenic bacteria that prevention and treatment causes Chinese medicine or crop overground part that disease occurs；The results showed that Herba laggera volatile oil is to causing, 4 kinds of main pathogenic bacteria Fusarium oxysporums of notoginseng root rot generation, Fusarium solani, rotten mould and breaking-up column spore is mould and the mycelia growth of 3 kinds of pathogenic bacteria botrytis cinereas of Radix Notoginseng overground part disease generation, colletotrichum gloeosporioides Penz, Rhizoctonia solani Kuhn is caused to have inhibiting effect, when concentration is 50mg/mL, for causing the pathogenic bacteria mycelia growth inhibitory effect of Radix Notoginseng aerial part disease stronger；Pass through the analysis to FIC value, it was found that Herba laggera volatile oil and hymexazol combination have additive effect and synergistic effect to a variety of pathogens, volatile oil is volatile, and has many advantages, such as tourette production of aromatic, thus with its exploitation chemical fertilizer or pesticide will have safely, effectively, low-residual.

Description

The new application of Herba laggera volatile oil
Technical field
The present invention relates to the purposes of Herba laggera volatile oil more particularly to Herba laggera volatile oil to Chinese medicine or crop root rot And the preventive and therapeutic effect to Chinese medicine or crop overground part multiple diseases, belong to biological pesticide technical field.
Background technique
Bacteriostatic agent is exactly the substance that pathogenic bacteria can be inhibited to grow；Bacteriostatic agent possibly can not kill pathogenic bacteria, but it can press down The growth of pathogenic bacteria processed prevents pathogenic bacteria from breeding excessive.Plant volatile oil is used for traditional medicine, food, tobacco, alcohol and cigarette Grass is also used to plant protection (desinsection, antibacterial, weeding etc.), this without the utilization suspected of plant ecosystem open one it is new Approach；Plant volatile oil is applied to plant protection art as botanical pesticide, consistent with the public nuisance-free agricultural chemicals theory of suggestion；From It is said in this meaning, plant volatile oil can also be classified as third generation pesticide；Some essential oils only inhibit certain special germs Growth, and some essential oils, inhibit the type of germ very much.
Radix Notoginseng is continuously increased as rare cultivation medicinal plant social required quantity, with the rapid increasing of notoginseng planting area Add, disease problem is got worse, it has also become the major obstacle of Radix Notoginseng production；Currently, being relied primarily on to the prevention and treatment of Radix Notoginseng disease Various chemical agents, though chemical pesticide effectively, considerably increases the input cost of peasant, more seriously polluted soil and reduction Medical material quanlity；There are problems that " 3R " (residual, drug resistance and regeneration), the agriculture of high residue when using chemical bactericide, insecticide Medicine will cause serious environmental pollution.
Laggera pterodonta (Laggera pterodonta) also known as deer ear woods or wingedstem inula root, composite family Laggera plant, with root or All herbal medicine；It is clearing heat and detoxicating, control the infection of the upper respiratory tract, tonsillitis, sphagitis, stomatitis, bronchitis, malaria, carbuncle swells sore Furuncle etc..
Currently, having no that document relevant to technical solution of the present invention discloses.
Summary of the invention
It is an object of that present invention to provide a kind of new applications of Herba laggera volatile oil, i.e., it is in prevention and treatment Chinese medicine or crop root-rot Application in disease, the pathogenic bacteria of the root rot are Fusarium oxysporum (Fusarium oxysporum), Fusarium solani (Fusarium solani), rotten mould (Pythium aphanidermatum), the mould (Cylindrocarpon of column spore is damaged Destructans) etc..
Herba laggera volatile oil of the present invention, which also can be applicable to prevention and treatment, causes Chinese medicine or crop overground part that causing a disease for disease occurs In bacterium；The pathogenic bacteria are botrytis cinerea (Botrytis cinerea), colletotrichum gloeosporioides Penz (Colletotrichum Gloeosporioides), Rhizoctonia solani Kuhn (Rhizoctonia solani) etc..
The purposes of Herba laggera volatile oil of the present invention is that Herba laggera volatile oil is used as to preparation prevention and treatment Chinese medicine or work Object root rot or prevention and treatment cause the preparation of the pathogenic bacteria of Chinese medicine or crop overground part generation disease, i.e., with Herba laggera volatile oil For active constituent, the application in the bacteria preparation that causes a disease is prevented and treated in preparation.
The ingredient (or effective component) of the present invention for preventing and treating the bacteria preparation that causes a disease is Herba laggera volatile oil, can also be added one Acceptable auxiliary material in kind or plurality of Chinese and control of crop disease preparation, or played with other active components compounding and cooperate with suppression Bacterium effect.
Herba laggera volatile oil can be prepared into the pesticide of various dosage forms or the chemical fertilizer of various forms by prior art device.
Herba laggera volatile oil is extracted by the conventional steam way of distillation in the present invention, anhydrous sodium sulfate drying and volatilizing oil and It is made, the preparation method is simple, and required reagent cost is low, is conducive to mass production.
Herba laggera volatile oil is as a kind of natural Fungicidal active substance, its activity disappears or by microorganism point after use Solution, no pollution to the environment, to safety of human and livestock；Since Herba laggera volatile oil is Extracts from Plant Recourses, effective component is complicated various, Be the mechanism of action of multicomponent multiple target point to pathogen so that target organisms be not likely to produce several ingredients of certain or certain are generated it is anti- Pharmacological property, to obtain lasting control efficiency.Compared with chemical synthetic pesticide, the effective component of Herba laggera volatile oil is volatile, It is degradable, it is antibacterial without sterilizing, therefore be not easy to cause damages to other beneficial microbes, improve soil micro-ecosystem structure, promotees It into plant and microorganism coevolution, preserves the ecological environment, improves the quality of Chinese medicine or crop.
Herba laggera volatile oil can reduce plant disease incidence and the state of an illness by inhibiting pathogen spore germination and mycelia growth Index；By with chemical pesticide synergy, achieve the purpose that reduce chemical pesticide application.
The Herba laggera volatile oil solution of 50mg/mL is prepared, solvent is DMSO and volumetric concentration containing volumetric concentration 1% The aqueous solution of 0.1% Tween 80,4 kinds of main pathogenic bacteria point spore reaping hooks that notoginseng root rot occurs for test Herba laggera volatile oil Bacterium (Fusarium oxysporum), Fusarium solani (Fusarium solani), rotten mould (Pythium Aphanidermatum) and the effect of column spore mould (Cylindrocarpon destructans) is damaged, and with leading to Radix Notoginseng 3 kinds of pathogenic bacteria botrytis cinereas (Botrytis cinerea), the colletotrichum gloeosporioides Penz (Colletotrichum of top disease generation Gloeosporioides), the effect of Rhizoctonia solani Kuhn (Rhizoctonia solani) mycelia growth；Laggera pterodonta as the result is shown Volatile oil has inhibiting effect to spore germination and mycelia growth, and Herba laggera volatile oil is compounded with chemical pesticide hymexazol, It was found that it all has collaboration and addition to the fungistatic effect of multiple bacterial strains.
Herba laggera volatile oil of the present invention is simple, easy to use with preparation method, investment is low, high-efficient, volatile, low The characteristics of poison, low-residual；In the case where country greatly develops the overall background of organic agriculture, Herba laggera volatile oil is more satisfactory to overcome The chemical agent substitute product of Radix Notoginseng disease are of great significance to the sustainable development of agricultural, have in agricultural production broader Application prospect.
Detailed description of the invention
Fig. 1 be Herba laggera volatile oil to Fusarium oxysporum (1), Fusarium solani (2), damage column spore mould (3), rotten mould (4), Botrytis cinerea (5), colletotrichum gloeosporioides Penz (6), Rhizoctonia solani Kuhn (7) bacterium colony growth influence result (colony diameter result)；
Fig. 2 is Herba laggera volatile oil to Fusarium oxysporum, Fusarium solani, damages that column spore is mould, rotten mould, botrytis cinerea, glue spore The bacteriostasis rate result that anthrax-bacilus, Rhizoctonia solani Kuhn bacterium colony are grown；
Fig. 3 is Radix Notoginseng seedling growth figure in Herba laggera volatile oil live test, and wherein a is Herba laggera volatile oil processing group；b For positive controls；C is negative control.
Specific embodiment
Below by drawings and examples, invention is further described in detail, but the scope of the present invention is not limited to The content, method is conventional method unless otherwise specified in embodiment, and the reagent used is routine unless otherwise specified Commercial reagent or the reagent prepared according to a conventional method.
Embodiment 1: the preparation of Herba laggera volatile oil
(1) water that 7 times of its quality is added in the laggera pterodonta of 1000g chopping, impregnates 10h, by the way of heating distillation, Using the volatile oil in steam extraction laggera pterodonta, extraction time 8h, condensation collects and slips out liquid；
(2) what is extracted slips out the drying of liquid anhydrous sodium sulfate, removes contained humidity to get Herba laggera volatile oil, puts Into in brown sample bottle, sealing, -20 DEG C are saved for use.
Embodiment 2: the activation of pathogenic bacteria
(1) potato dextrose agar (PDA): potato 200g, glucose 20g, fine jade 20g, distilled water 1000mL；
(2) Purification is carried out to the pathogenic bacteria for being isolated from Panax notoginseng root and overground part, is accredited as Fusarium oxysporum (Fusarium oxysporum), Fusarium solani (Fusarium solani), rotten mould (Pythium Aphanidermatum), column spore mould (Cylindrocarpon destructans), botrytis cinerea (Botrytis are damaged Cinerea), colletotrichum gloeosporioides Penz (Colletotrichum gloeosporioides) and Rhizoctonia solani Kuhn (Rhizoctonia Solani), above-mentioned strain is seeded in PDA culture medium and is activated, after inoculation 3~4 times, take eugonic bacterial strain standby With.
The chemical constituent of embodiment 3:GC-MS measurement Herba laggera volatile oil
(1) GC condition: instrument model: Agilengt Technologies 7890B-5977B；Chromatographic column: HP-5MS 30m×250μm×0.25μm；
(2) source EI source temperature: 230 DEG C；Level four bars temperature: 150 DEG C；Scanning range: 30~500m/z；Injection port temperature Degree: 285 DEG C；Split ratio 10:1；Sample volume: 1 μ l；Electron energy: 70eV；Column oven temperature program: 50 DEG C of holding 4min, 5 DEG C/ Min is warming up to 120 DEG C, and 1 DEG C/min is warming up to 180 DEG C, and 10 DEG C/min is warming up to 280 DEG C of holding 16min；Wherein RI value is according to just The retention time of structure alkane continuous carbon (C9-C25) is calculated, by the retention time of the compound and mass spectrum and NIST17.L data Library carries out comparing, determines final compound, test results in the following table:
Embodiment 4: the influence that measurement Herba laggera volatile oil grows 7 kinds of pathogenic bacteria mycelia in embodiment 2
(1) under aseptic technique, 15mL PDA culture medium is poured into each culture dish and takes reality after solidification to be cooled The pathogenic bacteria for cultivating 7d in example 2 in PDA culture medium are applied, is beaten with the punch of diameter 5mm along colony edge and takes fungus block, be inoculated in Culture medium central；
(2) 4 sterile Oxford cups are equidistantly placed at fungi block 25mm；
(3) DMSO and volumetric concentration of the Herba laggera volatile oil containing volumetric concentration 1% by embodiment 1 after dry The aqueous dissolution of 0.1% Tween 80, it is 50mg/mL Herba laggera volatile oil liquid that concentration, which is made,；
(4) the 50mg/mL Herba laggera volatile oil liquid of 200 μ L steps (3) is added in each Oxford cup (in advance with 0.22 μm organic system filter filtration sterilization), 28 DEG C of constant temperature incubations in microbiological incubator contain body be not added Herba laggera volatile oil The aqueous solution of the Tween 80 of the DMSO and volumetric concentration 0.1% of product concentration 1% is negative control, and hymexazol is positive control, often A processing sets 5 repetitions；
(5) after cultivating 7d in incubator, colony diameter is measured using " crossing method "；
The result is shown in Figure 1,2, it can be seen that concentration 50mg/mL laggera pterodonta oil is for causing Radix Notoginseng underground part to be sent out from Fig. 1,2 The inhibiting effect of the pathogenic bacteria (Fusarium oxysporum, Fusarium solani, breaking-up column spore are mould and rotten mould) of disease is weaker, and inhibiting rate exists 25% or less；For causing pathogenic bacteria botrytis cinerea, colletotrichum gloeosporioides Penz and the Rhizoctonia solani Kuhn of Radix Notoginseng aerial part disease, laggera pterodonta Careless volatile oil is stronger to the mycelia growth inhibitory effect of this 3 kinds of overground part pathogenic bacteria；The above result shows that the suppression of plant volatile oil Bacterium effect is influenced by the type of volatile oil itself composition and aimed strain.
Embodiment 5: Herba laggera volatile oil MIC measurement
(1) the Herba laggera volatile oil DMSO containing volumetric concentration 2% and volumetric concentration 1% by embodiment 1 after dry Tween 80 aqueous dissolution, the multiple concentration gradients for being 18.75-0.038mg/mL with doubling dilution configuration concentration range Liquid, positive drug hymexazol are configured to the liquid that concentration range is 18.75-0.038mg/mL with same method；
(2) pathogen that 7d is grown in embodiment 2, (does not add agar, potato with 20mL 1/4PDA fluid nutrient medium Amount with glucose is the 1/4 of conventional medium) culture medium is rinsed, it is 1 × 10 that spore concentration, which is made,4The spore suspension of a/mL Liquid；
(3) the 50 μ L Herba laggera volatile oils and 150 μ L steps of 0.22 μm of filtering with microporous membrane degerming are added in 96 orifice plates (2) manufactured spore suspension in；
(4) blank control be aqueous solution that 50 μ L contain 1% Tween 80 of volumetric concentration 2%DMSO and volumetric concentration and 150 μ L 1/4PDA fluid nutrient mediums；Positive control is that 50 μ L contain 1% Tween 80 of volumetric concentration 2%DMSO and volumetric concentration Spore suspension in aqueous solution and 150 μ L steps (2)；
(5) 96 28 DEG C of orifice plate constant temperature incubation 36h, then fungi growing state in inspection hole；Microplate reader is used at 595nm (Thermo 1510) measures the absorbance in each hole, as a result see the table below；
Note: the different letters of same row indicate that different disposal has significant difference to same bacterial strain
The minimal inhibitory concentration range of Wingedtooth Laggera Herb volatile oil is 0.24mg/mL-4.69mg/mL.
Embodiment 6: Herba laggera volatile oil and hymexazol synergy measure
(1) MIC according to embodiment 5 obtained Herba laggera volatile oil and hymexazol, being configured to maximum concentration is 8 times of MIC, Then 8 dilution gradients are configured using doubling dilution；
(2) by the combination addition of the hymexazol (25 μ L) of the Herba laggera volatile oil of various concentration (25 μ L) and various concentration 96 In orifice plate, manufactured spore suspension (150 μ L) in 5 step of embodiment (2) is then added in each hole；
In 5 step (2) of (3) 150 μ L embodiment manufactured spore suspension and 50 μ L contain volumetric concentration 1% DMSO and The aqueous solution of the Tween 80 of volumetric concentration 0.1% is positive control；
(4) 96 orifice plates save 36h in 28 DEG C of constant temperature, and with microplate reader (Thermo 1510) colorimetric at 595nm, detection is very Bacterium growing state；
(5) the MIC measurement of drug combination: corresponding drug concentration is grown as Herba laggera volatile oil using no fungi hole and is disliked MIC associated with mould spirit；
(6) FIC is calculated: the MIC of MIC/ volatile oil associated with the FIC=volatile oil and hymexazol of volatile oil, hymexazol The MIC of MIC/ hymexazol associated with FIC=volatile oil and hymexazol；
(7) FIC of the FIC+ hymexazol of FICI=volatile oil, wherein FICI≤0.5 is synergistic effect, 0.5 < FICI≤1 For addition, 1<FICI≤4 are unrelated effect, and FICI>4 are antagonism；FIC≤0.5, synergistic effect；0.5<FICI≤ 1, adduction；1 < FICI≤4, it is unrelated；FICI > 4, antagonism；As a result it see the table below；
For environmentally protective theory and resource-effective purpose that reduction chemical pesticide uses, development chessboard experiment allows smelly Miracle volatile oil and hymexazol interworking, Herba laggera volatile oil and hymexazol are used in combination in effect shown in table；For rotten mould and glue Spore anthrax-bacilus, Herba laggera volatile oil and hymexazol, which are used in combination, can generate synergy；And for Fusarium oxysporum, Beancurd sheet reaping hook Bacterium, breaking-up column spore are mould, and the fungistatic effect that can play adduction is used in combination in Herba laggera volatile oil and hymexazol；These results suggest that In actual production, Herba laggera volatile oil can press concentration proportioning appropriate with hymexazol, reduce the use of chemical pesticide, protection life State environment.
Embodiment 7: EC of the Herba laggera volatile oil to Fusarium oxysporum50Experiment
EC50 is to evaluate fungi to an important indicator of antimicrobial agent sensitivity, since Fusarium oxysporum is to cause Roots of Panax Notoginseng One of arch-criminal of maize ear rot, and it is more sensitive to Herba laggera volatile oil, therefore this experiment selects Fusarium oxysporum as test organisms, Experimental procedure is as follows:
(1) PDA culture medium is prepared, every part of 99mL is distributed into, is sealed, autoclave sterilization 20min；
(2) when PDA is cooled to 50 DEG C -55 DEG C, using sterile working, difference is separately added into every part of PDA culture medium The Fructus Foeniculi volatile oil 1mL of concentration, sufficiently shakes up, and obtains final concentration of 2MIC, MIC, 1/2MIC, 1/4MIC, 1/8MIC, 1/ The PDA solution of 16MIC；
(3) the above-mentioned PDA solution of 15mL is added in culture dish, repeats addition three times, it is past after PDA solution cooled and solidified The bacteria cake that center access diameter is 5mm, is placed in fungal culture case culture 7 days, measures colony diameter and calculates inhibiting rate, as a result sees Following table；
Upper table the results show that Herba laggera volatile oil EC50For 0.259mg/mL, the EC of positive drug hymexazol50For 0.021mg/mL；The above knot shows that 0.259mg/mL Herba laggera volatile oil can effectively inhibit half Fusarium oxysporum silk growth.
Embodiment 8: the live test of Herba laggera volatile oil
(1) Herba laggera volatile oil is added in the sterile culture matrix being made of vermiculite and quartz sand (volume ratio 2:1), The culture substrate (0.259mg/g) containing volatile oil is obtained, valve bag is sealed；
(2) after fumigating the culture substrate containing volatile oil 5 days, basin is filled by 250 grams of every basin of amounts；
(3) with 1 × 107After the Fusarium oxysporum spore suspension of a spore/mL impregnates Radix Notoginseng seedling 3 hours, every potting kind 10 plants, as Herba laggera volatile oil processing group, negative control group is immersion sterile water, while without the vermiculite of volatilization oil processing The Radix Notoginseng seedling impregnated with the sterile culture matrix plantation spore suspension of quartz sand (volume ratio 2:1) composition is as positive control Group；Every group of 5 repetitions；
(5) after cultivating 15 days, when apparent Disease symptoms occurs in positive controls seedling, to each group of Radix Notoginseng plant Root system, stalk, the fresh weight of blade and dry weight and plant height measure, and calculate the disease incidence and disease index of each group, as a result It see the table below and Fig. 3；
The different letters of same row indicate that different disposal has significant difference.

Claims (3)

1. application of the Herba laggera volatile oil in prevention and treatment Chinese medicine or crop root rot.
2. application of the Herba laggera volatile oil in the pathogenic bacteria that prevention and treatment causes Chinese medicine or crop overground part that disease occurs.
3. application according to claim 2, it is characterised in that: pathogenic bacteria are botrytis cinerea (Botrytis cinerea), glue Spore anthrax-bacilus (Colletotrichum gloeosporioides) and Rhizoctonia solani Kuhn (Rhizoctonia solani).
CN201910715508.2A 2019-08-05 2019-08-05 The new application of Herba laggera volatile oil Pending CN110250216A (en)

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Publication number Priority date Publication date Assignee Title
CN106106550A (en) * 2016-07-13 2016-11-16 彭水县渝宏清脆李种植股份合作社 A kind of compositions preventing and treating blue or green crisp plum red leaf spot
CN109287677A (en) * 2018-11-15 2019-02-01 云南中医学院 The purposes of flos caryophylli volatile oil
CN109452320A (en) * 2018-11-15 2019-03-12 云南中医学院 The purposes of Fructus Caryophylli volatile oil

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Title
YU-NAN MA 等: ""The beneficial use of essential oils from buds and fruit of Syzygium aromaticum to combat pathogenic fungi of Panax notoginseng"", 《INDUSTRIAL CROPS & PRODUCTS》 *
王险峰 等: "《新编植保实用技术》", 31 May 1998, 中国农业出版社 *
顾健龙 等: ""臭灵丹精油化学成分及其活性研究"", 《第十一届中国香料香精学术研讨会论文集》 *

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Application publication date: 20190920