CN105494460A - Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests - Google Patents
Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests Download PDFInfo
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- CN105494460A CN105494460A CN201510889588.5A CN201510889588A CN105494460A CN 105494460 A CN105494460 A CN 105494460A CN 201510889588 A CN201510889588 A CN 201510889588A CN 105494460 A CN105494460 A CN 105494460A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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Abstract
Application of melaleuca ahemifolia essential oil to control of stored-grain insect pests belongs to the technical field of biotechnology. According to the grain storage and production realities, the stored-grain insect pests (tribolium confusum and sitophilus zeamais) are repelled, touched out and fumigated. Compared with three essential oil single items and the mixing formula thereof, which are reported in the disclosed patent 201510340845.X, the melaleuca ahemifolia essential oil has a better control effect on the stored-grain insect pests (tribolium confusum and sitophilus zeamais), particularly has a remarkable fumigating insecticidal function, and is long in lasting period and low in probability of resistance to drugs. The melaleuca ahemifolia essential oil is rich in raw material source, low in cost and environmentally friendly, has the potential of being developed into a stored-grain insect pest fumigating agent, and has a very wide application prospect in controlling red-grain insect pests.
Description
Technical field
The invention belongs to biological technical field, particularly a kind of plant pesticide preventing and treating grain storage pest.
Background technology
Grain is the basis that the mankind depend on for existence, and maintain the key factor of national stability, China is the large agricultural country that world population is maximum.Foodstuff preservation is one of important means of Appropriate application grain resource, but the loss that the harm being but easily subject to grain storage pest between the storage life causes is very general and serious.According to relevant departments' statistics, due to the harm of grain storage pest, the grain storage of annual China grain depot loss reaches 0.2%, and farm storage is because to be simply equipped and Prevention Technique is not popularized, and grain storage loss is 8% ~ 10%.Therefore, the raising of grain storage technology and very urgent to the control of grain storage pest.
Be at present adopt chemical control for preventing and treating the topmost ways and means of grain storage pest in the world, but its " 3R " problem caused is day by day serious, the novel grain storage insecticide therefore developing a kind of environmental protection is significant.Based on current grain fumigating agent and protective agent institute problems faced, domestic and international scientific research personnel is devoted to start novel grain storage salvo.In chemical control, adopt vegetative insecticidal material more widely.As Chinese patent 201510262173.5 disclose Osthole and pepper extract, the built agent of stellera chamaejasme L extract has good action effect to corn weevil, confused flour beetle and lesser grain borer.Chinese patent 201510340845.X discloses and mixes composite Chinese cassia tree lavender environment-friendly pesticide by cinnamon essential oil, lemonlike citrus leaf essential oil and Lavender three kinds of essential oils, and this insecticide just has very outstanding toxic action to corn weevil at extremely low concentration.
Narrow leaved tea tree (Melaleucaahemifolia) essential oil be by the fresh branches and leaves of Myrtaceae paperbark narrow leaved tea tree obtain through steam distillation colourless to pale yellow oily liquid body essential oil, there is very high economic worth, be widely used in the fields such as medicine and commodity.
Chinese patent 201410004128.5 discloses the prevention and controls of narrow leaved tea tree essential oil to flameray gerbera insect pest.This invention will make 0.3% ~ 0.5% with water mixed liquor after narrow leaved tea tree essential oil ultrasonic emulsification, then fiting chemical combined use of pesticides is evenly sprayed on the blade of flameray gerbera, for preventing and treating acarid, Liriomyza, trialeurodes vaporariorum.Find through statistics, the use of narrow leaved tea tree essential oil greatly reduces the cost of pesticide control and the destruction to environment.
Chinese patent 201210163790.6 discloses a kind of tea oil bactericidal aqueous emulsion, preparation method and application.This invention is processed into aqueous emulsion using tea oil as main component and auxiliary agent, has good control efficiency to diseases of garden stuff such as graw mold of tomato, tomato wilt, Botrytis cinerea, grey mould fruit rot of strawberry, apple anthracnose, apple canker and pears scab.
Chinese patent 201510316789.6 discloses a kind of paddy field herbicidal composition.The active ingredient of this invention is made up of piperazine pyrazosulfuron, Evil humulone, pyribenzoxim and tea oil.This herbicidal composition has good insect killing effect to grassy weed, broad leaved weed and nutgrass flatsedge, has certain control efficiency to the soil-borne disease such as rice seedling blight, rice blast simultaneously, also has the effect of pest.The use of narrow leaved tea tree essential oil considerably reduces the use of agricultural chemicals.
As can be seen from above-mentioned execution example, narrow leaved tea tree essential oil has good broad-spectrum sterilization, desinsection and weeding isoreactivity.But narrow leaved tea tree essential oil, as the application of control grain storage pest insecticide, has had not yet to see bibliographical information.Therefore, narrow leaved tea tree essential oil has the potential quality being developed as plant pesticide, is a novel green plant pesticide being worth research and development.
Summary of the invention
The object of the present invention is to provide a kind of plant pesticide of novel control grain storage pest of efficient, environmental protection, i.e. the application of narrow leaved tea tree essential oil on control grain storage pest.
The present invention specify that narrow leaved tea tree essential oil has significant biologically active to two kinds of main grain storage pest confused flour beetles, corn weevil, and determine the effective dose of its control grain storage pest, for the novel grain storage pest insecticide developing environmental protection further provides scientific basis.
Object of the present invention is achieved through the following technical solutions:
1, described grain storage pest is confused flour beetle (Triboliumconfusum) and corn weevil (Sitophiluszeamais).
2, narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil repellent action.
3, narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil action of contace poison.
4, narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil fumigation action.
Narrow leaved tea tree essential oil described above, its preparation method, preferably prepare as follows:
Get the fresh branches and leaves chopping of plant narrow leaved tea tree, adopt water and steam distillation method to prepare.
The present invention compared with prior art, has following advantage:
1, narrow leaved tea tree essential oil of the present invention is a kind of vegetative insecticidal material, and it derives from natural world, and draw materials extensively, its secondary metabolites derives from plant itself and produces, and easily degrade, environmentally safe, do not pollute grain, insect also not easily develops immunity to drugs.
2, three kinds of essential oils reporting compared to disclosed patent 201510340845.X of the present invention and mixing formula thereof, have more excellent control efficiency, and the term of validity is long to grain storage pest confused flour beetle, corn weevil.
3, the present invention is particularly outstanding to the fumigating effect of grain storage pest confused flour beetle, corn weevil, has the potential being developed as grain storage pest fumigant.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, but the present invention is not limited to following examples.
Embodiment 1: narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil repellent action
1, for examination insect
Confused flour beetle: provided by Hua Zhong Agriculture University of Hubei Wuhan City for examination insect.With flour/Angel Yeast (19/1) for feed, temperature 30 ± 2 DEG C, relative moisture 75 ± 5%, carries out indoors artificial raising under dark condition, raises 5 generations more than continuously, selects and grows healthy adult as supplying examination worm source.
Corn weevil: provided by AnHui Agriculture University, HeFei City, AnHui Province for examination insect.With flour/Angel Yeast (19/1) for feed, temperature 30 ± 2 DEG C, relative moisture 75 ± 5%, carries out indoors artificial raising under dark condition, raises 5 generations more than continuously, selects and grows healthy adult as supplying examination worm source.
2, for examination narrow leaved tea tree essential oil:
Narrow leaved tea tree essential oil is commercial, purity is 92.68%, Contents of Main Components is 4-terpineol (terpinen-4-ol) 40.09%, γ-terpinene (γ-terpinene) 21.85%, α-terpinene (α-terpinene) 11.34%, alpha-terpineol (α-terpineol) 6.91%, australene (α-pinene) 5.86%, terpinolene (terpinolene) 3.24%, 1, 8-cineole (1, 8-cineole) 1.83%, limonene (limonene) 1.36%, p-cymene (p-cymene) 1.2%, sabinene (sabinene) 0.2%.
3, test method
Adopt filter paper medicine embrane method.Be that the filter paper of 9cm is cut into identical two halves by diameter, the liquid 0.4mL that half pipette pipettes variable concentrations respectively evenly notes on filter paper, under room temperature, acetone is volatilized; The acetone of second half instillation same volume, after volatilization is dry in contrast.Two halves filter paper is connected again, is fixedly placed in same diameter Petri dishes with viscose paper.Solid gum is smeared in ware primary coat, prevents examination worm from piercing below filter paper.By internal diameter 8cm, the glass ring that high 5cm inwall scribbles polytetrafluoroethylene (PTFE) is put in people's culture dish, prevents examination worm from swashing.Every ware accesses 30 examination worms, does not add feed in ware, adds that iron wire square prevents examination worm from escaping.Repeat 3 times.Foster worm incubator is put in each process, respectively at 24,48,72h time after the borer population on check processing limit and contrast limit, calculate scattering ratio.
The grade scale of scattering ratio:
0 grade: scattering ratio is 0; III grade: scattering ratio is 40.1 ~ 60%
I grade: scattering ratio is 0.1 ~ 20%; IV grade: scattering ratio is 60.1 ~ 80%
II grade: scattering ratio is 20.1 ~ 40%; V grade: scattering ratio is 80.1 ~ 100%
Table 1 narrow leaved tea tree essential oil is to the repellent action of corn weevil and confused flour beetle
As shown in Table 1: the present invention all has significant repellent action to confused flour beetle and corundum Sitophilusspp. imago, and dosage is higher, and its repellent action is larger; With maximum dose (1.57 μ L/cm
2) process is for examination adult, be 77.60%, 75.68% respectively to the average scattering ratio of confused flour beetle and corn weevil, its repellent grade is IV, the present invention is described to the repellent effect of confused flour beetle comparatively higher than corn weevil.Under same dose, along with the prolongation in processing time, the volatilization due to liquid causes concentration to decline, and makes repellent action entirety all present downward trend.
Embodiment 2: narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil action of contace poison
1, for examination insect
Supply examination insect confused flour beetle, corn weevil identical with embodiment 1.
2, for examination narrow leaved tea tree essential oil:
Identical with embodiment 1 for examination essential oil
3, test method
Adopt filter paper medicine embrane method.The filter paper of diameter 9cm is put into the culture dish of same diameter, filter paper is placed internal diameter 8cm, the glass ring that high 5cm inwall scribbles polytetrafluoroethylene (PTFE) prevents examination worm from swashing.With acetone, essential oil is diluted to the liquid of variable concentrations, and pipettes the liquid 1mL of each concentration respectively with liquid-transfering gun, instillation filter paper makes liquid be completely absorbed, after acetone volatilizees completely, by 30, every ware access examination worm, in ware, do not add feed, cover wire netting, prevent examination worm from escaping.Make blank with 1mL acetone, often process repetition 3 times.Foster worm incubator is put in each process, respectively at process 24,48,72h investigation examination worm death toll.Examination worm is touched without any reactor for death standard with writing brush.Calculate lethality, and use Abbott formula correction.
Table 2 narrow leaved tea tree essential oil is to the action of contace poison of corn weevil and confused flour beetle
Narrow leaved tea tree essential oil all has obvious action of contace poison to confused flour beetle and corn weevil as can be known from Table 2.After process 24h, concentration is 6.29 μ L/cm
2time, the lethality of confused flour beetle and corn weevil reaches 98.89%, 97.78% respectively, and contact efficacy all significantly strengthens along with the increase for the treatment of dosage; Under same treatment dosage, and its contact efficacy prolongation in time and strengthening gradually; After process 48h, the action of contace poison of narrow leaved tea tree essential oil to confused flour beetle and corn weevil all reaches 100%.
Embodiment 3: narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil fumigation action
1, for examination insect
Supply examination insect confused flour beetle, corn weevil identical with embodiment 1.
2, for examination narrow leaved tea tree essential oil:
Identical with embodiment 1 for examination essential oil
3, test method
Adopt wide-mouth bottle filter paper fumigating system.Examination worm 30 is only put into the wide-mouth bottle that volume is 300mL, and bottle inwall coats polytetrafluoroethylene (PTFE).The essential oil drawing variable concentrations with liquid-transfering gun respectively drips on 2 × 3cm filter paper bar, hangs in conical flask rapidly by filter paper bar, and filter paper bar can not contact with bottle wall, is stoppered bottle stopper sealing.Process is placed in 30 DEG C of constant incubators cultivates, and not add any reagent as blank.24,48, observe after 72h and record examination worm death condition in each bottle, calculate lethality and use Abbott formula correction.Each process repetition 3 times.
Table 3 narrow leaved tea tree essential oil is to the fumigation action of corn weevil and confused flour beetle
。
Narrow leaved tea tree essential oil all has obviously fumigation action to confused flour beetle and corn weevil as can be known from Table 3.After process 72h, when concentration is 13.33 μ L/L, the lethality of confused flour beetle and corn weevil reaches 98.86%, 92.05% respectively, and fumigating effect all significantly strengthens along with the increase for the treatment of dosage; Under same treatment dosage, and its fumigating effect prolongation in time and significantly strengthening.
Claims (7)
1. the application of narrow leaved tea tree essential oil on control grain storage pest.
2. narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil repellent action.
3. according to the narrow leaved tea tree essential oil of claim 2 to the application of grain storage pest confused flour beetle, corn weevil repellent action, it is characterized in that, narrow leaved tea tree essential oil consumption is 0.31-1.57 μ L/cm
2.
4. narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil action of contace poison.
5. according to the narrow leaved tea tree essential oil of claim 4 to the application of grain storage pest confused flour beetle, corn weevil action of contace poison, it is characterized in that, narrow leaved tea tree essential oil consumption is 4.72-6.29 μ L/cm
2.
6. narrow leaved tea tree essential oil is to the application of grain storage pest confused flour beetle, corn weevil fumigation action.
7. according to the narrow leaved tea tree essential oil of claim 6 to the application of grain storage pest confused flour beetle, corn weevil fumigation action, it is characterized in that, narrow leaved tea tree essential oil consumption is 7.00-13.33 μ L/L.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490037A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of plant source fumigant and preparation method thereof |
| CN107751252A (en) * | 2017-11-17 | 2018-03-06 | 中国林业科学研究院林产化学工业研究所 | Application of the Du Xiang branches and leaves total volatile oil in grain storage pest is prevented and treated |
| CN108432749A (en) * | 2018-03-28 | 2018-08-24 | 方建波 | A kind of preparation method of orchard bird repellent |
| CN109479904A (en) * | 2018-12-18 | 2019-03-19 | 安徽农业大学 | Middle mountain China fir essential oil and its application in agricultural insect pests control |
| CN110651797A (en) * | 2018-06-28 | 2020-01-07 | 云南森美达生物科技有限公司 | Plant extract, compound botanical insecticide, preparation method and application |
| CN111567520A (en) * | 2020-05-15 | 2020-08-25 | 南京林业大学 | Method for preventing and controlling adult willow leaf beetles and attractant used by method |
| TWI754092B (en) * | 2017-08-28 | 2022-02-01 | 澳大利亞商Ｃ＆Ｊ實驗室有限公司 | Monoterpene compositions and uses thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829074A (en) * | 2010-05-18 | 2010-09-15 | 南宁辰康生物科技有限公司 | Standardized recombinant tea tree oil and preparing method thereof |
| CN103689006A (en) * | 2014-01-06 | 2014-04-02 | 清流县鸿翔农庄农业发展有限公司 | Method for preventing gerbera jamesonii bolus pests by melaleuca ahemifolia oil |
-
2015
- 2015-12-02 CN CN201510889588.5A patent/CN105494460B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829074A (en) * | 2010-05-18 | 2010-09-15 | 南宁辰康生物科技有限公司 | Standardized recombinant tea tree oil and preparing method thereof |
| CN103689006A (en) * | 2014-01-06 | 2014-04-02 | 清流县鸿翔农庄农业发展有限公司 | Method for preventing gerbera jamesonii bolus pests by melaleuca ahemifolia oil |
Non-Patent Citations (3)
| Title |
|---|
| 刘国军: "植物精油及混用熏蒸剂对嗜卷书虱和赤拟谷盗的熏蒸控制作用", 《西南大学硕士学位论文》 * |
| 张海燕: "几种植物精油对长角扁谷盗成虫的熏蒸作用", 《植物保护》 * |
| 邓永学: "九种植物精油对玉米象成虫的熏蒸作用比较", 《农药学学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490037A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of plant source fumigant and preparation method thereof |
| TWI754092B (en) * | 2017-08-28 | 2022-02-01 | 澳大利亞商Ｃ＆Ｊ實驗室有限公司 | Monoterpene compositions and uses thereof |
| CN107751252A (en) * | 2017-11-17 | 2018-03-06 | 中国林业科学研究院林产化学工业研究所 | Application of the Du Xiang branches and leaves total volatile oil in grain storage pest is prevented and treated |
| CN107751252B (en) * | 2017-11-17 | 2020-07-03 | 中国林业科学研究院林产化学工业研究所 | Application of Ledum palustre branch and leaf total volatile oil in prevention and control of stored grain pests |
| CN108432749A (en) * | 2018-03-28 | 2018-08-24 | 方建波 | A kind of preparation method of orchard bird repellent |
| CN110651797A (en) * | 2018-06-28 | 2020-01-07 | 云南森美达生物科技有限公司 | Plant extract, compound botanical insecticide, preparation method and application |
| CN109479904A (en) * | 2018-12-18 | 2019-03-19 | 安徽农业大学 | Middle mountain China fir essential oil and its application in agricultural insect pests control |
| CN109479904B (en) * | 2018-12-18 | 2021-05-14 | 安徽农业大学 | Application of taxus chinensis essential oil in agricultural pest control |
| CN111567520A (en) * | 2020-05-15 | 2020-08-25 | 南京林业大学 | Method for preventing and controlling adult willow leaf beetles and attractant used by method |
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