CN105494460B - Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest - Google Patents

Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest Download PDF

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CN105494460B
CN105494460B CN201510889588.5A CN201510889588A CN105494460B CN 105494460 B CN105494460 B CN 105494460B CN 201510889588 A CN201510889588 A CN 201510889588A CN 105494460 B CN105494460 B CN 105494460B
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essential oil
grain storage
tea tree
storage pest
tree essential
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CN105494460A (en
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操海群
廖敏
肖金京
蒋兴川
周丽君
姚曦
汤锋
岳永德
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Anhui Agricultural University AHAU
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/28Myrtaceae [Myrtle family], e.g. teatree or clove

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

A kind of application of the narrow leaved tea tree essential oil as plant pesticide on preventing and treating grain storage pest, belongs to biological technical field.The present invention combines foodstuff preservation produce reality, grain storage pest confused flour beetle and sitophilus zea-mais are carried out repellent, tag, fumigation action, compared with the 3 kinds of essential oil list product and its mixing formula reported in disclosed patent 201510340845.X, the present invention is more preferable to the prevention effect of grain storage pest confused flour beetle and sitophilus zea-mais, especially fumigating insect killing action is notable, and the lasting period is long, the resistance to the action of a drug is not likely to produce.Narrow leaved tea tree essential oil raw material resources of the invention are enriched, low cost, environmental protection, are the potential of grain storage pest fumigant with exploitation, there is boundless application prospect in terms of the preventing and treating of grain storage pest.

Description

Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest
Technical field
The invention belongs to biological technical field, more particularly to a kind of plant pesticide for preventing and treating grain storage pest.
Background technology
Grain is basis for the survival of mankind, maintains the key factor of national stability, and China is most world population Large agricultural country.Foodstuff preservation is one of important means rationally using grain resource, but is but easily subject to during storing Loss caused by the harm of grain storage pest is very universal and serious.Counted according to relevant departments, due to the harm of grain storage pest, often The year grain storage of China's grain depot is lost up to 0.2%, and farm storage do not popularized with Prevention Technique because being simply equipped, and grain storage loss is 8%~10%.Therefore, the raising of grain storage technology and very urgent to the preventing and treating of grain storage pest.
The topmost ways and means for being used to prevent and treat grain storage pest in the world at present is to use chemical prevention, but it causes " 3R " problem it is increasingly serious, therefore develop it is a kind of environmental protection novel grain storage insecticide it is significant.Based on current grain Fumigant and protective agent problem encountered, domestic and international scientific research personnel are devoted to starting new grain storage salvo.Medicament is prevented Control, wide use vegetative insecticidal material.As Chinese patent 201510262173.5 discloses Osthole and flower Green pepper extract, the built agent of stellera chamaejasme L extract have good action effect to sitophilus zea-mais, confused flour beetle and lesser grain borer.China Patent 201510340845.X is disclosed by cinnamon essential oil, three kinds of meat of essential oil mixing compounding of lemon leaf essential oil and Lavender Osmanthus lavender environment-friendly pesticide, the insecticide just has extremely outstanding toxic action in extremely low concentration to sitophilus zea-mais.
Narrow leaved tea tree (Melaleuca ahemifolia) essential oil is by the mutual leaf of Myrtaceae paperbark white thousand It is colourless to pale yellow oily liquid essential oil that the fresh branches and leaves of layer are obtained through steam distillation, with economic worth very high, It is widely used in the fields such as medicine and commodity.
Chinese patent 201410004128.5 discloses prevention and controls of the narrow leaved tea tree essential oil to African Chrysanthemum insect pest.The hair 0.3%~0.5% mixed liquor is made after the bright essential oil ultrasonic emulsification by narrow leaved tea tree of water, then fiting chemical combined use of pesticides Uniformly it is sprayed on the blade of African Chrysanthemum, for preventing and treating acarid, Liriomyza, trialeurodes vaporariorum.Found through statistics, narrow leaved tea tree essential oil Use greatly reduce the cost of pesticide control and the destruction to environment.
Chinese patent 201210163790.6 discloses a kind of tea oil bactericidal aqueous emulsion, preparation method and application.The hair It is bright that aqueous emulsion is processed into as main component and auxiliary agent using tea oil, to graw mold of tomato, tomato wilt, Botrytis cinerea, The diseases of garden stuff such as grey mould fruit rot of strawberry, apple anthracnose, canker of apple fruit and pear scab have good prevention effect.
Chinese patent 201510316789.6 discloses a kind of rice terrace herbicidal composition.The active ingredient of the invention It is made up of piperazine pyrazosulfuron, Evil humulones, pyribenzoxim and tea oil.The herbicidal composition is miscellaneous to grassy weed, broad-leaved Grass and nutgrass flatsedge have preferable insect killing effect, while having certain preventing and treating to imitate to soil-borne diseases such as rice seedling blight, rice blast Really, the also effect with pest.The use of narrow leaved tea tree essential oil considerably reduces the use of agricultural chemicals.
From above-mentioned execution example as can be seen that narrow leaved tea tree essential oil has good broad-spectrum sterilization, desinsection and weeding etc. living Property.However, narrow leaved tea tree essential oil has had not yet to see document report as the application of preventing and treating grain storage pest insecticide.Therefore, It is the potential quality of plant pesticide that narrow leaved tea tree essential oil has exploitation, and being one is worth the novel green plant pesticide of research and development.
The content of the invention
It is an object of the invention to provide it is a kind of efficiently, the plant pesticide of the new preventing and treating grain storage pest of environmental protection, i.e., Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest.
It is notable that the present invention specify that the narrow leaved tea tree essential oil grain storage pest confused flour beetle main to two kinds, sitophilus zea-mais have Bioactivity, and determine its preventing and treating grain storage pest effective dose, be further develop environmental protection novel grain storage pest Insecticide provides scientific basis.
The purpose of the present invention is achieved through the following technical solutions:
1st, the grain storage pest is confused flour beetle (Tribolium confusum) and sitophilus zea-mais (Sitophilus zeamais)。
2nd, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais repellent action.
3rd, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais action of contace poison.
4th, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais fumigation action.
Narrow leaved tea tree essential oil described above, its preparation method is preferably prepared as follows:
The fresh branches and leaves chopping of plant narrow leaved tea tree is taken, is prepared using water and steam distillation method.
Compared with prior art, tool has the advantage that the present invention:
1st, narrow leaved tea tree essential oil of the invention is a kind of vegetative insecticidal material, and it derives from nature, and materials are extensive, Its secondary metabolites is produced in itself from plant, degradable, environmentally safe, does not pollute grain, and insect is also less prone to produce The resistance to the action of a drug.
2nd, the three kinds of essential oils and its mixing formula reported compared to disclosed patent 201510340845.X of the invention, to storage Grain insect confused flour beetle, sitophilus zea-mais have more excellent prevention effect, and the term of validity is long.
3rd, the present invention is especially prominent to the fumigating effect of grain storage pest confused flour beetle, sitophilus zea-mais, with exploitation for grain storage does harm to The potential of worm fumigant.
Specific embodiment
With reference to embodiment, the present invention will be further described, but the present invention is not limited to following examples.
Embodiment 1:Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais repellent action
1st, for examination insect
Confused flour beetle:There is provided by Hua Zhong Agriculture University of Hubei Wuhan City for examination insect.It is with flour/Angel Yeast (19/1) Feed, in 30 ± 2 DEG C of temperature, relative humidity 75 ± 5% carries out indoors artificial raising under dark condition, it is continuous raise 5 generations with On, the adult of development health is selected as selected insect source.
Sitophilus zea-mais:There is provided by AnHui Agriculture University, HeFei City, AnHui Province for examination insect.It is with flour/Angel Yeast (19/1) Feed, in 30 ± 2 DEG C of temperature, relative humidity 75 ± 5% carries out indoors artificial raising under dark condition, it is continuous raise 5 generations with On, the adult of development health is selected as selected insect source.
2nd, for examination narrow leaved tea tree essential oil:
Narrow leaved tea tree essential oil is purchased in market, and purity is 92.68%, and Contents of Main Components is 4- terpinols (terpinen-4- Ol) 40.09%, γ-terpinene (γ-terpinene) 21.85%, α-terpinene (α-terpinene) 11.34%, α-pine tar Alcohol (α-terpineol) 6.91%, australene (α-pinene) 5.86%, terpinolene (terpinolene) 3.24%, 1,8- , limonenes (limonene) 1.36%, p-cymene (p-cymene) 1.2%, sabinene of cineole (1,8-cineole) 1.83% (sabinene) 0.2%.
3rd, test method
Using filter paper medicine embrane method.The filter paper of a diameter of 9cm is cut into identical two halves, half pipette pipettes difference respectively The liquid 0.4mL of concentration is uniform to be noted on filter paper, making acetone volatilize at room temperature;Second half instills the acetone of same volume, and volatilization is dry Afterwards as control.Two halves filter paper is connected again, is placed in after being fixed with viscose paper in same diameter Petri dishes.Solid is smeared in ware primary coat Glue, prevents test worm from piercing below filter paper.Internal diameter 8cm, the glass ring that 5cm inwalls high scribble polytetrafluoroethylene (PTFE) are put into people's culture dish In, prevent test worm from swashing.30 test worms are accessed per ware, feed is not added with ware, adding iron wire square prevents test worm from escaping.Repeat 3 It is secondary.By it is each treatment be put into foster worm incubator, when 24,48,72 h after check treatment while and control while borer population, calculate Scattering ratio.
The grade scale of scattering ratio:
0 grade:Scattering ratio is 0;III grade:Scattering ratio is 40.1~60%
I grade:Scattering ratio is 0.1~20%;IV grade:Scattering ratio is 60.1~80%
II grade:Scattering ratio is 20.1~40%;V grade:Scattering ratio is 80.1~100%
The narrow leaved tea tree essential oil of table 1 is to sitophilus zea-mais and the repellent action of confused flour beetle
As shown in Table 1:The present invention is respectively provided with significant repellent action to confused flour beetle and corundum Sitophilusspp. imago, and dosage is got over Height, its repellent action is bigger;With maximum dose (1.57 μ L/cm2) treatment for examination adult, to the average of confused flour beetle and sitophilus zea-mais Scattering ratio is respectively 77.60%, 75.68%, and its repellent grade is IV, illustrate the present invention to the repellent effect of confused flour beetle compared with Higher than sitophilus zea-mais.Under same dose, with the extension of process time, because the volatilization of liquid causes concentration to decline so that drive The effect of keeping away is overall to be presented downward trend.
Embodiment 2:Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais action of contace poison
1st, for examination insect
It is identical with embodiment 1 for examination insect confused flour beetle, sitophilus zea-mais.
2nd, for examination narrow leaved tea tree essential oil:
It is identical with embodiment 1 for examination essential oil
3rd, test method
Using filter paper medicine embrane method.The filter paper of diameter 9cm is put into the culture dish of same diameter, internal diameter is placed on filter paper 8cm, the glass ring that 5cm inwalls high scribble polytetrafluoroethylene (PTFE) prevents test worm from swashing.Essential oil is diluted to various concentrations with acetone Liquid, and pipette the liquid 1mL of each concentration respectively with liquid-transfering gun, to instill and be completely absorbed liquid on filter paper, treat that acetone is complete After volatilization, by 30 access test worms of every ware, feed is not added with ware, covers wire netting, prevent test worm from escaping.Make empty with 1mL acetone White control, often treatment is repeated 3 times.Each treatment is put into foster worm incubator, it is dead respectively at treatment 24,48,72h investigation test worm Number.Test worm is touched without any reactor as death standard with writing brush.The death rate is calculated, and uses Abbott formula corrections.
Action of contace poison of the narrow leaved tea tree essential oil of table 2 to sitophilus zea-mais and confused flour beetle
Narrow leaved tea tree essential oil all has obvious action of contace poison to confused flour beetle and sitophilus zea-mais as can be known from Table 2.Treatment After 24h, concentration is 6.29 μ L/cm2When, the death rate of confused flour beetle and sitophilus zea-mais respectively reaches 98.89%, 97.78%, tags Effect is significantly increased with the increase for the treatment of dosage;Under same treatment dosage, and its contact efficacy is with the extension of time And gradually strengthen;After treatment 48h, action of contace poison of the narrow leaved tea tree essential oil to confused flour beetle and sitophilus zea-mais reaches 100%.
Embodiment 3:Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais fumigation action
1st, for examination insect
It is identical with embodiment 1 for examination insect confused flour beetle, sitophilus zea-mais.
2nd, for examination narrow leaved tea tree essential oil:
It is identical with embodiment 1 for examination essential oil
3rd, test method
Using wide-mouth bottle filter paper fumigating system.Test worm 30 is only put into the wide-mouth bottle that volume is 300mL, bottle inwall is coated poly- Tetrafluoroethene.The essential oil for drawing various concentrations respectively with liquid-transfering gun is dripped on 2 × 3cm filter paper bars, rapidly hangs on filter paper bar In conical flask, filter paper bar can not be contacted with bottle wall, be stoppered bottle stopper sealing.It is placed in after treatment in 30 DEG C of constant incubators and is cultivated, And to be not added with any reagent as blank.24th, 48, observed after 72h and record test worm death condition in each bottle, calculate dead Rate and use Abbott formula corrections.Each treatment is repeated 3 times.
Fumigation action of the narrow leaved tea tree essential oil of table 3 to sitophilus zea-mais and confused flour beetle
Narrow leaved tea tree essential oil all has obviously fumigation action to confused flour beetle and sitophilus zea-mais as can be known from Table 3. After treatment 72h, when concentration is 13.33 μ L/L, the death rate of confused flour beetle and sitophilus zea-mais respectively reaches 98.86%, 92.05%, Fumigating effect is significantly increased with the increase for the treatment of dosage;Under same treatment dosage, and its fumigating effect is with the time Extend and significantly increase.

Claims (2)

1. application of the narrow leaved tea tree essential oil to grain storage pest confused flour beetle action of contace poison, narrow leaved tea tree essential oil main component matter Measuring percentage composition is:4- terpinols 40.09%, γ-terpinene 21.85%, α-terpinene 11.34%, alpha-terpineol 6.91%, Australene 5.86%, terpinolene 3.24%, the, limonenes 1.36% of 1,8- cineoles 1.83%, p-cymene 1.2%, sabinene 0.2%.
2. the application according to the narrow leaved tea tree essential oil of claim 1 to grain storage pest confused flour beetle action of contace poison, its feature exists In narrow leaved tea tree essential oil consumption is 4.72-6.29 μ L/cm2, confused flour beetle is continuous raising 5 more than generation.
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CN107751252B (en) * 2017-11-17 2020-07-03 中国林业科学研究院林产化学工业研究所 Application of Ledum palustre branch and leaf total volatile oil in prevention and control of stored grain pests
CN108432749A (en) * 2018-03-28 2018-08-24 方建波 A kind of preparation method of orchard bird repellent
CN110651797A (en) * 2018-06-28 2020-01-07 云南森美达生物科技有限公司 Plant extract, compound botanical insecticide, preparation method and application
CN109479904B (en) * 2018-12-18 2021-05-14 安徽农业大学 Application of taxus chinensis essential oil in agricultural pest control
CN111567520B (en) * 2020-05-15 2021-09-03 南京林业大学 Method for preventing and controlling adult willow leaf beetles and attractant used by method

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