CN105494460B - Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest - Google Patents
Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest Download PDFInfo
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- CN105494460B CN105494460B CN201510889588.5A CN201510889588A CN105494460B CN 105494460 B CN105494460 B CN 105494460B CN 201510889588 A CN201510889588 A CN 201510889588A CN 105494460 B CN105494460 B CN 105494460B
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- tea tree
- storage pest
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 46
- 235000009024 Ceanothus sanguineus Nutrition 0.000 title claims abstract description 37
- 240000003553 Leptospermum scoparium Species 0.000 title claims abstract description 37
- 235000015459 Lycium barbarum Nutrition 0.000 title claims abstract description 37
- 238000003860 storage Methods 0.000 title claims abstract description 36
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 30
- 235000013339 cereals Nutrition 0.000 claims abstract description 39
- 241000254112 Tribolium confusum Species 0.000 claims abstract description 31
- 230000009471 action Effects 0.000 claims abstract description 20
- 239000002574 poison Substances 0.000 claims description 7
- 231100000614 poison Toxicity 0.000 claims description 7
- MOYAFQVGZZPNRA-UHFFFAOYSA-N Terpinolene Chemical compound CC(C)=C1CCC(C)=CC1 MOYAFQVGZZPNRA-UHFFFAOYSA-N 0.000 claims description 6
- NDVASEGYNIMXJL-UHFFFAOYSA-N sabinene Chemical compound C=C1CCC2(C(C)C)C1C2 NDVASEGYNIMXJL-UHFFFAOYSA-N 0.000 claims description 6
- YHQGMYUVUMAZJR-UHFFFAOYSA-N α-terpinene Chemical compound CC(C)C1=CC=C(C)CC1 YHQGMYUVUMAZJR-UHFFFAOYSA-N 0.000 claims description 6
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- NDVASEGYNIMXJL-NXEZZACHSA-N (+)-sabinene Natural products C=C1CC[C@@]2(C(C)C)[C@@H]1C2 NDVASEGYNIMXJL-NXEZZACHSA-N 0.000 claims description 3
- WSTYNZDAOAEEKG-UHFFFAOYSA-N Mayol Natural products CC1=C(O)C(=O)C=C2C(CCC3(C4CC(C(CC4(CCC33C)C)=O)C)C)(C)C3=CC=C21 WSTYNZDAOAEEKG-UHFFFAOYSA-N 0.000 claims description 3
- KQAZVFVOEIRWHN-UHFFFAOYSA-N alpha-thujene Natural products CC1=CCC2(C(C)C)C1C2 KQAZVFVOEIRWHN-UHFFFAOYSA-N 0.000 claims description 3
- 235000001510 limonene Nutrition 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229930006696 sabinene Natural products 0.000 claims description 3
- WUOACPNHFRMFPN-SECBINFHSA-N (S)-(-)-alpha-terpineol Chemical compound CC1=CC[C@@H](C(C)(C)O)CC1 WUOACPNHFRMFPN-SECBINFHSA-N 0.000 claims description 2
- OVKDFILSBMEKLT-UHFFFAOYSA-N alpha-Terpineol Natural products CC(=C)C1(O)CCC(C)=CC1 OVKDFILSBMEKLT-UHFFFAOYSA-N 0.000 claims description 2
- 229940088601 alpha-terpineol Drugs 0.000 claims description 2
- 150000002628 limonene derivativess Chemical class 0.000 claims description 2
- HFPZCAJZSCWRBC-UHFFFAOYSA-N p-cymene Chemical compound CC(C)C1=CC=C(C)C=C1 HFPZCAJZSCWRBC-UHFFFAOYSA-N 0.000 claims 2
- 150000000193 1,8-cineol derivatives Chemical class 0.000 claims 1
- 241000254154 Sitophilus zeamais Species 0.000 abstract description 28
- 241000238631 Hexapoda Species 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 11
- 239000000575 pesticide Substances 0.000 abstract description 8
- 239000005871 repellent Substances 0.000 abstract description 8
- 230000002940 repellent Effects 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 6
- 230000002265 prevention Effects 0.000 abstract description 6
- 238000003958 fumigation Methods 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 239000002316 fumigant Substances 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract description 2
- 230000002147 killing effect Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 230000002045 lasting effect Effects 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000006378 damage Effects 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- WEEGYLXZBRQIMU-UHFFFAOYSA-N Eucalyptol Chemical compound C1CC2CCC1(C)OC2(C)C WEEGYLXZBRQIMU-UHFFFAOYSA-N 0.000 description 3
- -1 Evil humulones Chemical compound 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 239000010495 camellia oil Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- GRWFGVWFFZKLTI-IUCAKERBSA-N (-)-α-pinene Chemical compound CC1=CC[C@@H]2C(C)(C)[C@H]1C2 GRWFGVWFFZKLTI-IUCAKERBSA-N 0.000 description 2
- WRYLYDPHFGVWKC-UHFFFAOYSA-N 4-terpineol Chemical compound CC(C)C1(O)CCC(C)=CC1 WRYLYDPHFGVWKC-UHFFFAOYSA-N 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 240000005250 Chrysanthemum indicum Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
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- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000001256 steam distillation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OVXMBIVWNJDDSM-UHFFFAOYSA-N (benzhydrylideneamino) 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoate Chemical compound COC1=CC(OC)=NC(OC=2C(=C(OC=3N=C(OC)C=C(OC)N=3)C=CC=2)C(=O)ON=C(C=2C=CC=CC=2)C=2C=CC=CC=2)=N1 OVXMBIVWNJDDSM-UHFFFAOYSA-N 0.000 description 1
- RLXAQIXESOWNGY-UHFFFAOYSA-N 1-methyl-4-propan-2-ylbenzene Chemical compound CC(C)C1=CC=C(C)C=C1.CC(C)C1=CC=C(C)C=C1 RLXAQIXESOWNGY-UHFFFAOYSA-N 0.000 description 1
- WRYLYDPHFGVWKC-SNVBAGLBSA-N 4-Terpineol Natural products CC(C)[C@]1(O)CCC(C)=CC1 WRYLYDPHFGVWKC-SNVBAGLBSA-N 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 235000002848 Cyperus flabelliformis Nutrition 0.000 description 1
- 240000001505 Cyperus odoratus Species 0.000 description 1
- 244000075634 Cyperus rotundus Species 0.000 description 1
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 241000594036 Liriomyza Species 0.000 description 1
- 241000378467 Melaleuca Species 0.000 description 1
- 244000203416 Melaleuca leucadendron Species 0.000 description 1
- 235000001136 Melaleuca leucadendron Nutrition 0.000 description 1
- 241000219926 Myrtaceae Species 0.000 description 1
- 241000190142 Neofusicoccum ribis Species 0.000 description 1
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 description 1
- VXMNDQDDWDDKOQ-UHFFFAOYSA-N Pyrazosulfuron Chemical compound COC1=CC(OC)=NC(NC(=O)NS(=O)(=O)C=2N(N=CC=2C(O)=O)C)=N1 VXMNDQDDWDDKOQ-UHFFFAOYSA-N 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 241000318997 Rhyzopertha dominica Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 235000005010 Scirpus paludosus Nutrition 0.000 description 1
- 241001263604 Stellera chamaejasme Species 0.000 description 1
- 241000018137 Trialeurodes vaporariorum Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- MVNCAPSFBDBCGF-UHFFFAOYSA-N alpha-pinene Natural products CC1=CCC23C1CC2C3(C)C MVNCAPSFBDBCGF-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- RFFOTVCVTJUTAD-UHFFFAOYSA-N cineole Natural products C1CC2(C)CCC1(C(C)C)O2 RFFOTVCVTJUTAD-UHFFFAOYSA-N 0.000 description 1
- 229960005233 cineole Drugs 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 239000010431 corundum Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229940087305 limonene Drugs 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 description 1
- 229940116257 pepper extract Drugs 0.000 description 1
- 239000011297 pine tar Substances 0.000 description 1
- 229940068124 pine tar Drugs 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- GRWFGVWFFZKLTI-UHFFFAOYSA-N rac-alpha-Pinene Natural products CC1=CCC2C(C)(C)C1C2 GRWFGVWFFZKLTI-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
A kind of application of the narrow leaved tea tree essential oil as plant pesticide on preventing and treating grain storage pest, belongs to biological technical field.The present invention combines foodstuff preservation produce reality, grain storage pest confused flour beetle and sitophilus zea-mais are carried out repellent, tag, fumigation action, compared with the 3 kinds of essential oil list product and its mixing formula reported in disclosed patent 201510340845.X, the present invention is more preferable to the prevention effect of grain storage pest confused flour beetle and sitophilus zea-mais, especially fumigating insect killing action is notable, and the lasting period is long, the resistance to the action of a drug is not likely to produce.Narrow leaved tea tree essential oil raw material resources of the invention are enriched, low cost, environmental protection, are the potential of grain storage pest fumigant with exploitation, there is boundless application prospect in terms of the preventing and treating of grain storage pest.
Description
Technical field
The invention belongs to biological technical field, more particularly to a kind of plant pesticide for preventing and treating grain storage pest.
Background technology
Grain is basis for the survival of mankind, maintains the key factor of national stability, and China is most world population
Large agricultural country.Foodstuff preservation is one of important means rationally using grain resource, but is but easily subject to during storing
Loss caused by the harm of grain storage pest is very universal and serious.Counted according to relevant departments, due to the harm of grain storage pest, often
The year grain storage of China's grain depot is lost up to 0.2%, and farm storage do not popularized with Prevention Technique because being simply equipped, and grain storage loss is
8%~10%.Therefore, the raising of grain storage technology and very urgent to the preventing and treating of grain storage pest.
The topmost ways and means for being used to prevent and treat grain storage pest in the world at present is to use chemical prevention, but it causes
" 3R " problem it is increasingly serious, therefore develop it is a kind of environmental protection novel grain storage insecticide it is significant.Based on current grain
Fumigant and protective agent problem encountered, domestic and international scientific research personnel are devoted to starting new grain storage salvo.Medicament is prevented
Control, wide use vegetative insecticidal material.As Chinese patent 201510262173.5 discloses Osthole and flower
Green pepper extract, the built agent of stellera chamaejasme L extract have good action effect to sitophilus zea-mais, confused flour beetle and lesser grain borer.China
Patent 201510340845.X is disclosed by cinnamon essential oil, three kinds of meat of essential oil mixing compounding of lemon leaf essential oil and Lavender
Osmanthus lavender environment-friendly pesticide, the insecticide just has extremely outstanding toxic action in extremely low concentration to sitophilus zea-mais.
Narrow leaved tea tree (Melaleuca ahemifolia) essential oil is by the mutual leaf of Myrtaceae paperbark white thousand
It is colourless to pale yellow oily liquid essential oil that the fresh branches and leaves of layer are obtained through steam distillation, with economic worth very high,
It is widely used in the fields such as medicine and commodity.
Chinese patent 201410004128.5 discloses prevention and controls of the narrow leaved tea tree essential oil to African Chrysanthemum insect pest.The hair
0.3%~0.5% mixed liquor is made after the bright essential oil ultrasonic emulsification by narrow leaved tea tree of water, then fiting chemical combined use of pesticides
Uniformly it is sprayed on the blade of African Chrysanthemum, for preventing and treating acarid, Liriomyza, trialeurodes vaporariorum.Found through statistics, narrow leaved tea tree essential oil
Use greatly reduce the cost of pesticide control and the destruction to environment.
Chinese patent 201210163790.6 discloses a kind of tea oil bactericidal aqueous emulsion, preparation method and application.The hair
It is bright that aqueous emulsion is processed into as main component and auxiliary agent using tea oil, to graw mold of tomato, tomato wilt, Botrytis cinerea,
The diseases of garden stuff such as grey mould fruit rot of strawberry, apple anthracnose, canker of apple fruit and pear scab have good prevention effect.
Chinese patent 201510316789.6 discloses a kind of rice terrace herbicidal composition.The active ingredient of the invention
It is made up of piperazine pyrazosulfuron, Evil humulones, pyribenzoxim and tea oil.The herbicidal composition is miscellaneous to grassy weed, broad-leaved
Grass and nutgrass flatsedge have preferable insect killing effect, while having certain preventing and treating to imitate to soil-borne diseases such as rice seedling blight, rice blast
Really, the also effect with pest.The use of narrow leaved tea tree essential oil considerably reduces the use of agricultural chemicals.
From above-mentioned execution example as can be seen that narrow leaved tea tree essential oil has good broad-spectrum sterilization, desinsection and weeding etc. living
Property.However, narrow leaved tea tree essential oil has had not yet to see document report as the application of preventing and treating grain storage pest insecticide.Therefore,
It is the potential quality of plant pesticide that narrow leaved tea tree essential oil has exploitation, and being one is worth the novel green plant pesticide of research and development.
The content of the invention
It is an object of the invention to provide it is a kind of efficiently, the plant pesticide of the new preventing and treating grain storage pest of environmental protection, i.e.,
Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest.
It is notable that the present invention specify that the narrow leaved tea tree essential oil grain storage pest confused flour beetle main to two kinds, sitophilus zea-mais have
Bioactivity, and determine its preventing and treating grain storage pest effective dose, be further develop environmental protection novel grain storage pest
Insecticide provides scientific basis.
The purpose of the present invention is achieved through the following technical solutions：
1st, the grain storage pest is confused flour beetle (Tribolium confusum) and sitophilus zea-mais (Sitophilus
zeamais)。
2nd, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais repellent action.
3rd, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais action of contace poison.
4th, narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais fumigation action.
Narrow leaved tea tree essential oil described above, its preparation method is preferably prepared as follows：
The fresh branches and leaves chopping of plant narrow leaved tea tree is taken, is prepared using water and steam distillation method.
Compared with prior art, tool has the advantage that the present invention:
1st, narrow leaved tea tree essential oil of the invention is a kind of vegetative insecticidal material, and it derives from nature, and materials are extensive,
Its secondary metabolites is produced in itself from plant, degradable, environmentally safe, does not pollute grain, and insect is also less prone to produce
The resistance to the action of a drug.
2nd, the three kinds of essential oils and its mixing formula reported compared to disclosed patent 201510340845.X of the invention, to storage
Grain insect confused flour beetle, sitophilus zea-mais have more excellent prevention effect, and the term of validity is long.
3rd, the present invention is especially prominent to the fumigating effect of grain storage pest confused flour beetle, sitophilus zea-mais, with exploitation for grain storage does harm to
The potential of worm fumigant.
Specific embodiment
With reference to embodiment, the present invention will be further described, but the present invention is not limited to following examples.
Embodiment 1：Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais repellent action
1st, for examination insect
Confused flour beetle：There is provided by Hua Zhong Agriculture University of Hubei Wuhan City for examination insect.It is with flour/Angel Yeast (19/1)
Feed, in 30 ± 2 DEG C of temperature, relative humidity 75 ± 5% carries out indoors artificial raising under dark condition, it is continuous raise 5 generations with
On, the adult of development health is selected as selected insect source.
Sitophilus zea-mais：There is provided by AnHui Agriculture University, HeFei City, AnHui Province for examination insect.It is with flour/Angel Yeast (19/1)
Feed, in 30 ± 2 DEG C of temperature, relative humidity 75 ± 5% carries out indoors artificial raising under dark condition, it is continuous raise 5 generations with
On, the adult of development health is selected as selected insect source.
2nd, for examination narrow leaved tea tree essential oil：
Narrow leaved tea tree essential oil is purchased in market, and purity is 92.68%, and Contents of Main Components is 4- terpinols (terpinen-4-
Ol) 40.09%, γ-terpinene (γ-terpinene) 21.85%, α-terpinene (α-terpinene) 11.34%, α-pine tar
Alcohol (α-terpineol) 6.91%, australene (α-pinene) 5.86%, terpinolene (terpinolene) 3.24%, 1,8-
, limonenes (limonene) 1.36%, p-cymene (p-cymene) 1.2%, sabinene of cineole (1,8-cineole) 1.83%
(sabinene) 0.2%.
3rd, test method
Using filter paper medicine embrane method.The filter paper of a diameter of 9cm is cut into identical two halves, half pipette pipettes difference respectively
The liquid 0.4mL of concentration is uniform to be noted on filter paper, making acetone volatilize at room temperature；Second half instills the acetone of same volume, and volatilization is dry
Afterwards as control.Two halves filter paper is connected again, is placed in after being fixed with viscose paper in same diameter Petri dishes.Solid is smeared in ware primary coat
Glue, prevents test worm from piercing below filter paper.Internal diameter 8cm, the glass ring that 5cm inwalls high scribble polytetrafluoroethylene (PTFE) are put into people's culture dish
In, prevent test worm from swashing.30 test worms are accessed per ware, feed is not added with ware, adding iron wire square prevents test worm from escaping.Repeat 3
It is secondary.By it is each treatment be put into foster worm incubator, when 24,48,72 h after check treatment while and control while borer population, calculate
Scattering ratio.
The grade scale of scattering ratio：
0 grade：Scattering ratio is 0；III grade：Scattering ratio is 40.1~60%
I grade：Scattering ratio is 0.1~20%；IV grade：Scattering ratio is 60.1~80%
II grade：Scattering ratio is 20.1~40%；V grade：Scattering ratio is 80.1~100%
The narrow leaved tea tree essential oil of table 1 is to sitophilus zea-mais and the repellent action of confused flour beetle
As shown in Table 1：The present invention is respectively provided with significant repellent action to confused flour beetle and corundum Sitophilusspp. imago, and dosage is got over
Height, its repellent action is bigger；With maximum dose (1.57 μ L/cm2) treatment for examination adult, to the average of confused flour beetle and sitophilus zea-mais
Scattering ratio is respectively 77.60%, 75.68%, and its repellent grade is IV, illustrate the present invention to the repellent effect of confused flour beetle compared with
Higher than sitophilus zea-mais.Under same dose, with the extension of process time, because the volatilization of liquid causes concentration to decline so that drive
The effect of keeping away is overall to be presented downward trend.
Embodiment 2：Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais action of contace poison
1st, for examination insect
It is identical with embodiment 1 for examination insect confused flour beetle, sitophilus zea-mais.
2nd, for examination narrow leaved tea tree essential oil：
It is identical with embodiment 1 for examination essential oil
3rd, test method
Using filter paper medicine embrane method.The filter paper of diameter 9cm is put into the culture dish of same diameter, internal diameter is placed on filter paper
8cm, the glass ring that 5cm inwalls high scribble polytetrafluoroethylene (PTFE) prevents test worm from swashing.Essential oil is diluted to various concentrations with acetone
Liquid, and pipette the liquid 1mL of each concentration respectively with liquid-transfering gun, to instill and be completely absorbed liquid on filter paper, treat that acetone is complete
After volatilization, by 30 access test worms of every ware, feed is not added with ware, covers wire netting, prevent test worm from escaping.Make empty with 1mL acetone
White control, often treatment is repeated 3 times.Each treatment is put into foster worm incubator, it is dead respectively at treatment 24,48,72h investigation test worm
Number.Test worm is touched without any reactor as death standard with writing brush.The death rate is calculated, and uses Abbott formula corrections.
Action of contace poison of the narrow leaved tea tree essential oil of table 2 to sitophilus zea-mais and confused flour beetle
Narrow leaved tea tree essential oil all has obvious action of contace poison to confused flour beetle and sitophilus zea-mais as can be known from Table 2.Treatment
After 24h, concentration is 6.29 μ L/cm2When, the death rate of confused flour beetle and sitophilus zea-mais respectively reaches 98.89%, 97.78%, tags
Effect is significantly increased with the increase for the treatment of dosage；Under same treatment dosage, and its contact efficacy is with the extension of time
And gradually strengthen；After treatment 48h, action of contace poison of the narrow leaved tea tree essential oil to confused flour beetle and sitophilus zea-mais reaches 100%.
Embodiment 3：Narrow leaved tea tree essential oil is to grain storage pest confused flour beetle, the application of sitophilus zea-mais fumigation action
1st, for examination insect
It is identical with embodiment 1 for examination insect confused flour beetle, sitophilus zea-mais.
2nd, for examination narrow leaved tea tree essential oil：
It is identical with embodiment 1 for examination essential oil
3rd, test method
Using wide-mouth bottle filter paper fumigating system.Test worm 30 is only put into the wide-mouth bottle that volume is 300mL, bottle inwall is coated poly-
Tetrafluoroethene.The essential oil for drawing various concentrations respectively with liquid-transfering gun is dripped on 2 × 3cm filter paper bars, rapidly hangs on filter paper bar
In conical flask, filter paper bar can not be contacted with bottle wall, be stoppered bottle stopper sealing.It is placed in after treatment in 30 DEG C of constant incubators and is cultivated,
And to be not added with any reagent as blank.24th, 48, observed after 72h and record test worm death condition in each bottle, calculate dead
Rate and use Abbott formula corrections.Each treatment is repeated 3 times.
Fumigation action of the narrow leaved tea tree essential oil of table 3 to sitophilus zea-mais and confused flour beetle
Narrow leaved tea tree essential oil all has obviously fumigation action to confused flour beetle and sitophilus zea-mais as can be known from Table 3.
After treatment 72h, when concentration is 13.33 μ L/L, the death rate of confused flour beetle and sitophilus zea-mais respectively reaches 98.86%, 92.05%,
Fumigating effect is significantly increased with the increase for the treatment of dosage；Under same treatment dosage, and its fumigating effect is with the time
Extend and significantly increase.
Claims (2)
1. application of the narrow leaved tea tree essential oil to grain storage pest confused flour beetle action of contace poison, narrow leaved tea tree essential oil main component matter
Measuring percentage composition is：4- terpinols 40.09%, γ-terpinene 21.85%, α-terpinene 11.34%, alpha-terpineol 6.91%,
Australene 5.86%, terpinolene 3.24%, the, limonenes 1.36% of 1,8- cineoles 1.83%, p-cymene 1.2%, sabinene
0.2%.
2. the application according to the narrow leaved tea tree essential oil of claim 1 to grain storage pest confused flour beetle action of contace poison, its feature exists
In narrow leaved tea tree essential oil consumption is 4.72-6.29 μ L/cm2, confused flour beetle is continuous raising 5 more than generation.
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| CN110651797A (en) * | 2018-06-28 | 2020-01-07 | 云南森美达生物科技有限公司 | Plant extract, compound botanical insecticide, preparation method and application |
| CN109479904B (en) * | 2018-12-18 | 2021-05-14 | 安徽农业大学 | Application of taxus chinensis essential oil in agricultural pest control |
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