CN107343503A - The application of terpenoid and apply its product - Google Patents
The application of terpenoid and apply its product Download PDFInfo
- Publication number
- CN107343503A CN107343503A CN201710606206.2A CN201710606206A CN107343503A CN 107343503 A CN107343503 A CN 107343503A CN 201710606206 A CN201710606206 A CN 201710606206A CN 107343503 A CN107343503 A CN 107343503A
- Authority
- CN
- China
- Prior art keywords
- terpenoid
- octanols
- concentration
- dimethyl
- seat chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention provides a kind of application of terpenoid and its product is applied, is related to technical field of plant protection, the present invention is experimentally confirmed, and terpenoid can effectively suppress the growth and breeding of grape seat chamber bacterium.Therefore, the product for suppressing grape seat chamber bacterium is prepared using terpenoid, effective inhibitory action can be reached to grape seat chamber bacterium.Medicine provided by the present invention for suppressing grape seat chamber bacterium, including terpenoid, there is good antimicrobial effect, easily decompose, nonhazardous, catabolite will not produce the advantages of endangering to Man ＆ Nature.Provided by the present invention for preventing and/or administering by the agricultural chemicals of the microbial Tree resistance of grape seat chamber, including terpenoid, with prevention effect is good, nontoxic labile advantage, traditional chemical pesticide can effectively be substituted, preventing and treating to canker is significant, have broad application prospects.
Description
Technical field
The present invention relates to technical field of plant protection, a kind of application more particularly, to terpenoid and its production is applied
Product.
Background technology
Terpenoid is the metabolite of plant, is one of main component of plant volatile oil (also known as essential oil), energy
The volatility and the grease of fragrance extracted from the flower of plant, fruit, leaf, stem, root, with the shape of terpenes and containing oxygen derivative
Formula is widely present in nature, and its containing oxygen derivative is mainly that the forms such as alcohol, aldehyde, ketone, carboxylic acid, ester and glycosides are present.Terpene
Compound has compared with strong biological activity, has desinsection, expelling parasite and bioactivity, the content such as antibacterial higher for composite family, Lauraceae, pine
The plants such as section, the rose family.Bacteriostatic activity mechanism is that terpenoid is destroyed to the Subfilament Structure in mycelial cell, so as to broken
The motion of bad cell and intercellular matter transportation, suppress its growth and breeding；And membrane structure is destroyed, cause cell
Osmosis imbalance.
Botryosphaeria (Botryosphaeria) fungi is worldwide widely distributed, is disease common on fruit tree
Opportunistic pathogen, endophyte and saprophytic bacteria, the symptoms such as fruit rot, tikka, necrosis, ulcer and gummosis can be caused.B.dothidea is Portugal
One of most common species of grape seat chamber Pseudomonas, endangers fruit trees such as grape, apple, Kiwi berrys.After fruit is infected, initial stage table
Now it is recessed for water stain shape, scab is in concentric wheel stripe sometimes, with the aggravation of fruit rot and the rupture of epidermis, is produced on fruit big
White hypha is measured, then shrivelled, surface is with many black pycnidias.Meanwhile research shows that grape seat chamber bacterium also endangers
A variety of big specification color leaves view and admire arbor, and caused canker is one of important disease, cause the fragrant tree of L. styraciflua, lotus, North America red
The seeds such as oak, willow are dead serious.
Mainly have to the prevention and controls of canker at present based on chemical prevention, physical control, biological control and management preventing and treating.
Physical control is often cured the symptoms, not the disease, and does not reach effectively preventing effect.Chemical agent disease control after fruit picking is prevented and treated
In play an important role, but it brings many problems, such as：Environment, food security, drug-fast generation etc..People are to life
The raising of quality, and the raising of awareness of safety, develop efficient non-harmful sub and have become the task of top priority.
Therefore, develop that a kind of effect is good, easy decomposition, nonhazardous, catabolite will not produce harm to Man ＆ Nature, can
Agricultural chemicals for preventing and treating canker is particularly important.
In view of this, it is special to propose the present invention.
The content of the invention
First purpose of the present invention is being prepared for suppressing in the product of grape seat chamber bacterium in offer terpenoid
Application, second object of the present invention is to provide a kind of medicine for being used to suppress grape seat chamber bacterium, the of the invention the 3rd
Purpose is preparing the application for preventing and/or administering in the product of Tree resistance, the present invention in offer terpenoid
The 4th purpose be to provide a kind of agricultural chemicals for being used to preventing and/or administering Tree resistance, deposited in the prior art with alleviating
Preventing and treating canker effect it is poor, or agricultural chemicals catabolite to Man ＆ Nature produce harm technical problem.
The invention provides terpenoid to prepare the application in being used to suppress the product of grape seat chamber bacterium.
Further, the terpenoid is included in 3,7- dimethyl -1- octanols, linalool or geraniol acetate
It is one or more of.
Further, the product is medicine.
Present invention also offers a kind of medicine for being used to suppress grape seat chamber bacterium, the medicine includes terpenoid.
Further, the terpenoid includes the material in following (a) or (b)：
(a) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the virtue that concentration is 0.025-0.2 mg/mL
Camphor tree alcohol, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and linalool is 1:2-2:1；
(b) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the perfume (or spice) that concentration is 0.025-0.2 mg/mL
Thatch alcohol acetic ester, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1.
Present invention also offers terpenoid to prepare for preventing and/or administering answering in the product of Tree resistance
With the canker is microbial by grape seat chamber.
Further, the terpenoid is included in 3,7- dimethyl -1- octanols, linalool or geraniol acetate
It is one or more of.
Further, the product is agricultural chemicals.
In addition, present invention also offers a kind of agricultural chemicals for being used to preventing and/or administering Tree resistance, the agricultural chemicals includes
Terpenoid.
Further, the terpenoid includes the material in following (a) or (b)：
(a) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the virtue that concentration is 0.025-0.2 mg/mL
Camphor tree alcohol, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and linalool is 1:2-2:1；
(b) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the perfume (or spice) that concentration is 0.025-0.2 mg/mL
Thatch alcohol acetic ester, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1.
It is demonstrated experimentally that terpenoid can effectively suppress the growth and breeding of grape seat chamber bacterium.Therefore, using terpene
Compound prepares the product for suppressing grape seat chamber bacterium, can reach effective inhibitory action to grape seat chamber bacterium.The present invention carries
What is supplied is used to suppress the medicine of grape seat chamber bacterium, including terpenoid, has good antimicrobial effect, easily decomposes, nonhazardous, decomposes
Product will not produce the advantages of endangering to Man ＆ Nature.Drawn provided by the present invention for preventing and/or administering by grape seat chamber bacterium
The agricultural chemicals of the Tree resistance risen, including terpenoid have that prevention effect is good, nontoxic labile advantage, can be effective
Traditional chemical pesticide is substituted, the preventing and treating to canker is significant, have broad application prospects.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is part of the embodiment of the present invention to apply example, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
The invention provides terpenoid to prepare the application in being used to suppress the product of grape seat chamber bacterium.
In the present invention, terpenoid is monoterpenes compound.
In one preferred embodiment, terpenoid includes 3,7- dimethyl -1- octanols, linalool or citronellol
One or more in acetic acid esters.
In the present invention, product is medicine.
Present invention also offers a kind of medicine for being used to suppress grape seat chamber bacterium, including terpenoid.
In the present invention, above-mentioned terpenoid includes the material in following (a) or (b)：
(a) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the virtue that concentration is 0.025-0.2 mg/mL
Camphor tree alcohol, wherein, the mass ratio of 3,7- dimethyl -1- octanols and linalool is 1:2-2:1；
Wherein, the concentration of 3,7- dimethyl -1- octanols for example can be, but be not limited to 0.025mg/mL, 0.05mg/mL,
0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The concentration of linalool for example can be, but be not limited to 0.025mg/mL,
0.05mg/mL, 0.1mg/mL, 0.15mg/mL or 0.2 mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and linalool is such as
It can be, but be not limited to 1:2、 1:1.5、1:1、1.5:1 or 2:1；
In one preferred embodiment, the concentration of 3,7- dimethyl -1- octanols is 0.1mg/mL；The concentration of linalool
For 0.1mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and linalool is 1:1 or 2:1.
(b) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the perfume (or spice) that concentration is 0.025-0.2 mg/mL
Thatch alcohol acetic ester, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1；
Wherein, the concentration of 3,7- dimethyl -1- octanols for example can be, but be not limited to 0.025mg/mL, 0.05mg/mL,
0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The concentration of geraniol acetate for example can be, but be not limited to 0.025mg/mL,
0.05mg/mL, 0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The quality of 3,7- dimethyl -1- octanols and geraniol acetate
Ratio such as can be, but be not limited to 1:2、1:1.5、1:1、1.5:1 or 2:1；
In one preferred embodiment, the concentration of 3,7- dimethyl -1- octanols is 0.1mg/mL；Geraniol acetate
Concentration be 0.1mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and geraniol acetate is 1:1.
Present invention also offers terpenoid to prepare for preventing and/or administering answering in the product of Tree resistance
With, wherein, canker is microbial by grape seat chamber.
In the present invention, terpenoid is monoterpenes compound.
In one preferred embodiment, terpenoid includes 3,7- dimethyl -1- octanols or linalool, citronellol
One or more in acetic acid esters.
In the present invention, product is agricultural chemicals.
Present invention also offers a kind of agricultural chemicals for being used to preventing and/or administering Tree resistance, including terpenoid.
In the present invention, above-mentioned terpenoid includes the material in following (a) or (b)：
(a) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the virtue that concentration is 0.025-0.2 mg/mL
Camphor tree alcohol, wherein, the mass ratio of 3,7- dimethyl -1- octanols and linalool is 1:2-2:1；
Wherein, the concentration of 3,7- dimethyl -1- octanols for example can be, but be not limited to 0.025mg/mL, 0.05mg/mL,
0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The concentration of linalool for example can be, but be not limited to 0.025mg/mL,
0.05mg/mL, 0.1mg/mL, 0.15mg/mL or 0.2 mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and linalool is such as
It can be, but be not limited to 1:2、 1:1.5、1:1、1.5:1 or 2:1；
In one preferred embodiment, the concentration of 3,7- dimethyl -1- octanols is 0.1mg/mL；The concentration of linalool
For 0.1mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and linalool is 1:1 or 2:1.
(b) the 3,7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the perfume (or spice) that concentration is 0.025-0.2 mg/mL
Thatch alcohol acetic ester, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1；
Wherein, the concentration of 3,7- dimethyl -1- octanols for example can be, but be not limited to 0.025mg/mL, 0.05mg/mL,
0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The concentration of geraniol acetate for example can be, but be not limited to 0.025mg/mL,
0.05mg/mL, 0.1mg/mL, 0.15mg/mL or 0.2mg/mL；The quality of 3,7- dimethyl -1- octanols and geraniol acetate
Ratio such as can be, but be not limited to 1:2、1:1.5、1:1、1.5:1 or 2:1；
In one preferred embodiment, the concentration of 3,7- dimethyl -1- octanols is 0.1mg/mL；Geraniol acetate
Concentration be 0.1mg/mL；The mass ratio of 3,7- dimethyl -1- octanols and geraniol acetate is 1:1.
The present inventor is experimentally confirmed, and terpenoid can effectively suppress the growth of grape seat chamber bacterium and numerous
Grow.Therefore, the product for suppressing grape seat chamber bacterium is prepared using terpenoid, can reaches effective to grape seat chamber bacterium
Inhibitory action.Medicine provided by the present invention for suppressing grape seat chamber bacterium, from plant, there is good antimicrobial effect, easily divide
Solution, nonhazardous, catabolite will not produce the advantages of endangering to Man ＆ Nature.Provided by the present invention for preventing and/or administering
By the agricultural chemicals of the microbial Tree resistance of grape seat chamber, have that prevention effect is good, nontoxic labile advantage, be typically to plant
Material resource agricultural chemicals, traditional chemical pesticide can be effectively substituted, the preventing and treating to canker is significant, there is wide application
Prospect.
In order to help to be better understood from the present invention, now it is described in detail as follows by specific embodiment.
Inhibitory action of the 1 different terpenoids of embodiment to grape seat chamber bacterium
Grape seat chamber bacterium (Botryosphaeria dothidea) isolates and purifies gained from plant diseased plant, utilizes routine
Tissue isolation isolates and purifies pathogen, and observes its morphological feature, and combines rDNA-ITS sequence analyses, to cause of disease
The germ that bacterium isolated and purified obtained by separation and Molecular Identification, which is placed in medical refrigerated refrigerator, to be preserved, is standby.
Using the bacteriostatic activity of mycelial growth rate method measure terpenoid.Grape seat chamber bacterium is trained in PDA culture medium
After supporting 5 days, condition of culture is 28 ± 2 DEG C.By 12mg (±)-terpenes -4- alcohol standard items, 12mg linalools standard items, 12mg 3,
7- dimethyl -1- octanols standard items, 12mg dihydro linalools standard items, 12mg camphors standard items, 12mg (±)-citronellal standard
Product, 12mg geraniol acetates standard items, 12mg bergamios standard items, 12mg (-)-fenchone standard items and 12mg (-)-
Verbenone standard items are added separately in 30mL PDA culture mediums, and being poured into after shaking up in culture dish makes to contain in its culture medium
The concentration of terpenoid reaches 0.4mg/mL (referring to standard items weight content), after culture medium cooling, aseptically, uses
6mm card punch takes growing way identical bacteria cake in cultured beaten for examination bacterium colony edge, accesses among different culture dishes, profit
By the use of blank solvent (i.e. methanol) as compareing, per three repetitions of ware, it is positioned in biochemical cultivation case and cultivates, condition of culture 28 ± 2
℃.After five days, hyphal diameter is measured with crossing method, calculates its bacteriostasis rate.
Computational methods are：Bacteriostasis rate=(control colony diameter-bacteria cake diameter)-(processing colony diameter-bacteria cake diameter)/
Compare colony diameter-bacteria cake diameter.
As a result show：Repetition test using terpenoid to pathogen, it is determined that sterilization, fungistatic effect (is shown in Table
1).Found by the experiment to a variety of terpenoids, concentration is 0.4mg/mL 3,7- dimethyl -1- octanols and concentration is
The fungistatic effect of 0.4mg/mL linalools is best.
Inhibitory action of the 1 different terpenoids of table to grape seat chamber bacterium disease germ
Note：Different letters represent significant difference (P be present in table<0.05)
Inhibitory action of the 2 two kinds of terpenoid compositions same ratios of embodiment to grape seat chamber bacterium
By two kinds of different terpenoids according to mass ratio 1:After 1 is mixed into mixture, by 6mg, 3mg, 1.5mg and
0.75mg terpenoid compositions not of the same race are added in 30mL PDA culture mediums, and being poured into after shaking up in culture dish makes its culture
The concentration containing medicine reaches 0.2mg/mL in base, 0.1 mg/mL, 0.05mg/mL and 0.025mg/mL (refers to drug weight to contain
Amount), specific bacteriostatic test operating method and data processing method are identical with the method provided in experimental example 1, using being not added with terpene
The culture dish of compound is as blank control.
Computational methods are：Bacteriostasis rate=(control colony diameter-bacteria cake diameter)-(processing colony diameter-bacteria cake diameter)/
Compare colony diameter-bacteria cake diameter.
As a result show：Repetition test using terpenoid compositions to pathogen, it is determined that sterilization, fungistatic effect
(being shown in Table 2).Found by the experiment to a variety of terpenoid compositions, linalool and 3,7- dimethyl -1- octanols and 3,
7- dimethyl -1- octanols and geraniol acetate are respectively 1 according to mass ratio:The fungistatic effect of 1 mixing is higher than other terpenes
Polymer composition.
2 two kinds of terpenoids (1 of table:1) fungistatic effect of the mixing to grape seat chamber bacterium disease germ
Note：Different letters represent significant difference (P be present in table<0.05)
Inhibitory action of the 3 two kinds of terpenoid compositions different proportions of embodiment to grape seat chamber bacterium
By two kinds of different terpenoids according to mass ratio 1:2 or 2:After 1 is mixed into mixture, by 6mg, 3mg, 1.5mg
It is added to 0.75mg medicines in 30mL PDA culture mediums, being poured into after shaking up in culture dish makes in its culture medium containing the dense of medicine
Degree reaches 0.2mg/mL, 0.1mg/mL, 0.05 mg/mL and 0.025mg/mL (referring to drug weight content), specific bacteriostatic test behaviour
It is identical with the method provided in experimental example 1 to make method and data processing method, by the use of be not added with the culture dish of terpenoid as
Blank control.
Computational methods are：Bacteriostasis rate=(control colony diameter-bacteria cake diameter)-(processing colony diameter-bacteria cake diameter)/
Compare colony diameter-bacteria cake diameter.
As a result show：Repetition test using terpenoid compositions to pathogen, it is determined that sterilization, fungistatic effect
(being shown in Table 3).Find that 3,7- dimethyl -1- octanols and linalool are according to matter by the experiment to a variety of terpenoid compositions
Amount is than being 2:The fungistatic effect of 1 mixing is significantly higher than other terpenoid compositions.
Inhibitory action of the 3 two kinds of terpenoid compositions different proportions of table to grape seat chamber bacterium disease germ
Effect example terpenoid compositions different ratio bacteriostasis rate is evaluated
For terpenoid linalool, 3,7- dimethyl -1- octanols and geraniol acetate, according to the embodiment of the present invention
The 1 bacteriostatic test operating method and data processing method provided, is evaluated the inhibiting rate of grape seat chamber bacterium, in specific experiment
Hold and result is as follows：
Experimental group 1-1：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the linalool that concentration is 0.1mg/mL
It is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 1-2：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.025mg/mL are 0.2 mg/mL
Alcohol is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 1-3：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.2mg/mL are 0.025 mg/mL
Alcohol is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 2-1：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the linalool that concentration is 0.1mg/mL
It is 1 according to mass ratio:2 carry out being mixed to get composition.
Experimental group 2-2：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.025mg/mL are 0.2 mg/mL
Alcohol is 1 according to mass ratio:2 carry out being mixed to get composition.
Experimental group 2-3：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.2mg/mL are 0.025 mg/mL
Alcohol is 1 according to mass ratio:2 carry out being mixed to get composition.
Experimental group 3-1：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the linalool that concentration is 0.1mg/mL
It is 2 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 3-2：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.025mg/mL are 0.2 mg/mL
Alcohol is 2 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 3-3：The fragrant camphor tree that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.2mg/mL are 0.025 mg/mL
Alcohol is 2 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 4-1：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the citronellol that concentration is 0.1mg/mL
Acetic acid esters is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 4-2：3,7- dimethyl -1- the octanols that concentration is 0.025mg/mL and the lemongrass that concentration is 0.2 mg/mL
Alcohol acetic ester is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 4-3：3,7- dimethyl -1- the octanols that concentration is 0.2mg/mL and the lemongrass that concentration is 0.025 mg/mL
Alcohol acetic ester is 1 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 5：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the linalool that concentration is 0.1mg/mL by
It is 1 according to mass ratio:3 carry out being mixed to get composition.
Experimental group 6：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the linalool that concentration is 0.1mg/mL by
It is 3 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 7：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the citronellol second that concentration is 0.1mg/mL
Acid esters is 3 according to mass ratio:1 carries out being mixed to get composition.
Experimental group 8：3,7- dimethyl -1- the octanols that concentration is 0.1mg/mL and the citronellol second that concentration is 0.1mg/mL
Acid esters is 1 according to mass ratio:3 carry out being mixed to get composition.
Computational methods are：Bacteriostasis rate=(control colony diameter-bacteria cake diameter)-(processing colony diameter-bacteria cake diameter)/
Compare colony diameter-bacteria cake diameter.As a result it is as shown in the table.
| Group | Bacteriostasis rate (%) |
| Experimental group 1-1 | 64.53 |
| Experimental group 1-2 | 61.27 |
| Experimental group 1-3 | 62.02 |
| Experimental group 2-1 | 62.30 |
| Experimental group 2-2 | 61.44 |
| Experimental group 2-3 | 61.89 |
| Experimental group 3-1 | 65.80 |
| Experimental group 3-2 | 64.18 |
| Experimental group 3-3 | 63.94 |
| Experimental group 4-1 | 64.21 |
| Experimental group 4-2 | 64.05 |
| Experimental group 4-3 | 63.51 |
| Experimental group 5 | 30.46 |
| Experimental group 6 | 24.68 |
| Experimental group 7 | 22.19 |
| Experimental group 8 | 17.55 |
Terpenoid compositions that application experiment group 1-1 to 4-3 provides are can be seen that to grape seat from the result of upper table
For the bacteriostasis rate of chamber bacterium more than 60%, fungistatic effect is higher than the terpenoid compositions that experimental group 5 to 8 provides, and illustrates this
The terpenoid compositions provided are invented by the coordinated between specific component and proportioning, grape seat chamber bacterium can be reached
To more efficient inhibitory action, the preventing and treating to canker is significant, has broad application prospects.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. terpenoid is preparing the application in being used to suppress the product of grape seat chamber bacterium.
2. application according to claim 1, it is characterised in that the terpenoid include 3,7- dimethyl -1- octanols,
One or more in linalool or geraniol acetate.
3. application according to claim 1 or 2, it is characterised in that the product is medicine.
4. a kind of medicine for being used to suppress grape seat chamber bacterium, it is characterised in that the medicine includes terpenoid.
5. medicine according to claim 4, it is characterised in that the terpenoid includes the thing in following (a) or (b)
Matter：
(a) 3, the 7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the linalool that concentration is 0.025-0.2mg/mL,
Wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and linalool is 1:2-2:1；
(b) citronellol that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.025-0.2mg/mL are 0.025-0.2mg/mL
Acetic acid esters, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1.
6. terpenoid is preparing the application for preventing and/or administering in the product of Tree resistance, it is characterised in that institute
It is microbial by grape seat chamber to state canker.
7. application according to claim 6, it is characterised in that the terpenoid include 3,7- dimethyl -1- octanols,
One or more in linalool or geraniol acetate.
8. the application according to claim 6 or 7, it is characterised in that the product is agricultural chemicals.
9. a kind of agricultural chemicals for being used to preventing and/or administering Tree resistance, it is characterised in that the agricultural chemicals includes terpenoid
Thing.
10. agricultural chemicals according to claim 9, it is characterised in that the terpenoid is included in following (a) or (b)
Material：
(a) 3, the 7- dimethyl -1- octanols that concentration is 0.025-0.2mg/mL and the linalool that concentration is 0.025-0.2mg/mL,
Wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and linalool is 1:2-2:1；
(b) citronellol that the 3,7- dimethyl -1- octanols and concentration that concentration is 0.025-0.2mg/mL are 0.025-0.2mg/mL
Acetic acid esters, wherein, the mass ratio of 3, the 7- dimethyl -1- octanols and geraniol acetate is 1:2-2:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710606206.2A CN107343503A (en) | 2017-07-24 | 2017-07-24 | The application of terpenoid and apply its product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710606206.2A CN107343503A (en) | 2017-07-24 | 2017-07-24 | The application of terpenoid and apply its product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107343503A true CN107343503A (en) | 2017-11-14 |
Family
ID=60257843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710606206.2A Pending CN107343503A (en) | 2017-07-24 | 2017-07-24 | The application of terpenoid and apply its product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107343503A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371187A (en) * | 2018-01-16 | 2018-08-07 | 浙江大学 | Application of the terpene volatile materials in inhibiting strawberry spherical cavity bacterium |
| CN110959633A (en) * | 2019-10-29 | 2020-04-07 | 湖北工程学院 | Callicarpa longifolia extract and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1592578A (en) * | 2001-10-04 | 2005-03-09 | 以色列国家农业部富卡尼中心 | Microbiocidal formulation comprising essential oils or their derivatives |
-
2017
- 2017-07-24 CN CN201710606206.2A patent/CN107343503A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1592578A (en) * | 2001-10-04 | 2005-03-09 | 以色列国家农业部富卡尼中心 | Microbiocidal formulation comprising essential oils or their derivatives |
Non-Patent Citations (2)
| Title |
|---|
| 李素清等: "几种植物精油对红阳猕猴桃中两种致病菌抑制效果的研究", 《四川农业大学学报》 * |
| 王璠等: "葡萄座腔菌属(Botryosphaeria)引起的果树病害及研究进展", 《植物保护》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371187A (en) * | 2018-01-16 | 2018-08-07 | 浙江大学 | Application of the terpene volatile materials in inhibiting strawberry spherical cavity bacterium |
| CN108371187B (en) * | 2018-01-16 | 2019-09-10 | 浙江大学 | Terpene volatile materials is inhibiting the application in strawberry spherical cavity bacterium |
| CN110959633A (en) * | 2019-10-29 | 2020-04-07 | 湖北工程学院 | Callicarpa longifolia extract and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hashem et al. | Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp. | |
| Mackeen et al. | Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ulam) | |
| Li et al. | Antifungal effects of citronella oil against Aspergillus niger ATCC 16404 | |
| Moleyar et al. | Antifungal activity of some essential oil components | |
| Okigbo et al. | Antifungal effects of two tropical plant leaf extracts (Ocimum gratissimum and Aframomum melegueta) on postharvest yam (Dioscorea spp.) rot | |
| Amadi et al. | Isolation and identification of fungi involved in the post-harvest spoilage of guava (Psidium guajava) in Awka metropolis | |
| Behbahani et al. | Antifungal effect of aqueous and ethanolic mangrove plant extract on pathogenic fungus" in vitro". | |
| Cavanagh | Antifungal activity of the volatile phase of essential oils: a brief review | |
| CN105494460B (en) | Application of the narrow leaved tea tree essential oil on preventing and treating grain storage pest | |
| Jeff-Agboola et al. | In vitro antifungal activities of essential oil from Nigerian medicinal plants against toxigenic Aspergillus flavus | |
| US20110136758A1 (en) | Carbohydrate compositions from basidiomycete fungi as biocidal agents active against pathogens | |
| Zhang et al. | Nano-emulsification essential oil of Monarda didyma L. to improve its preservation effect on postharvest blueberry | |
| CN107343503A (en) | The application of terpenoid and apply its product | |
| Hassan et al. | Assessing the use of aloe vera gel alone and in combination with lemongrass essential oil as a coating material for strawberry fruits: HPLC and EDX analyses | |
| Hadian et al. | Antifungal potency of some essential oils in control of postharvest decay of strawberry caused by Botrytis cinerea, Rhizopus stolonifer and Aspergillus niger | |
| CN107955796B (en) | Red silk ribbon attached to an official seal or a medal aspergillus Q-1 and its application in termite control | |
| Hesami et al. | Effect of Pistacia atlantica subsp. kurdica essential oil and acetic acid on Botrytis cinerea growth in culture media and strawberry fruits | |
| CN104800134A (en) | Preservative for skin care product | |
| CN110527639A (en) | A kind of U.S. pole plum surprise yeast and its application | |
| Obiazikwor et al. | Antifungal activity of silver nanoparticles synthesized using Citrus sinensis peel extract against fungal phytopathogens isolated from diseased tomato (Solanum lycopersicum L.) | |
| CN110742076B (en) | Application of liquiritin in preventing and treating crop oomycetes and fungal diseases | |
| CN103548833B (en) | Acaricidal composition containing pyriminostrobin and propargite | |
| CN103018409A (en) | Method for testing bacteriostatic effect of wild cactus polysaccharide | |
| Abdulsalam et al. | Isolation and control of fungi associated with neck rot disease of onions (Allium Cepa L.) in Bama, Borno State, Nigeria | |
| Diba et al. | Antifungal activity of Satureja hortensis alcoholic extract against Aspergillus and Candida species |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171114 |
|
| RJ01 | Rejection of invention patent application after publication |