CN110959633A - Callicarpa longifolia extract and preparation method and application thereof - Google Patents
Callicarpa longifolia extract and preparation method and application thereof Download PDFInfo
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- CN110959633A CN110959633A CN201911038366.7A CN201911038366A CN110959633A CN 110959633 A CN110959633 A CN 110959633A CN 201911038366 A CN201911038366 A CN 201911038366A CN 110959633 A CN110959633 A CN 110959633A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The first purpose of the invention is to provide a preparation method of a beautyberry leaf extract, which comprises the following steps: pulverizing Callicarpa longifolia, mixing with volatile organic solvent, treating with ultrasonic wave, leaching, filtering, and concentrating to obtain Callicarpa longifolia extract. The second purpose of the invention is to provide a callicarpa longifolia extract, the third purpose of the invention is to provide a drug for inhibiting staphylococcus, and the fourth purpose of the invention is to provide the application of the drug for inhibiting staphylococcus in preparing products for preventing and/or treating tree canker, so as to relieve the technical problems of poor effect of preventing and treating canker or harm of pesticide residue and decomposition products to human bodies and nature in the prior art.
Description
Technical Field
The invention belongs to the technical field of plant-derived bacteriostatic agents, and particularly relates to a callicarpa longifolia extract and a preparation method and application thereof.
Background
The Botryosphaeria (Botryosphaeria) fungi are widely distributed in the world, most of the fungi harm large-size color-leaf ornamental trees, and the fungi are common pathogenic bacteria, endophytes and saprophytes on fruit trees and cause symptoms of fruit rot, leaf spot, necrosis, ulcer, gummosis and the like. Dothidea belongs to one of the most common species of plasmopara, and harms fruit trees such as grapes, apples and kiwi fruits. After the fruit is infected, the fruit is in a water stain-like depression at the initial stage, the disease spots are sometimes in concentric ring lines, a large amount of white hyphae are generated on the fruit along with the increase of fruit rot and the rupture of epidermis, and then the fruit is shriveled, and a plurality of black conidiophores grow on the surface. Meanwhile, studies have shown that the gluconobacter vinifera also causes death of species of liquidambar styraciflua, lotus nut trees, quercus rubra, poplar, and the like.
Callicarpa longifolia (Callicarpa longifolia) is a plant of the genus Callicarpa, is distributed in Guangdong, Yunnan and other places, grows in the area with the altitude of 1400 meters, and has the functions of mainly dispelling wind, eliminating dampness, promoting blood circulation, stopping bleeding and the like. It can be used for treating rheumatalgia, cough due to wind-cold evil, and hematemesis. Early studies showed that plants of the genus Callicarpa have high bacteriostatic activity against Staphylococcus aureus and Salmonella, and also have strong activity against Candida albicans, Salmonella typhi, and Shigella dysenteriae. And the callicarpa has different degrees of in vitro antibacterial activity, wherein the callicarpa nudiflora has the most extensive antibacterial activity, and has good antibacterial activity on staphylococcus, pseudomonas aeruginosa, escherichia coli, dysentery bacillus, typhoid bacillus and other bacteria.
Cleomis (Cleome gynandra L.) contains annual and upright branch of herbaceous plants. The wide-area distributed species are distributed from Hainan island to the neighborhood of Beijing in China, from Yunnan to Taiwan, may originally produce ancient tropical zone, and are available in global tropical zone and subtropical zone at present. Is a common weed in low-altitude villages, roadside, wasteland or fields and a few areas of medicinal plants Asia and Africa which are occasionally cultivated for vegetables and foods and can also be pickled. The seeds are ground into powder which is similar to mustard and is used for medicine. The herbs are bitter, pungent and slightly toxic.
Chemical control is a main control measure in comprehensive control for diseases and insect pests for a long time, and has the advantages of quick effect, simple method, strong emergency treatment, no regional and seasonal limitation and the like. However, the long-term and large-scale unscientific use of chemical pesticides leads to the increase of drug resistance of plant diseases and insect pests, which leads to the reduction of prevention and treatment effect, and the more serious problems of pollution of agricultural products, air, soil and water areas, endangering the health, safety and ecological environment of people and livestock, and the like. At present, the development of high-efficiency and low-toxicity biological agents is urgently needed to reduce the use amount of chemical pesticides and realize a green and environment-friendly pest control strategy.
Disclosure of Invention
The invention aims to apply the mixture of the callicarpa longifolia extract and the cleome gynandra extract to the prevention and development of the medicament for preventing and treating the ulcer diseases of plants, and provides a new thought and method for green and efficient prevention and treatment of the ulcer diseases.
The first purpose of the invention is to provide a preparation method of beautyberry leaf extract, the second purpose of the invention is to provide a beautyberry leaf extract, the third purpose of the invention is to provide a drug for inhibiting staphylococcus, and the fourth purpose of the invention is to provide the application of the drug for inhibiting staphylococcus in preparing products for preventing and/or treating tree canker, so as to relieve the technical problems of poor effect of preventing and treating canker or harm of pesticide decomposition products to human and nature in the prior art.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a preparation method of Callicarpa longifolia extract comprises the following steps:
pulverizing Callicarpa longifolia, mixing with volatile organic solvent, treating with ultrasonic wave, leaching, filtering, and concentrating to obtain Callicarpa longifolia extract.
On the basis of the technical scheme, the invention can also have the following further specific selection or optimization selection.
Specifically, the weight ratio of the callicarpa longifolia to the volatile organic solvent is 1: 4-5; the volatile organic solvent is methanol or ethanol; the ultrasonic treatment refers to ultrasonic extraction for 10 minutes at the temperature of 40 ℃; the leaching temperature is normal temperature (about 25 ℃) and the leaching time is 72 hours; and the concentration is carried out by adopting a vacuum rotary evaporator. Preferably, the weight ratio of the beautyberry leaf to the volatile organic solvent is 1: 5.
The ultrasonic extraction method is characterized in that ultrasonic waves generate high speed and strong cavitation effect and stirring effect to destroy cells of plants or medicinal materials, so that the solvent permeates into the plant cells to obtain substances in the plant cells. The ultrasonic extraction is carried out at a temperature, time, frequency and the like which are commonly used for extracting plant components, or can be properly adjusted by a person skilled in the art according to actual conditions.
In addition, the invention also provides a callicarpa longifolia extract, which is prepared by adopting the method.
In addition, the invention also provides a medicine for inhibiting the staphylococcus, which comprises the longleaf violet plant extract, or comprises the longleaf violet plant extract and the cleome gynura extract. The Cleomis extract can be prepared by a method similar to the preparation method of the Callicarpa longifolia extract.
Specifically, the mass part ratio of the longleaf violet plant extract to the white cauliflower extract is 1-4: 1-2.
In addition, the invention also provides application of a drug for inhibiting the staphylococcus aureus in preparing a product for preventing and/or treating the tree canker.
Compared with the prior art, the invention has the beneficial effects that: the Callicarpa longifolia extract or the cleome gynandra extract can effectively inhibit the growth and reproduction of the staphylococcus aureus. Therefore, the product for inhibiting the staphylococcus is prepared by the plant extract, and can achieve effective inhibition on the staphylococcus. The medicine for inhibiting the grape-vine lumen bacteria has the advantages of good bacteriostatic effect, easy decomposition, no toxicity and no harm to human and nature caused by decomposition products. The pesticide for preventing and/or treating the tree canker caused by the staphylococcus comprises Callicarpa longifolia extract and cleome gynandra extract, has the advantages of good prevention and treatment effect, no toxicity and easy decomposition, can effectively replace the traditional chemical pesticide, has important significance for prevention and treatment of canker and has wide application prospect.
Detailed Description
For a better understanding of the present invention, reference is made to the following examples which illustrate the invention
In the following examples, the raw material Callicarpa longifolia is collected in Guangdong province in 2018 years, placed at 40 ℃, dried, crushed and refrigerated for later use; the cleome gynura is collected in filial tract city of Hubei province in 2019 years, placed at 40 ℃, dried, crushed and refrigerated for later use.
The botryococcus viniferus is provided for special fruit and vegetable quality safety control key laboratories of Hubei province of Hubei engineering academy. The botanical pesticide mainly utilizes active ingredients in plants including plants and compounds and derivatives synthesized according to active structures to prevent or reduce pests such as diseases and pests, and has the advantages that: strong specificity, no harm to natural enemies, environmental protection and the like. The research adopts that Callicarpa longifolia extract (cleome gynandra extract) or the mixture of the Callicarpa longifolia extract and the Clerodendranthus longifolia extract is added into a PDA culture medium according to different proportions within 0.4-0.01 g/ml (weight content of extract), and the effect of the extract on the disease bacteria is determined by calculating the bacteriostasis rate of the Callicarpa longifolia extract.
Example 1: inhibition effect of callicarpa longifolia methanol extract on staphylococcus lumen
Taking 50g of the crushed Callicarpa longifolia powder, respectively adding 5 times of methanol (99.5 percent, Tianjin Bodi) by weight, extracting for 20 minutes at 40 ℃, then placing at normal temperature (25 ℃) for leaching for 72 hours, filtering, concentrating the extracted leaching liquor to be slurry or paste by using a vacuum rotary evaporator at 40 ℃, dissolving the slurry or paste by using methanol, centrifuging, taking clear liquid, and concentrating the clear liquid under reduced pressure until the mass percentage content of the methanol is lower than 10 percent to obtain extract.
Weighing a certain amount of extract, and dissolving in methanol to obtain an extract methanol solution with 2 times of the volume of the extract. Adding 50ml (or 5ml) of extract methanol solution into a rapidly-cooled (about 50 ℃)100ml PDA culture medium, shaking uniformly, pouring into a culture dish to enable the concentration of the extract in the culture medium to reach 0.1 (or 0.01) g/ml (indicating the weight content of the extract), after the culture medium is cooled, respectively taking C.gloeospriides and A.alternata bacterial cakes with the diameters of 0.6cm into different culture dishes, using a blank solvent (namely methanol) as a control, repeating three times in each dish, placing the culture dishes in a biochemical incubator for culture, wherein the culture conditions are 28 +/-2 ℃, measuring the diameters of hyphae by using a cross method after the control grows full, and calculating the antibacterial rate.
The calculation method comprises the following steps: the inhibition rate is (control colony diameter-cake diameter) - (treated colony diameter-cake diameter)/control colony diameter-cake diameter.
The results show that: the longleaf beautyberry leaf is extracted by adopting methanol, and the repeated test of the extract on pathogenic bacteria is utilized to determine the sterilization and bacteriostasis effects of the longleaf beautyberry leaf (see table 1), which indicates that the extract can be effectively used for developing the bacteriostat of the grape cavity bacteria.
TABLE 1 inhibitory Effect of Callicarpa longifolia methanol extract on Staphylocoris
Example 2: inhibition effect of longleaf beautyberry ethanol extract on staphylococcus lumen
Freeze-drying Callicarpa longifolia powder, pulverizing, weighing 50g, soaking in 400g ethanol (99.5%, first chemical plant of Hubei Xiantao) respectively, extracting at 40 deg.C for 20 min with ultrasonic wave, leaching at room temperature (25 deg.C) for 72 hr, filtering, concentrating the extractive solution to slurry or paste at 40 deg.C with vacuum rotary evaporator, and performing bacteriostatic test with the same operation method and data processing method as those in experiment example 1, and using blank solvent (ethanol) as control. The experimental results are shown in table 2, and the beautyberry extract experiment finds that the beautyberry extract has the bacteriostatic effect on the staphylococcus aureus.
TABLE 2 inhibitory Effect of ethanol extract of Callicarpa longifolia on Staphylocoris
Example 3 inhibition of Staphylocoris by methanol extract of Cleomis
The cleome gynandra is at-60 ℃, is frozen and dried, is crushed, is weighed to 50g, is respectively soaked in 400g of methanol (the boiling range is 30-60 ℃, and Tianjin is constant), is extracted for 10 minutes by ultrasonic at 38 ℃, is placed at normal temperature (25 ℃) for leaching for 72 hours and is filtered, is concentrated into slurry or paste by a vacuum rotary evaporator at 40 ℃, and then the specific operation method and the data processing method of the bacteriostatic test are the same as those in the experimental example 1, and a blank solvent (namely methanol) is used as a reference. The experimental result is shown in table 4, the cleome gynandra methanol extract has an antibacterial effect on the grape lumen bacteria, wherein the antibacterial rate of 0.04g/ml reaches more than 50%, and the result shows that the extract can be effectively used for developing the antibacterial agent of the grape lumen bacteria.
TABLE 3 inhibitory Effect of methanol extract of Cleomis cauliflora on Staphylocosis
Example 4 inhibition of Staphylotrichum by combination of Oldenlandia diffusa extract and Callicarpa longifolia extract
After the white cauliflower and the beautyberry leaf extract were mixed in different proportions according to the amount of 0.04g/ml, the specific operation method and data processing method of the bacteriostasis test were the same as those described in experimental example 1, using a blank solvent (i.e., methanol) as a control. The experimental results are shown in table 4, and the bacteriostatic experimental results of the mixture of the cleome gynura and the callicarpa longifolia methanol extracts show that the bacteriostatic rate reaches over 60 percent, and the effect is greater than that of a single extract.
TABLE 4 inhibitory Effect of mixture of Oldenlandia diffusa and Callicarpa longifolia on Staphylocoris
According to the experimental data, the mixture of the cleome gynandra and the callicarpa longifolia extracts is found to have an inhibiting effect on the grape-seat cavity bacteria, and an antibacterial experiment on the mixture shows that the antibacterial effect of the mixture of the cleome gynandra and the callicarpa longifolia extracts is obviously greater than that of the cleome gynandra and the callicarpa longifolia extracts when the mass ratio of the cleome gynandra to the callicarpa longifolia extracts is (0.04g/ml), and the mass ratio of the callicarpa longifolia extracts to the cleome gynandra extracts is (1-4): 1-2, the bacteriostatic effect is higher than the bacteriostatic rate of 0.05g/ml of the cleome gynandra extract and 0.2g/ml of the beautyberry extract, and the result shows that the bacteriostatic effect of the mixture of the two extracts is obviously better than that of a single extract under the same quantity condition.
According to the above experiments, the mixture of the extract of the cleome gynandra and the extract of the beautyberry has better bacteriostatic effect compared with the extract with single component.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A preparation method of a beautyberry leaf extract is characterized by comprising the following steps:
pulverizing Callicarpa longifolia, mixing with volatile organic solvent, treating with ultrasonic wave, leaching, filtering, and concentrating to obtain Callicarpa longifolia extract.
2. The method for preparing beautyberry leaf extract according to claim 1, wherein the method comprises the following steps: the volatile organic solvent is methanol or ethanol; the weight ratio of the longleaf beautyberry to the volatile organic solvent is 1: 4-5.
3. The method for preparing beautyberry leaf extract according to claim 1, wherein the method comprises the following steps: the ultrasonic treatment refers to ultrasonic extraction at 40 ℃ for 10 minutes.
4. The method for preparing beautyberry leaf extract according to claim 1, wherein the method comprises the following steps: the leaching temperature is normal temperature, and the leaching time is 72 hours.
5. The method for preparing beautyberry leaf extract according to claim 1, wherein the method comprises the following steps: and the concentration is carried out by adopting a vacuum rotary evaporator.
6. An extract of Callicarpa longifolia, characterized in that: which is prepared by the preparation method of the Callicarpa longifolia extract as described in any one of claims 1 to 5.
7. A medicament for inhibiting gluconobacter vinifera is characterized in that: comprising the extract of the longleaf violet plant of claim 6.
8. The agent for inhibiting gluconobacter according to claim 7, wherein: also comprises herba Cleomis extract.
9. The agent for inhibiting gluconobacter according to claim 8, wherein: the mass part ratio of the longleaf violet plant extract to the cleome gynandra extract is 1-4: 1-2.
10. Use of a drug inhibiting gluconobacter according to claims 7-9 in the preparation of a product for the prevention and/or treatment of tree canker.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1915997A1 (en) * | 2005-08-12 | 2008-04-30 | KOHNO, Kenji | Agent for hair growth |
| CN104488977A (en) * | 2015-01-19 | 2015-04-08 | 湖北工程学院 | Chinese ash bark and fruit extractive and novel application thereof |
| CN105078803A (en) * | 2014-05-16 | 2015-11-25 | 苏州润新生物科技有限公司 | Application of callicarpa plant extract |
| CN107343503A (en) * | 2017-07-24 | 2017-11-14 | 湖北工程学院 | The application of terpenoid and apply its product |
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- 2019-10-29 CN CN201911038366.7A patent/CN110959633A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1915997A1 (en) * | 2005-08-12 | 2008-04-30 | KOHNO, Kenji | Agent for hair growth |
| CN105078803A (en) * | 2014-05-16 | 2015-11-25 | 苏州润新生物科技有限公司 | Application of callicarpa plant extract |
| CN104488977A (en) * | 2015-01-19 | 2015-04-08 | 湖北工程学院 | Chinese ash bark and fruit extractive and novel application thereof |
| CN107343503A (en) * | 2017-07-24 | 2017-11-14 | 湖北工程学院 | The application of terpenoid and apply its product |
Non-Patent Citations (2)
| Title |
|---|
| ELIS SUSILAWATI ET AL: ""Activity of Karehau (Callicarpa longifolia Lamk.) Leaves Ethanolic Extract as a Wound Healing"", 《J. PHARM. SCI. & RES》 * |
| 钟鸣等: "《中国壮药学》", 31 May 2016, 广西民族出版社 * |
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Application publication date: 20200407 |