CN102365939A - Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape - Google Patents
Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape Download PDFInfo
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- CN102365939A CN102365939A CN2011104002040A CN201110400204A CN102365939A CN 102365939 A CN102365939 A CN 102365939A CN 2011104002040 A CN2011104002040 A CN 2011104002040A CN 201110400204 A CN201110400204 A CN 201110400204A CN 102365939 A CN102365939 A CN 102365939A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 235000009754 Vitis X bourquina Nutrition 0.000 title claims abstract description 27
- 235000012333 Vitis X labruscana Nutrition 0.000 title claims abstract description 27
- 235000014787 Vitis vinifera Nutrition 0.000 title claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 title abstract 7
- 238000004659 sterilization and disinfection Methods 0.000 title abstract 7
- 240000006365 Vitis vinifera Species 0.000 title 1
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims abstract description 52
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 241000196324 Embryophyta Species 0.000 claims abstract description 38
- 239000005844 Thymol Substances 0.000 claims abstract description 26
- 241000219095 Vitis Species 0.000 claims abstract description 26
- 229960000790 thymol Drugs 0.000 claims abstract description 26
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000012010 growth Effects 0.000 claims abstract description 4
- 230000001737 promoting effect Effects 0.000 claims abstract description 3
- 230000003115 biocidal effect Effects 0.000 claims description 34
- 239000003139 biocide Substances 0.000 claims description 33
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 19
- -1 polyoxyethylene Polymers 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 7
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- 150000008107 benzenesulfonic acids Chemical class 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 3
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 3
- 241001272567 Hominoidea Species 0.000 claims description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 3
- 239000004359 castor oil Substances 0.000 claims description 3
- 235000019438 castor oil Nutrition 0.000 claims description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229920001568 phenolic resin Polymers 0.000 claims description 3
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 3
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 3
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims description 3
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 claims description 3
- AYAUBWSUZRFVQO-UHFFFAOYSA-N 2-[2-(4-phenyl-5-sulfanylidene-1h-1,2,4-triazol-3-yl)ethyl]benzo[de]isoquinoline-1,3-dione Chemical compound O=C1C(C=23)=CC=CC3=CC=CC=2C(=O)N1CCC1=NNC(=S)N1C1=CC=CC=C1 AYAUBWSUZRFVQO-UHFFFAOYSA-N 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000000749 insecticidal effect Effects 0.000 abstract description 2
- 241000238631 Hexapoda Species 0.000 abstract 1
- 239000000306 component Substances 0.000 abstract 1
- 230000000266 injurious effect Effects 0.000 abstract 1
- 239000005426 pharmaceutical component Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 13
- 230000000694 effects Effects 0.000 description 7
- 241000223259 Trichoderma Species 0.000 description 6
- 239000000575 pesticide Substances 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 5
- 229930002877 anthocyanin Natural products 0.000 description 4
- 235000010208 anthocyanin Nutrition 0.000 description 4
- 239000004410 anthocyanin Substances 0.000 description 4
- 150000004636 anthocyanins Chemical class 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000003449 preventive effect Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 206010039509 Scab Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 239000005839 Tebuconazole Substances 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a botanical sterilization preparation, which contains the following components in percentage by weight: 0.1-50% of thymol, 5-30% of emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water. The invention further discloses a preparation method and application of the botanical sterilization preparation of the botanical sterilization preparation to control of the bitter rot or anthracnose of grape. Pharmaceutical components of the botanical sterilization preparation based on thymol come from plants or extractives thereof with an insecticidal function; the botanical sterilization preparation not only can be used for effectively controlling the bitter rot or anthracnose of grape but also can be used for promoting growth and development of crops, increasing production, increasing income, improving quality, increasing nutrient utilization rate, controlling injurious insects, avoiding pollution and the like; and the botanical sterilization preparation is applied to the production of nuisanceless, green and organic agricultural products.
Description
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of plant resource biocide preparation and the application in preventing and treating bitter rot or anthracnose of grape thereof.
Background technology
Bitter rot or anthracnose of grape (Grape anthracnose) has another name called late blight, is one of important disease during grape is produced.Anthracnose is that Deuteromycotina belongs to, and germ mainly hides at positions such as the annual complicated and confused textura epidermoidea of being infected, leaf scars with mycelium, and is attached to invalid tissues such as deadwood, fallen leaves, decayed fruit with conidium and survives the winter.Generally only occur on the painted or maturescent fruit, but germ can be infected green fruit, climing, leaf and tendril etc., and tissue does not show tangible symptom.Fruit morbidity after painted just produces the circular fleck of the big brown of syringe needle at the fruit face, and spot enlarged gradually afterwards, and depression, grows the pore that wheel line shape is arranged gradually on the surface.When weather was moist, growing pink shot-like particle on the scab was the conidium agglomerate of germ.Fall ill when serious, scab can expand to half or whole fruit face, and the soft corruption of fruit grain be prone to come off, or drying shrinkage becomes mummy gradually.Carpopodium and cob morbidity produce the scab that the crineous Long Circle caves in, and cause fruit to rot in a large number, cause serious loss.
Preventing and treating on the means of bitter rot or anthracnose of grape at present, mainly still chemical control, agricultural chemicals commonly used mainly is mancozeb, tmtd, carbendazim.Efficient, quick-acting, easy to use, economic benefit advantages of higher that bactericide has, but have only protective effect, do not have treatment and root out effect, and the lasting period to be short, application times is many, and germ has produced pesticide resistance to it; Long-time unreasonable use chemical pesticide control bitter rot or anthracnose of grape causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase residue of pesticide in the fruit, and contaminated environment increases production cost.Chemical pesticide still is difficult to solve effectively to people and animals' toxic and side effect and residue problem so far.
Along with the appearance of more and more pesticide resistance phytopathogens, seek that other is alternative, toxic and side effect is little, agricultural chemicals has become the focal issue of agricultural sustainable development safely and effectively.Characteristics such as it is safe, efficient, pollution-free that botanical biological pesticide has require to match with food safety production with preserving the ecological environment.And through the biopesticide of processing after the improvement of vegetable source natural compound, not only control efficiency is good, also to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, no poisoning, advantages such as the non-resistant property of medicine; In the control disease, can effectively improve quality of agricultural product simultaneously.
Summary of the invention
Technical problem to be solved by this invention provides a kind of plant resource biocide preparation based on thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned plant resource biocide preparation based on thymol.
The technical problem that the present invention will solve at last provides the application of above-mentioned plant resource biocide preparation in preventing and treating bitter rot or anthracnose of grape.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%.
Above-mentioned plant resource biocide preparation preferably comprises the component of following percetage by weight: thymol 0.5～5%, and emulsifier 10～20%, azone 2～3%, ethanol 20～40%, water 32～67.5%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Thymol added mix the formation solution A in the part ethanol; Again emulsifier and azone are formed solution B with the residue dissolve with ethanol, then solution A, solution B and water are mixed.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned plant resource biocide preparation in promoting grape growth.
The application of above-mentioned plant resource biocide preparation in preventing and treating bitter rot or anthracnose of grape.
Beneficial effect: its effective component of plant resource biocide preparation based on thymol of the present invention derives from plant or its extract with insecticidal function; Can not only effectively prevent and treat bitter rot or anthracnose of grape; Have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is big, economic benefit and social benefit are obvious, has the wide application prospect of ten minutes.
Embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that embodiment is described only to be used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is a phenethyl phenol polyoxyethylene poly-oxygen propylene aether) 20%; Azone 2%, ethanol 30%, water 46%; And described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/4 amount; Again emulsifier and azone are formed solution B with the dissolve with ethanol that remains 3/4 amount, then solution A, solution B and water are mixed.
Embodiment 2:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 1%, emulsifier (emulsifier is a dialkyl benzene sulfonic acids calcium) 10%, azone 2%, ethanol 20%, water 67%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/2 amount; Again emulsifier and azone are formed solution B with the dissolve with ethanol that remains 1/2 amount, then solution A, solution B and water are mixed.
Embodiment 3:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is phenethyl phenol polyethenoxy ether and APES phosphate by weight 1: 2 mixture) 10%, azone 2%; Ethanol 20%; Water 67.5%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct and aralkyl phenol polyethenoxy ether by weight 1: 1: 1 mixture) 30%, azone 20%; Ethanol 10%; Water 39.9%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium by weight 1: 1: 1 mixture) 5%, azone 0.1%; Ethanol 20%; Water 24.9%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 6: based on the plant resource biocide preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The plant resource biocide preparation of the thymol that formula I I: embodiment 1 makes.
Choose 3 processing of field piece design: handle 1: contrast (contrast CK is a clear water); Handle 2: formula I; Handle 3: formula I I.Each handles repetition 3 times, and treatment fluid is every to spray once at a distance from 5d, sprays continuously 3 times, waits for that gathering in the crops the back sampling analyzes.
Assay method:
The pericarp anthocyanin content: get each and repeat 20 of cold storage fruits, drill through about 30 on fruit grain equatorial plane pericarp with card punch, mixing is weighed; Add 2% hydrochloric acid-ethanol 4mL in the 10mL centrifuge tube, placed 32 ℃ of insulating boxs dippings 2 hours, then take out centrifuge tube and descended 8000 * g centrifugal 10 minutes at 4 ℃; Collect supernatant, add 2% hydrochloric acid-ethanol 3mL lixiviate again, repeat to extract 3 times; Merge supernatant, constant volume is to 10mL.Measure the content of anthocyanin with pH differential methods such as Wang Shaobo.(Wang Shaobo, Du Yongfeng, Yao grasp magnificent .pH differential method and measure the anthocyan in the Testa sojae atricolor. chemical analysis metering, 2008 (1): 46-49)
Fruit juice soluble solid, titratable acid and pH value: break into homogenate with beater after the sample peeling, be divided in 3 centrifuge tubes, 8000 * g is centrifugal 10 minutes under 4 ℃, gets supernatant and is equipped with survey.Soluble solid is measured with the explicit saccharometer of electron number (DR103).Titratable acid content is measured with the acid base neutralization titration method, and is converted into tartaric acid content.The pH value is measured with pH meter.Specifically measure with reference to methods such as Liu Ailings.(what is for army building, Wang Chenghua, Li Weifu, Xie Zhaosen, old Yu, Shen Yuliang, Wang Shiping for Liu Ailing, Lu Zhixing. and nutrient solution concentration is to the influence of " behind the peak " developmental stages of grape berry and quality, southern china fruit tree .2011,40 (4): 74-77.)
Table 1 different disposal is to the influence of grape biology amount and quality
Can know that by table 1 grape is handled back spike number, Dan Suichong, sugar-acid ratio, soluble solid content and pericarp anthocyanin content in formula I and compared respectively according to having increased by 5.6%, 10.3%, 4.1%, 10.3% and 8.8%; Formula I I handles back spike number, Dan Suichong, sugar-acid ratio, soluble solid content and pericarp anthocyanin content and compares respectively according to having increased by 14.9%, 19.6%, 16.6%, 13.5% and 17.6%.
Can know on having, with formula I I handle to grape biology amount and quality to improve effect best.
Embodiment 7: based on the plant resource biocide preparation of thymol at preventive and therapeutic effect to bitter rot or anthracnose of grape.
Formula I: 50% carbendazol wettable powder.
Formula I I:75% tpn wetting powder.
The plant resource biocide preparation of the thymol that formula I II: embodiment 1 makes.
Choose four processing of morbidity field piece design: handle 1: contrast (contrast CK is a clear water); Handle 2: formula I; Handle 3: formula I I; Handle 4: formula I II.Each handles repetition 3 times, and treatment fluid is every to spray once at a distance from 5d, carries out statistical analysis after spraying 3 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. the Henan agricultural science, and 2010,4:77-79.)
Can know that by table 2 on grape, formula I is 37.00% to the preventive effect of bitter rot or anthracnose of grape; Formula I I is 47.80% to the preventive effect of bitter rot or anthracnose of grape; The preventive effect of formula I II bitter rot or anthracnose of grape is 78.68%.
By on can know, handle best to the control efficiency of bitter rot or anthracnose of grape with formula I I.
Table 2 different disposal is to the bitter rot or anthracnose of grape control efficiency
Claims (5)
1. a plant resource biocide preparation is characterized in that, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%.
2. plant resource biocide preparation according to claim 1; It is characterized in that described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
3. the preparation method of the described plant resource biocide preparation of claim 1; It is characterized in that; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Thymol added mix the formation solution A in the part ethanol, again emulsifier and azone are formed solution B with the residue dissolve with ethanol, then solution A, solution B and water are mixed.
4. the application of the described plant resource biocide preparation of claim 1 in promoting grape growth.
5. the application of the described plant resource biocide preparation of claim 1 in preventing and treating bitter rot or anthracnose of grape.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104002040A CN102365939B (en) | 2011-12-06 | 2011-12-06 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104002040A CN102365939B (en) | 2011-12-06 | 2011-12-06 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
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| Publication Number | Publication Date |
|---|---|
| CN102365939A true CN102365939A (en) | 2012-03-07 |
| CN102365939B CN102365939B (en) | 2013-04-17 |
Family
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|---|---|---|---|
| CN2011104002040A Expired - Fee Related CN102365939B (en) | 2011-12-06 | 2011-12-06 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
| CN109258637A (en) * | 2018-10-17 | 2019-01-25 | 四川省兰月科技有限公司 | Sterilize the composition pesticide of control shoot |
| CN109526530A (en) * | 2018-11-21 | 2019-03-29 | 邓思浩 | The implantation methods of pollution-free grape |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001310214A (en) * | 2000-04-28 | 2001-11-06 | Aubex Corp | Lengthy material working device and lengthy material working method |
| CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
| CN102365940A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling pepper anthracnose |
| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
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| CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001310214A (en) * | 2000-04-28 | 2001-11-06 | Aubex Corp | Lengthy material working device and lengthy material working method |
| CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
| CN102365940A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling pepper anthracnose |
| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
Non-Patent Citations (1)
| Title |
|---|
| 张静等: "《百里香酚抑菌活性初探》", 《中国农学通报》, vol. 25, no. 21, 31 December 2009 (2009-12-31), pages 277 - 280 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
| CN109258637A (en) * | 2018-10-17 | 2019-01-25 | 四川省兰月科技有限公司 | Sterilize the composition pesticide of control shoot |
| CN109526530A (en) * | 2018-11-21 | 2019-03-29 | 邓思浩 | The implantation methods of pollution-free grape |
| CN109526530B (en) * | 2018-11-21 | 2021-06-08 | 靳职雄 | Planting method of pollution-free grapes |
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| CN102365939B (en) | 2013-04-17 |
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