CN102388923B - Botanical bactericidal preparation and application of preparation in blight control - Google Patents
Botanical bactericidal preparation and application of preparation in blight control Download PDFInfo
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- CN102388923B CN102388923B CN2011104002089A CN201110400208A CN102388923B CN 102388923 B CN102388923 B CN 102388923B CN 2011104002089 A CN2011104002089 A CN 2011104002089A CN 201110400208 A CN201110400208 A CN 201110400208A CN 102388923 B CN102388923 B CN 102388923B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 8
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims abstract description 52
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000005844 Thymol Substances 0.000 claims abstract description 26
- 229960000790 thymol Drugs 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 24
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims abstract description 17
- 241000223259 Trichoderma Species 0.000 claims abstract description 13
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 35
- 230000001954 sterilising effect Effects 0.000 claims description 35
- 238000004659 sterilization and disinfection Methods 0.000 claims description 35
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 19
- -1 polyoxyethylene Polymers 0.000 claims description 17
- 240000008067 Cucumis sativus Species 0.000 claims description 10
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 8
- 241000223218 Fusarium Species 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 7
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 claims description 6
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 6
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 5
- 240000003768 Solanum lycopersicum Species 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- 235000002566 Capsicum Nutrition 0.000 claims description 4
- 240000008574 Capsicum frutescens Species 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 244000061458 Solanum melongena Species 0.000 claims description 4
- 235000002597 Solanum melongena Nutrition 0.000 claims description 4
- 244000046738 asparagus lettuce Species 0.000 claims description 4
- 235000006705 asparagus lettuce Nutrition 0.000 claims description 4
- 150000008107 benzenesulfonic acids Chemical class 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- 239000001390 capsicum minimum Substances 0.000 claims description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 3
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 3
- 239000004359 castor oil Substances 0.000 claims description 3
- 235000019438 castor oil Nutrition 0.000 claims description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229920001568 phenolic resin Polymers 0.000 claims description 3
- 230000008635 plant growth Effects 0.000 claims description 3
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims description 3
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 244000241235 Citrullus lanatus Species 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000012010 growth Effects 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 241000607479 Yersinia pestis Species 0.000 abstract description 2
- 239000000284 extract Substances 0.000 abstract description 2
- 230000000749 insecticidal effect Effects 0.000 abstract description 2
- 241000219109 Citrullus Species 0.000 description 33
- 238000000034 method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 10
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 230000003449 preventive effect Effects 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 229930002875 chlorophyll Natural products 0.000 description 6
- 235000019804 chlorophyll Nutrition 0.000 description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 5
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000508192 Fusarium oxysporum f. sp. niveum Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 239000005842 Thiophanate-methyl Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000000853 biopesticidal effect Effects 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000005839 Tebuconazole Substances 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a botanical bactericidal preparation. The botanical bactericidal preparation comprises the following components, by weight, 0.1-50% of thymol, 5-30% of an emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water, and 1ml of the botanical bactericidal preparation comprises Trichoderma spp with the number of 1*10<7-9>. The invention also discloses a preparation method of the botanical bactericidal preparation and an application of the botanical bactericidal preparation in the blight control. The thymol-based botanical bactericidal preparation of the invention, which has effective components from plants with the insecticidal function or extracts from the plants, allows the blight of crops to be effectively controlled, and has the multiple efficacies of crop growth promotion, yield and income increase, quality improvement, nutrient utilization rate improvement, pest control, no pollution, and the like, is suitable for the production of nuisance-free, green and organic agricultural products.
Description
Technical field
The invention belongs in agricultural production the fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of botanical sterilization preparation and the application in preventing and treating fusarium wilt thereof.
Background technology
Watermelon blight claims again the watermelon dead arm, withers and transfer sick, wilt disease, be a kind of be the worldwide soil-borne disease that is caused by Fusarium oxysporum f. sp. niveum (Fusarium oxysporum f.sp.niveum), be one of Major Diseases of watermelon production, have the title of " watermelon cancer ".Particularly in recent years, in each watermelon of China producing region, generation is arranged all.Along with the growth of watermelon enlarged areas, cropping pattern is single in addition, unreasonable crop rotation, continuous cropping obstacle increases the weight of, cause causing harm of watermelon blight to be and increase the weight of year by year trend, the general time incidence of disease is 20%～40%, and the serious time reaches even total crop failure more than 80%, and watermelon blight has become the Major Diseases in watermelon production.Watermelon blight is the soil-borne disease that is caused by Fusarium oxysporum f. sp. niveum, all can occur from going out seedling to watermelon maturation, and is climing the heaviest to the morbidity of knot melon phase to bloom, to take out.Also normal some branches and tendrils wilting in one strain, another part branches and tendrils are normal growth still, spreads to complete stool gradually later on.The master who also has is climing withered, and grows adventive root at basal part of stem, grows overgrowing branch; The half of morbidity of same branches and tendrils that also has, half of sound.But sharply the time, the climing 3-4d of stem is namely withered when patient's condition.Chlamydospore more than Survival for 10 Years, leaves the host and also can survive 5～6 years in soil, in the situation that long-term planting, pathogen accumulates in a large number, causes the control difficulty.Therefore, taking the effectively preventing method, alleviate its harm, is increasing yield of water melons, the key that increases income.
At present on the means of control watermelon blight, main or chemical control, this is because chemical control has instant effect, effective characteristics.Domestic 75% thiophanate methyl that screens, 50% carbendazim, 75% tpn, 50% " shuang xiao ling ", 50%DT bactericide, 68.4% TOEFL are beautiful, and can kill, the medicament such as anti-wither chemical has certain control efficiency to watermelon blight.But the most medicament frequency of utilization is higher, and height is malicious, use persistent pesticide is still of common occurrence, and environment and people's health in serious harm; Long-time unreasonable use chemical pesticide control watermelon blight causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase the fruit Pesticide Residues, and contaminated environment increases production cost.
The biopesticide control is because its selectivity is strong, little to eco-environmental impact, and therefore developing efficient, environmentally friendly botanical biological pesticide control watermelon blight is the focus of present research.By the biopesticide of making after the improvement of vegetable source natural compound, to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, have efficient noresidue on the one hand, without poisoning, the advantages such as the non-resistant property of medicine; Can effectively improve quality of agricultural product on the other hand when controlling disease.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of botanical sterilization preparation of thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve is to provide the preparation method of above-mentioned botanical sterilization preparation based on thymol.
The technical problem that the present invention will solve at last is to provide the application of above-mentioned botanical sterilization preparation in preventing and treating fusarium wilt.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%, and described botanical sterilization preparation contains Trichoderma 1 * 10
7-9Individual/ml.
Above-mentioned botanical sterilization preparation preferably comprises the component of following percetage by weight: thymol 0.5～5%, and emulsifier 10～20%, azone 2～3%, ethanol 20～40%, water 32～67.5%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole, tpn.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol is added mix the formation solution A in part ethanol, again emulsifier and azone are formed solution B with the dissolving of residue ethanol, then solution A, solution B and water are mixed, add Trichoderma to bacterium amount in the solution and reach 1 * 10
7-9Individual/ml.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and the consumption sum of twice is the ethanol raw material total amount of formula ratio.
The application of above-mentioned botanical sterilization preparation in promoting plant growth, wherein, described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant or tomato.
The application of above-mentioned botanical sterilization preparation in control crop fusarium wilt, wherein, described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant, tomato or paddy rice.
Beneficial effect: its effective component of botanical sterilization preparation based on thymol of the present invention derives from plant or its extract with insecticidal function, not only can effectively prevent and treat the fusarium wilt of crop, have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, the multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect is significantly, raw material are easy to get, production technology is simple, market potential is large, economic benefit and social benefit obvious, have very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, embodiment is described only is used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is phenethyl phenol polyoxyethylene poly-oxygen propylene aether) 20%, azone 3%, ethanol 40%, water 35%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol is added in 1/4 ethanol of measuring mix the formation solution A, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 3/4 amount, then solution A, solution B and water are mixed, add Trichoderma to bacterium amount in the solution and reach 1 * 10
8Individual/ml.
Embodiment 2:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 1%, emulsifier (emulsifier is dialkyl benzene sulfonic acids calcium) 10%, azone 2%, ethanol 30%, water 57%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol is added in 1/2 ethanol of measuring mix the formation solution A, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 1/2 amount, then solution A, solution B and water are mixed, add Trichoderma to bacterium amount in the solution and reach 1 * 10
8Individual/ml.
Embodiment 3:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is phenethyl phenol polyethenoxy ether and alkylphenol polyoxyethylene phosphate by weight the mixture of 1: 2) 10%, azone 2%, ethanol 20%, water 67.5%, and described botanical sterilization preparation contains Trichoderma 1 * 10
9Individual/ml.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct and aralkyl phenol polyethenoxy ether by weight the mixture of 1: 1: 1) 30%, azone 20%, ethanol 10%, water 39.9%, and described botanical sterilization preparation contains Trichoderma 1 * 10
7Individual/ml.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium by weight the mixture of 1: 1: 1) 5%, azone 0.1%, ethanol 20%, water 24.9%, and described botanical sterilization preparation contains Trichoderma 1 * 10
7Individual/ml.
The preparation method is with embodiment 2.
Embodiment 6: based on the botanical sterilization preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The botanical sterilization preparation of the thymol that formula I I: embodiment 1 prepares.
Seed is with the H of 0.3% (w/w)
2O
2After sterilization, clean with distilled water, then select the culture dish (diameter 15cm) that homogeneous, the normal seed of form are placed in paving 3 metafiltration paper, train seedling.Wait the rear 5d of emerging, choose the consistent material of growing way and transfer them in the plastic basin alms bowl, after seedling age 20d, spray with containing different disposal liquid respectively, each is processed and repeats 3 times.Treatment fluid sprays once every 5d, samples after 60d, measures and respectively processes green vegetables relevant physiological biochemical indicator.(annotate: contrast CK is clear water)
Assay method:
Fresh weight is measured: after off-test, then 3 more consistent seedling of upgrowth situation of the random picking of every basin are cleaned with deionized water and blot its fresh weight of rear measurement.
Chlorophyll content: get 0.2g left and right blade, with WFZ UV-4802H ultraviolet-uisible spectrophotometer [Long Nike (Shanghai) Instr Ltd.], measure with reference to the Wellburn method.(Wellburn?A?R.The?spectral?determinationof?chlorophylls?a?and?b，as?well?as?total?carotenoids?using?various?solvents?withspectrophotometers?of?different?resolution.J.Plant?Physiol.，1994，144：307-313.)
The impact of table 1 different disposal on watermelon and cucumber biomass
As shown in Table 1, watermelon fresh weight, dry weight, plant height, water content and chlorophyll content after formula I is processed increased by 17.4%, 3.1%, 12.0%, 5.3% and 7.7% than contrast respectively; After formula I I processes, fresh weight, dry weight, plant height, water content and chlorophyll content have increased by 56.1%, 18.1%, 38.6%, 10.6% and 14.3% than contrast respectively.Cucumber fresh weight, dry weight, plant height, water content and chlorophyll content after formula I is processed have increased by 20.2%, 5.7%, 13.3%, 5.7% and 6.5% than contrast respectively; After formula I I processes, fresh weight, dry weight, plant height, water content and chlorophyll content have increased by 33.0%, 33.1%, 42.9%, 11.4% and 12.1% than contrast respectively.
As can be known, process best to watermelon and cucumber biomass effect with formula I I on having.
Embodiment 7: based on the botanical sterilization preparation of thymol at the preventive and therapeutic effect to watermelon blight.
Formula I: potassium permanganate.
Formula I I:50% carbendazol wettable powder.
Formula I II:70% thiophanate-methyl wetting powder.
The botanical sterilization preparation formula of the thymol of formula I V: embodiment 1.
Choose four processing of morbidity dimension design of farmland: process 1: contrast (contrast CK is clear water); Process 2: formula I; Process 3: formula I I; Process 4: formula I II; Process 5: formula I V.Each process to repeat 3 times, and treatment fluid is filled with root once every 10d, carries out statistical analysis after filling with root 2 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(control efficiency of Dou Rui wood .3 plants endogenetic bacterium to graw mold of tomato. Henan Agricultural Sciences, 2010,4:77-79.)
As shown in Table 2, on watermelon, formula I is 51.1% to the preventive effect of watermelon blight; Formula I I is 52.2% to the preventive effect of watermelon blight; Formula I II is 55.1% to the preventive effect of watermelon blight; Formula I V is 77.6% to the preventive effect of watermelon blight.On cucumber, formula I is 54.51% to the preventive effect of watermelon blight; Formula I I is 55.71% to the preventive effect of watermelon blight; Formula I II is 58.77% to the preventive effect of watermelon blight; Formula I V is 82.47% to the preventive effect of watermelon blight.
As from the foregoing, process best to the control efficiency of watermelon and cucumber fusarium axysporum with formula I V.
Table 2 different disposal is to watermelon and cucumber fusarium axysporum control efficiency
Claims (6)
1. a botanical sterilization preparation, is characterized in that, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%, and described botanical sterilization preparation contains Trichoderma 1 * 10
7-9Individual/ml;
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium.
2. the preparation method of botanical sterilization preparation claimed in claim 1, it is characterized in that, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol is added mix the formation solution A in part ethanol, again emulsifier and azone are formed solution B with the dissolving of residue ethanol, then solution A, solution B and water are mixed, add Trichoderma to bacterium amount in the solution and reach 1 * 10
7-9Individual/ml.
3. the application of botanical sterilization preparation claimed in claim 1 in promoting plant growth.
4. application according to claim 3 is characterized in that described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant or tomato.
5. the application of botanical sterilization preparation claimed in claim 1 in control crop fusarium wilt.
6. application according to claim 5 is characterized in that described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant, tomato or paddy rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104002089A CN102388923B (en) | 2011-12-06 | 2011-12-06 | Botanical bactericidal preparation and application of preparation in blight control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104002089A CN102388923B (en) | 2011-12-06 | 2011-12-06 | Botanical bactericidal preparation and application of preparation in blight control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102388923A CN102388923A (en) | 2012-03-28 |
| CN102388923B true CN102388923B (en) | 2013-06-12 |
Family
ID=45856418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104002089A Expired - Fee Related CN102388923B (en) | 2011-12-06 | 2011-12-06 | Botanical bactericidal preparation and application of preparation in blight control |
Country Status (1)
| Country | Link |
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| CN (1) | CN102388923B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688934B (en) * | 2013-12-18 | 2016-05-04 | 重庆市优胜科技发展有限公司 | Promote crop yield dry to improve antiviral multifunctional preparation of local flavor and preparation method thereof |
| EP3258782A4 (en) * | 2015-02-16 | 2018-07-25 | Laboratoire M2 | Aqueous compositions for controlling pests or regulating plant growth, said compositions comprising thymol or carvacrol, a surfactant, and a solvent |
| CN107926526A (en) * | 2017-11-20 | 2018-04-20 | 焦杰彪 | The method of one prevention watermelon blight |
| CN108244157B (en) * | 2018-01-24 | 2020-03-31 | 黑龙江省动物疫病预防与控制中心 | Biological disinfectant for animals |
| CN114903059A (en) * | 2022-06-21 | 2022-08-16 | 广东省农业科学院植物保护研究所 | Thymus vulgaris oil and application of preparation thereof in preventing and treating crop soft rot |
-
2011
- 2011-12-06 CN CN2011104002089A patent/CN102388923B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN102388923A (en) | 2012-03-28 |
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