CN102388923A - Botanical bactericidal preparation and application of preparation in blight control - Google Patents
Botanical bactericidal preparation and application of preparation in blight control Download PDFInfo
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- CN102388923A CN102388923A CN2011104002089A CN201110400208A CN102388923A CN 102388923 A CN102388923 A CN 102388923A CN 2011104002089 A CN2011104002089 A CN 2011104002089A CN 201110400208 A CN201110400208 A CN 201110400208A CN 102388923 A CN102388923 A CN 102388923A
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- plant resource
- thymol
- emulsifier
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses a botanical bactericidal preparation. The botanical bactericidal preparation comprises the following components, by weight, 0.1-50% of thymol, 5-30% of an emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water, and 1ml of the botanical bactericidal preparation comprises Trichoderma spp with the number of 1*10<7-9>. The invention also discloses a preparation method of the botanical bactericidal preparation and an application of the botanical bactericidal preparation in the blight control. The thymol-based botanical bactericidal preparation of the invention, which has effective components from plants with the insecticidal function or extracts from the plants, allows the blight of crops to be effectively controlled, and has the multiple efficacies of crop growth promotion, yield and income increase, quality improvement, nutrient utilization rate improvement, pest control, no pollution, and the like, is suitable for the production of nuisance-free, green and organic agricultural products.
Description
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of plant resource biocide preparation and the application in preventing and treating fusarium wilt thereof.
Background technology
Watermelon blight is claimed the watermelon dead arm again, is withered and transfer sick, wilt disease; Be a kind of be the worldwide soil-borne disease that causes by Fusarium oxysporum watermelon specialized form (Fusarium oxysporum f.sp.niveum); Be one of main disease of watermelon production, have the title of " watermelon cancer ".Particularly in recent years, in each watermelon producing region of China generation is arranged all.Along with the growth of watermelon enlarged areas; Cropping pattern is single in addition, unreasonable crop rotation, and continuous cropping obstacle increases the weight of; Cause causing harm of watermelon blight to be and increase the weight of trend year by year; The general time incidence of disease is 20%～40%, and the serious time reaches more than 80% even total crop failure, and watermelon blight has become the main disease in the watermelon production.Watermelon blight is the soil-borne disease that is caused by Fusarium oxysporum watermelon specialized form, all can take place from going out seedling to watermelon maturation, and is climing the heaviest to the morbidity of knot melon phase to bloom, to take out.Also normal some branches and tendrils are wilted in one strain, and another part branches and tendrils are normal growth still, spreads to complete stool gradually later on.The master who also has is climing withered, and grows adventive root at basal part of stem, grows overgrowing branch; The half of morbidity of same branches and tendrils that also has, half of sound.But when patient's condition was rapid, the climing 3-4d of stem was promptly withered.Chlamydospore survives more than 10 years in soil, leaves the host and also can survive 5～6 years, and under the situation of long-term planting, pathogen accumulates in a large number, causes the control difficulty.Therefore, taking the effectively preventing method, alleviate its harm, is increasing yield of water melons, the key that increases income.
Present on the means of control watermelon blight, mainly still chemical control, this is because chemical control has instant effect, good effectiveness.Domestic 75% thiophanate methyl that screens, 50% carbendazim, 75% tpn, 50% " shuang xiao ling ", 50%DT bactericide, 68.4% TOEFL are beautiful, and can kill, medicament such as anti-wither chemical all has certain control efficiency to watermelon blight.But the most medicament frequency of utilization is higher, and high use malicious, persistent pesticide is still of common occurrence, and environment and people's health in serious harm; Long-time unreasonable use chemical pesticide control watermelon blight causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase residue of pesticide in the fruit, and contaminated environment increases production cost.
The biopesticide control is owing to its selectivity is strong, little to eco-environmental impact, and therefore developing out efficient, environmentally friendly botanical biological pesticide control watermelon blight is the focus of studying at present.Through the biopesticide of processing after the improvement of vegetable source natural compound, to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, no poisoning, advantages such as the non-resistant property of medicine on the one hand; In the control disease, can effectively improve quality of agricultural product on the other hand.
Summary of the invention
Technical problem to be solved by this invention provides a kind of plant resource biocide preparation of thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned plant resource biocide preparation based on thymol.
The technical problem that the present invention will solve at last provides the application of above-mentioned plant resource biocide preparation in preventing and treating fusarium wilt.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%; Azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%; And described plant resource biocide preparation contains Trichoderma 1 * 10
7-9Individual/ml.
Above-mentioned plant resource biocide preparation preferably comprises the component of following percetage by weight: thymol 0.5～5%, and emulsifier 10～20%, azone 2～3%, ethanol 20～40%, water 32～67.5%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole, tpn.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Thymol added mix the formation solution A in the part ethanol; Again emulsifier and azone are formed solution B with the residue dissolve with ethanol, then solution A, solution B and water are mixed, in solution, add Trichoderma to bacterium amount and reach 1 * 10
7-9Individual/ml.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned plant resource biocide preparation in promoting plant growth, wherein, described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant or tomato.
The application of above-mentioned plant resource biocide preparation in control crop fusarium wilt, wherein, described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant, tomato or paddy rice.
Beneficial effect: its effective component of plant resource biocide preparation based on thymol of the present invention derives from plant or its extract with insecticidal function; Not only can effectively prevent and treat the fusarium wilt of crop; Have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is big, economic benefit and social benefit are obvious, has the wide application prospect of ten minutes.
Embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that embodiment is described only to be used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is a phenethyl phenol polyoxyethylene poly-oxygen propylene aether) 20%; Azone 3%, ethanol 40%, water 35%; And described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/4 amount; Again emulsifier and azone are formed solution B with the dissolve with ethanol that remains 3/4 amount, then solution A, solution B and water are mixed, in solution, add Trichoderma to bacterium amount and reach 1 * 10
8Individual/ml.
Embodiment 2:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 1%, emulsifier (emulsifier is a dialkyl benzene sulfonic acids calcium) 10%, azone 2%, ethanol 30%, water 57%, and described plant resource biocide preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/2 amount; Again emulsifier and azone are formed solution B with the dissolve with ethanol that remains 1/2 amount, then solution A, solution B and water are mixed, in solution, add Trichoderma to bacterium amount and reach 1 * 10
8Individual/ml.
Embodiment 3:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is phenethyl phenol polyethenoxy ether and APES phosphate by weight 1: 2 mixture) 10%, azone 2%; Ethanol 20%; Water 67.5%, and described plant resource biocide preparation contains Trichoderma 1 * 10
9Individual/ml.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct and aralkyl phenol polyethenoxy ether by weight 1: 1: 1 mixture) 30%, azone 20%; Ethanol 10%; Water 39.9%, and described plant resource biocide preparation contains Trichoderma 1 * 10
7Individual/ml.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium by weight 1: 1: 1 mixture) 5%, azone 0.1%; Ethanol 20%; Water 24.9%, and described plant resource biocide preparation contains Trichoderma 1 * 10
7Individual/ml.
The preparation method is with embodiment 2.
Embodiment 6: based on the plant resource biocide preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The plant resource biocide preparation of the thymol that formula I I: embodiment 1 prepares.
Seed is with the H of 0.3% (w/w)
2O
2After the sterilization, clean, select the culture dish (diameter 15cm) that homogeneous, the normal seed of form place shop 3 metafiltration paper then, train seedling with distilled water.5d after waiting to emerge chooses the consistent material of growing way it is transferred in the plastic basin alms bowl, behind the seedling age 20d, sprays with containing different disposal liquid respectively, and each handles repetition 3 times.Treatment fluid is every to spray once at a distance from 5d, samples behind the 60d, measures and respectively handles green vegetables relevant physiological biochemical indicator.(annotate: contrast CK is a clear water)
Assay method:
Fresh weight is measured: after the off-test, every basin is the consistent seedling of 3 upgrowth situations of picking at random, measures its fresh weight with the clean back of blotting of deionized water then.
Chlorophyll content: get 0.2g left and right sides blade,, measure with reference to the Wellburn method with WFZ UV-4802H ultraviolet-uisible spectrophotometer [Long Nike (Shanghai) Instr Ltd.].(Wellburn?A?R.The?spectral?determinationof?chlorophylls?a?and?b，as?well?as?total?carotenoids?using?various?solvents?withspectrophotometers?of?different?resolution.J.Plant?Physiol.，1994，144：307-313.)
Table 1 different disposal is to the influence of watermelon and cucumber biomass
Can know that by table 1 watermelon is handled back fresh weight, dry weight, plant height, water content and chlorophyll content in formula I and compared respectively according to having increased by 17.4%, 3.1%, 12.0%, 5.3% and 7.7%; Formula I I handles back fresh weight, dry weight, plant height, water content and chlorophyll content and compares respectively according to having increased by 56.1%, 18.1%, 38.6%, 10.6% and 14.3%.Cucumber is handled back fresh weight, dry weight, plant height, water content and chlorophyll content in formula I and compares respectively according to having increased by 20.2%, 5.7%, 13.3%, 5.7% and 6.5%; Formula I I handles back fresh weight, dry weight, plant height, water content and chlorophyll content and compares respectively according to having increased by 33.0%, 33.1%, 42.9%, 11.4% and 12.1%.
Can know on having, handle best watermelon and cucumber biomass effect with formula I I.
Embodiment 7: based on the plant resource biocide preparation of thymol at preventive and therapeutic effect to watermelon blight.
Formula I: potassium permanganate.
Formula I I:50% carbendazol wettable powder.
Formula I II:70% thiophanate-methyl wetting powder.
The plant resource biocide preparation prescription of the thymol of formula I V: embodiment 1.
Choose four processing of morbidity field piece design: handle 1: contrast (contrast CK is a clear water); Handle 2: formula I; Handle 3: formula I I; Handle 4: formula I II; Handle 5: formula I V.Each handles repetition 3 times, and treatment fluid is every irritates root once at a distance from 10d, carries out statistical analysis after irritating root 2 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. the Henan agricultural science, and 2010,4:77-79.)
Can know that by table 2 on watermelon, formula I is 51.1% to the preventive effect of watermelon blight; Formula I I is 52.2% to the preventive effect of watermelon blight; Formula I II is 55.1% to the preventive effect of watermelon blight; Formula I V is 77.6% to the preventive effect of watermelon blight.On cucumber, formula I is 54.51% to the preventive effect of watermelon blight; Formula I I is 55.71% to the preventive effect of watermelon blight; Formula I II is 58.77% to the preventive effect of watermelon blight; Formula I V is 82.47% to the preventive effect of watermelon blight.
By on can know, handle best to the control efficiency of watermelon and cucumber fusarium axysporum with formula I V.
Table 2 different disposal is to watermelon and cucumber fusarium axysporum control efficiency
Claims (7)
1. a plant resource biocide preparation is characterized in that, it comprises the component of following percetage by weight: thymol 0.1～50%; Emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%; Water 0.1～84.8%, and described plant resource biocide preparation contains Trichoderma 1 * 10
7-9Individual/ml.
2. plant resource biocide preparation according to claim 1; It is characterized in that described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
3. the preparation method of the described plant resource biocide preparation of claim 1; It is characterized in that; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol is added mix the formation solution A in the part ethanol, again with emulsifier and azone with residue dissolve with ethanol formation solution B; Then solution A, solution B and water are mixed, in solution, add Trichoderma to bacterium amount and reach 1 * 10
7-9Individual/ml.
4. the application of the described plant resource biocide preparation of claim 1 in promoting plant growth.
5. application according to claim 4 is characterized in that described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant or tomato.
6. the application of the described plant resource biocide preparation of claim 1 in control crop fusarium wilt.
7. application according to claim 6 is characterized in that described crop is watermelon, Kidney bean, asparagus lettuce, capsicum, cucumber, eggplant, tomato or paddy rice.
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| CN2011104002089A CN102388923B (en) | 2011-12-06 | 2011-12-06 | Botanical bactericidal preparation and application of preparation in blight control |
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Cited By (5)
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| CN103688934A (en) * | 2013-12-18 | 2014-04-02 | 重庆市优胜科技发展有限公司 | Antiviral multifunctional preparation for increasing dry matter of plants and improving flavor as well as preparation method thereof |
| US20180014534A1 (en) * | 2015-02-16 | 2018-01-18 | Laboratoire M2 | Composition for use as pesticide, fertilizer, or both, and uses thereof |
| CN107926526A (en) * | 2017-11-20 | 2018-04-20 | 焦杰彪 | The method of one prevention watermelon blight |
| CN108244157A (en) * | 2018-01-24 | 2018-07-06 | 黑龙江省动物疫病预防与控制中心 | Animal biological antiseptic medicament |
| CN114903059A (en) * | 2022-06-21 | 2022-08-16 | 广东省农业科学院植物保护研究所 | Thymus vulgaris oil and application of preparation thereof in preventing and treating crop soft rot |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688934A (en) * | 2013-12-18 | 2014-04-02 | 重庆市优胜科技发展有限公司 | Antiviral multifunctional preparation for increasing dry matter of plants and improving flavor as well as preparation method thereof |
| CN103688934B (en) * | 2013-12-18 | 2016-05-04 | 重庆市优胜科技发展有限公司 | Promote crop yield dry to improve antiviral multifunctional preparation of local flavor and preparation method thereof |
| US20180014534A1 (en) * | 2015-02-16 | 2018-01-18 | Laboratoire M2 | Composition for use as pesticide, fertilizer, or both, and uses thereof |
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| US10912294B2 (en) * | 2015-02-16 | 2021-02-09 | Laboratoire M2 | Composition for use as pesticide, fertilizer, or both, and uses thereof |
| CN107926526A (en) * | 2017-11-20 | 2018-04-20 | 焦杰彪 | The method of one prevention watermelon blight |
| CN108244157A (en) * | 2018-01-24 | 2018-07-06 | 黑龙江省动物疫病预防与控制中心 | Animal biological antiseptic medicament |
| CN108244157B (en) * | 2018-01-24 | 2020-03-31 | 黑龙江省动物疫病预防与控制中心 | Biological disinfectant for animals |
| CN114903059A (en) * | 2022-06-21 | 2022-08-16 | 广东省农业科学院植物保护研究所 | Thymus vulgaris oil and application of preparation thereof in preventing and treating crop soft rot |
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