CN102365940B - Botanical sterilization preparation and application thereof for controlling pepper anthracnose - Google Patents

Botanical sterilization preparation and application thereof for controlling pepper anthracnose Download PDF

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CN102365940B
CN102365940B CN 201110400391 CN201110400391A CN102365940B CN 102365940 B CN102365940 B CN 102365940B CN 201110400391 CN201110400391 CN 201110400391 CN 201110400391 A CN201110400391 A CN 201110400391A CN 102365940 B CN102365940 B CN 102365940B
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sterilization preparation
botanical sterilization
preparation
emulsifier
thymol
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CN102365940A (en
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薛延丰
石志琦
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Abstract

The invention discloses a botanical sterilization preparation, which contains the following components in percentage by weight: 0.1-50% of thymol, 5-30% of emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water. The invention further discloses a preparation method and application of the botanical sterilization preparation to control of pepper anthracnose. Pharmaceutical components of the botanical sterilization preparation based on thymol come from plants or extractives thereof with an insecticidal function; the botanical sterilization preparation not only can be used for effectively controlling the pepper anthracnose but also can be used for promoting growth and development of crops, increasing production, increasing income, improving quality, increasing nutrient utilization rate, controlling injurious insects, avoiding pollution and the like; the botanical sterilization preparation is applied to the production of nuisanceless, green and organic agricultural products, is obvious in application effect, simple in production process, high in market potential, and obvious in economic benefit and social benefit, and has very wide application prospect; and raw materials are easy to obtain.

Description

A kind of botanical sterilization preparation and the application in the control pepper anthracnose thereof
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of botanical sterilization preparation and the application in the control pepper anthracnose thereof.
Background technology
Pepper anthracnose (Colletotrichum capsici) is that the speciogenesis on capsicum, the pimento is general and endanger serious worldwide disease.In recent years, along with the continuous expansion of pepper planting area, the disease of capsicum trend multiple and that increase the weight of is day by day obvious, and therefore the yield and quality of capsicum is greatly affected.Pepper anthracnose is the regular incidence evil on capsicum produces, distribute general, especially in the rainy time, main harm fruit and blade are the main causes that causes capsicum " three fall ", usually diseased fruit rate is about 10%, diseased fruit rate reaches 30%~40% when serious, but the underproduction can cause that pepper seedling is dead more than 50%, leaf really rots, and is all influential to Quality of Capsicum, output.Pepper anthracnose is several kinds of caused fungal diseases in the Deuteromycotina colletotrichum (Colletotrichum Cord).Pepper anthracnose can cause harm Pepper Leaves and fruit show the circular spot of water soaking mode brown, verticillate pore on the scab at sick leaf; Sick fruit is upper to be the circular spot of water soaking mode brown, and scab is projection gradually, forms taupe, black or orange red granule point, is arranged in the concentric wheel stripe spot, and pale red or orange red mucilage are often overflowed in disease section during high humidity, i.e. conidium.The drying shrinkage attenuation of disease section is easily broken when dry.Pepper anthracnose can be divided into red anthrax bacteria, black anthracnose bacterium and stain anthrax bacteria according to the disease symptom.
Present on the means of control pepper anthracnose, main or chemical control, chemical control is to prevent and treat pepper anthracnose with bactericide.Efficient, quick-acting, easy to use, economic benefit advantages of higher that bactericide has, but only have protective effect, nothing is treated and is rooted out effect, and the lasting period is short, and application times is many, and germ has produced pesticide resistance to it; Long-time unreasonable use chemical pesticide control pepper anthracnose causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase the fruit Pesticide Residues, and contaminated environment increases production cost.Chemical pesticide still is difficult to effectively solve to people and animals' toxic and side effect and residue problem so far.
Along with improve constantly with various countries the pay attention to day by day to protect mankind living environment of the world today to crops quality and quantity demand; and the deepening continuously of sustainable agriculture and lasting plant protection idea; the production of people's growing interest environmental problem and pollution-free food; developing efficient, environmentally friendly botanical biological pesticide control pepper anthracnose is the focus of studying at present, also is the trend of future development.By the biopesticide of making after the improvement of vegetable source natural compound, not only control efficiency is good, also to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, without poisoning, and the advantages such as the non-resistant property of medicine; Simultaneously can the Effective Raise quality of agricultural product in the control disease.
Summary of the invention
Technical problem to be solved by this invention provides a kind of botanical sterilization preparation based on thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned botanical sterilization preparation.
The technical problem that the present invention will solve at last provides the application of above-mentioned botanical sterilization preparation in the control pepper anthracnose.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1~50%, emulsifier 5~30%, azone 0.1~20%, ethanol 10~60%, water 0.1~84.8%.
Above-mentioned botanical sterilization preparation preferably comprises the component of following percetage by weight: thymol 0.5~5%, emulsifier 10~20%, azone 1~2%, ethanol 20~40%, water 33~68%.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol added mix the formation solution A in the part ethanol, again emulsifier and azone are formed solution B with the dissolving of residue ethanol, then solution A, solution B and water are mixed and get final product.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned botanical sterilization preparation in promoting chili growth.
The application of above-mentioned botanical sterilization preparation in the control pepper anthracnose.
Beneficial effect: its effective component of botanical sterilization preparation based on thymol of the present invention derives from plant or its extract with insecticidal function, can not only effectively prevent and treat pepper anthracnose, have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, the multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is large, economic benefit and social benefit are obvious, has very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, embodiment is described only to be used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is the phenethyl phenol polyethenoxy ether) 20%, azone 2%, ethanol 30%, water 46%.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/4 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 3/4 amount, then solution A, solution B and water are mixed and get final product.
Embodiment 2:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 5%, emulsifier (emulsifier is that emulsifier is aralkyl phenol polyethenoxy ether phosphate and alkylphenol polyoxyethylene phosphate by weight 1: 1 mixture) 20%, azone 4%, ethanol 40%, water 31%.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/2 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 1/2 amount, then solution A, solution B and water are mixed and get final product.
Embodiment 3:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is the alkylphenol polyoxyethylene phosphate) 10%, azone 2%, ethanol 20%, water 67.5%.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium and castor oil ethylene oxide adduct by weight 1: 2 mixture) 30%, azone 0.1%, ethanol 60%, water 9.8%.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether and alkyl phenol formaldehyde resin polyoxyethylene ether by weight 1: 2: 1 mixture) 5%, azone 20%, ethanol 10%, water 15%.
The preparation method is with embodiment 2.
Embodiment 6: based on the botanical sterilization preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The botanical sterilization preparation of the thymol that formula I I: embodiment 1 makes.
Seed is with the H of 0.3% (w/w) 2O 2After the sterilization, clean with distilled water, then select homogeneous, the normal seed of form places the culture dish (diameter 15em) that spreads 3 metafiltration paper, trains seedling.Wait the rear 5d of emerging, choose the consistent material of growing way and transfer them in the plastic basin alms bowl, behind the seedling age 20d, spray with containing different disposal liquid respectively, each is processed and repeats 3 times.Treatment fluid sprays once every 5d, samples behind the 60d, measures and respectively processes green vegetables relevant physiological biochemical indicator.(annotate: contrast CK is clear water)
Assay method:
Fresh weight is measured: after the off-test, then 3 more consistent seedling of upgrowth situation of the random picking of every basin are cleaned with deionized water and blot its fresh weight of rear measurement.
Protein content determination: the method with reference to Bradford is measured.(Bradford?M?M.A?rapid?and?sensitive?method?for?the?quantitation?of?microgram?quantities?of?protein?using?the?principle?of?protein-dye?binding.Ann.Biochem,1976,72:248-254.)
Content of nitrite is measured: with reference to standard GB/T/T 15401-1994 " mensuration of fruit and vegetable and goods thereof---nitrite and nitrate content ", get green vegetables sample 2~3g, be ground to the homogenate shape, under weak basic condition, from sample, extract nitrite ion (NO with hot water 2 -), with potassium ferrocyanide and zinc acetate protein precipitation, filter to get transparent colourless solution, add again sulfanilamide (SN) and naphthodiamide hydrochloride, measure the absorbance of the red complex that generates at wavelength 538nm place, original nitrite ion (NO in the calculation sample 2 -) content.
Vc assay: get about testing sample 2g, add a small amount of 2% hydrochloric acid, in tissue mashing machine, make homogenate, move in the 100ml volumetric flask, to scale, absorbent cotton filters with 1% oxalic acid constant volume, get 10ml filtrate, with demarcated 2,6-dichloroindophenol solution titration determination Vc content carries out with reference to the method for Gao Peng.(high friend. the agrochemistry common analysis. Xi'an: Shaanxi science tech publishing house, 1980)
Soluble sugar content is measured: soluble sugar is measured and is adopted the anthrone colorimetric method, carries out with reference to the method for Zou Qi.(Zou Qi. plant physiology experiment instructs. Beijing: Chinese agriculture publishing house, 2000.)
. table 1 different disposal is on the impact of capsicum biomass and quality
Figure BDA0000116576970000051
As shown in Table 1, capsicum fresh weight, output, plant height, protein content, Vc content and soluble sugar content after formula I is processed have increased by 13.5%, 25.5%, 17.8%, 23.5%, 14.2% and 39.9% than contrast respectively, and content of nitrite has increased by 5.1% than contrast; Fresh weight, output, plant height, protein content, Vc content and soluble sugar content had increased by 25.6%, 40.3%, 24.4%, 44.2%, 40.8% and 54.1% than contrast respectively after formula I I processed, and content of nitrite has reduced by 8.3% than contrast.
On having as can be known, with formula I I process to capsicum biomass and quality to improve effect best.
Embodiment 7: based on the botanical sterilization preparation of thymol at the preventive and therapeutic effect to pepper anthracnose.
Formula I: 70% thiophanate methyl wetting powder.
The botanical sterilization preparation prescription of the thymol of formula I I: embodiment 1.
Choose three processing of morbidity dimension design of farmland: process 1: contrast (contrast CK is clear water); Process 2: formula I; Process 3: formula I I.Each process to repeat 3 times, and treatment fluid sprays once every 5d, carries out statistical analysis after spraying 3 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. Henan Agricultural Sciences, and 2010,4:77-79.)
As shown in Table 2, on capsicum, formula I is 54.76% to the preventive effect of pepper anthracnose; Formula I I is 85.05% to the preventive effect of pepper anthracnose.
As from the foregoing, process best to the control efficiency of pepper anthracnose with formula I I.
Table 2 different disposal is to the pepper anthracnose control efficiency
Figure BDA0000116576970000061

Claims (2)

1. the application of botanical sterilization preparation in promoting chili growth;
Wherein, described botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1 ~ 50%, emulsifier 5 ~ 30%, azone 0.1 ~ 20%, ethanol 10 ~ 60%, water 0.1 ~ 84.8%;
Described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
2. the application of botanical sterilization preparation in the control pepper anthracnose;
Wherein, described botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1 ~ 50%, emulsifier 5 ~ 30%, azone 0.1 ~ 20%, ethanol 10 ~ 60%, water 0.1 ~ 84.8%;
Described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
CN 201110400391 2011-12-06 2011-12-06 Botanical sterilization preparation and application thereof for controlling pepper anthracnose Expired - Fee Related CN102365940B (en)

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CN102365939B (en) * 2011-12-06 2013-04-17 江苏省农业科学院 Botanical sterilization preparation and application thereof for controlling anthracnose of grape
CN109258637A (en) * 2018-10-17 2019-01-25 四川省兰月科技有限公司 Sterilize the composition pesticide of control shoot

Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2001316214A (en) * 2000-05-11 2001-11-13 Sadayoshi Usuda Natural drug against plant disease
CN1357229A (en) * 2000-12-13 2002-07-10 刘岱 Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001316214A (en) * 2000-05-11 2001-11-13 Sadayoshi Usuda Natural drug against plant disease
CN1357229A (en) * 2000-12-13 2002-07-10 刘岱 Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent

Non-Patent Citations (2)

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Title
《百里香酚抑菌活性初探》;张静等;《中国农学通报》;20091231;第25卷(第21期);277-280 *
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